LOCUS       DASPKG010000001        11848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_271262, whole genome shotgun sequence.
ACCESSION   DASPKG010000001 DASPKG010000000
VERSION     DASPKG010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11848)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11848
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271262"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VEA36_00005"
     CDS             <1..994
                     /locus_tag="VEA36_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HYF28736.1"
                     /translation="GSIETGGIIMDPATGEIIALDTVPTFDANDFQNGDPAHFGNPLV
                     EHQYEFGSIVKALTMAAGLDAGVVAADSTYNDTGCMTLNNKKFCNYDLKARGTTPMQE
                     ILSQSLNMGATFIALRVGHEKFREYFKQLGMGEETGIDLPSEVPGNINNILRSPRDVE
                     YATASFGQGVAQTPVEMVKALGALANEGKVVTPHLVRAVKLESGIEKKLEWGGEERVF
                     SKEATLDTTRMLVKVVDVKLDGGKVKIPSMSVAAKTGTAQIAGPGGKYYENLYLHSFF
                     GYFPAYEPRFIILLYTRQPQGVQYASETLTHPFMELTHFLINYYEIPPDRAEVT"
     gene            998..2278
                     /locus_tag="VEA36_00010"
     CDS             998..2278
                     /locus_tag="VEA36_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HYF28737.1"
                     /translation="MKSLFRGIIVFLLNALTRGILHKYRPQIVMVTGSVGKSSTKDAI
                     ATALAPSIYLRASTKSYNSDFGLPLTVIGRETAWGNPIGWLSILVEGLLLLIFPAHYP
                     KLLVLEVGADHPKDLQKLLKIATPDAVVVTRLPEVPVHVEAYEGPEEVREEEFAPAGA
                     LAHGAPLILAAEDTHAKKLARGIHATITTYGYGEEADVRITAPVFVADGADAGMHATL
                     SIAGHPYEVSTRGVIGEQQLYAPAAAVATALALGVPVKQALLGLAEYEPPPGRARILA
                     GVSGSLLIDDTYNSSPAAVEEALKSLTLVKGAKRRVAILGDMLELGRYSAKEHERIGV
                     IAAEYVDVLITVGHRAESIATAFLNAREGGTVEDYENSVLAAAAIPESIREGDVVLIK
                     GSQSMRMERITEALLANPEDVAKLPRQDAAWKRR"
     gene            complement(2275..3294)
                     /locus_tag="VEA36_00015"
     CDS             complement(2275..3294)
                     /locus_tag="VEA36_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYF28738.1"
                     /translation="MKLKLDRATLRQAAIYAVLLAVGILVGWLLHVRSTLLEYRPYRE
                     QSSGYHFISPLLFVDVDEEYAFPKFRGLKQTVTAYADETLASPAVEDLSIYFRNVNTS
                     QWIAVNPDERYAPGSMLKVITLMSVLRVAEDQPDLLGRRVVPRGIEEQFPTGHGLYEP
                     ENPVRSGRVYTVEELLRRLVVDSDNGANAALSQLIGEAKVLETYDDLQLPRPTRDTGY
                     TPREYSRVFRALYNGTYLSHDQSEAALKLLSTTSFTPGIVAGVPDGVVVSHKFGVRNE
                     AASSEAAAAGVFTKHELHDCGIVYHPRAPYFICVMTRGTDLAALEEAIKEASALVWKE
                     VERLN"
     gene            complement(3322..3396)
                     /locus_tag="VEA36_00020"
     tRNA            complement(3322..3396)
                     /locus_tag="VEA36_00020"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3361..3363),aa:Glu,seq:ctc)
     gene            complement(3463..4239)
                     /locus_tag="VEA36_00025"
     CDS             complement(3463..4239)
                     /locus_tag="VEA36_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28739.1"
                     /translation="MKRWVVIEKRIGETPLRALEAWRAASPAYAGIPASYAGRLDPMA
                     SGKLLILLGDECKKQERYTGLDKEYEIEVLLDIGTDTGDVLGVPTYVGTKTSVVPDAA
                     LRAVTGAHVVPYPAFSSRTVNGKPLFLHTLEGNPIKIPTHEETVYRIRRLSESRVSKE
                     DLKARIEDLLTYAPRSDEPSKALGADFRQDEIREAWGAVFAQMPDRSFTILRLRVTCA
                     SGTYMRTLASRFGAQGLALSIHRTKIGQFLPFLGVWMRNF"
     gene            complement(4319..4687)
                     /locus_tag="VEA36_00030"
     CDS             complement(4319..4687)
                     /locus_tag="VEA36_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28740.1"
                     /translation="MSHHEPNPYLIHLKLLVLPPLMQGQPYYFPCVFTQAGIAQALRC
                     RQRFELYMELLKGHYRTPIELVRGPTGWFLDGHECPACPMDVARRVADEYKDTIKKCL
                     GLEHVVVVVPEERNILLRAA"
     gene            complement(4773..5231)
                     /locus_tag="VEA36_00035"
     CDS             complement(4773..5231)
                     /locus_tag="VEA36_00035"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HYF28741.1"
                     /translation="MSIHESIKAGIPEALRAKDEVRLRTYRSLAAAMTNEVVAKKRKP
                     DEFLTDEEAMAVLKRAANQRKDSIQQFEAGGRSELAATEQEELAVIEALLPTQMGKEE
                     IEAIARAKMAEMGITDKKDMGRFMGALMQEFKGRADGGDVKAVVDSLFSS"
     gene            complement(5228..5521)
                     /locus_tag="VEA36_00040"
     CDS             complement(5228..5521)
                     /locus_tag="VEA36_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28742.1"
                     /translation="MYALRSRYWIIRDADEERLTRLTFHIEQGDYFPFLATVIGMLGE
                     TASRCGPAGMAQEQARFAEEMRKDLLHLHEHYTIAPRKVRLAYTKRKMVRFDA"
     gene            complement(5524..6261)
                     /gene="radC"
                     /locus_tag="VEA36_00045"
     CDS             complement(5524..6261)
                     /gene="radC"
                     /locus_tag="VEA36_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HYF28743.1"
                     /translation="MNTRYRIRDNVAVLDNPERRYVIALRDLPDDEKPRERLLAAGPR
                     SLTTAELLAVVFGNGTKKEGVLEMSARIMKDYGERSIMRQANAKKLAEDLDIPIVKAL
                     QVVAVAELGRRFHARNDLAVPTLRTAKEVYEYAIDMRALPKEHLRGLYLNNHYKVVRD
                     EVISIGTVDMNIVHPREVFRPALEYAAAAVILVHNHPSGNVEPSEADIAVTAQLIEAG
                     ALLGIDVIDHVIVTRGGFKSVAGPYRC"
     gene            6360..7409
                     /locus_tag="VEA36_00050"
     CDS             6360..7409
                     /locus_tag="VEA36_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HYF28744.1"
                     /translation="MDLLTIALILLGLFFVQGLGLYFILKRQAGNGAPDQTAPLLLQR
                     LEGLERSLDSRMAENSKQMHETVRAQLGESSRLIKEITQEITSVKEIGRETGKFAEQL
                     KNLQDILKNPKQRGILGEYYLETVLKNVLPPGMFQMQYGFTNGEIVDAVVFVNDKIVP
                     IDSKFSLDNYNRYVHAEGAERTLLEKTFVNDLKLRITETAKYIRPEENTMDFAFMFIP
                     SEGIYYDLLTNQVGAGEDENLIQRAASKYKVIIVSPTSFLAYLQTVLQGLRALQIEQK
                     AVEIQKRVGELGKHVGSYEEYYKKLGNALGTAVSHYNAGYKELGKIDKDVYRIAESKI
                     GVEVELLEKPGAAEV"
     gene            7490..7813
                     /locus_tag="VEA36_00055"
     CDS             7490..7813
                     /locus_tag="VEA36_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28745.1"
                     /translation="MRIPSTSLEIAPTFGALLIGFLASGIFWSVGFTIYAAATGLETR
                     NYSQFLMQEVGPELHYLTKPQASFTSFRGTLEDEEPKRAKPNMCLRARTPSGVATNRG
                     CRGQP"
     gene            7893..8210
                     /gene="rplU"
                     /locus_tag="VEA36_00060"
     CDS             7893..8210
                     /gene="rplU"
                     /locus_tag="VEA36_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HYF28746.1"
                     /translation="MELAVIKTGGKQYVVSPGDLVTIEKLPEEVKKGDAVTFDTVLMT
                     DDGSVTNIGTPVVKGASVTGTVHSVGRAKKIDVVKYKAKSRYLKRRGHRQPYVKVKIE
                     KIA"
     gene            8292..8528
                     /locus_tag="VEA36_00065"
     CDS             8292..8528
                     /locus_tag="VEA36_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28747.1"
                     /translation="MLIPFEARDEKGRSAYHEFDLPAEPKLNQPLGINGQHGVPPGAA
                     VRAIMAETPRRIIFEVVSGSLDRMVAEFGWELAR"
     gene            complement(8581..9186)
                     /locus_tag="VEA36_00070"
     CDS             complement(8581..9186)
                     /locus_tag="VEA36_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HYF28748.1"
                     /translation="MSDRIEELARAFERFPGIGPRQAKRFVYHLLSSAQADRARLADL
                     LLNLGSDVRQCAMCMRFSPANTEGLCSYCADTSRDDALLMLVEKDQDLAAVERANAYR
                     GRYFVLGGVLTLSGKGQIRERELVRVVEERAKHGLSEIVLALSATSEGEHTADRIREL
                     LSPLRGSIRLSKLGRGLATGSELEYSDAETLSGALQNRKET"
     gene            complement(9190..11832)
                     /locus_tag="VEA36_00075"
     CDS             complement(9190..11832)
                     /locus_tag="VEA36_00075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF005852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HYF28749.1"
                     /translation="MADKALRVPPQNLEAERALLGALLLKPDVIHDVADTVKAESFYA
                     EKHRLIFEAMRELTERSEPIDILTLSDRLQTNGNLERVGGRAYIAELAGEAPAPGNYA
                     HYAELVARKHVMRSLIDTAYEITERAFDDGTDTAEVLDLAEKSIYAIGNSSAAHKFTA
                     IADKLEDAWERIENMSKNKDAIRGVPTGFPELDNLLSGFHPSDLVILAARPSVGKTSL
                     ALDIARNAAVKHGVPVGIFSLEMSSEQIVDRMLSAESFIDSWKMRTGAIHAEEDFGKL
                     RDALESLSKAPIYIDDTPGSNILAMRAVARRLKRERGIGILIVDYLQLMAPTNTKQSD
                     SMVQQVTEISRSLKSLARELNVPVIALSQLSRAVEQRGGKPRLSDLRDSGCLTGDTLI
                     QRADTGARVPIRDLVGQANVPVVSMDASYQLRPMLASKIFSSGVKPVFGLTTRSGRTV
                     KASGNHQFLTMQGWQRLDALAEGDRLALPRLLTAEAPPSVMSADELMLLAHLLGDGCV
                     LPRQPIHYTSADEANLDAVEGAAARLFGIVPRRVKQKNWWHSYLPSPHRLTHGKPHPI
                     TAWYYSLGIEPVRSYEKRVPQAVFAADSMSIQLFLHHLWATDGNISWKRLPGRKPAAS
                     IYYSTTSRGLAEDVQHLLLRLGIWSTIRTVTQGAHRPSHQVHVQSTPVQLRFLELVGC
                     YGARGAIVPELTAALREIAPLPNTDTIPKEVWDLFVTPGKQEADMSWREIASAMETAY
                     NGSALMATGVSRARLAKVATILKSEKLVHLAESDVYWDEIASITPLGEEEVFDATVPG
                     THNFVAGDVIVHNSIEQDADVVMFIHREDKRNPESDRPNIAEILIEKHRNGPTGKVEL
                     YFDEKRTSFMSVDKSGFGELAGEF"
BASE COUNT         2166 a         3753 c         3808 g         2121 t
ORIGIN      
        1 cggctcgatc gaaaccgggg gtatcatcat ggatcccgcg accggggaga tcatcgccct
       61 ggataccgtc cccaccttcg atgcgaacga tttccagaac ggcgatccgg cgcacttcgg
      121 gaacccgctg gtcgagcacc agtacgagtt cggctcgatc gtgaaggccc tcaccatggc
      181 ggcagggctc gacgccggcg tcgtcgcggc cgattccacc tacaacgata cgggctgcat
      241 gaccttgaac aacaagaaat tctgcaacta cgatctcaag gcccgcggga ccacccctat
      301 gcaggagatt ctctcgcagt ccttgaacat gggcgcaacc ttcatcgcgc tccgggtcgg
      361 gcatgagaag ttccgggaat acttcaagca gctcgggatg ggggaggaga cgggcatcga
      421 cttgccaagc gaagtgccgg ggaacatcaa taacatcctg aggagtcccc gggacgtcga
      481 gtacgccacc gcctccttcg gccagggcgt cgcgcagacc ccggtcgaga tggtgaaggc
      541 gctcggcgcg ctcgccaacg aagggaaggt cgtgacgccg cacctcgtgc gcgccgtgaa
      601 gctcgagagc ggcatcgaga agaagctcga gtggggcggg gaggagcggg tgttctcaaa
      661 ggaggccacc cttgacacca cccgcatgct cgtcaaggtg gttgacgtaa agctcgatgg
      721 tggcaaggtg aagattccct ccatgtcggt cgcggccaag acgggcaccg cgcagatcgc
      781 ggggccgggc ggcaaatact atgagaacct ctacctccac tccttcttcg gctatttccc
      841 ggcctacgag ccccggttca tcatcctcct ctatacccgc cagccgcagg gggtccagta
      901 cgcctccgag accctcacgc atcccttcat ggagcttacc cacttcctca ttaactatta
      961 cgagatacct ccggaccggg ccgaggttac ctagggtatg aaaagcctct tccgcggcat
     1021 catcgtattc ctcttgaacg cgctcacgcg gggcatcctc cataagtacc gcccccagat
     1081 cgtcatggtg accgggagcg tggggaagag ctcgaccaag gacgcgatcg ccaccgcgct
     1141 tgcgccttcg atatacctgc gcgccagcac gaagagctac aacagcgatt tcggcctgcc
     1201 cttgaccgtg atcggccgag agaccgcctg gggcaacccc atcggctggc tctccatcct
     1261 ggtggagggt ctcctgctcc tcatcttccc cgcgcactat ccgaagctcc tggtccttga
     1321 ggtaggggcc gaccatccga aggacctcca gaagctcctg aagatcgcaa cgcccgatgc
     1381 cgtcgtggtg acgcggctcc ccgaggtgcc ggtgcatgtc gaagcgtacg agggcccgga
     1441 ggaggtgcgc gaggaggagt tcgcgcccgc aggggcgctc gcgcacggcg cgccgcttat
     1501 ccttgcggcc gaggacaccc atgcgaagaa gctcgcccgc ggcatccacg ccaccatcac
     1561 gacctacggg tacggggaag aggcggacgt gcgtatcacg gcgccggtct tcgtcgccga
     1621 cggcgcggac gctggcatgc acgcgaccct ttccatagcc gggcacccgt atgaggtttc
     1681 cacgcgaggg gtgatcggcg agcagcagct ctatgcgccc gccgctgcgg tcgcgaccgc
     1741 gcttgcgctt ggcgtgccgg tgaagcaggc gctccttggc ctcgccgaat acgagccgcc
     1801 cccgggccgc gcccgcatcc tcgcgggtgt ctcaggttcg ctcctcatcg atgacacgta
     1861 caactcatcc ccagctgcgg tcgaggaggc gctcaaatcg ctcacgctcg tgaagggagc
     1921 gaagcgccgc gtcgccatct tgggggacat gctcgagctc ggccgctact ccgccaagga
     1981 gcatgaacgc atcggggtga tcgccgctga atacgtcgat gtgctcatca ccgtcggcca
     2041 ccgggcggaa tccatcgcga ccgcctttct gaacgcgcgg gagggaggga ccgtcgagga
     2101 ttacgagaac tcggtccttg cggccgcggc catcccggag agcatccggg aaggggacgt
     2161 ggtcctcatc aagggctcgc agagcatgcg catggagcgg atcacagagg cgctgcttgc
     2221 gaatcccgaa gacgtagcga aattgccgcg gcaggacgcg gcgtggaagc ggcgctagtt
     2281 cagccgctcc acttccttcc ataccaaggc cgatgcctct ttgatcgctt cctcgagcgc
     2341 ggccaagtcc gtgccgcgcg tcatgacgca gatgaagtac ggggcgcggg gatgatagac
     2401 gatcccgcag tcatggagct catgcttcgt gaagacccct gcggcggcag cttcgctcga
     2461 ggcggcttcg ttgcgcaccc cgaacttgtg cgagaccacg acgccgtcag ggaccccggc
     2521 gacgatgcct ggggtgaagc tggtggtgga gaggagcttc agcgccgcct ccgactgatc
     2581 atgcgagaga tacgtaccgt tatatagggc gcggaagacc cgtgaatatt cccgcggggt
     2641 gtaccccgtg tcccgggtgg ggcggggaag ctggagatcg tcataagtct cgagcacctt
     2701 cgcctccccg atgagctgcg agagggcggc gttggcgccg ttgtcggaat cgaccacgag
     2761 gcgccgcagg agctcttcga cggtgtagac gcggccggag cgcaccgggt tctcgggctc
     2821 atagaggcca tgacccgtcg ggaattgctc ctcgatgccg cggggcacga cgcgccgtcc
     2881 gagcaggtcc ggctggtcct ccgccacccg gagtacggac atgagcgtga tgaccttcag
     2941 catgctgccc ggggcatagc gctcgtccgg gttcaccgcg atccactggc tcgtgttcac
     3001 attgcggaag tagatcgaga gatcctccac ggcgggggag gcgagcgtct cgtccgcata
     3061 cgcggttact gtctgcttca acccgcggaa tttcgggaag gcatactctt cgtccacgtc
     3121 cacgaacagg agcggggaga tgaaatggta gcccgaggat tgctcgcggt atggacggta
     3181 ctccaagagg gtggagcgca catgcagcag ccagccgacg aggatgccga cggcgagcag
     3241 caccgcatat atcgccgcct gccgcagggt ggcgcgatcg agcttgagtt tcatatccct
     3301 atcttatacc aggaaccgct ctggtgactc cacggggaat cgaaccccga tttgatccgt
     3361 gagagggatc tgtcctaacc gttagacgat ggagccttgc gacgttttat accatacccg
     3421 ccagcgcgta ccaagagacg gccgcggtgg acgcattttt tcctagaagt tccgcatcca
     3481 taccccgagg aagggaagaa actggccgat tttcgtccgg tggatcgata gcgcgaggcc
     3541 ctgcgccccg aatcgtgacg cgagcgtgcg catgtacgtg ccggatgcgc aggtgacccg
     3601 aaggcggagg atggtaaatg agcggtccgg catctgcgcg aacaccgcac cccatgcctc
     3661 ccgtatctcg tcctgccgga aatccgcccc gagcgccttc gagggctcat cgctgcgggg
     3721 cgcgtatgta aggagatcct cgattcgggc tttaaggtct tccttcgata cccgcgattc
     3781 actcaatctc ctgatacggt acaccgtctc ctcatgcgtc gggatcttga tcggattccc
     3841 ctcgagcgta tgaaggaaaa gcggctttcc attcacggtc ctcgaggaga aggcaggata
     3901 ggggacgaca tgcgcgccgg taacggcccg aagcgccgcg tcaggcacga cgctggtctt
     3961 ggtgcccaca tacgtcggca ccccgagcac atccccggta tccgttccga tatcaagcag
     4021 cacctcgatc tcatactcct tatcgagccc cgtataccgc tcctgcttct tgcattcatc
     4081 cccgaggagg atgaggagtt tgccggaggc catagggtcg agccggcccg cgtagcttgc
     4141 tggtatgccc gcatacgccg gactcgcagc gcgccacgct tcgagcgctc taagtggcgt
     4201 ctccccgatc cttttttcga tcaccaccca tcgcttcatc gcttgatact accctaaagc
     4261 aaaacgccgc ctcgtcgcgc acccgtgggt gcgaaacaag gcggcgtccg gcggaccttc
     4321 aggcagcccg cagcaggatg ttgcgttctt ctggtaccac caccaccacg tgttcgagtc
     4381 cgagacactt cttgatcgtg tccttgtatt catcggcgac cctgcgggcc acgtccatcg
     4441 gacaggccgg gcattcgtgc ccgtcgagga accagccggt gggccctcgg accagttcga
     4501 tcggcgtccg atagtgcccc ttgagcagtt ccatgtagag ctcgaagcgc tgccggcacc
     4561 gaagcgcctg ggcgattccc gcctgcgtga agacgcaggg gaagtagtac ggttgcccct
     4621 gcatgagcgg gggcagcacg aggagcttca gatggatcag gtacggattc ggttcgtggt
     4681 gtgacatgag taactccgat tgaacgtcga agaaacccac catttagtgt gaaaggtctg
     4741 cgcccttcct gggcgggact ataccacgag gactatgaag agaaaaggga gtccacgacc
     4801 gccttcacgt cgcccccatc cgccctgccc ttgaattcct gcatcagggc gcccatgaag
     4861 cggcccatgt cctttttatc ggtgataccc atctccgcca tcttggcgcg ggcgatcgct
     4921 tcgatctcct ccttgcccat ctgcgtcggc agcagggcct cgatgaccgc gagctcctcc
     4981 tgctcggtcg cggcgagctc gcttcgcccg cccgcctcga actgctggat cgagtccttg
     5041 cgctggttgg ccgcgcgctt gaggaccgcc atcgcctcct cgtccgtgag gaactcgtcg
     5101 ggcttgcgct tcttcgctac gacctcgttg gtcatcgcgg ccgcaaggga ccggtaggtg
     5161 cgcagccgaa cctcgtcctt ggccctcagg gcctccggga tgcccgcctt gatggattca
     5221 tggatgctca tgcatcgaat cgtaccatct tccgcttcgt gtaggccagg cgcaccttcc
     5281 gaggcgcgat cgtatagtgc tcatgcagat ggaggaggtc cttgcgcatc tcctcggcga
     5341 accgggcctg ctcctgagcc atcccggcgg ggccacagcg cgacgccgtc tccccgagca
     5401 tgccgatcac ggtcgcgagg aaggggaagt aatcgccctg ctcgatatgg aacgtaagcc
     5461 gggtgagccg ctcctcgtcg gcatcccgga tgatccagta acgggagcgg agcgcataca
     5521 tggctagcag cgatagggac cggcgacgct cttgaagccg ccgcgcgtca cgatgacatg
     5581 atcgatcaca tcgatgccga gcagggcgcc ggcctcaatg agctgcgccg tcaccgcgat
     5641 atccgcctcc gacggctcca cgttcccgga cggatggttg tgcaccagga tgacggccgc
     5701 agcggcatac tcaagcgcgg gccggaacac ctcccgcggg tgcacgatat tcatatccac
     5761 cgtcccgatg gagatgacct catcgcgcac caccttgtag tggttgttga gatagaggcc
     5821 gcgcagatgc tccttcggga gcgcacgcat gtcgatggcg tactcgtaga cctccttcgc
     5881 ggtccgcagc gtcgggacgg cgaggtcgtt ccgggcatgg aagcgcctgc cgagctcggc
     5941 gaccgcgacc acctggagcg ccttgacgat cgggatgtcg agatcctccg cgagcttctt
     6001 cgcgttggct tgccgcatga tgctgcgctc cccgtagtcc ttcatgatgc gggccgacat
     6061 ctcgagcacg ccctccttct tcgtgccgtt gccgaatacg acggcaagca gctccgcggt
     6121 cgtgaggctg cggggccccg cggccaggag gcgttcgcgc ggcttctcat cgtccgggag
     6181 gtcccggagc gcgatgacgt accggcgctc ggggttatcg agtacggcga cattgtcgcg
     6241 gatgcggtag cgggtattca tgtttccagc atacggagat atcccggaaa gtaatcaaca
     6301 ccgaaattca gcttttcgtt attttcctga accagcggcg ctcgcgatat actgggcgca
     6361 tggacctcct caccattgcg ctcatcctcc tcggcctctt cttcgtgcag gggctcggcc
     6421 tgtatttcat cctcaagcgg caggctggga acggggcccc ggaccagacc gcgcccctct
     6481 tgctccagcg tctcgagggc ctcgagcgct ccttggattc ccgcatggcg gagaacagca
     6541 agcagatgca cgagacagtc cgcgcccagc tcggggagtc ctcgcgcctc atcaaggaga
     6601 tcacccagga gatcacctcg gtgaaggaga tcggccggga gaccgggaag ttcgccgagc
     6661 aattgaagaa tctccaggac atcctgaaga acccgaagca gcggggcatc ctcggcgaat
     6721 attacctcga gacggtcttg aagaacgtgc tcccgcccgg catgttccag atgcagtacg
     6781 ggttcacgaa cggggagatc gtggacgccg tcgtgttcgt caacgacaag atcgtcccga
     6841 tcgactccaa gttctcgctc gacaactaca accggtacgt gcatgccgaa ggggccgagc
     6901 gcaccctcct cgagaagacg ttcgtgaacg acctgaagct ccgcatcact gagaccgcga
     6961 aatacatccg tcccgaggag aacaccatgg acttcgcctt catgttcatc ccttccgaag
     7021 gcatctacta cgacctcctc accaaccagg taggcgccgg ggaggacgag aacctcatcc
     7081 agcgggcagc ctccaagtac aaggtcatca tcgtgtcgcc gacctccttc ctcgcctacc
     7141 tccagaccgt gctgcagggc ctccgggcgc tgcagatcga gcagaaggcg gtggaaattc
     7201 agaagcgcgt gggagagctc ggcaagcacg tcggcagcta cgaggagtac tacaaaaagc
     7261 tcggcaacgc gctcgggacc gcggtctcac actacaacgc gggatacaag gagctcggga
     7321 agatagacaa ggacgtgtat cgcatcgcgg agtcgaagat cggggtggag gtggagctcc
     7381 tcgagaagcc gggagcggcg gaggtgtagc ccacttcctt catcgagggg gcgggtgtac
     7441 agtcggggta gggggaacag ccttccagta caagtcggag gggaattcca tgcgtattcc
     7501 gtccacctcg ctcgagatcg caccgacgtt cggtgctctg ctgattggct tcctcgcaag
     7561 cggaatcttc tggtcggtcg gcttcaccat ttatgccgcg gccacggggc tcgaaacgcg
     7621 caactactca cagttcctga tgcaggaggt cgggcccgaa ctgcactatc tgaccaagcc
     7681 ccaggcctcg ttcaccagct tccgcggcac tcttgaggac gaggagccga agcgagcgaa
     7741 gccgaacatg tgcctgcggg cacgcacgcc atcgggggtt gcgacaaatc gaggctgccg
     7801 aggacaacct tgagcgacaa gcagaccggc cgcacgggag aaccccgcgc ggccttgctt
     7861 tttggcccct ccacggctag gatgggaaaa ctatggaatt ggcggtcatc aagacgggcg
     7921 gcaagcagta cgtggtctcc ccaggggacc tcgtcaccat cgagaagctt ccggaggagg
     7981 taaagaaggg cgatgccgtc accttcgata ccgtcctcat gacggacgac ggctcggtca
     8041 cgaacatcgg caccccggtc gtgaaggggg cttccgtcac cggcaccgtc cactcggtgg
     8101 gccgcgcgaa gaagatcgac gtggtcaagt acaaggccaa gagccggtac ttgaagcgtc
     8161 gcgggcaccg ccagccctac gtgaaggtga agatcgagaa gatcgcgtaa ccccacagcc
     8221 ccgcccttgg cggggcttct ggtttccggt agggtagggg aaggtcaaac gagaacaggg
     8281 gcacgaagcg catgctgata ccgttcgagg cacgggacga gaaggggcgg agcgcctacc
     8341 acgagttcga tcttcctgcg gagccgaagc tcaaccagcc gctcggcatc aacgggcagc
     8401 acggggtccc gccaggcgcc gccgtccggg ccatcatggc cgagacgccg cgccggatca
     8461 tcttcgaggt cgtgagcggc agcctcgacc ggatggtcgc ggagttcggt tgggagctcg
     8521 cccggtaggt cccggggcgc actaccaggg ggtcgcgcat ggaaacgtgc gcgaccccgg
     8581 ttacgtttcc ttcctgttct gcagcgctcc ggacagggtc tctgcgtccg aatactcgag
     8641 ctcggagccg gtcgcaaggc cgcggcccaa tttggatagg cggatcgatc cccgaagcgg
     8701 ggagaggagc tcccggatgc gatctgcggt atgttcgccc tcgctcgttg ccgagagcgc
     8761 gagcacgatc tccgaaagcc cgtgcttcgc acgctcctcc actacccgca cgagctcccg
     8821 ctcccgtatc tgccccttcc cggacagggt aaggacgccg ccgagtacga aatatcgccc
     8881 gcggtatgcg ttggcgcgct ccacggccgc aaggtcctgg tccttctcga cgagcatgag
     8941 gagcgcatcg tcgcgggacg tatcggcgca gtaactgcag agcccttcgg tattggcggg
     9001 agagaagcgc atgcacatag cgcactggcg gacatcgctg ccgaggttca gtaaaaggtc
     9061 cgcgagccgt gcgcgatccg cctgggccga ggacaggagg tgatacacga agcgcttggc
     9121 ctgccggggc ccgatccccg ggaagcgctc gaaggcgcgc gcgagctcct cgatgcgatc
     9181 cgacatatcc tagaactcgc ccgcgagctc cccgaagccg gacttatcca ccgacatgaa
     9241 cgaggtgcgc ttctcgtcga agtagagctc gaccttgccg gtgggcccat tgcggtgctt
     9301 ctcgatgagg atctccgcga tgttggggcg atcgctctcg ggattgcgct tgtcctcgcg
     9361 atgaatgaac atcacgacgt ccgcatcctg ctcgatacta ttgtggacga tgacgtcgcc
     9421 ggcgacgaag ttgtgcgtgc cgggcacggt ggcgtcgaag acctcctcct cgccaagcgg
     9481 ggtgatggag gcgatctcgt cccagtacac gtcggattcc gcgaggtgca cgagcttctc
     9541 gctcttgagg atggtcgcga ccttcgcgag ccgcgcgcgc gatacccctg tggccattaa
     9601 ggcgcttccg ttataggcgg tctccatggc gctggcgatc tctcgccagc tcatgtccgc
     9661 ctcctgcttc ccaggcgtca cgaagagatc ccacacctcc ttggggatcg tatcggtgtt
     9721 cggcagcggg gcgatctctc ggagtgccgc ggtgagttca gggacgatgg cgccgcgcgc
     9781 gccgtagcag ccgacgagct cgaggaaccg cagctgtacc ggtgtgctct ggacgtgcac
     9841 ctgatggctc ggacggtgcg cgccctgcgt caccgttcgg atggtgctcc agatgccgag
     9901 ccgcaacagg aggtgctgca cgtcctcggc aagcccgcgg ctggtcgtcg aataatagat
     9961 agaggctgcc ggctttcgcc ccgggagccg cttccaggag atgttcccat ccgtcgccca
    10021 gaggtggtgc aggaagagct ggatggacat gctatcggca gcgaacaccg cctgcggcac
    10081 ccgcttctcg taggaccgca ccggctcaat gccgaggctg tagtaccagg cggtaatagg
    10141 gtgcggcttc ccgtgggtga ggcgatgcgg cgacggaagg tagctgtgcc accagttctt
    10201 ctgcttcacc cgccgcggga caatcccgaa gagccgtgca gccgcgcctt cgaccgcatc
    10261 gagattcgcc tcgtcggcgc tcgtgtaatg gatgggctgg cgcggcagca cgcagccgtc
    10321 gccgagcagg tgcgcgagca gcatgagctc gtcagcggac atgaccgacg gcggtgcctc
    10381 ggcggtcagc aggcggggaa gggcgagccg atcgccttcc gcaagggcgt cgagccgctg
    10441 ccacccctgc atggtgagga attggtggtt ccccgacgcc ttcaccgtgc ggccgcttcg
    10501 cgtggtcaat ccgaacaccg gcttcacgcc gctcgagaat atcttcgagg cgagcatggg
    10561 ccgcagctgg tacgacgcgt ccatagagac gacgggcacg ttcgcctggc cgaccagatc
    10621 gcgaatgggc acgcgcgcgc cggtgtcggc ccgctggatg agggtgtcgc cggtcaaaca
    10681 ccccgaatcg cgaaggtccg agagccgggg cttgccgccg cgctgctcga ccgcgcggga
    10741 gagctgggag agcgcgatga ccggcacgtt gagttcccgg gcaagggact tcagggaacg
    10801 cgagatctcg gtgacctgct ggaccatgga gtccgattgc ttcgtattgg tcggcgccat
    10861 gagctgaagg tagtcgacga tgaggatgcc gatgccgcgc tcgcgcttca accttcgtgc
    10921 caccgcgcgc atggcgagga tgttggagcc cggggtgtcg tcgatgtaga tgggcgcctt
    10981 cgagaggctc tcgagcgcat cgcgcagctt gccgaagtcc tcctcggcat ggatggcgcc
    11041 cgtgcgcatc ttccaggagt cgatgaagga ctccgccgag agcatgcggt ccacgatctg
    11101 ctccgagctc atctcgagcg agaagatgcc gaccggcacc ccatgcttga ccgccgcgtt
    11161 gcgtgcgatg tcgagcgcga gcgaggtctt accgaccgag ggtcgtgcgg cgaggatgac
    11221 gaggtccgag gggtggaagc ccgagaggag attgtcgagc tcggggaagc ccgtcgggac
    11281 gccgcgaatg gcgtccttgt tcttgctcat gttctcgatg cgctcccagg cgtcttcgag
    11341 cttgtcggcg atcgcggtga acttatgggc agcggacgag ttgccgatgg cgtagatgga
    11401 cttctcggcg aggtcgagca cctcggcggt gtccgtgccg tcatcgaagg cgcgctccgt
    11461 gatctcgtag gccgtatcga tgagggagcg catcacatgc tttctcgcca cgagctccgc
    11521 gtagtgcgcg tagttgcccg gtgccggcgc ctcgcccgcg agctccgcga tgtaggcgcg
    11581 gcccccgacg cgctccaggt tcccgttggt ctggaggcgg tccgagagcg tgaggatgtc
    11641 gatgggctcg ctccgctccg tgagttcccg catcgcctca aagatgaggc ggtgcttctc
    11701 cgcgtagaag gattcggcct tcaccgtgtc cgcgacgtcg tggatgacgt cgggcttcag
    11761 caacagagcg ccaagcagcg cgcgctcggc ttcaagattc tggggaggga cgcgcagcgc
    11821 tttgtcggcc attatgaggc cattctat
//
LOCUS       DASPKG010000002         4846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_677580, whole genome shotgun sequence.
ACCESSION   DASPKG010000002 DASPKG010000000
VERSION     DASPKG010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677580"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VEA36_00080"
     CDS             <1..490
                     /locus_tag="VEA36_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28750.1"
                     /translation="NPTMNGPIAAARQEILNYDNALAAAERFRAKEAELDAERRAIPP
                     ESLVRLESFLPDGVDNVQLILDLDALASRTGLTLAGFDVTEMQTTEEVDPNGIIISDD
                     LPTESIELTTTGTGSYESFRRFLTGAERSLRPLDIASLRITTSEAGVYTYNITFRLYW
                     LK"
     gene            493..798
                     /locus_tag="VEA36_00085"
     CDS             493..798
                     /locus_tag="VEA36_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28751.1"
                     /translation="MNLNALHISKTVAVLIILGIIVAAAIAFTLMKGDEPEETVTVSG
                     DSTSTAEANFVSLATELDSIVFDTAILSDPRFLALENIHTAITPEPQGRTDPFSSAD"
     gene            838..2643
                     /locus_tag="VEA36_00090"
     CDS             838..2643
                     /locus_tag="VEA36_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HYF28752.1"
                     /translation="MRYHERVAHSAAASMTTLSLLVDAGLLKQEDASSLTAEAKGSAT
                     SIETLLEKRGITIEQILEVKAKHYDLPYRILPAQEIAHEVLEFIPEESARHYGFVPLG
                     LKDAALEVGVVNPDNLEALDALQFISTRVGMPYKLYLISETDFARVIKEYEDLTGEVD
                     EALTEYEGALEKDKAEVIAGDAAVEQEAIKADAPVTKIVATILRYAVEGGASDIHIEP
                     TDEKTRVRFRMDGELHTSLELPAKVHPAVIARIKILSQMRLDEKRKPQDGRFSATVEG
                     RRIDFRVSTFPTADGEKVVMRILDRAKSIETLADLGLSPADLEKVQTILRQPYGIILV
                     AGPTGSGKSTTLFAMLSALDRETVNVVSIEDPVEYEIQGVAQSQVRPEIGYTFASGLR
                     SILRQDPDVIMVGEIRDKETAQLAVQAALTGHLVFSTIHTNTAAGVIPRLIDMGVDPY
                     LIAPTLIAAVGQRLVRKLCPGAGEAVPVSDSIRKLLEEEFADLPESAKKDIPEFKHIY
                     HAKPTPDCPNGTRGRLAVFEFLEMDADLEQVVLKNPVEESVYAAARAKGMYTMREDAI
                     MKALAGEIPFEEVNTLGGTYFAVEESEHLPPEAVL"
     gene            2640..3026
                     /locus_tag="VEA36_00095"
     CDS             2640..3026
                     /locus_tag="VEA36_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYF28753.1"
                     /translation="MNPYRLYLVDDDRFLLDMYAVKFRAAGHEVTAFSKGEELLAALR
                     EKPAPNALLLDVVMPGMSGFDILDTIRKEELAPEMKVIILSNQGQDADLNRAKELKAD
                     GYIIKASAIPSEVFSETIKIIETKAA"
     gene            3023..4081
                     /locus_tag="VEA36_00100"
     CDS             3023..4081
                     /locus_tag="VEA36_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HYF28754.1"
                     /translation="MSDTRIHEFLDTVVKEGGSDLHLSPGSPPIIRVSGALVPLVQES
                     VLTPADTAGMLKAVVPESMWEKFEADQAVDFSLAHTDGNRFRVNGYVVQGAVVIAMRL
                     IPRDIRTFETLNLPPVLEVFSQRQQGFFLVVGPVGQGKSTTLAAIIDRINETRAEHIV
                     TIEDPVEYIFEQKKSLIHQREVHIDTPDFAAALTSAFREDVNVIMVGEMRNYETISAA
                     VTAAETGHLVLSTLHTNNAAQTIDRIIDMFPPNQQGQVRVQLAGSLAGIFSQRLVPKI
                     SGGLVPAYELLINNSAIANLIREGRTHEISTVIQTSSQEGMIDMDRSLAELVRRGEVT
                     IEHAYERAMDPKTFERYL"
     gene            4085..>4846
                     /locus_tag="VEA36_00105"
     CDS             4085..>4846
                     /locus_tag="VEA36_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYF28755.1"
                     /translation="MLFSYRALDTDGHERQGTIEALSMDVAVGTLQRRNLIVSSIEPM
                     TKKGNLPISFNFFKPVSNKDVVILSRQIATLFEAQVSALRVFRLLAAEVDNRHLADIL
                     TQVADDLQGGSPISKALARHPMVFTSFYVNMVRSGEESGKLSETFGYLADYLDRTYEV
                     ASKAQNALIYPAFVITVFFVVMGLMLTMVIPKVSQILIDSGQEVPIYTKIVIGLSMFL
                     VNYGAFFLVALAVAGFFAFRFSQTPPGRLFFDDLKL"
BASE COUNT          907 a         1696 c         1429 g          814 t
ORIGIN      
        1 caacccgacg atgaacgggc cgatcgcggc tgcgcgccag gagatcctca actacgacaa
       61 cgcgcttgcc gccgccgagc gcttcagggc caaggaggcc gagctcgacg ccgagcgccg
      121 tgcgatcccg cccgaatccc tggtgcgtct cgaatccttc cttcctgacg gcgtcgataa
      181 cgtgcagctc atcctcgatc tcgacgcgct cgcgtcgcgg accgggctca cgctcgcggg
      241 tttcgacgtg acggagatgc agacgacgga ggaagtcgac ccgaacggga tcatcatctc
      301 ggatgacctg ccgaccgagt cgatcgaact cacgacaacc ggcacgggtt cctacgaatc
      361 gttccggagg ttcctcaccg gcgcggagcg gagcctgcgc ccgctcgaca tcgcgagcct
      421 gcgcatcacg acgagcgagg ccggcgtcta tacctacaac atcaccttcc gcctgtactg
      481 gctcaaatag ctatgaacct caacgcgctc catatctcga agaccgtcgc cgtcctcatc
      541 atcctcggca tcatcgtcgc ggcagccatc gccttcaccc tcatgaaggg cgacgagccc
      601 gaggagacgg tcacggtctc gggcgatagc acctcgaccg ccgaagcgaa cttcgtctcg
      661 ctcgcgaccg agctcgactc catcgtcttc gataccgcca tcctctccga tccgcgcttc
      721 ctcgcgctcg agaacatcca taccgcgatc acgcccgagc cccagggccg caccgatccg
      781 ttctcctccg cggactaacc acagctcgcc cgacggccgc gcgaaacacg cgcggccgtg
      841 cgttatcatg agcgtgtagc gcacagcgcc gcggcttcca tgaccactct ttcgctcctc
      901 gtcgatgcgg gactcctcaa gcaggaggat gcttcctcgc tcaccgcgga ggcgaagggc
      961 tccgcgacct ccatcgagac cctcctcgag aagcggggca tcaccatcga gcaaatactc
     1021 gaggtgaagg cgaagcacta cgacctcccg taccgcatcc tgcccgcgca ggagatcgcg
     1081 cacgaggtcc tcgaattcat cccggaggag tccgcccgcc actacggctt cgtgccgctc
     1141 ggcttgaagg acgcggcgct cgaggtcggc gtcgtgaacc cggacaacct cgaagcgctc
     1201 gacgcgctcc agttcatctc gactcgggtc gggatgccgt acaagctcta cctcatcagc
     1261 gagacggact tcgcgcgcgt catcaaggag tacgaggacc tcaccgggga ggtggacgag
     1321 gcgctgaccg aatacgaagg cgcgctcgag aaggacaagg cggaagtcat cgcgggcgac
     1381 gcggccgtcg agcaggaggc catcaaggcg gacgcgccgg tgacgaagat cgtcgctacc
     1441 atcctccggt atgcggtcga gggcggcgcc tccgacattc acatcgagcc caccgacgag
     1501 aagacccggg tgcgcttccg catggacggc gagctccaca cctcgctcga gcttccggcg
     1561 aaggtgcatc ccgcggtcat cgcgcgcatc aagatcctct cgcagatgcg cctcgacgag
     1621 aagcgcaagc cgcaggacgg ccgcttctcg gcgacggtgg agggccggcg catcgacttc
     1681 cgcgtctcga ccttcccgac ggccgatggc gagaaggtcg tgatgcgtat cctcgaccgc
     1741 gcgaaatcca tcgagacgct tgcggacctc gggctctcgc cggccgacct cgaaaaggta
     1801 cagacgatcc tccgccagcc ctacggcatc atcctcgtcg cggggcccac cggctccggt
     1861 aagtcgacga ccctgttcgc catgctctcg gcgctcgacc gcgagaccgt gaacgtcgtc
     1921 tcgatcgagg acccggtcga gtacgagatc cagggcgtcg cgcagtcgca ggtgcggcct
     1981 gagatcgggt acaccttcgc ctcggggctc cgctccatcc tgcgccagga cccggacgtg
     2041 atcatggtcg gcgagatccg cgacaaggaa accgcacagc tcgcggtcca ggccgcctta
     2101 accggccatc tcgtcttctc gaccatccat acgaacaccg cggccggcgt catcccgcgc
     2161 ttgatcgaca tgggcgttga cccctacctc atcgcgccga ccctcattgc cgcggtgggg
     2221 cagcgcctcg tccggaagct ctgcccgggc gcgggcgaag cggtgccggt ctccgactcc
     2281 atccggaagc tactcgagga ggagttcgcg gaccttcctg aatcggcgaa gaaagacatc
     2341 ccggagttca agcacattta tcatgcaaaa ccgacgcccg actgcccgaa cggcacccgc
     2401 ggtcgcctcg ccgtcttcga attcctcgag atggacgccg atctcgagca ggtggtgctc
     2461 aagaacccgg tcgaggagag cgtctacgcc gccgcgcgcg ccaagggcat gtatacgatg
     2521 cgcgaggatg ctatcatgaa ggcactcgca ggcgagattc cgttcgaaga ggtgaacacc
     2581 ctcggcggga cctacttcgc cgtcgaagaa tcagagcatt taccccctga agccgtccta
     2641 tgaatcccta ccgcctctat ttagtcgatg acgaccgctt cctcctcgac atgtacgccg
     2701 tgaaattcag ggcggcaggc cacgaagtca cggccttctc aaagggagag gagctcctcg
     2761 cggcgctccg ggagaagccg gcgccgaacg cgctcctgct cgacgtcgtc atgccgggca
     2821 tgagcgggtt cgacatcctc gataccatcc gcaaagagga actcgccccc gagatgaagg
     2881 tcatcatcct ctcgaaccag gggcaggacg cggacttgaa ccgggcgaag gagctgaagg
     2941 ccgacggcta catcatcaag gcatccgcga tcccgtcgga ggtcttctcg gagacgatta
     3001 agatcatcga aaccaaagct gcatgagcga tacccgcatc cacgaattcc tcgatacggt
     3061 cgtgaaggaa ggcggctccg acctccacct ctcgcccggg tccccgccca tcatccgcgt
     3121 ctcgggcgcg ctcgttccct tggtccagga atccgtcctg actcccgccg acaccgcggg
     3181 gatgctgaag gcggtcgtgc cggagtcgat gtgggagaag tttgaggccg accaggcggt
     3241 cgacttctcg ctcgcgcata ccgacggcaa ccgcttccgc gtcaacgggt acgtcgtgca
     3301 gggcgcggtc gtcatcgcca tgcgcctcat cccgcgcgat atccgcacct tcgagacgct
     3361 gaacctcccg ccggtcctcg aggtcttcag ccagaggcag cagggcttct tcctcgtcgt
     3421 ggggccggtg ggacagggca agtccacgac tttggcggcc atcatcgacc gcatcaacga
     3481 gacgcgcgcc gagcatatcg tcacgatcga ggacccggtc gagtacatct tcgagcagaa
     3541 gaagtccctc atccaccagc gcgaggtgca catcgatacg ccggacttcg ccgcggcgct
     3601 cacctccgcc ttccgcgagg acgtgaacgt gatcatggtc ggcgagatgc gcaactacga
     3661 gaccatctcg gccgcggtga ccgccgccga gaccgggcac ctcgtcctgt ccacgctcca
     3721 taccaacaat gcggcccaga cgatcgaccg catcatcgac atgttcccgc cgaaccagca
     3781 ggggcaggtg cgcgtgcagc tcgcagggtc cttggccggc atcttctcgc agcggctcgt
     3841 cccgaagatc tcgggcggcc tcgtcccggc ctacgagctt ttgataaaca acagcgcgat
     3901 cgcgaacctg atccgcgaag ggcgcacgca cgagatctcg acggtcatcc agacctcgag
     3961 ccaggagggc atgatcgaca tggaccgctc gctcgcggaa ctggtgcgcc gcggggaggt
     4021 caccatcgag cacgcctatg agcgcgcgat ggacccgaag accttcgaac gctatctcta
     4081 gcctatgctc ttctcctacc gcgcgctcga caccgacggc cacgaacggc aggggaccat
     4141 cgaggccctc tcgatggacg tcgcggtggg gaccctgcag cgccgcaacc tcatcgtctc
     4201 gtctatcgag ccgatgacga agaaggggaa cctcccgatc tccttcaact tcttcaagcc
     4261 cgtctcgaac aaggacgtgg tgatcctgtc gcgccagatc gcgaccctgt tcgaggcgca
     4321 ggtctcggca ctgcgcgtct tccgcctcct tgcggccgag gtcgataacc ggcacctcgc
     4381 ggacatcctc acgcaggtcg ccgacgacct ccagggcggc agccccatct ccaaggcgct
     4441 cgcgcgccat cccatggtct tcacctcctt ctacgtgaac atggtccgct cgggcgagga
     4501 gtcgggcaag ctctcggaaa ccttcgggta tctcgccgac tacctcgacc gcacctacga
     4561 ggtggcatcg aaggcgcaga acgcgctcat ttacccggcc ttcgtgatca cggtgttctt
     4621 cgtggtgatg ggcctcatgc tcaccatggt gatcccgaag gtgagccaga tcctcatcga
     4681 ttcggggcag gaggtcccca tctacacgaa gatcgtcatc ggcctctcga tgttcctcgt
     4741 caattacggc gccttcttcc tcgtggcgct cgcggtcgcc gggttcttcg cgttccgctt
     4801 ctcgcagacg cctcctggcc gcctcttctt cgacgatctg aagctc
//
LOCUS       DASPKG010000003         7012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1222826, whole genome shotgun sequence.
ACCESSION   DASPKG010000003 DASPKG010000000
VERSION     DASPKG010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222826"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VEA36_00110"
     CDS             <1..1293
                     /locus_tag="VEA36_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HYF28756.1"
                     /translation="GTFDVSVLEVGDDVIEVKSTDGDSHMGGRDIDQKIVRYLADEFK
                     KESGIDVTKDPLALQRLDEGAEKAKHELSSASETEVNIPFITVDGSGNPQHLLVKLTR
                     ATLESLAEEYVTRSIDITKRALEASGMQPSDINEVILVGGQTRMPKIQEEVEKLFGKK
                     PHRDVNPDEVVAIGAAIQAGILQGDVKDILLVDVIPLSLSIETMGGVATKLIERNTAI
                     PTSRSQVFSTASDNQTNVEIHITQGERAMSADNKSLGRFMLEGIPPAPRGMPQVEVTF
                     DVDSNGILTVKAKEKTTGKEQSIRIEGSTGLSKEDIEKMQADAAAHAAEDQKRKDLVE
                     ARNVAEQLIYTAEKAVKENGEKAGPDVVAEVNEKIAALRTAREGEDSEAIKSASEALS
                     ASLSKIGEAMMKQDQKERPAEPGDQKPDENPKDADFTE"
     gene            1346..1759
                     /locus_tag="VEA36_00115"
     CDS             1346..1759
                     /locus_tag="VEA36_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28757.1"
                     /translation="MKLHIIGCVGALALAAAACGKPVPAHAPYDSEQERQAVLRRFDP
                     FCMARVGYDYRPCAAVIRFQRLGGHELYGLVLGVDRTRVHVRWGHALPDQFDHVALET
                     TEMPGRIVQIERLASPAGQRLLAEYERGRVPPPTK"
     gene            1824..2297
                     /locus_tag="VEA36_00120"
     CDS             1824..2297
                     /locus_tag="VEA36_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28758.1"
                     /translation="MAYMTRVAIPAREEKRMELLALNPHGDLEIARRQAQDLCAALQE
                     VFDEFPIGVAMHLVCDELRNAGESEVSLILIDVTGPGMEDYGLLTIDMPCGSRLEEFR
                     SWGIRVQYQRPPVRLEAHFVGHDGGPTIREFIRCKIAIQDILREDLERQRVKRSS"
     gene            2400..3452
                     /locus_tag="VEA36_00125"
     CDS             2400..3452
                     /locus_tag="VEA36_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HYF28759.1"
                     /translation="MSKNYYDILGVDKKASKEEIKKAFRKLAQKHHPDKGGSEEKFKE
                     ITEAYATLADEKRRREYDSYGQTFGAGGAGGFDFSGFQNGSVEFDLSDLFEGFGDIFG
                     TGTRGPRMRRGRDISIDIEVSFKDAVLGTKRQVLITKVGKCDICQGSGGKPGTQMESC
                     TTCNGAGRVHETRNSPFGTFASVRSCPTCEATGKVPKEKCGTCAGRGVLKKEEELKIA
                     IPAGIDGGEMIRMPGMGEAVKGGVSGDLYVKVHVKPHPVFKKHGLNLVMDLPVKLTDA
                     LLGTTASVETIEGKKLEVKIPPMAAPEETLRIKGKGVRVDDAQGDLLIKLTVVLPKKL
                     SARAKRAFEELKGEGL"
     gene            3473..4000
                     /locus_tag="VEA36_00130"
     CDS             3473..4000
                     /locus_tag="VEA36_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28760.1"
                     /translation="MDVTSVATQVSDLATSGAANAWLLAGNFVILLVLFIVMLLFSYK
                     AGRGGIISLIVAFYAGYAIYMVFPYTQMVVNAGGTPLVKAVISVAVFAIATFIPFHFV
                     QRLASGGFGVLSVVPRFVLSFLAAAFLLALAYHVFDISDIYTFPAPIDKLFAPEGYFF
                     WWFIAPMAGLLFLVH"
     gene            4048..5829
                     /locus_tag="VEA36_00135"
     CDS             4048..5829
                     /locus_tag="VEA36_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYF28761.1"
                     /translation="MSDDTKREIVPAGRILRAYAPELGKHPGLFVMVMVGMAGIVATD
                     LIAPLYLKQFFNAIAGTDPTTNLVAELSMIITIVIALYVANWVAFRAYSIGLINLELK
                     AMSRLYNHAFDYLIRHSQHFFTSQFTGTLTRRVAKFSDAFETLVDTVFMQFVPTAIFV
                     VGAVIVLATQHPWLGGILAVWSVIFIIVQIWLANLRQPLREAQSRAESERTGATADAL
                     SNQSAITQFAAEEAESRKLADVVARWRQAAMRSWGADEFIWAVLGLLMIGIQAALLYA
                     AVELWSRGLLTVGDFVLIQAYLITTFERLIGINRDLRRFYAGLANASEMITILDTDYD
                     IKDAAGARELVVDEGSITFEDVSFGYQDRPTLAGFSLSIPSHQKVALVGPSGAGKSTI
                     TKLLLRLYDVTGGTIRIDGQDIREVTQESLRKAISFVPQEPALFHRSLRENISYGRPD
                     ASEAEIIEAAKKAHCHEFIAGLPLGYETYVGERGVKLSGGERQRVAIARAILKDAPIL
                     VLDEATSSLDSESESLIQDALRTLMAGKTVVVIAHRLSTIMSMDRIVVLEGGRVVADG
                     THDELVADAGGLYHKLWSIQAGGFIAG"
     gene            5848..6771
                     /locus_tag="VEA36_00140"
     CDS             5848..6771
                     /locus_tag="VEA36_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYF28762.1"
                     /translation="MPPYLSGILVGFLEPVLHAWSNIIDNHFSNAVFPRLPVLLFFGV
                     ALNIVFLPITFLAGLPVMLPVTLLGIVFVIAIVNVVYAFPYYLALRDADTSVVASLFS
                     LGRIFVPVLAYFILGEVLTLPQYAGFFVIILSSALLTLDLKKLKVNRAFFLMLACSFV
                     LAFQTILYKFVFDAGASWPSVVAALTVFELVIAGAIMFATTSLRHIREDLGTVRRNWK
                     LFVAQQGLEWGGNVGASYAISILQVTVAQAIASTQPFFVLLYAVLFKKWFPNAFNETT
                     ARGDLLKKGILFACIILGTVLVVAHGPVIDV"
     gene            6798..>7012
                     /locus_tag="VEA36_00145"
     CDS             6798..>7012
                     /locus_tag="VEA36_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28763.1"
                     /translation="MLIGERLMYGAMIAMVVGAISWTAWLYLGEKEYPFIVEAACDPS
                     VETCFHRACSDTSNTGDCPPNALEDYK"
BASE COUNT         1290 a         2168 c         2274 g         1280 t
ORIGIN      
        1 ggcaccttcg acgtctctgt gcttgaggtg ggtgacgacg tgatcgaggt gaaaagcacc
       61 gacggcgaca gccacatggg cggccgcgac atcgaccaga aaatagttcg ataccttgcg
      121 gacgaattca agaaggagag cggcatcgac gttacgaagg acccgcttgc gctccagcgc
      181 ctcgatgagg gggcggagaa ggcaaagcac gagctctcga gtgcaagcga aaccgaggtg
      241 aacatcccgt tcattacggt ggacggcagc ggcaacccgc agcaccttct cgtgaagctc
      301 acgcgggcga cccttgagtc gctcgccgag gagtacgtca cccgctcgat tgatatcacg
      361 aagcgcgcgc ttgaggcttc aggcatgcag ccaagcgaca tcaatgaggt gattctcgtg
      421 ggcggccaga cccgcatgcc gaagatccag gaggaggtgg agaagctctt cgggaagaag
      481 ccgcaccggg acgtgaaccc ggatgaggtg gtcgccatcg gtgcggcgat ccaggccggc
      541 attctccagg gcgacgtgaa ggatattctc cttgtggacg tgattccgct ctccctttct
      601 atcgaaacca tgggaggagt cgcgaccaag ctcatcgagc gcaataccgc gatcccgacc
      661 tcgaggagcc aggtcttctc gaccgcatcc gacaaccaga cgaacgtcga gatccacatc
      721 acccagggcg agcgcgcgat gtccgcggac aacaagtcgc tcggccgctt catgctcgaa
      781 ggcattccgc cggctccgcg cggcatgccg caggtcgagg tgacctttga cgtggacagc
      841 aatggcatcc tcacggtgaa ggccaaggag aagacgaccg gcaaggagca gtcgatccgc
      901 atcgagggct cgactggact ctccaaggaa gacatcgaga aaatgcaggc tgatgccgcc
      961 gcgcatgccg ctgaggacca gaagcgcaag gacttggtcg aggcgaggaa cgtcgccgag
     1021 cagctcatct ataccgcaga gaaggcagtg aaggagaacg gggagaaggc agggcctgac
     1081 gtggtcgcgg aggtgaacga gaagatcgct gcgctccgga ccgcgcgcga aggcgaagat
     1141 agcgaagcca tcaaatcggc ttcagaggcg ctctctgctt cgctctcgaa gattggggag
     1201 gcgatgatga agcaggacca gaaggagcgt cccgcagagc caggggacca gaagccggac
     1261 gagaacccga aggacgcgga ctttacggag tgagcgtaag ggagtagggt aacgtcggtt
     1321 tggacacatc cggggagatt tcgggatgaa gctacatatc attggctgcg tcggagcgct
     1381 tgcgctcgcg gcagctgcct gcggcaagcc ggtgcccgcc cacgcgccct acgattccga
     1441 gcaggagcgg caggcggtgc tacggcggtt cgatccgttc tgcatggcgc gtgtgggcta
     1501 cgactaccgg ccctgcgccg ccgtcatccg cttccagcga ctcggcggcc acgagctcta
     1561 cgggctcgtg ctcggcgtcg acaggacgcg ggtgcatgtc cggtggggcc atgccttgcc
     1621 ggaccagttc gaccacgtgg cgctcgagac gaccgagatg ccgggcagga tcgtgcagat
     1681 cgagcgcctg gcgtcgccgg ccgggcagcg gctcctcgcg gaatacgagc ggggcagggt
     1741 gcctccgcct acgaagtagg gctggaggat ggggcggcgc gcgcgagcgt gccgccctga
     1801 acatttgacc tcggtgccgt taaatggcat acatgacccg ggtggccatc ccggcccgcg
     1861 aggagaaacg catggagctg ctggctctga atccgcacgg ggatctggag atcgcccggc
     1921 gccaagccca ggacctctgc gcggcattgc aggaggtctt cgacgagttc ccgatcggcg
     1981 tggccatgca cctggtgtgc gacgagctca ggaatgcggg cgagtccgag gtgtccttga
     2041 tcttgattga tgtgaccggc cctggcatgg aggactacgg cctgctgacc atcgatatgc
     2101 cctgcgggag caggctggag gagttccgat cctggggaat tcgggtacag taccagcgcc
     2161 cgcccgtgcg acttgaggca cacttcgtcg gccacgatgg cggcccgacg atacgagagt
     2221 tcatccgctg caagatcgcc atccaggaca tccttcggga ggatctcgaa cggcaacgcg
     2281 ttaaacgcag cagctgacac gggcatcgag gggcggccag gcgcacgagc gcggccgtcc
     2341 tttcactttt tatgcacagg atttggcgcg gaaaatgaag aggggtacta tctaggtata
     2401 tgtccaaaaa ctactacgac atcctcgggg tcgataagaa ggcaagcaag gaagagatca
     2461 agaaggcgtt ccggaagctc gcccagaagc accacccgga caagggcggg agcgaggaga
     2521 agttcaagga gatcaccgag gcgtacgcga ccttggcgga cgagaagcgc cgccgcgagt
     2581 acgactccta cggccagacc ttcggcgcgg gcggggccgg cggcttcgat ttctccggct
     2641 tccagaacgg ctccgtcgag ttcgatctct cggacctctt cgagggcttc ggcgacatct
     2701 tcggcacggg cacccgcggg ccgcgcatga ggcgcgggcg cgacatttct atagacatcg
     2761 aggtgtcttt caaggacgcc gtgctcggca cgaagcggca ggtgctcatc acgaaggtcg
     2821 ggaagtgcga catctgccag ggctcgggcg gcaagccggg gacgcagatg gagagctgca
     2881 ccacctgcaa cggggcaggc cgcgtgcacg agacgcgcaa ctcgcccttc gggaccttcg
     2941 cgagcgtgcg gagctgcccg acctgcgagg cgaccggcaa ggtgcccaag gagaagtgcg
     3001 gtacctgcgc gggccgcggc gtcctcaaga aggaggagga gctgaagatc gccattccag
     3061 caggcatcga cggcggggag atgatccgca tgccggggat gggcgaggcg gtgaagggcg
     3121 gggtctcggg cgacctctac gtgaaggtgc acgtgaagcc gcatccggtc ttcaagaagc
     3181 acggtctgaa cctcgtcatg gacctgccgg tgaaattgac cgatgccctg cttggcacta
     3241 ccgcctccgt cgagacgatc gagggcaaga agctcgaggt gaagatcccg ccgatggccg
     3301 cgcccgagga gacactgcgc atcaagggca agggcgtccg ggtggacgat gcgcaaggag
     3361 accttctcat caagctcacc gtcgtgctcc caaagaagct ctccgcgagg gccaagcgcg
     3421 catttgagga actgaaggga gagggcctct aggtgggagg tatacttgcg ctatggacgt
     3481 cacctccgta gctacccagg tctcggacct cgccacgagc ggggcagcga atgcctggct
     3541 gcttgccggg aacttcgtca tcctcctcgt gctcttcatc gtgatgctcc tgttctcgta
     3601 caaggcgggg cgcggtggga tcatctccct gatcgtcgca ttctatgccg ggtacgccat
     3661 ctacatggtc ttcccgtata cccagatggt ggtgaatgcc ggtggcacgc cgttggtcaa
     3721 ggcagtgatc agcgttgccg tcttcgcgat cgcgaccttc atcccgttcc atttcgtgca
     3781 gcggctcgcg agcgggggat tcggggtgct ctcggtggtg ccgcggttcg tgctctcctt
     3841 cctcgcggcg gcattcctgc ttgcgctcgc gtaccacgtc ttcgatatct cggatatcta
     3901 taccttcccg gcgcccatcg ataagctatt cgcgcccgag gggtatttct tctggtggtt
     3961 cattgcgccc atggccgggc tcctcttcct ggtgcattag gctgattatt cctagagtgt
     4021 aaagctcctg tgctatacta gcggcttatg tcagacgata cgaagcggga gatcgtcccc
     4081 gcggggcgca tcctgcgcgc ctacgcgccc gaactcggca agcatccggg ccttttcgtc
     4141 atggtcatgg tgggcatggc gggcatcgtc gcgaccgacc tcatcgcgcc gctctacctg
     4201 aagcagttct tcaatgccat cgcggggacc gacccgacca cgaacctggt ggctgagctg
     4261 tcgatgatca tcaccatcgt gatcgcgctc tatgtcgcca actgggtcgc cttccgcgcc
     4321 tactcgatcg ggctcatcaa cctggagctc aaagcgatga gccggctcta caatcacgcc
     4381 ttcgactacc tcatccgcca ttcccagcac ttcttcacct cgcagttcac gggcacgctc
     4441 acgcgccgcg tagcgaagtt ctcggacgcc ttcgagactc tcgtggatac ggtcttcatg
     4501 cagttcgttc cgaccgcgat attcgtcgtg ggcgccgtca tcgtgctcgc gacccagcac
     4561 ccctggctcg gcggcatcct cgcggtctgg tccgtgatct tcatcatcgt gcagatatgg
     4621 ctcgcgaacc tgcggcagcc cctccgcgag gcgcagagcc gcgccgagag cgagcggacc
     4681 ggcgcgaccg cggacgcgct ctcgaaccag agcgccatca cgcagttcgc tgcagaggag
     4741 gcggagtccc ggaagctcgc ggatgtcgtt gcgaggtggc ggcaggccgc gatgcgctcc
     4801 tggggcgccg atgagttcat ctgggccgtg ctcggcctcc ttatgatcgg catccaggcg
     4861 gcgctcctgt acgctgcggt cgagctctgg agtaggggac ttctgacggt gggcgacttc
     4921 gtcctcatcc aggcctacct catcacgacc ttcgaacgac tgatcggcat caaccgggac
     4981 ctgcggcgct tctacgcggg gctcgccaac gcctcagaga tgatcaccat cctcgatacg
     5041 gactacgaca tcaaggatgc ggcgggcgcc cgcgagctcg tcgtcgacga ggggtcgatc
     5101 acgttcgagg atgtctcctt cggctaccag gaccggccca cgcttgcagg cttctcgctt
     5161 tcgataccga gccaccagaa ggtcgcgctc gtcggtcctt cgggagcggg gaagtccacc
     5221 atcacgaaac ttctgctccg tctctatgac gtaacaggcg gcaccatccg catcgatggg
     5281 caggatatac gggaagtaac gcaggagagc cttcggaagg ccatctcctt cgtgccccag
     5341 gagccggcac tcttccaccg cagcctccgg gagaatatct cctacgggag gccggacgcc
     5401 tcggaagcgg agatcatcga ggcagcgaag aaggcgcatt gccatgagtt tatcgccgga
     5461 cttccgctcg ggtacgagac gtacgtgggg gagcggggcg tgaagctctc gggcggggag
     5521 cgccagcggg tggcgatcgc acgcgccatc ctgaaggacg cacccatcct cgtgctcgac
     5581 gaggccacct cctcgctcga ttcggagtcc gagtccttga tccaggacgc gctccggacg
     5641 ctcatggcgg ggaagacggt ggtggtgatc gcgcaccggc tctctaccat catgagcatg
     5701 gaccgcatcg tggtgctcga gggcggccgg gtggtcgccg acggtacgca tgacgagctc
     5761 gtggcggatg cgggcggcct ctaccacaag ctctggagca tccaggccgg cggattcatc
     5821 gcaggctagc tcggctatac tctatccatg cctccctacc tctccgggat cctcgtgggc
     5881 ttcctcgagc ccgtgctcca tgcctggagc aacatcatcg acaaccattt ctcgaacgcg
     5941 gtgttcccgc ggctgccggt gctgcttttc ttcggggtgg cgctcaatat cgtcttcctg
     6001 ccgatcacgt tcctcgcggg gcttcccgtg atgctgccgg tcacgctctt gggcatcgtc
     6061 ttcgtgatcg cgatcgtgaa cgtcgtctac gccttcccgt actacctcgc gctccgggat
     6121 gcggacacct cggtcgtggc gtccctgttc tcgctcggga ggatcttcgt gccggtcttg
     6181 gcgtacttca tcttgggcga ggtgctcacc ctgccgcagt acgccgggtt cttcgtgatc
     6241 atcctctcgt cggcgctcct gaccctcgat ctgaagaagc tgaaggtcaa ccgggccttc
     6301 ttcctcatgc tcgcctgctc cttcgtgctc gcgttccaga ccatcctcta caaattcgtc
     6361 ttcgacgcgg gcgcctcctg gccgagcgtg gtcgcggcct tgaccgtctt cgagctcgtg
     6421 atcgcgggcg cgatcatgtt cgccacgacc tcgctccggc acatccggga ggacctcggg
     6481 accgtccgga ggaactggaa gctcttcgtc gcgcagcagg gccttgagtg gggcgggaac
     6541 gtcggcgcct cctacgccat ctccattctc caggtgacgg tcgcgcaggc gatcgcgagc
     6601 acgcagccct tcttcgtgct cctctacgcg gtcctcttca agaagtggtt cccgaacgcg
     6661 ttcaacgaaa ccacggcacg gggcgatctt ctcaagaaag gcatcttgtt cgcatgcatc
     6721 atccttggga ccgtgctcgt ggttgcgcac gggcccgtca ttgacgtgta gggaagtggg
     6781 ctatactgaa gctaagtatg ctgatcggcg aacggctcat gtacggtgcg atgatcgcga
     6841 tggtcgtggg cgcgatcagc tggaccgcct ggctctacct cggggagaag gagtatccgt
     6901 tcatcgtgga ggcggcctgc gacccgagcg tcgagacctg cttccaccgc gcctgctcgg
     6961 acacctcgaa cacgggagac tgcccgccca acgcgctcga ggactacaag at
//
LOCUS       DASPKG010000004         4339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1363334, whole genome shotgun sequence.
ACCESSION   DASPKG010000004 DASPKG010000000
VERSION     DASPKG010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363334"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VEA36_00150"
     CDS             complement(<1..406)
                     /locus_tag="VEA36_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HYF28764.1"
                     /translation="MPKVFIASDHAGFELKQELVPYIGTLGGYDPCDIGAFELDPDDD
                     YPTYILPCARRVAGEPGSLGIVLGASGQGEAMAANRIEGIRCAVFYGAPRGTQTDAGG
                     NELDLVASVRAHNDANMLSLGARFITLDEARAA"
     gene            complement(399..1409)
                     /locus_tag="VEA36_00155"
     CDS             complement(399..1409)
                     /locus_tag="VEA36_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015185359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HYF28765.1"
                     /translation="MATRIAINGFGRIGRAFVRQAWNRPEIEIVAVNDLGSLENLAYL
                     LKYDTVYKKAPFPVEAKDGKLMIDHKEVTFLSEKEPQHLPWKKLGVDVVIESTGFFTD
                     YMKAQAHIIAGARRVVISAPVKEDNPLGATILMGLDEGGLKKAQITSNASCTTNSASP
                     VIAILDEAIGIEKAVLSTTHAYTASQSIVDGPAKKDMKEGRAAAQNIVPTSTGAAIAV
                     TKAYPSLAGKFDGISLRVPVPAGSIADVTFIAKRNTSAEEVNDILRKASVEARWQGIF
                     TTTDEELVSSDIVGEEFASIADLGMTRVVDGNLVKVLAWYDNEAGYTHTLVEHAIKAG
                     HA"
     gene            complement(1411..2751)
                     /locus_tag="VEA36_00160"
     CDS             complement(1411..2751)
                     /locus_tag="VEA36_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28766.1"
                     /translation="MNIAVRALAFVLVFSLLIPVHASAMVTPILAENTSSATLSSTLE
                     RWIPLSANGGNARSASDTNRQVFPISGTLKNLRVHIGDALSAGTSYTITVYKNGVATG
                     LTCTVTDAALTCNDLVNTVSIAAGDFASVRILPTNSPVGSDELRIGVAFEGGTPGETF
                     ILGWGSGANATINSYASPGWATSFTTPEFSRASVFPTSGTIDNLHVYLRSTAPGAGRS
                     WTWYLRKGTSINTMATTSLTCQIADTATTCTDPTNSVSVSAGDVLSFVAVPSGTPSNT
                     THSYSFRFRPTIDGESVMMAHTNNSLSVGVTRYIPTGGLVLNGAQGTNATGTMAVAPV
                     PFTWRKLYTDFNTAPGGVTSRTISANLNEVVQSLSCAVSGTNTTCNDLNNSISVAAGD
                     LITWQSTTSGSPAASANIRVSGVMFIDPSTVTYGQPRFFNLGTMLLRAGTLIFR"
     gene            complement(2779..>4339)
                     /locus_tag="VEA36_00165"
     CDS             complement(2779..>4339)
                     /locus_tag="VEA36_00165"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HYF28767.1"
                     /translation="YLALTDTDAATNSKHLILSNVDGIFRIGTSTDDFSSTSTAFQID
                     PRGSAVFGLGTTSPWRSLSVVGTVAINGLSAVSGTDQSVCIDGTTMELTTQSGDTCAV
                     SAARYKNDITPLELNALDLINQLVPVSYTYKADESGTLYWGLTADNAASTSAQLAYFN
                     PDGSVQTLNTFGFISLFTKSLQELNQELFIGTSSLSLRVDALEDRFGIDNDGSDDEEG
                     EAAPAAPTTLTLTGDARAARFIATVSETSFTVGSSTITATIPSELLTEGGMDIGKLAT
                     YTLANLDDVRTRLDLATERLDTIEVRLAELENASSTPSIIPESIFGSMLASAMAGFEA
                     TIENGLAYFKRIKIGEIVIAKEDKTSAVGSGVILAGNTVVEVKNPMATTTSKIIITPT
                     SEIDGSWYISDKDEGSFRMRLSKAQDHDFTFDYLIIQTEGDDETEQDPQEVEVIEVSE
                     PTSEPAPAPAPAPAPEPTPTPEPAPAPAPAPAPEPTPTPEPEPAPAPAPAPAPEPEPA
                     PPAAEGGETTP"
BASE COUNT          780 a         1231 c         1450 g          878 t
ORIGIN      
        1 cggccgccct tgcctcgtcg agcgtgataa agcgggcgcc gagcgagagc atgttggcat
       61 cgttgtgggc gcgcaccgag gcgacgagat cgagctcgtt cccgcccgcg tccgtctgcg
      121 tgccccgcgg cgcgccgtag aacacggcgc agcggatgcc ctcgatccgg ttggcggcca
      181 tggcttcccc ctgccccgag gccccgagga cgatgccgag cgatcccggc tctcccgcca
      241 cccgccttgc gcagggcagg atgtaggtcg ggtagtcgtc gtcggggtcg agctcgaacg
      301 ccccgatgtc gcagggatcg tagcccccga gcgtaccgat atagggtacg agctcctgct
      361 tcagctcgaa tcccgcgtga tcgctggcga tgaagacctt aggcatggcc ggctttgatg
      421 gcgtgctcga cgagcgtgtg cgtgtatccc gcctcgttgt cgtaccaggc gagcaccttc
      481 acgaggttcc cgtccacgac ccgcgtcatc ccgaggtccg cgatcgaggc aaactcttcc
      541 cccacgatgt cgcttgagac gagctcctcg tcggtcgtgg tgaagatgcc ctgccagcgc
      601 gcctccaccg atgccttcct gaggatgtcg ttcacttctt ctgcggaggt attccgcttt
      661 gcgatgaagg tgacgtccgc gatggagcct gcgggtaccg gtacgcgaag cgagatgccg
      721 tcgaacttgc cggccaggga cgggtacgcc ttcgtgaccg cgatcgcggc accggtcgag
      781 gtcggcacga tgttctgcgc cgccgccctc ccctccttca tgtccttctt tgcgggaccg
      841 tcgacgatcg actggcttgc ggtgtaggcg tgggtggtcg agagaaccgc cttctcgatg
      901 ccgatggcct cgtcgaggat ggcgatcacg gggctcgcag agttcgtggt gcaggatgca
      961 ttgctcgtga tctgcgcctt cttgaggcct ccctcatcga ggcccatgag gatggtggcg
     1021 ccaagcggat tgtcctcctt cacgggggcg gagataacga cccggcgagc tccggcaatg
     1081 atatgcgcct gggctttcat gtagtcggtg aagaagccgg tggactcgat gacgacatcc
     1141 accccgagct tcttccaggg gaggtgctgc ggctccttct ccgagaggaa ggtgacttcc
     1201 ttgtggtcga tcatgagctt gccgtccttg gcctcgactg gaaacggcgc cttcttatat
     1261 acggtgtcgt acttgagcag gtacgcgagg ttctcgaggg agccgaggtc gttcacggcc
     1321 acgatctcaa tttcaggacg gttccatgcc tggcgtacga aggcccggcc gatgcgcccg
     1381 aacccgttga tcgcgatgcg cgtagccatg ttagcggaag atgagggtgc cggcccgcaa
     1441 gagcatagtg ccgagattga agaagcgggg ctggccgtag gttacggtac tcgggtcgat
     1501 gaacatgacc ccgctgacgc ggatattagc ggaagcagcg ggagagcccg aggtcgtgga
     1561 ttgccaggtt atcaggtcgc ctgcggccac cgatatggaa ttattgaggt cattgcaggt
     1621 cgtgttcgtg ccgcttaccg cacagctgag agattggaca acctcattca aattcgcgct
     1681 tatggtacgg ctcgtaacgc cacctggcgc cgtgttgaaa tcggtataga gctttctcca
     1741 ggtgaacgga acgggcgcca ccgccatcgt gccggtggcg ttcgtaccct gtgcgccgtt
     1801 gaggaccaaa ccgccggtgg gaatataccg ggtgacaccg acgctcaggc tgttgttggt
     1861 gtgagccatc atgacgctct ctccgtctat ggttggtcta aaccggaacg agtacgaatg
     1921 agtggtgttg gacggggtgc cgcttgggac ggcgacgaat gaaagaacat cgcctgcgct
     1981 cactgatacg gaattcgtag ggtcagtaca ggtggtagcg gtgtctgcaa tttggcacgt
     2041 cagcgacgtc gtcgccattg tgttgatact tgtacccttg cgcaggtacc acgtccatga
     2101 cctacccgca ccaggtgccg tggagcgaag atacacgtga aggttatcaa tcgtgcccga
     2161 ggtcggaaat acggaggcac gtgagaactc gggagtcgta aatgaggtcg cccaaccggg
     2221 agacgcatag ctgttgatag tggcgtttgc accggatccc cacccgagaa taaacgtctc
     2281 gcccggggtg cctccctcaa acgcgacgcc gatgcgtaac tcatctgacc caaccggtga
     2341 gtttgtcggg agaatgcgta ccgatgcgaa gtcgccggcg gcaattgaga cggtatttac
     2401 cagatcatta cacgtaagtg ccgcgtcggt aacggtgcag gtaagacccg tagcaacgcc
     2461 gtttttgtag acggtaatgg tgtacgaggt tcctgcggag agtgcgtcac ctatgtgtac
     2521 acggaggttt ttgagcgtcc cgctaatcgg aaacacctgc cggttcgtat cagaggcgga
     2581 acgcgcatta ccgccattgg cggaaagcgg gatccaacgt tcaagggtcg agctgagggt
     2641 ggcagatgag gtgttctccg caagaatggg ggtcaccatc gcggacgcgt gaaccggtat
     2701 aaggagcgag aacacgagca caaatgcgag agcgcggacg gcaatgttca tagaatagtg
     2761 attagtataa cagtgcggct atggagtggt ttccccaccc tccgctgccg gcggtgcggg
     2821 ttccggttca ggcgctggtg ccggtgccgg tgctggagca ggttccggtt caggcgttgg
     2881 cgttggttca ggggccggtg cgggagcagg agcaggagca ggttctggcg ttggcgttgg
     2941 ttcaggggcc ggtgcgggag cgggagcggg agcgggttcg gaagtcggct ccgacacctc
     3001 gatcacctcc acctcctgcg gatcctgctc ggtctcgtca tccccttccg tctggatgat
     3061 gaggtagtcg aaggtgaagt catggtcctg tgccttggag aggcgcatgc ggaagcttcc
     3121 ctcgtccttg tcggagatat accaggaacc gtcgatctcc gaagtcggcg taatgatgat
     3181 cttgctcgtc gtggtcgcca tcgggttctt cacctcgacc accgtattgc ccgcgaggat
     3241 gacgcccgag cccaccgcgg acgtcttgtc ctccttcgcg atcacgatct cgccgatctt
     3301 gatgcgcttg aagtacgcga gcccgttctc gatggtcgct tcgaacccag ccatcgcgct
     3361 cgcaagcatg ctgccgaaga tcgattccgg aatgatgctt ggagtggagg aggcgttctc
     3421 gagctcagcg agccgtacct cgatcgtatc gaggcgctcg gtcgcaaggt cgaggcgggt
     3481 gcgcacgtca tcgaggttcg cgagcgtgta cgttgcaagc ttcccaatat ccatgccgcc
     3541 ctcggtcagg agctcgctcg ggatggtggc ggtgatcgtc gaggatccga cggtgaacga
     3601 ggtctcggat accgtggcga tgaaccgcgc cgcacgcgca tctcccgtga gcgtgagcgt
     3661 cgtcggtgcc gcgggcgccg cttcgccctc ctcgtcgtcg gatccatcgt tatcgatgcc
     3721 gaagcgatcc tcaagcgcat cgacccggag cgagagcgag gaggtaccga tgaagagctc
     3781 ctgattgagt tcctgcagcg acttcgtgaa gagcgaaatg aaaccgaagg tgttcagggt
     3841 ctggaccgag ccgtccggat tgaagtacgc gagctgcgcg gaggtcgatg cggcgttgtc
     3901 tgcggtgagg ccccagtaga gcgtgccgga ctcgtccgcc ttataggtgt aggagaccgg
     3961 taccagctga ttaatgaggt cgagcgcgtt caattcgagc ggggtgatgt cgttcttgta
     4021 gcgggccgcc gagaccgcgc aggtgtcgcc ggattgcgtg gtgagctcca tcgtcgtgcc
     4081 gtcgatacag accgactggt cggtgcccga gaccgccgag agcccattga tcgcgaccgt
     4141 acccacgacc gagagcgagc gccaggggct cgtggtgccg aggccgaaga ccgccgatcc
     4201 ccgcggatcg atctggaagg cggtcgaggt tgacgagaag tcgtccgtcg acgtcccgat
     4261 gcggaagatg ccgtccacgt tcgagaggat gaggtgcttg ctgttggtcg cagcgtccgt
     4321 atcggtaagc gcgaggtag
//
LOCUS       DASPKG010000005         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_957448, whole genome shotgun sequence.
ACCESSION   DASPKG010000005 DASPKG010000000
VERSION     DASPKG010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957448"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="VEA36_00170"
     CDS             complement(<1..1533)
                     /locus_tag="VEA36_00170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HYF28768.1"
                     /translation="MSPSPAPAPAPAPRGDRGGQPSPARGPRRARGGRVSGGAGRPGR
                     VSRQIRRTLRRPTGPSKSEGAPKNTPDYYPEKPAQNVVRVVPLGGVEEVGRNMFSVET
                     NGDIFVFDVGFQFTSEDTSPGVDYMLPNTKYLEKNQDRVRAVFITHGHLDHIGGVPFL
                     MHRFGNPPIYTMQLSATMIAKRQEEYPDMPKPNLVVVEGGQTHTIGSTRVHFFPVTHS
                     IPDSMGIKVETPYGNVIVTGDLKVEHVDNIPVPEEEQKWSAIGREKNLLFIADSTSAE
                     LEGFSVPEQKVRDTLENIIKTVKGRLIIGTFASQFDRMVNIIRLAEKYGKKIVMEGRS
                     IKTNLEIAQKLGILTLDKGTLISAQEIGDYPPDKIVILATGAQGEEFAALMRIATKQH
                     KNITFTERDTVVLSSSVIPGNEISVQKLKDNLLHNHVTLLHYRSADVHSGGHARAAEL
                     VWINQKVNAKFFMPAYGYYSMTASHAKAVEQAGRPRESIILSENGSVIDILDGEKLEI
                     HKQ"
     gene            complement(1611..>2327)
                     /locus_tag="VEA36_00175"
     CDS             complement(1611..>2327)
                     /locus_tag="VEA36_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYF28769.1"
                     /translation="NIVEGVLGGYGTPLMGPLPPGSGIESPALPDPATRIETARLILD
                     EAGWDYEEGAGWEKDGTVMPTITIRTSSVAELKLIAAAVQADWEALGVPVEVELYEPG
                     SLTQEVIRPRDYEALLFGEVIGRDRDLYAFWHSGQRKDPGLNIAVYANETVDALLSRI
                     RTESDEEAKRADLARVSGLIAEDYPAAFTHAPDFLYAVPQDVRGIVLPQIAAPADRFA
                     TVATWHRYTEFVWPLLVSSK"
BASE COUNT          364 a          707 c          805 g          451 t
ORIGIN      
        1 ctgcttgtgg atctcgagct tctcgccatc gaggatatca atgaccgagc cgttctcgga
       61 taggatgatg gactccctcg gcctgccggc ctgttcgacc gctttagcgt gggaggcggt
      121 catcgagtag tacccgtagg cgggcatgaa gaacttggcg ttcaccttct ggttgatcca
      181 gacgagctcg gccgcgcgcg cgtggccgcc cgagtgcacg tccgccgaac ggtagtggag
      241 gagcgtgacg tggttatgta ggaggttgtc cttcagcttc tggaccgaga tctcgttccc
      301 ggggatgacg gaggaggaga gcaccacggt atcgcgctcg gtgaacgtga tgttcttgtg
      361 ctgcttggtc gcgatgcgca tgagcgcggc gaactcctcg ccctgcgcgc cggtcgcgag
      421 gatcacgatc ttgtccggcg ggtagtcgcc gatctcctgc gcgctgatca gggtgccctt
      481 atcgagcgtg aggatgccga gcttctgcgc gatctcgagg ttcgtcttga tggagcggcc
      541 ctccatgacg atcttcttcc cgtacttctc cgccagccgg atgatgttga ccatgcggtc
      601 gaactgggag gcgaaggtgc cgatgatgag gcggcccttc acggtcttga tgatgttctc
      661 gagcgtgtcg cgcaccttct gctcggggac cgagaagccc tcgagctcgg cgctcgtgga
      721 gtccgcgatg aagaggaggt tcttctcccg gccgatcgcg ctccatttct gctcttcctc
      781 ggggaccggg atgttgtcca cgtgctcgac cttgaggtct cccgtgacga tgacgttgcc
      841 gtagggcgtc tccaccttga tgcccatgga atccgggatg gagtgggtga cggggaagaa
      901 gtgcacccgc gtcgacccga tggtgtgcgt ctggccgccc tcgaccacga cgaggttcgg
      961 ctttggcata tccgggtact cctcctggcg cttggcgatc atcgtcgccg agagctgcat
     1021 ggtgtagatc ggcggattgc cgaagcggtg catgaggaag gggactccgc cgatgtggtc
     1081 gaggtgcccg tgcgtgatga agaccgcccg gacgcggtcc tggttcttct cgaggtactt
     1141 cgtgttcggg agcatgtagt cgacgcccgg ggaggtgtcc tcagaggtga actggaagcc
     1201 cacgtcgaag acgaatatgt cgccattggt ttcgacgctg aacatgttgc ggcctacctc
     1261 ctcgacgcca ccaagcggca cgacgcgcac gacgttttgt gccggcttct cggggtagta
     1321 atcgggggtg ttcttcgggg cgccctcgct tttggaggga ccggtcggac gccggagcgt
     1381 ccggcggatc tgccgcgaga cgcggcctgg acggccggcg cctccggaaa cgcgtccgcc
     1441 gcgcgcgcgg cgcgggccgc gggcggggga gggctggccg ccgcgatcgc cacgaggggc
     1501 gggtgcgggg gccggagcgg gtgatggaga catactgatt gaatcttgaa tgttagtaag
     1561 cgattcctac caagtagaga gcagtaggca tgcagtatct gatgaaccta ctacttgcta
     1621 ctcactagaa gcggccacac gaactccgtg taccgatgcc aggtcgcgac cgttgcgaag
     1681 cgatccgccg gtgccgcgat ctggggaagc acgatcccgc gcacgtcttg gggtacagca
     1741 tacaggaagt ccggtgcgtg cgtaaaggcc gcggggtaat cctccgcgat gagcccggag
     1801 acccgggcga ggtccgcccg cttcgcctcc tcgtcgctct cggtccggat ccgggacagc
     1861 agcgcatcca cggtctcgtt cgcgtagacc gcgatgttga ggcccgggtc cttgcgctgc
     1921 cccgaatgcc agaaggcgta gaggtcgcgg tcccggccga tcacctcgcc gaagaggagc
     1981 gcctcgtagt cccggggccg gatgacctcc tgggtgaggc ttccgggctc atagagctcg
     2041 acctcgaccg gcacgccaag cgcctcccaa tccgcctgca cggcggcggc gatcaatttc
     2101 agctccgcaa ccgacgacgt gcggatggtg atggtgggca tcactgtccc gtccttctcc
     2161 cagcccgcgc cctcctcgta gtcccagccg gcctcgtcga ggatgaggcg cgccgtctcg
     2221 atgcgcgtcg cgggatccgg gagcgctggg gactcgatgc cggacccggg cggcaaggga
     2281 cccatgagcg gggtgccgta cccgccgagg acgccctcca cgatgtt
//
LOCUS       DASPKG010000006        10247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_822412, whole genome shotgun sequence.
ACCESSION   DASPKG010000006 DASPKG010000000
VERSION     DASPKG010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10247)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10247
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822412"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(424..846)
                     /locus_tag="VEA36_00180"
     CDS             complement(424..846)
                     /locus_tag="VEA36_00180"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYF28770.1"
                     /translation="MRNVLHCTCGSKFGALAALFLRVVVGVIFLVHGWQKLQNGIPGT
                     AGFLATLGFPAPEFFAVLLIAAEVIGGALLIIGLYTHWAAKILAIVALVALVTVHLGK
                     GFSVATGGYEFILLILAATLSVMAMGGGKWSVDAHLKK"
     gene            complement(924..1547)
                     /locus_tag="VEA36_00185"
     CDS             complement(924..1547)
                     /locus_tag="VEA36_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28771.1"
                     /translation="MNRSKLLLGGLVLLFLIGVAVALSQLGDAGNREVVNQYRSAVFG
                     ITYPLGLEIEEYPNGSLSIGAPATGGFDSAVDLTVVTPADGTTLEDFDTYLRAQVQGL
                     CAADAPSEQVSCATIERERTVRNAKGETAKEYQLTLTRTRAGQEPQTLSFGPIYAYVL
                     PPIDEDAPNRYSALLVHAPVPDVLNGNPDLELLGRINDSLTVGHSRR"
     gene            complement(1606..1953)
                     /locus_tag="VEA36_00190"
     CDS             complement(1606..1953)
                     /locus_tag="VEA36_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28772.1"
                     /translation="MHKLIWIGAFAVIALAAFSFVPPPARPQPEAAPPPALSGAERDS
                     VAENYVRTNIAALSPEPAVLGGTYYVTAVHAAAGTGVVSYEDGHMAYTADFTYTADAG
                     GRNITVTSFVIRE"
     gene            2042..2338
                     /locus_tag="VEA36_00195"
     CDS             2042..2338
                     /locus_tag="VEA36_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28773.1"
                     /translation="MAYILYFLAGLIFANGVPHFVKGIMGERHDTPLKGPSGAMLNVV
                     WGTANLFVAGWLWLYASIMPQALGFALVAFFLGILVAGITLAKRFEADRREAQF"
     gene            complement(2341..2859)
                     /locus_tag="VEA36_00200"
     CDS             complement(2341..2859)
                     /locus_tag="VEA36_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28774.1"
                     /translation="MKAFLIAALLLLAAPAAAHHPDGACTMIYQPVCGAKEVQCVRAP
                     CYPQYQTYGNACVMRNDHATLVHEGECTAAEAGPVIPTPSYQPPAHCSAWFDGCNHCS
                     RQSDGTAVCTEKACIGPMPAGYCTTYDAPAPQVPTATEEPVATAEPVAEEAPKGFLSA
                     LWERILALFAWF"
     gene            complement(2894..3277)
                     /locus_tag="VEA36_00205"
     CDS             complement(2894..3277)
                     /locus_tag="VEA36_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28775.1"
                     /translation="MEIFLAKLLGLYFIIMGVMSLLRRKSIIPAIREFASNTGLRFVI
                     AAIELVAGLAIVIAYPQVSVSLAGIISLVGYMMVIESLVYMAMPAKFVQHFIRSFNKP
                     MWYHAGGALAIVAGIYLAGTAFGYF"
     gene            complement(3347..3772)
                     /locus_tag="VEA36_00210"
     CDS             complement(3347..3772)
                     /locus_tag="VEA36_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HYF28776.1"
                     /translation="MIEGIIGPVELQFLVAMAFALLLGFLIGAEREARGKDAGISTHV
                     LVIAGAALFTQISAIVDPMSTSRIAAQIVSGIGFLGAGLILKEGASVKNLTTAASLWV
                     AGAIGMAIGFGFYVLAVIAAVVSILAPRIPHFHRRSAAE"
     gene            complement(3788..4276)
                     /locus_tag="VEA36_00215"
     CDS             complement(3788..4276)
                     /locus_tag="VEA36_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28777.1"
                     /translation="MKKNTWLWIIGISALLALIWFASASTREGTATKRTNREIAMSCM
                     THESDTFHIHPTLSIMVNGEPIVVPADIGITPGCMRAIHTHTPDGIIHIESNEPRDFT
                     LADFFANWEKPFSATQILEHVADDTHRIRMTVNGAEVDTFENTILRDKDQIVIYYEPV
                     TP"
     gene            complement(4299..4676)
                     /locus_tag="VEA36_00220"
     CDS             complement(4299..4676)
                     /locus_tag="VEA36_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015494379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYF28778.1"
                     /translation="MKFDAVEVTSTNIPKTIQFYSLLGFSFPEYTGQQHVDAVTEAGG
                     VRFMIDDATMMQGILGYAPKPGNHASIAINCGTPDQVDATVAAVKEAGFAIAKEPWDA
                     FWGQRYAVVEDPDGYLVDLYASR"
     gene            complement(4687..5214)
                     /locus_tag="VEA36_00225"
     CDS             complement(4687..5214)
                     /locus_tag="VEA36_00225"
                     /inference="COORDINATES: protein motif:HMM:NF019440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HYF28779.1"
                     /translation="MDPRSNETAGYHLFLEPTGALNERLQETIRTLAAAYDGPIFPPH
                     VTILANLEGNEEDILSHAQMLAQQLPPLTVMLGELGGEPSFFRAFYLTIKEDPELMSA
                     HEVAQQTFGTPAEPYRPHLSLYYGNIDDGVREAMRSAGEYPSGESFRCDRLALYRTVG
                     PADQWLRIGEVPFTR"
     gene            complement(5214..6125)
                     /locus_tag="VEA36_00230"
     CDS             complement(5214..6125)
                     /locus_tag="VEA36_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYF28780.1"
                     /translation="MQKIVPNLWFDTEGEEAAKLYTSLFSDSRITQVTHYPNAGQEIT
                     GKEPGSVMTVAFELAGQSFIALNGGPHFTLNPSISFTVACKTKEEVDRLWSTLSEGGN
                     ILMPLDAYPFSERYGWCSDRYGLSWQLVLDDGHYPYKNRIATSFLFVGKVAGKAEEAI
                     TLYASIFPESKLGDIARWPAGMEPEIAGTVMHGAVTLAGQEFFAMDSANEGHTFSFNE
                     AFSLLVNCRDQAEVDEYTARLTEGGGEQGPCGWVKDRFGVSWQIAPIILSEMLKDPDT
                     AKQDRVMSAMLKMQKIDIAALEAAYRG"
     gene            complement(6206..6553)
                     /locus_tag="VEA36_00235"
     CDS             complement(6206..6553)
                     /locus_tag="VEA36_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HYF28781.1"
                     /translation="MADRMTFEEFQAFIDAQDALFKSVNKGGQGTEAIFARTIKLGEE
                     YGELCDEVLAAAGDQRRDKMRAERDLEGEFADVVIVTFLLAKAMGVDMPEALRQKIEK
                     IKEKHNPQIGGRP"
     gene            complement(6557..7012)
                     /locus_tag="VEA36_00240"
     CDS             complement(6557..7012)
                     /locus_tag="VEA36_00240"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYF28782.1"
                     /translation="MGERHDKRAREILSKIRYATVATVTSDGKSWSSPIAPVIDTDFN
                     IYWFCDKDSQHARNIHTNPRIVVVVHDLADPGSERDRVYMEADVIELTDPTEIQRVRN
                     ANRMGSPPDLEEFAGHAVRKCYKSIPRKVWMNDPEGGSGAHGYRIDIHL"
     gene            complement(7053..7631)
                     /locus_tag="VEA36_00245"
     CDS             complement(7053..7631)
                     /locus_tag="VEA36_00245"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYF28783.1"
                     /translation="MRKVILVHGWDGTPNTGWFPWLQRELEARGFVVVAPQLPDTDAP
                     RIATWVPALAEAIGTVGEHMYLVGHSMGCQAILRYLEALPAGQKVGGAIFVAGFFRPL
                     TGLETDEEREIDAEWANTPIDTDKVRSHLPESFALFSDDDPWVPAENAKDFRDRLGSE
                     TLIQHGQRHFNEGAGFTTLPIVLEKLLEMAAS"
     gene            complement(7628..8161)
                     /locus_tag="VEA36_00250"
     CDS             complement(7628..8161)
                     /locus_tag="VEA36_00250"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYF28784.1"
                     /translation="MNCTEHSPSLSNAPSLSGQPSSRRNMNALTCGAYCKNDMPDEVH
                     DFLDQQRVGVISVEMPNGAPHGSTVHFAYQRDPLTFVFLTDRTYRKVEPMQGGASTRA
                     SFVVGTDESQMRTLQLDGTARLTDSELHWEAYFSKFPEKREQYGDPDDVFFVFTPTWW
                     RYTNYQVPEGKQVIESL"
     gene            complement(8049..8744)
                     /locus_tag="VEA36_00255"
     CDS             complement(8049..8744)
                     /locus_tag="VEA36_00255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYF28785.1"
                     /translation="MKEFTHQDQQRIWDEEHKNPLVLKQMDSQDVSSGVLAFYDFLQS
                     QGVNQGRGLEMGCGKGRNVIWLAQKGYEMNGFDFSPAAITEARKRASAAGVNPTFEVQ
                     DATTPWSYESDYFDFGIDCFASTDIESPEGRAHAVKEMHRVLKPSGYLLAYLLSSEDE
                     YHKDINLNSPGPEKGSFLHPKTGKFEKTFDEQEIDELYGAFTLIEQRTLVKRTTFFEK
                     EYECINLWSVLQK"
     gene            complement(8791..9333)
                     /locus_tag="VEA36_00260"
     CDS             complement(8791..9333)
                     /locus_tag="VEA36_00260"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYF28786.1"
                     /translation="MERVTHRGKIIEVVQKEVEQGGKPRIFEFARRSPGTRLIIPKDG
                     RILLTKEFRHELNGYDFRLPGGKVYDSLDEYNAALGSDADLEEAAKRAAIKEAREEAG
                     ITSKKLSLLHKSVCGATVVWDLFYFLVEDFEEGDQHLEEGEDIVREFFDLDTVRHMCL
                     DGSISEERSALVLLRYLNTV"
     gene            complement(9348..9893)
                     /locus_tag="VEA36_00265"
     CDS             complement(9348..9893)
                     /locus_tag="VEA36_00265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYF28787.1"
                     /translation="MVNITYFVHGTTIDNENGISSGWSDAPLSELGVQQSIALGEQTK
                     DKHFDVVFCSDLERAHKSAELAWGGRYQIIPDARLRECNYGDMNGHPSEAVEPLQESE
                     MHYRPFPNGESYEDVKNRVADFLKFLKENYDGKSVALVGHKAPQLSLDVLLKGMSWDE
                     ALANDWRKTKSWKPGWEYMLK"
     gene            complement(9912..9982)
                     /locus_tag="VEA36_00270"
     tRNA            complement(9912..9982)
                     /locus_tag="VEA36_00270"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9948..9950),aa:Gln,seq:ctg)
BASE COUNT         2018 a         3038 c         3105 g         2086 t
ORIGIN      
        1 aaatataggg tcggaaataa atgggaaaag cgaacgattt actcgacctg cactaattct
       61 caagaaactc tcccatagtt tctatctggt ggcgccgaca accacaagaa gacaggaggg
      121 tagctggtat gtgcatatta gatttggcac taccgatgag tacatatgct tgaatcatgt
      181 ccgcactatt gattaccggc gcctacattc aaaattggga caaattgata cagacgattt
      241 cagcaaagtg cgggaaggat tcagacgtct ttatatataa atgttccgcc gcccttgcgg
      301 gcggcggtcg cggctagtgc cgaatgtatt actaagtata cgcactcaat ttgtagagtc
      361 aatgaacgag gtttgcatcg aaaaagctcc ccgtaagagg agcttttcct tgcacgagag
      421 cacttatttc ttcaggtgcg catccaccga ccacttgcct ccgcccatcg ccatgaccga
      481 gagggtcgcg gcgaggatga gaagtatgaa ctcgtagccg ccggtcgcga ccgagaagcc
      541 cttcccgagg tgcacggtca cgagtgcgac cagggcgacg atcgcaagga tcttcgctgc
      601 ccagtgcgtg tagagaccga tgatgaggag cgcaccgccg atgacctcgg ccgcgatgag
      661 gaggacggcg aagaattccg gtgccgggaa gccgagcgtt gcgaggaagc ccgcggtgcc
      721 ggggatgccg ttctgaagct tctgccaccc gtgaacgagg aagatgacgc cgaccaccac
      781 cctaaggaag agggcggcga gcgcaccgaa cttcgaaccg caggtgcaat gcaatacgtt
      841 acgcatgtct gttattcggt actagggata acatacgtat tgtacccctg cccgcggccc
      901 ccggaccccg ggatgtggat gagctagcgc cgcgagtgcc ccaccgtgag cgaatcgttg
      961 atgcgcccga ggagctcgag gtccgggttc ccgttgagga cgtccggcac cggcgcatgc
     1021 acgaggagcg ccgagtaccg gttcggagca tcctcatcga tcggcgggag gacatatgcg
     1081 tagatgggcc cgaaggacag ggtctgcggc tcctgccccg cacgcgtgcg cgtgagcgtg
     1141 agctggtact ccttcgcggt ctcccccttc gcgttcctga cggtgcgctc gcgctcgatg
     1201 gtcgcgcagg agacctgctc gctcggcgcg tccgcggcgc acagcccctg cacctgcgcg
     1261 cgcaggtagg tatcaaaatc ctcgagcgtg gtgccgtctg cgggcgtcac gacggtgagg
     1321 tccacggcgg aatcgaagcc gccagtcgcg ggcgctccga tcgagagcga gccgttcggg
     1381 tactcctcga tctcgaggcc cagggggtag gtgatcccga agaccgcgct gcggtactgg
     1441 ttcaccactt cgcgattgcc cgcgtccccg agctggctga gggcgaccgc cacgccgatg
     1501 aggaaaagga gcacgagtcc gccgaggagg agcttggatc tattcatggg cataatctgg
     1561 cgaagcggag gttaacggtt aatgaaacat cggggtacgg ccgacttact ccctgatgac
     1621 gaacgaggtg acggttatgt tacgccctcc cgcgtccgcc gtataggtga aatccgcggt
     1681 gtaggccatg tgcccgtcct cgtaggaaac gacacccgtg cccgctgccg cgtggaccgc
     1741 cgttacgtag taggtaccgc cgaggaccgc gggctcgggg gagagcgccg cgatgttggt
     1801 ccggacatag ttctccgcga ccgagtcgcg ctcggctccg gagagcgccg ggggcggagc
     1861 cgcttcgggc tgcgggcgcg caggcggcgg cacgaaggag aacgcggcaa gcgcgatgac
     1921 ggcgaaggcc cctatccaga tgagtttatg catgaggggg atgataccag aaaccccggg
     1981 aatgtcgcat gaaccccgga ttcacgggcc attcatggcc gagggccgat actccgcaca
     2041 tatggcgtac atcctgtatt tcctcgcggg gctcatcttc gccaacggcg tcccgcactt
     2101 cgtgaagggg atcatgggcg aacggcacga cacgccgctc aaggggcctt ccggcgcgat
     2161 gctgaacgtg gtctggggca cggcgaacct cttcgtcgcc gggtggctct ggctctacgc
     2221 aagcatcatg ccgcaggcgc tcgggttcgc gctcgtcgcc ttcttcctcg gcatcctcgt
     2281 cgccggcatc acgctggcca agcgcttcga agcggaccgc cgggaggcgc agttctgagg
     2341 ctagaaccag gcgaagagcg cgaggatgcg ctcccagagc gccgagagga agcccttcgg
     2401 ggcttcctcc gctaccggct cggcggtcgc cacgggctcc tcggtcgcgg tcgggacctg
     2461 cggcgcgggc gcatcgtacg tcgtgcagta gcctgcgggc atggggccga tgcaggcctt
     2521 ctcggtgcag accgcggtgc cgtccgattg gcggctgcag tgattgcagc catcgaacca
     2581 cgcgctgcag tgcgccggcg gctggtagga aggggtcggg attaccggcc cggcctccgc
     2641 ggccgtgcac tccccttcat ggacgagggt ggcatggtca ttgcgcatca cgcaggcatt
     2701 gccgtaggtc tggtactgcg gatagcaggg cgcacgcacg cactggacct ccttcgcccc
     2761 acagacgggc tggtagatca tggtgcaggc cccgtccgga tgatgggcgg ccgcaggagc
     2821 cgcaagcagg agcagtgcag cgatgaggaa tgccttcatg cctatgcaga cgcggatggc
     2881 cgcgcctcct tacctagaag tacccgaagg cggtaccggc gaggtagatg ccggccacga
     2941 tcgcgagcgc gccgcccgcg tggtaccaca tcggcttatt gaaggaccgg atgaagtgct
     3001 ggacgaactt cgcgggcatc gccatataga cgaggctctc gatgaccatc atgtagccga
     3061 cgagcgagat gatgccggca agcgacactg atacctgcgg gtacgcgatg acgatcgcga
     3121 gcccggcgac gagctcgatc gccgcgatca cgaaccggag acccgtattc gaggcgaact
     3181 cccggatggc cgggatgatg gacttgcggc gaagcaggct catcacgccc atgataatga
     3241 agtaaaggcc gagcagcttg gcgaggaata tttccatacg cagagttaat gactatgcgc
     3301 ctacgatagc actggttagt gcttctggat gaggttatgc acttccctac tccgctgcgc
     3361 tgcggcgatg gaagtgcggg atgcggggcg caaggatgct caccaccgca gcgatcactg
     3421 caagcacgta gaagccgaag ccaatcgcca tcccgatcgc gcccgcgacc cagaggcttg
     3481 ccgccgtggt gaggttcttc acgctcgcgc cctccttgag gatgagcccg gcgccgagga
     3541 agccgatgcc tgagacgatc tgggctgcga tgcgcgacgt ggacatagga tcgacgatgg
     3601 ccgagatctg ggtgaacagc gccgcgcccg cgatcacgag cacatgggtg ctgatgcctg
     3661 catccttgcc gcgcgcctcg cgctctgccc cgatgaggaa gccgaggagg agcgcgaacg
     3721 ccatcgcgac caggaactgg agctctaccg gcccgatgat gccttcgatc atagctctag
     3781 tgtagcacta gggagtaacg ggctcgtagt agataacgat ctggtccttg tcccgcagga
     3841 tcgtgttctc gaaggtatcc acttccgccc cgttcaccgt catgcggatg cggtgggtat
     3901 cgtccgcgac atgctcgagg atctgcgtgg cactaaaggg cttctcccag ttagcgaaga
     3961 aatcggccag cgtgaaatcg cgcggctcgt tcgattcgat atggatgatg ccgtcgggcg
     4021 tatgggtatg gatcgcccgc atgcagcccg gcgtgatgcc gatatccgcg gggacgacga
     4081 tcggctcgcc gttcaccatg atggaaagcg tcggatggat atggaaggtg tcggattcat
     4141 gggtcatgca ggacatcgcg atctcgcggt tcgtccgctt agtggccgta ccctcgcggg
     4201 tcgaggcgga cgcgaaccat atgagagcga gcaatgcgct gatgccgatg atccagagcc
     4261 aggtgttctt cttcatgcga ggcagtatag caaacgcgct agcgcgacgc gtagaggtca
     4321 accagatacc cgtcggggtc ctcgacgacc gcataccgct ggccccagaa ggcgtcccag
     4381 ggttccttcg cgatcgcaaa gcccgcttcc ttcacggcag cgacggtggc gtccacctga
     4441 tcgggggtac cgcagttgat cgcgatcgag gcatggttgc cgggcttagg cgcatacccg
     4501 aggatgccct gcatcatggt ggcgtcgtcg atcataaacc gtacccctcc cgcttcggta
     4561 acggcatcaa catgctgctg tcccgtatat tccggaaagg agaacccgag caaggaatag
     4621 aattggatgg tcttcgggat attagtgctg gtgacttcaa cggcatcgaa tttcatgtga
     4681 gtggtattag cgggtaaacg gcacctcccc gatccgcagc cactgatctg ctggtcccac
     4741 ggtgcgatag agagcgaggc ggtcgcagcg gaaggattct ccgctcggat attcgcccgc
     4801 gctcctcatc gcttcgcgca ccccatcatc gatgttcccg tagtagaggg aaaggtgcgg
     4861 acggtagggc tccgccggcg tgccgaaggt ctgctgcgcc acttcatgcg cgctcatgag
     4921 ctccggatcc tccttgatgg tcagatagaa cgcgcggaag aagctgggct cgccaccaag
     4981 ctccccgagc atgaccgtga gtggcggaag ctgctgcgcg agcatttgtg cgtgcgaaag
     5041 gatgtcctcc tcatttccct cgagattcgc caggatggtg acgtgcgggg ggaatatcgg
     5101 gccgtcgtag gcggcggcaa gcgtcctgat cgtttcctgc aatcgctcat tgagcgcgcc
     5161 cgtcggctcg aggaagaggt ggtagcctgc ggtctcgttc gagcgcgggt ccatcagccg
     5221 cggtacgccg cctcgagcgc ggcgatatcg atcttctgca tcttgagcat cgcgctcatg
     5281 acccggtcct gctttgcggt atccgggtcc ttcagcatct cactaaggat tatcggtgcg
     5341 atctgccacg agacgccgaa tcggtccttc acccagccgc aggggccctg ctccccgccg
     5401 ccctccgtaa gccttgcggt gtactcgtcc acctccgcct gatccctgca gttcacgagg
     5461 agcgagaacg cctcgttgaa ggagaaggta tggccctcgt ttgcgctatc catcgcgaag
     5521 aactcctgcc ctgcgagcgt gaccgccccg tgcatgaccg tgcctgctat ctcaggctcc
     5581 atgcccgcgg gccaccgagc gatatccccc agcttgctct ccgggaatat ggacgcatag
     5641 agagttatcg cttcctcggc cttccccgct accttgccca cgaacaggaa gctcgtcgcg
     5701 atccggttct tgtacggata gtgcccgtca tcgagcacga gctgccacga cagtccatac
     5761 cgatcgctgc accagccgta ccgctcgctg aagggatacg catcgagggg catcaggatg
     5821 ttgccgcctt cggagagcgt agaccagagc cgatccactt cttccttggt cttgcaggcg
     5881 accgtgaagg atatcgaggg attgagcgtg aagtgcggtc cgccgttgag cgcgatgaag
     5941 ctctgcccgg caagctcgaa ggcgacggtc atgacggagc ccggctcctt gccggtgatc
     6001 tcctgacccg cgttcgggta atgggtcacc tgcgtaatgc gcgagtccga gaagagcgag
     6061 gtatagagct tggcggcttc ctcaccttcg gtatcgaacc agagattcgg gacgattttc
     6121 tgcatggtct tggcgttagg ttcaacgatt caacgagtag tatgccccta gcatatacca
     6181 ggcgcaggtg aagcaggggt gtacgctagg gccgtccgcc gatctgcggg ttgtgcttct
     6241 ccttgatctt ctcaatcttc tgccggagcg cttccggcat atccacgccc atggccttcg
     6301 caagcaggaa ggtaacaatg accacgtccg cgaactcgcc ctcgaggtct cgctccgcgc
     6361 gcatcttgtc ccggcgctgg tcgccggcgg ccgcaagcac ttcgtcgcag agctcgccgt
     6421 actcctcccc gagcttgatg gtccgggcga agatggcctc ggtcccctgg ccgcccttat
     6481 tgaccgactt gaacaatgcg tcctgcgcat cgatgaaggc ctggaattcc tcgaaggtca
     6541 tgcggtccgc catatcctag aggtgtatgt cgatgcggta gccgtgggcg ccgctgccgc
     6601 cctcaggatc gttcatccag accttcctcg ggatggactt gtagcactta cggaccgcgt
     6661 gccccgcgaa ctcctcgagg tccggcgggc tccccatgcg attggcgttc cggacccgct
     6721 gtatctccgt cggatcggtg agctcgatga cgtccgcttc catgtagacg cggtctcgct
     6781 cgctgccggg gtccgcgagg tcgtgcacca ccacgacgat gcgggggttc gtatggatgt
     6841 tgcgcgcgtg ctggctgtcc ttatcgcaga accagtaaat attgaaatcg gtgtcgatca
     6901 ccggcgcgat cgggctcgac caggactttc cgtccgaggt gacggtagcc acggtcgcat
     6961 agcgaatctt ggagagaatc tcacgagccc gcttatcgtg gcgctcgccc ataccggaat
     7021 catagcagct gagcttgggg gcgctagggc tcttacgagg cggccatctc aaggagcttc
     7081 tcgaggacga tgggcaaggt ggtgaacccg gccccttcgt tgaaatggcg ctggccatgc
     7141 tggatgaggg tctctgagcc aagccggtcc ctgaaatctt tggcgttctc ggccggtacc
     7201 cacggatcgt cgtccgagaa gagcgcgaag ctctcgggaa gatgcgatcg gaccttatcc
     7261 gtgtcaatcg gggtattcgc ccattctgca tcgatctcac gctcctcgtc ggtctcgagt
     7321 ccggtaaggg gccggaagaa tcccgcgacg aagatcgcgc cacccacctt ctgtcctgcg
     7381 ggaagcgcct caaggtaccg aaggatagcc tggcacccca tgctgtgccc taccaaatac
     7441 atatgctcac ccacagtacc tattgcttcg gcgagtgcgg gcacccaggt tgcgatccgc
     7501 ggagcatcgg tatctggcag ttgcggcgca acaaccacaa agccgcgcgc ttcgagttcg
     7561 cgctgaagcc atggaaacca tccggtgttc ggggtgccgt cccacccatg gaccaggatg
     7621 actttcctca taggctctcg atgacctgtt tgccttcggg tacttggtaa ttcgtatagc
     7681 gccaccaggt cggcgtgaat acgaagaaca catcatcggg atcgccgtac tgctctcgct
     7741 tttccgggaa tttcgagaaa taagcctccc agtgcagctc ggaatccgta agccgtgccg
     7801 ttccatcgag ctgcagcgtc cgcatctggg attcatcggt accgacgacg aatgatgcgc
     7861 gcgtcgatgc accaccctgc atgggttcga ctttccggta cgtccggtcg gtaaggaata
     7921 cgaaagtgag cggatcccgc tggtacgcga aatgtacggt ggagccgtgc ggggcgccat
     7981 tcggcatttc taccgagata acacccacac gctgttgatc tagaaagtcg tggacttcgt
     8041 caggcatatc atttttgcaa tacgctccac aggttaatgc attcatattc cttctcgaag
     8101 aaggttgtcc gcttaacgag ggtgcgttgc tcaatgaggg tgaatgctcc gtacagttca
     8161 tcgatctcct gctcatcgaa ggtcttctcg aattttccgg tcttcggatg caggaatgaa
     8221 cctttctccg gccccggcga atttagattt atatccttat ggtactcgtc ctcggaggaa
     8281 agcaggtagg cgaggagata cccgctcggt tttaggactc gatgcatctc cttcacggca
     8341 tgtgccctgc cttcgggcga ttcgatatcg gtagaggcaa agcagtcgat cccgaagtcg
     8401 aaatagtcgg attcataact ccaaggggtc gttgcatcct ggacctcgaa tgtaggattt
     8461 acaccggctg cgctggcccg tttacgagct tcggttattg ccgctggaga aaagtcgaac
     8521 ccgttcatct cgtacccctt ctgcgcgagc cagatgacat tgcggccttt gccgcacccc
     8581 atttctagac cacgaccctg attaacgccc tgtgattgga gaaagtcata aaaagcgagg
     8641 acgccgcttg aaacatcctg cgaatccatc tgtttgagca ccaacggatt cttgtgttcc
     8701 tcatcccata ttctttgctg atcctgatgc gtaaactctt tcatgggatc agtatatatg
     8761 ctgtgagcat cgagcggggt tggggcaagc ctaaacggta tttaaatatc taaggagcac
     8821 aagagcgctc cgttcctcag aaatggatcc gtccaggcac atgtgcctta ccgtatcgag
     8881 gtcgaagaac tcacgcacga tatcctctcc ctcctcaagg tgctgatcgc cttcctcgaa
     8941 gtcctccacg aggaagtaga aaaggtccca cacgacggtc gctccgcaca cggacttgtg
     9001 caggagcgag agcttctttg aggtgatgcc cgcctcttca cgcgcttcct tgatggccgc
     9061 acgcttcgcg gcttcctcga gatctgcatc cgatccgagg gcggcattat attcatccag
     9121 ggaatcgtag accttcccgc cgggcaaacg gaaatcgtat ccattcagtt cgtggcggaa
     9181 ctccttcgtg agcaggatgc gaccgtcctt agggataatg aggcgagtac cgggcgaacg
     9241 acgagcaaac tcgaatatcc tgggcttgcc gccttgttca acctctttct gcacgacttc
     9301 aatgattttt cctcgatgag tgactctttc catgcgtgaa tcgaatgcta cttcagcata
     9361 tactcccacc ccggcttcca agacttcgtc ttgcgccagt cgttcgcgag tgcttcgtcc
     9421 cagctcattc ccttgaggag tacgtcgagt gagagctggg gtgccttgtg gccaacgagc
     9481 gcaacacttt tgccgtcata gttctctttt aggaacttca agaagtcagc aacgcggttc
     9541 ttcacgtcct cgtagctctc tccatttgga aacggtctgt aatgcatctc actctcctga
     9601 agaggctcta ctgcctcgga cggatgaccg ttcatgtctc cgtagttaca ttcccgaagt
     9661 ctcgcatctg gaatgatttg ataccgaccg ccccacgcga gctctgcgga cttatgtgcc
     9721 cgttctaggt cagagcagaa gaccacgtca aagtgcttat ccttggtttg ttctccgagc
     9781 gcgatcgact gctggactcc tagctcggaa agcggcgcat ctgaccaccc tgaagagatc
     9841 ccattctcgt tatctatggt tgtcccgtgt acgaaatagg tgatgttgac cataggggaa
     9901 atgtagcaat gctgcgggac taggactcga acctagataa acgcctccag agggcgctgt
     9961 cctaccatta gacgatcccg caataccgaa gcggccgaag ccgcttggtg cgcacagaat
    10021 acgccaaacg cacggcgtat tcaactgtac cgcggcggtg gcatggcggt tgcgctcgct
    10081 agacccagag ggtcaggatg acgcccatga tcgcaagcga tgccagatag tagccggagg
    10141 tgatgaagaa gtacttccag ctcctgttct cccagagcac catcgccaag gtcggcggca
    10201 ccacgaaccc gagccaattc cagaaggcgc cggtgagtcc tgcgacg
//
LOCUS       DASPKG010000007         9934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_957634, whole genome shotgun sequence.
ACCESSION   DASPKG010000007 DASPKG010000000
VERSION     DASPKG010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957634"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="VEA36_00275"
     rRNA            complement(<1..133)
                     /locus_tag="VEA36_00275"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(482..1159)
                     /locus_tag="VEA36_00280"
     CDS             complement(482..1159)
                     /locus_tag="VEA36_00280"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28788.1"
                     /translation="MFLTTWADRLALSFQDLFYGIVQYLPNLLVAVIIFVIGWLVGAG
                     LGRLVAQVINSLRVDQALKHAGVERVLDRAGFSLNSGAFVGMLVKWFFIIVFLVASLD
                     VLGLTQVTEFLRGVVLTYIPQVIVAVLVLLVAAVIAEAVERVIAGSARAANIRTANLL
                     GTIARWAIWIFAVLVALDRLGVSPLVQTLFTGVVVALSLAFGLAFGLGGQAAAARYLD
                     RVREEIK"
     gene            complement(1287..1865)
                     /locus_tag="VEA36_00285"
     CDS             complement(1287..1865)
                     /locus_tag="VEA36_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28789.1"
                     /translation="MEPLARLFQNPVRLKLLRLFIFNDDTCFTAAAASFRTKSTKAAA
                     RREITVLLAADVIRKKTGRNAGYMANKKFVHYEPLKTFLRETTEVRDADMVAKVKRAG
                     TLRLLMLSGMFTGAIESKVDLLIVGDRLDEKRLATAIHGLEAELGRELRYAAFTTEEF
                     KYRRGVYDRLLRDTFDYPNRIILDRLGVSQTA"
     gene            complement(1974..2324)
                     /gene="rplL"
                     /locus_tag="VEA36_00290"
     CDS             complement(1974..2324)
                     /gene="rplL"
                     /locus_tag="VEA36_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HYF28790.1"
                     /translation="MVEAVEKMTVLELSELVKVFEKKFGVSAAAVAVAGPVAGGAAGE
                     EAKSSFTLELTDAGANKIQVIKAVKEVLGLGLKEAKDLVDGAPSVLKENVGKDDAEEV
                     KKKIEEAGGKVTFK"
     gene            complement(2464..2958)
                     /gene="rplJ"
                     /locus_tag="VEA36_00295"
     CDS             complement(2464..2958)
                     /gene="rplJ"
                     /locus_tag="VEA36_00295"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HYF28791.1"
                     /translation="MAITKAKKGEIVEKITDALKDAASVVFVHFKGLSVADTALMRKT
                     LREQGIGYYVAKKTLMRRALTGAGIKGELPELPGEIAMAWSSGDATLPAREIYEHGKK
                     HKDALTIMGGVFEGMFKDKAAMNAIATIPPVPVLRGMFVNVINSPIQGFVIALDQVRE
                     KKAA"
     gene            complement(3227..3799)
                     /locus_tag="VEA36_00300"
     CDS             complement(3227..3799)
                     /locus_tag="VEA36_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28792.1"
                     /translation="MRNLSAKQWYWIGGGATALLLAAAVYFQLASTTGTLGGDTATTQ
                     GLPPEPAFSLPTGATAVDDYAFTKPDGVYFRSLNDPEPLRIPDADPATFMRLIESRSY
                     ATDAVVRDCGGPATYTFYGDAERVYMHQYWRVPDKFRSSRIEVLVGADRPNFQVNEDH
                     TATDGARLYTINHQVSTTSTCWLMLSRSEL"
     gene            complement(3801..5771)
                     /locus_tag="VEA36_00305"
     CDS             complement(3801..5771)
                     /locus_tag="VEA36_00305"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HYF28793.1"
                     /translation="MKTTVAGTLAAIAVALLPFITSNQIFYGPVNAKFFFVLFVIDLL
                     ALIAAHQFYKRKEGIKAHGRWLLFALIFATVVQVLATLTGVFPERSLWSDIFWSSGVL
                     FLIHLAFLAFFLAELLNEQDWSLIRRAVAVSAGVFSALLIIGAQGFGATGPFLWLNLS
                     ENGLTFGNETYAGAYLLLAFGLGTIELLRTREWGRGRIVLLVSVILIALSPLMSNLPA
                     IASIAERPAALLGLARASSAAMFFILLFLAGYFLIRKLAPKKAGRLVLAWGAVLVLGA
                     ATGIGLLFTPGSAVQQAYIEESTAARIIVWDASMNAIAERPILGWGPENFNYAIERHF
                     DTRLFQDENLAEIWFERAHNVFIDTLVGAGALGLISFAILLLLSLVVIRRAHRKGLIG
                     DAEAALLAALLFAHVLQLQTGFDTIGSYTLLAIVLGYLLWLERGLLPAAGTPSRIVRK
                     GTVAVLVVLAAGSLIFCVLNEYTRQAALPDTFSARTLAAQAENREKSLERLSSFESLR
                     LSSASFIKGSLAVIAERSTEERRASILAVAHAYEERYQEYLAEQPDHYRAQMNYAYLL
                     LVMTTLGEDRVDDAKRAIERGYELSPGHPLTFVLDALAELYRGDLAESRRLLDEAAAI
                     NPDSEFTEEARTYFEEQYALFPNITVMKLTNL"
     gene            complement(5881..7845)
                     /locus_tag="VEA36_00310"
     CDS             complement(5881..7845)
                     /locus_tag="VEA36_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HYF28794.1"
                     /translation="MSIANNNIVARVAAVVAGLGLVASTFAFAPAASAQSSTEAQIQA
                     LMAQIAALQAQAGGSASVTFTRDLTLGSTGADVTALQNWLIKNGASIPAGATGYFGAQ
                     TQAALAKWQASVGITPAAGYFGPITRAKVNAAGGSTGGSTGGSTGGNTGGDLEGGAGS
                     VDSYELMGSLNSEEVGEDEEDVEVAGLEIEADEGSDLEFTAVRLVFDEGTAGSDFEDY
                     ASEVSVWFDGEEVARVDGDEFNDDNDWTATISLDGAVLEAGETGELTVAVSGQENLDS
                     DDLSDTWTVDFRQVRFMDADGATISEDPSTGTRTFSFVSFASAADVELKFAEDDDDIN
                     DARTVEVDATDDTNDVEVLSFTVEAEGDSDITIDSLVVSVTSTEATGNDPDDLISAIH
                     LVVDGEEVASETLSTSDTDNGEEKVVFDDLAWTVDAGETVEVMITADFYSIADTLDAG
                     DSVSFAITEAETDSSDFDAEDESGEDLSDAEVTGSASSGAFELRAAGIMVSFVSASEE
                     VTANDNAADNDVGTFRIVFNVTAFGSDVYLSRAAAATTATTIAATTDTAGHLYLVEDS
                     GTATTDDLTDIVTEMGDATEASSNNYLLQEDEETEITITVVQTNDTAEDDGIYRVLLK
                     AIAWATSDTTTWNVYDFDLEDFKTDPVNLN"
     gene            complement(8395..9162)
                     /locus_tag="VEA36_00315"
     CDS             complement(8395..9162)
                     /locus_tag="VEA36_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28795.1"
                     /translation="MTDKGHNREKDMRNSSTNDAHFDTFVLDKGIFSNVFDKDIRRVY
                     IYKKAERLAKAIHLVAPAFRNSLALREKLDLIAIGLVDAAVLPPALARTALSRELLAL
                     SSVLSIARAGGLLSPMNADLIARESHLLLQEVASYEEPRLALPDAPTLAALAKSAEGQ
                     MRQITERPVRQAPQAAHKGQSISDNKGHNSRKAAILEILNDKGPSYIKDISVIVRDVS
                     EKTIQRELQALVAEGRVVRSGERRWTTYSLKDSIATN"
     gene            9323..>9934
                     /locus_tag="VEA36_00320"
     CDS             9323..>9934
                     /locus_tag="VEA36_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28796.1"
                     /translation="MGRKKKEEVEAPKKLARIAGGIALIAFGLIFGLSAFGLAGLAGE
                     ALFAWGFGMIGLGSFLIPILMVLTGIAIMFERSFVKPLTALGAALIVVSVVVLFGMAS
                     ETLGGSIGRAAGITAIEFFGFAGALILLLSVALIGLAIVADLAALISGIGNLFGNTTQ
                     ALGSLKVPAVGDVAADVAMSIDTTDAPVETTPVEREPVVTMFNE"
BASE COUNT         1980 a         2900 c         3029 g         2025 t
ORIGIN      
        1 gccgggtttc cccattcgga cacctccgga tcgatggctg ttaatcgcct ccccgaagca
       61 tttcgctgat taccgcgtcc ttcatcgctt gcgcaagcct aggcatccac cgtgcaccct
      121 tcatgtctcc tgtgcggaaa cctgaacacc acttctaacg attctctttg tgatttgtgc
      181 aattctcgcc aggatggcat cgactcgatg ccacgcccct ggcgtgatta cctattttgc
      241 attttatgta gttgtcgagg tgctcgtgta ccggcaagaa aaaatcccct tccggggcgt
      301 cagttcctaa cccactaagg ttaggggatg gccgctccgt tagatgagta atttcttatt
      361 cgtaaacacg taggcggagt atacgcgcct cggttttagc tgtcaaacgg ggcctttcca
      421 ccggttttcc ccatacgcaa aaggcccctt ccggggcctc ctgccttccc tgctgaggga
      481 actacttgat ctcctcgcgc acgcgatcga ggtagcgggc tgccgctgcc tggccgccga
      541 gcccgaaggc aaggccgaag gcgagtgaaa gcgcgaccac gacgcccgtg aagagggtct
      601 ggaccaaggg ggagaccccg aggcggtcga gcgccacgag gaccgcgaag atccagatcg
      661 cccagcgagc gatggtacca aggaggttcg cggtgcggat gttggctgcg cgcgcagaac
      721 cggcgatgac gcgctccacc gcctctgcga tgaccgccgc gacgaggagc acgagcacgg
      781 cgacgatcac ctgcgggatg tacgtgagca cgacgccacg gaggaactcc gtcacctgcg
      841 tgagcccaag cacatcgagc gaagcgacga ggaagacgat gatgaagaac cacttcacca
      901 gcatcccgac gaacgcgccc gaattaagcg agaacccggc acggtcgagc acccgctcga
      961 cgccggcgtg cttgagcgcc tggtcaacgc ggagcgagtt gatcacctgc gccaccaggc
     1021 gaccgaggcc tgcgccgacg agccaaccga tgacgaagat gatgactgcg accaataaat
     1081 tggggaggta ctgcacgatg ccgtagaaga gatcctggaa cgacagcgcc agcctatccg
     1141 cccatgtggt gaggaacata tgcgtgtgct tctctggttt aggtaattaa tgctggtctt
     1201 ccggggccac tggccctgga caccctgcct attcataagt ctagcaagct gaagcggggg
     1261 tgtagcccct ggtgtggata ccgtatttaa gccgtctgcg agacccccag acggtccaaa
     1321 atgatgcgat tcgggtagtc gaaggtatcc cggagcagcc gatcgtacac tccgcggcgg
     1381 tatttgaact cctcggtggt gaaggcagcg taccggagtt ccctgccgag ctcggcctcg
     1441 agcccgtgga tggcagtcgc gagccgcttc tcgtcgagcc ggtcgccgac gatgaggaga
     1501 tcgaccttcg actcgatggc gcccgtgaac atgcccgaga gcatgaggag gcggagcgtc
     1561 ccggcccgct tcaccttcgc caccatatcc gcgtcccgca cctcggtcgt ctcccggagg
     1621 aaggtcttga gcggctcgta gtggacgaac ttcttgttcg ccatataccc cgcgttccgg
     1681 ccggtcttct tgcggatgac gtccgcggcc aagaggacgg tgatctcgcg ccgcgccgcc
     1741 gccttcgtag acttggtgcg gaaggaggca gccgcggccg tgaagcaggt atcatcgttg
     1801 aagatgaaga gccgcaggag cttcaggcgg acggggtttt ggaagaggcg ggccaagggt
     1861 tccatggact ggttgaggca ttgtacccag ccattcatga aggctgcaag cggtgctact
     1921 gggacacgaa aaatgcccgc tgtcgcgggc atcttccgtc gtgggggttg tcgttacttg
     1981 aaggtaacct tgccgcccgc ctcttcgatc ttcttcttca cctcctcggc atcgtccttg
     2041 ccgacgttct ccttgagaac ggacggcgcg ccgtcgacga ggtccttcgc ctccttgagc
     2101 ccgaggccga gcacctcctt cacggccttg atcacctgga tcttgttcgc acccgcgtcg
     2161 gtgagctcga gagtgaacga ggattttgcc tcctcccctg ctgcaccgcc ggctacgggc
     2221 cctgcgaccg cgaccgccgc cgcggacacg ccgaacttct tctcgaagac cttcacgagc
     2281 tccgagagct cgagcacggt catcttctcg accgcctcga cgatcgcctt gaactgcgcc
     2341 ggaacctcga cttccgcctc ggcggccggt gcctcggcag ccggagctgc ctcttctgcg
     2401 gggagggcgg tctcttcgac cgcggtctcc tcctgcttta cgtcatctgc catatattct
     2461 tggttacgct gctttcttct cacgcacctg atcgagtgcg atcacaaaac cctggatcgg
     2521 tgagttgatc acgttcacga acatgccgcg gagcaccggt acgggcggga tggtcgcgat
     2581 ggcgttcatc gccgccttat ccttgaacat gccttcgaac acgccgccca tgatggtgag
     2641 cgcgtccttg tgcttcttgc cgtgctcgta gatctcgcgg gccgggagcg tggcgtcgcc
     2701 tgagctccag gccatcgcga tctcgcccgg aagctccggg agctctccct tgatgcctgc
     2761 gccagtcagg gcgcggcgca tgagggtctt cttggccacg tagtagccga tgccctgctc
     2821 gcgcagcgtc ttgcgcatca gggcggtgtc cgcgaccgag agccctttga agtggacgaa
     2881 cacgaccgag gccgcgtctt tgagcgcgtc cgtgatcttc tcaacgatct ctcctttctt
     2941 tgccttggta attgccatag ctctgttggt tggttgtacg aaacggcgaa agtcccttgc
     3001 gggaccttcg aggagacaat cgaccgggag ccgtaaatcg gcttccgtcg ttcctcggca
     3061 gggcttatgg atgcctgctg tcttcggtcc gtacgggggc actataccct tccccgggcc
     3121 ggcaggcaac taggagacca ggcggatgat cgcgagggcg gcgacaagga tgacaatgaa
     3181 tcccgccacg aacttcagga agtggatgaa gtactgcatg cggggattat agctcgctcc
     3241 gcgagagcat gagccagcag gtcgaagtgg tcgagacctg atgattgatg gtatagaggc
     3301 gcgcgccatc cgtcgcggta tggtcctcgt tcacctggaa gttcgggcgg tccgcaccga
     3361 ccaagacctc gatgcggctc gaacggaact tgtccggcac ccgccagtac tggtgcatgt
     3421 acacacgctc ggcatcgccg tagaaggtat aggtcgcggg cccgccgcag tcgcggacga
     3481 ctgcgtcggt cgcgtaggac ctcgactcga tcagacgcat gaaggtcgca gggtccgcat
     3541 ccgggatgcg cagcggctcg gggtcgttca gggatcggaa gtagacaccg tcgggcttcg
     3601 tgaaggcgta gtcatcgacc gcggtcgcgc ccgtgggaag tgagaaggcg ggctcgggcg
     3661 gcaggccttg ggttgtggcg gtgtcgccac cgagcgtgcc ggtcgtcgag gcgagttgga
     3721 aatagactgc ggccgcaagg agaagcgccg tcgctccccc gccaatccag taccactgct
     3781 tcgctgaaag atttcgcata ttagaggttc gtgagcttca taacggtaat gttcgggaag
     3841 agcgcgtact gttcctcgaa ataggtccgc gcttcctccg tgaactcgct gtccggattg
     3901 atcgcagctg cctcgtcgag caatcggcgg gattcggcaa gatcgcctcg gtagagctca
     3961 gcgagcgcat cgaggacgaa ggtgagcgga tgcccgggcg aaagctcgta tccgcgctcg
     4021 atcgcgcgct tggcgtcatc gacgcggtcc tcgcccaagg tcgtcatgac gagcaagagg
     4081 tacgcgtagt tcatctgcgc gcggtaatgg tcgggttgct ccgcaaggta ctcctggtac
     4141 cgctcctcgt acgcatgcgc gaccgcgagg atgctcgcgc ggcgctcctc ggtcgagcgc
     4201 tccgcgatga ccgcaagcga tcccttgatg aacgaagcgg acgagagccg cagggattcg
     4261 aagctcgaga ggcgctcgag cgacttctcc cggttctcgg cctgcgcggc gagcgtacgc
     4321 gccgagaacg tatccggcaa cgctgcctgg cgcgtatatt cgttgaggac gcagaagatg
     4381 aggctgccgg ccgcaagcac gacaagcact gcgacagttc ctttgcgaac gatacgtgag
     4441 ggtgtgcccg ctgccgggag caggcctcgc tcgagccaga ggaggtaccc gaggacgatt
     4501 gcaagcaaag tgtagctgcc gatcgtatcg aagccggtct ggagctggag cacatgcgcg
     4561 aacaggagcg cggcgaggag cgcagcttcg gcgtccccga tgagcccttt tctgtgcgcg
     4621 cgacggatga cgacgagcga gagcaggaga agaatcgcga acgagatcag cccgagcgca
     4681 cctgcgccca cgagcgtgtc gatgaacacg ttgtgggcgc gctcgaacca gatctcggcg
     4741 aggttctcgt cctggaagag ccgggtatcg aaatggcgct cgatcgcgta attgaaattc
     4801 tccgggcccc agccgaggat gggccgctcg gcgatcgcgt tcatgctcgc gtcccatacg
     4861 atgatgcggg ccgcggtcga ctcctcgatg taggcctgct ggaccgcgct ccctggcgta
     4921 aagagaagtc cgatgcctgt ggctgcgcca agcacgagca ccgctcccca cgcaagcacg
     4981 aggcgaccgg ccttcttcgg tgcgagcttg cggatgagga agtatcccgc aaggaagaga
     5041 agaataaaga acatggcggc gcttgaggcg cgtgcaaggc caagcagtgc agcgggtcgc
     5101 tcggcgatcg aagcgatcgc aggcagattc gacatgaggg gcgagagcgc tatcaggatc
     5161 accgatacca gtagcacaat cctccccctc ccccactcac gggtccgcag gagctcgatc
     5221 gtccctaagc cgaaggcgag gaggaggtag gcgccggcgt aggtctcgtt cccgaaggtg
     5281 aggccgttct cggaaaggtt caaccagagg aaggggccgg tcgcgccgaa gccctgcgcc
     5341 ccgatgatca gcagcgccga gaagacgcct gcggagacgg cgacagccct gcggatgagg
     5401 gaccaatcct gctcgttcag gagctccgcg aggaagaacg cgaggaatgc gaggtggatg
     5461 aggaaaagga cgccgcttga ccagaagatg tcggaccaga gcgagcgttc cgggaagacg
     5521 ccggtcaggg tcgcgagcac ctgcacaacc gtcgcgaaga taagcgcgaa gaggagccag
     5581 cggccgtgcg ccttgattcc ttcctttcgc ttatagaatt ggtgcgcggc gatgagcgcg
     5641 aggagatcga tgacgaaaag cacgaagaag aacttggcgt tcacgggccc gtagaagatc
     5701 tggttggacg taatgaacgg gagcagggct accgcgatcg ctgcgagcgt ccctgccacg
     5761 gtggttttca tgggggaagc ttagcaatgt agaacgcaaa aaaccacccc gtagggtggc
     5821 tttttgcttg cctagctaag cagtgccgta gcgcttctta gttcagccaa ctaaccgtga
     5881 ttagttgagg ttgaccgggt cagtcttgaa gtcttcgaga tcgaagtcgt agacgttcca
     5941 ggtcgtggtg tcgctggttg cccatgcgat cgccttgaga agcacgcggt agataccatc
     6001 gtcttctgca gtgtcgttgg tctggaccac ggtgatggtg atctcagtct cctcgtcctc
     6061 ctggaggagg tagttgttcg aggaagcctc ggttgcatca cccatctcag tcacgatgtc
     6121 cgtaaggtca tcggtggttg cggtgccaga atcctccacg aggtagaggt gacctgcggt
     6181 gtcggtcgtt gctgcgatcg tggtagcggt cgtagcagcg gctgcacgcg agaggtacac
     6241 atccgaaccg aacgcggtga cattgaagac gatacggaag gtaccgacgt cgttgtcagc
     6301 tgcgttgtcg ttggcagtca cttcctcgga agccgagacg aaggacacca tgatgcccgc
     6361 tgcgcggagc tcaaaggcgc cggacgaagc cgagccagtg acctctgcgt cagacagatc
     6421 ctcacccgac tcatcctcag catcgaagtc agaggaatcg gtttctgcct ccgtgatagc
     6481 gaaggagacc gagtcgcctg catcgagcgt gtcagcgatc gagtagaagt ctgcggtgat
     6541 catgacctca acggtctcac cagcgtcaac ggtccaagca aggtcatcga agacaacctt
     6601 ctcttcgccg ttgtcggtgt cagaggtgct gagggtctca gatgccactt cctcaccgtc
     6661 caccacgagg tggatagcgg agatgaggtc atcgggatcg ttacccgtag cctcggtgga
     6721 ggtcaccgat acgacgagcg aatcgatggt gatgtcggag tcaccttcag cctcaacggt
     6781 gaaggagaga acctcaacat cgttcgtgtc atcggttgcg tccacctcga cggtgcgagc
     6841 atcattgatg tcgtcatcat cttcagcgaa cttgagctcg acatcagctg ccgaagcgaa
     6901 ggacacgaag gagaacgtac gggtacccgt cgaagggtcc tccgagatcg ttgcaccatc
     6961 agcgtccatg aagcggacct ggcggaagtc aacggtccac gtgtcgctca ggtcatcgga
     7021 atcgagattc tcctgaccag aaacagcgac cgtaagctcg ccggtctcac ctgcctcgag
     7081 gactgcacca tcgagagaga tggttgcggt ccagtcgttg tcgtcattga actcatcacc
     7141 gtcgacgcga gcgacttcct cgccatcgaa ccaaaccgag acttcagaag cgtagtcctc
     7201 gaagtccgaa cctgcggtgc cctcatcgaa gacgaggcgc actgcggtga actcaaggtc
     7261 agagccctca tcagcctcga tctcgagacc tgcaacctcg acatcctcct cgtcctcacc
     7321 gacctcctca gagttgaggg agcccatgag ttcgtaggaa tcaaccgaac ctgcaccgcc
     7381 ttcgagatcc ccgccggtgt tgccgccggt tgagccgccg gtgctgccac cagtcgagcc
     7441 acctgctgcg ttcaccttag cgcgggtgat cggaccgaag taaccagctg cgggggtgat
     7501 acccaccgaa gcctgccact tggcgagagc cgcctgggtc tgggcaccga agtaaccagt
     7561 tgcgccagcc gggatcgaag cgccgttctt gatgagccag ttctgaagcg cggtcacatc
     7621 agctccagtg gaaccgagag tgaggtcgcg ggtgaaggtg accgaagccg aacctcctgc
     7681 ctgcgcctga agcgctgcaa tctgggccat gagggcctgg atctgagctt cggtcgacga
     7741 ctgcgcgctc gctgcgggag cgaaagcgaa ggtcgatgca acgaggccaa gacctgccac
     7801 cactgctgct acacgagcaa caatgttgtt gtttgcaata gacataacaa ttgcgttttt
     7861 aatttatgtt tcctgagata cgtatgcaac gcgattagta atagtgcatc gcatacgcat
     7921 ccgtatcttt ctttgtaatt gtgctttgcg gacctgtcgg cccgctcggc gataaacctg
     7981 ttttccttac tagaccaaca gagcgctcta tcgacgcgcg ccccatcggc tagctggcaa
     8041 agtgagtttt cgttctgcgt tcatccgtaa ctgagtaccg cattcgtgct gcacccgccc
     8101 accggtaggc agacggctaa gctcatgggt tcttacgaac cataagcccc gtaaatccct
     8161 attcctttat caaggattca gtcgttcagg agtacgcacc agctcgtgtc tgtatgggag
     8221 acagtatacc gagcaattac gcgggttcct gatatttcaa tgtggataac cgccgggttt
     8281 ggaaacgtaa aaggggcctc ccgcctttcg gcagtggcct ttcctatttc ctgtccccca
     8341 tagttcccac atccttaatc ctaggccaga tgagcttaaa agtcaatgta gagcttagtt
     8401 ggtggctatg ctgtccttaa gggagtaggt ggtccatcgg cgttcaccag accgaaccac
     8461 ccttccctca gcaaccaagg cctggagttc ccgctggatc gtcttttcgc tgacatcccg
     8521 aacgataacc gatatatcct ttatatagga cggccccttg tcattcagga tctcgagtat
     8581 ggctgctttt cgtgagttat gtcctttatt gtccgatatc gactgtcctt tatgagcagc
     8641 ctgcggcgct tgccggaccg gacgctctgt aatctgccgc atctggcctt ccgcagactt
     8701 ggcgagcgcc gctaacgtgg gcgcatccgg tagagcgaga cgcggctctt cgtaggaggc
     8761 aacctcctga agcagaagat gagattctct cgcaataaga tcagcgttca ttggcgagag
     8821 caggccccct gcccgggcaa tggaaagcac tgaggagagt gcgaggagct cccgggagag
     8881 ggccgttcgc gcaagggcgg gtggaagcac tgccgcatcc acaagcccaa tcgctattag
     8941 gtcgagcttc tcacggagcg ccagggaatt tctgaaggca ggagcaacga ggtggatcgc
     9001 tttggcgaga cgttctgcct tcttatagat gtataccctc ctgatgtcct tatcaaatac
     9061 atttgagaat atacctttat caaggacaaa ggtatcgaaa tgagcatcgt tcgtcgatga
     9121 attcctcatg tccttttccc tgttgtgtcc tttatcggtc attgattctg tcctttaaat
     9181 caaggacaag tatacgcttc gggcgatgtc cgtcaaaggc ggcgctggga gcgctggcgt
     9241 ccttacaacg ggttccccga ccgattagag gtgcaggtag cttatgcgtc ttatccacag
     9301 cacgagtgtt agaatgctga aaatgggacg caaaaagaag gaagaggtgg aggctcctaa
     9361 aaagctcgcg cgtatcgcgg gcggcattgc gctcatagct tttggcctca tcttcgggct
     9421 ctcagcattc ggtcttgcgg gccttgccgg cgaggcgctc ttcgcttggg gcttcggaat
     9481 gatcggcctt ggatcgtttc ttattccgat actcatggtg cttaccggca ttgcgattat
     9541 gttcgagcgt tccttcgtga agcctcttac cgcgctcggg gctgccctca tcgtcgtgtc
     9601 cgtcgtcgtc ctctttggta tggcgtcaga aacccttggt ggttcgatcg gccgcgcggc
     9661 cggcataacg gcgatcgagt tcttcggatt cgccggagcg ctcatccttc tcctttcggt
     9721 ggcactcatc ggtctcgcaa tcgttgccga ccttgcggcc ctgatctcag gcatcgggaa
     9781 cctcttcggc aatacgacgc aggcacttgg ttcactcaaa gtgccagccg taggagatgt
     9841 cgcagctgat gtagcaatgt caatcgatac aactgatgcg ccggttgaga cgacgccggt
     9901 cgaacgcgag ccagtcgtaa ccatgttcaa tgag
//
LOCUS       DASPKG010000008        16110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_416243, whole genome shotgun sequence.
ACCESSION   DASPKG010000008 DASPKG010000000
VERSION     DASPKG010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16110)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16110
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416243"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VEA36_00325"
     CDS             <1..583
                     /locus_tag="VEA36_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYF28797.1"
                     /translation="MIVPIIRPKSFVGPERLGVFALFYDWAADGKGFPMIGSGNNRYQ
                     LLDVEDLCEAIYLASILPPEQANDTFNIGAKEFTTMKEDYQAVLDRAGFGKKIRGFPA
                     APMIWTLRVLEFFKLSPLYKWVYETASKDSFVSIDKAEQKLGWKPKYSNKDALVRNYE
                     WYLGKRDEFAGASGVTHRVPWKQGVLSIAKRFF"
     gene            586..2025
                     /locus_tag="VEA36_00330"
     CDS             586..2025
                     /locus_tag="VEA36_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28798.1"
                     /translation="MDRRHIGIAIAAGAFILLTAWQATHLHRYLAPDSFTYFAMAKEF
                     HESELLYSYSGVDHTTGIHPGYYFLLLPLYPGVGAALPAASFILNALFILGGLFLLYR
                     ALGFMPTAVIFLLTLTSQAAGALNNGMESSLLFFALSLATYLYLAKVEPGDTPSLLLL
                     GAALALALFARLDAIFFVAALYLVFAYRMLMQDRSINGLIGHVRTFMALSLPVFAALG
                     IIFAINLTYDGTLLPLSGQLKSSLPHVLPNWAASLPLLKMFIAGIAAMGAYLVYRMLK
                     RRPLQELVPALFIGSIALFTYNLFYVSGIGAWYGALPFFGAALALGLLVHDLWTDVPG
                     RYRRISTALVLVAFAVAVPLGHANRIVTDWITPNQAIAEELDRQAADGEAAAEFKDGV
                     VAFYSRVPVYSLTGLANNQAYVDAVRKGTLEAYLAERGIAYIAHGIGGSGLQVPEAVE
                     SIECTRPFYEKDGALLSRVADCAVVPTSR"
     gene            complement(2012..3055)
                     /locus_tag="VEA36_00335"
     CDS             complement(2012..3055)
                     /locus_tag="VEA36_00335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYF28799.1"
                     /translation="MKLLYATSMTYPSALANRVQTLETARALAARLGSDFTLGVSAGN
                     PNMPVKVMGSAPSPLLALRYLSLIRREGYTHVLLREERLLFFLALYAKTLGIPARFYL
                     EAHSIYRDLLLAPALRSATGIIAITNGLREDLIKEFGIPSAKVAVVPDAVDAAAYERV
                     ESKEAARRRLGLPQDAPLVMYAGRLDGWKGLETLLAAELPEHTELVIIGGKPPEVETL
                     QRRHPKVRFLGQRPYEELPQNLAAADVVVLPNTAREAISARHTSPLKLFAYLASGTPL
                     VASDLPSIREVVDEASVTLVAPDDPAALAAGIASVLADPEAAAVRAARARELVQDYTW
                     EKRAERLLAFIGR"
     gene            complement(3052..4065)
                     /locus_tag="VEA36_00340"
     CDS             complement(3052..4065)
                     /locus_tag="VEA36_00340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYF28800.1"
                     /translation="MQLLITTQILDRSDPVLGFFHAWVAALAKECEQVTVIALSVGEH
                     SLPGNVAVRSLGKENGRRGKLTYAATFQDHLRELDGTYDAVFVHMNPEYVLLGFPFWR
                     GKKVALWYNHDHGGWKLKLASYFVDALLHTSPYAASAGTKKSQQMPAGIDTQVFRPQG
                     VDVRLKSIYFQGRVSRSKRVHVLLEALRSVPGATVTLVGPAEPSYEAMLRSEFKDLIY
                     GGRVTFYGPVPNAETPALYAAHAVSVNLTAAGNYDKSVLESIACGTPALVSSPAFADL
                     VPEPCYFKENDPASLAETLRRILALSSEERRMLTDAGSARIHEEHSLGALTRAILEVY
                     RRP"
     gene            complement(4069..4923)
                     /locus_tag="VEA36_00345"
     CDS             complement(4069..4923)
                     /locus_tag="VEA36_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28801.1"
                     /translation="MRIGFIGQGYIGKNYADDFEKRGYDVVRYALEEPYVQNKELIKD
                     CDVVFIGVPTPTVPDGAGGIRFDDSIVRGAVGLVGVGKIAVIKSTVLPGRTVDIQKQY
                     PDRIVIYSPEFLREVRAAEDVANPFANIMGVAVEDDLHREAAETVIAMLPPAPYASII
                     TSTEAELVKYAHNGSGYTQIVFFNFMYDLAQKLGYDWERIGEALRADPLISHTYSNPV
                     HKAGRGAGGHCFVKDFAALRHMAEKELGSDHPSVRTLQAAEAANRAILAQSGKDKEIV
                     DGVYGPSA"
     gene            complement(4925..5557)
                     /locus_tag="VEA36_00350"
     CDS             complement(4925..5557)
                     /locus_tag="VEA36_00350"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYF28802.1"
                     /translation="MSQAETIYEAHHKARRAPGFSILKDDRGAFIREAVGTGKRILDI
                     GCRDGALTRTFMEGNTVTGVDVDREALARAGELGIEAIQMDLLGDWEELGDRQFDAVV
                     AGEILEHLYFPEQVVCKIRSRLKEDGVLVGSVPNAFSLKNRLRYLRGTKKHTPLSDPT
                     HINQFSAPELEALLARHFREVTVTGLGRYTRLAKLSPNFFAFDLAFVARP"
     gene            complement(5604..6734)
                     /locus_tag="VEA36_00355"
     CDS             complement(5604..6734)
                     /locus_tag="VEA36_00355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYF28803.1"
                     /translation="MKALFIGSDPTIFTPESPARTRLKRYAAEIGTLHVLSRGPHATD
                     LEDGPLHLHAVDGGKLAALHAMQGKARRLIKEQGIEIVSAQDPFEHGWVAYQAKKGTS
                     AKLHLQVHTDFCSPWFVKGTMLRAAHVQMPVLNTIRRSIADRIIPRADGIRAVSERVK
                     ASLIGRYGDRPISVIPIAGPESLPPRVALPPHPFSFAFVTVGRLEPEKRIEDAIDALA
                     RVARTYPMVGLIVIGDGRSRRRLMKRARMLGLGERVMFLGWRSDALGLMQSAQAYIQT
                     SAYEGYSLTLLEAARARVPIITTDVGIVGEVFTGYEDVLVAPVADPAALAVHIAGLVE
                     DVQARQSLVRNAERKAAAHLASIGDQPARIAEDLARTLAAPR"
     gene            complement(6740..7750)
                     /locus_tag="VEA36_00360"
     CDS             complement(6740..7750)
                     /locus_tag="VEA36_00360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYF28804.1"
                     /translation="MKLLIASPIGLQDIGGPATVARELSRVYEEHGDDVRVITPSWFE
                     KRLPIGVRQLVLFIRAIPTVRWADAVLLLDPVSTGLAVAPLARLLKKRSVLRVGGDFL
                     WEMHANRVREPIRLSEFYAMPRVLTLREHLIHEATRFTLAFVDHIAFTTAWQERIWME
                     PYNLSGRSTSVVESGVPEPMEAATTDTSSKVFLAASRGPYLKNGDLLAELWPNIQMRH
                     PEAELDTTPRPPEAYRQALAASYALILPSISDVCPNAVLEAIAYGKPFITTKDTGLYE
                     LYPESGMFVDTRDPNEIVAAIEKLLDPAGYRDAVARMRSSRRRRTWEDAAAEFRTLLA
                     VP"
     gene            complement(7754..8956)
                     /locus_tag="VEA36_00365"
     CDS             complement(7754..8956)
                     /locus_tag="VEA36_00365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYF28805.1"
                     /translation="MKKVLIFDMQYYPFVGGAEVAIKEITDRIPAGEVEFHLVTCRFD
                     STIPKTERLGNVTVHRIGFGVPHPGIGDLKKFPLHLNKHVYQLLAAWEALWLHRRYRY
                     DGTWAMMAHSAGIPAGIFKALNPKVRYLLTLQEGDPPEYIEGLMRPVMPLFRQGFTKA
                     DELQAISTFLLRWGKRMGFRGEGIVIPNAVDTAHFAKPIPDTDVRAMQEKLGKKESDV
                     FLVTTSRLVHKNAVDDVIRALPLLPEHVSFIVFGIGPDEEMLKGLATELGVAHRVQFR
                     GQIGHAEMPVALRACDIFIRPSRSEGMGNSFVEAMAAGLPVIATQEGGIADFLFDEKR
                     NPDKEPTGWAVSKDSPEEIAGAVRDIIANPEKVQRVTATAKAMVIEHYDWNTVAASMQ
                     SLFRRLLR"
     gene            complement(9042..9812)
                     /locus_tag="VEA36_00370"
     CDS             complement(9042..9812)
                     /locus_tag="VEA36_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYF28806.1"
                     /translation="MSATLRLVPALAAATKIHAEDPSKPLWQPGWKLRVNGEEMPAET
                     DWEVTSRFGAVRPAVVPHADGTPNFDRPLYSEAPNVNIVAYGVEDDGTVRIAIIRQPR
                     PHANDPLQPGVDGHAPVVFGQIAMGFLEKIIGETAEEAAQRETDEETGGCHVLSMFQP
                     AYPYHNPNPTFVDTWSDLWFVQVNLKRINELKFDRNEPIFSAEYVTVEELFSRIKSGV
                     DADGAVYRMCTANSILMIFFATFPQYLAGVVGKELAAV"
     gene            complement(9884..11356)
                     /locus_tag="VEA36_00375"
     CDS             complement(9884..11356)
                     /locus_tag="VEA36_00375"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYF28807.1"
                     /translation="MSKIILVTGGAGFIGSHLCERLAKTGYRVISLDNYFTGSRENHV
                     PGVEYREGHTKDIEQLVPEAPDLVYHLGEYSRVEKSFEDIEALWDLNKTGTFAVLEFV
                     RRRGAKLIYAGSSTKFADGGIGREQSPYAWAKATNTELVRNYGAWYGIDYAITYFYNV
                     YGRRERAGAFGTVIEIFKRSYLAGRPIGLVAPGTQERNFTHVDDIVDGLLLVGEKGHG
                     DDYGLGDERAYSILEVARMFSEDIVMLPERSGNRMASLLETEKARALGWKPQRSLAAY
                     IEEIKRTSSPSAPKGNRILVFTTTFHPIMGDAERALALLMEKMPDVEFDIVTAMHTPE
                     ASGTATFGSNARVYHVGWGTRFDKYLLPFWGAAIGHRLAKEHDYLFSWSVMASYGTLA
                     ALAVRRRATLPLLVTLADQRLSWYERLFLRLVMGQADQVYASLPEQSRTIASLARRMR
                     DRRSLGEGDAFANQIRFAYGSFLAKLQQEKRMRDSSSRGA"
     gene            complement(11524..13257)
                     /locus_tag="VEA36_00380"
     CDS             complement(11524..13257)
                     /locus_tag="VEA36_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HYF28808.1"
                     /translation="MENPERVTYFAATDSRGKKTPFGIKAKDRARHMYVIGKTGMGKS
                     TLLENMAIQDIRNGEGMAFIDPHGSAIETLLDFIPEHRIKDVVHFAPFDLDMPIAFNV
                     MEDVGYDKRHLVTSGLMATFKKIWQDAWSARMEYILTNTLLALLEYPGATLLGVNRMY
                     TDKVYRKKVVENIKDPVVKDFWVKEFMTYTDRYTQEATPAIQNKIGQFTSNPLIRNII
                     GQPKSSFDIRELMDQRKILLINLSKGQVGDVNMRLLGSMLTTRLFLGAMSRAELPQHE
                     LAKAPNFYFYVDEFQNFANETFAEILSEARKYKLNLIIAHQYVEQMEEEVAAAVFGNV
                     GTTIAFRVGPFDAEKLEVVFAPEFTPEDLVNLGFAQIYLSLMIDGIGSRPFSAVTIPP
                     IERPPRSWRQEVIEASRTQFGNPRAGIEKSIYDELSLNPDEAAPEPSPKRYLPPAGNQ
                     RPRPAEPRRENRAPAPRQEARGAPAASARTADDLKAILKSTIAGVKTEKEQKTKQSEE
                     SLKSALADVVKKEQPAPKPHTQQAQPKQQQQQQPRQENRRPQQPEHTRNTQSVFEVPE
                     DVLRDIFKDKF"
     gene            complement(13366..13554)
                     /locus_tag="VEA36_00385"
     CDS             complement(13366..13554)
                     /locus_tag="VEA36_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28809.1"
                     /translation="MGFYPPTPAAEARAHIWRYKNWIRLAEGYQTVCPIEWLLKGLWL
                     FWLRSQLVDAEEREERYR"
     gene            complement(13612..14682)
                     /locus_tag="VEA36_00390"
     CDS             complement(13612..14682)
                     /locus_tag="VEA36_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28810.1"
                     /translation="MKHLHRLFRNPVAILVGFGLVFIAVTVHGIPYAIAMHQNYAKTA
                     TTSVVAKLIPPAKPTLDTKLYDTQLERMANYASTTATSSRLWPVKTSYPKAGAILPMK
                     RVVAYYGNFYSTRMGILGEFDEATVLARLKGEVAKWEAADPETKVVPAIDYIATTAQL
                     HPQADKTYRLRMPDEHIQRAITMARKVNGITVLEIQAGLAPLQSEITAFEKYLVQPDV
                     HLAIDPEFNMKGGEAPGTVIGTVSASDINRASAYLAELVRKHDLPPKILVVHRFTQRM
                     VTDYQLIETRPEVQIVMVMDGWGPPAKKINTYKSFIYPEPVEFTGFKIFYKNDMKPPS
                     ERLLTPADLLKLTPRPMYIQYQ"
     gene            complement(14654..14848)
                     /locus_tag="VEA36_00395"
     CDS             complement(14654..14848)
                     /locus_tag="VEA36_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28811.1"
                     /translation="MSRESLLMVLGILIVVSPFTGLPLSLLTWFYVAAGLLIAAIGFT
                     LRLRRRRRAITHEAPPSALS"
     gene            complement(14873..15556)
                     /gene="rsmI"
                     /locus_tag="VEA36_00400"
     CDS             complement(14873..15556)
                     /gene="rsmI"
                     /locus_tag="VEA36_00400"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HYF28812.1"
                     /translation="MQGTLSIVATPIGNLADITFRAVETLKAADAIACEDTRVSAKLL
                     ARFDIQKPLLVYHAQSGALAASRVLQMLGEGKHIALVTDAGTPGISDPGTELVRQARE
                     RLGDGVRIETIPGPSALTAALAIAGVPTHEFVFLGFLPHKKGRQTLFREIADTERTVV
                     FYESPHRIEKALASLAEVLPEERKVVVLRELTKIHESVVEGSAPVVADHFARNQQEVR
                     GEFVVIVAP"
     gene            15669..>16110
                     /locus_tag="VEA36_00405"
     CDS             15669..>16110
                     /locus_tag="VEA36_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28813.1"
                     /translation="MKPRWIGNQGTQLLTRCVSPCAGPWTPETVFDDPAMRSRVEAQL
                     VAVEEARRLASGTKEKREVHANIIRLKDEGEGTILGSHSARTVDKRGQIELRVFSEPA
                     AGIWLPHFHTAVMQLTGRPILCAFSGSHCLLVRFNEACIGVITQE"
BASE COUNT         2839 a         5065 c         5135 g         3071 t
ORIGIN      
        1 gatgatcgtg cccatcatcc ggcccaagtc cttcgtcggt cccgagcgcc tcggagtctt
       61 cgccctcttc tacgattggg ccgccgacgg caagggcttc cccatgatcg ggagcgggaa
      121 caatcgctac caattgctcg atgtcgagga cctctgcgaa gctatctacc tcgcttccat
      181 cctcccgccc gagcaggcca acgatacctt caacatcggg gcgaaggagt tcaccaccat
      241 gaaggaggac taccaggccg tgctcgaccg cgcgggcttc gggaagaaga tccgcggctt
      301 cccggccgcc cccatgatct ggacgctccg ggtgctggaa ttcttcaagc tctccccctt
      361 gtataagtgg gtgtacgaga cggcctccaa ggatagcttc gtctcgatcg acaaggcgga
      421 acagaagctc ggctggaagc ccaagtactc caacaaggac gccctggtcc gcaactacga
      481 gtggtatctc ggcaagcggg acgaattcgc gggcgcaagc ggcgtgacgc accgcgtccc
      541 ttggaagcag ggcgtacttt ctatagccaa gcgcttcttt tagccatgga ccggcggcat
      601 atcggcatcg ccatcgccgc cggtgccttc atcctcctga ccgcgtggca ggcaacgcac
      661 ctgcatcggt atctcgcgcc cgacagcttt acctatttcg cgatggcgaa ggagttccat
      721 gaatcggagc tcctctattc gtactcgggc gtggatcaca cgaccggcat ccatccgggc
      781 tactacttcc tgctcctgcc gctgtatccc ggggttgggg ccgcgcttcc tgcggcgtcg
      841 ttcatcctga atgcgctctt catcctcggc gggttgttcc tcctataccg tgcgctcggc
      901 tttatgccga cggccgtgat cttcctgctc acgctgacga gccaggccgc gggtgcactc
      961 aataacggca tggagtcctc gctcctcttc ttcgcgcttt cgctcgcaac ctatctgtat
     1021 ctcgcgaagg tcgagccggg cgacacccct tcgctcctcc tccttggcgc ggcgcttgcg
     1081 cttgcgctat tcgcgcggct cgatgcgatc ttcttcgtcg cggcgctcta tctcgtattc
     1141 gcataccgga tgctcatgca ggaccgctcg atcaacgggc tcatcgggca cgtacgcacg
     1201 ttcatggccc tgtcgctccc cgtctttgcg gccctcggca tcatcttcgc catcaacctc
     1261 acgtatgacg gcacgctcct cccgctctcg ggacagctga agtcctcgct cccccatgtg
     1321 ctcccgaact gggcagcatc gctcccgctc ctcaagatgt tcatcgccgg catcgcggcg
     1381 atgggggcgt acctcgtata ccgcatgctg aagcgtcgtc cgctccagga actcgttccg
     1441 gcgctcttca tcgggtccat tgccctcttc acgtacaatc ttttctacgt ctcggggatc
     1501 ggcgcatggt acggcgcgct ccccttcttc ggcgcggcgc ttgcgctcgg gctgctcgtg
     1561 catgatctgt ggaccgatgt ccccggtaga taccggcgca tctccacagc actcgtactt
     1621 gttgcctttg cagttgccgt accgcttggc cacgcgaacc gcatcgtgac cgattggatc
     1681 accccgaacc aggcgatcgc cgaggagctc gatcggcagg ccgccgatgg cgaagcggca
     1741 gcggagttca aggacggggt cgtcgcgttc tatagccgcg ttccggtata tagcctcacg
     1801 ggcctcgcga acaaccaggc gtacgtggat gcggtccgca aagggacgct cgaggcatat
     1861 ctggccgagc gcggcatcgc gtacatcgcg cacgggatcg gcgggtcagg gctccaggtg
     1921 ccggaggccg tagagagcat cgaatgcacg aggcccttct atgaaaaaga cggggcgctc
     1981 ctctcccggg tcgcggattg cgccgtcgta cctacctccc gatgaacgcg aggaggcgct
     2041 cggcgcgctt ctcccaggtg tagtcctgga cgagctcgcg ggcgcgagcg gcgcggactg
     2101 cagccgcttc cgggtccgca aggaccgagg cgatgcccgc agcaagcgct gccggatcgt
     2161 caggagcgac gagcgtcaca ctcgcctcgt ccacgacctc tcgtatggag ggcagatccg
     2221 aggcaacgag cggcgttcca gaagccaggt acgcaaagag cttcaggggt gaggtgtgcc
     2281 gggcggagat ggcctcccgg gcggtattcg ggagcacaac cacgtccgct gcggcgagat
     2341 tctgcggaag ctcttcgtac ggccgctgac caaggaagcg gaccttcgga tgccggcgct
     2401 gcagcgtctc cacctcagga ggcttcccgc cgatgatcac gagctcggta tgctcgggta
     2461 gctccgcagc gaggagcgtc tccaacccct tccacccgtc gagtctgccg gcgtacatga
     2521 cgagcggcgc gtcctgtgga agcccaagcc gcctgcgagc cgcctccttg gattcgaccc
     2581 tttcgtaggc agcggcatcg accgcgtccg gcacgaccgc gaccttcgcg gacggtatgc
     2641 caaactcctt gatcaggtcc tcgcgaagcc cgttcgtgat tgcgatgatt ccagtggcac
     2701 tgcgcagtgc aggcgcaagg agcagatcac ggtatatgga atgcgcttcg aggtaaaaac
     2761 gggccgggat gccgagcgtt ttcgcataaa gcgccaggaa gaaaaggagc cgctcctccc
     2821 gaagcaggac gtgtgtgtag ccttcgcgcc ggatgagcga gagatagcgc agcgcaagca
     2881 gggggcttgg cgcgcttccc ataaccttta ccggcatatt gggatttccc gctgagacgc
     2941 cgagcgtaaa atcgctgccg agcctcgccg cgagcgcgcg cgccgtctcc agggtctgca
     3001 cgcggttggc gagcgccgag ggataggtca tcgacgtcgc gtagaggagc ttcatggcct
     3061 gcggtagacc tcgaggatcg ccctcgtgag cgctccgagc gagtgctcct cgtggatgcg
     3121 tgcactgccc gcatcggtca gcatgcggcg ctcttcggaa gagagcgcga gtatgcgccg
     3181 gagcgtctcg gcaagggatg ccggatcgtt ctccttgaaa tagcagggct cgggaacgag
     3241 gtccgcgaat gccgggctcg acacgagcgc gggcgtgccg caggcgatcg actcgagcac
     3301 cgatttatcg taattgccgg cagcggtgag gttcacgctc accgcatgcg ccgcgtacaa
     3361 ggcaggcgtc tctgcgttcg ggaccgggcc gtagaaggtc acccgtccgc cataaatgag
     3421 gtccttgaac tcgctccgga gcatcgcttc atacgagggt tccgcaggac cgacgagcgt
     3481 taccgtcgcc ccaggaacgg aacggagcgc ctcaaggagc acgtgcacgc gcttggacct
     3541 gctcacgcgg ccctggaaat aaatgctctt tagtcgcacg tctacgccct gcggtcggaa
     3601 gacctgcgta tcgatgcctg ctggcatctg ctgcgacttc ttcgtccctg cgcttgcggc
     3661 atagggcgag gtatggagga gcgcatcgac gaagtacgac gcgagcttga gcttccatcc
     3721 gccatggtcg tggttgtacc agagcgcgac cttcttgccg cgccagaacg ggaagccgag
     3781 gaggacatac tcgggattca tatgcacgaa gacggcatcg taggtgccat cgagctctcg
     3841 cagatggtcc tggaacgtcg cggcatacgt aagcttcccc cgcctgccgt tctccttccc
     3901 gagcgagcgg accgctacat tccctggcaa cgaatgctcg ccgaccgaga gcgcgataac
     3961 ggtcacctgc tcgcattcct tcgcaagggc agcgacccat gcatggaaga agccgagcac
     4021 cggatcgctt cggtccagta tctgcgtcgt gatgaggagc tgcataggtc aggcggaggg
     4081 cccgtagacc ccatcgacga tctccttgtc cttcccgctc tgcgcaagga tcgcgcggtt
     4141 ggccgcttcg gccgcctgga gcgtgcgcac cgacgggtga tcgcttccga gctctttctc
     4201 cgccatatgc cggagcgccg cgaaatcctt cacgaagcag tggccgcccg cgccgcgccc
     4261 tgctttatgc acggggttgg aatacgtatg ggagatgagc ggatcggccc ggagcgcctc
     4321 gccgatccgc tcccaatcgt acccgagctt ctgtgcgagg tcgtacatga aattaaagaa
     4381 gacgatctgg gtatacccac tgccgttatg cgcgtacttc acaagctccg cctccgtgct
     4441 cgtgataatg gaggcatagg gcgccggcgg cagcatcgcg atcacggtct ccgcagcttc
     4501 ccggtgcagg tcgtcctcca ccgcgacgcc catgatgttg gcgaacgggt tcgcgacgtc
     4561 ctccgcagcc cggacctcgc gcaggaactc gggcgaatag atgacgatgc ggtccggata
     4621 ctgcttctgg atatcaacgg tcctgccggg cagcacggtc gatttgatca cggcgatctt
     4681 ccctaccccg acgagcccga ccgccccacg cacgatcgag tcatcgaaac ggatgccgcc
     4741 cgcgccgtcc ggcacggtcg gagtcggcac gccgatgaag acgacgtcgc agtccttgat
     4801 gagctcctta ttctgtacgt acggctcctc gagcgcgtac cggacgacgt cgtacccgcg
     4861 cttctcgaag tcgtcggcgt agttcttgcc gatgtacccc tggccaatga atccgatccg
     4921 catactaggg gcgtgcgaca aacgcgaggt cgaaggcgaa gaagttcggg gagagcttcg
     4981 cgagccgggt gtaccgcccg agcccagtca cggtcacctc ccggaagtgg cgcgcaagaa
     5041 gcgcctcgag ctccggcgcg ctgaactggt tgatatgcgt cgggtccgag agtggcgtgt
     5101 gcttcttcgt gccgcgcagg tagcggagcc ggttcttcag gctgaatgcg ttgggtacgg
     5161 agcccacgag cacgccgtcc tccttcagcc ggctccggat cttgcagacc acctgctccg
     5221 ggaaatagag gtgctcgagg atctcgcccg cgaccaccgc atcaaattga cgatccccga
     5281 gctcctccca atctcccaag agatccatct ggatcgcctc gatgccgagc tcgcccgcgc
     5341 gcgcgagcgc ctcgcggtcc acatcaacgc ccgtgaccgt attcccttcc atgaaggtgc
     5401 gtgtgagtgc tccgtctcgg cagccgatat cgaggatgcg cttccccgtc ccgaccgcct
     5461 cacggatgaa ggcgccccgg tcgtccttga ggatgctgaa gccgggcgcg cgccgcgcct
     5521 tatggtgcgc ctcgtagatg gtctctgcct gcgacatagc tcttgttcga gaggatacca
     5581 cgtatggcta tgctccctct aacctagcgg ggtgccgcga gggtgcgggc gaggtcctcg
     5641 gcgatcctcg cgggctggtc cccgattgat gcgagatgcg ccgcagcctt gcgctccgcg
     5701 ttccggacga gcgattgccg ggcctgcacg tcctcgacga gccccgcgat atggaccgca
     5761 agcgccgcgg gatccgcgac gggagccacg agcacgtcct cgtaccccgt gaatacctcg
     5821 ccgacgatgc ccacatcggt cgtgatgatg ggcacgcggg cgcgcgccgc ttcgagcagg
     5881 gtgaggctgt atccttcgta ggcgctcgtc tggatatagg cctgcgcgct ctgcatgagc
     5941 ccgagcgcat cgcttcgcca gccgaggaac atgacgcgct caccgagccc gagcatgcgt
     6001 gcgcgcttca tgaggcgccg ccggctcctt ccatccccga tcacgatgag gccgaccata
     6061 ggatacgtgc gcgccacccg cgcgagcgca tcgatcgcat cctcgatgcg cttctcgggc
     6121 tcgagccgcc cgaccgtcac gaaagcgaag gagaacgggt ggggcgggag cgcgaccctc
     6181 ggcgggagcg attcgggacc ggcaatcggg atgacgctga tcggtcgatc cccgtaccgt
     6241 ccgatgaggg acgccttcac gcgctccgag acggcccgga tgccatcggc ccgtgggatg
     6301 atccgatccg cgatgctgcg ccgtatcgta ttcaaaacag gcatctgcac gtgcgccgcg
     6361 cgaagcatcg tccctttcac gaaccagggc gagcagaagt cggtatggac ctggagatgg
     6421 agcttcgcgc tcgtgccctt cttcgcttga tacgccaccc agccatgctc gaaggggtcc
     6481 tgcgcggaga cgatctcgat gccctgttcc ttgatgagcc tccgagcttt gccctgcatc
     6541 gcgtggagcg ccgcgagctt cccgccgtcg acggcatgga ggtgaagtgg accgtcctcg
     6601 aggtccgtcg catgcgggcc acgcgagagc acatggagcg tgccaatctc cgcagcatac
     6661 cgtttaaggc gcgtgcgtgc gggactctcc ggcgtgaaaa tggtcggatc cgagccgatg
     6721 aagagcgcct tcatgctggt catggtaccg caaggagcgt cctgaattcg gccgccgcat
     6781 cttcccaggt acgcctgcgc cgggagctcc tcatacgtgc tacggcatcc cggtagcctg
     6841 ccggatcgag gagcttctcg atcgcggcca cgatctcatt cggatcccgc gtatccacga
     6901 acatgccgct ctcggggtag agctcgtaga ggccggtatc cttggtcgtg atgaagggct
     6961 tcccgtaggc gatggcttca agtacggcgt tcgggcacac atcgctgatg gacgggagga
     7021 tgagcgcata ggaggcagcc agcgcctgcc ggtacgcctc gggcggacgg ggtgtggtat
     7081 cgagctccgc ctccggatgc cgcatctgaa tgttcggcca gagctcggcg aggagatcgc
     7141 cgttcttgag gtacggaccg cgggacgctg caaggaatac cttcgaggaa gtatcggttg
     7201 tcgcagcctc catgggctcc ggcaccccgc tctcgacgac gcttgtggac cttcccgaaa
     7261 gattgtatgg ctccatccag atgcgctcct gccaggcggt cgtaaacgcg atgtggtcaa
     7321 cgaacgcgag cgtgaagcgc gtggcctcat ggatgagatg ctcgcggagc gtgagcacgc
     7381 gcggcatcgc atagaactcg gagagacgga tcggttcgcg tacgcggttc gcgtgcatct
     7441 cccacaggaa atctccgccg acccgaagga cggagcgctt cttcaggagc cgcgccaatg
     7501 gggcgaccgc gagccccgtg gataccggat cgagaaggag gacggcatcg gcccatcgta
     7561 cggtcgggat cgcccggatg aagagcacga gctgccgcac tccgatgggc aggcgcttct
     7621 cgaaccatga gggcgtgatc acccggacat cgtccccatg ctcctcatac acgcgggaaa
     7681 gctcgcgcgc gacggttgcc ggccccccga tatcctgaag cccgatggga gacgcaataa
     7741 gaagcttcat ggcttagcga agcaggcgcc ggaagagact ctgcatcgat gcggcgaccg
     7801 tattccagtc atagtgctcg ataaccatgg ccttggcggt cgcggttacc cgctgcacct
     7861 tctcgggatt cgcgatgatg tcccgcacag ccccagcgat ctcctcaggg ctatccttcg
     7921 agaccgccca gccagtcggt tccttatcgg gattgcgctt ctcgtcgaag aggaagtccg
     7981 cgatgccgcc ttcctgcgtc gcgatgaccg gaagcccggc ggccatcgcc tcgacgaagg
     8041 aattgcccat gccctccgag cgcgacgggc ggatgaagat atcacaggcc ctgagcgcaa
     8101 ccggcatctc tgcatgcccg atctgccccc ggaactgcac gcggtgcgca accccgagct
     8161 ccgtggcgag tcccttgagc atctcttcgt ccggcccgat gccgaagacg atgaacgaga
     8221 catgctccgg aaggagcggg agcgcgcgga tgacatcgtc caccgcgttc ttatgcacga
     8281 ggcgcgaggt cgtgacaagg aagacatcgc tctccttctt gccgagcttc tcctgcatgg
     8341 cgcgcacgtc tgtgtctggt atcggctttg cgaaatgcgc cgtatccacc gcgttcggga
     8401 taacgatacc ttcgcccctg aatcccatcc gcttccccca ccgcaggagg aaggtagaga
     8461 ttgcctggag ctcatcggcc ttcgtaaagc cctggcggaa gagcggcatc acggggcgca
     8521 tgagcccctc gatgtattcg ggcggatcgc cctcctgcag cgtcagtagg taccgcacct
     8581 tcggattgag cgccttgaag attcctgccg ggatgccggc ggagtgcgcc atcatcgccc
     8641 aggtgccgtc gtagcggtaa cgccgatgga gccagagcgc ctcccacgcc gccagaagct
     8701 ggtacacgtg cttgttgagg tggagcggga acttcttgag gtccccgatg cccggatgcg
     8761 ggaccccgaa cccgatgcgg tgcacggtga cgttcccgag gcgctccgtc ttcgggatgg
     8821 tggaatcgaa ccggcaggta acgaggtgga actcgacctc cccagcgggt atccgatccg
     8881 tgatctcctt gatcgcgacc tccgctcccc cgacgaaggg gtagtactgc atatcgaata
     8941 tgaggacctt cttcatgagc aaaagcctag cgtgattgcc acaaaaggaa aaggcggccg
     9001 ctcgcgcggc cgcctgacca tagttccctg ccgcgccctc gttagacggc ggcgagctcc
     9061 ttgccgacga cgccggccag gtactgcggg aaggtcgcga agaagatcat gaggatcgaa
     9121 ttggccgtgc acatgcggta cacggcgccg tcggcatcga cgccgctctt gatgcggctg
     9181 aagagctcct ccaccgtgac gtattcagcc gagaagatcg gctcgttgcg gtcgaacttc
     9241 agctcattga tgcgcttcag gttgacctgc acgaaccaca ggtccgacca ggtgtcgacg
     9301 aaggttggat tcgggttgtg gtaggggtag gccggctgga acatgctaag aacatggcag
     9361 ccgccggtct cctcgtcggt ctcgcgttgc gcagcctctt cggccgtctc gccgatgatc
     9421 ttctcgagga agcccatcgc gatctggccg aagacgaccg gcgcgtggcc gtcgacgcca
     9481 ggctggagcg gatcattggc gtgcggacgc ggctgacgga tgatggcgat cctgacggtg
     9541 ccgtcatcct caaccccgta cgcgacgatg ttgacgttcg gcgcctccga atacagaggg
     9601 cgatcgaaat tcggcgtgcc gtccgcatgc gggacgacag ccgggcgcac ggcgccgaag
     9661 cgggaggtga cctcccagtc cgtctccgcc ggcatctcct cgccgttcac ccggagcttc
     9721 cacccaggtt gccagagcgg cttgctcggg tcttcggcgt ggattttggt ggctgcggca
     9781 agcgcaggta cgagtcgcag ggtcgcgctc atgattttct tcctcaaagg acaacggcgt
     9841 gctgccgttt cgtagagcac tacagcatac taatgacaaa aaatcaagca cctcgcgagc
     9901 tgctgtcccg catgcgcttc tcctgctgga gtttcgcgag gaacgagcca taggcgaagc
     9961 ggatctggtt cgcgaacgca tcgccctcgc caagcgagcg gcggtcgcgc atacggcggg
    10021 caagcgacgc gatcgtgcgg ctctgttccg gcagcgatgc atacacctga tcggcctgtc
    10081 ccatcaccaa ccggaggaag agccgttcgt accaggagag gcgctggtct gcgagcgtca
    10141 cgaggagcgg aagcgtagcg cgacggcgca ccgcaagcgc cgcgagcgtg ccgtagcttg
    10201 ccatgacgga ccaggagaag aggtagtcgt gctccttcgc gagccgatgg ccgatggcag
    10261 cgccccagaa cggcaggaga tacttatcga aacgcgtccc ccaccccacg tggtacacgc
    10321 gtgcattcga tccgaaggtt gccgtaccgc tcgcctccgg cgtatgcatc gcggtcacaa
    10381 tatcgaactc gacgtcgggc atcttctcca tgaggagcgc gagcgctcgc tcggcatccc
    10441 ccatgatggg atggaacgtc gtggtgaaga cgaggatgcg attccccttc ggcgcgctcg
    10501 ggctactggt ccgcttgatt tcttcgatgt acgccgcgag gctgcgctgc ggcttccagc
    10561 cgagcgctcg tgccttctcg gtctcgagca gggacgccat gcgattgccg gagcgctctg
    10621 gcagcatgac gatatcctct gagaacattc tcgccacctc gaggatggag taggcccgct
    10681 catcgccgag cccatagtca tcaccatggc ccttctctcc aacgaggaga aggccgtcga
    10741 cgatatcgtc gacgtgcgtg aaattacgtt cctgggtccc gggcgctacg agtccgatgg
    10801 gacgacccgc aagatagctg cgcttgaaga tctcgatgac ggtaccgaag gcgccagcgc
    10861 gctcgcgtcg tccgtaaacg ttatagaaat aggtaatcgc ataatcgatc ccgtaccagg
    10921 cgccatagtt gcgcacgagt tcggtattgg tcgccttcgc ccacgcatag gggctttgct
    10981 cgcggccgat gcccccgtcc gcgaacttcg tggagctacc tgcatagatc agcttcgcgc
    11041 cccgccgacg cacgaactcg agcacggcga aggtgccggt cttattgagg tcccagagcg
    11101 cctcgatgtc ttcgaagctc ttctccacgc gcgagtattc cccgaggtga tacacgaggt
    11161 ccggggcctc gggaacgagc tgctcgatgt ccttcgtatg cccttcgcgg tattcgacgc
    11221 cagggacatg attctcacgg ctgccggtga aataattgtc gagcgaaatg acccggtacc
    11281 ctgtcttcgc gagccgttcg cataggtgcg agccgatgaa cccggcgcct ccggtaacga
    11341 ggatgatctt gctcatgtat acggctacga tacggcagga gcgcaagggc ggcaaacggg
    11401 cccccgcggg atggcgactc ctcgtccatc ccgcgggggc aactatgcgt ttgccatggt
    11461 cctcgtgatt aggtcttcca atccttcagg tattaatacc agcaccgcga ggacaaggtt
    11521 gcactagaac ttgtccttga agatatcgcg gagcacgtcc tcgggaacct cgaatacgga
    11581 ttgcgtattc ctcgtatgct cgggttgctg tggtcgtcgg ttctcctgtc gtggttgctg
    11641 ctgttgctgt tgtttgggct gcgcctgttg ggtgtggggt ttcggcgccg gctgctcctt
    11701 cttgaccacg tccgcgagcg cggacttgag cgattcctcg gattgcttcg tcttctgctc
    11761 cttctcggtc ttcacgccgg cgatggtgct cttcaagatg gccttgagat cgtcggcggt
    11821 gcgagcgctt gcggcaggcg cgccccgtgc ctcttggcgc ggcgcgggcg cgcggttctc
    11881 gcggcgcggc tccgcaggac gcgggcgctg attgcctgcg ggcgggaggt agcgcttggg
    11941 cgatggctcc ggggccgcct cgtccggatt gagcgagagc tcgtcataga tggacttctc
    12001 gatgcctgcg cgaggattgc cgaactgcgt ccgcgacgcc tcgatgacct cctggcgcca
    12061 cgagcgcggc ggccgctcga tgggcgggat ggtgacggcc gagaagggcc gcgacccgat
    12121 gccgtcgatc atgagcgaga ggtagatctg cgcgaacccg aggttcacga ggtcctccgg
    12181 ggtgaactcg ggggcgaaca ccacttcgag cttctcggca tcgaagggcc cgacccggaa
    12241 cgcgatcgtg gtgccgacgt tgccgaagac ggccgccgcc acctcctcct ccatctgctc
    12301 gacgtactga tgcgcgatga tgaggttcaa cttgtacttg cgcgcctctg acaatatctc
    12361 cgcgaaggtc tcgttcgcga agttctggaa ttcgtccacg taaaagtaga agttgggagc
    12421 cttggccagc tcgtgctgcg gcagctcggc ccggctcatg gccccgagga agaggcgcgt
    12481 ggtgagcatg gagcccagga ggcgcatgtt cacatcgccc acctgcccct tcgagaggtt
    12541 gatgagcagg atcttccgct gatccatgag ctcgcggata tcgaaggagg acttcggctg
    12601 cccgatgata ttgcggatga gcgggttgct cgtgaactgc ccgatcttgt tctggatggc
    12661 gggcgtcgct tcctgcgtat accggtcggt gtacgtcatg aactccttca cccagaagtc
    12721 cttcaccacc ggatccttga tattctcgac gaccttcttg cgatatacct tgtccgtgta
    12781 catgcggttc accccaagca gcgtggcgcc ggggtattcc aggagcgcga gcaaggtatt
    12841 cgtgaggatg tactccatgc gggcgctcca ggcgtcctgc cagatcttct tgaaggtggc
    12901 catgaggccc gaggtcacga ggtggcgctt gtcgtagccc acgtcctcca tgacgttgaa
    12961 ggcgatcggc atatcgaggt cgaagggagc gaagtgcacc acatccttga tccgatgctc
    13021 ggggatgaaa tcgagcaggg tctcgatagc gctgccgtgc ggatcgatga aggccatccc
    13081 ttccccattg cggatatcct ggatggccat gttctcgagg agcgtggatt tacccatgcc
    13141 cgtcttcccg atcacgtaca tgtgccgagc gcggtccttg gccttgatac cgaagggggt
    13201 cttcttgccg cgggaatcgg tcgcggcgaa atacgtcact cgttccggat tctccatatt
    13261 tgcatcatta taccgcgaga taaagaagac gacccgctgg gggcgggccg tgatctgatg
    13321 ccgtgcggcg cagtgactct tcttagcgct tacgcacgtt cggagtcatc ggtagcgctc
    13381 ttcgcgttct tcggcgtcga cgagctggga gcgcagccag aagagccaga ggcccttcag
    13441 gagccactcg atcgggcaga cggtctggta tccctccgcc aacctgatcc agttcttgta
    13501 gcgccaaatg tgcgcgcgag cctccgccgc cggtgtgggg ggatagaatc ccatggccgt
    13561 gactcctcgc tgactgcgtc taccctacca ttattcagct tggatgtcca gctactggta
    13621 ctggatgtac atcggccgcg gcgtgagctt gaggaggtca gcgggggtca gcaggcgctc
    13681 cgacggcggc ttcatatcgt tcttatagaa gatcttgaag ccggtgaact ctaccggctc
    13741 cggatagatg aagctcttgt acgtattaat cttcttcgcg ggcggccccc acccgtccat
    13801 gaccatgacg atctgcacct cgggccgcgt ctcgatgagc tggtagtcgg ttaccatgcg
    13861 ctgcgtgaac cggtgcacga cgaggatctt cggggggagg tcatgcttcc gtacgagctc
    13921 cgcgaggtat gcggaggcgc ggttgatgtc gcttgccgat accgtcccga tgaccgttcc
    13981 cggcgcctcc ccgcccttca tgttgaactc gggatcgatg gcgaggtgca catccggctg
    14041 cacgaggtat ttctcaaatg cagtgatctc gctctgcagc ggcgcaagcc ctgcctgtat
    14101 ctcgagcacg gtgatgccgt tgactttgcg ggccatggta attgcccgct ggatgtgctc
    14161 atcgggcatc cggaggcgat acgtcttgtc cgcttgtggg tgcagctgcg cggtcgtggc
    14221 gatgtaatcg atcgccggta cgaccttcgt ctcggggtcc gccgcttccc atttcgccac
    14281 ctcgcccttc aagcgcgcga gcacggtcgc ttcatcgaac tcgccgagga tgcccattct
    14341 cgtggaatag aagttgccgt agtaggcgac gacgcgcttc atcggcagga tggcgccagc
    14401 ctttgggtag ctggtcttca ccggccagag acggcttgag gtcgcggtgg tggaggcgta
    14461 gttggccatc cgctcgagct gcgtatcgta gagcttcgtg tcgagggtgg gcttcgcggg
    14521 cgggatgagc ttcgcgacca cgcttgtggt cgcagtcttg gcgtagttct ggtgcatcgc
    14581 gatggcgtac gggatgccgt gcaccgtcac cgcgatgaag acaagcccga atccgacaag
    14641 gatcgcgacg ggattacgaa agagccgatg gaggtgcttc atgggtgatc gcgcgacgcc
    14701 ggcggcgcag ccggagcgtg aagccgatcg ccgcgatgag gagacccgcg gcgacataga
    14761 accaggtgag gagggagagc ggaagcccag tgaacggcga gacgacgatg aggatgccga
    14821 gcaccatcag gaggctttcg cgggacatag gcccagtata ccgcgccctc ccctacggag
    14881 cgactatgac gacgaattcg ccccgtactt cctgctgatt gcgcgcgaaa tggtccgcga
    14941 cgaccggagc actcccctcc acgaccgatt cgtggatctt ggtgagctcc cggagcacca
    15001 ccaccttccg ctcctcagga agcacctcgg caagggacgc gagcgccttc tcgatgcggt
    15061 gcggtgactc gtagaacacg acggtgcgct cggtgtccgc gatctccctg aagagggtct
    15121 gccggccctt cttatgcggc aggaagccga ggaatacgaa ctcgtgagtg gggacacctg
    15181 cgatggcgag cgcggccgtg agcgcggagg gtcccgggat ggtctcgatg cgcaccccgt
    15241 ccccgagccg ctcgcgggcc tggcgcacga gctcggtgcc ggggtccgag atccccggag
    15301 tccctgcatc ggtcacgagc gcgatgtgct tgccttcccc gagcatctgg agcacgcggg
    15361 aggcggcgag cgccccgctc tgcgcgtggt atacgagaag cggtttctgg atatcgaagc
    15421 gggcgaggag cttggcggag acccgggtat cctcgcaggc gatggcgtct gcggccttca
    15481 gcgtctcgac cgcgcggaag gtgatgtccg cgaggttgcc tataggagtc gcgacgatgg
    15541 agagggtgcc ctgcatgagc gcatgctagc agatgccgat tgacgtgccc atggggcagg
    15601 cgtaaggtgt gggctggctc attaagtaag taacgggaga gagtgtattg aacagcacaa
    15661 caacagccgt gaagccgcgg tggatcggga accagggtac gcagcttctt acccgctgcg
    15721 tctcgccctg cgcgggacca tggacgccgg aaacggtgtt cgatgacccg gccatgcgca
    15781 gccgggtaga agcgcagctg gtagccgtgg aagaggcgcg gaggctcgcg tccggaacca
    15841 aagagaagcg ggaggttcac gcgaacatca tccgcctgaa agatgagggc gagggcacga
    15901 tcctcggctc gcactcggcg cggaccgtcg acaagcgggg acaaattgaa ttgcgcgtct
    15961 tcagcgagcc cgccgctggc atctggctcc cccacttcca cactgcggtg atgcagctca
    16021 cgggcaggcc gatcctatgc gccttcagcg gctcgcactg ccttctggtg cgcttcaacg
    16081 aagcctgcat cggggtcatc acgcaggagt
//
LOCUS       DASPKG010000009         5590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_555605, whole genome shotgun sequence.
ACCESSION   DASPKG010000009 DASPKG010000000
VERSION     DASPKG010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555605"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1170
                     /locus_tag="VEA36_00410"
     CDS             196..1170
                     /locus_tag="VEA36_00410"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HYF28814.1"
                     /translation="METSTTGSRTTPKDFFLWLLAVIALYGSIISFIALLFEYINYSF
                     PDALAGYADPYGGAVRFSMAALIVLVPTTVVIMHYLRRIIIEEPGKAQIWVRRWAIGL
                     TLFIATLTILIDLVTLINTFLNGEITVRFGLKVLTVLAVAVFIFFHTLLDMKGYWIKH
                     GRRAHLLGGFTILVSLIAVVAGFFIVGTPGAARDVRFDIRRVNDLQMIQGQVLSYYQQ
                     KQTLPDSLSELEDPLAYFTLPADPETNADYVYKKTGDLSFELCAVFAREGKDLAGRGG
                     FGRDIAVSYPYPGPDGAMENWQHGEGQQCFERTIDPERYPPFEKPILR"
     gene            1174..4026
                     /locus_tag="VEA36_00415"
     CDS             1174..4026
                     /locus_tag="VEA36_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYF28815.1"
                     /translation="MARKGYDHRKVEEVAQKKWAGLDLYSTDLRDEEKEPYYLLIEFP
                     YPSGDLHIGHWYAYVVPDIYARFKRMQGRNVLFPIGFDAFGLPAENAAMKNGADPRRW
                     TEENMARMREQIRSMGTSLDWSKEVVTCDPSYYTWTQWLFLKFFEQGLAERREAPVKW
                     CPKDRTVLANEQVIDGACERCGTEVEEKQLTQWFLKITKYADRLLRDLDALPWREDIK
                     EAQRAWIGESEGAKLTFSLETAATAASLGKAEVFTTRPDTIFGATYLVLAPEHPMVEG
                     LIDFVQNPAEVRAYRETTSRKTERERSENKDKTGVALAGVVAVNPASGERIPVYIADY
                     VLASYGTGAVMAVPAHDERDFEFARRFRLPVRHVIAPRRVDERNPHLPDKELVKRNII
                     VGIVRNPKTGNYLGLSWKKQGWISFITGGIEEGEDMVETARREIAEETGYTNLRYVRT
                     LGGPVHVEFYAAHKDVNRIAYANHLLFELEDETRAEVSKEEQDLHEALWLDEGKLTRD
                     QMDHAQMDLILERLRSDDCAYTDDGVLINSGTWTGRDNREVIWEMVESIGGERVKNYR
                     IRDWLVSRQRYWGCPIPIVYDPEGAAHPVPPEHLPWLLPEDVDFTTSDAAPLASSQEL
                     KERVTKLFGEGWTPECDTLDTFVDSSWYYLRYLDSKDEGDFSDQAMLKKWLPIERYSG
                     GSEHTTMHLLYARFFHKALYDLALVPESEPFVERYNRGIILGPDGQKMSKRWGNVVNP
                     DDVVKQYGADAVRVYYAFMAPYNESGHYVWSDKGVESARRFLDRIASLADTVGDADAS
                     EALMQMIARTARKIAEDAERFKFNTAVAALMVLVREFEGQEEVSRTAYHDLLRLVAPF
                     APHLAEHLWEETGGERSVHEASWPEGAIAASAEATVIVQVNGKKRGELLLAVDAGEEE
                     AVAAARGLEAVEKALAGQEIAKTVYVPGRILNLVVV"
     gene            4073..5170
                     /locus_tag="VEA36_00420"
     CDS             4073..5170
                     /locus_tag="VEA36_00420"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HYF28816.1"
                     /translation="MAKKPATTARKGAKAAPKASAKRPAAKLSFDSAVLVKRLLAEVP
                     ERAREVLVYRFGLGTSAERETLEAIGERWGITRERVRQIEASGIEAVRNSKSFRESAS
                     AFGELCAYVESLGAIVPEETLLKGLAGDEKAKNWFRFLLVVGSEFFRERETDDFYARW
                     HVDHRTADAVHTALTKLYSSLDDEEVLSEGQIINRFLEELKGVNSAYQQEEILKRWLS
                     ISKTISSNPLSEWGRAHAPAIRTKGIRDYAYLVIKQKGEPMHFADVAKSIVDIFGKKA
                     HVATTHNELIKDSRFVLVGRGLYALAEWGYSPGVVRDVIRDVLKKKPLSRDEVIAEVK
                     KVRYVKDNTILVNLNDSRYFKRQKDGKYAAV"
     gene            5237..>5590
                     /locus_tag="VEA36_00425"
     CDS             5237..>5590
                     /locus_tag="VEA36_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28817.1"
                     /translation="MSGGPGSPLPFLDKIEKLFGFGHEQGFAFTARPFIIAGLVAVLT
                     FLIISPAQTLRNFEFLVFTSPIWLSAVLVRFAIYRFVEANRMQFLARQEYVLLEIKLP
                     RDIKKSPLAMETVLTN"
BASE COUNT         1025 a         1757 c         1806 g         1002 t
ORIGIN      
        1 acctgggaca cggagctgat catggtccgc gggcagctcg cggtccgcga ttgccgggtc
       61 atccgggcga gcgtgggcgg cgtctttccc tccgaccact acttcgtgtc cgcagatctt
      121 tcctacgcgt gaacgacggc ccgcctgtgc actctgcagg cgggtcagtt ctttttatgg
      181 tataatccgc gtactatgga aacatcaacc acgggctcgc gcacgacgcc gaaggatttc
      241 ttcctctggc tccttgccgt gatcgcgctc tacggctcga tcatctcgtt catcgcgctc
      301 ctgttcgagt acatcaacta ctccttcccg gacgcgcttg cgggctacgc cgatccctat
      361 ggcggcgcgg tgcgcttctc catggcggcc ttgatcgtgc tcgtgccgac caccgtcgtg
      421 atcatgcact acctgcgccg catcatcatc gaggagccgg ggaaggcgca gatctgggtt
      481 cggcgctggg cgatcgggct cacgctcttc atcgcgacct tgaccatcct catagacctg
      541 gtcacgctca tcaatacgtt cctgaatggg gagatcacgg tgcgcttcgg gttgaaggtg
      601 ctcacggtgc ttgcggtcgc ggtattcatc ttcttccaca cgctccttga tatgaagggg
      661 tactggatca agcacgggcg ccgggcgcac ctactcggcg gcttcaccat cctcgtctcg
      721 cttatcgcgg tcgtggctgg gttcttcatc gtggggacgc caggggccgc ccgcgacgtc
      781 cggttcgata tccgccgcgt gaacgacctc cagatgatcc agggacaggt cctctcgtac
      841 taccagcaga agcagacgct tcccgattcg ctctccgagc tcgaggatcc gcttgcgtac
      901 ttcacgcttc cggcggaccc cgagacgaac gcggactatg tctacaagaa gaccggcgac
      961 ctctccttcg agctctgtgc ggtcttcgcg cgggaaggga aggacctcgc cggccgcggc
     1021 ggcttcgggc gcgatatcgc agtctcttat ccgtaccccg gccccgacgg tgctatggag
     1081 aactggcagc acggggaggg ccagcagtgc ttcgaacgca ccatcgatcc cgagcggtat
     1141 ccgccgttcg agaagccgat cctccgctaa cgcatggccc gcaagggata cgatcatcgg
     1201 aaggtcgaag aagtcgccca aaagaagtgg gcggggctcg atctgtattc gaccgacctc
     1261 agggacgagg agaaggagcc ctactacctc cttatcgaat tcccgtatcc ctcgggcgat
     1321 ctgcacatcg ggcactggta cgcctacgtc gtccccgaca tctacgcgcg cttcaagcgc
     1381 atgcagggga ggaacgtgct cttcccgatc ggcttcgatg ccttcgggct ccctgcggag
     1441 aacgcggcga tgaagaacgg ggcggatccg cggcggtgga cggaggagaa catggcgcgc
     1501 atgcgcgagc agatccgctc catgggcacc tcgctcgatt ggtccaagga ggtcgtcacc
     1561 tgcgatccct cgtactacac ctggacgcag tggctcttcc tcaagttctt cgaacagggc
     1621 ctcgccgagc ggcgcgaggc tcccgtgaag tggtgcccga aggataggac cgtgctcgcc
     1681 aacgagcagg tcatcgacgg ggcctgcgag cgctgcggga cggaggtgga ggagaagcag
     1741 ctgacccagt ggttcctgaa gatcacgaag tatgcggatc gattgcttcg ggacctggat
     1801 gcgctcccct ggcgggagga tatcaaggag gcgcagcggg cgtggatcgg ggagtcggag
     1861 ggcgcgaagc tcaccttctc gctcgagacc gcagcgacgg cagcatccct cggcaaggcg
     1921 gaggtcttca cgactcggcc cgatacgatc ttcggcgcca cctacctcgt gctcgcgcca
     1981 gagcacccga tggttgaggg attgattgat tttgtacaga atccggccga ggtgcgcgct
     2041 taccgcgaga cgacatcccg caagaccgag cgggagcgct cggagaacaa ggacaagacg
     2101 ggcgtggcgc ttgcgggcgt ggtcgcggtg aatcccgcga gcggggagcg cattccggtc
     2161 tatatcgccg actacgtgct cgcctcctac ggcacgggcg cggtcatggc ggtgccggcg
     2221 catgacgaac gggattttga gtttgcgcgc aggttccgtc tcccggtccg gcatgtcatc
     2281 gcgccgaggc gcgtggacga gcgcaatccg cacctgcccg acaaggaatt ggtgaagcgc
     2341 aacatcatcg tcggtatcgt ccgcaacccc aagacgggga actacctcgg gctctcctgg
     2401 aagaagcagg ggtggatctc attcatcacc ggcggcatcg aggagggcga ggatatggtg
     2461 gagacggcgc gtcgggagat cgcggaggag acgggctaca cgaacctgcg ctatgtgcgg
     2521 acgcttggcg ggcccgtaca cgtcgagttc tatgccgcgc acaaggacgt gaaccgcatc
     2581 gcctacgcga atcacctgct cttcgagctt gaggatgaga cgcgagccga ggtatccaag
     2641 gaagagcagg acctccatga ggcgctctgg ctcgacgaag ggaagttgac gcgcgatcag
     2701 atggaccatg cgcagatgga cctcatcctc gagcgcctgc gcagcgatga ttgcgcgtac
     2761 acggatgatg gggtgctcat caattccggc acctggactg gccgtgataa ccgcgaggtc
     2821 atctgggaga tggtcgaatc gatcggcggg gagcgcgtga agaactaccg catccgcgac
     2881 tggctcgtct cgcgccagcg gtactggggc tgccccatcc ccatcgtcta cgatccggaa
     2941 ggcgctgcgc atccggtgcc acctgagcat ctcccctggc tcctcccgga ggatgtcgac
     3001 ttcacgacgt cggacgccgc accgcttgca tcctctcagg aactcaagga gcgcgtgacc
     3061 aagctcttcg gcgaagggtg gacgcccgag tgcgatacgc tcgatacctt cgtggactct
     3121 tcctggtatt acctccggta cctcgactcc aaggacgagg gggatttctc ggaccaggcg
     3181 atgctcaaga agtggctgcc catcgagcgg tactcgggcg gctccgagca caccaccatg
     3241 cacctcctgt atgcccgctt cttccataaa gcgctctacg acctcgcgct cgtgccggag
     3301 agcgagccct tcgtggagcg gtacaatcgc ggcatcatcc tcgggcccga cggccagaag
     3361 atgagcaagc gctgggggaa cgtcgtgaat ccggacgatg tcgtgaagca gtacggcgcg
     3421 gacgcagtcc gggtctacta cgccttcatg gcgccctata acgagtctgg gcactacgtc
     3481 tggagcgaca agggcgtcga gagcgcgcgg cggttcctcg accgcatcgc gtcgcttgcc
     3541 gacacggtcg gggatgcgga tgcatccgag gcgctcatgc agatgatcgc gcgcacggcg
     3601 cggaagattg ccgaggatgc cgagcgcttc aagttcaata cggcagtcgc ggcgctcatg
     3661 gtgctcgtgc gcgagttcga agggcaggag gaggtatccc gcaccgcgta ccatgacctc
     3721 ctgcggctcg tcgccccgtt cgccccgcac ctcgccgagc atctctggga ggagacgggc
     3781 ggcgaaagga gcgtgcatga agcgtcatgg cccgaaggcg cgatcgcggc gtctgcggag
     3841 gccacggtca tcgtgcaggt gaacgggaag aagcgcggcg agctcctgct cgcggtggac
     3901 gccggcgagg aggaggcggt cgcggcggcc cgcgggctcg aggcggtgga gaaagcgctt
     3961 gcggggcagg aaatcgctaa aacggtgtat gtaccgggcc gcatcctcaa cctggttgtg
     4021 gtgtaatttg acgcttcaac ccccctctgg tataacaaga ccacataaat atatggcaaa
     4081 gaaaccagct accacggccc gcaagggcgc taaggctgcc ccgaaggcgt ccgcgaagcg
     4141 gccggcagcg aagctttcct tcgatagcgc ggtgctcgtg aagcgcctcc tcgcggaggt
     4201 gcccgagcgc gcgcgggagg tgctggtata ccgcttcggg cttggtacat cggctgagcg
     4261 cgagacgctc gaggccatcg gcgagcgctg gggcatcacc cgcgagcgcg tccgacagat
     4321 cgaggcgtcc ggcatcgagg cggtccggaa ctccaagtcc ttccgcgaga gcgcaagcgc
     4381 cttcggggaa ctctgcgcgt atgtggagtc cctcggcgcc atcgtgcccg aggaaaccct
     4441 cctgaagggc ctcgcaggcg acgagaaggc gaagaactgg ttccggttcc tcctcgtggt
     4501 cggctcggag ttcttccgcg agcgcgagac cgacgatttc tatgcgcgct ggcacgtcga
     4561 tcaccggact gcagacgcgg tgcacaccgc gctcacgaag ctctactcct cccttgacga
     4621 cgaggaggtg ctgtccgagg ggcagatcat caaccgcttc ctcgaggaat tgaagggcgt
     4681 caactccgcc taccagcagg aggagatcct caagcgctgg ctctccatct cgaagaccat
     4741 ctcctcgaac ccgctttccg agtggggccg ggcgcatgcg ccggcgatcc gcacgaaggg
     4801 catccgcgat tatgcctacc tcgtgatcaa gcagaagggg gagcccatgc acttcgcgga
     4861 cgtcgcgaaa tccatcgtgg acatcttcgg caagaaggcg cacgtggcga ccacccacaa
     4921 cgagctcatc aaggactccc gcttcgtgct ggtgggccgc ggcctctacg cgctcgccga
     4981 gtgggggtac agcccgggcg tcgtgcgcga cgtgatccgc gacgtgctga agaagaagcc
     5041 gctgtcccgc gacgaggtca tcgccgaggt gaagaaggtc cggtacgtga aggacaacac
     5101 catcctcgtg aacttgaacg actcccggta cttcaagcgc cagaaggacg ggaagtacgc
     5161 cgccgtctaa tacttctcct cagcgaaccg cccggcatct gccgggcggt tcgcttcgct
     5221 tgatatactg agcggtatgt caggcggacc cggcagccct ctcccgttcc tcgataagat
     5281 cgagaagctg ttcggcttcg ggcacgagca gggattcgcg ttcaccgcgc ggcccttcat
     5341 catcgcggga ttggtcgcgg tgctgacctt cctcatcatc tcgcccgcgc agacgctgcg
     5401 gaacttcgag ttcctcgtct tcacctcgcc catctggctc tccgccgtcc tggtgcgctt
     5461 cgcgatctat cgcttcgtgg aggccaaccg gatgcagttc ctcgcccggc aggagtacgt
     5521 gctgctcgag atcaagctgc cgcgggacat caagaagtcg ccgctcgcca tggagacggt
     5581 gctcaccaac
//
LOCUS       DASPKG010000010        12157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_14255, whole genome shotgun sequence.
ACCESSION   DASPKG010000010 DASPKG010000000
VERSION     DASPKG010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12157)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12157
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14255"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..2308
                     /locus_tag="VEA36_00430"
     CDS             221..2308
                     /locus_tag="VEA36_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HYF28818.1"
                     /translation="MAKASDEKAKKSASHGGDYDASSITVLEGLDPVRKRPGMYIGTT
                     GPDGLHHLIWEIFDNSRDEAMGGFADDIEVALLPGGYVRVADNGRGIPVGIHPKTKVS
                     ALETIMTTLHAGGKFGGEGSGYKVSGGLHGVGASVVNALSEHTKVIVHKEGGVHVQEY
                     KIGKALAKVKQVGKTAAQGTIVMFKPDVTIFKDGIIWNWEKIVAHLRQQAYLVKGTRI
                     TVIDARETPTVDSEGLFYLREGGLDMPSMSFYFENGLRSLVGFYNKHQTVAHKNIFYV
                     DGSDGEVAVEVAFQYTDDISPRLLAFANNTYNAEHGTHVTGFKTALTRTLNSYARTAG
                     VLKESEENFTGDDVLEGITAVVSVKMPEIQFEGQTKAKLGSVEAQSAVATVFGSALAA
                     FLEENPDDAKAIANKSLLALKARKAAKAAKDSVLRKGALEGMTLPGKLADCQTKDASE
                     SELFIVEGDSAGGTAKTGRDRRTQAILPLRGKILNIERARLDKMLASEQIKNLVVALG
                     TAIGDIFDINKLRYHKIIIATDADVDGAHIRTLILTLFYRHFRPIIDGGFIYIAQPPL
                     YKIKKGKEILYAYSDAEKVRLLGEDAANAVEAMDESQGTEEEGDETPEEDVVTKKGSA
                     KVSIQRYKGLGEMNASELWETTMDPGNRVLKRVTVEDAEAADRIFDILMGTDVASRKS
                     FIQSNAKLANLDV"
     gene            complement(2354..3181)
                     /locus_tag="VEA36_00435"
     CDS             complement(2354..3181)
                     /locus_tag="VEA36_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HYF28819.1"
                     /translation="METHETTTTKTTSRSPAVTAFAVVGVIALILLGIGLAVYAVRYL
                     PEAMSGLGEAAVSLSRNFSGDDADVEVIDQPATPRDPGVVVTIPIGEETPAPGASPAP
                     AATPSAPAAPAYTAPAASAPAPFNPTFPQLSQTPTTRPVAQAPAAGFGSPDLTVEILA
                     VGYVTRDGASSTFREASRVPRGEQGAVRFRLSNRGTNESGRYEYEVRVRNEDNRTDTA
                     SGRAPSLGAGQSVISYAYFDARRSGDAEIDIEIDSGGDVRESNERNNTDSDDVEVRN"
     gene            3346..3999
                     /locus_tag="VEA36_00440"
     CDS             3346..3999
                     /locus_tag="VEA36_00440"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HYF28820.1"
                     /translation="MEPMTSRRGLAGAVSTAYTRKWTFLLAFLFVFTMSYALLAALGL
                     DPELRRPEVARVAPRLTATALDALPAEAPTRIVFPSLEREVRVVNPTSTNVAILDAAL
                     QSGAVRYPTSAKLGEEGNVVIFGHSSYLPVVHNGSYKAFNGIQDLMKGERIRVEGDGH
                     AYVYAVEKVYTANAGADAIPLTSTGRTLTLVTCDSFASKEDRFVVVATLVESYPLAN"
     gene            4129..5469
                     /locus_tag="VEA36_00445"
     CDS             4129..5469
                     /locus_tag="VEA36_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28821.1"
                     /translation="MDYSPPKPEDPDQRRVPWLLIIAAVLVLAVIGGISIWSPWDNGK
                     KTSRPSDKAAATSTMPPEKDKPAAAASVAAAPADPFAAPWSFDQFGNPLPQQQTLIVL
                     SAPAPTATAPSPAPAADPVPPAPRPPVRVTRTTTTATGPCTSQDSGREFRWRTIGARP
                     YAATRSEAFSDAKLDRMLYCTGVPQAHWAAIKQQLRTTAGKATWMPQGTAIGDMVFDR
                     RVGFLVPNVIVDVPGGVHGLVYEVEVEGVSYAFFLPFECWNWTRMRIRAPPRRSNCYD
                     IYYNYRTQSDIRKVNDAAVVSVHFPLTQAQVNALVQDPCTFVMDAEGNRFKPSLGRST
                     IICPPEYVSCWLPDAAARAAGLPLNRLPNGELHGAINFPCPKGRCKLSLPPARLDELI
                     RTVHCVGVHEYASVTPGLEGWRMYARFDIVRVGELQRTRSAGRLDRTLRGAKRS"
     gene            5548..7440
                     /locus_tag="VEA36_00450"
     CDS             5548..7440
                     /locus_tag="VEA36_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28822.1"
                     /translation="MKTTIISHLPNKQSIAIGLFAVLAMLATGFGIGLATGVVNLETA
                     EACCGVPPPAPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPVIPPPPPPPTVDECPNIP
                     GDQENIPDGLIRDGNGNCVQPPPPTDVCPNIPDVQTSVPPGLIKDAAGNCVQPPVDVC
                     PNIPDVQTSVPPGLIKDAAGNCVQPPQPPVDVCPNIPDVQTSVPPGLITDSNGNCVEP
                     PVNPPVDVCPNIPGNQATIPSGLIKDSAGNCVQPPANAPICVFLNVSDSSIDRGESVT
                     LSWKTQNATSISINQGIGEVTPVSEGSRSVSPNDDTTYVATVRNASASVNCEVRVDVD
                     EDRGGGGGGGRSKRTPRVELDNLPQPDALPFSFVYLDEIPYTGLELSPTGMVLYWLAL
                     IAVSAGLAYFIVFKAAPGMLRRITGVGGEVHGMLNRDTGHGGHEPQPVQHSTVAHPTG
                     PAAVAKAYSSYEGFRSFAQGGALTIDDIVTGLSRETHAPVEPAPIAPSSALPSQVEAA
                     PAPMAAAPAASFDAGQDVPGFIGALLSGNRDQTFDVVRQVTRRGDSAEDFLTHAIVAL
                     DDAYRAKVEGASVHPSIAALTASAAPAFLEKVIGALSSAVDGTYSTGVTGVKLAITRA
                     LAVVNG"
     gene            7602..7784
                     /locus_tag="VEA36_00455"
     CDS             7602..7784
                     /locus_tag="VEA36_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28823.1"
                     /translation="MNLREEWLQKGAAFLIVGVAAYVLVHAVGAIVELEPVERFLRYL
                     SRNIGHVGMPHFDRLY"
     gene            complement(7825..9570)
                     /gene="thrS"
                     /locus_tag="VEA36_00460"
     CDS             complement(7825..9570)
                     /gene="thrS"
                     /locus_tag="VEA36_00460"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HYF28824.1"
                     /translation="MASLEEKRHTLAHLLAAAVLERYPHAKAAIGPAIDTGFYYDFDF
                     SGGDAPGEAELAALQSAMEEILPSWTEMAGTEVSPMDARDRFKENPYKLEIIEGIVEL
                     GEPITLYTAGTFTDLCRGGHAEHPARDLAPDTFALDRIAGAYWRGDEKNPMLTRIYGL
                     AFDAKEELAAYQAQREAAKERDHKKLGKELDLFLFSELVGPGLPLWTPRGTLVRHLLD
                     DFVWQLRRAQGYEKVEIPHITKKELYETSGHWDKFKDELFRITTREGHEFAMKPMNCP
                     HHTQIYARKQWSYRELPQRYANTTTCYRDEQSGELSGLSRVRAFAQDDAHVFCRMQDA
                     KAEFLKVWDIVHAFYPAFGFELRVRLSTHDPAHPEQYLGDAERWEEAEGMLREIVAEK
                     GIDALPGVGEAAFYGPKLDFIAQDSLGRSWQVATIQLDMNQPERFDLFCINEKGEKER
                     IVMIHAAIMGSLDRFLSILIEHTAGNFPVWLAPIQARVLPISGDKHGAYAQEIMGRLK
                     AAGVRADLDEGNESLGKRIRSAKQDKIPYLLVVGDAEAAAGTVAVESRDAGKQDAMAL
                     EAFLARIAEEVQERK"
     gene            complement(10001..>12157)
                     /gene="dnaE"
                     /locus_tag="VEA36_00465"
     CDS             complement(10001..>12157)
                     /gene="dnaE"
                     /locus_tag="VEA36_00465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HYF28825.1"
                     /translation="LAVSDLLTYAKGNGILATTRGSAAGSLVSYLTGITNVDPLLYKL
                     PFERFLNPERPKAPDIDMDFADNRRDDMIAYAKAKYGEDKVAQIGTFGTLMARAAVRD
                     VSRALGHAYRTGDRIAKLIPLGSQGFPMTIDRALELEPELKELYEQDEDTREILDLAR
                     QIEGCVRHTGVHAAGVVIAPTPLTDWTPLQIDPKTGKLITQYDMHAIEDAGLLKFDFL
                     GIKNLAILSDAVERVQETRGIFIDIEQVPIDDQKTFAMLARGETEGTFQLNGAGMTRY
                     LKELQPTTIHDINAMVALYRPGPMETIPQYIERKKNPRLIHYLDERMKEYLDFSYGLL
                     VYQEDVLLTAIKLGGYSWLEADTLRKAMGKKIPAEMEAQKEKLIRGFMEFGKLSKQKA
                     ETIWKLIEPFAAYGFGKAHAACYGKVAYQTAYMKANYPVEYMTALLTADAGDTEQIAI
                     LAAECERLGIPLLPPDINESGTDFTVAGDAKDTIRFGLMSIKNFGQGISEAIIEERRT
                     RGPFKDLADFLARVGSRNLNRKSLECLIKSGALDRFGERGHLSANIELLLTFHRDATM
                     VATQDSLFGASFGTPTLLLPKPTGEMPLAEKLAWEKELLGIYVSGHPLDAHAGVASKA
                     TITIGQILEEPQAGRPVILPVLVQEVRTILTKSGEKMAFIKFEDKSASIEAVAFPKLY
                     KEHGHLLATGQCLLVKGSVSGRNGETSLALDNLKAL"
BASE COUNT         2107 a         3972 c         3914 g         2164 t
ORIGIN      
        1 ccgacgtccg gccgcacgcc gttgaggtag tccagaagct gcgcgcccgt cgcgccgccc
       61 caaagattaa agctcatgat gcgcatggca cccctccccg tgttttcagg aactatggtt
      121 gacgcctcgc gccatacaac cactgccaga atgaattgca aatgaatggc tgagctcggg
      181 ctctcctggc cgatttgtgg tatactggaa tcacgaaaat atggctaaag ctagcgacga
      241 aaaggcaaaa aagagcgcct ctcacggagg cgattacgat gcctcctcca tcacggtcct
      301 ggagggcctc gacccggtcc gcaagcgccc cggtatgtac atcgggacca ccgggcccga
      361 cggcctccac cacctcatct gggagatctt cgacaactcg cgcgacgagg ccatgggcgg
      421 gttcgcggac gatatcgaag tggcgctcct gccgggcggg tacgtccggg tcgcggacaa
      481 tggccgcggc atcccggtgg gcatccaccc gaagacgaag gtctcggcgc tcgagaccat
      541 catgacgaca ctgcatgccg gcggcaagtt cggcggggaa gggtccgggt acaaagtgtc
      601 aggcggcctc cacggcgtgg gcgcctcggt ggtgaacgcg ctctcggagc acacgaaggt
      661 gatcgtacac aaggagggcg gggtgcacgt gcaggagtac aagatcggga aagccttggc
      721 gaaggtgaag caggtcggga agaccgccgc gcagggcacc atcgtcatgt tcaaacccga
      781 cgtcaccatc ttcaaggacg gcattatctg gaactgggaa aagatcgtcg cgcacctgcg
      841 ccagcaggcg tacctcgtga agggcacccg catcacggtc atcgacgcgc gcgagacccc
      901 gacggtggat tctgaggggc tcttctatct gcgcgagggc ggcctcgata tgccctccat
      961 gtccttctac ttcgagaacg ggctccgctc cctcgtcggg ttctataaca agcaccagac
     1021 ggtggcccac aagaacatct tctacgtcga cgggagcgac ggcgaggtcg cggtcgaggt
     1081 cgcattccaa tacaccgacg atatctcgcc gcgcctcctt gcgttcgcga acaataccta
     1141 caatgccgag cacggcaccc acgtgacggg cttcaagacc gcgctgacgc gcaccctgaa
     1201 ttcgtacgcg cgcacggccg gtgtcctcaa ggagagtgag gagaacttca ccggcgacga
     1261 cgtgctcgag ggcattacgg cggtcgtctc ggtcaagatg cccgagatcc agttcgaggg
     1321 gcagaccaag gcgaagctcg gctccgtcga ggcccagagc gcggtcgcga ccgtcttcgg
     1381 ttcggccctc gccgccttcc tcgaggagaa tccggacgac gcgaaggcga tcgcgaataa
     1441 gtcgctactt gccttgaagg cccgcaaggc cgcgaaggcc gcgaaggact ccgtgctgcg
     1501 caagggcgca ctcgagggca tgacgctccc ggggaagctt gcggactgcc agaccaagga
     1561 tgcgtccgaa tcagagctct tcatcgtgga gggagactct gcgggcggga ccgccaagac
     1621 cggccgcgat cgccgcacgc aggccatctt gccgcttcgc gggaagatcc tcaacatcga
     1681 gcgggcgcgc ctcgataaga tgctcgcctc cgagcagatc aagaacctcg tggtcgcgct
     1741 cggcaccgcg atcggggaca tcttcgatat caataagctc cggtaccaca agatcatcat
     1801 cgcgaccgat gccgacgtgg acggcgcgca catccgtacg ctcatcctca ccctcttcta
     1861 ccgccacttc cgccccatca tcgacggcgg gttcatctac atcgcccagc cgcctctgta
     1921 caagatcaag aaggggaagg agatcctcta cgcatactcg gatgcggaga aggtgcgcct
     1981 gcttggagaa gacgctgcga acgcggtcga ggccatggac gagagtcagg ggaccgaaga
     2041 ggagggggac gagacgcccg aggaggacgt cgtaacgaag aagggaagcg cgaaggtctc
     2101 catccagcgg tacaagggtc tcggcgagat gaacgcctcc gagctctggg agaccaccat
     2161 ggacccgggc aatcgagtgc tcaagcgggt cacggtcgag gacgctgagg cggccgaccg
     2221 catcttcgac atcctcatgg gcaccgacgt cgcgagccgg aagtccttca tccaatcgaa
     2281 cgcgaagctc gcgaacctgg acgtataaat aagaaaaggc ccgcagcttt cgccgcgggc
     2341 cttttcttat agattagtta cgcacctcga cgtcgtcgga gtccgtatta ttgcgctcgt
     2401 tcgattcgcg cacgtcgccc ccggaatcga tctcgatgtc gatctccgca tcgcccgagc
     2461 ggcgagcgtc gaagtacgca taggagatga ccgactgccc agcgccaagc gagggcgcac
     2521 gtccgcttgc ggtatccgtg cggttgtcct cgttgcggac ccggacctcg tattcatagc
     2581 ggccgctctc gttcgtgccg cgattggaaa ggcggaagcg gaccgcgccc tgctcgccgc
     2641 gcgggacgcg ggatgcttcg cggaaagtcg aggaggcgcc gtcgcgggtc acgtagccca
     2701 ccgcgaggat ctccacggtg aggtcagggg aaccgaagcc agcggctggg gcctgcgcga
     2761 ccggacgcgt cgtcggggtc tgggagagct gcgggaaggt cgggttgaag ggcgcaggtg
     2821 cggaggccgc aggcgcggtg tacgccggtg cggccggagc cgatggcgtg gctgccgggg
     2881 caggggaggc gccgggcgcc ggggtctcct cgccgatcgg gatcgtcacg acgacgcccg
     2941 ggtcgcgcgg ggtcgcgggc tggtcgatga cctccacgtc cgcatcgtcg ccggagaagt
     3001 tccgggacag ggacaccgcg gcctcgccaa ggccggacat cgcctccgga aggtagcgca
     3061 cggcgtagac ggcgagcccg atcccgagca ggatgagcgc gatcacgcca accacggcga
     3121 acgccgtcac cgccggcgag cgggacgtcg ttttggtcgt ggtggtctca tgggtttcca
     3181 taagggtaga ttatgcttct tgcaagatat ttgtcaatat ttcccgccca tacccttcag
     3241 catacgttca ccaaactgaa ggtaccttga agccgggaaa tgtctagaaa tggccggaaa
     3301 cttgacaata aaagggattt ggggtaatca tgcgcaagac cagatatgga accgatgacc
     3361 tcaaggcgcg gccttgccgg ggctgtaagc acggcgtata ccaggaagtg gaccttcctg
     3421 ttggccttcc tcttcgtctt cacgatgtcc tacgccctcc ttgcggcgct tggcctcgat
     3481 cccgagctcc ggaggccgga ggtggcgcgc gtggccccgc ggctcaccgc caccgcgctc
     3541 gatgcgctcc cggccgaggc cccgacgagg atcgtgttcc cgtcccttga acgcgaggtc
     3601 cgggtcgtca acccgacctc caccaatgtg gcgatcttgg acgccgcgct ccagtccgga
     3661 gcggtccggt atcccacctc cgccaagctc ggcgaggaag gaaacgtcgt catcttcggc
     3721 cattcgagct atctgcccgt cgtgcataac gggtcctaca aggccttcaa tggcatccag
     3781 gacctcatga agggcgagcg catccgggtt gagggcgacg gccacgccta tgtgtacgcg
     3841 gtcgagaagg tgtataccgc gaacgcggga gcggatgcga tcccgctcac gtccaccggc
     3901 cgcacgctga ccttggtcac ctgcgactcc ttcgcgtcga aggaggaccg gttcgtcgtg
     3961 gtcgcgaccc ttgttgagag ttatccgctc gcgaactagt tcgcggacgg ataactctct
     4021 gcaaagagag tagaaaccag agtacctgta ccttgttaat tgcttggttc taaccgccct
     4081 gcggggcaca gatgaaaggg catcccgatg agggacgatc cacctccggt ggactactca
     4141 ccaccgaagc cggaagaccc cgaccagcgt cgggtgccat ggcttctgat cattgccgcc
     4201 gtcctcgtgc tcgcggtgat cggcggcatc tcgatctggt cgccgtggga caacggcaag
     4261 aagacatcac ggccgtccga caaggcagcc gcgacatcaa ctatgccacc cgagaaggac
     4321 aagccagctg ctgcggcatc tgtcgccgcg gcgcctgcgg atccgttcgc tgctccctgg
     4381 tccttcgacc agttcgggaa cccgctgccg cagcagcaga cgctcatcgt cctgtctgcg
     4441 ccggctccga cggcaacggc tccatcgcca gcgcccgctg ctgatcccgt tccgccggcc
     4501 ccacggccgc cggttcgggt tacacggacc acgaccactg ccaccggccc ctgcacgtcg
     4561 caagacagcg gcagggaatt ccggtggcgg acgatcggtg cgcggcccta cgccgcgacc
     4621 cgctcggagg cgttctcgga cgccaagctc gatcgcatgc tctactgcac cggcgttccg
     4681 caggcgcact gggctgcgat caagcagcag ctccgcacga ccgccggcaa ggcgacctgg
     4741 atgccgcaag gcaccgcgat cggcgacatg gtgttcgacc gccgcgtcgg attcctagtg
     4801 cctaacgtca tcgtggatgt gcccggcggc gtgcacgggc tcgtctacga ggtcgaggtc
     4861 gagggcgtct cgtacgcctt cttcctgccc ttcgagtgct ggaactggac ccgcatgcgg
     4921 atccgggcac cgcctcgcag gtccaactgc tacgacatct actacaacta ccgtacgcag
     4981 tcggacatcc ggaaggtgaa cgacgccgcc gtcgtgagcg tgcatttccc gctcacccag
     5041 gcgcaggtca acgcgctcgt gcaggacccc tgcacgttcg tgatggacgc ggaagggaac
     5101 cggttcaagc cgagcctcgg ccgctccacc atcatctgcc cgcctgagta cgtctcctgc
     5161 tggttgccgg acgctgctgc acgtgcagcc ggcctcccgc ttaaccggct gccgaacggc
     5221 gaactgcacg gggcgatcaa cttcccgtgc ccgaaggggc gctgcaagct ctcgctgccg
     5281 ccggcgaggc tcgacgagct catccggacc gtgcactgcg tcggcgtcca cgagtacgcg
     5341 tcggtgacgc ccggcctgga aggatggcgg atgtacgccc gcttcgacat cgtccgggtc
     5401 ggcgaactgc agaggacgcg atcggccgga aggctcgacc gcacgctccg cggagccaag
     5461 cgttcctagc ccctcgtggg cttacctcgg gcaggtgggg gtcctccggg atccctgcct
     5521 cgccctctta tacaacaatt accaaccatg aagaccacca tcatttcaca tctgccgaac
     5581 aaacagtcga tcgcgatcgg cctgttcgct gtgcttgcca tgctcgcgac tgggttcggc
     5641 atcggtcttg caaccggggt tgtgaacctc gagacggccg aggcctgctg cggcgttccg
     5701 cctccggcac cacccccgcc tcctcctcca ccccctccgc cgccaccacc cccgcctcct
     5761 ccacctcctc cacctccgcc gccgccaccc ccgccgccgg tgatcccgcc cccgcctccg
     5821 ccgcccacgg tggacgagtg tccgaacatc ccgggcgacc aggagaatat cccggatgga
     5881 ctcatccgcg acggcaatgg caactgcgtg cagccgcccc cgccgactga cgtctgcccg
     5941 aatatccctg acgtgcagac ctcggtcccg cctgggctta ttaaggatgc tgctggcaac
     6001 tgcgtgcagc cgccggtcga cgtatgtccc aatatcccgg atgtccagac gtccgttccg
     6061 cccgggctca tcaaggacgc cgcagggaat tgcgtgcagc cgccccagcc gcctgttgac
     6121 gtgtgtccga acattcccga tgtgcagact tcggtgccgc ctggcctcat cacggacagc
     6181 aacggtaact gtgttgaacc tccggtcaat ccgccggtcg acgtctgtcc gaacatcccg
     6241 ggtaaccagg caaccatccc gtccggcctc atcaaggact cggcaggcaa ctgcgtgcag
     6301 cctccggcga acgcgcctat ttgcgtgttc ctcaacgtga gcgacagctc gatcgatcgc
     6361 ggcgagtcgg tgacgctttc ctggaagacc cagaacgcca cctcgatctc catcaatcag
     6421 ggtatcgggg aggtgacccc ggtatcggag ggttcccgct cggtgagccc gaacgacgac
     6481 accacgtacg tcgcgacggt gcgcaacgca tccgcatccg tgaactgcga ggtccgcgtg
     6541 gacgtggacg aggaccgtgg cggtggcggg ggtggcggac gctcgaagcg cactccgcgc
     6601 gtcgagctcg acaacctccc gcagcccgac gcgctcccgt tctccttcgt gtacctcgat
     6661 gagatcccgt acacgggcct cgagctctcg ccgaccggca tggtcctcta ctggctcgcg
     6721 ctcatcgcgg tctcggcagg cctcgcatac ttcatcgtct tcaaggcggc gccgggcatg
     6781 ctccgccgca tcacgggcgt cggcggtgaa gtgcacggca tgctgaaccg ggatacgggc
     6841 cacggcggcc acgagcccca gcctgtccag cactcgacgg tcgcgcaccc gacggggccc
     6901 gctgcggtcg cgaaggcgta ctcctcgtac gagggcttcc gttccttcgc ccagggcggc
     6961 gcgctcacca tcgacgacat cgtcacgggg ctttcccgcg agacccatgc gccggtcgag
     7021 cctgccccga tcgcgccgtc ctctgcgctc ccgtcgcagg tcgaggcagc acccgcacct
     7081 atggccgctg cgcctgccgc atccttcgat gccggccagg acgtgccggg cttcatcggg
     7141 gcgctcctct cgggcaaccg cgaccagacc ttcgacgtcg tgcgccaggt gacccgccgc
     7201 ggcgacagcg ccgaggactt cctcacgcac gcgatcgtcg cgctcgatga cgcgtaccgc
     7261 gcgaaggtgg agggcgcatc cgtgcatccc tcgatcgcgg ccctgaccgc ctcggccgcc
     7321 ccggccttcc tcgagaaggt gatcggcgcg ctctcgtcgg cggtcgacgg cacctactcg
     7381 accggggtca cgggcgtgaa gctcgcgatc acccgggcgc tcgcggtcgt caacggctaa
     7441 gcgcctccgt aaagaaacgc cgccccctcg ggagcggctt ttctttttac caaaggggcg
     7501 tatgctcccc gcaggaggat cggttcaagt tcacgcatga cgacgatcct gatcgcgctc
     7561 aatcacgctt cttagacacg ggagaggaga ccatgagaga aatgaacctg cgagaggaat
     7621 ggctccagaa gggagcagcg ttcctcatcg tgggtgtagc tgcgtatgtc ttggtgcatg
     7681 cggtgggcgc gatcgtggag cttgaaccgg tggaacggtt cctgcgctac ctgtcgcgga
     7741 acatcggcca cgttgggatg ccgcatttcg accgcctcta ctgaacaccc gcgaacggtg
     7801 acgccggccc caagccggcg ttcgctattt ccgctcttgt acctcctcgg cgatgcgcgc
     7861 aaggaacgcc tcgagcgcca tggcgtcctg cttgcccgca tcccggctct ccaccgcgac
     7921 cgtgcccgct gccgcctctg cgtctcccac cacgagcagg tacgggatct tgtcctgctt
     7981 cgcgctccgg atgcgcttgc cgagcgattc gttcccttcg tccaaatccg cgcgcacccc
     8041 tgcggccttc aagcggccca tgatctcctg cgcataggcg ccgtgcttgt ctcctgagat
     8101 gggcagcacg cgggcctgta tgggggcgag ccagacgggg aagttccccg ccgtgtgctc
     8161 gatgagtatg gaaaggaagc ggtccaagga gcccatgatg gccgcatgga tcatcacgat
     8221 gcgctccttc tcgcccttct cgttgatgca gaagaggtcg aagcgctccg gctggttcat
     8281 atcgagctgg atggtcgcga cctgccagga gcggccgagc gaatcctgcg cgatgaagtc
     8341 gagcttcggg ccgtagaagg cggcctcgcc gacgcccggc agcgcatcga tgcctttctc
     8401 cgcaacgatc tcccgcagca tcccttcggc ctcctcccag cgctcggcat ccccaaggta
     8461 ctgctcgggg tgcgcagggt catgggtgga gaggcggacc cgaagttcga atccgaaggc
     8521 cggatagaac gcgtggacga tgtcccacac cttaaggaac tccgccttcg cgtcctgcat
     8581 ccggcagaag acgtgcgcat cgtcctgcgc gaacgcgcgc acccgggaga gccccgacaa
     8641 ttccccggac tgctcgtcgc ggtagcaggt ggtggtgttc gcgtagcgct gggggagctc
     8701 gcggtagctc cactgctttc gtgcgtagat ctgcgtatgg tgcgggcagt tcatcggctt
     8761 catcgcgaac tcgtgaccct cgcgcgtcgt gatacggaag agttcatcct tgaacttgtc
     8821 ccagtgcccg gaggtctcat aaagctcctt cttcgtgata tgcgggatct ccaccttctc
     8881 gtatccctgc gctcttcgca gctgccagac gaaatcatcg aggagatggc gcacgagggt
     8941 gccgcgcggg gtccacaagg gaagcccggg gccaacgagc tcggagaaga ggaagaggtc
     9001 gagctccttc ccgagcttct tatggtcgcg ctctttcgcg gcctcgcgct gcgcctggta
     9061 tgccgcgagt tcttccttgg catcgaaggc aaggccatag atgcgggtga gcatggggtt
     9121 cttctcatcg ccgcgccagt aggcgccggc gatgcggtcg agcgcgaagg tgtcgggcgc
     9181 aaggtcgcgc gcggggtgct cggcatgccc gccccggcag aggtcggtga aggtaccggc
     9241 cgtgtacagg gtgatgggct cgccgagctc cacgatcccc tcgatgatct cgagcttgta
     9301 cgggttctcc ttgaagcggt ctcgcgcgtc cataggagac acttcggtgc cggccatctc
     9361 cgtccaggag gggaggatct cctccatcgc gctctggagc gcggcgagct ccgcctcgcc
     9421 cggagcgtct ccgccggaga aatcgaagtc gtagtagaag cccgtatcga tcgcgggccc
     9481 gatcgccgcc ttcgcatgcg ggtaccgctc gaggacggcg gcggccagga ggtgggcgag
     9541 cgtgtggcgt ttttcctcga gcgaggccat gtaggaatga ggatagcacg gccgtttcta
     9601 acctaaaaag caagccggac gaccccatga gggccgcccg tgctgagctg gatgcgctag
     9661 tagtcgcgcg gcttgacggc ggtccttgga cgagccctga accagacctt catgccgcga
     9721 cgccgcagcc acttcttgat cttgcgccgt agtctctgct tacgcgattg cgccatcatg
     9781 tcctcctagt cgccgatgtg gccgccgtcc ttgtggtcga gcggcagggt gagtcgccgg
     9841 tgcggtcccg ggagccggac atcgcctgga tgcacgggcg cagcgcgggt gcgcggaagg
     9901 gatgccagcc gttctggcag accggaatgc ggtcgttggg gtgcgatgct cggtatgttc
     9961 gccagcctcc ttgtgcaggt tcagtcctac ctcttctata tcataatgcc ttgaggttat
    10021 cgagcgcgag cgaggtctcg ccgttgcggc ctgagaccga tcccttcacg aggaggcact
    10081 ggccggtggc aagcaggtgc ccatgctcct tgtacagctt cgggaaggcg accgcctcga
    10141 tggacgcgct cttgtcctcg aacttgatga acgccatctt ctcccccgac ttcgtgagga
    10201 tggtgcggac ctcttgcacg agcacgggga ggatcaccgg acgcccggcc tggggctcct
    10261 cgaggatctg cccgatggtg atggtcgcct tggacgcaac gcccgcatgc gcatcgagcg
    10321 ggtgcccgga gacgtagatg ccaagaagct ccttttccca ggcgagcttc tccgcgagcg
    10381 gcatctcgcc ggttggcttc gggaggagaa gggtgggcgt accgaacgat gcgccgaaga
    10441 gggagtcctg tgtagcgacc atggtcgcgt cccgatggaa ggtgagcaag agctcgatgt
    10501 tcgcggacag atgcccgcgc tcgccgaagc gatcgagcgc gcccgacttg atgaggcact
    10561 ccagtgattt tctattcaaa ttcctcgagc caacccgcgc gaggaagtcc gcgaggtcct
    10621 tgaagggccc acgcgttcgc cgttcctcga tgatcgcttc ggagatgccc tgcccgaagt
    10681 tcttgatgga catgaggccg aagcggatgg tgtccttggc gtcgcccgcg accgtgaagt
    10741 ccgtaccgct ctcattgata tcaggcggga gaagcgggat gccgagccgc tcgcactcgg
    10801 ccgcgaggat ggcgatctgc tcggtatcgc cggcgtccgc ggtgagaagc gcggtcatgt
    10861 actcgacggg gtagttcgcc ttcatgtacg cggtctggta cgccaccttg ccgtagcagg
    10921 cggcgtgcgc cttaccgaag ccgtaggcgg cgaagggctc gatcagtttc cagatcgtct
    10981 ccgccttttg cttggaaagc ttcccgaatt ccatgaagcc gcggatcagc ttctccttct
    11041 gcgcctccat ctccgccggg atcttcttgc ccatggcctt gcggagcgta tcggcctcga
    11101 gccaggagta ccccccgagc ttgatcgcgg tgaggagcac gtcctcttgg tacacgagga
    11161 ggccgtacga gaaatccaga tactccttca tgcgctcgtc caggtagtgg atgagccgcg
    11221 ggttcttctt gcgctcgatg tactgcggga tggtctccat ggggccgggg cggtagagcg
    11281 ccaccatggc gttgatgtcg tggatggtgg tgggctggag ctctttcaga taccgcgtca
    11341 tgccggcgcc gttcaactgg aaggtgccct cggtctcgcc gcgcgcgagc atagcgaagg
    11401 tcttctggtc gtcgatgggt acctgctcga tgtcgatgaa gatgccgcgg gtctcctgca
    11461 cgcgctcgac cgcgtccgag aggatggcga ggttcttgat accgaggaag tcgaacttca
    11521 ggaggccggc gtcctcgatg gcgtgcatat cgtactgggt gatcaatttc ccggtcttgg
    11581 gatcgatctg gagcggggtc cagtcggtca gcggcgtcgg cgcgatcacc acacccgcgg
    11641 cgtgcacgcc ggtatgccgc acgcacccct cgatctgccg cgcgaggtcg aggatctcgc
    11701 gggtgtcctc gtcctgctcg tagagctcct tcagttccgg ctcgagctca agcgcgcggt
    11761 cgatggtcat ggggaagccc tgggagccca gtgggatgag cttcgcgatg cggtcgccgg
    11821 tccggtacgc atggccgagc gcccgcgaca cgtcgcgcac tgcggcgcgg gccatcaggg
    11881 tcccgaacgt gccgatctgc gcgaccttgt cctcgccgta cttggccttg gcgtacgcga
    11941 tcatgtcgtc ccggcggttg tcggcgaagt ccatgtcgat gtcgggcgcc ttcgggcgct
    12001 cggggttgag gaagcgctcg aagggaagct tatagagcag cggatccacg ttcgtgatgc
    12061 cggtgaggta cgagacgagg gagccagctg cggagccgcg cgtcgtcgcg aggatgccgt
    12121 tgccttttgc gtaggtgagg aggtcggaca ccgcgag
//
LOCUS       DASPKG010000011         7096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_420649, whole genome shotgun sequence.
ACCESSION   DASPKG010000011 DASPKG010000000
VERSION     DASPKG010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420649"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1921)
                     /locus_tag="VEA36_00470"
     CDS             complement(<1..1921)
                     /locus_tag="VEA36_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYF28826.1"
                     /translation="MLKRLEIAGFKSFARKTVLDFASATTAIVGPNGSGKSNVAEAFR
                     FALGEQSMKSMRGKRSEDLIWAGSHTNPRANRAAVAIVFDNTKRIFPKLDFEEVTVER
                     AVFRDGASEYSINGSKVRLRDVQELLAGANIGDTGHHIISQGEADRILLAHPRERRSM
                     LEDALGLKVFEFKKADAERKLQKTEENVREVESLRREIAPHIRFLEKQVEKLERAEAF
                     ARELAELARTYFAIEEAYIDAEKKAAEARKRSAEERLATASAELAAFDERATTDSEGM
                     KRSEAIRQTEKEVDRLTQSRGELARELGRVEGGLHEAKKRSAQVAREPYVKVPREDLA
                     ALRAEIEKQTAGEDATPDALRAALTTVRNMITAFFDRFAAPADDYLAEEEQTVMRLEA
                     ERESLLEKDRTLTADIDKARSSLMKAREAALAHEETGREEKRRILGLAESKAHEEGQM
                     ERESGRLRELQYLADELERDRAEILALAGSSAFSYEPLTALPAEERRAQEDRKRQLER
                     LKIRLEEAGGTGDDVRKEFEDATAREAFLARELEDLAKSAEGLRALITDLDTELQKTF
                     AEGLAKVNASFGEFFSLMFGGGGARLVLEQEPERDTDEDEEEYEEDRTKQWKPGIEIA
                     VTLPRKKVQSLMQLSG"
     gene            2013..2636
                     /locus_tag="VEA36_00475"
     CDS             2013..2636
                     /locus_tag="VEA36_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HYF28827.1"
                     /translation="MADGTTAAALQRPSARTLAEAHELLTRMTRINADIRAIEEEAAI
                     KTAADRRVISEAEERIAPVLAEAKLKLDPLKKEAQEVGEGFRRFTERHPKLLPPPADR
                     LKTLTFNTGHTYLRQEIGLKVVTKGKNDDAAIEEIKAKGWEADFIEYKPFLRRNELKR
                     TEHRQRVEELTCLTLRMTRKITIGLPNGIQFIGKDDDRGKFSWEFES"
     gene            complement(2761..3036)
                     /locus_tag="VEA36_00480"
     CDS             complement(2761..3036)
                     /locus_tag="VEA36_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28828.1"
                     /translation="MRFLRSFASLTFVGVAVPNWVSIYGAEHWTGDLQRVAAIVFVLT
                     TVGGFIVAVGLAFYALYRQHPGHFGRAAWLILCSCLGVGIRALIDLV"
     gene            complement(3078..3764)
                     /gene="rnc"
                     /locus_tag="VEA36_00485"
     CDS             complement(3078..3764)
                     /gene="rnc"
                     /locus_tag="VEA36_00485"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HYF28829.1"
                     /translation="MTEFKDYADKLELSFQNPDLFVEALTHRSYLNENRGAGNHNERL
                     EFLGDAVLELAVTQFLFTRFPNKPEGDLTAYRAALVNTYSLADTAKEIGLNDMLLLSK
                     GEKKDTGRARDIILANAFEALLGAIYLDQGYEAAEQFVAKHLYPKIDRVIATRAYQDA
                     KSQFQESAQEKRSVTPTYQTLSEEGPDHDKQFTVGVYLADEEIARGEGKSKQEAEQAA
                     AQAALDKTGW"
     gene            complement(3777..4682)
                     /gene="nusB"
                     /locus_tag="VEA36_00490"
     CDS             complement(3777..4682)
                     /gene="nusB"
                     /locus_tag="VEA36_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HYF28830.1"
                     /translation="MANRHLARSVVLQTLFEWDTTHADQSAASEMLARNVEEFGGDDT
                     DRPFMEALLAGVLAKQEELDLIITKAAPDWPLDRIAPVDRNILRIGLYELLFADRAQV
                     PAKVAINEAIELAKVFGGDSSGRFVNGVLGAVYKELGEPGKDETGKKDKVKREALPLE
                     KLGGAIIYAYHDGQYYLALVHDVFGRWTLSKGHIENDPSPEEGVRRVVKEELGLENVT
                     VEEQVGENEYVASHPEKGKIRKHVWFFLVKSEFEPVELKKTGGLDDAKWFRLPDILDL
                     NFYDDMLPIVTAAVQKLLDRSGKGA"
     gene            complement(4782..5144)
                     /gene="rpmF"
                     /locus_tag="VEA36_00495"
     CDS             complement(4782..5144)
                     /gene="rpmF"
                     /locus_tag="VEA36_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HYF28831.1"
                     /translation="MSVRMRHTRSHTRNRRSHHALAAGAVVTDKESGAHRLPHRLDES
                     TGMYRGKLIVPERKQEVKVEKKRREKNEAEQGDLGAREPVHEEKHTVADGIEKKQSLI
                     GRMTKGKAKSRSGFGGGA"
     gene            complement(5536..6117)
                     /locus_tag="VEA36_00500"
     CDS             complement(5536..6117)
                     /locus_tag="VEA36_00500"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HYF28832.1"
                     /translation="MKFILGVDEAGRGPLAGPVVVGVIAVADGYDLLAAFPGLNDSKK
                     LSEKKREALFQLLQKEMHAGNVRASVQLVGATYIDQGGIAPAIRHAVARGIEKLLPNP
                     HEGKVFLDGSLKAPAEYAQETVVGGDGIVPAIMLASIAAKVTRDKHMEMLAKEYPAYG
                     FEKHKGYGTKAHIEAIRTFGPCIEHRRSFLTRI"
     gene            6159..>7096
                     /locus_tag="VEA36_00505"
     CDS             6159..>7096
                     /locus_tag="VEA36_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HYF28833.1"
                     /translation="MAGRYIHLHTHSHYSFLQALPKVPELVKAAKAAGMDALGLTDAG
                     NLHAGIEFYKAAEKAGIKPILGVDAYLAQGSRRQVHEVPAERGGRTRLVLLAMNNEGY
                     QNLLTLITASFTEGYHERPHMDKELLRSHHAGLIALIPSFAGEVAQHLKQGDKKGAAA
                     AIEEYVSIFGKENVYLEITHHPKVEGHAKRMEEIRKLAKESGVPLVAQHDVYYLKPSD
                     REATEVMRRIAHGERGRNEDEDFSFVSESQMREWFADVPEAVDASGEIADRCSIHFEL
                     GSWNFPAVEVEPGKTHEEMLRERTYDGLAMRGMDKT"
BASE COUNT         1223 a         2340 c         2213 g         1320 t
ORIGIN      
        1 cgcccgagag ctgcatcagc gactgcacct tcttccgggg cagggtcacc gcgatctcga
       61 tgcccggctt ccactgcttc gtacggtcct cctcgtattc ctcctcgtcc tcgtccgtat
      121 cccgctcggg ttcctgctcg agcacgaggc gcgcgccgcc gccgccgaac atgagcgaga
      181 agaattcccc gaaagaggcg ttcaccttcg cgagcccctc ggcgaaggtc ttctggagct
      241 cggtatccag gtccgtaatg agggcgcgca gtccctcagc ggatttcgcg agatcctcga
      301 gctcgcgggc aagaaacgcc tcgcgcgcgg tggcgtcctc aaattccttc cgcacgtcgt
      361 cgccggtgcc gcccgcttct tccagccgga tcttgagccg ctcgagctgg cgcttgcggt
      421 cctcctgcgc gcggcgctcc tcggccggca gcgcggtgag gggttcgtat gaaaatgcgc
      481 tgctgccggc gagcgcgagg atctcggcgc gatcgcgctc gagctcgtcg gcgaggtact
      541 ggagctcgcg cagccgcccg ctctcccgtt ccatctggcc ctcctcatgc gccttgctct
      601 cggcaagccc caggatccgg cgcttctcct cccgccccgt ctcctcatgc gcaagcgcgg
      661 cctcgcgggc cttcatgagc gagctccggg ccttatcgat atctgcagtt agggtgcggt
      721 ccttctcgag gaggctctcg cgctccgcct cgaggcgcat cacggtctgc tcctcttccg
      781 cgaggtaatc gtcggcgggc gcggcgaagc ggtcgaagaa ggccgtgatc atattccgaa
      841 ccgttgtaag ggcagcgcgg agcgcgtcgg gcgtcgcatc ctcccctgcc gtctgcttct
      901 cgatctcagc gcgcagcgca gcgaggtcct cccgcggcac cttcacgtac ggctcgcgcg
      961 cgacctgggc gctgcgtttc ttcgcctcgt ggagcccgcc ctcgacccgt ccgagctcgc
     1021 gggcgagctc gcctcggctc tgggtgaggc ggtccacctc cttctccgtc tggcggatgg
     1081 cctcggagcg cttcataccc tcggagtcgg tggtggcgcg ctcatcgaag gctgcgagct
     1141 cagcgcttgc cgtggcgagc cgctcctcgg cgctgcgctt gcgcgcctcc gccgccttct
     1201 tctcggcatc tatgtacgct tcctcgatcg cgaaataggt gcgggcgagc tcggcgagct
     1261 cgcgggcgaa ggcctccgcg cgctccaatt tctccacctg cttctctagg aaccggatgt
     1321 gcggcgcgat ctcccgccgc aaagactcca cctcccgcac gttctcctcg gtcttctgga
     1381 gcttgcgttc cgcgtccgcc ttcttgaact cgaagacctt gagcccgagc gcgtcctcga
     1441 gcatggagcg gcgctcgcgc ggatgcgcca agaggatgcg gtccgcctcc ccctggctga
     1501 tgatgtggtg ccccgtatcc ccgatgttcg cgccggcgag cagctcctgc acgtcccgga
     1561 gccggacctt cgaaccgttg atggagtact cgctcgcgcc gtcccggaag accgcgcgct
     1621 cgaccgtgac ctcctcgaag tcgagcttcg ggaagatgcg cttcgtgtta tcgaagacga
     1681 tcgcgaccgc ggcgcggttc gcccgtggat tggtgtgcga cccggcccag atgaggtcct
     1741 cgctgcgctt cccgcgcatg ctcttcatgg actgctcgcc gagcgcgaag cggaacgcct
     1801 ccgcgacgtt cgatttgccc gagccgttcg ggccgacgat cgcggtggta gcggacgcga
     1861 aatcgagcac ggtcttccgc gcaaaggatt tgaacccggc gatctcgagc cgcttcagca
     1921 tttatcgaaa gtataccgca tgacagtgtt cagggagcct taatctgcac tacggcaggc
     1981 tgcatgtgga gcaatttgag gagagaccag ccatggctga cggaaccact gccgctgccc
     2041 tgcagcggcc gagcgcgcga acgcttgccg aggcgcacga gcttctgaca cggatgacgc
     2101 ggatcaacgc ggatatccgt gccatcgagg aagaggcggc gatcaagacc gcggctgatc
     2161 gccgcgtcat cagcgaggcc gaggagcgga tagcaccggt ccttgccgag gcgaagctga
     2221 agctcgatcc gctcaagaag gaggcgcagg aagtcgggga ggggttccga cgcttcaccg
     2281 agcgccatcc gaagctgctg cccccgcccg cggaccgcct caagaccctg accttcaata
     2341 cgggccacac ctaccttcgg caggagatcg gattgaaggt ggtgaccaag ggcaagaacg
     2401 acgacgccgc gatcgaagag atcaaggcca aggggtggga ggcggacttc atcgagtaca
     2461 agccgttcct ccgcaggaac gagcttaagc gcaccgagca tcggcagcgc gtagaggagc
     2521 tcacctgcct cacgctgcgt atgacccgga agatcaccat cggcctgccg aacggcatcc
     2581 agttcatcgg caaggacgac gatcggggca agttcagctg ggaattcgaa tcctgacctt
     2641 tcacgtaccc tggcatgacg ccgcgagcct ccgggcttgc ggcgctcttg tttgtatacc
     2701 gaagccgccc cctgcggtac gcgagggggc ggaggggaag tactaaatcc cgacgggcgc
     2761 ctagacgaga tcgatgagcg cgcgtatgcc gacgccaagg caagagcaga ggatcagcca
     2821 ggccgcacgt ccaaagtgcc cgggatgctg ccggtagagc gcatagaatg cgagaccgac
     2881 ggcgacgatg aagcccccga ccgtcgtcag cacgaagacg atcgcagcga cgcgctgcag
     2941 gtcgccagtc caatgctcgg ccccgtagat cgagacccag ttcgggaccg cgacaccgac
     3001 gaaggtcagg gaggcgaagg aacggaggaa gcgcacgggt cacctatggg ttgtggttgc
     3061 cgatctatac tataccacta ccaccccgtc ttatcaagag ccgcctgcgc tgcagcctgc
     3121 tccgcctcct gcttgctctt cccttctcca cgcgcgatct cctcgtccgc gaggtacacg
     3181 ccgaccgtga actgcttatc gtggtccggg ccctcctcgg agagggtctg gtacgtcggg
     3241 gtgacgctcc gcttctcctg cgcggactct tggaactggc tcttcgcgtc ctggtatgca
     3301 cgggtcgcga tgacccgatc gatcttcggg tagaggtgct tcgccacgaa ctgctccgcc
     3361 gcctcgtatc cctggtcgag gtagatggcg ccgagcagcg cctcgaacgc attcgcgagg
     3421 atgatgtcgc gcgcgcggcc cgtatccttc ttctcgccct tggaaaggag gagcatgtcg
     3481 ttcaggccga tctccttcgc ggtatcggcg agcgagtagg tgttcacgag ggcggcgcgg
     3541 taggcggtga ggtccccttc cggcttgttc gggaagcgcg tgaagaggaa ctgggtcacg
     3601 gcgagctcga ggaccgcatc gccgaggaac tcgagccgct cgttgtggtt gccggcgccg
     3661 cggttctcgt tcaagtacga gcgatgggtc aaggcctcga cgaagagatc ggggttctgg
     3721 aaggagagtt cgagcttatc cgcgtagtcc ttgaactcgg tcatagacca gggactttac
     3781 gcccccttcc cggaccggtc aaggagcttc tgcacggccg ccgtcacgat gggcagcatg
     3841 tcgtcataga aattgaggtc gaggatatcc gggaggcgga accacttcgc atcatccaag
     3901 ccgccggtct tcttgagttc caccggttcg aactcgcttt tgacgaggaa gaaccagacg
     3961 tgcttcctga tcttcccctt ctcggggtgg ctcgccacgt actcgttctc gcccacctgc
     4021 tcttccaccg tcacgttctc gagcccgagc tcctccttca cgacgcgccg tacgccctcc
     4081 tcgggcgagg gatcgttctc gatatgcccc ttcgagaggg tccagcggcc gaagacgtcg
     4141 tgcacgagcg caaggtagta ctggccatcg tggtacgcgt agatgatggc gcccccgagc
     4201 ttctccaagg ggagcgcttc gcgcttcacc ttgtccttct tcccggtctc gtccttgccc
     4261 ggctccccga gctccttgta tacggcgccc aagacgccgt tgacgaaccg tcccgaggaa
     4321 tccccgccga acactttggc gagctcgatc gcttcgttga tcgcgacctt ggccggcacc
     4381 tgcgcgcgat ccgcgaacaa tagctcgtac agcccaatgc gcaggatgtt gcggtccacc
     4441 ggcgcgatgc ggtccagcgg ccaatccggc gccgccttcg tgatgatgag gtcgagctcc
     4501 tcctgcttcg cgagtacgcc tgcaagcagg gcttccatga atggccgatc ggtatcgtcc
     4561 ccgccgaatt cctcgacgtt gcgcgcgagc atctcggacg ctgcgctttg gtcggcatgc
     4621 gtcgtatccc actcaaagag cgtttgcagg acgacggatc gcgctaggtg ccggttggcc
     4681 atatggaaag aacgtgtaga tacaatacac ccgagttgat gaaagttcct agtagcgcgg
     4741 gatgtgcata aaacaaaaca ccggttgccc ggtgcgtttc gttaggcgcc gccgccgaat
     4801 cccgagcgac tcttggcctt gcccttggtc atgcggccga tgaggctctg cttcttctcg
     4861 atgccgtcgg cgaccgtgtg cttctcctcg tgcaccggct cgcgggcgcc gaggtccccc
     4921 tgctccgcct cgttcttctc gcggcgcttc ttctccacct tcacctcctg cttgcgctcc
     4981 gggacgatga gcttaccccg atacatgccg gtcgattcgt cgaggcggtg cgggaggcgg
     5041 tgcgcgccgc tctccttgtc ggtgaccacg gcgcctgccg ccaaagcgtg atggctccgg
     5101 cggttgcggg tgtgcgaacg ggtatggcgc atccgtactg acatagtgcg agtaccttac
     5161 catagggccc tttgcctggc tagcgccacg cagtcccctc tcttgccaaa cggctgagat
     5221 gtgggaatgt gaagatagct gctccttaga gggacctagg gtacgcagct gttcgctgcc
     5281 ccagttcggg gttgttgacg atcgggaaga aggattagca cccgatgcct atcgaagcga
     5341 atcttacctg taccgaccat agcaacgagg agagtacgac ggaatccagg gtggcggctg
     5401 ccgaacccac cgactacggg tagtgcttcc gcaccatcca atcccggcct tccgcctttg
     5461 agcgactgca gaccgcatct gctcgggcaa tgagaccgga gagggtcacg cacaccgcgt
     5521 ggccctcgtt aggttttata tgcgggtaag gaagctcctc cggtgctcga tgcagggccc
     5581 gaacgtacgg atcgcctcga tgtgcgcctt cgtgccgtat cccttgtgct tctcgaagcc
     5641 ataagcggga tattccttcg ccagcatctc catgtgcttg tcgcgggtca ccttcgccgc
     5701 gatcgacgcc agcatgatgg ccggaacgat gccatctccc ccgacgaccg tctcctgcgc
     5761 atattcggca ggcgccttca aggagccatc aaggaagacc ttcccctcat ggggattcgg
     5821 gaggagcttc tcgatgccgc gggcgaccgc atgccggatg gcgggtgcga tgccaccctg
     5881 atcgatgtag gtcgccccga cgagctgcac cgaggcgcgc acgttgcctg catgcatctc
     5941 cttctgcaga agctggaaga gcgcctcccg cttcttctca gagagcttct tggaatcgtt
     6001 caaaccgggg aaggcagcga ggaggtcgta cccgtcggcc accgcgatga cgccgaccac
     6061 gaccggtccg gcgagcggcc cgcgccctgc ttcgtcgaca ccaagaatga acttcatgca
     6121 aaaatgctac cacgcccata tttggtattc tgttagtaat ggctggccgc tacatccacc
     6181 tgcatacgca tagccactac tccttcctcc aggcgctccc gaaagtcccc gagctcgtta
     6241 aggccgcgaa ggccgccggc atggacgcgc tcgggcttac cgacgccggg aacctccacg
     6301 ccggcatcga gttctacaag gccgcggaga aagccggcat caaacccatc ctcggcgtgg
     6361 acgcgtacct cgcgcagggc tcgaggcgcc aggtgcacga ggtgccggcc gagcgcggcg
     6421 ggcgcacgcg gctcgtcctt ctcgcgatga acaacgaggg ataccagaac ctgctcaccc
     6481 tcatcaccgc atccttcacc gagggatacc acgaacgccc gcacatggat aaggagctgc
     6541 tgcgctcgca ccatgcgggc ctcatcgccc tcataccgtc tttcgcgggc gaggtcgcgc
     6601 agcacctgaa gcagggggac aagaagggcg cggcagccgc catcgaggag tacgtctcca
     6661 tcttcgggaa ggagaacgta tacctcgaga tcacacacca cccgaaggtg gaggggcacg
     6721 cgaagcgcat ggaggagatc aggaagctcg ccaaggagtc cggcgtgccg ctggtcgccc
     6781 agcacgatgt gtactacctc aagccttcgg accgcgaggc caccgaggtc atgcgccgca
     6841 tcgcgcatgg cgagcgcggg cgaaacgagg atgaggattt ctccttcgta tccgagagcc
     6901 agatgcgcga atggttcgcg gacgtcccgg aggcggtcga tgcgtcaggc gagatcgcgg
     6961 accgctgctc catccatttc gagctcggga gctggaactt ccccgcggtc gaggtcgagc
     7021 ccgggaaaac gcatgaggag atgctccggg agcgcaccta cgacgggctg gccatgcgcg
     7081 gcatggacaa gacgaa
//
LOCUS       DASPKG010000012         3094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_151976, whole genome shotgun sequence.
ACCESSION   DASPKG010000012 DASPKG010000000
VERSION     DASPKG010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151976"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VEA36_00510"
     CDS             complement(<1..590)
                     /locus_tag="VEA36_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28834.1"
                     /translation="MSVLKRIACIVIVIFALIGVAFTLVFIAMQFGLLNVRGSIMERN
                     QFFVSAEASVPEDRSCPDAAETCAWNQTREWAAVEGGLRKDAHVIERVARETGVPARL
                     IAAAVIPEQLRFFTSEREVFKRYFEPLKILGSLSQFSLGVSGIKQETAEAVERHARDA
                     TSPFYPGPTYAAYLAYPAGVDESAALYSRLTDPKDH"
     gene            640..727
                     /locus_tag="VEA36_00515"
     tRNA            640..727
                     /locus_tag="VEA36_00515"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:674..676,aa:Ser,seq:gct)
     gene            complement(737..1327)
                     /locus_tag="VEA36_00520"
     CDS             complement(737..1327)
                     /locus_tag="VEA36_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28835.1"
                     /translation="MSVLTRLLDLLFPPRESATLVAGATINDLHRRYAPGTYVVDGAR
                     IMYLLPYRDPLVRACVVEAKFHGNTRAQGLLGRMLEDYAGTTDAVLVPIPLSARRLRA
                     RGYNQVEEIAKARCAGRVDTTVLQRTRDTRAQTAAQEREERLRNLTGAFSATVDPAKR
                     YLVVDDVATTGATLSDAHRALREAGAKYIELLAIAH"
     gene            1350..2438
                     /gene="pilM"
                     /locus_tag="VEA36_00525"
     CDS             1350..2438
                     /gene="pilM"
                     /locus_tag="VEA36_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HYF28836.1"
                     /translation="MSFFSKLFASTKKSKSVIGIDVGSSSLKVVQLRRDGGTAILETY
                     GELALGPYSGGEVGTATNLPAAKIAESLKDLLREANVTSTDAGVSIPFSRSLLTLVSL
                     PYRESADEQKTVIELEARKYIPVPVSEVQLDWFILPQPIPEGQQAPQKVDVLLVAVHN
                     DELKLLEQVVNEAQLSATFYEIEIFSTIRSVVDEPVKPVLVLDIGASATKVYVVEHGV
                     VALSHNIPKGGQDVTRTIATAHGMPIAKAEILKKEHGFQGGTDAYDRKTIELVFSRIF
                     EEAKRAMTQYETAAGKAISALILTGGGGVTKELGQYAQTFFSAPVQLADPFSKAEAPA
                     FMQPVLKEIGPEFAVAVGLALRKLEELE"
     gene            2492..>3094
                     /locus_tag="VEA36_00530"
     CDS             2492..>3094
                     /locus_tag="VEA36_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28837.1"
                     /translation="MALPPTVPTSFVPKQPVQTTRRAKRSGNLFFTVSLVVMALAIAG
                     AGATFVYQYVLESIRDNKAKELAAVEATTNRATIDEFIRLRDRLVASRTILDGHVALS
                     QYFDVLEALTLKTVSYDSLELVEAEGGRIELTMNGEARTFNTLAAQSAAFASEKRIKR
                     AIFSDIQVTQSGRVSFSLSAELAPELIRWQAPVAALPVAPS"
BASE COUNT          537 a         1023 c         1004 g          530 t
ORIGIN      
        1 tagtgatcct tcgggtcggt gaggcgggag tagagcgctg cggattcgtc gacgcccgcg
       61 gggtaggcga ggtacgccgc gtaggtcggt ccgggataga agggcgaggt cgcgtccctg
      121 gcatgccgct ccaccgcctc cgccgtctcc tgcttgatgc ccgagacgcc gagcgagaac
      181 tgcgagagcg agccgaggat cttcaggggc tcgaagtatc gcttgaacac ctcgcgctcg
      241 gacgtgaaga agcgcagctg ctcgggaatg accgcggccg cgatgaggcg cgcagggacg
      301 ccggtctcgc gcgctacccg ttcgatgacg tgcgcgtcct ttcgcaggcc gccctcgacc
      361 gccgcccact cccgcgtctg gttccaggcg caggtctctg ccgcatcggg gcaggaccga
      421 tcctcgggca ccgacgcctc cgcgctcacg aagaactggt tgcgctccat gatggagccg
      481 cggacgttca ggagcccgaa ctgcatcgcg atgaagacga gcgtgaacgc gacgccgatg
      541 agcgcgaaga tgacgatgac gatgcacgca attctcttga gtacggacat ggacggatta
      601 ttttgaaacg cacctcccat ttgctatgct cgccctacgg gagacgtggc tgagtggtcg
      661 aaagcgcctc actgctaacg aggtaggggt taatagcccc tccagggttc gaatccctgc
      721 gtctccgcat tctgcatcag tgcgcgatcg caagcaactc aatgtacttc gcacccgctt
      781 cccggagcgc tcgatgcgca tcagagagcg tcgcgccggt ggtcgcgaca tcgtccacga
      841 cgaggtaccg cttcgcggga tccacggtcg cgctgaacgc gccggtcagg ttgcgcaatc
      901 gttcctcacg ctcctgcgca gcggtctgcg cccgcgtatc gcgggttctc tggagcaccg
      961 tcgtgtccac gcgacccgca cacctggcct tcgcgatctc ctccacctgg ttatacccgc
     1021 gtgcacgcag ccggcgtgcg ctgagcggga tggggacgag cacggcatcg gtcgttcccg
     1081 catagtcctc gagcatccgg ccgagtagtc cctgcgccct cgtgttccca tggaacttcg
     1141 cctcgacgac gcaggcgcgc accagcgggt cgcggtacgg gaggaggtac atgattcggg
     1201 caccgtcgac gacgtacgtg ccgggagcgt agcgccggtg caggtcgttg atcgtcgcgc
     1261 ccgcgacaag cgtcgcgctc tcccgcggcg ggaagaggag gtcgaggagg cgcgtaagga
     1321 cggacatcat cgcatgatac aatcgccgca tgagcttttt ctcgaagctg ttcgcgagca
     1381 cgaagaagag caagagtgtc atcggcatcg acgtcggctc atcgtcgctg aaggtcgtgc
     1441 agctccggcg cgacggcggc accgccatcc tcgagacgta cggcgagctc gcgctcggcc
     1501 cgtactccgg cggggaggtg ggcaccgcga cgaacctgcc ggccgcgaag atcgcggagt
     1561 cgctcaagga cctgctgcgg gaggcgaacg tgacctccac cgatgcgggc gtctccatcc
     1621 cgttctcgcg ctctttgctt accctcgttt cgttgccgta ccgcgagagc gcggacgagc
     1681 agaagacggt gatcgagctc gaggcgcgca agtacatccc cgtgcctgtc tccgaggtgc
     1741 agctcgactg gttcatcctc ccgcagccca tccccgaggg gcagcaggcg ccgcagaagg
     1801 tggacgtgct gcttgtcgcg gtgcataacg acgagctgaa gttgctcgag caggtcgtga
     1861 acgaggcgca gctctccgcg accttctacg agatcgagat cttcagcacc atccgctccg
     1921 ttgtagacga gccggtgaag ccggtgctcg tcctcgacat cggggcgtcg gcgaccaagg
     1981 tatacgtcgt cgagcacggc gtcgtcgcgc tctcgcacaa catcccgaag ggcggccagg
     2041 acgtgacccg caccatcgcg accgcccacg gcatgccgat cgccaaggcg gagatcctca
     2101 agaaggagca cggctttcaa ggagggaccg atgcgtacga ccgcaagacg atcgagctcg
     2161 tcttctcgcg catcttcgag gaggccaagc gcgcgatgac gcagtacgag acggcggcag
     2221 ggaaggcgat ctccgcgctc atcctcaccg gcggcggcgg cgtcacgaag gagctcggcc
     2281 aatacgcaca gaccttcttc tcggcgccgg tccagctcgc cgaccccttc agcaaggcgg
     2341 aggcgcctgc cttcatgcag ccggtcctga aggagatcgg cccggagttc gcggtcgcgg
     2401 tggggctcgc gctgcgcaag ctcgaggagc tcgaataata cgcttgtgta taagcggcgc
     2461 gtattgattt tacgcaccgt atactgacat cgtggccctt cctccaaccg tacccacgtc
     2521 attcgtcccc aagcagcccg tccagacgac gcggcgcgcg aagcgttcgg ggaatctctt
     2581 cttcacggta tcgctcgttg tcatggcgct cgcgatcgcc ggcgccggcg cgaccttcgt
     2641 ctaccagtac gtgctcgagt ccatccggga caacaaggct aaggagctcg cggcggtcga
     2701 ggcgacgacc aaccgcgcaa ccattgatga gttcatccgg ctgcgcgacc ggctcgtcgc
     2761 gagccggacc atcctcgacg gccacgtcgc gctctcgcag tacttcgacg tgctcgaggc
     2821 gctcaccctg aagacggttt cctacgattc cctcgagctc gtcgaggccg agggcggtag
     2881 gatcgagctg acgatgaacg gggaagcgcg gaccttcaac acgctcgcgg cgcaatcggc
     2941 ggccttcgcg tccgagaagc gcattaagcg ggccatcttt tcggacatcc aggtgacgca
     3001 gtccggccgc gtctcgttct cgctctccgc cgagctcgcg cccgagctca tccgctggca
     3061 ggcgccggtt gcggcgctcc cagttgctcc gagc
//
LOCUS       DASPKG010000013         4954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_423058, whole genome shotgun sequence.
ACCESSION   DASPKG010000013 DASPKG010000000
VERSION     DASPKG010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423058"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VEA36_00535"
     CDS             complement(<1..347)
                     /locus_tag="VEA36_00535"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYF28838.1"
                     /translation="MEDLIRPIAEAAIKERTFPGCVVGIIKRNGERMVLPFGRFTYED
                     SAPAVRENTLYDLASITKSIPTGSLALQLIDQGKLRITDKLIDHIPEFNNSDRDTVLV
                     KHLLTYTLDGYGFA"
     gene            complement(434..1021)
                     /locus_tag="VEA36_00540"
     CDS             complement(434..1021)
                     /locus_tag="VEA36_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28839.1"
                     /translation="MAVISIVGTSGVGKSFLVTQLASLDCMPAFLEGEEGVIPQEVFT
                     NIFAGDPVGRYAYFIERYNHNLSRARRISDLGLDAYVDGATLSPHAILLYEEPQYHEP
                     IRDITKSMAGNESDVIVLLTASEEKIAQGLEVRGRSSEATAEALRRAIAIQGYFEAEA
                     AKLDNAITVDRTGLDFKDEKQLRDIAAGIKDFLKR"
     gene            complement(1025..1657)
                     /locus_tag="VEA36_00545"
     CDS             complement(1025..1657)
                     /locus_tag="VEA36_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYF28840.1"
                     /translation="MVYNPNKFPTRPERQDIPPLSFAELSPDTWNALAELPRAGWVKR
                     GVENPESVQEHIIALRRVASTLADIPEADMRDLLDMLEVHDWPEAIHGDEIIAGAVPE
                     ERQKLEEAKFEKEQQALVSICEGLGPAGQRIVELWLRFEKGEDEIASLARQLDKFQAV
                     AKALEYEKASGKLIFLDFYTYAQNHVTHPQLVDRMEKLKEEWGMHNENND"
     gene            complement(1775..2023)
                     /locus_tag="VEA36_00550"
     CDS             complement(1775..2023)
                     /locus_tag="VEA36_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28841.1"
                     /translation="MLEERDHILIPNLDDSRVDRIHNLEDDGVGEESLRQARALAEER
                     GITCEPSGIAGLALLLQKQDEIPRDKKILIVNTGKLVL"
     gene            complement(2117..2190)
                     /locus_tag="VEA36_00555"
     tRNA            complement(2117..2190)
                     /locus_tag="VEA36_00555"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2154..2156),aa:Asp,seq:gtc)
     gene            complement(2181..2861)
                     /locus_tag="VEA36_00560"
     CDS             complement(2181..2861)
                     /locus_tag="VEA36_00560"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HYF28842.1"
                     /translation="MTMVRTLNHEFFKTWTPEMAYVLGYFAADGSLNTGHGGSYIEFK
                     SVDRKMIENVREAIGSSHRISQYERGGNCKTCYRLQISSRVWARDLQGFGFTPKKSKT
                     MRFPLVPAEYIGHFVRGYFDGDGCVYSQMLKYADRDHKRWILLTLFTSGSKVFLEQLW
                     SILKDHGIRGGSLVPKKRGFELKFSHRDSLALYQLMYHTAPISDLYLLRKRAKLEQAI
                     QVLDLLRA"
     gene            complement(2908..2981)
                     /locus_tag="VEA36_00565"
     tRNA            complement(2908..2981)
                     /locus_tag="VEA36_00565"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2945..2947),aa:Met,seq:cat)
     gene            complement(2993..3706)
                     /locus_tag="VEA36_00570"
     CDS             complement(2993..3706)
                     /locus_tag="VEA36_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HYF28843.1"
                     /translation="MQALILASGRGTRMGTLTDVLPKPMLTVAGKTLIDHKLEALPDA
                     VDEVILVIGYLGHTIRERYSDSSHGRRIRYVEQQDLAGTGAAAWLARPLIEGRFLVLM
                     GDDLYGREDIERCIALPDWSVLVERTQTMAAGGSMVLDGAGRVLGIEEGDHSGRPGIM
                     NTNMFVLDERVFEQSLVPKAPGSAEFGLPQTVVEASRNLNVPLTAVNATAWIQITAPE
                     DLLIAERRLAEIEGKGKAV"
     gene            3859..4527
                     /locus_tag="VEA36_00575"
     CDS             3859..4527
                     /locus_tag="VEA36_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYF28844.1"
                     /translation="MRILVVEDEEKIANFLKESFEADFFAVDVARDGEQGSYLARIND
                     YDLIVLDNLLPKKEGAQVCRDIRTAGKQVPILIVSVRTSTDDKVDLLDAGADDYLTKP
                     FALEELMARVRALLRRPPELEGDVLQIEDIILDSKTGTVHKGTKELHLTRKEFMLLQY
                     LMRNAGIVLSRGMILEHVWDMNVDIFSNTIESHIMSLRKKVGDRGRNRLIQTISGRGY
                     RMGT"
     gene            4629..>4954
                     /locus_tag="VEA36_00580"
     CDS             4629..>4954
                     /locus_tag="VEA36_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020365844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HYF28845.1"
                     /translation="MTVSPEVITGTELPEHPDLTKIYRLLPNDEDPAYYAERVDRNIG
                     WLTEAEQYALFGKVVGIAGCGGMGGAIAQSLLRLGVGEIRIADPEVFDISNINRQFAA
                     RRDSIG"
BASE COUNT         1080 a         1470 c         1272 g         1132 t
ORIGIN      
        1 gcgaatccat acccgtcgag cgtataggtg agcaggtgct taaccaggac agtgtcgcga
       61 tcagaattat tgaactcggg aatgtgatcg atgagcttgt cggtaatgcg tagcttccct
      121 tgatcgatca gctgcagagc aaggctcccc gttggaatgg atttggtaat agaggcgagg
      181 tcgtagagtg tgttctccct aactgcaggc gcactgtcct cgtacgtgaa tcggccgaac
      241 gggagaacca tccgctcccc atttctcttt atgattccaa cgacgcaccc cgggaaggtc
      301 cgttccttga tggctgcctc tgcgattggc ctgatcagat cttccactgc tccagtatag
      361 ctggttcgat attctttgga cggaatcgca aaaccgccca actgggcggt ttctggagtg
      421 ccgcactcgt cgattatctc ttcaggaaat cctttatgcc cgcagcgata tcgcgaagct
      481 gcttctcgtc cttaaaatcc aagcccgtac ggtcaaccgt aatcgcattg tccagctttg
      541 ctgcctctgc ctcaaagtac ccctgtatgg caatagccct tctgagggcc tccgcggtag
      601 cttctgagct ccggccccgc acttccagac cctgtgctat cttctcctcg cttgcagtca
      661 ggagcacgat aacgtcggat tcgtttcccg ccatcgactt cgttatatcc cgtataggct
      721 cgtgatactg cggctcttca tacagaagga ttgcatgggg cgataacgta gcgccgtcta
      781 cgtatgcatc gagacccaaa tctgaaatac ggcgcgcgcg ggacaggttg tgattgtatc
      841 gttcgatgaa gtacgcatat cttccgacgg gatcgcccgc gaagatgttc gtgaatacct
      901 cttggggaat aaccccttcc tcgccctcaa gaaaggcggg catgcaatcg agcgaggcga
      961 gctgggtcac caagaaggac tttccgactc ctgacgtccc gacaattgag attacggcca
     1021 taccttagtc attattctcg ttatgcatac cccactcctc tttcagcttc tccattcggt
     1081 ccactaactg aggatgcgtt acgtgatttt gggcataggt ataaaaatca aggaagataa
     1141 gcttacccga tgccttttca tattccaacg ccttcgccac cgcctggaat ttatccagct
     1201 gacgtgcaag agacgctatt tcgtcctcgc ccttttcgaa tcgcaaccat aactcaacaa
     1261 tcctctgacc cgcaggaccc agtccctcgc atatggacac aagcgcctgc tgttcctttt
     1321 caaatttagc ttcctcgagc ttctgccgct cttcgggaac agcgccagcg atgatctcat
     1381 ccccatgaat ggcttctggc cagtcgtgga cctccaacat atcgagcaaa tcacgcatat
     1441 ccgcctcagg aatatcagca agggttgaag caacgcgtct caaggcaata atatgctcct
     1501 gaacagactc ggggttctct acgcccctct tcacccagcc tgccctcggc aattcagcga
     1561 gcgcattcca cgtatcgggt gatagctccg caaacgaaag cggcgggatg tcctggcgtt
     1621 caggtcgagt cgggaattta ttcgggttat agaccacacg ggaagtatac cttccggtat
     1681 aaaggttcag tagccaccgc ctggacggag cttttatggt ttgcgcggtc cgtcgaccct
     1741 gttaaaactg ctatctcaac ccgcgaaggg accgctagag aacaagcttc ccggtgttta
     1801 ctatgaggat tttcttatct cgcggaatct catcctgctt ctggagaagg agcgcgagtc
     1861 ccgcgatgcc agaaggctcg caggtaatgc cccgctcctc ggccaaagcc cttgcttgtc
     1921 ttaacgactc ctccccgaca ccgtcatcct ccaggttatg gatcctatcc accctagaat
     1981 catcgagatt agggataagg atatggtcac gctcttcaag cactacttcg atgcgatttg
     2041 ggaggaagca gttccggtaa ctgtgtaaac agaaagaccg cccctacgag cggtctttct
     2101 gcacatctga actttgcgcg agcgacggga cttgaacccg caacctctgc cgtgacaggg
     2161 cagcgctcta accagttgag ctacgcccgc agtaaatcca agacctgtat tgcctgctcg
     2221 agcttggcgc gcttccggag cagatacagg tcggatatag gcgcagtatg atacatcaat
     2281 tggtagagtg caagactgtc tcgatgactg aatttaagct caaagccacg tttcttaggg
     2341 accagtgagc caccacgtat cccgtgatcc tttaaaatgc tccatagctg ttcaagaaag
     2401 actttggatc cgcttgtaaa aagcgttagc agaatccagc gcttgtggtc acgatcagcg
     2461 tatttgagca tctgagagta aacgcaaccg tcaccgtcaa agtatccacg tacgaagtga
     2521 ccgatatact cggcaggcac aagcggaaat ctcattgtct tgctcttctt gggagtaaat
     2581 ccaaaccctt gcaagtccct tgcccagacc ctgctcgata tctgaagccg atagcatgtt
     2641 ttacaattgc caccccgctc gtattgagat atgcgatgag aagagcctat cgcttcacga
     2701 acgttctcga tcatttttct atcaacactc ttaaattcga tgtaactccc gccatgaccc
     2761 gtgtttagcg atccatccgc agcaaagtac cccaatacat acgccatttc aggcgtccat
     2821 gttttgaaga attcatggtt gagtgtgcgt accatcgtca tggcagcagc ctagctcaac
     2881 cacatcaaat ttccctgaag ccaattgtgc tggtgggagg acttgcacct ccgaccttgg
     2941 gtttatgagt cccatgctct aactacctga gctacaccag cgtattagga cattacactg
     3001 ccttgccctt cccttcaatc tcggcgaggc gtcgttcggc gatgaggaga tcctcaggcg
     3061 ccgtgatctg gatccatgcc gtagcgttga ccgcggtaag cgggacgttg agattgcgag
     3121 atgcctcaac caccgtctgc ggcaggccga actcggcgct ccccggcgcc ttcgggacga
     3181 gcgattgctc gaacacgcgt tcgtcgagca cgaacatgtt cgtgttcatg atgcccgggc
     3241 gtcccgaatg atccccttcc tcgatgccga ggacgcggcc ggcgccgtcg agcaccatgc
     3301 tcccgccggc agccatggtc tgcgtccgtt caacgagcac cgaccagtca ggaagcgcga
     3361 tgcagcgctc gatgtcctcc cggccataga gatcgtctcc catgaggacg aggaagcgac
     3421 cctcgatgag cggccgagcg agccatgccg cggctccagt acccgcgagg tcctgttgtt
     3481 caacgtacct gatacgccgt ccgtgcgagg aatcgctgta tcgctcccgg atcgtgtggc
     3541 cgaggtaccc gatcacgaga atgacctcat cgaccgcatc cggaagcgct tcgagcttat
     3601 ggtcgatgag ggtcttcccc gccacggtga gcatgggttt tgggaggacg tcggtgagcg
     3661 tacccatccg cgtgccgcgg ccggatgcga gtatgagtgc ctgcatgctc atgcatgata
     3721 gcgagcccgg cacctaatgt gaaatcttgc tggagttttg atagccaaaa gataaacatt
     3781 tcgacacagt tttacacatg tgcaagactt ccgattcccc ccgccctgct acaatgcggg
     3841 ggaccgtagc gatggaccat gagaatctta gttgtcgagg atgaggagaa gatcgcgaac
     3901 ttcttgaagg aatccttcga ggcggacttt tttgccgtcg atgtcgcgcg cgacggcgag
     3961 cagggctcct acctcgcacg catcaatgat tacgatctca tcgtgctcga taacctcctc
     4021 ccgaagaagg aaggcgcgca ggtctgccgc gacatccgca ctgctggcaa gcaggtcccc
     4081 atcctgattg tctcggtgcg caccagcacc gacgataagg tcgatctgct tgatgcaggc
     4141 gccgacgact acctcacgaa gccctttgcg ctcgaggagc tcatggcccg ggtccgggct
     4201 ctgctgcgcc gtccgcccga gctcgagggt gacgtgctcc agatcgagga cattatcctc
     4261 gactccaaga ccggcaccgt acataagggt acgaaggagc tccaccttac ccgtaaggaa
     4321 ttcatgctcc tgcagtacct gatgcgtaat gccggcatcg tgctctcgcg cggcatgatc
     4381 ctcgagcacg tctgggatat gaacgtggac attttctcca atacgatcga atcccacatc
     4441 atgtcgctcc ggaagaaggt tggcgaccgc ggccgcaacc gcctcatcca aaccatctcc
     4501 ggccgcggct accgcatggg cacctagccc agcttttcat catcttttct ctaggaaaat
     4561 ttcatcttcg gcctctacta tgcagctagt accctgtaca ttccagcatc atcgaaagga
     4621 acgtccccat gaccgtctcc cccgaggtca tcaccggtac cgagcttcct gagcatcccg
     4681 acctgacgaa gatctaccgt ctcctcccga acgacgagga tcccgcctac tacgcggagc
     4741 gcgtcgaccg taacatcggc tggctgaccg aagccgagca gtacgcgctc tttggcaagg
     4801 tggtcggcat cgccggatgc ggcggtatgg gtggcgccat cgcccagagc ctgttgcggc
     4861 tcggcgtcgg cgagatccgc atcgccgatc ctgaggtctt cgatatatcg aacatcaacc
     4921 ggcagttcgc ggcgcgccgc gattcgatcg gcga
//
LOCUS       DASPKG010000014         5018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_288631, whole genome shotgun sequence.
ACCESSION   DASPKG010000014 DASPKG010000000
VERSION     DASPKG010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288631"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VEA36_00585"
     CDS             complement(<1..358)
                     /locus_tag="VEA36_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYF28846.1"
                     /translation="MADAFDRSKPHMNVGTIGHVDHGKTTLTASILNVLNLNSDKGYK
                     ARVEKVENIDNAPEEKARGITIALHHSEYESPNRHYAHIDAPGHADYIKDMITGAAQM
                     DDAVLVVAATDGAMPQT"
     gene            complement(564..1070)
                     /locus_tag="VEA36_00590"
     CDS             complement(564..1070)
                     /locus_tag="VEA36_00590"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYF28847.1"
                     /translation="MARPSSRGFTLIELLVVIAIIALLATIVLASLNTARMRASTEAV
                     KSQIVKMHAPAELIHQTTGSYLTVCDANTNTGRMFRAAFEHGSRADDQTRCLAQHGTS
                     YRYYTSSGGTFTQTNGTAGSSAQRWAAVVQLRTGWWFCFDSEGRFLEQTSPGVYASGP
                     VVGASCTF"
     gene            complement(1148..1483)
                     /locus_tag="VEA36_00595"
     CDS             complement(1148..1483)
                     /locus_tag="VEA36_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28848.1"
                     /translation="MQDIQAFAANVNAVRCGRVTTNPKRFLFNLDPPIVYEVPDDPVQ
                     VREGVEFARQAGIDILAYAAHRSDFNERTVYWNRGHPQRPRSAIGWITLEAWLAEAEA
                     AVSVPLAAE"
     gene            1544..1921
                     /locus_tag="VEA36_00600"
     CDS             1544..1921
                     /locus_tag="VEA36_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28849.1"
                     /translation="MKREEAAAYATARSAPPLPQPNDVPEILRRAAGPLLPRRLRDPL
                     LLALPVRLLEGTRTHDFERTSVPLAARDELALRERRRWRLGIERVVAGSPVEVEEYVR
                     SREQHNDVVAPLEVKEGDASHPE"
     gene            complement(1979..2929)
                     /locus_tag="VEA36_00605"
     CDS             complement(1979..2929)
                     /locus_tag="VEA36_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28850.1"
                     /translation="MDTPTSFPGPVTLLKAGLSHYKQRGRAVLTIAIAQFVIGMALGF
                     AIVMLSALGTVGMLIGVPLALLFVWAVLWLGLAMLMVFVAEPGSLSARAALGRAAPQV
                     FRFLWVNVLTVLVYSGILALVAGIPALVLYFVAGPVPALVAGLVLFFVAAIVTSPWFS
                     FVGWAFADQGARGMQALLTSREMVRGRNWSVLWRMVVIGFLVGIATVILVIPFELIAL
                     ALPGVVGDILSEIGSGIAQVLLAPIFVGALYALYAHLKPVAGAPTPVGNGKRYTLLAI
                     LGVLFVPAFFGFIAYLIMTQAPMLETAGEFESEPLSAPVE"
     gene            complement(2996..>5018)
                     /gene="fusA"
                     /locus_tag="VEA36_00610"
     CDS             complement(2996..>5018)
                     /gene="fusA"
                     /locus_tag="VEA36_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HYF28851.1"
                     /translation="VHEGETTTDWTEQERERGITITAAAITCFWTKTEEKDKKDPAKK
                     YRFNVIDTPGHIDFTAEVKRSMRVLDGAIVVFDGVAGVEPQSETNWRYADEAGVPRVC
                     FINKLDRTGASFEDSYKSILDRLSKKAVRAQIPMGAESDFEGVIDLLAMKSYTFTGNM
                     GEDVVEGEVPEQYMEEAKKYRAELIERIVEHDDALMAAYFEGTEPSLEDLKKTLRKAV
                     IANEIFPVYTGSALKNKGVQLVLDAVVDYLPSPLDLPPVKGTNPKTGEEVERRPDDSE
                     PFTALAFKLQTDPFVGALTFFRVYAGTVSAGSYIYNANTGTKERVGRIVRLQADKREE
                     VEQVFTGEIAAFVGMKDTKTSHTLCDEANPIVLEQIVFPEPVVSLRIEPKTKADQEKM
                     GMALKKLSDEDPTFKISSDEETAETIISGMGELHLEILVDRMQREFNVGANVGKPQVA
                     YRETVMGTADVENKYIKQTGGKGQYGHVKIKLKHLEPIDPDAKIPKNVTREDHFEFIN
                     NIKGGVIPQEYIPAVEKGIREALNRGILAGYPIVDVSVDLYDGSYHDVDSSEIAFKIA
                     ASQAFQEAAQKAKPVILEPIMKVEVVVPEQFMGDITGNLSGKRGAIEGMEDRGMNKAI
                     NAKVPLSEMFGYTTTLRSMTEGRGSMTMEFDHYEVVPANVAQEIIAARK"
BASE COUNT          905 a         1570 c         1605 g          938 t
ORIGIN      
        1 gggtctgcgg catggcgccg tcggttgcgg cgaccacgag gacggcgtcg tccatctggg
       61 cggcacccgt gatcatgtcc ttgatgtagt cggcgtggcc cggcgcgtcg atgtgcgcgt
      121 agtggcggtt cggcgactcg tactccgagt gatggagcgc gatggtaata ccacgagcct
      181 tctcttcagg cgcgttgtcg atgttctcga ccttctcgac gcgggccttg tagcccttgt
      241 cggagttgag attaagcacg ttgaggatcg aagccgtgag cgtggtctta ccatggtcga
      301 cgtgaccgat ggtacctacg ttcatgtgcg gcttgctgcg atcgaatgca tctgccatag
      361 gtactgaaat tacttggtag ctacgaataa ttcgcgccgc gcctgcctgg cttcgtaaga
      421 caggcgacca aggcggtagg cgtatccccg cgtagcgcct tctagccacc cctcctatgg
      481 agaaggaata ctaaaaagag cgccgtactc gcgttagggg taaaggtaca cgggcgctcg
      541 tgaagcgtca aggaatgacg ggcctagaag gtgcagctcg cgccgacgac gggcccgctc
      601 gcgtacacgc cgggcgaagt ctgctcaagg aatcggccct ccgaatcgaa gcagaaccac
      661 caaccggtcc gcagctgtac cacggcagcc caccgctgcg ccgaactgcc tgcggtcccg
      721 ttcgtctggg taaaggtgcc gcccgaggaa gtgtagtacc ggtagctcgt gccgtgctgg
      781 gcaaggcagc gggtctggtc gtctgctctc gagccgtgct caaacgcggc gcggaacatc
      841 cggcccgtat tcgtgtttgc atcgcacacc gtgagatagc tccccgtcgt ctgatggatg
      901 agctccgcgg gcgcatgcat ctttacgatc tgggatttga ccgcttcggt gcttgcgcgc
      961 atgcgggcag tattgagcga tgcgagaacg atggtcgcga gaagcgcgat gatcgcgatc
     1021 actaccagga gctcgatgag cgtgaagcca cgcgatgaag ggcgagccat ggcgcaacga
     1081 tagcatgtga aagccccgcc gattgctcgg cggggcgtgc acgttcgacg ttcgtgcagc
     1141 gtgcaactta ttccgcggcg agcgggacgg aaacggcagc ctccgcctca gccagccacg
     1201 cctcgagcgt gatccagccg atggcgctgc gcggacgctg cggatgaccg cggttccagt
     1261 aaaccgtgcg ctcgttgaag tcgctgcggt gcgccgcgta cgcgaggata tcgatgcctg
     1321 cctgccgcgc gaactcgacc ccctcgcgga cctgcaccgg atcgtccggc acttcgtaga
     1381 cgattggcgg atcgagattg aacaaaaaac gcttcggatt cgtcgtgacc cgcccgcacc
     1441 ggacggcatt cacattggcg gcaaaggctt ggatatcctg cacggattca ctcctgatag
     1501 aactgggctg tgtctgtaat accgcgcgct agctgtacgg cgcatgaaac gcgaggaggc
     1561 ggccgcatac gcgaccgccc gttctgcccc acccttaccg caaccgaacg atgtgccgga
     1621 aattcttcgg cgcgccgcgg gacccttgct gccgcgaagg cttcgggatc cgctgctgct
     1681 cgcgctgcca gtgcggctcc tcgaaggcac gcgcacccat gacttcgagc gtaccagcgt
     1741 accgctggcc gcccgcgatg agctcgcgct cagggagcgc aggcggtgga ggctcggtat
     1801 cgaacgtgtg gtcgccggct ccccagttga ggtcgaggaa tacgtccgga gccgagaaca
     1861 acacaacgac gtggtcgcgc cgctcgaggt caaggagggc gatgcatccc atcccgaata
     1921 gccctacgat ggtcgccatg gcggcgtcct ccctgtctga tctgccgcaa cgatagcact
     1981 actcgacggg tgcggaaagc ggctcggact cgaactcccc agccgtctcg agcatcggcg
     2041 cctgcgtcat gatgaggtat gcgatgaagc cgaagaaggc ggggacgaag agcacgccga
     2101 ggatcgcaag cagcgtgtag cgcttgccgt ttccgaccgg cgtcggcgct cccgcgacgg
     2161 gcttcaggtg cgcgtagagc gcatagaggg cgccgacgaa gataggcgca aggagcacct
     2221 gcgcgatccc gctcccgatc tcgctcagga tatccccgac gacgccggga agcgcgagcg
     2281 cgatgagctc gaatgggatg acgaggatga ccgtcgcgat gccgacgagg aatccgatga
     2341 cgaccatccg ccacagcacg ctccagttgc gcccgcggac catttcgcgg cttgtaagca
     2401 gggcctgcat gccgcgtgct ccctgatccg cgaacgccca gcccacgaag gaaaaccagg
     2461 gcgacgtgac gattgccgcg acgaagaaga ggacgaggcc agcaacgagc gcggggacag
     2521 ggcccgcgac gaagtacaag acgagtgccg ggatgcccgc gacaagggcg aggatgccgc
     2581 tgtatacgag cacggtaagc acattcaccc agaggaaccg gaagacctgc ggtgcagcgc
     2641 gcccgagcgc agcgcgcgcc gagagactcc ccggctcggc cacgaacacc atgagcatgg
     2701 caagcccgag ccagagcacg gcccagacaa agaggagcgc gagcggcacg ccgatgagca
     2761 tcccgaccgt gccgagtgcg ctgagcatta cgatcgcgaa gccgagggcc atcccgatca
     2821 cgaactgcgc gatcgcgatg gtgagcacag ccctcccgcg ctgcttatag tggcttagac
     2881 cggccttcag gagagtgacc gggcccggaa acgacgtagg agtatccata tatccgcacc
     2941 atagcacgaa agccgcttca tgaagcggct ttcggcaggc ccgtggataa cccctctatt
     3001 tccgcgccgc gatgatctcc tgggcgacgt tagccggcac gacctcgtag tggtcgaact
     3061 ccatggtcat ggacccgcgt ccttccgtca tggatcgaag cgtcgtggtg tagccgaaca
     3121 tctcagagag cggcaccttg gcattgatcg ccttgttcat gccgcggtcc tccatgccct
     3181 cgatcgcgcc gcgcttcccg gagaggttgc ccgtgatgtc gcccatgaat tgctcgggca
     3241 ccaccacctc gaccttcatg atcggctcga ggatgacggg cttggctttc tgcgccgcct
     3301 cctggaaggc ctgggaggcc gcgatcttga aggcgatctc cgaggagtcc acgtcgtggt
     3361 acgagccgtc gtagaggtcg accgagacgt ccacgatcgg atagcccgcg agaatgcctc
     3421 gattcaatgc ctcgcggatg cccttctcca ccgcggggat gtattcctgc gggatgacgc
     3481 cgcccttgat gttgttgatg aactcgaagt ggtcctcgcg ggtgacgttc ttcgggatct
     3541 tggcgtccgg atcgatgggc tcgaggtgct tcagcttgat cttcacgtgc ccgtactgac
     3601 ccttgccgcc ggtctgcttg atgtacttgt tctcgacgtc tgcggtgccc ataacggtct
     3661 cgcggtacgc cacctgcggc ttgcccacgt tcgcgcccac gttgaactcg cgctgcatgc
     3721 gatccacgag gatctcgagg tggagctcgc ccatgccgga gatgatggtc tcggcggtct
     3781 cctcatctga tgagatcttg aaggtcgggt cctcgtcgga gagcttcttc aaggccatgc
     3841 ccatcttctc ctggtctgcc ttggtcttcg gctcgatgcg aagcgacacc acgggctccg
     3901 ggaagacgat ctgctcgagg acgatcgggt tcgcttcgtc gcagagcgtg tgcgaggtct
     3961 tcgtgtcctt catgccgacg aatgcggcga tttctccggt gaacacctgc tcgacttcct
     4021 cgcgcttatc ggcctgcagg cgcacgatgc ggccgacgcg ctccttggtg cccgtgttgg
     4081 cgttgtagat ataggaaccg gcggagaccg tgcccgcata gacgcggaag aaggtcagcg
     4141 cgcccacgaa cgggtcagtc tggagcttga aggcaagcgc cgtgaacggt tcgctgtcgt
     4201 cgggcctgcg ctccacctcc tcgcccgtct tggggttcgt gcccttcacc ggcgggagat
     4261 cgagcggcga cgggaggtag tcgaccacgg cgtcgagcac gagctgcacg cccttgttct
     4321 tcaaggctga gcccgtgtag accgggaaga tctcgttggc gatcaccgcc ttgcggagcg
     4381 tcttcttcag gtcctccaag gatggctcgg tgccctcgaa atacgcggcc atcaaggcat
     4441 catcgtgctc gacgatgcgc tcgatgagct cggcgcggta cttcttcgcc tcctccatat
     4501 actgctccgg gacctcgccc tccacgacgt cctcgcccat attccccgtg aaggtatagg
     4561 acttcatggc caggagatcg atgacgccct cgaagtcact ctcggcgccc atcgggatct
     4621 gcgcgcgcac cgccttcttc gagaggcgat ccagaatgga cttgtaagaa tcctcgaatg
     4681 aggcgcccgt gcggtcgagc ttgttgatga aacagacgcg cggcaccccg gcctcgtccg
     4741 cgtagcgcca gttcgtctcc gactggggct cgacgcccgc gacgccgtcg aagacgacga
     4801 tcgcgccgtc aaggacgcgc atcgagcgct tcacctcggc ggtgaagtcg atgtgccccg
     4861 gggtgtcgat cacgttgaac cgatacttct ttgctgggtc ctttttatct ttctcttcgg
     4921 ttttggtcca gaagcaggtg atggcggcgg cggtgatagt gatgccgcgc tcgcgctcct
     4981 gctccgtcca gtcggtggtg gtctcgccct cgtgcacc
//
LOCUS       DASPKG010000015         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_832474, whole genome shotgun sequence.
ACCESSION   DASPKG010000015 DASPKG010000000
VERSION     DASPKG010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832474"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..939
                     /locus_tag="VEA36_00615"
     CDS             154..939
                     /locus_tag="VEA36_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HYF28852.1"
                     /translation="MAEFRKSYNPNRSADWNYGGHKWRLSRSKIELYNECPRCFYLDN
                     KLGTGRPRGPAFTLNIAVDALLKKEFDVHRANGTKHPICDQYGVDAVPFKHQSIDTWR
                     ENFEGIEYFHEPTGFTISGAIDDLWVNPAGELIVVDYKATAKQGTIASLSDSAWEDSY
                     KRQMGVYQWLLRNNGFTVSPTGYFVYANASSDKEAFDGQLEFEITLVPCTGDTEWIDE
                     VLPKIKQTLDADALPPSGTNCEYCPYREAAGKKLQAIHKAAKR"
     gene            942..1607
                     /locus_tag="VEA36_00620"
     CDS             942..1607
                     /locus_tag="VEA36_00620"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HYF28853.1"
                     /translation="MEVAIEPSWKRKLEEEFGKEYFAELASFVKEEYQTTTIYPHPKN
                     LFKAFELTPFDAVKVVILGQDPYFNPGQAIGLSFAVNEGVRNPPSLQNIFKEVESDMG
                     CAPNPSGDLTRWATQGVLLLNATLTVRGGSAGSHQGKGWETFTDAVIRTLSEEREHLV
                     FILWGNYARSKKALIDWTKHCIIESTHPSPFSANNGFFGSKPFSQANAYLEVNGMEPI
                     DWR"
     gene            1625..2077
                     /locus_tag="VEA36_00625"
     CDS             1625..2077
                     /locus_tag="VEA36_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28854.1"
                     /translation="MTVPSFLRPKRAKFLAFFFLVIYMVASIYWIPLMPYVRYGSLTE
                     SNPTERVLRDGTIAAIVPAYVLLAPFVLIYFSIPNVGGIDVDLGWPSVGRCVPILDEE
                     IKGGITNPSCRTDIALIPFILAILAELAVLYALSCVLVKIPEALRRLY"
     gene            complement(2078..3430)
                     /locus_tag="VEA36_00630"
     CDS             complement(2078..3430)
                     /locus_tag="VEA36_00630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HYF28855.1"
                     /translation="MRPGRRRPSRAGGLRASRSHKTYHKMTVAALAKLAPTIDWKRYL
                     KAQGVDVKQLIVMQPDFLKACAKLLAATPIEDLKTYLEWHLVNDFSGLLSNAFVRQSF
                     SFYGTVLTGTKKMKPLWRRVLGVVNGQVGELLGQIYVKKHFPPDAKRKMDELVDDLFT
                     AYEARIKSLTWMTAPTKKKALQKLRALNRKIGYPDKWKSFKGLVIEPDDYVGNVIRAS
                     EHHHRQEVRKLNKPIDHREWITYPQTVNAFFWPNTNDIIFPAGILQPPFFDFDADDAF
                     NYGCIGMVIGHEITHGFDDEGSKFDAKGNLKSWWTKEDRKRFEKKAAVVRRQFDRYRV
                     EDGVKVNGQLTLGENIADLGGLSIAFDAYQLQLARTGRQTLGGFTPEQRFFLGFAVFE
                     RENIRPEFNKMLALTDTHSPAIFRINGPVSNFDPFYEAYNVTRGDKLYRAPKEREMVW
                     "
     gene            3560..>3730
                     /locus_tag="VEA36_00635"
     CDS             3560..>3730
                     /locus_tag="VEA36_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HYF28856.1"
                     /translation="MGNAPGSRRAGDGPRRVVIAIDGPAASGKSSTAQWVAERLGYRH
                     VDSGSLYRAATAA"
BASE COUNT          708 a         1099 c         1180 g          743 t
ORIGIN      
        1 gaaggccgag aagcggctct taatcgacca aggcatcgtc gcctgcgagc aggtggtgga
       61 gcgccgggac gcgctccggg agctcggcat caccccggag cggatcggca ccatcctggc
      121 ggagacccgg accctctgcg atactcatta ggcatggcag agttcaggaa gagctacaac
      181 ccaaaccgca gtgccgactg gaactacggc gggcataagt ggcggctatc tcgctcgaag
      241 atcgagctct acaacgagtg cccgcgctgc ttctatctgg acaacaagct cgggaccgga
      301 aggccgcgcg gccccgcgtt cacgctcaac atcgcggtgg acgcgctcct gaaaaaggaa
      361 ttcgatgtgc accgcgcaaa cggcactaag caccccatct gcgaccagta cggggtggat
      421 gcggtgccgt ttaagcacca gagcatcgac acctggcggg agaacttcga gggcatcgag
      481 tacttccatg agccgacggg cttcaccatc tcgggcgcca tcgacgacct ctgggtgaat
      541 cccgcaggag agctcatcgt cgtggactac aaggcgaccg ccaagcaggg caccatcgcg
      601 tcgttgtccg attccgcctg ggaggactcc tacaaacgac agatgggcgt gtaccagtgg
      661 ctcctgcgca ataacggctt caccgtatcc ccgaccggct atttcgtcta cgccaacgcg
      721 agctcggaca aggaggcatt cgatgggcag ctggagttcg agatcacgct ggtgccctgc
      781 accggcgata ctgagtggat cgacgaggtg ctgccgaaga tcaagcagac gctggacgcc
      841 gatgcgctcc cgccctcggg cacgaattgc gaatactgcc cgtaccgcga ggcggcggga
      901 aagaagctgc aggccatcca caaggcagcg aagcggtgag tatggaagtg gcgatcgagc
      961 catcatggaa gcggaagctc gaggaggaat tcgggaagga atactttgcc gagctcgcct
     1021 ccttcgtgaa ggaggagtat cagacgacga ccatctatcc gcatccgaag aacctcttca
     1081 aggcgttcga gctgactccc ttcgatgcgg tgaaggtggt catcctcggg caggatccgt
     1141 acttcaatcc tgggcaggcg attgggctct ccttcgcggt gaacgaaggg gtgcgcaatc
     1201 ccccttccct ccagaacatc ttcaaggagg tcgagagcga tatgggatgc gccccgaatc
     1261 cgagcgggga tctcacccgg tgggcaacgc agggcgtgct gctcctgaac gcgactttaa
     1321 cggttcgcgg cggctcggct ggttcgcacc agggaaaggg gtgggagacc ttcacggacg
     1381 cggtcatcag gacgctctcg gaggagcgcg agcatcttgt gttcatactc tggggcaact
     1441 atgcccgcag caagaaagcg ctcatcgatt ggacgaagca ctgcatcatc gagtcgacgc
     1501 atccctcgcc gttctcggcg aataatgggt tcttcgggag caagccgttc agtcaggcga
     1561 acgcgtatct cgaggtgaac gggatggagc cgatcgattg gcgttgaggt gctagcataa
     1621 cgcaatgaca gtaccttcct tcctgaggcc gaagagggcg aaatttctcg ctttcttttt
     1681 cctcgtaatc tatatggtgg ccagtatcta ctggatcccc ctcatgccgt acgtcagata
     1741 cggctcccta accgagagca atcctactga acgagtacta cgggacggta cgatagcagc
     1801 gattgtccca gcatacgtgc tcctcgcccc ctttgtcctg atttacttct ccatccctaa
     1861 tgttggtggc attgacgttg atttgggctg gcctagcgtc ggaaggtgcg ttccaatact
     1921 tgatgaggaa ataaaaggag gcattactaa cccctcgtgc cgtacagata tcgccctcat
     1981 tccatttatc ctcgcgatac tcgccgagct cgcggtgctt tacgcgctca gttgtgtgct
     2041 ggtcaagata cccgaagcac tgaggaggct gtactagcta ccacaccatc tcccgctctt
     2101 tcggcgcgcg atagagcttg tcgccccgcg tgacgttgta ggcctcatag aacgggtcga
     2161 agttcgagac cgggccgttg atacggaata ttgccgggga atgcgtatcg gtgagggcga
     2221 gcatcttgtt gaactcggga cggatgttct cgcgctcgaa gacggcgaac ccgaggaaga
     2281 agcgctgctc gggggtgaag ccgccgagcg tctgccgtcc ggtacgcgcg agctggagct
     2341 ggtacgcgtc gaaggcgatc gagaggccgc cgaggtccgc gatgttctct ccaagggtca
     2401 gctgcccgtt cacctttacg ccgtcttcga cgcgataccg gtcgaactgc ctgcggacga
     2461 ctgccgcctt cttttcgaag cgcttccggt cctctttcgt ccaccagctc ttcaggttgc
     2521 ccttggcatc gaacttggag ccctcgtcat cgaagccgtg agtgatctcg tggccgatga
     2581 ccatgccgat gcagccgtag ttgaaagcgt cgtccgcgtc gaagtcgaag aagggcggct
     2641 gcaggatgcc ggccgggaag atgatgtcgt tggtgttcgg ccagaagaag gcgttcaccg
     2701 tctgggggta ggtgatccac tcgcggtggt cgatgggctt gttgagcttg cgcacctcct
     2761 ggcgatggtg gtgctcgctc gcacggatga cgttgccgac gtagtcgtcg ggctcgatca
     2821 caagcccctt gaaagatttc catttgtcgg ggtacccgat cttccggttg agcgccctca
     2881 gtttctgaag cgccttcttc ttcgtggggg cggtcatcca ggtgagcgat ttgatcctgg
     2941 cttcgtaggc ggtgaagaga tcgtccacga gctcgtccat cttccgcttc gcgtcaggcg
     3001 ggaagtgctt cttcacgtat atctggccga gcaattcgcc cacctgcccg ttcacgaccc
     3061 cgagcacgcg gcgccatagg ggcttcattt tcttcgtgcc cgtgaggacg gtgccgtaga
     3121 aggagaagga ctgcctcacg aacgcgttgg agaggaggcc cgagaaatcg ttcacgagat
     3181 gccactcgag gtaggtcttc aggtcctcga tcggggtcgc ggcgaggagc tttgcgcatg
     3241 ccttgaggaa atccggctgc atgacgatga gctgcttcac gtccacgccc tgcgctttga
     3301 ggtaccgctt ccagtctatg gtcggcgcaa gcttcgcgag cgcggcgact gtcatcttgt
     3361 ggtacgtctt gtgcgatcgc gacgcacgga gaccaccggc tcgcgatggc cttcggcgtc
     3421 ctgggcgcac tgccgggcaa ccggatctcg atcgacgacc cgagctgcgt ggcggtgtcg
     3481 tacccgacct tctggcagga gctcgagcgt gtgcgcggtc acgaaaggtc ggggccgctc
     3541 ccgggggagt ctcgcgccca tgggtaacgc gccggggtcc cggcgcgctg gtgacggccc
     3601 gcgacgcgtc gtcatcgcca tcgacgggcc ggcggcttcc gggaagtcgt cgaccgcgca
     3661 atgggtcgcc gagcggcttg gctaccggca cgtcgattcc gggtccctct atcgggccgc
     3721 gacggccgcg
//
LOCUS       DASPKG010000016        11994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1648562, whole genome shotgun sequence.
ACCESSION   DASPKG010000016 DASPKG010000000
VERSION     DASPKG010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11994)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11994
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648562"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..668)
                     /locus_tag="VEA36_00640"
     CDS             complement(93..668)
                     /locus_tag="VEA36_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28857.1"
                     /translation="MSPERSALEKKEKAMRMIALIILLAALASPGAAQETVPVETTVR
                     PQEIIPANTDIRPQLPEPKFATSAHAYAVTVYEFAGYDAEADPPFILEPVREVETELG
                     AQAYAEFKWNPGYTVVEYCRAVPRPDGLYVLPQTRAERLQGSCMVYGYVAATPRSWHE
                     FLVPVADTVKKIAKKPVKKGPVRRKPPRRRR"
     gene            complement(693..2057)
                     /locus_tag="VEA36_00645"
     CDS             complement(693..2057)
                     /locus_tag="VEA36_00645"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010271553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HYF28858.1"
                     /translation="MKQVSLDIIVSLAKRRGFVYPGSEIYGGLAGTFDYGPHGLTLKK
                     NIERSWWRYFVEGRDDMYGIDSAILMNSKVWEASGHIGGFHDPLVTDLKTKRRYRADH
                     LLEEKGIDPAGMSLEQMGALIKEKGIKSPDGNALSEPQQFNMMFKTSVGSVDGADTPV
                     YLRPETAQGMFVNFKNIIDTFHPRLPFGIAQIGRNFRNEIAPREFLFRVRELEIMEFE
                     YFVAEKDWEEHFEYWLNEMRAWFLSLGFSTEDLKEIEIPEDDRAHYSKRTVDFHFRFP
                     QKFDEIGGIAYRTNYDLKNHIAHSGEDLSYLDPVTNEKFVPHVIEPTFGLGRIVLGVL
                     ANAYDEDELGGEKRVVLRLPAHLAPVKAMVSPLLKNKPELVEKARDVRAELQIVHPAI
                     AWDDNGNIGKRYRRQDEIGTPFCVTVDYDTLADGSVTLRDRDTGEQERLSIEEVQAKL
                     RAAI"
     gene            complement(2063..3928)
                     /locus_tag="VEA36_00650"
     CDS             complement(2063..3928)
                     /locus_tag="VEA36_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28859.1"
                     /translation="MPPEEEKSPLARAQERLYAPTGSTPAAAPALDKKAPPPPAERWT
                     PPPPPPPKKPRLPRTTLFLIGAAVFFTGALIVAAGLILFGGRTVSNENIAIEVTAAPT
                     AGSGDTVDLLITVANENPVPLSGTLLSIDFPEGTRSPENLEAELPQYSDTLGEIPAGG
                     KAERTVRAVIFGAEGQRVSLPIRFEYRTPGASTAFIKEAAHEFTITTSPLSVSIGSLS
                     QVSAGQPFDIAVAVRSNAAAPINNAALVAEYPPGFALRASTPAPALGSLYDLGTIQPG
                     EEKIVRITGTIAGEDRETRVIRFTTGTRAEGSGALSVTYTTAEKPVVVEKPFLAPVLT
                     LNREVGAALAIAPGEEVSGVLSFANTLDAPLEDAKIVVALSGTGLDPTSIRSANGFYR
                     SADNTITFSKETIPALVRLAPGQTGSGSFTFATRPASAGLRNPTVNINISVSGRRINQ
                     PSAPTEISSTVTRTLKVRTDLAAAAQVLRSGPIPTTGPHPPRADTETSYAVKWTFTNS
                     VNAVGGAVVSAVLPSYVRFSGAVSPSDAAITYNEATRTVTWNAGDVPAGASPKSASFG
                     VVITPSASQRGTSPVLIGEASYGASDRFTGERIEGAIRELTNDLSASGGGTGAVQ"
     gene            complement(3955..4506)
                     /locus_tag="VEA36_00655"
     CDS             complement(3955..4506)
                     /locus_tag="VEA36_00655"
                     /inference="COORDINATES: protein motif:HMM:NF023393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HYF28860.1"
                     /translation="MQHKYVTRAFVLGRHPAKEAGASIVLLTAELGLVRARAEGVRRS
                     GAKLAHALQTLSEVDATLLRGKEGWRLSGAVLIEPWFSTLPPAARLRAGRRASLILRL
                     VQGESTDASLFSIYAEFLGALRSEDESLQDSAELRATLQSLSALGHDAGAIPTMEAIR
                     ALSKDERRKLIARINQSLTSTGL"
     gene            complement(4496..7852)
                     /locus_tag="VEA36_00660"
     CDS             complement(4496..7852)
                     /locus_tag="VEA36_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYF28861.1"
                     /translation="MAETGKSEAAKREEEILAFWKERQIFEKSLEKPSPNGEFVFYDG
                     PPFATGLPHSGSLLSSVAKDLIPRYKTMRGYFVRRRWGWDTHGLPIESLVEKKLGLKN
                     KKEILALGIEEFNETARSMVLEYVHDWKRYIDRVGRWVDFDNSYKTMDNTFIESVWWG
                     LKQIYDNGRLYEGRKVLMYCPHCETPLAKAEIAMDNTYKDVTEEATTVKFKIKGEHAY
                     LLAWTTTPWTLPGNVALAVGPDIAYGRYEKDGAEYIVAKERAEALGLANLVSEQQGSA
                     LVGIEYEPLYAIDKVAKVAGKKWQVLAADFVNTEEGTGIVHTAVIYGEDDYQLGVKEG
                     LPMVPLLNPNATYNEDAPEFLRGKYIKKAEADIKEDLESRSLLYAKAPHTHSYPHCYR
                     CGTPLIYNAVSSWFIDIQAVKAKMLAENEKITWVPEHLKQGRVRHILENAPDWTISRN
                     RFWASPLPIWKSEVGEVTVIGSLDELKARTKKSGNRYYVMRHGEAEHNVKGIASCDPT
                     APYSLTENGCKQAGLAAKQLLTAGITKIYASPLVRGRETAQCVASALGFPFEDVVYDD
                     RLREFSFGELDGRPIGEFVAYRAAHGYTDKVPGGESYQDVKNRFGAFLYEREANDADE
                     QILIITHGVGIEAFEALIKGADAAESKRIAEVLDPVCAEVRTLSFVPLPHNRDYELDF
                     HLPYIDQVELVDEQGNTLARIPEVVDCWVESGSMPFAEYHYPFENVEEFKKRAPADFV
                     SEYIGQTRAWFYYLNAVSTQLFERRAFNSVITTGNVLAADGAKLSKSKNNYTDPYHLF
                     DAYGADAFRYHLMASVVMQAEDLVYRDEDVKEAHNRVVGMLRNVASFYQLFADEALEK
                     AALSTHPLDRWILARLRELIEEVTDAFDRYDVPRGTRPVRAFVEDLSTWYVRRSRDRV
                     KGEDAADRVAALATLRQVLKELSKVIAPVMPFIAEDVFQIVREAGDAESVHLCDWPAV
                     SLTIVDRMLGQEKKEQALLEDMAKVRALASEGLMLRQKADVKVRQPLASLSIPGKLPE
                     ELAAILADEVNVHEVKMDAAEVALDTVLTPELVREGDERTFQRAVAEARKAEGFSPKD
                     RATVLRGEDGKYVAELSTGPVRFSLSRNAA"
     gene            complement(7935..8273)
                     /locus_tag="VEA36_00665"
     CDS             complement(7935..8273)
                     /locus_tag="VEA36_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28862.1"
                     /translation="MASGVTATALEFDREELDRRDVSGDGHRWRKKITEACTRHGLSL
                     EELAQNAGHCYFAACRKPRQQKEIQLALRALQDRSWLRRTNGEPHVAHFIRAYASCLL
                     QPPEQMKLAA"
     gene            complement(8366..10045)
                     /gene="argS"
                     /locus_tag="VEA36_00670"
     CDS             complement(8366..10045)
                     /gene="argS"
                     /locus_tag="VEA36_00670"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HYF28863.1"
                     /translation="MEERIRQAVTGALEELGAGEVAFVVEWPAELAHGDFATNAALVA
                     ARELGKSPREIAEALVPLVAEALGDTASKVEAAGPGFVNITLAQPVIAETIAAAADNG
                     TWGSRTDGIGTRVMIEYSNPNPFKEIHIGHLMSNVIGEALSRLMETSGATVLRDTFGG
                     DVGPHVAKALWALRRAGTTDVANAGEIGKAYAQGSTAYEESEAAKQEIDELNTRIYAV
                     VAQAEAPDGFSEDDRALLALWRKGREVSMEEFLRIFKQLGTNFDYIFYDSDTTDTGLR
                     IVREGVAQGIFEESDGAVIYRGEKKGLHTLVFITSRGTPTYETKDIGLAFLKEERAPT
                     DKVYIVTAVEQVGHFNVVLAALSEIAPNLAAKTTHIPHGLLHLTTGKMASRKGNVITA
                     AELITEVIERAAQKNEDLLVAEQVAIGAVKYMILRAAPGSNIVFDPEKSLSLEGDSGP
                     YLQYALVRASSVLASAGEKEVGDAPDAPYSIERLITRFPEVVRRASEECAPQQVAQYL
                     TQLAGEWNSFYAQERIIGGEYEGYKLRLARAFVRTMKNGLTLLGIPTPERM"
     gene            complement(10061..11323)
                     /gene="glyA"
                     /locus_tag="VEA36_00675"
     CDS             complement(10061..11323)
                     /gene="glyA"
                     /locus_tag="VEA36_00675"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HYF28864.1"
                     /translation="MDQYDHLKQEDREIFDAIVGEEERQATGLELIPSENYVSRAVRE
                     AMASTLTNKYSEGYPGKRYYGGQQFTDQVEVIAIDRAKQLFGAGYANVQPLGGANANI
                     AAYMALMEPGDTILGMDLSHGGHLTHGHPVTYISKIFRFVRYKMKDVETGEIDYAALR
                     ETALAEKPKVILAGFSAYPRELEWQRIADIAKEVGACAVADVAHIAGLIAGGAAKNPF
                     DHGFDVLTSTTHKTLRGPRGGLILSKDEGIAKKIDKAVFPGLQGGPHMHQIAAKAVAF
                     REALDPSFKEYAAQVVRNAAAMAEVFKEDGARLIAGGTENHLLLLDAFSSYGISGHDA
                     ETMLDQAGITLNKNSIADDPRKPMDPSGIRFGTPAMTTRGFKEEESKEVARLMIEVLR
                     KRDDATVEKVRARVAELAALHPIPESFV"
     gene            11469..>11994
                     /locus_tag="VEA36_00680"
     CDS             11469..>11994
                     /locus_tag="VEA36_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28865.1"
                     /translation="MTPLEPIRKIGPLFDSLRFKDLKDPCIAFDGNTWHIYGSRGTVI
                     TETWEILHATAPTIDGPWTEAVESCLLRGVHGPHVAAPSVYYDPADKLFHMAVQKDFM
                     DVGGDIAYLVSADGHIFTKMRTLVKPKGSVEAGLYDPHFAEVNGKKYLVYSGVPASES
                     EDRPFIPQPDVYLAK"
BASE COUNT         1968 a         3904 c         3801 g         2321 t
ORIGIN      
        1 tggtgacgtt cttatccatg gcgtaaatgc tagcacagac tagataattc ctcgctaaaa
       61 agcaaagggc cgctcctgag cgggagcggg ccctagcgcc tgcgacgagg cggcttgcgc
      121 ctcaccggcc ctttcttcac cggcttcttg gcaatcttct tgaccgtgtc ggcgaccggg
      181 acgaggaact cgtgccaaga ccgcggggtc gccgcgacgt acccgtagac catgcaggag
      241 ccctggagcc gttctgcgcg cgtctgcgga aggacgtaga ggccgtccgg gcgagggacc
      301 gcccggcaat actcgacgac ggtatagccc gggttccact tgaactcggc gtacgcctgt
      361 gcaccgagct ccgtctccac ctcccgcacc ggctcgagga tgaacggcgg atccgcttcg
      421 gcatcgtagc cggcgaattc gtagacggtc actgcgtacg cgtgcgcgct cgtcgcgaac
      481 ttgggttccg gcagctgcgg acggatatcc gtattcgccg gaatgatctc ctgcggacgg
      541 acggtcgttt caaccggaac ggtttcctgt gcggcgcccg gggaagcgag ggccgcgagc
      601 aggatgatca gggcgatcat cctcatggcc ttctccttct tctccagagc ggaacgctct
      661 ggagacatcc tgcacctaac cggcttcccg cgtcaaatag cagcgcgcag cttcgcctgt
      721 acctcctcga tggagagccg ctcctgctcc cccgtatccc ggtcccggag cgtcacgctg
      781 ccgtccgcga gcgtatcgta atcaacggtg acgcagaacg gcgtcccgat ctcgtcctgc
      841 ctccggtagc gcttcccgat attcccgtta tcgtcccagg cgatcgctgg gtgcactatc
      901 tggagttcgg cgcgcacatc cctcgccttc tcgacgagct cgggcttatt cttcaggagc
      961 ggtgagacca tggctttcac gggagcgagg tgcgccggga gccggagcac cacgcgcttc
     1021 tcgccgccga gttcgtcctc gtcataggcg ttcgcgagca caccgagcac gatgcggccg
     1081 agcccgaagg tcggctcgat gacgtgtggt acgaacttct cgttcgtcac cgggtcgagg
     1141 tacgagaggt cttccccgct atgcgcgatg tggttcttga ggtcgtaatt agtgcggtag
     1201 gcgattcccc cgatctcatc gaacttctgc gggaagcgga aatggaagtc caccgtacgc
     1261 ttggagtaat gtgcgcggtc atcctcaggg atctcgatct ccttgaggtc ctccgtggag
     1321 aacccgagcg agaggaacca agcccgcatt tcgttgagcc agtactcaaa atgctcctcc
     1381 cagtccttct cggccacgaa gtactcaaac tccatgatct cgagctcgcg gacccggaac
     1441 aggaactcgc gcggcgcgat ctcgttgcgg aagttgcgcc cgatctgcgc gatgccgaac
     1501 gggagcctcg ggtggaaggt atcgatgatg ttcttgaagt tcacgaacat gccctgcgcg
     1561 gtctcgggac gtaggtacac cggcgtgtcc gcgccatcaa cggagcccac cgaggtcttg
     1621 aacatcatgt tgaattgctg cggctccgag agcgcgttgc cgtccgggct tttgatgccc
     1681 ttctctttga tgagtgcgcc catttgctcg agcgacatcc cggccgggtc gatacccttc
     1741 tcctcgagga ggtggtcggc gcggtaccgg cgcttcgtct tcaggtccgt cactaaggga
     1801 tcatgaaatc ccccgatatg ccccgaagcc tcccagacct tcgagttcat gaggatggcc
     1861 gagtcgatgc cgtacatgtc gtcgcggcct tctacgaagt accgccacca ggatcgctcg
     1921 atgttcttct tgagcgtaag cccatgtggg ccgtagtcga aggtcccggc aagcccgccg
     1981 tagatctcgg agccgggata tacgaaccca cggcgcttgg cgagcgagac gataatgtcg
     2041 agactaacct gcttcatacg gcctactgta ctgcacccgt gccccctccg gaagcgctga
     2101 ggtcgttcgt gagctcccgg atggccccct cgatgcgctc gcccgtgaag cgatcgcttg
     2161 cgccatagct tgcctccccg atgaggacag ggctcgtgcc gcgctgcgac gcgctcgggg
     2221 tgatgaccac gccgaaggat gccgacttcg gcgaagcccc tgcgggaacg tcgcccgcgt
     2281 tccaggtgac ggtgcgcgtc gcctcgttgt aggtgatggc ggcgtcagac gggctcacgg
     2341 cgcccgagaa ccgcacgtag gacgggagga ccgcggagac gaccgccccg ccgaccgcgt
     2401 tcaccgagtt cgtgaaggtc cacttcaccg cgtacgaggt ctcggtgtcg gcacgcggcg
     2461 gatgcgggcc tgtcgtcggg atggggccgc tgcggagcac ctgggccgcc gcggcaagat
     2521 cggtccggac tttgagggtg cgcgtcaccg tcgagctgat ctcggtgggc gcgctcggct
     2581 ggttgatgcg gcgtccggag accgagatgt ttatgttcac cgtcgggttg cgcagccccg
     2641 cggatgccgg ccgcgtcgcg aaggtgaagg acccgctccc ggtctgcccg ggcgcaaggc
     2701 gcacgagcgc cgggatggtc tccttcgaga aggtaatggt gttgtccgcc gagcggtaga
     2761 agccgttggc gctccggatg ctcgtcggat cgagcccggt gcccgagagc gcgacgacga
     2821 tcttcgcgtc ctcgagcggc gcatcgagcg tattggcgaa ggagagcacg cccgacacct
     2881 cctccccggg cgcgatcgcg agcgccgccc cgacctcgcg gttcagggtc aggaccggcg
     2941 cgaggaaggg cttctcgacc acgaccggct tctcggcggt ggtgtaggtt accgaaagcg
     3001 cgcccgatcc ttcggcacgg gtgcccgtcg tgaaccggat gacgcgggtc tcgcggtcct
     3061 cgccggcgat cgtgcccgtg atgcgcacga tcttctcttc cccgggttgg atggtgccga
     3121 gatcgtagag cgagccgagc gccggcgcgg gcgtggaggc gcgcagcgca aagccgggcg
     3181 ggtactcggc gacgagcgcg gcgttgttga tgggtgctgc ggcgttcgag cgcacggcga
     3241 ccgcgatatc gaagggctgg cctgcggata cctgggagag cgagccgatg gagacagaca
     3301 ggggcgacgt cgtgatggtg aactcgtgtg ccgcctcctt gatgaacgcg gtgcttgccc
     3361 ccggcgtccg gtactcgaag cggatcggga gcgagacccg ctggccctcc gccccgaaga
     3421 tgaccgcgcg cacggtgcgc tcggccttcc cgcccgccgg gatctcgccg agcgtatcgc
     3481 tgtactgcgg gagttccgcc tcgaggttct ccggggagcg cgtcccctca gggaaatcga
     3541 tggaaaggag cgtgcctgag agcggcacgg ggttttcgtt cgcgaccgtg atgaggagat
     3601 cgacggtgtc cccgctgcct gcggtcggcg cggcggtcac ctcgatcgcg atgttctcgt
     3661 tcgaaacggt gcggccgcca aagaggatga gtccggctgc gacgatgagc gcgcccgtga
     3721 agaagaccgc cgccccgata aggaagagcg tggtgcgcgg gagccgcggc ttcttcggcg
     3781 ggggtggagg tggcggggtc cagcgctcgg cgggaggcgg cggcgctttc ttatcgagcg
     3841 cgggagccgc ggcgggcgta ctgcccgtcg gtgcgtagag gcgttcctgc gccctcgcga
     3901 gcgggctttt ctcttcctcg ggaggcatat ccttcaaagt atagcgtaca cagtttatag
     3961 acccgtggat gtgaggcttt gattgatgcg tgcgatgagc ttccttcgct cgtccttcga
     4021 gagcgcccgt atcgcttcca tggtcgggat ggcccctgcg tcgtggccga gggcggaaag
     4081 cgattgcaga gtcgctcgca gctccgcgct gtcctgcagg gactcgtctt cgctgcggag
     4141 cgcaccgagg aattcggcgt agatggagaa gagggatgca tcggtgctct cgccctgcac
     4201 gaggcggagg atgaggcttg cgcggcgccc cgcccggagc cgggcagccg gggggagtgt
     4261 cgagaaccag ggctcgatga ggaccgctcc cgagagccgc cacccctcct tgccgcgcag
     4321 cagggtcgcg tccacctccg agagcgtctg gagcgcgtgc gcgagtttgg cgcccgagcg
     4381 gcgcacccct tcggcgcgtg cgcggacgag cccgagttcc gcggtgagca ggacgatgct
     4441 cgcacccgct tccttggccg gatgccggcc gagcacgaac gcccgcgtga cgtacttatg
     4501 ctgcattgcg cgagagcgag aagcgcacgg ggccggtcga gagctccgcg acgtacttcc
     4561 cgtcctcgcc gcgaaggacc gtcgccctat ccttcgggga gaatccctcc gccttgcgcg
     4621 cttcggcgac cgctcgctgg aaggtgcgct cgtccccttc ccgcacgagc tcgggggtga
     4681 gtaccgtatc gagcgcgact tctgccgcgt ccatcttcac ctcatgcacg ttcacctcat
     4741 ccgcgaggat cgcggcgagt tcctcgggga gcttcccggg tatggagagt gaggcgagcg
     4801 gctggcgcac cttcacgtcc gccttctgcc gaagcatgag gccttcggag gcgagcgccc
     4861 ggaccttcgc catgtcctcg aggagcgcct gctccttctt ctcctgcccg agcatccggt
     4921 ccacgatggt gagcgagacg gcgggccaat cgcagaggtg cacgctctcg gcatcacccg
     4981 cctcccgcac gatctggaac acgtcctcgg caatgaacgg catcacgggc gcgatgacct
     5041 tcgaaagctc cttgagcacc tgccgcaggg tcgcaagcgc cgcaacgcga tccgcggcgt
     5101 cctcgccctt cacgcggtcg cgcgaacgcc tcacgtacca cgtcgagagg tcctcgacga
     5161 aggcgcggac gggacgggtg ccgcgcggca cgtcgtagcg atcgaaggcg tcggtcactt
     5221 cctcgatgag ctcgcggaga cgcgcgagga tccagcgatc caaggggtgc gtgctgagtg
     5281 cggccttttc gagcgcctcg tccgcgaaga gctggtagaa gctcgcgacg ttccgcagca
     5341 tccccaccac ccggttatgc gcctccttca cgtcctcgtc ccgatagacg aggtcctcgg
     5401 cctgcatcac cacgcttgcc atgaggtggt agcggaaggc gtcggcgccg tacgcgtcga
     5461 agaggtgata gggatcggtg tagttgttct tactcttgga gagcttcgcg ccgtctgcgg
     5521 cgagcacgtt ccccgtcgtg atgacgctgt tgaaggcgcg gcgctcgaag agctgcgtgc
     5581 tgactgcatt caaataatag aaccaggcgc gggtctgccc gatgtactct gagacgaagt
     5641 cggcgggcgc gcgcttcttg aattcctcga cgttctcgaa gggatagtgg tactccgcaa
     5701 acggcatgga ccccgactcc acccagcagt cgacgacctc cgggatgcgc gcgagggtat
     5761 tgccctgctc gtcgacgagc tcgacctggt cgatgtacgg gagatggaag tcgagctcgt
     5821 agtcgcggtt gtgcgggagc gggacgaagg agagcgtgcg cacctcggcg cagaccggat
     5881 cgaggacctc cgcgatgcgc ttcgattccg ctgcgtccgc gcccttgatg agcgcttcga
     5941 acgcttcgat gccgacccca tgcgtgatga tcaggatctg ctcgtccgcg tcgttggcct
     6001 cgcgctcgta gaggaacgcg ccgaaccgat tcttcacgtc ctggtaactc tcgccgccgg
     6061 gcaccttatc cgtatacccg tgcgccgctc ggtaggcaac gaattcgccg atcggccggc
     6121 catcgagctc gccgaacgag aattcgcgga gccggtcatc gtatacgacg tcctcaaagg
     6181 gaaagccgag cgcgctggca acgcactgcg ccgtctcgcg accacgcacc aagggcgatg
     6241 cgtagatctt cgtgatgccc gcggtcagga gctgcttggc ggcgagcccc gcctgcttgc
     6301 agccgttttc cgtgagcgaa tacggcgcgg tcgggtcgca gctcgcaatg cccttcacgt
     6361 tgtgctccgc ctccccgtgc cgcatcacgt agtagcggtt gccgctcttc ttcgtccgtg
     6421 ccttgagctc atccaacgac ccgatcaccg tcacctcccc gacctcgctc ttccatatag
     6481 gaagcgggct cgcccagaag cgattgcggg agatggtcca atctggcgcg ttctcgagga
     6541 tgtgccgcac ccggccctgc ttcaggtgct ccggcaccca ggtgatcttc tcgttctcgg
     6601 caagcatctt cgccttcacc gcctggatgt cgatgaacca ggacgagacc gcgttataga
     6661 tgagcggggt gccgcagcgg tagcagtgcg ggtacgagtg cgtatgaggg gccttggcgt
     6721 agaggaggct gcggctctcg agatcctcct tgatgtccgc ctccgccttc ttgatgtact
     6781 tgccgcgaag gaattccggc gcgtcctcgt tatacgtcgc gttcgggttc aggagcggga
     6841 ccatggggag cccctccttc acgccgagct ggtagtcgtc ctcgccgtag atgacggcgg
     6901 tgtgcacgat gccggtcccc tcctcggtat tcacgaagtc cgcagcgagg acctgccact
     6961 tcttcccagc gaccttcgcg accttatcga tcgcgtacag cggctcgtac tcgatcccga
     7021 cgagtgcgct cccctgttgc tcgctcacga ggttcgcaag gccgagcgcc tcggcgcgct
     7081 ccttcgctac gatgtactcc gctccatctt tctcgtaccg gccgtaggca atgtcggggc
     7141 ccaccgcaag cgcgacgttc ccgggcaggg tccagggcgt cgtggtccag gcgaggagat
     7201 atgcatgctc gcccttgatc ttgaacttca cggtcgtcgc ctcctcggtc acgtccttgt
     7261 acgtgttatc catcgcgatc tccgccttgg cgagcggggt ctcgcagtgc gggcagtaca
     7321 tgaggacttt ccgcccttcg tacaagcggc cattgtcgta gatctgcttc aggccccacc
     7381 agaccgattc gatgaacgta ttgtccatcg tcttgtacga gttatcgaaa tccacccagc
     7441 gcccgacgcg atcgatgtag cgcttccagt catgcacgta ctcgaggacc atggagcgcg
     7501 ccgtctcgtt gaactcctcg atgccgagcg cgaggatctc tttcttgttc ttgaggccga
     7561 gcttcttctc caccaaggac tcgatgggga gcccgtgggt gtcccagccc cagcggcgac
     7621 ggacgaaata cccgcgcatg gtcttgtacc gcgggatgag gtccttggcg acggacgaga
     7681 ggagggatcc cgaatgcggg agcccggtcg caaacggggg gccgtcgtag aacacgaatt
     7741 cgccgttcgg cgaaggcttt tcgagcgatt tctcgaagat ctgccgctcc ttccagaacg
     7801 cgaggatctc ctcctcgcgc ttggcagctt cgctcttgcc cgtttcagcc atgattttcc
     7861 tatcgtatag cctgcttcat aaaaagggaa cggcctggcg cgcggagcgc caggcctgat
     7921 tccgcggttc gtccttaggc ggccagcttc atctgctcgg gtggctggag caggcaggac
     7981 gcgtaggcgc ggatgaagtg agcgacatgg ggctcaccgt tggtgcgccg gagccagctc
     8041 cggtcctgca gcgcgcggag cgcgagctgg atctccttct gctgccgcgg ctttcggcac
     8101 gcggcgaagt agcagtgccc ggcgttctgc gcgagctctt cgagcgagag gccgtgacgg
     8161 gtacaggcct cggtgatctt cttcctccac ctgtggccat cgccactgac atcccgacgg
     8221 tccagctctt cgcgatcgaa ctcgagagcc gttgctgtaa cacctgacgc catcctcgct
     8281 attcccccaa gtatggtgac gactcgcacg aaaggtacgg gcctgcctcg caccttaacg
     8341 gttcaagctc gtccgtgcaa gtgaattaca tccgctccgg cgtcggtata ccaagcagcg
     8401 taagcccgtt cttcatggtc cgcacgaacg cacgtgcaag ccggagcttg tatccctcgt
     8461 actccccgcc gatgatccgc tcctgcgcat aaaaagaatt ccactcgcca gcgagctgcg
     8521 tgagatactg cgcaacctgc tgcggcgcgc actcctcgct cgcccggcgc accacttccg
     8581 ggaaccgggt gatgagccgt tcgatgctgt acggggcatc gggagcatca cccacttcct
     8641 tttcccctgc gcttgcaagc accgacgacg cgcggaccag cgcgtactgc aggtacggcc
     8701 ccgaatctcc ttcgagggag agcgacttct cgggatcaaa cacgatgttg gacccgggcg
     8761 ccgcacgaag gatcatgtac ttcaccgcgc cgatcgccac ctgctcggcg accaaaagat
     8821 cctcgttctt ctgagccgcc cgctcaatca cctcggtgat caattcggct gccgtgatca
     8881 cattcccctt gcgcgatgcc atcttgccgg tcgtcaaatg gaggagtccg tgcggtatgt
     8941 gcgtggtctt cgctgcaagg ttcggtgcga tctcggaaag tgccgccagc accacattga
     9001 agtgccctac ctgctcgacc gcggtcacga tatacacctt atccgttggc gcgcgctcct
     9061 ccttcaggaa ggcgaggccg atgtccttgg tctcgtaggt cggcgtgcct cgggacgtga
     9121 tgaacacgag cgtatgcaga cccttcttct cgccgcgata gatgaccgcg ccatcgctct
     9181 cttcgaagat gccttgagcg accccctccc gcacgatgcg caatccggtg tcggtcgtat
     9241 cgctatcgta gaagatgtag tcgaaattgg tcccgagctg cttaaagatg cgcaggaact
     9301 cctccatgga gacttccctg cccttgcgcc agagcgcgag aagcgcgcga tcgtcttccg
     9361 aaaacccgtc cggtgcctcg gcctgcgcga cgactgcgta gatgcgcgtg ttgagctcat
     9421 cgatctcctg cttagccgct tcgctttcct cataagcggt cgacccctgc gcataggcct
     9481 tgccgatctc gccggcgttc gcgacatcag tcgtacctgc gcgccggagt gcccagagag
     9541 ccttggccac gtgcgggccg acatctccgc cgaatgtatc gcgcagcacg gtcgctccgg
     9601 aggtttccat gagccgcgag agtgcctcac cgatcacgtt actcatcagg tgaccgatgt
     9661 gtatctcctt gaaggggttc gggttcgaat actcgatcat cacccgcgtg ccgataccat
     9721 ccgttctgct gccccacgtt ccattgtcag ctgcagctgc aatggtctcg gcaatgacgg
     9781 gttgcgccag cgtgatgttc acgaagccgg gacctgccgc ttcgaccttt gacgccgtat
     9841 caccgagcgc ctcggcgacg agcggcacga gcgcctccgc gatctcccgc gggctctttc
     9901 ctaattcgcg ggccgccacg agcgctgcgt tggtcgcgaa gtcgccatgg gcaagctctg
     9961 ccggccactc gacgacaaac gcgacctcgc ctgcgccgag ctcttcgagc gcccccgtta
    10021 ccgcctgcct gatccgttct tccatggggc aactctagca ctagacgaag gactcgggga
    10081 tggggtgtag agcggcgagc tccgcgaccc gcgcacgcac cttctcgaca gtcgcatcgt
    10141 cgcgcttccg gaggacctcg atcatgaggc gcgccacctc tttcgattcc tcctccttga
    10201 acccgcgggt cgtcatcgca ggcgtgccga accggatgcc cgagggatcc atgggcttcc
    10261 tagggtcgtc cgcgatcgag ttcttgttga gcgtgatgcc cgcctgatcg agcatggttt
    10321 ccgcgtcatg cccggagatg ccgtacgagg agaatgcatc gaggaggagg agatggttct
    10381 cggtgccgcc cgcgatgagt cgcgcgccgt cttccttgaa cacctcggcc atcgcggcgg
    10441 cgttgcgtac cacctgtgct gcgtattcct tgaaggacgg gtcgagcgcc tcgcggaacg
    10501 cgaccgcctt cgctgcgatc tggtgcatgt gcggaccgcc ctggagccct gggaagaccg
    10561 ccttgtctat cttcttcgcg atgccctcgt ccttgctgag gatcaagcca ccgcgcggcc
    10621 cgcgcagcgt cttatgcgtc gtcgaggtca gcacatcgaa gccgtgatcg aagggattct
    10681 tcgccgcccc tccggcaatg agccccgcga tgtgcgcgac gtccgcgacg gcgcatgccc
    10741 cgacttcctt ggcgatgtct gcgatgcgct gccactcgag ctcgcgcgga tacgccgaga
    10801 accccgcgag gatgaccttc ggtttctcgg cgagcgccgt ctcgcggagc gccgcgtaat
    10861 cgatctcgcc cgtctcaacg tccttcatct tgtagcggac gaagcggaag atcttcgaga
    10921 tgtaggtcac ggggtgcccg tgcgtgagat gtccgccatg cgagaggtcc atgccgagga
    10981 tggtgtcgcc gggctccatg agcgccatgt atgcggcgat gttggcgttc gcgcccccca
    11041 agggctgcac attcgcgtat cctgcaccga ataattgctt cgcgcggtcg atggcgatca
    11101 cttccacctg atcggtgaac tgctgcccgc cgtagtaacg cttgccgggg taaccctccg
    11161 aatacttgtt cgtgagggtc gaggccatcg cctcgcgcac cgcgcgtgag acgtagttct
    11221 ccgacgggat gagctcgagg cccgtcgctt ggcgctcctc ttcgcccacg atggcgtcga
    11281 atatctcccg gtcttcttgc ttgaggtggt cgtactggtc catggcgtaa tcgcttagat
    11341 gaataacggg aagccggtgg accgtcagac cataggaatc gtagccccca tgataccgca
    11401 cccgaagagg cgtacaatga cacccattca tggcgcattc agagccgagg ccctaccctt
    11461 tcgcggatat gacgccgctt gagccgatca ggaagatcgg ccccctattc gattcgctcc
    11521 ggttcaagga cctcaaggac ccctgcatcg ccttcgatgg gaatacctgg cacatctacg
    11581 gctcccgcgg caccgtcatc accgagacat gggagatcct gcatgcgacc gcgcccacca
    11641 tcgacgggcc ctggaccgaa gcggtcgagt cctgcctgct ccgcggcgtc cacggtccgc
    11701 atgtcgcggc gccctccgtc tactacgacc cggcggacaa gctcttccac atggcggtgc
    11761 agaaagactt catggacgtg ggcggcgata tcgcctacct cgtctccgcc gatggccaca
    11821 tcttcacgaa gatgcgcacc ctcgtgaagc ccaagggctc ggtcgaggcc ggcctctacg
    11881 atccgcactt cgccgaggtg aacgggaaga agtacctggt gtattccggc gtcccggcgt
    11941 ctgaatcgga agaccgcccc ttcatccccc agccggatgt gtatctcgcg aaga
//
LOCUS       DASPKG010000017         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1513309, whole genome shotgun sequence.
ACCESSION   DASPKG010000017 DASPKG010000000
VERSION     DASPKG010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513309"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2622)
                     /locus_tag="VEA36_00685"
     CDS             complement(<1..2622)
                     /locus_tag="VEA36_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HYF28866.1"
                     /translation="MIHEIRVSDTGSDPKGAELLAEMKRLGLGCFTAIRTTRVYRLQG
                     TSEAEAKRFAEAITDVDQTHTLNQEPAPDGAAVSEVAYKPGVMNPVSASLKKIAHDLG
                     ITLEAADSSIEYRFYPGENRDAVREVLDRPELVNKTVQRVVTKKPDTLLIEDTPGSIE
                     IVRIRDLSEDELIQLSRERELHLNLAEMLLLQDFARKEGRELTDAEVEMVAQYWSEHC
                     KHKTFAALLETPQGIKRPLMERLKEASALYQRGVISAFGDNSGVVWFFDDWAINAKVE
                     THNSPSAIEPYGGAATGVGGVLRDINGTGKGAKPILSTDMFCFAPPSLDPSDLPKGCL
                     PPDYLLRQVVAGVRDYGNRMGVPTANGSIHFHPDFRAKPTVIVGAYGILPTKYAEKGK
                     PQEGDLVLLVGGRTGRDGIHGATFSSGEMTEMTAIRNGTAVQIGNAIEEKRMFEAILL
                     LRDQGIIRAITDCGAGGLSSAVGEMGERTGVHLWAEEVPLKYSGLAPREVVLSESQER
                     MVLAVEHEDAEEAIALCRSLNVEATVVGKFTDTERFVVTYKGETVADLPYAFLKGLPQ
                     RTMPADWQRPVFDEPAEGQFASLDLSRAFMKVLGDLNVCSKEPIVRMYDHSVQGTSAL
                     PPFTGVHHDGPSDACVLRPVLGQPWGLVVSHGMDPILNRIDPYWGSVCAAVEALANLV
                     AVGGNPVQGEAWLIDNFIWPFPDAESLGALDLSMDGLIAVMNAFGIPCVSGKDSLSST
                     YRSGNTVIKIPPVLCVSAFGRIPNVRRTVSADFKRPDSRIVFVGLPDWGAMGGSVLYD
                     KLGYVVNRGPTYADGRVYYTTLDAHVVAVDAETGKPAWNVKVGDIQIGETMTMAPLVA
                     KGKVIVGNSGGEYGVRGW"
BASE COUNT          508 a          937 c          901 g          571 t
ORIGIN      
        1 ccagccgcgg acgccgtact cgccgccgga attgccgacg atcaccttcc ccttcgcgac
       61 cagcggcgcc atcgtcatgg tctcgccgat ctggatgtct ccgaccttca cgttccaggc
      121 cggcttgcca gtctccgcat cgaccgcaac cacatgagca tcgagggtgg tgtagtagac
      181 gcgcccgtcg gcataggttg gtccgcggtt gacgacgtag cccagcttgt cgtagagcac
      241 cgaaccgccc atcgcccccc aatcgggcag gccgacgaat acgatccggg agtcgggccg
      301 cttgaagtct gccgacaccg tgcgcctgac gttcgggatt cttccgaacg cggagacgca
      361 gagcactggc gggatcttga tgacggtgtt gccgctccgg taggtgctcg acagcgagtc
      421 cttgccggag acgcacggga tgccgaacgc gttcatgacg gcgatgaggc cgtccatgga
      481 caggtcaagc gctccgagcg attcggcgtc gggaaacggc cagatgaagt tgtcgatgag
      541 ccaggcttcg ccttggacgg gattaccgcc cacggcgacg aggttggcca gcgcttcaac
      601 ggccgcacac accgagcccc agtacggatc gatccggttg aggatcgggt ccatgccgtg
      661 gctcaccacg agtccccagg gctgcccgag gacaggccgg agtacgcacg cgtccgaggg
      721 gccgtcgtga tgcacgcccg tgaagggcgg cagagcactc gtgccctgga cgctatggtc
      781 gtacatgcgt acgatcggct ctttggagca gacattcagg tctccgagga ccttcatgaa
      841 cgcccgggag agatcgagcg aggcgaactg accttcagca ggctcgtcga agacgggccg
      901 ctgccaatca gccggcatcg tcctctgcgg caggccctta aggaaagcgt agggcaagtc
      961 ggccaccgtc tctcccttgt acgtcacgac gaagcgctcg gtgtcggtaa acttcccgac
     1021 cacggtcgcc tcgacgttga ggctgcggca gagcgcgatc gcctcttccg catcttcgtg
     1081 ttcgacagcg agcaccatcc gctcctgcga ctccgagagg acgacctccc gaggcgcaag
     1141 cccggaatac ttgagcggca cttcctccgc ccagaggtgg acgccggttc gttcgcccat
     1201 ctcgccgacg gcggaggaga gacctcccgc cccgcagtcg gtgatagcgc ggatgatgcc
     1261 ctgatcccgc agaagcagga tcgcttcgaa catccgcttc tcctcgatgg cgttgccgat
     1321 ctgcacggca gtcccgttgc ggattgccgt catctcggtc atctccccgg aggagaacgt
     1381 cgccccatgg atgccatcgc gtcccgttcg accgccgacg aggagcacga gatcgccttc
     1441 ttgaggcttt cccttctccg cgtatttggt cggcaggatg ccgtatgcac ccacgatgac
     1501 ggtgggcttc gcccggaaat ccgggtggaa gtgaatcgac ccgttagcgg tcggcactcc
     1561 catccggttg ccgtagtcac gaacgccggc gaccacctgc cgaagcaggt agtcgggcgg
     1621 caggcagccc ttcggaagat ccgagggatc gagcgaagga ggcgcgaagc agaacatgtc
     1681 ggtcgagagg atgggcttgg cgcccttccc cgtgccattg atgtcccgca gtacgccgcc
     1741 gacgccggtc gcggctcccc cgtacggctc gatagcgctc gggctgttgt gggtctcgac
     1801 cttggcgttg atagcccagt cgtcgaagaa ccagacgacg ccggagttgt cgccgaacgc
     1861 ggagataacc ccgcgctggt acaaagccga cgcttccttc aggcgctcca tgagggggcg
     1921 cttgattcct tgcggcgtct cgaggagcgc cgcaaacgtc ttatgcttgc agtgttcgct
     1981 ccaatactgg gcgaccatct ccacctcggc gtcggtgagc tcgcggccct ccttcctggc
     2041 gaaatcctgc agaagcagca tctcggccag gttgaggtga agttcgcgct ccctggagag
     2101 ctggatcagc tcatcttcgg agaggtcgcg gatgcggacg atctcgatcg acccgggcgt
     2161 atcctcgatg aggagagtat cgggcttctt cgtgaccacc cgctggaccg tcttgttgac
     2221 gagctctggc cggtctagga cttctcgtac cgcatcccga ttctcgccgg gatagaagcg
     2281 atactcaatg ctcgagtcag ccgcctccag tgtgatgccg agatcgtgcg cgatcttctt
     2341 gagcgacgca ctcaccggat tcatcacgcc gggcttgtag gcgacttcac tgacggcggc
     2401 tccgtccgga gcgggttcct gattgagggt atgagtctga tcgacgtccg tgatcgcctc
     2461 ggcgaaccgc ttcgcctcgg cttcactggt gccctgcaat cgatagaccc gggtcgtacg
     2521 gatagccgta aagcatccga gcccgagccg cttcatttca gcaagaagtt cggccccctt
     2581 agggtcgctt cccgtgtcgg agacgcggat ttcgtggatc atcctaccct tcccttcctt
     2641 cgtctattgt ctgtggtcct ggcaaggtgc ttgccggaaa accggctacc cccgcaccat
     2701 tgcagggatt cctggcagac aggctaacac ttctcttctg tgactgcacg caggcagttt
     2761 tccgcctatt cctccgcctt ttcgatagaa gtcgccatga ttatgccgtc gatgtcgtat
     2821 ttctgtacgc tgctcaggtg cgtcgcgggc gtcatgacgc cctctacctt caccttggtg
     2881 cccgtaggga aatctaccgg ctgggaggac atgagag
//
LOCUS       DASPKG010000018         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_973381, whole genome shotgun sequence.
ACCESSION   DASPKG010000018 DASPKG010000000
VERSION     DASPKG010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973381"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /gene="rpsG"
                     /locus_tag="VEA36_00690"
     CDS             <1..409
                     /gene="rpsG"
                     /locus_tag="VEA36_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HYF28867.1"
                     /translation="EAISRFINTVMWDGKKDTARKVVYAALDKLKEKEMDPLETFETA
                     IRNVSPLMEVRSRRVGGANYQVPREVPQQRRISLAYRWLINAARAKKGKPMADKLAEQ
                     IILAAKNEGDAIKKKEDMHRMAEANKAFAHFAW"
     gene            469..735
                     /locus_tag="VEA36_00695"
     CDS             469..735
                     /locus_tag="VEA36_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28868.1"
                     /translation="MDGNVVFDSERDPTLMRRSSGQKAGVPLGELITPGPQKDKKQTD
                     KVVLGILGLVLMGSVYVAINTRPKEIVVLTPADAPVINPAIIKP"
     gene            737..1198
                     /locus_tag="VEA36_00700"
     CDS             737..1198
                     /locus_tag="VEA36_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HYF28869.1"
                     /translation="MASARGFTLIELLVVVAIIGILASVTLASLNVARNKAQDARRKA
                     DVGQLVRAINNYYTSTGSLPQGSGWCSFISNTDSGRDVAFRNDMVPAYLPKVPRDPVH
                     GGIPGDYFYANQNDNRGQFTVCATLANDPGTSEPNLFGCAGWGSYNHCVSQ"
     gene            1264..2079
                     /locus_tag="VEA36_00705"
     CDS             1264..2079
                     /locus_tag="VEA36_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HYF28870.1"
                     /translation="MHPFAHELTSRERAVIESGTTYRVATASAVLYHEPTDDSQPITT
                     VLHGEHFYVQSENEDGWAYGYASASGWMGHLRISELHRCERIPTHRICQLFTLVYAQP
                     THRSRVLMQLGMNARVHVTDATRKFYQVATGGWIPKTHLEIINRVSPDFVAEAEKLVG
                     IPYRPGGRDPLLGLNCSGLVHHALLTRNIILSHGVRHMERELEKVAIDVYPHGPFKRG
                     DLVFWDRHVGIMVDDARLVHATNIAMRTLIQPLDEVNPRQGPGGITSVWRLAA"
     gene            2209..>2453
                     /locus_tag="VEA36_00710"
     CDS             2209..>2453
                     /locus_tag="VEA36_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009603844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYF28871.1"
                     /translation="MQRDYPLEKVRNFGIVAHVDAGKTTTSERVLFYTGSQHKIGEVH
                     TGETTTDWMEQERERGITITSAAITCFWSKTQEKDKK"
BASE COUNT          488 a          800 c          735 g          430 t
ORIGIN      
        1 cgaggccatt tcgcgcttca tcaacaccgt catgtgggac gggaagaagg acaccgcccg
       61 caaggttgtg tacgccgcgc tcgacaagct gaaggagaag gagatggatc ccctggagac
      121 cttcgagacc gcgatccgca acgtctcccc cttgatggag gtccgctccc gccgcgtcgg
      181 cggcgccaac taccaggtgc cccgcgaggt gccccagcag cgccgtatct cccttgcgta
      241 ccgctggctt atcaacgctg cgcgcgccaa gaagggcaag cctatggcag acaagctcgc
      301 cgagcagatc atcctcgcgg ccaagaacga gggcgacgcg atcaagaaga aggaggacat
      361 gcaccgcatg gcggaagcca acaaggcctt cgcacacttc gcctggtaat tttcacgagc
      421 atccaaaagc gccccaggag gggcgctttt ggtctatact ggatcgtaat ggacggtaac
      481 gtggtattcg attccgaacg ggaccccacc ctcatgcggc gctcgagcgg ccagaaggcg
      541 ggcgtgccgc ttggcgagct catcaccccg gggccgcaga aggacaagaa gcagacggac
      601 aaggtcgtgc tcggcatcct cgggctcgta ctcatggggt cggtgtacgt ggcgatcaac
      661 acgcggccga aggagatcgt cgtgctcacc ccggccgatg cgccggtcat taacccggcg
      721 atcatcaaac cgtagtatgg cttccgctcg cggcttcacc ctgatcgagc tcctggtcgt
      781 cgtggcgatc atcgggatcc ttgcctcggt cacgctcgcg tcgctcaacg tcgcgcgcaa
      841 caaggcgcag gatgcgcgcc ggaaggccga cgtcgggcag ctcgtccgcg cgatcaacaa
      901 ctactacacc agcacgggtt cgctcccgca gggaagcggc tggtgcagct tcatatccaa
      961 caccgacagt gggcgcgacg tggcgttcag gaacgatatg gttcccgcgt acctcccgaa
     1021 ggtcccgcgc gatcccgtgc atgggggcat acccggcgat tatttctacg cgaaccagaa
     1081 cgacaaccgc ggccagttca cggtctgcgc gacgctcgcg aacgatcccg gcacgagcga
     1141 accgaacctc ttcgggtgcg ccggatgggg ctcgtacaac cattgcgtga gccaatagcg
     1201 ctcgacttct cgcgtcgctc atgatttcct agagcaggcc aatttaggca gttcggaggg
     1261 gacatgcatc catttgcgca cgagctcaca tcccgtgagc gcgccgtcat cgaatcaggt
     1321 acgacgtacc gggtcgcgac ggcatcagcg gtgctctacc acgagccaac cgatgattca
     1381 caacccatca ccaccgtgct ccacggagag catttctacg tgcagtccga gaacgaggac
     1441 ggctgggcat acggctatgc gtcggcgagc gggtggatgg ggcatcttcg gatctcggag
     1501 cttcatcgct gcgagcgcat cccgacccac cggatctgcc agctctttac gctcgtgtac
     1561 gcgcagccga cccatcgcag tcgggtcctc atgcagctcg gaatgaatgc ccgggtccac
     1621 gtgaccgatg cgacacggaa gttctaccaa gtcgcgaccg gcggatggat cccaaagacc
     1681 catctcgaga tcatcaatcg ggtctcgccg gacttcgtgg ccgaggccga gaagctcgtg
     1741 ggcatcccgt accgtccggg aggccgcgat ccgttgctcg ggctcaactg ctcaggcctc
     1801 gtgcaccacg cgctcctgac ccgcaacatc atcctctctc acggggtgcg gcacatggag
     1861 cgcgagctcg agaaggtcgc catcgatgtc tatccgcatg gtcccttcaa gcgcggcgac
     1921 ctcgtcttct gggatcgtca cgtcgggatc atggtcgacg atgcgcgcct tgtgcacgca
     1981 acgaacatcg ccatgcgcac gctcatccag cccctcgacg aggtgaaccc acggcagggt
     2041 ccgggcggca tcacgtccgt atggcggctc gccgcttaag tacgcgtaca cgaaggtccc
     2101 ggtcgctagc ttagcggccg ggcctttcgc tttccttgcc ttttacgggt ccgccccgta
     2161 tcttatgcat aacgcatctg cgggcttttt tatttagtca ttcactctat gcaacgcgat
     2221 tatcccctcg agaaagtgcg caacttcggc atcgtcgccc acgtggacgc cggcaagacg
     2281 accacgtccg agcgcgtgct cttctacacg ggctcccagc acaagatcgg ggaggtgcat
     2341 accggcgaga ccacgaccga ctggatggag caggagcgcg agcgcggtat caccatcact
     2401 tcggccgcca tcacctgctt ctggagcaag acccaggaga aggacaagaa gga
//
LOCUS       DASPKG010000019         5438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_703727, whole genome shotgun sequence.
ACCESSION   DASPKG010000019 DASPKG010000000
VERSION     DASPKG010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703727"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..912
                     /locus_tag="VEA36_00715"
     CDS             157..912
                     /locus_tag="VEA36_00715"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HYF28872.1"
                     /translation="MRQEWRKSAGRKGALMRMSLYGNPGTAAGRRLGGLRSLQSHAKL
                     NTRFKTLIPIRIPRYSVRLAELLGILMGDGHVSEYQISITTNSETDRDHARFTANLIY
                     EIFGLPARQRLRKDCKAVEILLSSKAACTFIARIGMPQGNKLKHGLVPPDWIYKSPRY
                     KHAFLRGLIDTDGTVYRDCHIINHRSYASTCIAFTSASSELRAFVKKSWLELGYHATV
                     SGRDVRLRRKADVLGYAKTIGFNNPKHSRKITV"
     gene            923..1007
                     /locus_tag="VEA36_00720"
     tRNA            923..1007
                     /locus_tag="VEA36_00720"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:957..959,aa:Ser,seq:cga)
     gene            1031..2101
                     /locus_tag="VEA36_00725"
     CDS             1031..2101
                     /locus_tag="VEA36_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28873.1"
                     /translation="MQRARFVFVLTFALTLLVQGWLWTHYPYLDSSAWASATATIRDG
                     GYPSTAQPTFGYPGTLLILPAGAIASFGVPPETANIIVMVVLLSLGAACIAACTHFLR
                     PERAWWRYVTFTLAVTPFYLISTPPTALAMLAAILVALLTLMIRERPHPGLFLALGAA
                     GALGFMSRFDTTAVVFGVALLYLLVRRERGAIMAAALAGALSILGIPMLWSGPLTYLH
                     QVLEKIGEHTQKVSASFLALDMLTEPIVFGLLGLIAGLVAYRTRVLPRDYTLWLAATT
                     VVGAGALLVSSHHPPWLFYPFLMPWMLLLPLFVLELYARSAGTLRRHAALLLAVLVLG
                     QALSLVFQVVMPVPWDAILSGP"
     gene            2098..3285
                     /locus_tag="VEA36_00730"
     CDS             2098..3285
                     /locus_tag="VEA36_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28874.1"
                     /translation="MKPAECAECGSGAVPHRLTYITILIDELTAGLIPLPKGSNPLTR
                     LVQRIERALTPHLIEFLMQAGVARRVTEPDDDTQLLALMLWNEGKKRGIEMEEFRLFN
                     LPRNIFLARLPDGRKIAFEGIPLPASELARVHWMDDKAELKKRFRALGLPVAKGGDAR
                     SVRDARRIFRSLTPPAIAKPHSGSGSRHTTLHLATEEDLVRGFRIAQEVSPRVVIEEE
                     LVGPVYRATVVDGTFAAALRRDQPYVTGDGKRSVRELVEEANTHPARSGPYFHNIQLD
                     EAAAEELAWQGLATDSVPEAGRRVTLHQKVNWSVGGTTADVTDEVHPYNIALFEEVAR
                     VLKAPIVGIDFIIEDIARPWKEQERCGILECNSMPFFDNHHLPFEGKPRNVAALIWQM
                     NGV"
     gene            3415..3540
                     /locus_tag="VEA36_00735"
     CDS             3415..3540
                     /locus_tag="VEA36_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28875.1"
                     /translation="MDQDGATDNGDQVAEEVTVDQPAEEGMGADAAPEAGEPAAE"
     gene            complement(3591..4517)
                     /locus_tag="VEA36_00740"
     CDS             complement(3591..4517)
                     /locus_tag="VEA36_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYF28876.1"
                     /translation="MHDLIIIGGGPAGVAAGVYAARKRLKSVLVTEEFGGQSTVSEGI
                     ENWIGTVRIAGPELAHSLKAHLEAYKGDVVEVVEGDRVEKLEKAADGFSVSTRDGKSF
                     AGRAVLIASGAGRKKLAIPGADTFENKGVTYCASCDGPLFSGQDVVVVGGGNAGFETA
                     AQLLAYCKSVTLIHRRDSFKADAITVEKVLAHPNMTALTNTEPVEVKGDKFVSGLVVK
                     NIETGETRELAITGVFAEIGVIPNTDYARGVVDLDTVGRVVTNPRNQTTSVEGIWAAG
                     DATDELYHQNNIAAGDAVKALEDIYQWTMRNR"
     gene            complement(4569..>5438)
                     /locus_tag="VEA36_00745"
     CDS             complement(4569..>5438)
                     /locus_tag="VEA36_00745"
                     /inference="COORDINATES: protein motif:HMM:NF023746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HYF28877.1"
                     /translation="VGVVTRPFAFEGLQRKEIAEHGLSDLKQEVDAFIVIPNDKLLSI
                     VEAQTTAKNAFALADEILRQAVEGVSDIITTPGEINTDFNDIKAIMEGAGPALMGIGI
                     ADGDRRAQDAAAQAVNSPLLDVSITGAKGVLFVVAGSDDLGILEVQEAARVVSDSVDK
                     NAKVIFGIMRDEKLKKGQIRIIVIATGFPEGDGDSQQGGIERSLFAMPAEEQQETRGK
                     VFADLIRRPAPVIIEETVMVAEEEPIREERHEPVVTAQPHRREEVRPLTPPAEEEEEA
                     WGAIPSFLRRHKK"
BASE COUNT          950 a         1751 c         1639 g         1098 t
ORIGIN      
        1 tcgaaccccg atttacgccg tgaaagggcg ctgtcctagc cgttagacga tggggccttc
       61 tttgtgaagg cggcacgcca tagatgctac tactttttta acactgcaac aatgcatatt
      121 tgtcagaact gttaatatat aggtactcga aataatatgc ggcaggaatg gcgtaaatct
      181 gcaggaagga agggtgccct catgagaatg tcgctctacg gtaatcccgg tactgcggcg
      241 ggaaggaggt tgggcggact gcgctctcta cagagccatg caaagctcaa cacacgcttt
      301 aagacgctca tacccatacg gatcccgcgt tattcggtgc gtcttgcaga attgctaggg
      361 attcttatgg gtgatgggca tgtgagtgag tatcagatat ccattacgac taattcagaa
      421 actgatcgtg accacgcgcg atttactgca aatctcattt atgagatttt cgggttacct
      481 gcacgtcagc gattgcgcaa ggattgcaaa gcggtagaaa tcctcctttc ctctaaagca
      541 gcctgcacct tcattgctcg catcggcatg ccgcaaggta ataagctgaa gcatggtctt
      601 gttccacctg attggattta taaatcgcca cggtataaac acgcattcct gcgggggctt
      661 atcgataccg atgggactgt ctatcgcgac tgccatatca tcaatcacag gagttacgcc
      721 tccacatgca tcgccttcac aagtgcatcc agtgagttgc gagccttcgt aaagaaatca
      781 tggctggagc tcggatacca cgcgaccgtt tctggccgag acgtacggct gcgtaggaag
      841 gcagatgtgc tcggctacgc caagacgata ggcttcaata accctaaaca ttctcgtaag
      901 attacggtat agtgcctccc acggaggggt cgcatagtgg tctagtgcag tggtttcgaa
      961 aaccaccata ccgcaaggta tcgggagttc gaatctcccc ccctccgcac acgctatact
     1021 gaaccgcacc atgcagcgtg cgcgcttcgt cttcgtcctc accttcgccc tgacgctgct
     1081 cgtgcagggc tggctctgga cgcactaccc ctacctcgat agctccgcat gggctagcgc
     1141 caccgcgacc atccgcgacg gcggctatcc atcgaccgca cagccaacct tcgggtatcc
     1201 cggaacgctc ctcatcctcc cagcgggagc gatcgcatcc ttcggtgtcc ctcccgagac
     1261 cgcgaacatc atcgtgatgg tggtgctgct ctcgcttggt gccgcctgca tcgcggcctg
     1321 cacccatttc cttcgcccgg agcgtgcctg gtggcgctac gtcacgttca cgcttgccgt
     1381 gacgcccttc tacctgatca gcaccccgcc gaccgcgctt gcgatgcttg ccgcgatcct
     1441 cgtcgcgctc ctgaccctca tgatccggga gcgcccccat cccgggctct tcctcgcgct
     1501 tggcgctgcg ggcgcgctcg ggttcatgag ccgtttcgat accacggccg tcgtcttcgg
     1561 ggttgcgctc ttgtatctcc tcgtccggcg ggagcgggga gcgattatgg cagcggcgct
     1621 cgcgggcgcg cttagcattc tcggcatccc gatgctctgg tccggaccgc tgacgtactt
     1681 gcaccaggta ctggagaaga tcggcgagca cacgcagaag gtgtccgcct ccttccttgc
     1741 gctcgatatg ctcaccgagc ccatcgtctt cggtctgctc gggctcatcg cggggctcgt
     1801 ggcgtaccgc acacgcgtgc tgccgaggga ctataccctc tggctcgccg ccacgacggt
     1861 cgtcggcgcc ggcgcgctcc tcgtctcgag ccatcatccg ccctggctct tctatccgtt
     1921 cctcatgcct tggatgcttc tcctcccgct cttcgtgctc gagctctacg cgcggtccgc
     1981 aggaacgctg cgccgccatg ccgcgctcct gctcgcggtc ctcgtcctcg gccaggccct
     2041 ctcgctcgtc ttccaggtgg tgatgcccgt gccctgggac gctatactgt ccgggccatg
     2101 aagcccgccg aatgcgccga gtgcggatcc ggagccgtcc cgcatcgcct cacgtacatc
     2161 accatcctca tagacgagct caccgcaggg ctcatcccgc tcccgaaggg ttcaaacccc
     2221 ctcacccgcc tcgtccagcg catcgagcgc gccctcaccc cgcacctcat cgaattcctc
     2281 atgcaggcgg gggtcgcgcg gcgcgtcacg gagcccgacg acgataccca gctgctcgcc
     2341 ctcatgcttt ggaacgaagg gaagaagcgg ggcatcgaga tggaggagtt ccggctcttc
     2401 aacctgccgc gcaatatctt cctcgcccgg ctcccggacg ggaggaagat cgccttcgag
     2461 ggcatccccc tgcctgcctc cgagctcgcc cgcgtgcatt ggatggacga taaggcggag
     2521 ctgaagaagc gttttcgcgc gctcgggctc ccggtggcca agggcggcga tgcgcgctcc
     2581 gtacgggatg ccaggcgcat cttccgttcc ctcacgccgc ccgcgatcgc caagccccat
     2641 tcgggatcgg gctcgcggca caccacgctc catcttgcga ccgaggagga tctggtccgg
     2701 ggcttccgta tcgcgcagga agtctcgccg cgcgtcgtca tcgaggaaga gctcgtgggg
     2761 cccgtctacc gtgctacggt tgtggacggc acgttcgcgg cagcgctccg gcgcgatcaa
     2821 ccctacgtca ccggagacgg gaagcgcagc gtgcgggagc ttgtcgagga ggcgaacacg
     2881 catccggccc gcagcggccc gtatttccat aacatccagc tcgatgaggc agcagccgag
     2941 gagctcgcct ggcaggggct tgcgacggac tcagtccctg aagccggaag aagggtaacg
     3001 ctccaccaga aggtgaactg gtcggtcggc ggcacgaccg cggacgtcac cgacgaggtg
     3061 catccgtaca acatcgcgct cttcgaggag gtggcgcgtg tcctgaaggc gcccatcgtc
     3121 ggcatcgact tcatcatcga ggacatcgcg cggccctgga aggagcagga gcgctgcggc
     3181 atccttgagt gcaactccat gcccttcttc gataaccacc acctcccgtt cgaaggcaag
     3241 ccccggaacg tcgctgccct gatatggcag atgaatgggg tctagcgttc attgcttttt
     3301 caaggtcatc cttggtatag tcgggggaca ggtcgaattg ccagccaact aacgattgga
     3361 tggcttctgc ttttttgcgg gagccgtcct ccaacaccta tgatgaacga accgatggat
     3421 caggatggtg ctaccgataa cggcgatcag gtcgcagaag aagtgaccgt ggatcagccg
     3481 gcggaagagg gcatgggcgc cgatgcggcc ccggaggctg gcgagccggc agctgagtaa
     3541 gcaaccgact cccacaaaag aagaaccgct cctccggagc ggcttttctt ttatcggtta
     3601 cgcatcgtcc actggtagat gtcctcgagc gccttcacgg cgtcgcctgc cgcgatgttg
     3661 ttctggtggt agagctcatc ggtggcgtcg cccgcggccc agatgccctc gaccgaggtc
     3721 gtctggttcc tcgggttcgt aacgacgcgc cccaccgtat cgaggtctac gacgccgcgt
     3781 gcgtagtcgg tgttcgggat cacgccgatc tcggcgaaga ccccagtgat ggcgagttcg
     3841 cgcgtctccc cggtctcgat attcttcacg acgagtcccg agacgaactt atcgcccttc
     3901 acctccaccg gttcggtgtt cgtgagcgcg gtcatattcg gatgcgcgag caccttctcg
     3961 accgtgatcg cgtccgcctt gaacgaatcg cggcggtgga tcagggtaac ggacttgcag
     4021 taggcaagga gctgcgcggc cgtctcgaag cccgcgttgc cgccgccgac caccaccacg
     4081 tcctgtcccg agaagagggg gccatcgcag gaggcgcagt acgtcacgcc cttgttctcg
     4141 aaagtatccg ctccgggaat ggcgagcttc ttgcggccgg cgcccgaggc gatcaggacc
     4201 gcgcggcccg caaatgactt gccgtcgcgg gtggagacgg agaagccgtc cgctgccttc
     4261 tcgagcttct cgacccggtc tccttcgacc acctccacca cgtctccttt atatgcttcc
     4321 agatgcgcct tgaggctgtg cgcgagctcg ggacctgcaa tgcgcaccgt gccgatccag
     4381 ttctcgatcc cttcggagac ggtagactgc ccgccgaatt cctcggtgac aaggacgctc
     4441 ttcaagcgtt tgcgggcggc gtatacgcct gcggcgacgc ctgcgggccc gccgccgatg
     4501 atgatgagat cgtgcatgtg caggcagtat agcaaaatcc ccgagagggt cccggggatt
     4561 cgcttcggct atttcttatg ccttcgcagg aaagagggga tcgcgcccca tgcctcctcc
     4621 tcttcctcgg caggtggagt cagtggccgt acttcctcgc ggcgatgcgg ctgcgcggtc
     4681 acgacgggtt catggcgctc ctcacggatc ggctcctcct cggcgaccat gacggtctcc
     4741 tcgatgatga ccggcgcggg gcggcgaatg aggtccgcga agaccttgcc gcgcgtctcc
     4801 tgctgctctt cggcgggcat cgcgaagagc gatcgctcga tgccgccctg ctggctgtcg
     4861 ccatcgccct cggggaagcc ggtcgcgatg acgatgatcc ggatctggcc cttcttgagc
     4921 ttctcgtccc gcatgatacc gaagatgacc ttcgcgttct tgtcgacgga atccgagacc
     4981 acgcgtgcgg cctcctggac ctcgaggatg ccgaggtcgt ctgagccggc gacaacgaag
     5041 agcacgccct tggcgcccgt gatggagacg tcgagaagcg gggaattgac ggcctgcgcg
     5101 gcggcgtcct gggcgcgacg gtcgccgtcg gcgatgccga tgcccatgag ggcggggccg
     5161 gcgccttcca tgatggcctt gatgtcattg aagtccgtat tgatctcgcc cggggtggtg
     5221 atgatgtcgg agacgccctc caccgcctgg cgaaggatct catcggcgag cgcaaaggcg
     5281 ttcttcgcgg tcgtctgcgc ctcgacgatg gagaggagct tgtcgttcgg gatcacgatg
     5341 aaggcgtcca cttcctgctt gaggtcggac aggccgtgct cggcgatctc cttgcgctgg
     5401 agcccctcga aggcgaaggg gcgggtgacg acgccgac
//
LOCUS       DASPKG010000020         4322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1656330, whole genome shotgun sequence.
ACCESSION   DASPKG010000020 DASPKG010000000
VERSION     DASPKG010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656330"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VEA36_00750"
     CDS             complement(<1..253)
                     /locus_tag="VEA36_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28878.1"
                     /translation="MSWLKNLLNPPSKPTTSAAPVFFTNTATGKKEIFSPLRAGQVLM
                     YSCGPTVYSKQHIGNLKAPLFSDLVARVLTQAGYRTKRVM"
     gene            complement(351..971)
                     /locus_tag="VEA36_00755"
     CDS             complement(351..971)
                     /locus_tag="VEA36_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28879.1"
                     /translation="MRPSSSAATAASIPVLTSIVERIAAFTADQFIPPERPAPETPAQ
                     IVGQLNEHQRRLWTLQIQVLEEHNAKRREVNGLRKQVSDATKQGRNALQILKNLLGGG
                     LTKDVVERLEKAEYELTLLDLTHQVLKGLFWLEVQTQYGELADKPSIALYNDWTVGWQ
                     EVEELDLFGLGGVEFDIVGEMPAGRLAEILRGSRRHTRQRYPAGVH"
     gene            complement(1053..1967)
                     /locus_tag="VEA36_00760"
     CDS             complement(1053..1967)
                     /locus_tag="VEA36_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HYF28880.1"
                     /translation="MIMRHARGAVTWVDLEAPTPEELSAVMAEFDIDPRIEEEIARPT
                     PYPMTLSMPEYEYLILHFPAVEEQEGTKNQEVDFIVGKEFLITARYEVIESIHALHKV
                     FETEELAGEVREAHADALLERVMIRLYAAIHDEIEREGRLLEHIEHDIFSGKERQTVR
                     TISHAGRALLRFETALNRHEEPLDEFLAALAVPEFFGKRFAARAARIRAAHDHAAALV
                     GSYRAVLRELRITNDSLLSTSQNEVMKTLSLMAIVALPMTLVASVFGMNATYMPFVHE
                     PFGFWTILGLMVIASLLVFLFFKVKRWF"
     gene            2041..3285
                     /locus_tag="VEA36_00765"
     CDS             2041..3285
                     /locus_tag="VEA36_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYF28881.1"
                     /translation="MEGDRSPFKRMTFQAVALAIALAVGFGGGVLAAGADVDRAFSQI
                     PLIGDGLDATPDESVDLADFWKAWNALDARFVETSASSTAPSAKEKLWGAIQGLAQSY
                     GDPYTVFMPPEEAKVFQDDISGNFEGVGMEIGIKDDLLTVIAPLKGTPAERAGIRAGD
                     GILSINETPTDGLSTDEAVKLIRGPKGSEVRFKLYRDGNVVEVTVIRDRIEVPTIDHG
                     LDQATGVYTISFYSFTANSDDLFARAVAGFRASDSDKLLIDLRGNPGGYLEAAVRISG
                     HFLPEGATVVTEDYKGKRENIVHRSRGQGGVPSNTRVVILINQGSASASEILAGALQD
                     EKRAVLIGERSFGKGSVQELVKVGGGALKVTVARWLTPAGRSISDGGLMPDIEVKRTP
                     EDVTAGKDPQKDRAVEFLTTGE"
     gene            complement(3343..3930)
                     /locus_tag="VEA36_00770"
     CDS             complement(3343..3930)
                     /locus_tag="VEA36_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28882.1"
                     /translation="MTTPIDPRVRTILENQAYALDIYDVRSPSAMFNAAAALNRKRRP
                     QGFRYVDYKGRTAYNFLIVAGKDGAEDEFLGGWISQTDKYGLDTEAAVSEVTDCELPS
                     LVHRFTRAVERSYRSRRSSSYQSGYLLGILPVVLIGGVVAGVYGVSLDKSSAWALIPI
                     LGLLYGLTWVASHVHLHFAWKKDETAGALRALSAA"
     gene            4070..>4322
                     /gene="rplI"
                     /locus_tag="VEA36_00775"
     CDS             4070..>4322
                     /gene="rplI"
                     /locus_tag="VEA36_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HYF28883.1"
                     /translation="MKVVLLKDVKNMGRAHTAIDVSDGHALNFLIPRKLAVPATPSSL
                     KQAELRVKQVKDRKELDVKLVEGRLAQLADEKVTITKKAN"
BASE COUNT          816 a         1358 c         1408 g          740 t
ORIGIN      
        1 tcatcacgcg cttggtgcgg tatccggcct gcgtaagcac gcgcgcgacg aggtccgaga
       61 acagcggcgc cttcaggttc ccgatatgct gcttgctgta tacggtcggg ccgcaggaat
      121 acatgaggac ctgcccggcc cgcagcggcg agaagatctc cttcttgccg gtcgcggtat
      181 tggtgaagaa gaccggagcg gcggatgtgg tgggctttga gggcgggttg aggagattct
      241 tcagccagga cattacggga ccattgtacc gcagtgcaga gtataaaaaa cacgcggacc
      301 cggaggcccg cgtgcgaacc acttgcggtt cgaaaaagtc gaacaggaac tcagtggact
      361 cctgccgggt accgctggcg cgtgtggcgc cgggagccgc ggaggatctc ggcgagccgc
      421 ccggccggca tctcgccgac gatatcgaac tcgacgcctc ccaggccgaa cagatcgagc
      481 tcctcgacct cctgccagcc gacggtccag tcgttgtaga gggcgatcga gggcttatcg
      541 gccagttcgc cgtactgcgt ctggacctcg agccagaaca ggcccttcag gacctggtgc
      601 gtcaggtcga gcagggtcag ctcgtactcg gccttctcga ggcgctcgac cacgtccttg
      661 gtcaggccgc cgccgaggag gttcttcagg atctgcaggg cattgcggcc ctgcttggtc
      721 gcgtcggaga cctgcttgcg caggccattg acctcacggc gcttcgcgtt gtgctcctcc
      781 agcacctgga tctgaagggt ccagaggcgc cgctggtgct cgttcagctg gccgacgatc
      841 tgcgcgggcg tctccggcgc cggcctttcg ggcgggatga actggtccgc ggtgaacgcg
      901 gcaatccgct cgacgatcga cgtcaggacg ggaatcgaag ccgcggtcgc ggcggaactg
      961 ctggggcgca tcgaaggctc cttcgtgatg ctgtctcggg cccaatatga cccgaaaaat
     1021 ctataacaca ctatctaaat atacgcaaat ccctagaacc accgcttcac cttgaagaag
     1081 aggaagacga gcaacgaggc gatcaccatg agccccagga tcgtccagaa tccgaatggc
     1141 tcgtgcacaa agggcatata cgttgcattc atgccgaaga cggaggcaac gagcgtcatc
     1201 gggagcgcga cgatggccat gagggagagc gtcttcatta cctcgttctg cgaggtggaa
     1261 aggagggaat cgttcgtgat gcgcagctcg cgcaggaccg cgcggtagct gcctacgagc
     1321 gcggccgcat ggtcatgcgc ggcccggata cgcgcggccc gcgcggcgaa gcgcttcccg
     1381 aagaactccg gcaccgcaag cgcggcgagg aactcatcga gcggttcctc atggcggttg
     1441 agcgcggtct cgaagcgcag caatgcgcgc ccggcatgcg agatggtacg gaccgtctgc
     1501 cgctcctttc cggagaaaat atcgtgctcg atgtgctcga ggaggcgtcc ctcccgctcg
     1561 atctcgtcgt ggatggcggc atagagccgg atcatgacgc gctccaagag ggcatcggca
     1621 tgggcctcgc ggacctcgcc ggcgagctcc tcggtctcga acaccttatg gagcgcatgg
     1681 atggactcga taacctcgta gcgggcagtg atgaggaact ccttgcccac gatgaagtcc
     1741 acctcctggt tcttcgtgcc ctcctgctcc tcgacggcag ggaagtggag gatgaggtat
     1801 tcgtactccg gcatcgacag cgtcatgggg tagggggtcg gccgcgcgat ctcctcctcg
     1861 atccgggggt cgatatcgaa ctccgccatc acggcggaga gttcctcggg ggtcggcgcc
     1921 tcaaggtcca cccaggtcac cgcgccgcgg gcatgtcgca tgatcatgag ctcaagtata
     1981 ccctccaggg cgcgcgcatg agcccggaat ccccggcccg catgatagaa tgcagcgaac
     2041 atggagggag accgttcacc attcaagcgc atgacgttcc aggcggtcgc gcttgcgatc
     2101 gcgctcgccg tcggattcgg cggcggcgtg cttgccgcgg gcgccgacgt cgacagggcc
     2161 ttttcgcaga tcccgctcat cggcgacggg ctcgacgcca cccccgacga gtcggtggac
     2221 ctcgcggact tctggaaggc ctggaacgcg ctcgacgccc gcttcgtcga aacgagtgct
     2281 tcctcgaccg cgccctcggc caaggagaag ctctggggcg ccatccaggg ccttgcgcag
     2341 tcctatgggg acccgtatac cgtcttcatg ccgcccgagg aagcgaaggt cttccaggac
     2401 gacatctccg ggaacttcga gggcgtcggg atggagatcg gtatcaagga cgacctcctg
     2461 accgtcatcg cgcccctgaa gggcacgccc gccgagcgcg cgggcattag ggccggcgac
     2521 ggcatcctct cgatcaacga gacgccgacc gacgggctct cgaccgacga ggccgtaaaa
     2581 ctcatccggg gccccaaggg cagcgaggtg cgcttcaagc tctaccgcga cgggaacgtc
     2641 gtcgaggtga ccgtcatccg cgaccgcatc gaggtgccga ccatcgatca cggcctcgac
     2701 caggcgaccg gcgtctacac catctccttc tacagcttca cggctaattc cgacgacctc
     2761 ttcgcgcgcg ccgtcgcggg cttccgggcg tccgactccg acaagctcct catcgacctc
     2821 cgggggaacc cgggcgggta tctcgaggct gcggtgcgca tatccgggca cttcctcccc
     2881 gagggcgcga ccgtcgtgac cgaggactac aaggggaagc gcgagaacat cgtgcaccgg
     2941 agccgcgggc agggaggggt cccgagcaat acgagagtcg tcattttgat aaaccaggga
     3001 tcggcgtccg cctccgagat ccttgctggc gcgctccagg acgagaagcg ggcagtgctc
     3061 atcggcgagc gatccttcgg gaagggctcg gtgcaggagc tcgtgaaggt gggcggggga
     3121 gcgctcaagg tgacggtggc gcgctggctc acgcccgcag gccgctcgat ctccgatggc
     3181 ggcctcatgc cggatatcga ggtgaagcgc actcccgaag acgtgaccgc cgggaaggac
     3241 ccgcagaaag atcgcgcggt agagttcctg acgaccgggg aataggaaaa ggacgcgtcc
     3301 atcgctggta cgcgccctca ttcgttacgc ctcgtggcgg acttacgccg cggacaacgc
     3361 cctgagggcg ccggccgtct cgtccttctt ccacgcgaag tggagatgga cgtggctggc
     3421 cacccaggtg aggccgtaca gcagaccgag gatcgggatc agggcccacg cgctgctctt
     3481 atcgagcgac acgccataga cccccgcgac gacaccgccg atgagcacga cgggtaagat
     3541 cccgagcagg tagccgctct ggtagctcga cgaccgcctc gaccggtagc tccgctccac
     3601 cgcgcgagtg aagcggtgga cgagcgaggg aagctcacag tcggtcacct cgctcacggc
     3661 agcctcggtg tctaagccgt acttgtcggt ctgcgagatc cagccgccga ggaactcgtc
     3721 ctcggcgccg tccttgccgg ccacgatcag gaagttgtaa gccgtgcgcc ccttgtagtc
     3781 gacgtaccgg aagccctgcg gcctccgttt ccggttgagc gctgcggcgg cgttgaacat
     3841 cgccgagggc gaccggacgt cgtagatgtc gagcgcgtag gcctggttct ccaggatcgt
     3901 gcgaacccgc gggtcgatgg gcgtggtcat gcccgttctc cttgttgcta ttgagtcaaa
     3961 gatacttacg cacgtcattg cacttcgact caatataagc acactcttta tatattgtca
     4021 actatgcgga tttgcgtaat acccgcctaa acgctacgat gtctccacta tgaaagtcgt
     4081 actactcaaa gacgtgaaga acatgggccg ggcgcatacc gcgatcgacg tgtcggacgg
     4141 gcatgctctc aatttcctca tcccccgcaa gctcgcggtc ccagcgactc cgtcgagcct
     4201 gaagcaggcg gaactgcgcg tgaagcaggt caaggatcgc aaggagctcg atgtgaagct
     4261 cgtcgaaggg cgtctggcgc agctcgcgga cgagaaggta accatcacca agaaagcgaa
     4321 cg
//
LOCUS       DASPKG010000021         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_305798, whole genome shotgun sequence.
ACCESSION   DASPKG010000021 DASPKG010000000
VERSION     DASPKG010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305798"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VEA36_00780"
     CDS             complement(<1..663)
                     /locus_tag="VEA36_00780"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HYF28884.1"
                     /translation="MRPEIIKADGTIEPFDGQKLETSLRKAGASEKTAERIRHTIESS
                     IAPAAESTEVYRRAFQMLRREARPAAARYSLRRALFEFGPSGHPFEDFVAEIFKKEGW
                     AVEGRRVIPGKCVPHEVDVFATRGQERIAAELKYHNDPGYKTDVKVALYVKARFDDIW
                     QCDPKSQSCPIDRGFLITNTKFTSHAVQYAKCSGLGLLGWSYPSQGNLYDRIVASGVY
                     PIT"
     gene            complement(664..1698)
                     /gene="mnmA"
                     /locus_tag="VEA36_00785"
     CDS             complement(664..1698)
                     /gene="mnmA"
                     /locus_tag="VEA36_00785"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HYF28885.1"
                     /translation="MNLSGTRVFVGLSGGVDSAVTAALLKDAEAEVVGVFIKGWYPPG
                     MPCTWAADRRDAMRVAAALNIPFHTLDASAEYRRSVIEYLLAEYRAGRTPNPDIMCNR
                     EVKFGAFYRFARERGADLIATGHYAQVVEGKLFRGSDEAKDQSYFLWAVPSETLMHTV
                     FPLGAQTKGDTRSLARRHRLPVATKKDSQGICFLGSISVEDFLRSEFGEEPGEARDAK
                     GRVIGRHNGALLHTIGERLSLTGAVPGPWYVVGKDIETNVLIVSRTSMVVRSDPRLRL
                     TETNWLAEPGASETLTAQYRYHGPEVRGTLAADRTYFTPADPLPEAIAPGQSLVVYRG
                     NQCIGGGIIG"
     gene            1806..2066
                     /locus_tag="VEA36_00790"
     CDS             1806..2066
                     /locus_tag="VEA36_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28886.1"
                     /translation="MRLRVLLGQRLHAGDPHDTNRRCMFPSGTILDGGVERIGRRKWL
                     MFGSPSGFGLPLRKQPSERHIISLGEAIRREVVSAEPLEYGA"
     gene            complement(2137..>2429)
                     /locus_tag="VEA36_00795"
     CDS             complement(2137..>2429)
                     /locus_tag="VEA36_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28887.1"
                     /translation="NAENTNALGITRWDAIFNDYPWEDRSGKHKEHLEHQLVDAYRRR
                     SYFTEPYRLPWVVMSSEELATIFHIPSASVTAPSLPRIQSSTSEAPSNLPTG"
BASE COUNT          420 a          794 c          774 g          441 t
ORIGIN      
        1 cgtgatggga tacaccccag acgccacgat gcggtcgtag aggttcccct gcgagggata
       61 gctccagccg agcaggccca gccccgagca cttcgcgtac tgcaccgcat ggctcgtgaa
      121 cttggtgttg gtgatcaaaa atccccgatc gatcgggcag gactggctct tcggatcgca
      181 ctgccatata tcgtcgaatc gcgccttcac gtagagcgcg accttcacgt ccgtcttgta
      241 gcccggatcg ttgtggtact tcaattccgc cgcgatgcgc tcctgcccgc gcgtcgcgaa
      301 cacgtccacc tcatgcggca cgcacttgcc ggggatgacc ctcctcccct ccaccgccca
      361 tccctccttc ttgaagatct cggcgacgaa gtcctcgaag ggatgccccg agggcccgaa
      421 ttcgaagaga gcgcggcgaa ggctgtagcg cgcggccgca gggcgcgcct cgcgccgcag
      481 catctggaac gcgcgccggt acacctcagt ggactcggcc gccggcgcga tcgaggactc
      541 gatggtgtgc cgaatgcgtt cggccgtttt ctcggatgca ccagccttcc ggagcgatgt
      601 ctcgagcttc tggccatcga agggttcgat ggtgccgtcc gccttgatga tctcaggcct
      661 cattcacccg atgataccac caccgatgca ctggttgccg cggtatacga cgagcgattg
      721 gccaggtgct atcgcttcgg ggagcggatc cgcgggagtg aagtacgtgc gatcggccgc
      781 aagcgtcccg cgcacctccg gaccgtggta ccgatactgc gcggtgagcg tctcggaagc
      841 cccgggctca gcgagccaat tcgtttcggt gaggcgcaag cgcggatcgc tgcgtaccac
      901 catcgatgtc ctcgagacga tgagtacgtt cgtctcgata tccttgccga cgacgtacca
      961 ggggccgggc accgctcccg taagcgagag gcgctcccct atggtatgca ggagcgcgcc
     1021 attgtgccgg ccgatcaccc gtcccttcgc atcccgcgcc tccccgggct cctccccgaa
     1081 ctcgctacgc aggaaatcct cgacggatat tgaaccgagg aagcagatcc cttggctatc
     1141 cttcttggta gcgacgggca gccggtgcct gcgcgccagc gaccgcgtgt ctcccttcgt
     1201 ctgcgccccg agcgggaaca ccgtatgcat aagcgtctcg ctcggaaccg cccagaggaa
     1261 gtagctctgg tccttcgcct cgtcgctacc ccggaatagc ttgccctcca cgacttgggc
     1321 atagtgccca gtcgcaatga ggtccgcgcc ccgctcccgc gcgaatcggt agaaggcgcc
     1381 gaacttcacc tcccggttgc acataatgtc cgggttcggc gtgcggcccg ccctatattc
     1441 ggcaaggagg tattcgatga cgctgcgccg gtactccgcg ctcgcatcga gcgtgtggaa
     1501 ggggatgttg agcgcagccg cgacccgcat cgcatcgcgg cgatcggctg cccacgtgca
     1561 gggcatgccg ggtgggtacc agcccttgat gaataccccg accacctctg cctccgcgtc
     1621 cttcaggagc gcggccgtca ccgcagagtc caccccgcct gaaagcccga cgaataccct
     1681 tgttccagaa agattcatga cccagtcata gcatcgttga cagaatcacg aaagttgcga
     1741 tgctacgggt aaccacggaa aggaggtgat cccacgtggg ttctttagga aggaatgtct
     1801 cgcgcgtgcg cctcagggtg cttctcggcc agcgactgca cgccggtgat ccgcacgaca
     1861 ccaataggcg ctgcatgttc ccgagcggca ccatcctcga cggcggggtc gagcggatcg
     1921 ggcggcgaaa atggctgatg ttcgggtcgc cgagcggctt cgggcttccg ctcaggaagc
     1981 agccgagcga gcggcacatc atcagcctcg gggaggcgat acgccgtgag gtcgtgagcg
     2041 ctgaaccgct cgagtacggg gcgtaacggg aggagcctga tgcgcaagca aggcgggggc
     2101 cgcggaatgc agatacctgc gccgcggcct ttcctattat cccgtcggca ggttggacgg
     2161 cgcctcggac gtcgaggact ggatgcgcgg cagggacggc gcggtcacgg aggcgctcgg
     2221 gatatggaag atggtcgcga gctcctccga gctcatcacc acccagggta ggcggtacgg
     2281 ctcggtgaag tacgagcgcc ggcggtacgc gtccacgagc tggtgctcga ggtgctcctt
     2341 gtgcttcccg ctgcggtcct cccagggata gtcgttgaag atggcgtccc agcgcgtgat
     2401 gccgagcgcg ttcgtgttct cggcgttca
//
LOCUS       DASPKG010000022         9044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1523898, whole genome shotgun sequence.
ACCESSION   DASPKG010000022 DASPKG010000000
VERSION     DASPKG010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9044)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9044
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523898"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VEA36_00800"
     CDS             <1..251
                     /locus_tag="VEA36_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYF28888.1"
                     /translation="MTLGLLVAGLYVVINQLEAHVIHPLVVNKVVGIPPLLVILALIV
                     GGTLAGFLGVLLSVPLAAAAREFLADVERGKKAKATVS"
     gene            253..1695
                     /gene="metG"
                     /locus_tag="VEA36_00805"
     CDS             253..1695
                     /gene="metG"
                     /locus_tag="VEA36_00805"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HYF28889.1"
                     /translation="MARYITTTLPYVNADPHIGFAVELVQADSLVRLWRAGGEAVFFN
                     TGTDEHGQKIYEAAQKAGRDVQEYVDHYAAEFRKLKDVLSLDNPTFIRTTDAHHKQAA
                     AEMWRRCEAAGDIYKKSYEGLYCVGCELYKNEKDLTGDGHCLVHPHLKPELVSEENYF
                     FRFSKYQDALIEYLSRADVVLPDWRREEALAFAKGGLEDFSISREKARLSWGIPVPGD
                     ESQVMYVWFDALTDYISTLGWPEDADGFFEKFWQNGRTLQMAGKDQVRFQSLMWQAML
                     MSAGVKSTDQVFYHGFINSGGQKMSKSLGNVISPYELVEKYGTDATRYLLLRHVHPTE
                     DSDVTWERLDEWYTANLVNGLGNLVARVMKLAEEHLPGPVILTELDTSTEFAFTEKLE
                     AFKFNEAMDLIWEHIAKGDEYMTTEEPYKKVKEEATKVEAIAVIEKLVRHLAKLAAHI
                     EVVMPSTAEVIRAAVRENRKPENLFPRLDK"
     gene            1865..2641
                     /locus_tag="VEA36_00810"
     CDS             1865..2641
                     /locus_tag="VEA36_00810"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HYF28890.1"
                     /translation="MKYFDAHCHIQFEKYDGDRTALIEQLVQEGVGGLVVGTDLASSR
                     AAIELVEDLDGFWATVGLHPNHVNDETFDDGIYRGLAMHPKVVAIGECGLDNYRPGDV
                     EAAKPLQRDLFRRQVELSLEADKPLMIHGRPAKGGMDAYEDLLAVLAEYPGARGNIHF
                     FVGTPAIARRFIDLGFTLSFTAVITFARDYDDVIRSIPLDSILSETDSPYVAPAARRG
                     QRNDPRSAKDVVAALAQIRGEDEEVVRAAVLRNARRVFSL"
     gene            2680..3183
                     /locus_tag="VEA36_00815"
     CDS             2680..3183
                     /locus_tag="VEA36_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HYF28891.1"
                     /translation="MNKDIEHGTDGTELELGEGYNESRIYELGFHIDPELPQEEVKKV
                     YQGIRETAEKAGSLVAEGEPQRIPLAYTISRMEPSGRRDFNSAYFAWVAYEADGQGHD
                     EMLRLAKEHPALVRYLDVKTDKEAAKHAAEMHEIYAKLGEKEEGQTSEEEVSDVELEA
                     ALKDAEI"
     gene            3200..3658
                     /gene="ssb"
                     /locus_tag="VEA36_00820"
     CDS             3200..3658
                     /gene="ssb"
                     /locus_tag="VEA36_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HYF28892.1"
                     /translation="MYLNKVLLYGNLTRDPELKALPSGQQVASFGIATNRTFTNKEGQ
                     KQEQTEFHNIVAFGRTAEVMGQYLKKGRPVFIEGRLQTRSWESEGKKNYRTEIVVDTF
                     QFGPQAGGAGGDRSAYAAGGAAAGAVAAPEAAASAIEYPEEEINPEDIPF"
     gene            3672..3878
                     /gene="rpsR"
                     /locus_tag="VEA36_00825"
     CDS             3672..3878
                     /gene="rpsR"
                     /locus_tag="VEA36_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HYF28893.1"
                     /translation="MAYQAEREEIENKKFVDYKDVAYLRQFTNPHAKILGRKRTGIPA
                     EKQRQISLAIKRARYMALMPYLAA"
     gene            4013..4507
                     /locus_tag="VEA36_00830"
     CDS             4013..4507
                     /locus_tag="VEA36_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28894.1"
                     /translation="MRLKSSHAWLLALAAVIPILSVQYLRVQSAENHLNAAQWAARCV
                     KAPRWAVEELATKFPHHGEAAVQVAGFAGSGRKGIKVSVNEAGTPDCQTDYAPDYGQV
                     IFAADRQPMAAYLTKPGSNGIVHMFAIMELAPDPELSGKRIFVIFTGHFGPPGTKPQR
                     KIQT"
     gene            complement(4574..5149)
                     /locus_tag="VEA36_00835"
     CDS             complement(4574..5149)
                     /locus_tag="VEA36_00835"
                     /inference="COORDINATES: protein motif:HMM:NF019814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28895.1"
                     /translation="MAILSYNEILPKSVIVYNSEPYEVLSSHIFRMQQRKPVNQTKLR
                     HLVSGKVLEISFHQSETVPEADISKMQATYLYTNRGESWFTEEGNPKNRFAFPEDAVA
                     DKVMWLKQNSPVEVLLYNEKPVSVKIPIKMDLKVKEAPPAVRGDTATGGDKKVVLESG
                     AVVSTPLFINVGDTLRINTDTGEYVERVEKA"
     gene            5223..5804
                     /locus_tag="VEA36_00840"
     CDS             5223..5804
                     /locus_tag="VEA36_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYF28896.1"
                     /translation="MAFSRLADELKDASDEEVLRLSQANPDLFAVLVSRYEDAFLRKA
                     KSILYSKEDAEEVVQDTFTRIYVYADRYQPQEGASFSSWAYAILTRLAFTRYQKLRKH
                     RARNQELDPEAYERLGEEDGFLEDLSIRSEVLVALSRIPEAASRVLRLQFIEGKSQEE
                     IAHLEGSTVSAVKTRVHRAKKLFKDAIDTTNTP"
     gene            5801..6124
                     /locus_tag="VEA36_00845"
     CDS             5801..6124
                     /locus_tag="VEA36_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28897.1"
                     /translation="MTESAIHKNVMRRVRTIHAVRPLASTTALSAFLLLGGLWGIGTQ
                     VWVAQVYANMPSVADVGALARFAIAAFANTEFIVQALTLVVLAAAVWIVRDGVRSLRY
                     SLRTT"
     gene            complement(6153..7205)
                     /gene="recA"
                     /locus_tag="VEA36_00850"
     CDS             complement(6153..7205)
                     /gene="recA"
                     /locus_tag="VEA36_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HYF28898.1"
                     /translation="MPAKKSAKKSAKPAAPETEATPSDAKAKSIELAIKEIQTKFGDE
                     AIMTYGSGKIAAIPSIPTGSIGLDAALGIGGLPRGRIIEIFGPESSGKTTLALHVVAE
                     AQKKGGICAFVDAEHALDPQYARNIGVKLSELLISQPDTGEQALEIVESLVRANSIDV
                     IVVDSVAALTPKDEIEGDMGSAQMGKQARLMSQALRKLTAITAKSNTIIIFINQIRMQ
                     IGVMFGNPETTTGGMALKFYTSVRLDIRRIAQIKKGEEIVGGRVRVKVVKNKVAAPFK
                     TTEFDLLYNEGISREGELIALGEKYGILEKSGSSYKYGGETIGRGYDATRTFLRENKP
                     IGNEILKQIREKLAAE"
     gene            complement(7281..7604)
                     /locus_tag="VEA36_00855"
     CDS             complement(7281..7604)
                     /locus_tag="VEA36_00855"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28899.1"
                     /translation="MVRPAIGIALALLALYGLAKAYPLLSGPSLTITSPAPYASVPDG
                     FVAIEGTARRTETLTLNSAVLLIDEEGHFSTVLTLPPGDAILSLTARDRFGRSVRDVR
                     TVHVP"
     gene            complement(7607..>9044)
                     /locus_tag="VEA36_00860"
     CDS             complement(7607..>9044)
                     /locus_tag="VEA36_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HYF28900.1"
                     /translation="GKPGVGDIKANANIIKRTFQNFGISVEMDEVSVGPTVTRYAIKP
                     AEGVRLQKIVSLVTNLELALAAHPVRIEAPIPGKSLVGIEVPNASKATVGLGGVLAAP
                     EWGASKKSLLSAIGRDITGTPHYVNVSKMPHGLVAGATGSGKSVAIHALITSLLYRNG
                     PNQLRFIMVDPKRVELTLYNGIPHLLTPVITDPKKCIMSLKWAAKEMERRYTILENER
                     VRDIDSYHSSIYEPAKKKGARELPEAMPYIIIVIDELADIMQSFPRELEAAIVRLAQM
                     SRAVGIHLVLSTQRPSVNVITGLIKANVPTRMALQVASQIDSRTILDGPGAESLLGAG
                     DMLYLSADMQKPVRIQTAYISEQEVKKVVSYIKQHNAGSLGALDLGGGTDGSGHHEPN
                     DAISMMHDADDVDDDLYLQAKEAVEEAGRASTSYLQRKLRIGYSRAARLMDLLEERGV
                     IGPADGSRPREVINRGAVAAPTEAPDVF"
BASE COUNT         1734 a         2705 c         2876 g         1729 t
ORIGIN      
        1 gcatgacgct cggtctcctg gtggcggggc tctacgtcgt catcaaccag ctcgaggcgc
       61 acgtcattca cccgctcgtg gtgaacaagg tggtgggcat cccgcccctc ttggtcatcc
      121 tcgcgctcat cgtgggcggc acgctcgccg gcttcctcgg cgttctgctc tccgtcccgc
      181 ttgcggccgc ggcccgcgag ttcctcgcgg acgtagagcg cgggaagaaa gcgaaggcca
      241 cagtctccta gcatggcccg gtacatcacg acgaccttgc cgtacgtgaa tgccgatccg
      301 cacatcggct tcgcggtcga gttggtccag gcggattccc tggtgcggct gtggcgtgcg
      361 ggaggggaag cggtcttctt caatacgggc accgacgagc atggccagaa gatatatgag
      421 gcggcccaga aagcaggacg cgatgtccag gaatatgtcg atcactacgc ggcggagttc
      481 cggaagctca aggacgtgct ctccctcgac aacccgacgt tcattcgcac gaccgatgcg
      541 catcacaagc aggcggcggc ggagatgtgg cggcgctgcg aggctgcagg ggatatatac
      601 aagaaatcct acgaagggct ctactgcgtc ggctgcgaac tgtataagaa cgaaaaggat
      661 ctcaccgggg acgggcactg cctcgtccat ccgcacctga agcccgaact cgtttcggag
      721 gagaactact tcttccgatt ctcgaagtac caggatgcgc tcatcgaata cctgagccgc
      781 gccgacgtgg tacttcctga ttggcggcgt gaggaggcgc tcgcgttcgc gaagggcggg
      841 cttgaggatt tcagcatctc gcgcgagaag gcacggctct cctggggcat cccggttcca
      901 ggcgacgaga gccaggtcat gtacgtatgg ttcgatgccc tcaccgatta catatccacg
      961 ctcggttggc cggaggatgc agatggattc ttcgagaaat tctggcagaa cggacgcacg
     1021 ctccaaatgg cggggaagga ccaagtacga ttccagtccc tcatgtggca ggccatgctc
     1081 atgtccgccg gcgtgaagag cacagatcag gtgttctatc acggattcat caactcgggc
     1141 ggccagaaga tgagcaagtc cttggggaac gttatcagcc cctacgagct cgtcgagaag
     1201 tacggtaccg atgcgacccg ttacctcctt ctgcggcacg tccatccgac cgaggactcg
     1261 gacgtgacct gggagcggct cgatgagtgg tataccgcga acctcgtgaa cgggctcggg
     1321 aacctcgttg cgcgcgtaat gaagctcgcc gaggagcacc tgccgggccc ggtcatactt
     1381 accgagctcg acacctccac cgaattcgcg tttacggaga agcttgaggc gttcaagttc
     1441 aatgaggcca tggacctcat ctgggagcac atcgccaaag gggacgagta catgactacc
     1501 gaggagccgt acaagaaggt gaaggaggag gcgaccaagg tggaggccat cgccgtcatc
     1561 gagaagctcg tgcggcatct cgcaaagctc gcggcacata tagaggtcgt gatgccctca
     1621 accgctgagg tcatccgcgc ggcggtccgg gagaaccgga agccggagaa cctctttccg
     1681 aggcttgaca agtaattccc gttcaggtat ctttaccagt acagatcgag atgagctgtt
     1741 tacggcttat ctatcaggtt tcggcccggt agcattttta caactaagcc gtaaacaatt
     1801 catctcgcac ctcctacagc ccccgcaccc gcgggggctc gttcgtatat agtgatagct
     1861 atccatgaag tattttgacg cacactgtca tatccagttc gagaagtacg acggggaccg
     1921 cacagcgctc atcgagcagc ttgtgcagga gggagttggc gggctcgtgg tcgggacgga
     1981 cctggcctct tcgagggccg cgatcgagct cgtggaggat ctcgacggct tctgggccac
     2041 ggtagggctc catccgaatc atgtgaatga tgaaacgttc gatgatggta tatatcgcgg
     2101 gctcgctatg cacccgaagg tggtggcgat cggcgagtgc gggctcgata actaccgccc
     2161 cggcgacgtc gaagcggcga agcccctgca acgcgacctg ttccgcaggc aggtggagct
     2221 ttcgctcgaa gccgataagc ccctcatgat ccatggccgt cccgccaagg ggggcatgga
     2281 cgcgtacgag gacctgctcg ccgtactcgc tgaatacccg ggcgcgcgcg gcaatatcca
     2341 tttcttcgtc gggacgccgg ccatcgcgcg gcgattcatc gatctcggct tcaccctctc
     2401 cttcacggcg gtcatcacct tcgctcgcga ctacgacgac gtcatccgct ccatcccgct
     2461 cgattcgatc ctttccgaga ccgactcccc gtacgtcgcc ccggccgcac ggagagggca
     2521 gcgcaatgac ccgcggtccg ctaaagacgt ggtcgccgcg cttgcgcaga tacgcggcga
     2581 ggacgaggag gtcgtccggg cggcggtcct ccggaatgcg cggcgcgttt tcagcctgta
     2641 attgccgggc cgtacccacc gtggtacagt ctgcccgtta tgaacaagga catcgaacac
     2701 ggtaccgacg gtaccgagct tgagctcgga gagggatata acgagtcccg catctacgag
     2761 ctcggcttcc acatcgatcc tgaactaccc caggaagagg tgaagaaggt ttatcagggt
     2821 atccgcgaga cggccgagaa ggccggctca ttggtcgcgg aaggggagcc ccagcgcatc
     2881 ccgctcgcgt acaccatcag ccgcatggag ccctccgggc gccgcgactt caactcggcg
     2941 tacttcgcct gggtagccta cgaggcggac ggtcaggggc acgatgagat gctccggctc
     3001 gcgaaggagc atcccgccct ggtgcgctac ctggacgtga agacggataa ggaggcagcc
     3061 aagcacgcgg ccgagatgca cgagatctac gcgaagctcg gcgagaagga ggagggccag
     3121 acctccgaag aggaggtctc ggacgtcgag ctcgaggcag ccctgaagga cgccgagatc
     3181 taggaggtat actttcccta tgtacctcaa caaagtactc ctctacggca acctcacccg
     3241 ggatccggag ctgaaggcat tgccgtccgg gcagcaggtg gcatcgttcg ggatcgcgac
     3301 caaccggacc tttaccaata aggaagggca gaagcaggag cagaccgagt tccacaacat
     3361 cgtggccttc ggtcgcaccg ccgaagtcat gggccagtac ctgaagaagg gccgccccgt
     3421 cttcatcgaa ggcaggctcc agacccgctc ctgggaatcc gaggggaaga agaactaccg
     3481 caccgagatc gtcgtggata ccttccagtt cggtccgcag gcgggaggcg ccggaggcga
     3541 ccgtagcgcc tacgctgccg gtggcgccgc cgcaggggca gtagccgctc ccgaggctgc
     3601 cgcgtccgcg atcgagtacc ccgaggagga gatcaacccc gaagacattc ctttctagtt
     3661 aatccgttac catggcatat caagcagaac gagaagagat cgaaaacaag aagttcgtcg
     3721 actacaagga tgtcgcatac ctgcgccagt tcacgaaccc gcatgcgaag atcctgggcc
     3781 gcaagcgcac cggcatcccc gccgagaagc agcgccagat ctcgctggcg atcaagcggg
     3841 cccgctacat ggcgctcatg ccgtacctcg cggcgtaatc gataggttct cgacccaggc
     3901 aacggccgct aatgaggcgg ccgttaccct ttgtatccat tgccgaactt gctgcttcaa
     3961 tcctgaggcc gtatgctccg tgcaggaccg cacagacagg gggcgaagta ctatgagatt
     4021 gaaatcgagc catgcgtggt tgctggcgct tgcagccgta attccgattc tcagcgttca
     4081 gtacctccgg gtacagtccg ccgagaacca tttgaatgcg gcacagtggg ccgcccgatg
     4141 cgtgaaggca cctcgctggg ccgtggagga actcgcgacc aagttcccgc accacggcga
     4201 ggccgccgtt caggtcgcgg gcttcgcggg ctctggccgc aagggcatca aggtgagcgt
     4261 gaacgaagcc ggcacgccgg actgccagac cgattacgcg ccggactacg gccaggtgat
     4321 ctttgcggcg gatcggcagc ctatggccgc gtacctgacg aagccgggga gcaatggcat
     4381 cgtccacatg ttcgcgatca tggagctggc gccggaccca gagctgtcgg ggaagcggat
     4441 cttcgtcatc tttacggggc atttcggccc gccagggacc aagccgcagc ggaagatcca
     4501 gacctaggca cattccagca ctgaaagaaa aagggcgccc ttcgtgagaa gggcgccctc
     4561 gatcttttgt ctgctacgcc ttctcgaccc gctccacgta ctcgcccgtg tccgtgttga
     4621 tgcgaagcgt atcccctacg ttgatgaaaa gcggtgtcga gacgacggcg cccgactcga
     4681 gcaccacttt cttatcgccg ccggttgccg tatctcccct cacggcaggc ggagcctcct
     4741 ttaccttgag atccatcttg atcgggatct ttacggatac cggtttctcg ttgtacagca
     4801 ggacctcgac ggggctgttc tgcttgagcc acatgacctt gtccgcgacc gcgtcctcgg
     4861 ggaaggcgaa gcggttcttc gggttccctt cttcagtgaa ccagctctcg ccgcggttgg
     4921 tgtagagata ggtcgcctgc attttggata tatcagcctc aggcacggtc tccgactggt
     4981 ggaaggagat ctcgagcacc ttgccggaga cgaggtggcg gagtttggtc tggttcacgg
     5041 gcttacgctg ctgcatgcgg aagatgtgcg aggagagcac ctcgtacggc tcgctgttgt
     5101 agacgatgac ggacttcggg aggatctcgt tgtacgaaag gatggccatg gaaacgtgga
     5161 tggtgggggc ggggttataa aaaatgcccg gcaccgggat agggggtatg atacaggaca
     5221 tcatggcctt ttcaaggctt gcggacgagc tcaaggacgc ctcggacgag gaggttcttc
     5281 gcctgtcgca ggcgaacccc gaccttttcg cggtgctggt ctcccgatat gaggacgcct
     5341 tcctccggaa ggccaagtcc atcctctaca gcaaggagga cgccgaggaa gtggtgcagg
     5401 acaccttcac ccgcatctac gtctatgcgg accgctacca gccccaggag ggcgcctcct
     5461 tctcctcctg ggcctatgcc attctcaccc ggctcgcatt caccaggtac cagaagctta
     5521 ggaagcaccg ggcccgcaac caggagctcg accccgaggc ctacgagcgg ctcggggagg
     5581 aggacgggtt cctcgaggac ctctcgatcc ggagcgaggt gctggtcgcc ctctcgagga
     5641 tacccgaggc tgcatcccgc gtgctccggc tgcagtttat agaggggaag agccaggagg
     5701 agatcgcgca cctcgagggc tcgaccgtgt ccgccgtgaa gacccgggtg caccgcgcaa
     5761 agaaattatt caaagatgct atcgatacga cgaatacacc atgaccgaat cagccataca
     5821 taagaatgtg atgcgacgcg tccgcaccat ccatgcggta cgcccgcttg cgtccaccac
     5881 cgcgctctcg gcctttctgc tgcttggcgg cctctggggc attgggaccc aggtgtgggt
     5941 ggcgcaggtg tacgccaaca tgccgtcggt tgcggacgtc ggtgcgctcg cacgttttgc
     6001 gatcgcggcg ttcgcgaaca ccgagttcat cgtgcaggcg ctcacactcg tagtgctcgc
     6061 ggctgcggtg tggatcgtgc gcgacggggt acgcagcctg cggtactcgc tccgcaccac
     6121 ctaacaaaag agcccctcac ggggctcttt tgttactccg ctgcaagctt ctcgcggatc
     6181 tgcttcagga tctcgttccc gatcggcttg ttctcccgca ggaaggtgcg ggtcgcatcg
     6241 tacccgcgac cgatggtctc tccgccgtat ttatagctcg aacccgattt ctcgaggatg
     6301 ccgtacttct cgccgagcgc gatgagttcg ccctcacggc tgatgccctc gttgtagagg
     6361 aggtcgaact cggtggtctt gaagggagcg gcgaccttgt tcttcaccac cttcacacgc
     6421 acccggccgc caacgatctc ctcgcccttc ttgatctgcg cgatgcgccg gatatcgagg
     6481 cgcaccgagg tgtagaactt caaggccatg ccgccggtcg tggtctccgg gttcccgaac
     6541 atgaccccga tctgcatgcg gatctggttg atgaagatga tgatggtatt ggactttgca
     6601 gtgatggcgg tgagcttgcg tagcgcctgg gacataaggc gagcctgctt gcccatctgc
     6661 gccgagccca tgtcgccctc gatctcgtcc ttgggagtga gtgcggcgac cgagtccacg
     6721 acgatgacgt cgatggagtt cgcacggacc agcgactcta cgatctcgag cgcctgctcg
     6781 ccggtgtccg gctgcgagat gaggagctcg gagagcttca ccccgatgtt cctggcgtac
     6841 tgcggatcga gcgcgtgctc cgcgtccacg aaggcgcaga tgccgccctt cttctgggct
     6901 tctgcaacga cgtggagcgc aagcgtcgtc ttaccggaag actcgggacc gaagatctcg
     6961 atgatgcgac cgcggggaag gccgccgatc ccgagcgcgg catcaagccc gatggagccc
     7021 gtcggaatgg agggaatggc agcgatcttg cccgagccgt aggtcatgat ggcctcgtcg
     7081 ccgaacttgg tctggatctc tttgatcgca agttcgatgg acttcgcctt cgcatcggag
     7141 ggcgttgcct ctgtttcagg cgcggctggc ttagcggact tctttgctga tttctttgca
     7201 ggcataacgt acgagctcta actggtaagg gaaccggaga attctaggtc cgatgggtga
     7261 tgtaatcgtg accgagatat ctaaggaaca tgaaccgttc tcacgtcacg tacgcttcgg
     7321 ccgaagcggt cacgggcggt tagggatagt atagcgtccc ccgggggaag ggtcaggacg
     7381 gtcgagaaat ggccctcctc gtcgatcaac aggacggccg aattgagggt gagggtctcg
     7441 gtgcgccgcg cagtgccctc gatggctacg aagccgtccg ggacgctcgc gtagggggca
     7501 ggggaggtaa tggtgagcga gggacccgag aggagcggat aggcctttgc gaggccgtag
     7561 agggcgagca gggcgagcgc aatgccgatc gcaggccgga ccatggctag aacacgtccg
     7621 gcgcctccgt gggggccgct acggcgcccc ggttgatgac ctcgcggggc ctcgagccgt
     7681 cagcgggccc gatgaccccc ctctcctcga ggaggtccat gaggcgcgct gcgcgcgagt
     7741 acccgatccg gagcttgcgc tggaggtacg aggtcgaggc gcgcccagct tcctcgaccg
     7801 cctccttggc ctggaggtag aggtcgtcgt cgacgtcatc cgcgtcatgc atcatggaaa
     7861 tcgcgtcgtt gggctcgtgg tggcccgagc cgtcggtgcc gccgccaagg tcgagtgcgc
     7921 ccagagagcc cgcgttgtgc tgcttgatgt aggagacgac cttcttcacc tcctgctcgc
     7981 tgatgtatgc cgtctgaatg cggaccggtt tttgcatatc ggcggagagg tagagcatat
     8041 cgcctgcgcc aagcaaggac tcggcgcccg ggccgtcgag gatggtcctc gagtcaatct
     8101 ggcttgccac ctggagcgcc atgcgggtcg ggacgttcgc cttgatgagg ccggtgatga
     8161 cgttcaccga ggggcgctgg gtcgacagca cgaggtggat gccgaccgca cgcgacatct
     8221 gcgcgaggcg cacgatcgcg gcttcgagct cgcgcgggaa gctctgcatg atatcggcga
     8281 gctcgtcgat gacgatgatg atatagggca tggcctcagg gagttcccgc gcacccttct
     8341 tctttgcggg ttcgtatata gagctgtggt acgagtctat gtcccgtacg cgctcgttct
     8401 cgagtatggt atagcggcgc tccatctcct tggcagccca cttgagcgac atgatgcatt
     8461 tcttcgggtc cgtgattacc ggcgtcagga gatgcggaat gccgttgtag agcgtgagct
     8521 cgacccgctt cgggtccacc atgataaagc ggagctggtt cgggccgttg cgatagagga
     8581 gggaagtgat gagcgcgtgg atcgcgaccg actttccgga accggtcgcg ccagctacaa
     8641 gcccgtgcgg catcttcgag acgttcacgt agtgcggggt gccggtgatg tcgcggccga
     8701 tcgccgagag gagcgatttc ttcgaagcgc cccactcggg ggccgcgagc acgccaccaa
     8761 gacccacagt tgctttcgag gcgtttggca cctcgatacc gacgagggat ttgcccggga
     8821 tgggcgcctc aatacgcact ggatgcgctg ccaaggcgag ctcgaggttc gtaacgagcg
     8881 atacgatctt ctgcagcctg accccttctg ccggcttgat ggcgtagcgg gtcacggtag
     8941 ggccgaccga cacctcgtcc atctccactg agatgccgaa attctggaag gtccgtttga
     9001 tgatgttcgc gttcgctttg atatcgccca cgcccggttt ccct
//
LOCUS       DASPKG010000023         9639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1932892, whole genome shotgun sequence.
ACCESSION   DASPKG010000023 DASPKG010000000
VERSION     DASPKG010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932892"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VEA36_00865"
     CDS             complement(<1..714)
                     /locus_tag="VEA36_00865"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HYF28901.1"
                     /translation="MASVCLYLHIHQPYRVKRYGLFDVGNDPEYFNHAGAGDLNNAFI
                     IRKVEEKSYLPTMDMLLLLLERHPEFKVSFSVSGVALEQFMEHAPAVIDRLKQLAATG
                     RVEFLGETYYHSLAFFYSKQEFERQVEMHRRLLRALFDVTPRVFRNTELAYTNELGVW
                     ADEAGYDGIITEGWEPVLGWRSPNYVYRPQGAKSTALLLKNYKLSDDVAFRFSSQDWA
                     EWPLTADKYAQWVHALHGSA"
     gene            complement(714..2132)
                     /locus_tag="VEA36_00870"
     CDS             complement(714..2132)
                     /locus_tag="VEA36_00870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYF28902.1"
                     /translation="MSASLLLTLLVTLAALATSLFFLFGAYQLLRGRSENIMVAVKRE
                     VARVAEEVQRVTEEVQQFTEELGPRLRMLMYGWEFPPFNSGGLGVACLGLAKGLAGRN
                     ADITFVLPKRLAIESPYARMAFANIPEVKAHAVNSPLSPYLNTDTYVKAADGSPLYGN
                     DIVSEALRYGVYGGRLARTEAHDVIYAHDWLSFPAGMEAKRVSGRPLVAHVHATQFDW
                     ASERGINQAIYDIEREGMHAADCVITVSERTKRTIIERYGVPAGKVAVVHNGIDEATA
                     PTETETERLGALKAAGYDIVLFLGRLTMQKGGDYFLEAAKRVLTTRPKTVFVVAGSGD
                     MERQLIEQAAALGIAGNVLFAGFLRGEDQYEAYRSADVFVMPSISEPFGITALEAMRI
                     GTPVILSKQSGVAEVAGSALQVDFWDTEKMAELIAQVLADPILRQRLIAEGKQVAEHM
                     TWDRAAAQVRSILDEVSPVRIP"
     gene            complement(2165..2611)
                     /locus_tag="VEA36_00875"
     CDS             complement(2165..2611)
                     /locus_tag="VEA36_00875"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HYF28903.1"
                     /translation="MSRLLTPAARRFLRYAAVGVTTLAFDLVLLAFVIEVGGVPYYTA
                     VPVCFAIAVSINYLVSRKHVFRGTARNFHTGYLYFLLAAAGGATLTTSGVALLIEGFG
                     MHYLPARLGVAGVVGICNYLLNLHFNFRVVGLHYSAPETVYNSGKN"
     gene            2659..3600
                     /locus_tag="VEA36_00880"
     CDS             2659..3600
                     /locus_tag="VEA36_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016855991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYF28904.1"
                     /translation="MAENIPAVRIDGLKKTYAASNKQPPVEALKGVSLTITQGEFLAL
                     LGPNGAGKTTMIGIIAGLVNKTEGKVEVQGVDATANPDKAKSFIGVVPQEMNFDIFEK
                     VFEIVVTQAGYYGISRTEAIPKAEQLLKDLGLWEKKDTQAQFLSGGMKRRLLIARALV
                     HEPKILLLDEPTAGVDVELRRGMWDFLRKLSSEGTTIVLTTHYLEEAELLADRVAIIN
                     HGIIIEEGTVKELITKLNYVTLLLDTVEPVKDATLVLLREMNVKKIDQDTLELTLSPG
                     QTVNEAIRKIAATGIQIANVRNAGSRLEEIFINLTKK"
     gene            3602..4378
                     /locus_tag="VEA36_00885"
     CDS             3602..4378
                     /locus_tag="VEA36_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019675957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYF28905.1"
                     /translation="MALRSPILVGYYTMVRKDVVRMLRIWGQTLLPPAVTMALYFAIF
                     GTFIGSQVAAIDGYPYIQFIVPGLVMMAVITNSYMNTVFSFYFAKWQKTFHEILVSPM
                     PSWAVVAGYVSGGVVRGFLTAIVVIAVSLFFTKLTVFSFAIIFGAAFLTSFLFSLLGL
                     INAMYAKSFDAINIVPTFVLTPLTYLGGIFYSIDQLPEFFRIISLFNPILYMVNAFRY
                     GFLGVSDVPLSLAFGVMGVMTVVAFGTVLYFFSKGSGMKQ"
     gene            complement(4768..5484)
                     /locus_tag="VEA36_00890"
     CDS             complement(4768..5484)
                     /locus_tag="VEA36_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28906.1"
                     /translation="MTTSQRALMRGIWAWSKGTERVKRTHTPWLVENLPWVPDENDTD
                     VYGRPRIRDHRVEFPETRKRIVPRWELYQRFRQSTEYLYWQAVSARELAQSYRQFNVG
                     CALLAFRPDRPHRQSYEVHVGMNLKVAQQARPVCAEPIAIGGAHGAGCSRAIGMVIVG
                     ELREEDVDVLKTLHPCTECRLLMEDHPIIRPDTIIVTARPPVDGRVPEYEIRTFKRLL
                     EFHRLLELPRKPAAKDGDHR"
     gene            complement(5887..7008)
                     /locus_tag="VEA36_00895"
     CDS             complement(5887..7008)
                     /locus_tag="VEA36_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYF28907.1"
                     /translation="METIPSLKQCAAWAIGLTDKYRYNPFIRTAVHITVLQIVLAIIM
                     IAIFSWAIDYAQDRTLSSINEHVTDLATGSDPEDLSTAIREVRAHAYELVFFIILALN
                     ALFGYFMARFALRPTRHSIAAQKQFIGNIAHELRTPLAIIKTNTEVALFDPKLDPVVK
                     QTFEDTLHELDRISEIINNLLTFDTMTRPRRLEFEPIDVRQVAETVLRRHLALAKERG
                     IRLAMDSGGPAIIDGNQTALEQVLTNLVKNAINYTPQHKDGSVRIIIEPGEEGRTVVT
                     VADTGIGIAQKDLFHIFEPFYRGDTSRARGVGSGTSGLGLAIVNELVRLHKGSITIRS
                     ALGRGTAIKITFPPVSRTTVNRVLPSEQGGMHEVSLDFS"
     gene            complement(6950..7624)
                     /locus_tag="VEA36_00900"
     CDS             complement(6950..7624)
                     /locus_tag="VEA36_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYF28908.1"
                     /translation="MRILIVEDEAKLAEGLKRGLESKGFAVDWLEESEKARTRILLYR
                     NEYDLILLDLMLPGVGGAEITKEARAEGVTTPIIILTARDETEYKVELLNCGADDYIA
                     KPFSFEELVARINAVLRRPEQTVQTTLTVRDIVLDPGKRTVTKDGKDVSLTLKEYSLL
                     EFFMRHPNEALSRETIMDHVWSFDFPGFSNVLDVHMKNLRKKLDTYGDDPLFETVRGV
                     GYRLNG"
     gene            7965..>9639
                     /locus_tag="VEA36_00905"
     CDS             7965..>9639
                     /locus_tag="VEA36_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HYF28909.1"
                     /translation="MKQVKKRNGQIVEFESKKITEAMRKAFEATHTPIEVEKLEQMTK
                     GVLAQIETNFAETTPSVEHIQDYVEMTLMKEGYLAVAKHYIVYRYEHTKIREEKQQEV
                     LEKIEENALKIKTRSGREIPFSEDSVRKNLARFVKGYEDAIDVETIVSQLKYEIYEGM
                     TTDELHKALTMVVRSMIERDPAYSKVAARLLLHRMYGDVFGPDYKSDNLAEEHVRVFK
                     TNMERLVAEGNLDPRMAEFDLDKLARALNYENDQLFEYMGLEIMASRYCMEDPKTKKT
                     LETPSMLWMRIAMGLAIAEKKEDRERVALEFYDVLSNFYYTPGGRTLFQAGALKAQLS
                     NCFLNTVPDSLEGIFKSVSDNAQYLKWSGGTGTDWTRVRATGSFIKGTGVPSQGIVPF
                     LKIANDVNLAINRSGKRRGAGCVYLETWHLDIEDFLELRKNTGDERRRTHDINTANWI
                     PDLFMKRVRDGGDWTLFSPSDVPDLHDLYGAAFEKRYMEYESMAKNGALEYSKVVSAQ
                     DLWKKMIGMLFETGHPWITWKDPCNVRSPQDHAGVIHNSNLCTEITLNTN"
BASE COUNT         1869 a         2968 c         2998 g         1804 t
ORIGIN      
        1 cgccgagcca tggagcgcgt gcacccactg cgcgtacttg tccgccgtta atggccactc
       61 cgcccaatcc tgggacgaga agcggaaggc gacatcgtcc gagagcttgt agttcttgag
      121 gagaagcgcg gtgctcttcg cgccctgcgg ccggtacacg tagttcgggc tcctccatcc
      181 caagaccggc tcccacccct ccgtgatgat gccgtcgtat ccggcctcgt ccgcccagac
      241 cccgagctcg ttcgtgtacg cgagctcggt gttgcggaag acacgcgggg tcacatcgaa
      301 gagtgccctc aacagccttc gatgcatctc cacctgccgc tcgaactcct gcttggaata
      361 gaagaaggcg agtgaatggt agtaggtctc gccgaggaac tcgacgcgcc cggtcgccgc
      421 gagctgcttt aggcgatcga tgaccgcggg cgcatgctcc atgaactgct cgagcgccac
      481 ccccgagacc gagaacgaga ccttgaactc cgggtgccgt tcgagcagca gcagcagcat
      541 gtccatggtg gggaggtagg acttctcctc gaccttccgg atgatgaacg cgttattcag
      601 atctcccgcg cccgcatgat tgaaatactc cgggtcgttg ccgacgtcga agaggccgta
      661 ccgcttcacg cggtagggct ggtggatatg gagatagagg cagacggagg ccatcagggt
      721 atgcggacag ggctgacctc gtcgaggatg gagcgcacct gggcggccgc gcggtcccaa
      781 gtcatatgct cggcgacctg cttgccctcg gcgatgagcc gctgacggag gatgggatcg
      841 gcgagcacct gcgcaatcaa ctcagccatc ttttcggtat cccagaaatc gacctggagc
      901 gcgctgccgg caacctccgc gacgcccgac tgcttggaga ggatgacggg cgtcccgatc
      961 cgcatggcct cgagcgccgt gatcccgaaa ggttcggata tggacggcat gacgaatacg
     1021 tccgccgacc ggtacgcctc gtactggtcc tcgccgcgca ggaacccagc gaagagcaca
     1081 ttgcccgcga tcccgagcgc ggccgcctgc tcgatcaact ggcgctccat gtctccggac
     1141 cccgcgacga cgaagaccgt cttcgggcgg gtcgtgagca cccgcttggc ggcctcgagg
     1201 aagtagtcgc cgcccttctg catggtgagg cggccgagga agagcacgat gtcgtaccca
     1261 gccgccttca gcgccccgag ccgctcggtc tcggtctcgg tcggggcggt cgcctcgtcg
     1321 atgccgttat gcaccaccgc gaccttgcca gcgggcacgc cgtagcgctc gatgatggtg
     1381 cgcttcgtgc gctcggagac cgtgatgacg cagtcggccg catgcatgcc ctcgcgctcg
     1441 atgtcgtaga tcgcctggtt gatgccgcgc tcgctcgccc agtcgaactg ggtggcgtgc
     1501 acgtgcgcga ccaagggccg gcccgacacg cgcttggcct ccatgcccgc cgggaaggag
     1561 agccagtcat gcgcgtagat gacgtcgtgc gcctcggtcc gtgcgagcct accgccgtag
     1621 accccgtagc ggagcgcctc gcttacgatg tcgttgccgt acaacggact cccgtccgcg
     1681 gccttcacgt aggtatcagt attcaaatag ggcgagagcg gggaattcac cgcatgcgcc
     1741 ttcacctcgg ggatgttcgc gaaggccatc cgcgcgtacg ggctctcgat cgcaaggcgc
     1801 ttgggcaaga cgaaggtgat gtccgcgttt cggccggcaa gccccttggc gagccccagg
     1861 caggcaaccc cgagcccgcc cgagttgaag ggcgggaatt cccagccgta catgagcatg
     1921 cgcagccggg gcccgagctc ctcggtgaat tgctgtacct cttcggtcac ccgctgcact
     1981 tcctcggcaa cccgcgcgac ctcgcgcttc accgcgacca tgatgttctc gctccggccc
     2041 cgcagcagct ggtacgcccc gaagaggaag aacagcgacg tcgcgagcgc ggcgagcgta
     2101 accaggaggg taaggagaag agatgccgac atagcggtac taccgccagt atacggcagg
     2161 aacgctagtt ttttccggag ttatacactg tttccggagc ggaatagtgg agccccacca
     2221 cccggaagtt gaagtgcagg ttgaggaggt agttgcagat gccgacgacg cccgcgaccc
     2281 cgaggcgcgc cgggaggtag tgcatgccga agccctcgat gaggagcgcg accccgctcg
     2341 tggtcagggt cgcgccgcct gcggccgcaa ggaggaaata aaggtagccc gtatggaaat
     2401 tgcgcgcggt gccccggaat acatgcttgc ggctcacgag gtaattgatc gacaccgcga
     2461 tcgcaaagca cacaggaacc gccgtgtagt aggggacgcc cccgacctcg atgacgaagg
     2521 ccagcaggac aagatcgaag gcgagcgtag taacgcccac cgccgcatac cggaggaagc
     2581 ggcgggcggc aggggtcagg aggcgcgaca tatgcacatc atagcctctt aatgcgcggg
     2641 tggtatactc gccctactat ggctgagaat atccctgcag tacggatcga tggcctcaag
     2701 aagacatacg cagcgagcaa caagcagcct ccggtggagg cgctgaaggg cgtctcgctc
     2761 accatcacgc agggggaatt cctcgccttg ctcggcccta acggcgcggg caagacgacc
     2821 atgatcggca tcatcgcagg gctcgtgaac aagacggagg ggaaggtcga ggtgcagggc
     2881 gtcgacgcga ccgcgaaccc ggacaaggcg aagtccttca tcggcgtggt gccccaggag
     2941 atgaacttcg acatcttcga gaaggtgttc gagatcgtcg tgacccaggc cgggtactac
     3001 ggcataagcc gaaccgaggc catcccgaag gccgagcagc tcctcaagga tctcgggctc
     3061 tgggagaaga aggacacgca ggcgcagttc ctgtcgggcg gcatgaagcg gcgcctcctc
     3121 atcgcgcgcg ccctggtgca cgagccgaag atcctgctgc ttgacgagcc caccgcaggc
     3181 gtggacgtcg agttgcgccg cggcatgtgg gacttcctcc ggaagctctc gtcggagggt
     3241 acgaccatcg tgctcaccac ccactatctc gaagaggcgg agctgctcgc cgaccgtgtc
     3301 gcgatcatca accacggcat catcatcgag gaggggaccg tgaaggagct catcacgaag
     3361 ctcaattacg tcacgctcct cttggatacc gtcgagccgg tcaaggacgc tacgctcgtg
     3421 ctcctgcgcg agatgaacgt gaagaagatc gaccaggata cgctcgaatt gacgctctcg
     3481 ccggggcaga ccgtgaacga ggccatccgg aagatcgccg cgaccggcat ccagatcgcg
     3541 aacgtccgga acgcgggcag ccggctcgag gagatattca ttaacctgac caagaaataa
     3601 catggcgctc cgctctccca tcctcgtcgg gtactacacc atggtccgca aggacgtggt
     3661 ccgcatgctg cgcatctggg ggcagaccct gttgccgcct gcggtcacga tggcgctcta
     3721 cttcgccatc ttcggtacct tcatcgggag ccaggtggcc gcgatagatg gctaccccta
     3781 catccagttc atcgtgccgg gcctcgtgat gatggccgtg atcacgaact cctacatgaa
     3841 cacggtcttc agcttctact tcgcgaagtg gcagaagacc ttccatgaga tcctggtctc
     3901 tccgatgccg agctgggcgg tggtcgccgg ctacgtctcg ggcggggtgg tgcggggctt
     3961 cctgaccgcg atcgtcgtca tcgccgtgtc gctcttcttc acgaagctca ccgtattcag
     4021 cttcgccatc atcttcgggg cggccttcct cacctccttc ctgttctcgc tcttggggct
     4081 catcaacgcg atgtacgcga aatcatttga tgcgatcaat attgtgccga ccttcgtctt
     4141 gaccccgttg acctacctcg gcggcatctt ctattccatc gaccagctgc cggaattctt
     4201 ccggatcatt tcgctcttca atcccatcct gtacatggtg aacgccttcc gctacggctt
     4261 cctcggggtc tccgacgttc cgctctcgct tgccttcggg gtgatgggcg tgatgaccgt
     4321 ggtcgcgttt ggcaccgtcc tgtacttctt cagcaaggga tcggggatga agcaatagtt
     4381 atccagatgg gctgcttgtg cccctacgga tgagtggtat cttcatcctg ccggagggga
     4441 ggtccccaaa ggtacgccgg gtagcctcac aggcgcccac aaccggagat tgagatatga
     4501 ctctcatcac caacgcggat ggccggctct tcaccgaggc catctcccgt cgccgcgaca
     4561 ccgccgaagc ccgtcagctg acgaccggct agggccagac gccggcctat aaggtggggc
     4621 tatcgggcgc gggatgaccg cgatgtgatc ttccagatcg ctaaggaacg ctgttgttcc
     4681 gcgcccgaag ccttgcgctt cttcgtacgt cacgccctgc gcgtggcgtt ttcatttggg
     4741 cgactaaagg aagtgaccat cgccgcgtca gcgatggtca ccgtctttgg ctgccggctt
     4801 ccgaggaagc tccagtaggc gatggaactc aagcaagcgc ttaaacgtcc ggatctcata
     4861 ctcggggacc cgtccgtcga ccggcggccg cgccgtcacg atgatcgtgt ccggccggat
     4921 gatcgggtgg tcctccatga ggagccggca ctcagtgcag gggtggagcg tcttgagaac
     4981 gtccacgtcc tcctcgcgca gctcaccaac aatcaccatg ccgatggccc ggctgcatcc
     5041 ggccccatgc gccccgccga ttgcgatcgg ttcggcgcat acgggacgtg cttgctgggc
     5101 gaccttgagg ttcatcccga cgtgcacctc gtacgactgc cggtgcggcc ggtccggtcg
     5161 gaaggcgagc agcgcgcagc ccacattgaa ttgccggtag gattgtgcga gctcacgggc
     5221 actgaccgcc tgccagtaca ggtactcggt ggactggcgg aagcgctggt agagctccca
     5281 gcgcggcacg atacgctttc gtgtctcggg gaactcgacc cggtggtccc ggatcctcgg
     5341 acgcccgtag acgtcggtat cgttctcatc gggcacccag ggaaggttct cgacgagcca
     5401 gggcgtatgg gtccgtttga cgcgctcggt tcccttactc catgcccaga tgccacgcat
     5461 cagggcgcgt tgactggtag tcattgccgt ctccgatgct ggtttccggg catactatgc
     5521 tccagcttct ataacttgtc atcccgtctc tcgggtacgc aaagggccgg gccggctccg
     5581 tactcgtacg gcgccggccc ggccactaag cgttcttctt ctcttcctga agcatatccg
     5641 ccagtattta gtgctggtaa cggatggtgg tgcggcggtg atcgtccgcc acaaattcgg
     5701 gcccaacagc aattccgtat ctaatcgttg cggtcatgca atcgtcggat gcataactgg
     5761 tgttgatccc gactcgaaag aactcatccc ccatttgagg tctttcccga cctcgtgccc
     5821 gtactatacg caggcgctta tgaaatcttt atgaactgct catcaggaaa cgctgagttc
     5881 tgtggattac gaaaagtcga gcgaaacctc gtgcatcccg ccctgctcgc tcgggagcac
     5941 gcggttgacg gtcgtgcggg acacaggcgg gaaggtgatc ttgatcgcgg tcccacgacc
     6001 cagcgcgctt cgaatggtaa tgctgccctt gtggaggcgc accagctcgt tgacgatcgc
     6061 aaggccgagt cccgaggtcc cggagcccac accgcgggcg cgtgaggtat cgccgcggta
     6121 gaagggctcg aagatgtgga agaggtcctt ctgggcaatg ccgatccccg tgtccgcgac
     6181 cgtgaccacg gtccggccct cctcgcccgg ctcgatgatg atgcgtacgg acccgtcctt
     6241 atgctgcggg gtgtagttga tagcgttctt gacgaggttg gtgagcacct gctcgagcgc
     6301 ggtctggttc ccgtcgatga tcgcgggccc gcccgagtcc atggcgagcc ggatgccgcg
     6361 ttccttcgcg agcgcgaggt gccggcggag cacggtctcc gcgacctgcc ggacatcgat
     6421 cggctcgaac tcgagccgcc ggggacgggt catggtatcg aaggtgagca ggttgttgat
     6481 gatctccgag atgcggtcga gctcatggag cgtatcctcg aaggtctgct tcacgaccgg
     6541 gtcgagcttg ggatcgaaga gcgcgacctc ggtattggtc ttgatgatcg ctagcggggt
     6601 ccgcagctcg tgcgcgatgt tcccaatgaa ctgcttctgg gccgcgatcg agtggcgggt
     6661 gggccggagc gcaaaacgag ccatgaagta cccgaacagg gcattgagcg ccaggatgat
     6721 gaagaagacg agctcgtagg catgcgcgcg gacctcccgg atggcggtcg aaaggtcttc
     6781 aggatcgctc ccggtcgcca gatcggtcac atgctcgttg atcgagctca gcgtgcggtc
     6841 ctgggcgtag tcgatggccc agctgaagat cgcgatcatg atgatcgcga gcacgatctg
     6901 gagcacggtg atatgcaccg ccgtgcggat gaatgggttg tagcggtact tatccgttaa
     6961 gccgatagcc cacgccgcgc actgtttcaa agaggggatc gtctccatag gtatcgagct
     7021 tcttgcgtag attcttcatg tgtacatcaa gaacgttcga gaagccaggg aagtcgaagc
     7081 tccacacgtg gtccatgatg gtctcgcggg acagggcctc gttcgggtgc cgcatgaaga
     7141 actccaagag ggagtactcc ttgagggtaa gcgagacgtc cttgccatcc ttggtcacgg
     7201 tccgcttgcc tggatcgagc acgatgtccc ggaccgtgag cgtggtctgg accgtctgtt
     7261 cggggcggcg cagcaccgca ttgatgcggg cgacgagctc ttcgaaggag aagggcttgg
     7321 cgatgtaatc gtcggccccg cagttgagga gctcgacctt gtattcggtc tcgtcgcggg
     7381 ccgtgaggat gatgatgggg gtcgtgacgc cctccgcccg ggcctccttg gtgatctccg
     7441 cgccccccac tccgggaagc atgaggtcga gcaggatgag gtcgtattcg ttgcggtaga
     7501 ggaggatgcg cgtccgggcc ttctcgctct cctcgagcca gtcgacggca aagcccttcg
     7561 attccaggcc gcgcttcagc ccctccgcca gtttcgcttc gtcttcgaca atcaggatac
     7621 gcatagcttg ggagtatcga gttactggat aagccttcaa gagagcagac gcacttcatg
     7681 aaggttactt acctcatagt atgaactgtt catgatgatt cgattaaggg tacctcgagg
     7741 ggagcgaaac ggacatgcta tcggacatgg ttgacaatca atgatgtccg atacgcaata
     7801 cctaccgcct gtacgatttc catggcgcta tgcacgctat ccccaaggcc agtttcgagc
     7861 ccttcgatgc taaaattcct ataacgagag acacgggtac acgggtgcgg cactacggtg
     7921 cgggctgctt actaaacggg aaatcataac gcaatacgtt gcatatgaag caggtcaaga
     7981 agcgtaatgg gcagatcgtc gagttcgagt cgaagaagat aaccgaggcg atgcgcaagg
     8041 ccttcgaggc gacgcatacg ccgatcgagg tcgagaagct cgagcagatg accaagggcg
     8101 tcctcgcgca gatcgagacc aatttcgccg aaaccacccc gtccgtcgag cacatccagg
     8161 actacgtcga gatgaccctc atgaaggagg ggtacctcgc cgtcgccaag cactacatcg
     8221 tctaccggta cgaacacacc aagatccgcg aggagaagca gcaggaggtg ctcgagaaga
     8281 tcgaggagaa cgccctcaag atcaagaccc gctcgggccg cgagatcccg ttctccgagg
     8341 atagcgtccg caagaacctc gcccgcttcg tgaaggggta cgaggacgcg atcgatgtcg
     8401 agacgatcgt cagccaactg aagtacgaga tctatgaggg catgacgacc gacgagctcc
     8461 ataaggcctt gactatggtc gtgcgctcga tgatcgagcg agaccccgcg tactcgaagg
     8521 tcgcggcgcg gcttctcctg catcgcatgt acggcgatgt gttcggcccc gattacaaga
     8581 gcgacaacct cgccgaggag catgtgcgcg tcttcaagac gaacatggag cgcctcgttg
     8641 cggaagggaa cctcgatccc cggatggccg aattcgatct cgataagctc gcccgggcgc
     8701 tcaactacga gaacgatcag ctcttcgagt acatgggcct cgagatcatg gcctcccgct
     8761 actgcatgga ggacccgaag actaagaaga ccctcgagac cccgtccatg ctctggatgc
     8821 gcatcgccat gggacttgcg atcgcggaga agaaggagga ccgcgagcgg gtcgcgctcg
     8881 agttctacga cgtcctctcg aacttctact acactccggg cggccgcacg ctcttccagg
     8941 cgggagcgct caaggcccag ctctccaact gcttcctcaa taccgtgccg gactccctcg
     9001 agggcatctt caagtccgtt tcggataacg cgcagtacct gaagtggtcc ggcggcaccg
     9061 gcaccgactg gacccgggtg cgggcgaccg gctccttcat caagggcacg ggcgtcccct
     9121 cgcagggcat cgtaccgttc ctgaagatcg ccaacgacgt gaacctcgcg atcaaccgct
     9181 ccgggaagcg ccgtggggca ggctgcgtct acctcgagac ctggcacctc gatatcgagg
     9241 acttcctcga gctccgcaag aatacgggcg atgagcgtcg ccgcacgcac gacatcaata
     9301 cggcgaactg gatccccgac ctctttatga agcgggtgcg cgacggggga gactggacgc
     9361 tcttctcgcc gagcgacgtc ccggacctcc acgacctcta tggcgcggcc ttcgagaagc
     9421 ggtacatgga atacgaatcg atggccaaga acggtgcgct cgagtactcc aaggtcgtgt
     9481 ccgcgcagga tctctggaag aagatgatcg gcatgctgtt cgagaccggg cacccctgga
     9541 tcacctggaa ggacccctgc aacgtccgtt caccgcagga ccatgccggc gtcatccaca
     9601 acagcaacct ctgcaccgag atcacgctga acaccaacg
//
LOCUS       DASPKG010000024         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_714226, whole genome shotgun sequence.
ACCESSION   DASPKG010000024 DASPKG010000000
VERSION     DASPKG010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714226"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VEA36_00910"
     CDS             complement(<1..369)
                     /locus_tag="VEA36_00910"
                     /inference="COORDINATES: protein motif:HMM:NF016382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HYF28910.1"
                     /translation="MHRFDVSPKEAVQLQLELKSQVKLVPLTKPITHIGGCDVSMNRF
                     AKEGFAGFIMLTNSLEPVAHSVVKDRIPFPYVPGLLSFREIPMLMKAWETLTIKPDVL
                     IVDGIGIAHPRRFGIASHLGV"
     gene            complement(373..457)
                     /locus_tag="VEA36_00915"
     tRNA            complement(373..457)
                     /locus_tag="VEA36_00915"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Ser,seq:tga)
     gene            509..907
                     /gene="ruvX"
                     /locus_tag="VEA36_00920"
     CDS             509..907
                     /gene="ruvX"
                     /locus_tag="VEA36_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HYF28911.1"
                     /translation="MRHLGIDFGTKKIGLALSDEAGRMGFPHAILPNDGRALDEVEKL
                     IEKEQVGAIVMGESMNFSGAENPVAGAARAFAEELAKRTGTPVHFEPEMLTTQEARRD
                     FEGVHVSRSGDVDASAAALILTSYLSRNHG"
     gene            900..1238
                     /locus_tag="VEA36_00925"
     CDS             900..1238
                     /locus_tag="VEA36_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28912.1"
                     /translation="MAKEQISMDAFAKIEVKVGTVLSAERVPETDKLLRLMVDLGEEA
                     PRQIVSGIAAYVPEPALLVGRQLSFVTNLAPRTIKGLESNGMLFAAGEGETFAFMVPD
                     HPVPPGTGTH"
     gene            1282..2370
                     /gene="pilM"
                     /locus_tag="VEA36_00930"
     CDS             1282..2370
                     /gene="pilM"
                     /locus_tag="VEA36_00930"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HYF28913.1"
                     /translation="MPGEHTLQRFTRVEFAAPRYLAPPAVGIDISASGIKLAVIKERL
                     HGLELVDYGDGRLELGTMNGGEIADRKAVIEAVRSLAKEHHVRYAYIALSESRSYLFE
                     ADVPGTTKAEWRTAVEAHIDEYVPLPPAEILFDITKTDQKPEATHVVGIAYATRLIEE
                     SLSVLDEAGIVATAVESETFSLPRALLPIGDTDTVLIIDLGRTTTKLVIVEKRVPRFA
                     TTLDIGGHALTLAVQKYFNVTEEEAKRVKAERGLVGGPGNEEYLAAMLSTVSVIREEI
                     ARRVSYWQTRTAESGHAPISRAILVGGNATVRGLPEYLEANLQLPVGLGDVFTNLASR
                     DHWLPPIDYMESLAYGTAIGLALRDYDG"
     gene            2360..>2739
                     /locus_tag="VEA36_00935"
     CDS             2360..>2739
                     /locus_tag="VEA36_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28914.1"
                     /translation="MTADLTNLLPRDRRRALGRDYLFRVFVVVFWLLTALAVIHGLLL
                     LPSYFYVNQQTQARQSQLATLELEASGASQQELKNRIKTLENRATLLAGLGTKATASS
                     ALRGVLAAPRPGVKLHGFTYTPPP"
BASE COUNT          501 a          905 c          870 g          463 t
ORIGIN      
        1 aacccccaag tgcgacgcga tcccgaaccg gcgcggatgc gcgatgccga tcccgtccac
       61 gatgagcaca tccggcttga tcgtgagcgt ctcccacgcc ttcatgagca tgggtatctc
      121 gcggaaggaa aggaggccgg ggacataggg gaaggggatg cgatccttca cgacgctatg
      181 cgcgacgggt tcgagagaat tagttagcat gatgaaaccg gcgaagccct ccttcgcgaa
      241 ccggttcatg gaaacatcgc agccgccgat gtgggtgatc ggtttggtga gcggcacgag
      301 ctttacctgg ctcttcagtt ccagctgaag ctggaccgct tccttcggtg agacatcgaa
      361 gcggtgcata ggcggagggg gtgggattcg aacccacgag gcctttcggc ctggatgttt
      421 tcaagacatc tcgtttcgac ctctcacgca cccctcccag ggaactcatt ctatagcacg
      481 gagcgcaggt ggcgtgtagg atgcagctat gcggcatctc ggcatcgatt tcggcacgaa
      541 gaagatcggg ctcgcgctct cggacgaggc gggcaggatg ggtttccccc acgccatcct
      601 cccaaacgac ggccgcgcgc tcgatgaagt cgagaagctc attgagaagg agcaggtcgg
      661 ggccatcgtg atgggcgaat cgatgaattt ctcgggggct gagaatccgg ttgcgggggc
      721 ggcccgggcg ttcgccgagg agcttgcgaa gcgtaccggc accccggtcc acttcgagcc
      781 cgagatgctc accacgcagg aggcacgccg ggattttgag ggcgtgcatg tatccaggag
      841 cggcgacgtt gacgcgtccg ccgccgccct catccttacc agctatcttt cccgcaacca
      901 tggctaagga gcagatttcc atggacgcgt tcgccaagat cgaagtgaag gtgggcaccg
      961 tgctctccgc ggagcgcgtg cctgaaacgg acaagctgct gcggctcatg gtggatctcg
     1021 gcgaggaagc gccgcggcag atcgtatcgg gcatcgcggc ctacgtgccg gagcccgcat
     1081 tactcgtcgg caggcagctc tccttcgtga cgaacctcgc gccgaggacc atcaagggcc
     1141 tcgaaagcaa cggcatgctc tttgccgcag gggaagggga gaccttcgcc ttcatggtcc
     1201 cggatcaccc ggtgccgccg ggcacgggaa cgcattagca gcgcgtattc gcgcatcggc
     1261 atgcgctata ctttttgcag catgcccgga gaacatacgt tgcaacgatt cacgcgcgtc
     1321 gagttcgcgg cgccgcgcta ccttgccccg ccggcggtcg ggatcgacat ctccgcgagc
     1381 ggcatcaagc tcgcggtcat caaggaacgc ctccacggcc ttgagcttgt tgactatggc
     1441 gacggccggc tcgagctcgg caccatgaac gggggcgaga ttgcggatcg caaggccgtc
     1501 atcgaggcgg tgcgctcgct cgcgaaggag caccacgtcc gctacgcata catcgcgctc
     1561 tcggaatcga ggagctacct cttcgaggcg gacgtgccgg ggacaaccaa ggctgagtgg
     1621 cggaccgcgg tcgaggcgca catcgatgag tacgtaccgc tgccgcccgc cgagatcctc
     1681 ttcgacatca cgaaaacgga ccagaagccc gaagcgacgc atgtggtcgg catcgcgtac
     1741 gccacccggc tcatcgagga gtcgctctcg gtgctggacg aagccggcat cgtcgcaacc
     1801 gcggtggaga gcgagacctt ctcattgccg cgtgcgctcc tgcccatcgg cgacaccgac
     1861 accgtgctca tcatcgacct cggccggacg accaccaagc tcgtgatcgt cgagaagcgg
     1921 gtaccgcgct tcgcgaccac gctcgatatc ggcgggcacg cgctcacgct cgccgtccag
     1981 aagtacttca acgtcaccga ggaggaagcg aagcgcgtga aggcggagcg tgggctcgtc
     2041 ggtggccccg gcaacgagga atacctcgcc gccatgctct ccaccgtctc cgtcatccgc
     2101 gaggagatcg cgcgccgcgt ctcgtactgg cagacccgca ccgccgagag cgggcatgcg
     2161 cccatctcgc gcgccatcct cgtcggcggc aacgccaccg tgcgcgggct tcccgaatac
     2221 ctcgaagcga acctccagct gccggtgggg ctcggcgacg tgttcacgaa cctcgcgtcc
     2281 cgtgaccatt ggctcccgcc gatcgattac atggaatcgc ttgcctacgg caccgcgatc
     2341 gggctcgcgc tccgcgacta tgacggctga cctcaccaat cttctcccgc gggatcgccg
     2401 ccgggcgctc ggccgggact acctcttccg cgtcttcgtg gtcgtgttct ggctcctcac
     2461 cgcgctcgcc gtcatccacg ggcttctcct cctgccgtcc tatttctacg tgaaccagca
     2521 gacgcaggcg cgccagtccc agctcgccac gctcgagctc gaggcgagcg gcgcgagcca
     2581 gcaggagctg aagaaccgca tcaagacgct cgagaaccgg gcaacgctgc ttgccgggct
     2641 tgggacgaag gcgaccgcaa gcagcgcgct gcggggtgtc cttgccgccc cgcgcccagg
     2701 cgtgaagctc cacggcttca cctatacccc acccccgaa
//
LOCUS       DASPKG010000025         6581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1663137, whole genome shotgun sequence.
ACCESSION   DASPKG010000025 DASPKG010000000
VERSION     DASPKG010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663137"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VEA36_00940"
     CDS             complement(<1..888)
                     /locus_tag="VEA36_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYF28915.1"
                     /translation="MASSKGSNKKGRPVKKAGGKAISKKRMPKKTAPKKSVKKAAKKT
                     APKAVKKAAPKKAAKKLPKKERAMAVVRAAVKGVKAKAAAEKQDKAELLIQKGKERGY
                     ITYSEILKEFPHVEDDIHFLDELYERFTVAGIDILEGGMLEDNADQYLATRNIKGRES
                     SGYDSIQIYLREIGQYPLLTASEERELAKRIEAGDAEARNILARSNLRLVVSIAKKYV
                     GRSPDLTLLDLIQEGNLGLFKAVDKFDFKKGFKFSTYATWWIRQAITRALADQSRTIR
                     IPVHMVETIAKYKQVSRRLA"
     gene            complement(945..2621)
                     /gene="dnaG"
                     /locus_tag="VEA36_00945"
     CDS             complement(945..2621)
                     /gene="dnaG"
                     /locus_tag="VEA36_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HYF28916.1"
                     /translation="MAADTVEQIKQKLTITDVVGQYVKLTRAGRYMRGLSPFNKEKTP
                     SFFVNPERGSYYCFSSSQGGDMFTFIEKMEGVDFKGALKILAEKAGVELVYQKGNQDK
                     GKLDRLREAMARAEAFYAAECGEGRSAAYRYAVERGLKQETIKEWNLGYAPDSWRALL
                     EKLSADGFTTQELIAAGLIKEADGKKGTWYDRFRNRLMFPIRDSAGRTVAFTGRALDP
                     NDQAKYLNSPETELYKKHEILFGMDKAKDAIRTRGFAVLVEGQMDLLMLHQIGFQNTV
                     ALSGTALSKEHLALMKRYADNLMLALDADRAGLAAAQKSAIAALGAGMRVKAVRMPLG
                     KDPADLAKDTPQDFTRRVSDAKPIVEFFLETLAATEKDPHRLILEAERVVLPLILAIQ
                     SPMEREHFVGVAARMLGGSPDAIRASLGKVRAAPAGNAGAEAAPEPLISKMKTSERLG
                     LQLRAVVESYPSSALALRVKDEYSRIIGVYPDEPVPERILFESGIAFGEAPGETAADD
                     LIRTFERAVLTEKLSDATGALRLAESANDTNAIQQAASKCRDIATRLASL"
     gene            2722..3033
                     /locus_tag="VEA36_00950"
     CDS             2722..3033
                     /locus_tag="VEA36_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28917.1"
                     /translation="MAIHPDLLSLIGGTASAVLLILTRFLYTFSVKKQDGVYLILAIL
                     TTFLGGACWAVTGLMLLFRGVNGADPEFALYGTLVLLLCIVSFEQGWWRTVNKQPMLT
                     D"
     gene            complement(3149..>6581)
                     /gene="rpoC"
                     /locus_tag="VEA36_00955"
     CDS             complement(3149..>6581)
                     /gene="rpoC"
                     /locus_tag="VEA36_00955"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HYF28918.1"
                     /translation="ERMGHIELVSPVAHIWFLRSMPSRMALLLGVPSADLEKVIYFAG
                     YIVTKVNEDVKKRMLAELENEYKAKHKATETDASRDALKEKMTQAKTEIDSVVVGRVF
                     DEMAYHRYSVRYGTLFEARIGAEAIYDIFRSMDLVALKAKLEERYPNAAAAEREKLAK
                     RIALISNMISAKVRPEWMFMTKIPVIPPALRPMVALEGGRHATSDVNDLYRRVINRNN
                     RLKKLLDIHAPEVILRNEKRILQEAVDALLDNSIRKSGANAGALTPAQRRPLKSLADY
                     LKGKQGYFRQNLLGKRVDYSGRSVIVVGPDLELDECGLPKHMALELFRPFVIAGLLDR
                     ELAFNIRGAGRLIEDGVPEVWEILEEAIQGKYVLLNRAPTLHRQGIQAFRPRLIEGDA
                     VQLHPLVCHAFNADFDGDQMAIHVPLSEEAQWEAREVMSANKNILKPGSGEMTVLAAK
                     PQDIVLGVYWLTKEVVGANGEGKYFASPNAAILAHEYGAVDLRAKVHILPTSDKVKYD
                     KFEGKPFETTVGRLLFNTVLPSDYPFVNEAVTKKVLARIMNECLVKYGLDRIPSIVNK
                     VKRFGFEYATKSGVTWSINDVPVPPEKIRVINEARQKVEKVEDDFRNGLLSEDEKRRM
                     VIEIWHGTKNAVEKLVEESIDPLGSVNDIIKSGARGTIGNLTQMTGMKGLIQNTAGET
                     IEVPITSSMIEGLNPVEYFMSTHGARKGLTDTALGTARAGYLTRRLFDVAQDSIVTEQ
                     DCGTKRGITIGRVSASGITIDFADAIRGRTLAAEAAGFKKGHYLSADDARTVAASDVA
                     SVQVRSPMSCETRFGICATCYGMDLSTQELVDLGEAVGTVAAQAIGEPGTQLTMRTFH
                     SGGTASVGGDITQGLPRVEEVLEKRSPKVPAVVAKSDGFVTEIKEDGRDKVIVIAVQA
                     GQEKKKGKNDGSSIEYAVTYPRMPLVKEGDSVARGQLITDGSADIDDLFEYAGRAMVQ
                     EYIIAETSKIYELQGASVSRKHLEVVVKQMFSFVKITDTGDSDYSVGDTLEQLEFEQE
                     VKRLEADNKLPPKAREMVLGIKASALSRRSFLSAASFEQTTKILIDAALKGSVDTLRG
                     LKENVILGRLIPAGTGFPGSKKAKAIDEMQARRFAAQRAADEARDAELAAAEANA"
BASE COUNT         1058 a         2093 c         2086 g         1344 t
ORIGIN      
        1 cgcgagccgg cgggacacct gcttatactt cgcgatcgtc tccaccatgt ggaccgggat
       61 gcggatcgtg cgggactggt ccgcaagcgc gcgggtgatc gcctggcgga tccaccacgt
      121 cgcgtaggtc gaaaacttga agcccttctt gaaatcgaac ttatccacgg ccttgaagag
      181 gccgaggttc ccttcctgga tgaggtcgag gagggtgagg tccggcgaac ggcccacgta
      241 cttcttcgcg atggagacca caaggcgcag gttcgagcgc gcaagaatat tgcgcgcctc
      301 ggcgtcccct gcttcgatgc gcttggcgag ttcgcgttcc tctgaggcgg tcaggagtgg
      361 atactgtccg atctccctga ggtagatctg gatggagtcg tagcccgagg actcccggcc
      421 cttgatgttg cgggtcgcca ggtactgatc ggcattgtct tcgagcatgc cgccctcgag
      481 gatgtcgatg cccgctaccg tgaagcgctc gtagagctcg tcgaggaagt ggatgtcgtc
      541 ctccacatgc gggaactcct tcaggatctc ggaatacgtg atgtacccgc gctccttgcc
      601 tttctggatg aggagctccg ccttgtcctg cttctcggcc gccgccttgg ccttcacgcc
      661 cttcaccgca gcgcgcacca ccgccatggc ccgctccttc ttcgggagct tcttggccgc
      721 cttcttgggt gcagccttct tcaccgcttt gggagcggtc ttcttggctg ccttctttac
      781 ggactttttg ggtgcggtct tcttcggcat gcgcttctta gaaatagcct tgcccccggc
      841 cttctttaca ggccttccct tcttgtttga tcctttagat gaagccatag ggaggatgaa
      901 taaaaagtta actaaggaaa atcgagcacg gatgactacg gaaactagag cgaggcgagc
      961 cgggtcgcga tgtccctgca cttgctggct gcctgctgta tcgcgttggt atcgtttgcg
     1021 ctctcggcca gccggagcgc tccggtcgcg tccgacaact tctcggtgag aaccgcacgc
     1081 tcgaacgtac ggataagatc atctgccgcg gtttctcccg gtgcctcgcc aaaggctatg
     1141 cccgactcaa aaaggatacg ttcggggaca ggctcgtccg ggtagacgcc aatgatacga
     1201 gaatactcat cttttacgcg taaggcaagg gctgagctgg gatagctctc gacgaccgcc
     1261 cgcagctgga ggccgagccg ctccgaggtc ttcatcttgg aaatcagggg ctccggagcc
     1321 gcctcagcgc cggcgttccc cgccggagcc gcacgcacct tcccgaggct cgcgcggatg
     1381 gcgtcggggc tcccgccgag catcctcgcc gcgaccccca cgaagtgttc ccgctccatc
     1441 gggctctgga tggcgaggat cagggggagc accacccgct cggcctcaag gatgagacga
     1501 tggggatcct tttccgttgc agcgagcgtc tcgaggaaga actcgacaat gggcttcgcg
     1561 tccgagaccc ggcgggtgaa gtcctgcggg gtatccttgg cgaggtccgc cgggtccttc
     1621 cccaagggca tccgcacagc cttcacgcgc atgccggcgc ccaaggcggc gatcgcggac
     1681 ttctgcgcgg ctgcaagccc cgcccggtcc gcatcaagcg ccagcatgag gttgtccgcg
     1741 taccgcttca tgagcgcgag gtgctccttg gagagcgcgg tcccggagag cgccaccgtg
     1801 ttctggaacc cgatctggtg gagcatgagg agatccattt gcccctcgac cagcactgcg
     1861 aacccgcggg tgcgtatcgc atccttggcc ttatccatcc cgaagaggat ctcgtgcttc
     1921 ttgtagagct cggtctcggg gctgttcagg tacttcgcct gatcgttcgg gtcgagcgcg
     1981 cggcccgtga acgccaccgt cctcccggca gaatcccgga tcgggaacat gagacggttg
     2041 cggaaccggt cgtaccaggt cccctttttc ccgtccgcct ccttgatgag cccggcggcg
     2101 atgagctcct gggtcgtgaa gccgtccgcg gacaatttct cgaggagcgc acgccaggag
     2161 tcgggtgcat agccaagatt ccattcttta atggtttcct gttttagacc gcgttcgacc
     2221 gcatagcgat atgcggcact tctgccttcg ccacattcag ctgcgtagaa cgcttcagca
     2281 cgagccattg cctcccgcag ccgatcgagc ttccccttat cctggtttcc cttctggtac
     2341 acgagctcaa cccccgcctt ctccgcgagg atcttcaggg cgcccttgaa gtccaccccc
     2401 tccatcttct cgatgaaggt gaacatgtcg cccccttggc tggaggagaa gcagtaatag
     2461 ctgccgcgct cggggttcac gaagaaggag ggggttttct ccttattgaa gggcgagagc
     2521 ccgcgcatgt accggcctgc ccgggtgagc ttcacatact gccccaccac gtccgtgatg
     2581 gtgagcttct gctttatctg ctcgacggta tcggcggcca tttcccacta tcttacccct
     2641 tttcgcgcag tcctgtttct tgacgcccct aagacccgtg ctacgttgcc ggtaggacca
     2701 acgccaacgg agaacggcac catggccatc cacccggacc ttctgagcct catcggcggg
     2761 acggcgagcg ccgtgctgct gatcctgacc cgattcctct acaccttctc ggtgaagaag
     2821 caggacgggg tgtacctgat cctcgccatc ctgaccacct tcctcggcgg agcctgctgg
     2881 gccgtcaccg gcctcatgct cctcttccgg ggcgtgaatg gcgcggaccc cgagttcgcg
     2941 ctctacggca ccctcgtcct gctcctctgc atcgtttcct tcgagcaggg atggtggcgt
     3001 accgtgaaca agcagccgat gctcactgac tgaactggcc tcccggcccc gccccttgta
     3061 accgccccgg tccccaccgg ggcggcagta cgtatagcac aacgcccgct ctctcgagag
     3121 cgggcgttgt tgcttgttaa acgttgtctt acgcgttcgc ttccgcagca gcaagctccg
     3181 catcgcgcgc ctcgtcggct gcgcgctgcg cggcaaaccg gcgcgcctgc atctcgtcga
     3241 tggccttcgc cttcttcgag cccgggaagc cggtgcccgc cgggatgagc cgaccgagga
     3301 tgacgttctc cttgaggccc cggagcgtgt ccaccgagcc cttcaaggcg gcatcgatga
     3361 ggatcttcgt ggtctgctcg aaggaggcgg ccgagaggaa ggagcggcgg gagagcgcgg
     3421 acgccttgat gccgagcacc atctcgcggg ccttcggcgg cagcttgttg tccgcctcaa
     3481 gccgcttcac ctcctgctcg aattcgagct gctcgagggt atcgccgacc gagtaatcgg
     3541 agtcgccggt gtccgtgatc ttcacgaagg agaacatctg cttcacgacc acctcgaggt
     3601 gcttgcggga cacgcttgcg ccctgcagct cgtagatctt cgaggtctcc gcgatgatgt
     3661 actcctggac catggcgcgg ccggcgtact cgaagaggtc gtcgatgtcc gccgagccgt
     3721 cggtgatgag ctggccgcgg gcgaccgaat ccccttcctt gacgagcggc atacgaggat
     3781 acgtgacggc gtactcgatg gaggagccgt cgttcttccc cttcttcttc tcctggcctg
     3841 cctggaccgc gatcacgatg accttgtcgc ggccatcctc cttgatctcg gtgacgaagc
     3901 cgtctgactt ggcgacgacc gcaggcacct tgggcgagcg cttctcgagg acttcctcga
     3961 cgcgcgggag gccctgggtg atatcgccgc ccacggaagc ggtaccgccc gagtggaagg
     4021 tacgcatggt gagctgggtg cccggctcgc cgatcgcctg cgcggcgacc gtacccacgg
     4081 cctcgccgag atcgacgagc tcctgggtcg agaggtccat gccgtagcag gtcgcgcaga
     4141 tgccgaaccg cgtctcgcag ctcatcgggg agcggacctg gacgctggcg acgtcgcttg
     4201 ccgctacggt gcgcgcgtca tccgcagata ggtagtgccc cttcttgaag ccagcggctt
     4261 ccgcagcgag cgtgcggccg cggatcgcat ccgcgaagtc gatcgtgatg ccgcttgccg
     4321 acacgcgccc gatcgtgatg ccccgcttcg tgccgcagtc ctgctccgtc acgatggagt
     4381 cctgagcgac gtcgaagagg cgacgggtga ggtagcccgc gcgcgcggtg ccaagcgcgg
     4441 tgtcggtgag gcccttgcgg gcgccgtgcg tactcatgaa gtactcgacg gggttgaggc
     4501 cctcgatcat ggacgaggtg atgggcacct cgatggtctc gccggcggtg ttctggatga
     4561 ggcccttcat gccggtcatc tgggtgaggt tgccgatcgt gccgcgggcg cccgacttga
     4621 tgatgtcgtt caccgagccc aagggatcga tcgattcctc gacgagcttc tccacggcat
     4681 tcttcgtgcc gtgccagatc tcgatgacca tgcggcgctt ctcgtcttcg gagaggaggc
     4741 cgttgcggaa atcgtcctcc accttctcga ccttctggcg cgcctcgttg atgacgcgga
     4801 tcttctcggg cggcaccggc acatcgttga tggaccaggt gacgccggac ttcgtggcgt
     4861 actcgaagcc gaagcgcttc accttgttca caatgctcgg gatgcggtcg aggccgtact
     4921 tcacgaggca ctcgttcatg atgcgggcga gcaccttctt cgtgaccgcc tcgttcacga
     4981 acggatagtc ggatggaagc acggtgttga agaggaggcg tccgacagtg gtctcaaacg
     5041 gcttcccttc gaacttgtcg tatttcacct tgtcggaggt cgggaggatg tggaccttgg
     5101 cgcgcaggtc caccgcgccg tattcatgcg cgaggatggc tgcgttcggg gaggcgaaat
     5161 acttcccttc gccgttagcg cccacgacct ccttcgtgag ccagtacacg ccgagcacga
     5221 tgtcctgggg ctttgccgca agcaccgtca tctcgccgga accgggcttg aggatgttct
     5281 tgttcgcgct catgacctcg cgcgcctccc attgcgcctc ctcggaaagg ggcacgtgga
     5341 tggccatctg gtcgccgtcg aagtccgcgt tgaatgcgtg gcagacaagc ggatggagct
     5401 gcacggcgtc gccttcgatg aggcgcgggc ggaaggcctg gatgccctgg cggtgcagcg
     5461 tcggcgcgcg gttcaagagc acgtacttgc cctggatggc ctcttcgagg atctcccaga
     5521 cctccggtac gccgtcctcg atgagacgac ctgcgccgcg gatgttgaag gcgagctcgc
     5581 ggtcgaggag cccggcaatg acgaacgggc ggaagagctc aagcgccatg tgcttcggga
     5641 gaccgcactc gtcgagctcg aggtcggggc ccaccacgat cacggagcgg ccggagtagt
     5701 ccacgcgctt gccgaggagg ttctggcgga agtagccctg cttgcccttg aggtagtccg
     5761 caagggactt caagggacgg cgctgcgcgg gcgtgagcgc gcctgcgttc gcgcccgact
     5821 tgcggatgct gttatccagg agcgcgtcca ccgcctcctg caggatgcgc ttctcgttgc
     5881 gcaggatcac ctcaggcgca tggatgtcga ggagcttctt gaggcggttg ttgcggttga
     5941 tgacgcggcg gtagaggtcg ttgacgtcgg acgttgcatg gcggccgccc tcaagcgcca
     6001 ccatcgggcg gagcgccggc ggaatgaccg ggatcttcgt catgaacatc cactcgggac
     6061 ggaccttggc ggagatcatg ttgctgatga gcgcgatgcg cttagcgagc ttctcgcgct
     6121 cggccgcagc cgcgttcggg taccgctcct cgagcttcgc tttgagcgcc acgaggtcca
     6181 tcgaacggaa gatgtcatag atggcttcgg cgccgatgcg ggcctcaaac agcgtgccgt
     6241 agcggaccga gtagcggtgg taggccatct cgtcgaagac gcggccgacg accaccgagt
     6301 cgatctcggt cttcgcctgc gtcatcttct ccttgagcgc atcgcgggat gcgtcggtct
     6361 cggtcgcctt gtgcttggcc ttgtattcgt tctcgagctc ggcgagcatg cgcttcttca
     6421 cgtcctcgtt caccttcgtg acgatgtagc ccgcgaagta gatgaccttc tcgaggtccg
     6481 cagagggaac ccccaagagg agcgccatgc ggctgggcat ggagcgcagg aaccagatgt
     6541 gcgcgaccgg ggacacgagc tcgatgtggc ccatgcgctc g
//
LOCUS       DASPKG010000026         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_43306, whole genome shotgun sequence.
ACCESSION   DASPKG010000026 DASPKG010000000
VERSION     DASPKG010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43306"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VEA36_00960"
     CDS             <1..259
                     /locus_tag="VEA36_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28919.1"
                     /translation="TCGGSRHSDPAGARYTTDMSSVEFHEEADFIVRPRPRKASFFTR
                     VIYKLKLAKDEAGAQRVMLVITGICFTLAALLFLNLAGAFQ"
     gene            261..809
                     /locus_tag="VEA36_00965"
     CDS             261..809
                     /locus_tag="VEA36_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HYF28920.1"
                     /translation="MPTRGFTLIELLMVISIIALMSAVVLSGLSSARSKAKDAAIKRQ
                     VMEMRTLMAREFSDNGSYAFLKTASYGPITPAIDCPTPGAGTNNLRGTYGQRFRDSCL
                     AIKQTLAGNCGTNNVCLQFDRPTGSANTGKFSIIAYLPDESRRAPGGADRWLCVSYKG
                     HTGVSATVSWNDNPACPFSAGF"
     gene            858..1571
                     /locus_tag="VEA36_00970"
     CDS             858..1571
                     /locus_tag="VEA36_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HYF28921.1"
                     /translation="MGAIIPAILPTSREDLESKLARLDGLVGAVQVDIVDGRYASPAS
                     WPYTERGGEAAREDFLPYLGHFHFEFDLMVENPGLVAGAWIDHGVNRLTLHADHTRDL
                     GAVIEKLQVTYGHDKGFAPDLLSLGLAVGVETDLALIEPHLDRADYVQFMGIRTIGHQ
                     GEPFDNRVVRRIVAFRKAHSDMPIQVDGGVNLHTAPVLLDAGVSRLIVGSDLWKAPSL
                     TDELAKFEALAEEYGIYEK"
     gene            1592..2449
                     /locus_tag="VEA36_00975"
     CDS             1592..2449
                     /locus_tag="VEA36_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HYF28922.1"
                     /translation="MSVTDVEVKAIEGKAAAIRETIIEMLVAAGSGHTAGPLGMADIF
                     ASFYFHILTHDPKNPTWEERDRLVLSNGHIVPVQYAALAHAGYYPVEECLTLRKFGSR
                     LQGHPEREKLPGLETTSGPLGSGLSQAAGMAYGLRMDGKKNRIYCLMSDGEQQEGNIW
                     EAVMFAGKYRLSNLTAVIDRNNIQIDGMTEDVMPLEPLKEKYEAFNWHVIEANGHNVE
                     EFIDAVETAKAIAEKPTLILAHTIPGKSVPEIEHDYRWHGKPPSKEEAARFMQALRSG
                     GGRVPNEYA"
     gene            2442..2825
                     /locus_tag="VEA36_00980"
     CDS             2442..2825
                     /locus_tag="VEA36_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28923.1"
                     /translation="MRKARGAIVRTILFLAGFFFVFNVHMLVLQVAGGIPDVMGPAGL
                     WERPYVYLAINFLPMLILPDSLPVFLEPAFLLRWLVGVGVLAGAIALFERTRLPRRRW
                     TLVAFYLLILPPALFLVLKALYDVF"
BASE COUNT          494 a          981 c          929 g          529 t
ORIGIN      
        1 cacctgcggc ggctcgcggc attctgaccc tgccggggcg cggtatacta cggatatgag
       61 cagcgttgaa ttccacgaag aggcggattt catcgtccgg ccgcgtcctc ggaaagcgtc
      121 cttcttcacg cgggtcatct acaagctcaa gctcgccaag gacgaggcgg gcgcgcagcg
      181 ggtgatgctc gtcatcacgg gcatctgctt cacgctcgcc gcgctcctct tcctcaacct
      241 tgcgggcgcc ttccaatagt atgccgaccc gcggcttcac cctcatcgag ctcctcatgg
      301 tcatcagcat catcgcgctg atgagcgcgg tcgtcctctc cggcctctca agcgcgcgca
      361 gcaaggcgaa ggacgcggcc atcaagcggc aggtgatgga gatgcggacc ctgatggcgc
      421 gcgaattctc ggataacggc agctacgcgt tcctcaagac cgcaagctac ggtcccatca
      481 cgcctgcgat cgattgccct actcccgggg ccggcaccaa caacctgcgc ggcacatacg
      541 ggcagcgatt ccgcgactca tgccttgcga tcaagcagac cctcgccggg aactgcggga
      601 ccaacaacgt gtgcctccag ttcgaccggc cgaccggctc cgctaatacg ggcaagttct
      661 cgatcatcgc gtacctgccg gacgagagcc gccgggcgcc gggcggtgcc gatcgttggc
      721 tgtgcgtcag ctacaaggga cataccggcg tcagcgcgac cgtgagctgg aacgacaacc
      781 ctgcctgccc gttctcggcc ggcttctaaa cgcacccgtc tcccgttcgg gcggcgggtg
      841 ggcggtatac ttctcatatg ggcgccatca ttcccgccat tctccctacg tcccgcgagg
      901 acctcgaatc caagctcgcg cggctcgacg gcctcgtcgg cgccgtccag gtggacatcg
      961 tcgatgggcg atacgccagt cccgcctcct ggccgtacac cgagcgggga ggggaggccg
     1021 cgcgcgagga cttcctcccg tacctcgggc acttccattt cgagttcgac ctcatggtcg
     1081 agaacccggg cctcgtcgcc ggtgcctgga tcgaccacgg cgtgaaccgg ctcaccctgc
     1141 atgccgacca tacgcgggac ctcggcgcgg tcatcgagaa gctccaggtg acctacgggc
     1201 acgataaggg attcgcgccg gacctcctct ccctgggcct tgcggtcggg gtcgagaccg
     1261 acctcgcgct catcgagccc cacctcgatc gggccgatta cgtgcagttc atgggcatcc
     1321 gcacgatcgg gcaccagggt gagccgttcg ataaccgggt ggtgcgtcgg atcgtcgcct
     1381 tccggaaggc ccattctgat atgcccatcc aagtcgatgg cggcgtgaat ctgcatacgg
     1441 cgccggtcct cctcgatgcc ggcgtctccc ggctcatcgt cgggtcagac ctctggaagg
     1501 cgcccagcct caccgacgaa ctcgcgaagt tcgaggcgct cgccgaggag tacggcatct
     1561 acgagaagta gcgcgtgcta tcttttgggt aatgagcgtg acggacgttg aggtgaaggc
     1621 catcgaaggg aaggccgcgg cgatacgcga gaccatcatc gagatgctgg tcgcggcggg
     1681 ttcggggcac acggcagggc cccttggcat ggcggacatc ttcgcctcct tctatttcca
     1741 tatcctgacg cacgatccta agaacccgac ctgggaggag cgggatcggt tggtcctgag
     1801 caacgggcat atcgtgccgg tccagtacgc cgcgctcgcg catgcggggt attacccggt
     1861 cgaggagtgc ctcacgctcc gcaaattcgg ctcgcggctc caggggcacc cggaacgcga
     1921 gaagctcccg ggccttgaaa cgacctcagg gcccttaggg agcgggctct cccaggccgc
     1981 cggcatggcg tacggactcc ggatggacgg gaagaagaac cgcatctact gcctcatgtc
     2041 agacggcgag cagcaggagg ggaacatctg ggaggcggtc atgttcgcgg ggaagtatcg
     2101 gctttcgaac ctcaccgccg tcatagaccg caacaacatc cagatcgacg ggatgaccga
     2161 ggacgtcatg ccgctcgagc ccctgaagga gaagtacgag gccttcaact ggcacgtgat
     2221 cgaggcgaac gggcacaacg tcgaggagtt catcgatgcg gtcgagacgg ccaaggcgat
     2281 cgccgagaag ccgaccctga tcctcgcgca caccatcccc ggcaagagcg tgccggagat
     2341 cgagcacgac taccgctggc acggcaagcc gccctcgaag gaggaggcgg cgcggttcat
     2401 gcaagcgctc aggagcggcg gcgggcgcgt cccgaacgag tatgcgtaag gcccgcggcg
     2461 ccatcgtgcg gacgatcctt tttcttgccg gattcttctt cgtattcaat gtgcatatgc
     2521 tcgtgctcca ggtcgcaggc gggatcccgg acgtcatggg tcccgcgggg ctgtgggagc
     2581 ggccgtacgt ctacctcgcg atcaacttct tgccgatgct catcctgccg gactccttgc
     2641 cggtcttcct cgagcctgcc ttcctgctgc gctggcttgt gggcgtcggc gtgctcgccg
     2701 gcgcgatcgc gctcttcgag cgcacccgac tgccgaggcg ccgctggacg ctcgtcgcgt
     2761 tctacctcct catcctgccg cccgcgctct tccttgtcct gaaggcgctc tacgatgtct
     2821 tctagcctca tgatgcggtt cgccccggtc gcgtggttcc tgctcttcct cgccccgctc
     2881 gggatcatgg tggtgctcga cggattctgc agcgggcggg agctctgccg ggc
//
LOCUS       DASPKG010000027         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_319652, whole genome shotgun sequence.
ACCESSION   DASPKG010000027 DASPKG010000000
VERSION     DASPKG010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319652"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VEA36_00985"
     CDS             complement(<1..441)
                     /locus_tag="VEA36_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28924.1"
                     /translation="MLTLYHAIPRNMSGTVLYPLNALKDVDSSLYEAHAAKYEGREHL
                     MEERIPLLDCRWNDVLFLTAVSPVRFRAAYESTGYVRERPFRAYAIDVATLDQSRLAA
                     LTEMELNKPVRYEPFDPARYRDYATIPEATLDYWRAQRACGEERP"
     gene            complement(498..1094)
                     /locus_tag="VEA36_00990"
     CDS             complement(498..1094)
                     /locus_tag="VEA36_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28925.1"
                     /translation="MDLSGQRKFLLTQRFAAFVNRYDFFLGTDGERGERIGWAEQKRF
                     NIKERITVWEGEGQETTLFAIQAEKVLDLHGKFFVEDGAGKQVGYLQKDFRSSLLSST
                     WNIHDAAGALLFTTTEKSELLALARRLSFLIPFVGEVIDLIPFQFVFLKDGVRVGEQV
                     KRFRLRDTYVIEVTEALDGVDRRLILALAIALDELQGR"
     gene            complement(1164..>2060)
                     /locus_tag="VEA36_00995"
     CDS             complement(1164..>2060)
                     /locus_tag="VEA36_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28926.1"
                     /translation="LLNGAGKDSILSAELLKAAGVPFDFFAFAPSVAHERVAALVGAR
                     TIRVARRRDPRANMTAMLHGVSAAYPSVSTFTFIAVLLAELLGYDELVFSNERSADIG
                     NLTYRGLSVNHQWCKSSEAERLINDYIQRYLTPDISTRSLLREYSELEIVRRFVRYPQ
                     YLYAVTSCNSYFCYSRLEQSFLRTPYWCKECPKCMFLFACFAAFLPKKEVVRIFGGDL
                     YARRRRLPLIRRILGIEGFKPLDCVGEPEEMILAMHYAARRGEYARTPAMRLFEKEFP
                     SGYGFDELAQRVLAGAQEPNAS"
BASE COUNT          375 a          643 c          702 g          340 t
ORIGIN      
        1 cggccgttcc tcgccgcagg cccgctgcgc ccgccagtag tcgagggtcg cctcagggat
       61 cgtcgcgtaa tcgcgatagc gcgcgggatc gaaaggctcg taccggaccg gcttgttgag
      121 ctccatctcg gtcaaggccg cgagccgcga ctgatcgagc gtcgccacgt ctatggcata
      181 cgcccggaac gggcgctcgc gcacgtaccc cgtggattcg taggctgccc ggaaccggac
      241 gggggacacg gcggtgagga acaggacatc gttccaccgg cagtcgagga gcgggatgcg
      301 ttcctccatg agatgctcgc gcccctcgta cttggcggcg tgcgcctcgt agagggagga
      361 gtcaacgtcc ttgagcgcgt tcagcgggta gaggaccgtt cccgacatgt tgcgggggat
      421 ggcgtgatag agcgtcagca tactgcgggc ctccaggcgg gttctggcgg aaccctacca
      481 taagcgtaca ttttcagcta acgcccctgc agctcatcga gcgcgatcgc gagcgcgagg
      541 atgagcctcc ggtcgacgcc gtcgagcgcc tcggtgactt cgatgacata ggtatcccgg
      601 agccggaagc gcttcacctg ctcaccgacc cgcaccccgt ccttaaggaa cacgaactgg
      661 aaggggatga ggtcgatgac ctcgcccacg aacgggatga ggaagctcag gcgccgggcg
      721 agcgcgagca gctcgctctt ctcggtcgtg gtgaatagca gcgcgcccgc tgcgtcatgg
      781 atgttccagg tgctcgagag aagcgaggac cggaaatcct tctgcaggta gccgacctgc
      841 ttgcctgccc cgtcctcgac gaagaacttg ccgtggaggt cgagcacctt ttccgcctgg
      901 atggcgaaga gggtggtctc ctggccctcg ccctcccaga cagtgatgcg ctccttgata
      961 ttaaagcgct tctgctccgc ccagccgatg cgctccccgc gctccccatc cgtgccgagg
     1021 aagaagtcat agcggttcac gaacgccgcg aagcgctgcg tgaggagaaa cttcctctgg
     1081 ccggacaggt ccatgcccga agtatacggt acggatgagc ctcgctgtgc agggtgcgcc
     1141 ggcacgtccc gcgcatcagc gacctaagaa gcgttaggct cctgcgcgcc cgcgagtacc
     1201 ctctgcgcga gctcgtcgaa cccgtagccg gaggggaact ccttctcgaa caggcgcatc
     1261 gcaggcgtgc gggcgtattc gccgcgccgc gccgcatagt gcatggcgag gatcatttcc
     1321 tcgggctcgc cgacgcagtc gagcggcttg aacccctcga tgccgaggat gcgcctgatg
     1381 agcggcaggc ggcgcctgcg tgcatagaga tcgccaccga agatccggac cacctccttc
     1441 ttggggagga atgccgcgaa gcaggcgaag aggaacatgc atttcgggca ctccttgcac
     1501 cagtagggcg tgcgcaggaa ggactgctcg aggcgggagt agcagaagta gctgttgcag
     1561 ctcgtgacgg cgtacaggta ctgcgggtag cgcacgaagc gccgcacgat ctcgagctcg
     1621 ctgtactccc gcaggagcga ccgcgtcgag atatccggcg tgaggtagcg ctggatatag
     1681 tcgttgatga gccgctccgc ctcggacgac ttgcaccatt gatggttcac ggagaggccg
     1741 cgataggtga ggttgccgat gtccgcgctg cgctcgttcg agaagacgag ctcgtcgtag
     1801 ccgaggagct ccgcgagcag gacagcgatg aaggtgaacg tggagacgga cgggtacgcg
     1861 gccgataccc catggagcat cgcggtcatg ttcgcgcgcg ggtcccggcg gcgggcgacc
     1921 cggatcgtgc gcgcgccgac gagcgccgcg acccgttcat gcgcgaccga gggcgcgaag
     1981 gcgaagaagt cgaacggcac gcccgccgcc ttcaggagtt ccgcggaaag gattgaatcc
     2041 ttgcccgcgc cgttcaggag
//
LOCUS       DASPKG010000028         4001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1809490, whole genome shotgun sequence.
ACCESSION   DASPKG010000028 DASPKG010000000
VERSION     DASPKG010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809490"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VEA36_01000"
     CDS             complement(<1..798)
                     /locus_tag="VEA36_01000"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HYF28927.1"
                     /translation="MGALKRAIEAILPRSVYRALVRPYHFIITVLAAAACGFPAKKLT
                     VILVTGTKGKSSVTEMLFTVLRGAGHSAAVAGTIRFAINDETRPNRYKMTMPGRGFIQ
                     RFLKEAVAKGAKYAVIEVTSEATLQYRHRFLYPDALVFTNLQKEHIESHGSFEKYAAA
                     KLTIGRELARSPKRPRAIIANRDNKHSAPFLALPVEEVIPFSYADVTDVAAGPGTVAF
                     TLEGTRFDLHLPGSFSVMNALAVAHAARFVGVPFTESARALAALERIP"
     gene            complement(800..1351)
                     /gene="ruvA"
                     /locus_tag="VEA36_01005"
     CDS             complement(800..1351)
                     /gene="ruvA"
                     /locus_tag="VEA36_01005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HYF28928.1"
                     /translation="MIREIRGTVSSVEPAGAVVDVAGWGVFVHVADATLLTVGSAVHL
                     KTHMALKQDGIDLYGFAAEEDRRFFEQLVSVPGVGPKTAMSILRKAPRPALEGAIGSR
                     DLEYLTRVIGLGKKAAEKLMVELSEKIGATAVHGDEDAEVFDTLVALGYTEREARKTL
                     SSLPPSVVGKEARLKAALSNAHR"
     gene            1430..1693
                     /gene="rpsT"
                     /locus_tag="VEA36_01010"
     CDS             1430..1693
                     /gene="rpsT"
                     /locus_tag="VEA36_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010887951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HYF28929.1"
                     /translation="MAITKSAKKAHRASLRKHVFNVRRKRALTDTTKTVRKLVATDTK
                     AAEASLAAAYKAIDKAAKTGVIKKNAASRKKSRLAKAIARAKA"
     gene            complement(1730..1801)
                     /locus_tag="VEA36_01015"
     tRNA            complement(1730..1801)
                     /locus_tag="VEA36_01015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1766..1768),aa:Arg,seq:ccg)
     gene            complement(1824..2396)
                     /locus_tag="VEA36_01020"
     CDS             complement(1824..2396)
                     /locus_tag="VEA36_01020"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HYF28930.1"
                     /translation="MKIILGSQSAGRRAVLSKMGYVFETISPDIDEKQIRFDDPKTLT
                     LALAHAKADALLPRVPEPALLITSDQVVHYEGGIREKPETLEEARMFLKSYADHPATT
                     VTAVVVANTVTGRRAEGVDIARTYFKPIPDAVIEQAIEDGKVLRCCGGFAIDEPNLAP
                     YIDHIDGDPDSVTGLPTELVKRLLSEVEKV"
     gene            complement(2396..2470)
                     /locus_tag="VEA36_01025"
     tRNA            complement(2396..2470)
                     /locus_tag="VEA36_01025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2435..2437),aa:Arg,seq:gcg)
     gene            complement(2442..3137)
                     /locus_tag="VEA36_01030"
     CDS             complement(2442..3137)
                     /locus_tag="VEA36_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28931.1"
                     /translation="MAARHSPETRERVLELGRLGKTYTQIRKEFDIPKSTLSVWFRNE
                     GQVRDRTKQLEHLKRARVLASATKTRQRMERLRKAAKSAELELAGIHASTSVRKALLA
                     MLYWAEGTKSDKMGGLTFTNTDPVLAEFFLGELRATFPIDEQRIRIRIHLHDYHVPAA
                     ALAYWSQLLSVPQSQFGKIYVKKRSTRKRFRENFQGICFIHYGDGMIRRELLALARLI
                     AEKSGSRRSMDRT"
     gene            complement(3197..3670)
                     /locus_tag="VEA36_01035"
     CDS             complement(3197..3670)
                     /locus_tag="VEA36_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28932.1"
                     /translation="MNRPPLYEWEGTEYSFEEKGADWYWAVGIIAVAAIIACILFNNL
                     ILALLVAAAALAISLQAAKHPRTHRFAIYDRGLAIDERLYLFEDMFSFSVLEYADETL
                     PPSLSIKTRHFLAPHLLIPIVGYDPVEIYGFFLEHVEEGRHDESVFDHLVELLRL"
     gene            complement(3687..>4001)
                     /locus_tag="VEA36_01040"
     CDS             complement(3687..>4001)
                     /locus_tag="VEA36_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HYF28933.1"
                     /translation="HLGVLLDIPTVGCAKSVLIGRYAEPGDEPGATTDLIDPKTGEVI
                     GAALRTKCGVKPVFVSPGHLITLREAVELVFSTVRKHRLPEPTRLAHNLVNEYRLEDL
                     SR"
BASE COUNT          816 a         1146 c         1235 g          804 t
ORIGIN      
        1 ggggatgcgc tcgagcgcgg cgagcgcccg tgcgctttcg gtaaacggca ccccgacgaa
       61 ccgcgcggcg tgtgcgaccg caagcgcgtt catgaccgag aagcttccag gaaggtggag
      121 atcgaagcgc gtgccctcga gggtgaacgc gacggtgcct ggacccgcgg cgacgtctgt
      181 aacgtccgcg tacgagaagg ggatgacctc ctcgacagga agcgcgagga agggggcgct
      241 gtgcttgttg tctcggttcg cgatgatggc gcgcgggcgc ttcgggcttc gtgcgagctc
      301 gcggccgatc gtaagtttcg cggccgcata cttctcgaag gagccgtggg actcgatgtg
      361 ctccttctgg aggttggtga acacgagcgc gtccgggtac aggaagcggt ggcggtattg
      421 gagcgtggct tcggacgtga cctcgatgac ggcgtatttg gcgcccttcg cgaccgcctc
      481 cttgaggaat cgctggatga acccgcggcc aggcatcgtc atcttatagc gattcgggcg
      541 cgtctcgtcg ttgatcgcga agcggatcgt gccggcgacc gcggccgaat gccccgcacc
      601 gcgcagcacc gtgaagagca tctcggtgac ggaggacttc cccttggtgc cggtgacaag
      661 gatgacggtg agcttcttcg cggggaagcc gcaggccgct gcggcgagca ccgtgatgat
      721 gaagtggtaa ggacgcacga gcgcgcggta gacgctacgg gggaggatgg cttcgatcgc
      781 gcgtttgagt gcgcccatac tagcggtgag cgttcgagag cgcggctttg agccgggcct
      841 ccttcccgac gaccgacggc ggcagcgacg agagcgtctt gcgggcttcg cgttcggtgt
      901 atccgagggc gaccaaggtg tcgaagacct cggcgtcctc atcaccatgc acggcggtcg
      961 ccccgatctt ctccgagagc tcgaccatga gcttctcggc ggccttcttc ccgagcccga
     1021 tgacgcgggt gaggtactcg aggtcccgtg agccaatcgc gccctcgagc gccggccgcg
     1081 gggccttccg gaggatggac atcgcggtct tggggcctac cccaggcacg gagacgagct
     1141 gctcgaagaa ccgccggtcc tcctcggccg caaagccgta caggtcgatg ccgtcctgct
     1201 tgagcgccat gtgcgtcttg aggtgcaccg cgctcccgac cgtaaggagg gtcgcatccg
     1261 ccacatgcac gaagacgccc cagccggcga cgtccaccac cgcgcccgca ggctccacag
     1321 acgagacggt gccgcgtatc tcccggatca tatgtagaga taataccgct ccggcaggcg
     1381 gcggcataat ccttgcgaat accagggggc tacggtagga tgcctccaca tggcaatcac
     1441 caaatcagcg aagaaggcac accgcgcgtc gctgcgcaag cacgtcttca acgtacgccg
     1501 caagcgggcg ctcaccgaca ccaccaagac ggtccgcaag ctcgtggcga ccgacaccaa
     1561 ggcggccgaa gcgtcccttg ccgccgccta caaggcgatc gacaaggcag cgaagaccgg
     1621 ggtcatcaag aagaacgcgg catcccgcaa gaagtcgcgg ctcgccaaag ccatcgcccg
     1681 cgcgaaggcc tagcgaaaag aaccccttcc ggggttcttt tcgtacgggt gctcccggca
     1741 ggaatcgaac ctgcattttc tccttcggag ggagatatcc tatccattga acgacgggag
     1801 cgtggggaaa gaatactaca aaactagacc ttttcgactt cggataggag acgcttcact
     1861 aattccgtag ggagcccggt cacgctatcc gggtcgccat caatgtgatc aatataggga
     1921 gcgagattcg gttcgtctat cgcaaagcct ccgcagcagc gaaggacctt accgtcctcg
     1981 atcgcttgtt cgattactgc atcgggaatc ggcttgaaat aggttcgagc tatatcaacg
     2041 ccttcagcac gccgaccggt cacggtattc gctaccacga ccgcagtaac cgtcgtggct
     2101 ggatgatccg cgtagctctt gaggaacatc cgcgcctctt cgagcgtttc aggcttctcg
     2161 cgtatgcctc cttcgtagtg gacgacctgg tcgctcgtaa tcagcaaggc tggctcagga
     2221 acgcgcggaa gtaacgcatc ggcttttgcg tgagcaagag cgagcgttaa ggtcttaggg
     2281 tcatcgaagc gtatttgctt ctcatcaatg tcgggagata ttgtctcgaa aacgtatccc
     2341 atctttgaga gaacggcccg tctccccgct gattgtgagc cgaggattat tttcatggtg
     2401 ctcccggatg gaatcgaacc atcattttaa gatccgcaat cttatgtcct atccattgaa
     2461 cgacgggagc ctgatttctc cgcgataaga cgggctagcg cgagcaattc tcttcgaatc
     2521 atcccatcgc catagtggat gaaacaaatt ccttgaaaat tctcacggaa ccgctttcgg
     2581 gtacttcgct ttttaacata tatcttccca aattgcgatt gaggcactga aagcaattgc
     2641 gaccaatacg caagtgcggc agcaggcaca tgataatcgt gcaggtgtat gcgaatacgg
     2701 attctctgct cgtcaatagg gaaggttgca cgtagctcgc cgagaaagaa ttccgcgagc
     2761 acaggatcgg tattcgtgaa agttaggcca cccattttgt cgcttttggt gccttcagcc
     2821 cagtagagca tggcaagcag cgctttacgg acgcttgttg aggcatggat tcccgccaat
     2881 tcgagctcgg ccgatttggc cgcctttcga agccgctcca tgcgctgacg tgttttagtc
     2941 gctgaagcca gcacgcgtgc ccgttttaga tgctctagct gttttgttcg gtcacgtact
     3001 tgaccttcat tcctaaacca taccgataag gtacttttgg gaatatcgaa ttccttccgg
     3061 atctgcgtgt aggttttacc caatctgccg agttcaagaa cacgctccct agtttctgga
     3121 ctgtggcgag ccgccatgtg tccatattat agaacacata gaaatctagg cacacctcta
     3181 ttaagtgaat atctggctag agccgcagga gctcgacgag gtgatcgaag acggattcgt
     3241 cgtggcggcc ctcctcgacg tgctcgagga agaagccata gatctcgacc gggtcgtagc
     3301 cgacgatggg gatgaggagg tgtggggcga ggaagtgcct cgtctttatg gaaagtgagg
     3361 gcggcagggt ctcgtccgcg tattccagca ctgagaaaga gaacatgtcc tcaaagaggt
     3421 agaggcgttc gtcgatcgcg agcccgcggt cgtagatggc gaagcggtgg gtgcgggggt
     3481 gcttagcggc ctgaagcgag atcgcaagtg ctgccgccgc gaccaagagc gcgagaatca
     3541 ggttattgaa cagaatgcag gcgatgatcg cggcgaccgc aatgatgccg accgcccagt
     3601 accagtcagc gcccttctcc tcgaaggaat actcggtacc ttcccattca tagagcggag
     3661 gacggttcat gcgtacctct atagtatcac cggctgaggt cctcgagtcg gtattcgttg
     3721 accagattgt gcgcaagccg cgtgggctcg gggagccggt gcttgcgaac cgtcgagaag
     3781 acgagctcga cggcctcgcg cagtgtaatg agatgcccgg gagatacgaa gaccggtttt
     3841 acgccgcact tggtgcgcag cgccgccccg atgacctcgc ccgtcttcgg gtctatgagg
     3901 tctgtggtcg cgccaggttc atcgcccggc tcggcgtatc tgccgatgag tacgctcttg
     3961 gcgcagccca cggtcgggat atcgaggaga acccccaagt g
//
LOCUS       DASPKG010000029         5350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1270209, whole genome shotgun sequence.
ACCESSION   DASPKG010000029 DASPKG010000000
VERSION     DASPKG010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270209"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..587)
                     /locus_tag="VEA36_01045"
     CDS             complement(9..587)
                     /locus_tag="VEA36_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018033870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HYF28934.1"
                     /translation="MPNPKEEKTYVMIKPDGVKKGLIGEVIKRFEQRDLKIVALEMFQ
                     PTTEQIDNHYPKDPAWITRLGQKTLSTYEKYGYDANADFGTTEAEKIGPEVRKWVIDY
                     MTSAPLVKMVVQGTHAIDMVRKIAGPTLPYMADMGTIRGDFSNDSPAVANAEKRAVAN
                     IVHASETPEEAEHEIKYWFGDSTIHNYQRHGE"
     gene            complement(646..1146)
                     /locus_tag="VEA36_01050"
     CDS             complement(646..1146)
                     /locus_tag="VEA36_01050"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYF28935.1"
                     /translation="MDPIPEFGIKRENEERRDGGCAVVLDPASGLYAVGERTDDGMLI
                     LFSGGVDEGEDMHEGILREVREESGLHDFGTTEKIAEALTHYYNRAKGVNRCAQATCL
                     LIVLNSTASVAPTLEAHERFSLVWRTPEEILENWKLHNHDQGLDHWMYFLTRGVARAR
                     ELGVAP"
     gene            complement(1150..1557)
                     /locus_tag="VEA36_01055"
     CDS             complement(1150..1557)
                     /locus_tag="VEA36_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYF28936.1"
                     /translation="MSDKLQTAGLVPYYRKDGEVYIFMQMRGADAKRWPNTWGFFGGA
                     LEEGETPLMAVMREVQEELSIEIPEPEFFRHYEGETTESNIFLLEVDEAFPERVAVRD
                     GKYGKFLALSTIESVELRDYYRRIMPDLAAHFA"
     gene            complement(1547..2326)
                     /gene="map"
                     /locus_tag="VEA36_01060"
     CDS             complement(1547..2326)
                     /gene="map"
                     /locus_tag="VEA36_01060"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HYF28937.1"
                     /translation="MIVRSPEERAAVIEAGRRLGAVLEEVGRKVAPGVSTLDLETLAT
                     ALIRAGGDAPAFAGYTPEGAGRPYPASLCVSVNDEVVHGIPNERPRILEEGDIVALDL
                     GLIHDGIVVDSAITVPVGKVSGEAERLMAATREALERAIEVARPGARVGDISHATGAA
                     FKGTGFAVVKALGGHGVGRAVHEEPYIANDGHPGTGPEIIAGMVLALEPIANAGKAGV
                     HLASDGYTYRTKDGSLSAHFEHTILIEEAGTVVVTRRPHER"
     gene            complement(2323..2907)
                     /locus_tag="VEA36_01065"
     CDS             complement(2323..2907)
                     /locus_tag="VEA36_01065"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HYF28938.1"
                     /translation="MPHTKPDIVLIIGRPGAGKGTQAKLLAKRLRWIRISSGDRIKEI
                     RDGNEPFSERVREMYDKGTLLPDWLADYLLESALLELEPHVGVVAEGFARTENQARHF
                     YEIVSWLGRKLQVLYLDVSEDEARRRMLERGKTENRPDSDDEDKITERFQKFTDQTEP
                     GLTFFRELGLVVEVNGEQDPEKVAEDIEEILRKA"
     gene            complement(2931..3500)
                     /locus_tag="VEA36_01070"
     CDS             complement(2931..3500)
                     /locus_tag="VEA36_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HYF28939.1"
                     /translation="MQKGKFIILMGAMGSGKSMLVKHAIAKYPQLVIPYSYTTRPRRP
                     DAVENDHYRFLSVEEFEEMIEKGAFLEWARFGDNYYGTLKSEVEQELAAGLVVLKEME
                     VQGVRQVRDLLPKHQLVTVFVDAGAWDELTERAKNRAPISDEELAKRRQRYEDEVTFI
                     PEADQVITNRAGMQEEAKAAFEKIIEAAL"
     gene            complement(3546..4811)
                     /gene="secY"
                     /locus_tag="VEA36_01075"
     CDS             complement(3546..4811)
                     /gene="secY"
                     /locus_tag="VEA36_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HYF28940.1"
                     /translation="MLDNALRKFKTIFTDPVIRNRLLFVVAAFAIFRFLASIPIPGVD
                     QVALASFLNNNQFLGLLNIFSGGGLSNLSIVMLGVGPYITASIIMQLATVISPKVKAM
                     YSEEGEQGRTRFMSISRTIAVPFAVMQGVAFLTLLESQGIVATLSSFEFAVNVILIAA
                     GSMLLMWIGELITEFGIGNGVSLIIFAGIVAALPAGLSQALFTATAANIPAYVGFAVL
                     GLLVIFAVVLVTEAERSIPIHYARQVRGNSGAPTYLPVRLLQAGVIPIIFALSLLLLP
                     QMVLAALAAVGLPGIVGATAAYNAFAANPWYYGAVYFVLVVMFTYFYTSITFEPKRVA
                     DNLQKTGAFIPGVRPGPETERHIGNIVSRITLPGALFLGAVAVIPFIVQGLTGITAVL
                     VGGTALLIAVNVVIDLIRKVDAQASLREY"
     gene            complement(4819..5265)
                     /gene="rplO"
                     /locus_tag="VEA36_01080"
     CDS             complement(4819..5265)
                     /gene="rplO"
                     /locus_tag="VEA36_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HYF28941.1"
                     /translation="MQLHTLAPKTPRITSARVGRGGKRGKTSGRGTKGQKARAGRKMR
                     PEMRDLIKKLPKLRGHGQNRARTVRNRVPYTPVNLALLESAFAAGDTVTPETLYKKGL
                     IRARGGKLAFVKLLGTGTLTKALTVSDCATSASAKKAIEAAGGSVA"
BASE COUNT          923 a         1744 c         1680 g         1003 t
ORIGIN      
        1 cgtagtgatt actcgccgtg gcgctggtag ttgtggatgg ttgagtcgcc gaaccagtat
       61 ttgatctcgt gctcggcctc ttcgggggtc tctgaggcgt gcacgatgtt cgcgaccgcg
      121 cgcttctcgg cgttagcgac cgccggcgaa tcgttcgaga aatcgccgcg gatggtgccc
      181 atgtccgcca tgtacggaag ggtcggcccc gcgatcttgc gcaccatgtc gatggcgtgc
      241 gtgccctgca ccaccatctt caccaagggc gccgaggtca tgtaatcgat gacccacttg
      301 cgcacttcgg ggccgatctt ctcggcctcg gtcgtcccga aatcggcgtt ggcgtcgtag
      361 ccgtacttct cgtaggtcga gagcgtcttc tgcccgagcc gggtgatcca ggccgggtcc
      421 ttcgggtagt ggttgtcgat ctgctcggtg gtcggctgga acatctcgag cgcgacgatc
      481 ttgaggtcgc gctgctcgaa gcgtttgatc acctcgccga tgaggccttt cttgaccccg
      541 tcgggcttga tcatcacgta ggtcttctct tctttcggat tgggcatggt tgtaataaaa
      601 aatcgccttg ggcgcttata taggcacact atacgtgaaa atcccctaag gcgccactcc
      661 gagctccctg gcacgcgcga ccccgcgcgt aaggaaatac atccagtggt caaggccctg
      721 atcgtggtta tgaagcttcc agttctcgag gatctcctcg ggcgtccgcc ataccaagga
      781 gaagcgctcg tgcgcctcga gcgtgggagc gacactcgcc gttgaattga gcacgatcag
      841 caggcaggtc gcctgcgcgc accggttcac gcccttcgcc cggttgtagt agtgggtcag
      901 cgcctcggcg atcttctcgg tggtcccgaa gtcgtggagt ccgctctcct cccgcacttc
      961 gcgcaggatc ccttcgtgca tgtcctcccc ctcatcgacg ccgcccgaga agaggatgag
     1021 catgccgtca tcggtgcgct ccccgaccgc atacagcccg ctcgcggggt cgagtacgac
     1081 cgcgcatccg ccgtcccggc gctcctcgtt ctcccgcttg atgccgaact cggggatagg
     1141 gtccataccc tacgcgaaat gcgcggccag gtccggcata atccgccggt agtaatcacg
     1201 aagctccaca gattcgatgg tgctgagcgc gaggaatttc ccgtacttac cgtcccgcac
     1261 cgccacgcgc tcaggaaatg cctcatcgac ctcgagcaag aaaatattcg actctgtggt
     1321 ctcaccttca tagtgccgga agaattccgg ctcgggtatt tcgatggaga gctcttcctg
     1381 tacctcgcgc atgaccgcca tgagcggcgt ctccccttcc tcaagcgccc caccgaagaa
     1441 cccccaggta ttcggccatc gcttcgcgtc cgccccgcgc atctgcataa aaatgtagac
     1501 ctcgccgtcc ttgcggtaat acggaacgag cccagcggtc tgcagcttat cgctcatggg
     1561 gccgcctcgt caccaccacc gtccccgcct cctctatcaa aatcgtatgc tcgaagtgcg
     1621 cggacaggga cccgtccttc gtccggtacg tgtacccgtc gctcgccaga tgcacgcccg
     1681 ccttccccgc attcgcgatg ggctcgagcg caagcaccat ccccgcaatg atctccggac
     1741 ccgtccctgg atgcccgtcg ttggcgatat acggctcctc atgcacggca cggcccaccc
     1801 catgccctcc aagcgccttc acgacggcaa agcccgttcc cttgaaggcg gcaccggtgg
     1861 cgtgcgagat atcgcccacg cgcgcgccgg gccgggccac ttctatcgcc cgctccaggg
     1921 cctccctcgt ggccgccatg aggcgctcgg cctcgcctga gaccttccct accggcaccg
     1981 tgatcgcgga gtccaccacg atgccgtcgt ggatgaggcc gagatcgagc gcgacgatgt
     2041 ccccttcctc aaggatgcgc ggtcgctcgt tcgggatgcc gtgcacgact tcgtcattta
     2101 cggagacgca cagcgacgcg gggtacggac gccctgcgcc ctcgggggta taacccgcaa
     2161 acgccggagc gtccccgccc gccctaatga gggcggtcgc caaggtctcg agatcgagcg
     2221 tcgagacgcc aggcgcaacc ttccttccca cctcctcgag gaccgccccg agcctccttc
     2281 ccgcttcgat cactgccgcc cgttcctccg gactccgtac gatcatgcct tcctcaggat
     2341 ctcttcgata tcctcggcga ccttctccgg gtcctgctcc ccgttcactt ccaccacaag
     2401 cccgagctcg cggaagaacg tgaggcccgg ctcggtctgg tccgtgaact tctggaaacg
     2461 ctccgtgatc ttatcctcat cgtccgaatc cgggcggttc tccgtcttcc cgcgctcgag
     2521 catgcggcgg cgcgcctcgt cttcggagac atcgaggtag aggacctgga gcttcctgcc
     2581 gagccacgac acgatctcgt agaaatggcg ggcctggttc tcggtccggg cgaacccttc
     2641 cgcgacgacg cccacgtgcg gctcgagctc aagcagcgcg ctctcgagga ggtagtccgc
     2701 gagccagtcc ggcagtaggg tgcccttgtc gtacatctcg cgcacgcgct cggagaaggg
     2761 ttcgttccca tcgcggatct ccttgatgcg gtcgcccgac gagatgcgaa tccagcggag
     2821 gcgcttggcg agcagcttcg cttgagttcc tttaccggca ccggggcggc cgatgatgag
     2881 cacgatatca ggtttggtat gcggcataga cgaccttgag tgtaccaaat ttagagcgcg
     2941 gcttcgatga tcttctcgaa cgcggccttc gcctcctcct gcatcccggc gcggttggtg
     3001 atcacctggt ccgcctccgg gatgaaggtg acctcgtcct cgtagcgctg tcggcgcttg
     3061 gcgagctctt cgtcggaaat gggcgcccgg ttcttggccc gttcggtaag ctcgtcccag
     3121 gccccggcat ccacgaatac cgtcacgagc tggtgcttcg ggaggagatc gcggacctgg
     3181 cgcaccccct gcacctccat ttccttgagc accacgagcc ccgcagccag ctcctgctcc
     3241 acctcggact tcagggtgcc gtaatagtta tcgccaaagc gcgcccattc aagaaaggcg
     3301 cccttctcga tcatctcctc gaactcctcg accgagagga accggtaatg gtcgttctcg
     3361 accgcatccg gacggcgcgg gcgcgtcgta taggaatacg ggatgacgag ctgcgggtac
     3421 ttcgcgatcg catgcttgac gagcatgctc ttgccgcttc ccatcgcgcc catcaggatg
     3481 atgaatttcc ctttttgcat gaaagtaagg tatcacggaa caagcggcct cgcggccgcc
     3541 tggttctagt attcccgcaa ggacgcctgc gcgtccacct tgcgtatgag gtcgatgacc
     3601 acgttgaccg cgatcaggag cgccgtaccg cccaccagca ccgccgtgat gccggtcagg
     3661 ccctgcacga tgaacgggat gaccgccacc gcgccaagga agagcgcccc cgggagcgtg
     3721 atgcggctca cgatgttccc gatgtgccgc tcggtctcgg ggccagggcg cacccccggg
     3781 atgaaggcgc ctgtcttctg gaggttgtcg gcgacgcgct tgggctcgaa ggtgatcgag
     3841 gtatagaagt aggtgaacat caccaccagc acgaagtaca ccgcgccgta gtaccaggga
     3901 ttggcggcaa acgcgttgta ggcggcggtc gcgccgacga tccccggaag gccgaccgca
     3961 gccagtgccg cgagcaccat ctgcgggagc agcaggagcg agagcgcgaa gatgatcggg
     4021 atcaccccgg cctgcagcag ccggactggg aggtaggtcg gggcgccgga gttgccgcgc
     4081 acctggcggg cgtagtggat ggggatggag cgctccgcct cggtcaccaa gaccaccgcg
     4141 aagatgacga ggaggccgag caccgcaaag cccacgtagg ccgggatgtt cgctgcggtc
     4201 gcggtgaaca gggcctggga aagcccggcc gggagtgccg ccacgatgcc cgcgaagatg
     4261 atgagggaga cgccattgcc gatgccgaac tcggtgatga gttccccgat ccacatgagg
     4321 agcatggagc cggccgcgat caggatcacg ttcaccgcga actcgaacga ggacaaggtc
     4381 gctacgatgc cctgggactc aaggagcgtg aggaaggcca cgccctgcat gaccgcgaac
     4441 gggaccgcga tcgtgcggct gatcgacatg aagcgggtcc gtccctgctc cccttcctcg
     4501 gagtacatgg ccttcacctt cggcgagatc acggtcgcga gctgcatgat gatggacgcc
     4561 gtgatgtagg gcccgacgcc gagcatcacg atggagaggt tggagagccc gccgcccgag
     4621 aagatgttca ggaggccgag gaactggttg ttattcaaaa aggacgcgag cgcgacctgg
     4681 tccacgcccg ggatcgggat ggaggcgagg aagcggaaga tcgcgaaggc cgcgaccacg
     4741 aagaggagcc ggttgcggat gaccggatcc gtgaagatgg tcttgaattt gcgcagcgcg
     4801 ttgtcgagca tgggagggtt aggcgaccga tccgcctgcc gcctcgatcg ccttcttggc
     4861 gcttgccgag gtcgcgcaat cggatacggt aagcgccttg gtaagggtac cagtccccaa
     4921 gagcttcacg aaggcgagct tgccgccgcg cgcccgaatg agccccttct tgtagagggt
     4981 ctccggggtg acggtgtcgc ccgcggcaaa cgccgactca agcagcgcga ggttcaccgg
     5041 agtgtacggc acgcggttgc gcaccgtgcg agcacggttc tggccgtgac cgcgcagctt
     5101 cgggagcttc ttgatgaggt cgcgcatctc agggcgcatc ttgcggccgg cacgggcctt
     5161 ctgacccttg gtgccgcggc cggaggtctt gccgcgcttg ccgccacggc cgacgcgggc
     5221 cgaggtgatg cggggagtct tgggagcgag ggtgtggagc tgcataaaag tagggttagc
     5281 gtgcggacac ctggcggagc gcctcgacgg tcgcccgcgc gatggtgagc ttgttcttcg
     5341 tgcgggtgag
//
LOCUS       DASPKG010000030         4120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1947291, whole genome shotgun sequence.
ACCESSION   DASPKG010000030 DASPKG010000000
VERSION     DASPKG010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947291"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VEA36_01085"
     CDS             <1..750
                     /locus_tag="VEA36_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28942.1"
                     /translation="GNLAGASFVILLLIVPFLAVAGGGLSIKGLVSKETLVFTLLVIL
                     IPALLLADGRVTMLEGGFAILAYCALAYAIHNRPEGRVTNVCMPEHGGRRELYFDIAK
                     ILVGAAAVFIASHFLVEESIAVAAYLGVPASLFGLLLLSIGTNIPEIVIAVRAVLARN
                     KDIAFGDYLGSAAANVPIFGALALFTGGFAVESSQFVGTALLMFVGFVLFYVFARTRA
                     ELSRPEGMVLLLFYGGFVLVQFVNLVRFAAT"
     gene            complement(747..2735)
                     /locus_tag="VEA36_01090"
     CDS             complement(747..2735)
                     /locus_tag="VEA36_01090"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYF28943.1"
                     /translation="MTLDLAIMIASVAINATLGLFVLRHRPQTASSRLFLIMIAVIIV
                     WSYANFRIDTSPTDAAALAWMKVFYAAAIGIIALFLEFAHRFPFSRRSVLTALLPFAR
                     AGALAIIAVTMLTDLVFVSATLGPHGLASLAFGPLYLPFSVAALVGIMGAITELVLKR
                     RKSLLPARDQISFVVFGWSTFLALVVIVGAILPFFIPVLVNLSKLIPLLSVAMVGATA
                     YTIVRHNFLDIRIVLKRSLVYLVTLALVLGVYGLLVTLMDAAHGPSIEAVYLSAGITL
                     LAGIAGFPVLERILSRLVDPIFFRDSYDYAAAIHALSTILYTHIELDDLIAASERRLA
                     EFLHAEFVRITLQSRAPDQPHGATSFLGCGTLSIPISLNDEEIGVIVVGKKRTGDPYE
                     RRDIQLLETFAYQAATAFSRARLYAKAREHAHELERTVEERTRELREAQANERRMLVD
                     LSHNLQTPLAVFQARLEQLAHTKLASEDAHSLAQPVQRLSGFIYDLLSLAKLESEKPD
                     VHEGIDLSGLVDEIAEELTVIAGSKGVAIETAIAPGLRITGNERLLREAILNVASNAL
                     KYLSETGERRISFALAPKGQMAHLAVADTGRGISGDDLPHVFERFYRSRDAAALPTGN
                     GIGLSITKRIVEQHGGSIDIESTTGAGTRVHIRLPLPD"
     gene            complement(2789..3415)
                     /locus_tag="VEA36_01095"
     CDS             complement(2789..3415)
                     /locus_tag="VEA36_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28944.1"
                     /translation="MSGERDYRPYYRIERPSFGSNGFEEHLALTYEVYRKHGYLEGES
                     CPADLIDPSDGLDADYRLLYSPEDSLDGTFRILPWSAAGFPFERVCPDVAIPGGAARF
                     CETTRLAGRSASGPRSPTAYIAFEAVIESMVYVRARGLAGLLSLASPALSGYINSAFE
                     SVYHPLGRARWYRGGYVIPTVLWLREFEHVLRIRNPGLLAYYRERGGF"
     gene            complement(3412..>4120)
                     /locus_tag="VEA36_01100"
     CDS             complement(3412..>4120)
                     /locus_tag="VEA36_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HYF28945.1"
                     /translation="SSRRADSIGESKALMTARLMRAITDDTTIEVYPQGLGPSTQERF
                     LAGCDIVVDEIEFWNIGACIGMHQAARPHGIPILNGLTVGFASYVHLFESASMPVEEL
                     LGMSLEEASALEQRLSRGRASPVERERVMEAVLDAFIPDLPRYFSGPDGEQRNDFILR
                     RLREEGKAPILATNPQCAAGFVANRTLLRMLAADARTAPRSIAVLPPTPGYAVFDAAT
                     FDVRIVESRPPPIRGSA"
BASE COUNT          701 a         1268 c         1456 g          695 t
ORIGIN      
        1 gggaaccttg cgggcgcttc gttcgtgatt ctcctcctca tcgtgccatt ccttgcggtt
       61 gcgggcggcg ggctttcgat caagggcctc gtctcgaagg agacgctcgt cttcacgctg
      121 ctcgtcatcc tcatcccggc gctcctcctt gccgatgggc gcgtcacgat gctcgagggt
      181 ggattcgcca tcctcgcgta ctgcgcgctc gcgtacgcga tccataaccg cccggagggg
      241 cgggtgacga acgtctgcat gccggagcat gggggacggc gagagctgta cttcgacatc
      301 gcgaagatcc tcgtcggcgc cgcggcggtc ttcatcgcct cacatttcct agtcgaggaa
      361 agcatcgcgg tcgcggcgta cctcggggtc ccggcctcct tgttcgggct cctcctgctg
      421 tcgatcggga cgaacatccc ggagatcgtg atcgcggtgc gtgccgtgct cgcgcgcaac
      481 aaggacatcg cgttcggcga ctatctcggg tccgcggccg ccaacgtccc catcttcggc
      541 gcgctcgctc tcttcacggg cggtttcgcg gtggagtcga gccagttcgt cgggaccgcg
      601 ctcctcatgt tcgtcggctt cgtcctcttt tacgtattcg cgcgtacccg cgccgagctc
      661 tcgcgccccg agggtatggt gctccttctc ttctatggcg ggttcgtgct cgtgcagttc
      721 gtgaacctgg tgcgctttgc ggctacctag tccgggagcg ggagccggat atgcacgcgg
      781 gtaccggctc ccgtcgtgct ctcgatatcg atcgaaccgc cgtgctgctc cacgatgcgc
      841 ttcgtgatgg agagcccgat gccgtttccg gtcgggagcg cggcggcgtc cctgcttcgg
      901 tagaagcgct cgaagacgtg gggaaggtcg tccccggaga tcccgcgtcc cgtgtccgcc
      961 accgcaagat gcgccatctg ccccttcggt gcgagtgcga atgagatcct gcgctcgccg
     1021 gtttcgctca gatacttgag cgcattgctc gcgacgttga ggatcgcttc gcgcagcagg
     1081 cgctcgttcc ccgtgatgcg cagccccggc gcgatcgcgg tctcgatcgc gacgcccttg
     1141 ctgcctgcga tcacggttag ctcctcggcg atctcgtcca cgagacccga gagatcgatg
     1201 ccttcgtgga cgtcgggctt ctcgctctcg agcttggcga gcgacagcag gtcgtagatg
     1261 aacccggaga ggcgctgcac gggctgcgcg agcgaatgag cgtcctcgct cgcaagcttc
     1321 gtatgcgcga gctgctcgag ccgcgcctgg aagaccgcga gcggggtctg gaggttgtgc
     1381 gagaggtcga cgagcatgcg gcgttcgtta gcctgtgctt cacggagctc ccgggtacgc
     1441 tcctcgacgg tccgctcgag ttcgtgcgcg tgctcccgcg ccttcgcgta gaggcgcgcg
     1501 cgggagaagg cggtcgccgc ctggtaggcg aaggtctcga ggagctggat gtcccggcgc
     1561 tcatagggat cgccggtccg cttcttcccg accacgatca cgccgatctc ctcgtcattg
     1621 aggctgatgg ggatggagag tgtgccgcag ccgaggaagg aagtcgcgcc atggggctgg
     1681 tccggggcgc gggactggag cgtgatccgg acgaattccg catgcaagaa ttcggcgagc
     1741 cgccgctcgg acgccgcgat caggtcatcg agctcgatat gcgtatagag aatggtgctg
     1801 agcgcatgga tggcggccgc gtagtcgtag ctgtcacgga agaaaatggg gtcgaccagc
     1861 cgcgagagga tgcgctcgag cacggggaac ccggcgattc cggcaagcag ggtgatgccg
     1921 gcgctcaggt acactgcctc gatcgagggg ccatgcgccg cgtccatcag cgtgacgagg
     1981 agcccgtaca ccccgaggac gagcgcgagc gttacgaggt acacgaggct gcgcttcagg
     2041 acgatgcgga tatcgaggaa gttatggcgg acgatggtgt acgcggtcgc gcccaccatg
     2101 gcgacggaaa ggagcgggat gagcttcgag aggttcacga ggacgggtat gaagaaggga
     2161 aggatcgcgc ccacaatgac cacgagcgcg aggaaggtgg accagccgaa gacgacgaag
     2221 gagatttggt cgcgcgcagg caagaggctc ttgcggcgct tgaggacaag ctcggtgatc
     2281 gcgcccatga tgccgacgag cgcggcgacg ctaaacggca ggtagagcgg gccgaacgca
     2341 agcgacgcga ggccgtgcgg gccgagggtc gcggagacga agaccaggtc cgtcagcatc
     2401 gtgaccgcga tgatcgcgag cgctcccgca cgcgcgaagg gcagcagcgc ggtgaggacc
     2461 gagcggcgcg agaagggaaa ccggtgcgcg aactcgagga agagcgcgat gatcccgatc
     2521 gcggccgcat agaagacctt catccaggcg agcgcggcgg catcggtcgg gctggtatcg
     2581 atacggaaat tcgcatagga ccagacaatg atgaccgcga tcataatgag gaagagccgc
     2641 gagctcgccg tctggggccg atgacggagc acgaagaggc cgagcgtcgc gttgatggct
     2701 acgcttgcga tcatgatcgc gaggtccagc gtcatgggat tcattgtaga ggatgggcgg
     2761 caatcccgaa ggtgccgccc atagtgggtt agaaaccgcc gcgctcgcgg taataggcga
     2821 ggaggccggg gttgcggatg cgcagcacat gctcgaactc gcgcagccac agcactgtcg
     2881 ggataacgta gccgccccgg taccagcgcg cacggccgag cggatggtac acggactcga
     2941 acgcgctgtt gatgtacccg ctgagggcgg gactcgcgag cgagaggaga ccggcgagcc
     3001 cgcgggcccg cacgtacacc atcgattcga tcaccgcttc aaatgcgatg tacgcggtcg
     3061 ggctgcgcgg gcctgaggcg ctccggccgg cgagccgggt cgtctcgcag aaccgggcgg
     3121 ccccgcccgg gatcgccacg tccgggcaga ctcgctcgaa gggaaacccc gccgcgctcc
     3181 aggggaggat gcggaaggtg ccgtcgagcg agtcctccgg ggagtacagg aggcggtagt
     3241 ccgcatcgag gccgtcgctg ggatcgatga ggtccgcggg gcacgattcg ccctcgaggt
     3301 acccatgctt ccggtacacc tcgtaggtga gcgcgaggtg ctcctcaaac ccgttcgaac
     3361 cgaacgaggg acgctcgatg cggtagtagg ggcgatagtc ccgctcgccg ctcatgcgct
     3421 gcctcgtatg ggaggcggcc gcgactcgac gatacggacg tcaaaggtcg cggcatcgaa
     3481 taccgcatag ccgggagtgg gcgggagcac cgcgatggac cgcggcgcgg tgcgtgcatc
     3541 ggccgcgagc atccgcagga gggtgcggtt ggccacgaag ccggccgcac actgcggatt
     3601 ggtcgcgagg atgggtgcct tcccctcctc ccgcaaccgc ctgaggatga agtcattccg
     3661 ctgctcgccg tcgggcccgc tgaagtagcg ggggaggtcc gggatgaagg catcgaggac
     3721 cgcctccatc actcgctccc gctctactgg ggaagcgcgg ccgcgcgaga gcctttgttc
     3781 aagggcagac gcctcctcga gcgacatccc gaggagctcc tcgaccggca tactcgccga
     3841 ctcgaagaga tgcacatagc ttgcgaagcc gacggtgagt ccattgagga tcgggatgcc
     3901 gtgcggacgt gcggcctgat gcatgccgat gcaggcgccg atgttccaga actcgatctc
     3961 gtccacgacg atgtcgcagc ccgcgaggaa ccgctcctgc gtgctcgggc cgagcccctg
     4021 cggatatacc tcgatggtcg tgtcgtcggt gatcgcccgc atcagccggg cggtcatgag
     4081 ggccttggac tcgccgatcg aatcggcgcg ccgcgaactg
//
LOCUS       DASPKG010000031         8430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_460466, whole genome shotgun sequence.
ACCESSION   DASPKG010000031 DASPKG010000000
VERSION     DASPKG010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8430)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460466"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VEA36_01105"
     CDS             complement(<1..516)
                     /locus_tag="VEA36_01105"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HYF28946.1"
                     /translation="MKVSRKWLQNYFDAELPSAEELADALTFHAVEIEEIEGDLLDVK
                     VLPDRAAYLLSHRGVALELSAILGIPLKADPLRTPLSHWPRADELSLSIDAEGKTLRK
                     MGALVKGVKVGPSPDWLREALEAVGQRSINNVVDATNYVTLNMGQPLHAFDAGKLDWH
                     DGKLEITVRPAR"
     gene            complement(518..1276)
                     /gene="pheS"
                     /locus_tag="VEA36_01110"
     CDS             complement(518..1276)
                     /gene="pheS"
                     /locus_tag="VEA36_01110"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HYF28947.1"
                     /translation="MAAVRDILAAYMDSGRLHPLSADIREIADIFGRMGFDVASGPEL
                     EDDWHNFTALNVPADHPARDMQDTFWTKEEPPRVPRTQTSSVQIRYMEEKMGQGIAPP
                     YRIIVPGKVYRNEATDATHEAQFYQNEGLVVGEGISLAHLKGTLDRFFKEYIGEKSQV
                     RFRPSFFPFTEPSVEVDVWFEAPGKEGKWLEVMGAGMVHPQVLRNVGIDPEKYQGYAF
                     GGGIERLIMVKHGVPDVRLFHAGDLRFSYGFDIS"
     gene            1308..2546
                     /locus_tag="VEA36_01115"
     CDS             1308..2546
                     /locus_tag="VEA36_01115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYF28948.1"
                     /translation="MSLEVLAYPFIFLGLYFEVFMLLTFLSAPARERRLRAASQATPT
                     VAIIVPCWNEESTIGGTVRSLLAMDYPKDKLQIVLVNDGSTDNTRVVMDSYRDHPQIT
                     VLHKENGGKHTAINAGIEVAKDAEIVGCLDADSFVAPDALREMIACFDHPRVMAATPA
                     MSVHEPKNFLEGMQNAEYIMGIALRHALSAVNGIYVTPGPFSLYRRSIIEELGGFRHA
                     HQAEDMEMALRIQKAGYEIENAPRARVYTKVPRTVPKLIKQRTRWTTGFLRNVTMDYR
                     GLVGNPKYGVLGLLVLPLGFVAIMGGIGLFVVAVVEIIRGLLRTFAVTAGIPLSYTLA
                     PRFSLEWFYAPITFVLVISVIVTAIAISFMYIGRHVSNTPASLSLSIIGYAFLYGLIA
                     PFWLLRSIIDVVTGYRRAWR"
     gene            2568..3260
                     /locus_tag="VEA36_01120"
     CDS             2568..3260
                     /locus_tag="VEA36_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28949.1"
                     /translation="MKPSSMPLPLRNALLALIITLVIIGTIVATINYLDRQRLAELDA
                     LQNQLATDTLSIETQFALLEEAPCEDLEEGTAFSQELGNLGDRLSRSEQRLGSDDPEV
                     EQLKKQYTLLQIRDYLLTKRLAETCDVEPVTALYFYSNEESCEDCDRAGYALSYLRET
                     YPTLRVYSFDYDLELGALQTLVAVEKVEPRFPAFVINGKRSYGFTELEEFETRFPKAL
                     FATTTATSTRSR"
     gene            complement(3262..3337)
                     /locus_tag="VEA36_01125"
     tRNA            complement(3262..3337)
                     /locus_tag="VEA36_01125"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3302..3304),aa:Thr,seq:tgt)
     gene            complement(3357..3770)
                     /locus_tag="VEA36_01130"
     CDS             complement(3357..3770)
                     /locus_tag="VEA36_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28950.1"
                     /translation="MSYLIAIIVSLILLVGFLTLTAFEASHGVRILGGVRRRLDRKVG
                     QASFILSHVDLGSFVRDSIRDGIERVLHDIAHTSLLLVRFIERVLTRFVRQLRGRKEE
                     LPERPRRTFREAVQHVRRTVRLRRISIQEKQEGGE"
     gene            complement(3789..4373)
                     /gene="tsf"
                     /locus_tag="VEA36_01135"
     CDS             complement(3789..4373)
                     /gene="tsf"
                     /locus_tag="VEA36_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HYF28951.1"
                     /translation="MEISIDVVKQLREATGLSVMQCRKALEEAAGDVEKAEVILRKRS
                     AEAADKKSDREIGAGAVGIYLHDKAIGAMVLLASETDFVAKNPEFEALARELAMQVAA
                     TNPQYLTSEEIREEEKAAALAIFKEEVKDKPAEMQEKILAGKMDSYFRDSVLLNQPYI
                     KDESKTVKQLIDEASQKFGERVLVSKFARFSARG"
     gene            complement(4425..5126)
                     /gene="rpsB"
                     /locus_tag="VEA36_01140"
     CDS             complement(4425..5126)
                     /gene="rpsB"
                     /locus_tag="VEA36_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HYF28952.1"
                     /translation="MSTSSTTGPGVDRLFEAGAHFAHVKSRRHPSMRSFILGNKGGVE
                     IFDLVKTDEQLAAAKAAIAALARDGKTVLFVGGKREASDAVAATGKRLAQPYVAGRWL
                     GGTISNFTEIKKRIDRLADLSAKRDTGELSKIYTKLERIRIDREIERLSERLAGITSL
                     AKRPDALVIVDTRHEAHATKEARAAGIPIIGIMSSDCDLKDAAYPIVVNDASRTAIDL
                     VLGELATAFEEGQRG"
     gene            complement(5163..6116)
                     /locus_tag="VEA36_01145"
     CDS             complement(5163..6116)
                     /locus_tag="VEA36_01145"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HYF28953.1"
                     /translation="MEYSPEFKANHKTAYLAEEYERLQKEAEESRAAAGNDPELALMA
                     EEDEARVRARQQDILKDIEKIIEKDKEEAAKPTALVLEFRAGAGGDEAALFAGELRDM
                     YMKYAESRGWKVRVIDDLTLEIAGTDAYDALRYETGVHRVQRVPVTEKSGRIHTSTAS
                     VAALPIRQKPVVEINPADIEMEFTRSGGAGGQNVNKVETAVRLIHKPTGVDVRSSSER
                     SQLANREKAMAILAAKLEAIAIEEEAKQHADLRSGQIGTGDRSEKIRTYNFPQDRVTD
                     HRLKQNFHNLPKIMLGGIDAIVTALAEAAAAGGVGPAEAGD"
     gene            complement(6178..6432)
                     /locus_tag="VEA36_01150"
     CDS             complement(6178..6432)
                     /locus_tag="VEA36_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020033839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HYF28954.1"
                     /translation="MKKDIHPADYREVMFEDIASGEVFIIGSTIRTTETGTYKGKEYP
                     KVTVEISSKSHPFYTNEDRTLDKTGRVERFKQRAAAAKKK"
     gene            complement(6478..6975)
                     /locus_tag="VEA36_01155"
     CDS             complement(6478..6975)
                     /locus_tag="VEA36_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28955.1"
                     /translation="MAPGTTITFTATPQNGASGPYTWYDGSSNIIGSGATLQRQFNSD
                     GSVRAEGGGVFSNYCSVDVVSASCQASGPLDITATPARIVAGTPTAISVSWTASGIDS
                     ACTVRILGPDTQIGSSAHNAASCSSSGGPVSGGSVSAQTTYRLQCGSLWKDAVVNVIP
                     RYEEF"
     gene            complement(7767..>8430)
                     /locus_tag="VEA36_01160"
     CDS             complement(7767..>8430)
                     /locus_tag="VEA36_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28956.1"
                     /translation="LRGAHFYTGAPTYGPQHIEGLQFVRNAAGDTLWLSGSRSPDGSR
                     VVFAISTSTQAIMPFVQAEKVTPSAYQVLMRPTEGSGVQSIAYGYAPAFLDDTHISYF
                     TDAGIAVMDTQTGEATLLFRYPAGTVLSSGVRYSPDRRTVLWTDAMTGESVAGSITPS
                     AYTPLSTFKDLASPVLGNAAVYELRRTHEGQELWKRTLDGSSSAKVLTLPASLSILTM
                     AL"
BASE COUNT         1407 a         2741 c         2669 g         1613 t
ORIGIN      
        1 tcgagccggc cggaccgtga tctcgagttt cccgtcatgc cagtcgagct tgcctgcatc
       61 gaaggcgtgg agcggctggc ccatgttcag cgtcacgtag ttggtcgcgt cgacgacgtt
      121 gttgatcgag cgctgcccga ccgcctcgag cgcctcgcgc agccaatcag gtgacggccc
      181 caccttcacg cccttcacga gggcgcccat cttccggagc gtcttccctt cggcatctat
      241 cgagagcgag agctcatccg cccgtggcca atgcgaaagc ggggtgcgaa gcggatccgc
      301 cttcagcggg atgccgagaa tcgcggagag ctcgagcgcc accccgcgat gcgacaggag
      361 gtacgccgca cggtccggca ataccttcac atcgagcagg tctccctcga tctcctcgat
      421 ctcgaccgca tggaaggtga gcgcatcagc gagctcctcc gcgctcggga gctcggcatc
      481 gaaatagttc tggagccact tccgggagac tttcatgcta ggagatatcg aacccgtacg
      541 agaatcggag gtccccggca tggaagagcc ggacatcggg caccccgtgc ttcaccatga
      601 tcagacgctc gatgccgccc ccgaaggcgt agccctggta tttctcagga tcgatgccga
      661 cgttccggag cacctgcgga tgcaccatgc ccgcacccat aacctcgagc cacttccctt
      721 ccttcccggg ggcctcgaac catacgtcca cctcgaccga gggctcggtg aacggaaaga
      781 aggagggacg gaagcgcacc tgcgatttct cgccgatata ttccttgaag aaacgatcga
      841 gcgtgccctt caggtgtgcg agtgaaatcc cctcgccgac cacgagcccc tcgttctggt
      901 agaactgcgc ttcgtgggtc gcatcggtgg cctcgttccg gtacaccttt cccgggacga
      961 tgatccggta cggcggcgct atcccctgtc ccatcttctc ttccatgtac cggatctgga
     1021 ccgaggaagt ctgggtgcgg ggcacccgag gcggctcctc cttcgtccag aaggtgtcct
     1081 gcatgtcgcg ggccggatga tccgcaggga cgttgagcgc cgtgaagtta tgccagtcgt
     1141 cctcgagctc gggacccgag gcgacatcga agcccatgcg gccgaagata tccgcgatct
     1201 cgcggatatc ggcggaaagg gggtgcaggc gtccggaatc catgtatgca gcgagtatat
     1261 cgcgaaccgc ggccattttg ctataatcga gggaacgcgc ggcaatcatg agtctcgagg
     1321 tactcgccta ccccttcatc ttcctcggcc tctatttcga ggtcttcatg ctgctcacct
     1381 tcctgtcggc gccggctcga gagcgccggc tacgcgcggc ctcgcaggcg acgcccaccg
     1441 tggcgatcat cgtgccctgc tggaacgagg agagcaccat cggcggcacg gtccgctcgc
     1501 tcctcgccat ggactacccg aaggacaagc tccagatcgt gctcgtcaac gacggctcca
     1561 ccgacaacac ccgggtggtc atggattcct accgcgacca tccgcagatc accgtgctcc
     1621 ataaggagaa cggcggcaag cacaccgcga tcaacgcggg catcgaggtg gccaaggacg
     1681 ccgagatcgt cgggtgcctc gacgccgatt ccttcgtcgc tcccgacgcg ctccgcgaga
     1741 tgatcgcctg cttcgaccat cctcgcgtca tggccgcaac gcccgcgatg tcggtgcatg
     1801 agccgaagaa cttcctcgag ggtatgcaga acgcggagta catcatgggc atcgcgctgc
     1861 gccatgcgct ctcggccgtc aacggcatct acgtgacgcc cggtcccttc tccctctacc
     1921 gccgcagcat catcgaggag ctcggcgggt tccgccacgc acaccaggcc gaggatatgg
     1981 agatggcgct gcgcatccag aaggcaggct acgagatcga gaatgcaccg cgggcccggg
     2041 tgtacacgaa ggtgccccgc accgtgccga aactgatcaa gcagcgcacc cgttggacga
     2101 ccggcttcct ccggaacgtc accatggact accgcggcct cgtcgggaac ccgaagtacg
     2161 gcgtcctcgg gctcctcgtc ctccccttgg gcttcgtcgc catcatgggc ggcatcggcc
     2221 tcttcgtggt cgcggtggtg gagatcatcc gcgggctgct gcgcaccttc gcggtgaccg
     2281 ccggcatccc cctttcgtac acgctcgcgc cccgcttctc cctcgagtgg ttctatgcgc
     2341 cgatcacctt cgtgctcgtc atcagcgtga tcgtcaccgc gatcgccatc tccttcatgt
     2401 acatcgggcg ccacgtctcg aacaccccgg catcactgtc gctctccatc atcgggtacg
     2461 ccttcctcta cgggctcatt gcgcccttct ggctcctgcg gtccatcatc gatgtcgtca
     2521 ccggctaccg ccgtgcgtgg cgctaggagt gtagtagact cttgcctatg aagccctcgt
     2581 ccatgccgct gccgctccgc aacgcgctct tggcgctcat catcaccctc gtgatcatcg
     2641 gcaccatcgt cgcgaccatc aactacctcg accgccagcg gctcgcggag ctcgacgcct
     2701 tgcagaacca gctcgcgacc gacaccctct ccatcgagac acagttcgcg ctcctcgaag
     2761 aagcgccctg cgaggacctc gaggaaggca cggccttctc gcaggagctc gggaacttgg
     2821 gcgaccgcct ctcccggagc gagcagcggc tgggcagtga tgacccggaa gtggagcagc
     2881 tcaagaagca gtacaccctg ctgcagatcc gcgactatct gctgacgaag cggcttgcgg
     2941 agacctgcga cgtcgagccc gtgaccgcac tttacttcta ttcgaacgag gagagctgcg
     3001 aggactgcga tcgcgcgggc tatgcgctct cgtacctgcg cgagacctac ccaacgctgc
     3061 gcgtctactc cttcgactac gatctcgagc tcggggcgct ccagaccttg gtggccgtcg
     3121 agaaggtcga gccgcgcttc cccgccttcg tcatcaacgg caagcgctcc tacggcttta
     3181 ccgagcttga ggaattcgag acgcggttcc cgaaggcctt gtttgcgact acgaccgcga
     3241 cttccacgag aagtcgataa atggagccgc ctcccgggct cgaaccgggg acctacgcat
     3301 tacaagtgcg ttgctctacc aactgagcta aggcggcctg ccttactttt agcacactat
     3361 tccccgccct cctgcttctc ctgtatcgaa atgcggcgga gccgtaccgt gcggcgcacg
     3421 tgctgcaccg cctcgcggaa tgtccggcga ggacgctccg ggagctcctc cttcctgccc
     3481 cggagctggc gcacgaagcg ggtgagcacc cgctcgatga agcggacgag gagcagcgat
     3541 gtatgcgcga tgtcatgcag cacccgctcg atgccgtccc ggattgagtc gcggacgaac
     3601 gaccctaagt ctacgtgcga aagaatgaag gaggcctgtc cgaccttccg atccaatcgc
     3661 cggcgcacgc cgcccagaat gcgcacgcca tgggaggcct caaacgcggt cagggtcagg
     3721 aaccccacga ggaggatgag cgaaacgatg atggcgatga ggtagctcat gagagagccg
     3781 cgccgggatt atccccgcgc ggagaagcgc gcgaacttgg agacgaggac gcgctcaccg
     3841 aacttctggg aggcctcgtc gatgagctgc ttgacggtct tcgactcgtc cttgatgtac
     3901 ggctggttga ggaggaccga gtcccgaaag tagctgtcca tctttccggc gaggatcttc
     3961 tcctgcatct cggccggctt gtccttcacc tcctccttga agatcgcgag cgcagcggcc
     4021 ttctcctcct cgcggatctc ctcgctcgtg aggtactggg ggttggtcgc cgcgacctgc
     4081 atcgcgagct cgcgagcaag cgcctcgaac tcgggattct tcgcgacgaa gtccgtctcg
     4141 gaggcaagca ggaccatggc gccgatcgcc ttgtcgtgga gatagatgcc caccgccccg
     4201 gccccgatct cgcggtccga cttcttgtcc gcggcctcgg cggagcgctt tcgaaggatg
     4261 acctccgcct tctcgacatc gcctgcggcc tcctcgagcg ccttgcggca ctgcatgacg
     4321 gagagcccgg tagcctcgcg gagctgcttc acgacgtcga tagagatttc catgaatgaa
     4381 aagattatat acggattcaa acgctccccc gagggagcgt gggcttaacc gcgctggccc
     4441 tcctcgaagg cggttgcgag ctcgccgagc acgaggtcga tcgcggtacg cgatgcgtcg
     4501 ttgacgacga tcgggtaggc cgcgtccttg agatcgcagt ccgagctcat gatcccgatg
     4561 atcgggatgc cggcagcgcg cgcttccttg gttgcatgcg cctcgtgccg cgtgtccacg
     4621 atgacgagcg cgtcggggcg ctttgcaagc gaggtgatgc cggcgaggcg ctcggagagg
     4681 cgctcgatct cgcgatcgat gcggatgcgc tcgagcttgg tgtagatctt ggagagctcg
     4741 ccggtatcgc gcttcgcgga gaggtcggcg aggcggtcga tgcgcttctt aatctcggtg
     4801 aagttcgaga tggtcccgcc gagccagcgg ccggcgacgt agggctgcgc gaggcgcttg
     4861 ccggtcgcgg caaccgcgtc cgacgcctcc cgcttgccgc ccacgaagag cacggtcttg
     4921 ccgtcgcgcg cgagcgccgc gatggcggcc ttcgcggccg caagctgctc atcggtcttc
     4981 acgaggtcga agatctcgac cccgccctta ttgccgagga tgaaggagcg catcgaggga
     5041 tggcggcggc ttttgacgtg ggcaaaatgc gcgcccgcct cgaagaggcg gtccacgccg
     5101 gggcccgtgg tgctgctggt gctcatatga cctgcctagc ctaccatacc ggcttttccg
     5161 gctcaatccc ccgcctcggc cgggccgacc ccgcctgccg ctgccgcttc ggcgagcgcc
     5221 gtgacgatcg catcgatgcc gcctagcatg atcttcggga ggttatggaa attctgcttc
     5281 aggcgatggt ccgtgacgcg gtcctgcggg aagttgtagg tgcggatctt ctcggagcgg
     5341 tccccggtcc cgatctgccc ggagcgcagg tccgcatgct gctttgcctc ctcttctatg
     5401 gcgatggcct cgagtttcgc cgcgaggatg gccatggcct tctcgcggtt ggcgagctgg
     5461 ctgcgctcgc ttgacgagcg cacgtccacg ccggtcggct tgtggatgag ccggaccgcg
     5521 gtctcgacct tgttcacgtt ctggccgcct gccccgccgc tcctcgtgaa ctccatctcg
     5581 atgtccgcgg ggttgatctc cacgaccggc ttctggcgga ttgggagcgc ggccacggag
     5641 gccgtcgagg tatggatgcg ccccgatttc tcggtcaccg gcacgcgctg cacgcgatgc
     5701 acgccggtct cgtagcggag cgcgtcgtac gcgtcggtgc ccgcgatctc gagcgtgagg
     5761 tcgtcgatga cccgcacctt ccagccgcgg ctctcggcgt acttcatgta catgtcgcgg
     5821 agctcacccg cgaagagtgc ggcctcgtcc ccgcccgcgc ccgcgcggaa ctcaaggacg
     5881 agcgcggtgg gctttgcggc ctcctccttg tccttctcta tgatcttctc gatgtccttc
     5941 aggatgtcct gctgccgtgc gcgcaccctc gcctcgtcct cctccgccat cagagcaagc
     6001 tcggggtcgt tcccggctgc cgcgcgggat tcctccgcct ccttctggag acgctcgtat
     6061 tcctccgcga ggtacgcggt cttgtggttg gccttgaatt cgggggaata ttccatggta
     6121 ggtagatgat aacgcacgag cgccccgccc gaaggcgagg cgctcgcggc gtggtcgtta
     6181 cttcttcttg gctgcggcag cgcgctgctt gaagcgctcc acgcgccccg tcttgtcgag
     6241 cgtgcggtcc tcgttcgtat agaaggggtg ggacttcgag gagatctcga cggtgacctt
     6301 cgggtactcc ttgcccttat aggtgccggt ctccgtcgtc cggatggtcg agccgatgat
     6361 gaagacctcg cccgaggcga tatcctcgaa catgacctcg cggtaatcgg ccggatggat
     6421 gtcctttttc attgccgagg cactatacca gagccccgca ctcccttcaa gccgacccta
     6481 gaactcctcg tatctcggga tcacattcac cactgcgtcc ttccagaggc tgccgcactg
     6541 gagccggtac gtggtctggg cgctcacgct gccgcctgag accgggccgc cgctcgagct
     6601 gcacgaagcc gcattatgcg cgctcgagcc gatctgcgta tccggtccca ggatgcgcac
     6661 ggtgcaggcg ctatcgatgc cgcttgcggt ccaggagacg ctgatggccg taggggtgcc
     6721 ggccacgatg cgggcgggtg ttgcggtgat atcgaggggg ccgcttgcct gacaggaggc
     6781 gctcaccaca tcgacgctgc agtagttgga gaacacgccg ccgccttccg cgcggacgct
     6841 gccgtcgctg ttgaactggc gctggagggt cgctccgctg ccgatgatgt tgctgctgcc
     6901 gtcgtaccag gtgtaggggc cgcttgcgcc gttctgcggg gtcgcggtga aggtgatggt
     6961 cgtgcccggc gccacattat tggattgcgc gggagagcag gtgatggagg gggggttgcc
     7021 gctatcgcct ggatcgtcga agcagtcagg gtaggtgccg atctggccct ggtcctcaca
     7081 ggtcgggtct ggcggtggcg gatcggagca gtccgggtag gtgccgatct gacctgctgg
     7141 gcaggacggt gggggcggcg agacacaatt gggcggagtg ccgacttgtc cgagctcctc
     7201 acaggtgggc tcagggtccg cgacgcagtt gggataggcg cctgaatatc cggaggggca
     7261 gggacacccg tccatggtgg gttccgtttc gcaggtcggc cccgctatcg gacactcggg
     7321 atagggaccg gacgaaccgt ttgtacacga tgggggtggg ggcgggggtg ggcaccagct
     7381 gtccggggga ggctcagtcg ccgtacacac gcagctcccg ttgacatcct cgtacacgcc
     7441 gtaactgtac atattgcagg aattgggtcc tgcggagcag ctatcccctg ccatcgagca
     7501 ggacggtggc ggtggcggat tatccatgca cgactctgca taatcgcagg tgtagttccc
     7561 cacagtccat tcaccgcaga cttcgacgca tatatacgtt tggtttgggc cacagaacgt
     7621 atcgggatcg ctgagcggac agtagccgga ctcgtgctgc ccccgcacct cagtcggtgc
     7681 aatgacaccc atgtagagcg cgaagccaag tatcgcgatc gcgatcgcgt agatgagcat
     7741 tgtcggggta acagatcgaa ggatcgtcat agcgccatgg ttagtatcga aagggatgcc
     7801 ggcagggtca gcaccttggc cgatgacgac ccatcgagcg tacgcttcca gagctcctgg
     7861 ccctcatgcg tgcggcggag ctcgtatacg gccgcattac cgagcaccgg gctcgcgagg
     7921 tccttgaatg tgctgagtgg cgtgtatgcg ctcggggtaa tagaccccgc cacgctctcg
     7981 ccggtcattg catccgtcca aagcacagtc cgacgatccg gtgaataccg gacgcccgag
     8041 ctcagtaccg tcccggcagg ataccggaat aggagcgtcg cctctccggt ctgggtatcc
     8101 atcacggcaa tgccggcatc ggtgaagtag ctgatatgcg tgtcgtcgag gaatgcggga
     8161 gcgtagccgt aggcgatgga ctgcactccc gagccttcgg tcggacgcat gagcacttgg
     8221 tacgcacttg gcgtcacctt ctctgcctgc acgaacggca tgattgcctg cgtcgaggtc
     8281 gagatggcga atacgacccg gctgccgtcg ggcgagcggc tccctgagag ccagagggtg
     8341 tcccctgcag cgttacgcac gaactgcagc ccctcgatgt gttgcgggcc atacgtgggc
     8401 gccccggtat agaaatgagc gccccggagt
//
LOCUS       DASPKG010000032         5664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_462570, whole genome shotgun sequence.
ACCESSION   DASPKG010000032 DASPKG010000000
VERSION     DASPKG010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462570"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VEA36_01165"
     CDS             complement(<1..200)
                     /locus_tag="VEA36_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28957.1"
                     /translation="MDEQLSAPQPPGLHPHLVRAVIAGVVLVIIAALASAYFLYQPFR
                     TSIDEAMTVLRIPHELRGTHFYT"
     gene            complement(254..1600)
                     /locus_tag="VEA36_01170"
     CDS             complement(254..1600)
                     /locus_tag="VEA36_01170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HYF28958.1"
                     /translation="MRGRFLAIALVAIFIAGASVLPEAAGAQNPNLSPEQRAALERQL
                     ASLEKEIAQTQGTIDTLQTQGESLKRDIAILDGEIKKAKLRVRATEVAIQQLTGNIET
                     HSRNISMLTTKLADEKDSLAQIMRNTQQIDDYSLIEVVMSAQNVSEFFGDLDDFAAVN
                     AALYKSFDRIVSTRTETEGAKVELEGERLSQQQLRQMHLVEQQNVQKQEAEKRDLLAR
                     TKGEEALYRQLKNAQEKTAAQIRATLFPLRDTSGIPFGTAVEHAKAASAKTGVRAALI
                     LAILSQESDLGKNVGQCLITSLETGDGKGKNTGNPFPGTMKVPRDTVPFQRIMDALGR
                     DWSTTPVSCPLPGGYGGAMGPTQFIPSTWAAYEGRLAGALGVAATDPWNPSHAIMATG
                     LYLQDLGAAAGGGAEHRAAAQYYAGGNWATSGQGYANSVMGKAANFQRDIDILNAN"
     gene            complement(1675..2316)
                     /locus_tag="VEA36_01175"
     CDS             complement(1675..2316)
                     /locus_tag="VEA36_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HYF28959.1"
                     /translation="MITLQASAREATGKAARKEAAASSTMPAVVYGPKQEAMSISIGL
                     ADFERVLRNEGESTLIELSGLAKPVQVLIHEVDVDPVTSRPRHADFYAIEKGAKVKVS
                     IPLSYVGESPAVKAGANIVKVLHEVEVEADPSKLPHEIEVDITALAAEGDQVHVSDLK
                     APAGVTFVTDGDEVVALAQAVEVEPEEPAAALDMDAIEVEKKGKEEGEEGAAG"
     gene            complement(2356..3357)
                     /locus_tag="VEA36_01180"
     CDS             complement(2356..3357)
                     /locus_tag="VEA36_01180"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HYF28960.1"
                     /translation="MNDLLEIARALVHPGKGILAADESTKSADARLASYGIATGEEMR
                     RKERDMLLSCPGLEEYLSGVILYDETLRQNGEDGRPFGEILSSRGIIPGIKVDEGTEP
                     APESPDELITKGLLGLPERLTEYREQFGTGFTKWRAVVKIDGDRLPTRQVMVENAKRL
                     AEYSALVQKAGMVPMVEPEVLLEGNHSRMRSRQVLEETLSVLVEALENHAVDMSGVIL
                     KTAMAISGSTSGRTDTPEEVAEDTVGALMNVMPRGLAGVVFLSGGQTPDQATDNLRAI
                     EREAKAKNAPWPMTFSYSRALQDEALRAWAGKDENVEVAREAYMKRLKSVYQAAKGE"
     gene            3390..4382
                     /locus_tag="VEA36_01185"
     CDS             3390..4382
                     /locus_tag="VEA36_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HYF28961.1"
                     /translation="MAKLDFLAIGDIVSEPFIRLKDAYIATNEHDEENLCMRFGDKIP
                     YDSVTFVPAVGNASNAAVAAARLGLASALRGYVGNDRYGKECLEVLEHEGVDTAYMVT
                     QAGKQTNYHFVLWYDTDRTILVKHEEFDYAVPEMEDNPTWVYLSSLAANSLSYHDALA
                     DWLIARPEIKFAFQPGTFQMSLGVQKLRKLYERSDLFFCNREEAARILGHHETGDIKA
                     LLTDIRGLGPKIAVITDGTDGAYAFDGSSMYFIPMYPDTRGPYERTGAGDAFASTVCA
                     ALALGKPLEEALLWGPINSMSVVQDVGAQKGLLARAELEAYLKSAPESYRISTI"
     gene            complement(4379..4525)
                     /locus_tag="VEA36_01190"
     CDS             complement(4379..4525)
                     /locus_tag="VEA36_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28962.1"
                     /translation="MKYRQGRDRQVSIYLAALIITIFGSGMVLVIVRAISEVDFTSVA
                     AVAI"
     gene            complement(4522..5520)
                     /locus_tag="VEA36_01195"
     CDS             complement(4522..5520)
                     /locus_tag="VEA36_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HYF28963.1"
                     /translation="MINPEAQLVANVFAKDIERKPTRDGFGTGTVEAGKADPNVVVLC
                     ADLKESTRAEWFEKVFPDRYIEMGVAEQNLATVSAGLSAVGKVPFFASYAAFSPGRNY
                     EQIRTTIALNNRRAIVCGMHAGVSVGPDGATHQMLEDIGLMRSLPGMNVIVPSDAEEA
                     RKAVLAAAAADHPSYIRFGRSGVPVYTTPATPFAIGKALTLWRSEKPKVALLATGAQA
                     YDALVAARALDADGIGTIVLSVPSVKPLDEAAVLAAAKETGRVITIEEHQTAGGFGSA
                     VAEYLSSVHPVRVVRMGVVDAFGQSGEPAELLAHYKLDSVAIAARARELTGEQTTI"
BASE COUNT          930 a         1907 c         1764 g         1063 t
ORIGIN      
        1 gtatagaaat gagtgccccg gagttcatgg ggtatccgaa ggaccgtcat ggcctcatcg
       61 atcgaggttc gaaacggctg atagaggaaa tatgcagacg cgagcgccgc gatgatcacg
      121 agcacgacgc cggcgatgac cgcccgaaca aggtgggggt gcaagcccgg cggctgcgga
      181 gcggagagct gctcgtccat actcctcagt atacgtctat actgcattcc ttcgcgccgc
      241 gtctgtggac tacctaattg gcattcagga tatcgatgtc ccgctggaag ttcgctgcct
      301 tccccatcac gctgttggcg tacccctgtc cggaggtcgc ccagttgccg cccgcgtagt
      361 actgcgcggc cgcgcggtgc tcggccccgc cgcctgcagc cgcaccaagg tcctgcaggt
      421 agagcccggt cgccatgatc gcgtgcgaag ggttccaggg atcggtcgcc gcgaccccaa
      481 gcgcgcccgc gagccgtcct tcgtaggccg cccaggtgga cggtatgaat tgcgtcgggc
      541 ccatcgcgcc gccatatccg cccggaaggg ggcaggagac cggcgtcgtc gaccaatcgc
      601 ggccgagcgc atccatgatc cgctggaaag gcacggtatc gcgcggcacc ttcatcgtgc
      661 ccgggaacgg gttccctgtg ttcttgccct tgccgtcgcc ggtctcgagg ctcgtgatga
      721 ggcactgccc cacgttcttc ccgaggtccg attcctgcga gaggatggcg aggatgagcg
      781 cggcgcgcac cccggtcttc gccgacgcgg ccttcgcgtg ctcgaccgcg gtcccgaacg
      841 ggatgccgga ggtgtcgcgg agcgggaaca gcgtcgcgcg tatctgcgcg gcggtcttct
      901 cctgcgcgtt cttcaactgg cggtagagtg cctcctcgcc cttggtcctc gcgagcaggt
      961 cgcgcttctc tgcctcctgc ttctgcacgt tctgctgctc gacgaggtgc atctgccgaa
     1021 gctgctgctg cgacaggcgt tcgccctcaa gctccacctt cgcgccctcg gtctcggtcc
     1081 gggtcgatac gatacggtcg aaggacttgt agagcgcagc gttgaccgcc gcgaagtcat
     1141 cgaggtcccc gaagaattcc gagacgttct gtgcggacat gacgacctcg atcagggagt
     1201 agtcgtcgat ctgctgcgtg ttgcgcatga tctgcgcgag cgagtccttt tcgtccgcga
     1261 gcttcgtggt gagcatggag atgttccggc tgtgcgtctc gatgttgccc gtcagctgct
     1321 ggatggcgac ctcggtcgcg cgcacccgca gcttcgcctt cttgatctcg ccatcgagga
     1381 tggcgatgtc gcgcttcagg gactcgccct gcgtctgcag ggtatcgatg gtgccctggg
     1441 tctgggcgat ttccttctcc aaagaggcga gctggcgctc caaggccgct cgctgctcgg
     1501 gcgagaggtt ggggttctgg gcgcccgccg cttccggcag gacggacgcg cccgcaatga
     1561 agatcgcaac gagtgcgatg gcgaggaatc ggccacgcat ataggtatcg agtatatcat
     1621 tatgacgaaa gaggccgcgc gaactgtcgc gcggcctctt catgaaagcg gaggttatcc
     1681 ggctgctccc tcctcgccct cctccttgcc cttcttctcg acttcgatcg cgtccatatc
     1741 aagcgcagca gcgggctcct cgggctcgac ctcaacggcc tgcgcgagcg cgacgacctc
     1801 gtcaccgtcg gtcacgaagg tgacgcctgc gggagccttc aggtcgctca cgtggacttg
     1861 gtcgccttcg gcggcgagcg ccgtgatatc gacttcgatc tcgtgcggga gcttcgatgg
     1921 atccgcctcg acctccactt catggagcac cttcacgatg ttcgcgcctg ccttcaccgc
     1981 cggggactcg ccgacgtacg agagcgggat ggagaccttc actttggcgc ccttctcgat
     2041 agcatagaaa tcggcatggc ggggacggct cgtgaccgga tcgacatcca cctcatggat
     2101 gaggacctgg acgggcttgg cgaggcccga cagctcgata agcgtggact cgccctcatt
     2161 gcggagtacg cgctcgaaat cggcgagccc gatcgagatg gacattgcct cctgcttggg
     2221 gccgtagacg accgcgggca tggtcgacga cgcggctgct tctttgcgcg cagccttgcc
     2281 ggtcgcctca cgggcgctcg cttgcaacgt aatcatatgg aggtgagtat gccgtatcta
     2341 ctcggtttcc gcaacttatt cgcccttggc cgcctgatac acgctcttga ggcgcttcat
     2401 gtacgcctcg cgcgctacct cgacgttctc gtccttgccg gcccaggccc gcagcgcctc
     2461 gtcctgcagc gcccgcgaat atgagaaggt catgggccag ggcgcgttct tggccttcgc
     2521 ctcccgctcg atggcgcgca ggttatcggt cgcctggtcg ggggtctgcc cgccggagag
     2581 gaacacgacg cctgcgagcc cgcggggcat gacgttcatc agggcgccga ccgtgtcctc
     2641 cgccacctcc tccggcgtgt cggtcctgcc gcttgtactg cccgagatcg ccatcgcggt
     2701 cttgaggatg acccccgaca tatcaaccgc gtggttctcc aaggcctcga cgagtacgga
     2761 aagcgtctcc tcgagcacct ggcgactccg catccggctg tggttgccct cgagcaatac
     2821 ctcgggctcc accatgggca ccatgcccgc cttctggacc agagcggaat actcggcgag
     2881 gcgcttggcg ttctccacca tgacctgcct ggtcggaaga cggtccccgt cgatcttcac
     2941 gactgcacgc cacttcgtaa agcccgtccc gaactgctcc cggtattcgg tcaagcgttc
     3001 cggaagccca aggagcccct tggtgatgag ctcgtcgggg ctctcgggcg caggctcggt
     3061 cccctcgtcc accttgatgc ccgggatgat gccgcgcgac gacaggatct ccccgaacgg
     3121 ccggccgtcc tccccgttct gccgcagcgt ctcgtcgtag aggatgacgc ccgagaggta
     3181 ctcctcgagg cccgggcagg acaggagcat gtcgcgctcc ttgcgccgca tctcctcccc
     3241 cgtcgcgatg ccgtaggacg caaggcgcgc gtccgcgctc ttcgtgctct cgtccgcggc
     3301 gaggatgccc ttcccgggat gcaccaaggc gcgcgcaatc tcgagcaaat cgttcatacc
     3361 gtgcatggta gcatgctaga atttttcaaa tggcgaaact cgatttcctc gcgatcggcg
     3421 acatcgtgtc cgagcccttc atcaggctga aggacgcgta catcgcgacc aacgagcatg
     3481 acgaagagaa cctctgcatg cgcttcgggg acaagatccc ctacgactcg gtcaccttcg
     3541 tgcctgcggt cggcaacgca tcgaacgcgg cggtcgcggc cgcgcgcctc gggctcgctt
     3601 ccgcgctccg ggggtacgtc ggcaacgacc gatacggaaa ggaatgcctc gaggtcctcg
     3661 agcacgaggg cgtcgatacc gcgtacatgg tgacgcaggc cggcaagcag acgaactacc
     3721 atttcgtcct ctggtacgac acggaccgca ccatcctcgt gaagcacgag gagttcgact
     3781 acgccgtccc cgagatggag gacaacccga cctgggtgta cctctcgtca ctcgccgcca
     3841 attccctcag ctaccacgac gcgctcgcgg actggctcat tgcgcggccg gagatcaagt
     3901 tcgccttcca gcccggcacc ttccagatgt cgctcggggt gcagaagctc cggaagctct
     3961 acgagcggag cgatctcttc ttctgcaacc gcgaagaggc cgcgcgcatc ctcgggcatc
     4021 acgagacggg cgacatcaag gcgctcctca ccgatatccg tgggctcggc ccgaagatcg
     4081 cggtcatcac cgatggcacg gacggcgcct atgccttcga cggctcctcg atgtacttca
     4141 ttcccatgta tcccgatacg cggggacctt acgagcgaac gggcgcgggc gatgccttcg
     4201 cctcaaccgt atgcgcagcg cttgcgctcg gcaagccgct cgaagaggcg ctcttgtggg
     4261 ggcccattaa ctccatgtcg gtggtgcagg atgtcggcgc gcagaagggg ctcctcgccc
     4321 gcgcggagct tgaggcgtat ctcaaaagcg cccccgagtc ctatcggatc tcaacaattt
     4381 aaatcgcgac cgcggcgacc gacgtgaagt ccacttcgct tatcgcgcgc acgatcacga
     4441 gcaccatgcc gctcccgaag atcgtgatga tgagcgcggc aagatagatg ctcacctgtc
     4501 ggtccctgcc ttgtcggtat ttcatatcgt tgtttgttct ccggtaagtt cccgcgcccg
     4561 ggcggcgatc gctacgctat cgagcttata gtgcgccaag agctccgcgg gttcccccga
     4621 ctgcccgaag gcgtccacga cgcccatgcg cacgacgcgc accggatgca cggacgagag
     4681 gtactccgcg accgcgctcc cgaagccgcc cgccgtttgg tgctcctcga tggtgatgac
     4741 ccggccggtc tccttcgcgg ccgcgagcac cgccgcttca tcgagcggct tgaccgaggg
     4801 caccgagagc acgatcgtgc cgataccatc cgcatccaag gcgcgcgcgg ccactaatgc
     4861 atcgtacgcc tgcgcgccgg tcgcaaggag cgcgaccttc ggcttctcgc tccgccacag
     4921 ggtcagcgcc ttcccgatcg caaagggcgt cgcgggcgtc gtatagaccg ggacgccgct
     4981 cctgccgaac cgtatatagg aagggtgatc ggcggccgcg gccgcgagca cggctttacg
     5041 cgcctcctcg gcgtccgatg gcacgatgac gttcatgccc ggcagcgatc gcatgagccc
     5101 gatgtcctcg agcatctggt gggtcgcgcc gtctggcccc accgataccc ccgcatgcat
     5161 gccgcagacg atggcccgcc ggttattgag ggcgatcgtc gtcctgatct gctcgtagtt
     5221 cctgccaggc gagaaggccg cgtaggaggc gaagaagggc accttcccga cggccgagag
     5281 ccccgcggag acggtggcga ggttctgctc ggccaccccc atctcgatat accggtccgg
     5341 gaacaccttc tcgaaccatt ccgcgcgcgt ggattccttg aggtccgcgc acaagaccac
     5401 tacattcggg tccgccttcc ccgcctccac cgtgccggta ccgaagccgt cgcgggtcgg
     5461 cttcctctcg atatccttcg cgaacacgtt cgcgacgagc tgtgcctcgg gattgatcac
     5521 gttggaagta gtatagcgcg ctagccgagc cggccgagga gcccggcgat caataatata
     5581 agtatgacga tcccggggac gagcgggatg agcaggagga ccttcagggc gcgcctgcgc
     5641 cccgccgcgc tcacctcccg gagc
//
LOCUS       DASPKG010000033         6611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_57041, whole genome shotgun sequence.
ACCESSION   DASPKG010000033 DASPKG010000000
VERSION     DASPKG010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57041"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VEA36_01200"
     CDS             complement(<1..697)
                     /locus_tag="VEA36_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HYF28964.1"
                     /translation="MTYRPTIGLEIHAELNTKTKMFCASANDPDETRPNVNVCPVCMA
                     HPGTLPVPNQEAVRHIARVGTAVGGTLADYTEFDRKSYFYPDIPKGYQISQYEHPLVK
                     GGELVGVPITRVHLEEDTARSTHSDGGSLVDFNRAGVPLMELVTEPEIHDAETAGNFA
                     RELQLLLRTLGASHANLEKGEMRIEANISVSKDDTLGTKVEVKNLNSFRSVERAIEFE
                     IARQSELLERGERV"
     gene            complement(742..1716)
                     /gene="obgE"
                     /locus_tag="VEA36_01205"
     CDS             complement(742..1716)
                     /gene="obgE"
                     /locus_tag="VEA36_01205"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HYF28965.1"
                     /translation="MAFVDEMHVRAQAGRGGNGVVRWLHLKGLEKAGPAGGDGGRGGD
                     IVLEGVRDLSILSSYRFTKSFRAEDGGAGEGNDKHGKNGEPLVIKVPIGSVAVTSTGE
                     TYEILHEGEQITVFKGGAGGLGNAHFKSSVNQNPFESTKGKPGQGGDISITLKLIADA
                     GFVGFPNAGKSSLLNSLTRARSRVAAYPFTTLDPHLGDFYGYLLADIPGLIEGASAGR
                     GLGSKFLRHIERTRILVHLVSAEQEDPEKSYRAIRDELESFGRGLSDKPELVVLSKID
                     VLPPDEQGKAISTLSAAIGKEVLPLSVIDDDLLKAFSDRLAKELSAGR"
     gene            complement(1799..2008)
                     /locus_tag="VEA36_01210"
     CDS             complement(1799..2008)
                     /locus_tag="VEA36_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28966.1"
                     /translation="MYLRTRLVQVGEPAWTGEFHEGATLGDVVEAAGATVGDRALSQN
                     GRRASASDPVQPDSMVMLGPNARHG"
     gene            complement(2029..2655)
                     /locus_tag="VEA36_01215"
     CDS             complement(2029..2655)
                     /locus_tag="VEA36_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HYF28967.1"
                     /translation="MEEPVVPAITLIGVGGIGTALLPLLARLHPAQVTLWDNDIVNEH
                     NLVNQFLWRTGDVGKHKALVAGSRLAEATVAARAVPRRYEGIGRFDTIVIAAVDSMES
                     RRLVWNGAKGAGSGVQLFFDGRLSRETPFYFQLFAIRMDDSDAREAYEGWLEGDGEPD
                     TGRRNFDMVPAPLVLSGVIGTLIVRWSRKMDLPWQVIWDGASMSLVSH"
     gene            complement(2667..3221)
                     /locus_tag="VEA36_01220"
     CDS             complement(2667..3221)
                     /locus_tag="VEA36_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28968.1"
                     /translation="MSGRKKSRTRLTWRRTLRRNKQRRFELERQQRVRREAMIERRIG
                     IVRKRARQERNCTILGRDARVVRAFYVNDVLMFETTMMHVQIEDKKYRIGKFEVQLDP
                     TLAKPLTLCLESGRRDGREPKYYMTWGGDPWFCVGDRMDLLRYLVRSGEDLAAAYLML
                     EAFEHVNPEHHEEVAGTSIPVPIR"
     gene            complement(3293..5812)
                     /gene="secA"
                     /locus_tag="VEA36_01225"
     CDS             complement(3293..5812)
                     /gene="secA"
                     /locus_tag="VEA36_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HYF28969.1"
                     /translation="MASLLGKLFSQNESAGFVKAAQPVVAHAGALADELAALSQEELQ
                     ARLATVKERTGGLPTADADVAEVFALVREAADRTLHERHYDVQLIGGLALAKGRIAEM
                     RTGEGKTLVATLAATLRALAGKGVHVVTVNDYLARRDGAWMGQVYAYLGLSVGIVTSQ
                     GSYIYDASHTETEEDRARDEEGSFRVFYDFLRPVSKKEAYAADITYATNNELGFDYLR
                     DNTAYDPSQLTQRGHHYAIIDEVDSILIDEARTPLIISGPAANVEGLYERFSEIVRDF
                     AEGEDYTVDEKQRAVQITEAGIAKAEKALNLENLYTEGGMKYAHHLDTAVRAKALFHK
                     DKEYVVREQEIVIVDEFTGRLQPGRRWSEGLHQAVEAKEGVPIQRETRTYASVTFQNY
                     FRMYEALAGMTGTGLSSEEEFYTVYKLEVVAVPTHRPIARKDYDDLIFQTVQGKYIAL
                     TRNVKAIHEKGQPLLIGTVSIEDNEVISEHLKKAGVPHEVLNAKNHEREGEIIADAGK
                     KGQVTVATNLAGRGVDIKLGGALASEEERQAVRELGGLAVIGTERHEARRIDNQLRGR
                     SGRQGDPGETRFYVSLEDKLMRVFASDMIKNVMGSFKIPEDEPIESRMITRSLETAQK
                     RIEGFNFDSRKQVLAYDDVLNTQRLAVYTERRAALLGTNEEVDAHIRAKLATPEAIAT
                     YEAKRAEIGDEAFLPLMRHLILQVTDAFWLEHLETMDYLRRSVSLRAYGQRDPLIEYR
                     REGLHRFNAMQDSIAQALTEALPRIQVADESRIKAEEERTRRALVAASEGKEDAGPSE
                     PIRNTTTYGRNDEVTIKRGEETRTLKYKKAEPLLTEGWTIV"
     gene            5872..6510
                     /locus_tag="VEA36_01230"
     CDS             5872..6510
                     /locus_tag="VEA36_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28970.1"
                     /translation="MAAAPYGLTDEEYRLLASLDTPERIQDYLDTLAINFEKQGETCR
                     SPRRVLQTKKAHCMEGALLAYAALLIHKQKPLLLDLKSARGDDDHVIVLCRKDGRYGA
                     ISKTNHATLRFRDPVYQSVRELVMSYFHEYFLNKNGKKTLMSYSAPFSLAGFGTDWIT
                     AERDLWEIPKALDKSPHFPIASKETLARTRPADPIERAAGRLTEWKRTDRGT"
BASE COUNT         1095 a         2137 c         2092 g         1287 t
ORIGIN      
        1 agactctctc tccgcgctcg aggagttcgg actgccgcgc gatctcgaac tcgatcgcgc
       61 gctccaccga gcggaacgag ttcaggttct ttacttcgac cttcgtaccg agcgtgtcgt
      121 ccttcgacac cgagatgttc gcctcgatgc gcatctcccc cttctcgagg tttgcatgtg
      181 aggcaccgag cgtgcgcagg agaagctgca gctcgcgcgc gaagttccct gcggtctcgg
      241 catcatggat ctccggttcg gtgacgagct ccatcaaggg gacgcccgca cgattgaagt
      301 cgacgaggct gccgccatcg ctatgcgtcg agcgggccgt gtcctcttcg aggtgcacgc
      361 gcgtgatcgg gacgccgacg agctcaccgc ctttgaccag cggatgctcg tactggctga
      421 tctggtaccc cttcgggatg tccggataga aatagctctt gcgatcgaac tcggtatagt
      481 cggcgagggt gccgccgacg gcggtaccca cccgcgcgat gtgccggacc gcttcctggt
      541 tcggcaccgg gagcgtgccg gggtgcgcca tgcagaccgg gcagacgttc acgttcggcc
      601 gggtctcgtc cgggtcgttc gcgcttgcgc agaacatctt ggtcttcgtg ttgagctccg
      661 catggatctc gaggccgatg gtcggacgat acgtcatgat tggctacacg gtaccccgcg
      721 cgagcctggg tgcgcaagcg gctaccggcc cgccgagagc tctttcgcga gccgatccga
      781 gaaggccttg aggaggtcgt cgtcgatgac cgagagcggg agcacctcct tccctatcgc
      841 cgcggagagg gtcgaaatcg cctttccctg ttcgtcgggg ggaagcacgt cgatcttgga
      901 aaggaccacg agctctggct tatccgagag cccccgccca aaggattcga gctcgtcgcg
      961 tatggcccta tagctttttt cggggtcttc ttgttcagcc gagacgagat gtacaaggat
     1021 tctcgtgcgc tcgatatggc gcaggaactt ggagccgagg ccgcgtcctg ccgaggcgcc
     1081 ctcgatgagg cccgggatat cggcgagcag gtacccgtag aagtcgccga gatgggggtc
     1141 gagcgtggtg aaggggtagg ccgccacgcg ggagcgggca cgggtaagcg agttcaaaag
     1201 ggaggacttc cccgcgttcg ggaagcccac gaacccggcg tccgcgatca gtttgagggt
     1261 gatggagata tccccaccct gccccggctt gcccttcgtg gactcgaagg gattctggtt
     1321 caccgagctc ttgaagtgcg cgttgccgag gccgccggcg cctcccttga agacggtgat
     1381 ctgctcacct tcgtggagaa tctcgtacgt ctcgccggtc gaggtgacgg cgaccgagcc
     1441 gatgggcacc ttgatcacga gcggctcgcc gttcttcccg tgcttgtcgt tgccttcgcc
     1501 tgcgccgccg tcctccgctc ggaagctctt cgtgaatcgg tagctggaaa gtatagaaag
     1561 gtcgcgcacc ccttcgagta cgatatcgcc gccacggccg ccatcgccgc cagcgggtcc
     1621 cgccttctcg aggcccttca agtgcagcca acggacgacg ccattgccgc ccctgccggc
     1681 ctgcgcgcgc acgtgcatct catcgacgaa tgccatatgt cgcttaccct atcacaaaat
     1741 agaaagagcc gcggtgtcgc caccgcggct ctcatcttcc gtccgctgcc aggatggatc
     1801 agccgtgccg cgcgttcggc ccgagcatca ccatcgagtc cggctgcacg ggatcggacg
     1861 ccgacgcgcg ccggccgttc tgcgacaacg cccggtcacc gaccgtcgcg cccgcagctt
     1921 cgaccacatc cccgagggtt gcgccctcat ggaactctcc agtccaggcg ggttcgccga
     1981 cctggaccaa tcgcgtcctg aggtacatcg tcacactcct tccgctgctt agtgcgatac
     2041 gagcgacatg ctcgcaccgt cccagatgac ctgccagggc aggtccattt ttcggctcca
     2101 tcggacgata agcgtgccga tgacgccgct gagcacgaga ggcgctggga ccatatcgaa
     2161 gttcctacgc ccggtatcgg gttcgccgtc gccctcgagc catccttcgt aggcctcgcg
     2221 ggcatcggag tcgtccatcc gtatggcaaa gagctggaag tagaacgggg tctctcgcga
     2281 gagccgtccg tcgaagaaca gctggacgcc gctccccgct cccttcgcgc cgttccagac
     2341 gagacgacgt gactccattg agtctactgc cgcgatgacg atggtgtcaa agcggcctat
     2401 cccctcatac cgcctgggca ccgcccgagc agcaaccgtt gcctccgcca gccgtgagcc
     2461 agcgacaagc gccttgtgct tccccacatc gccagtccgc cagaggaatt ggttgacgag
     2521 gttgtgctca ttgacgatat cgttgtccca gagcgttacc tgcgcgggat gcagccgcgc
     2581 gagcagcggg agaagcgccg ttccgatgcc cccaacccca ataagggtga tggcgggtac
     2641 taccggttct tccatgattt cctccgctac ctgatcggca cgggaatgga cgttcccgcg
     2701 acctcctcat ggtgttcagg attcacgtgc tcgaaggcct cgagcatgag atatgcggcc
     2761 gcaagatctt ccccggacct cacgaggtat cggagcagat ccatgcgatc gccaacgcag
     2821 aaccaaggat ccccacccca ggtcatgtag tacttcggct ccctgccgtc gcgtctgccg
     2881 ctctcgagac agagggtgag cggcttagcg agggttggat cgagctgaac ctcgaatttg
     2941 ccgatgcggt acttcttatc ctcgatctgc acatgcatca tcgtcgtttc gaacatgagc
     3001 acgtcgttca catagaaagc gcgaacgacc cgagcatcac gtccgaggat cgtgcagttc
     3061 cgctcctgcc gagcacgctt acgcacgatc ccgattctcc gttcgatcat ggcctcgcgt
     3121 cggacccgct gctgccgctc gagctcgaac cgacgctgct tattgcgccg aagcgtacgt
     3181 cgccaggtga ggcgggtacg gctctttttt cttccgctca ctgggttact ccttccgatt
     3241 caaacaccag tctccccgga aggtactcca cttcctcgat tcgggcaatg ggttagacga
     3301 tcgtccaacc ctcggttaag agcggctcgg ccttcttgta cttcagggtc cgtgtctcct
     3361 cccctctctt gatcgtcacc tcgtcattgc gaccgtaggt agtggtgttg cggatgggct
     3421 ccgaaggacc tgcatcctcc ttcccttcgg acgcggcgac cagggcgcgg cgggtgcgct
     3481 cctcctccgc cttgatgcgg gactcgtccg cgacctggat gcgcggtaag gcctcggtga
     3541 gcgcctgcgc gatagagtcc tgcatggcat tgaagcgatg gagcccctcg cggcggtatt
     3601 cgatgagcgg atcgcgctgg ccgtacgcgc gcagcgagac cgagcggcgc aggtaatcca
     3661 tggtctcgag gtgctcgagc cagaaggcgt cggtaacctg gaggatgagg tggcgcatga
     3721 ggggaaggaa cgcctcatcc ccgatctccg cgcgcttggc ttcgtaggtg gcgatggcct
     3781 caggagtggc gagcttggcg cggatgtgcg cgtcgacttc ctcgttggtg ccaaggagcg
     3841 cggcgcggcg ctcggtatac accgcaaggc gctgggtatt gaggacgtcg tcgtaggcga
     3901 ggacctgctt gcgggagtcg aagttgaagc cctcgatgcg cttctgcgcg gtctccaagg
     3961 accgcgtgat catacggctc tcgatgggct cgtcctcggg gatcttgaag gagcccatga
     4021 cattcttgat catgtcggag gcgaagacgc gcatgagctt gtcctcgagc gacacgtaga
     4081 agcgggtctc gccagggtcc ccctggcggc ccgagcgccc gcgcagctgg ttgtcgatcc
     4141 ttcgtgcctc gtgccgctcc gtcccgatga ccgcgagccc gccgagctcg cggacggcct
     4201 gccgctcctc ctcggaggcg agcgcgccgc cgagcttgat gtcgacgccg cgacctgcga
     4261 ggttggttgc gaccgtgacc tgtcccttct tgcccgcgtc cgcgatgatc tcgccttcgc
     4321 gctcgtggtt cttggcgttc aagacctcgt gcgggacgcc cgccttcttg aggtgctcgc
     4381 tgatgacctc gttgtcctcg atcgagaccg tgccgataag gagcggctgc cccttctcgt
     4441 gaatcgcctt cacgttcctt gtgagcgcga tgtacttccc ctgcaccgtc tggaagatga
     4501 gatcgtcgta gtccttgcgg gcgatggggc gatgggtcgg gactgcgacc acctcgagct
     4561 tgtagaccgt atagaactcc tcctcggagg aaagcccggt gccagtcatg ccggccaagg
     4621 cctcgtacat gcggaaatag ttctggaagg tgaccgatgc gtaggtgcgg gtctcgcgct
     4681 ggatgggcac gccctccttc gcctcgaccg cctgatggag cccctcggac cagcggcggc
     4741 cgggctggag cctgcccgtg aactcgtcca cgatgacaat ctcctgctcg cggaccacgt
     4801 actccttgtc cttatggaag agggccttcg cgcgcaccgc ggtatccagg tgatgcgcgt
     4861 acttcatgcc gccctcggtg tagaggttct cgaggttgag cgccttctcg gccttcgcga
     4921 tgcccgcctc ggtgatctgc accgcgcgct gcttctcgtc gacggtgtag tcctcccctt
     4981 ccgcaaagtc tcgcacgatc tccgagaacc gctcgtagag gccctcgacg ttcgcggccg
     5041 ggcccgagat gatgagcggg gtgcgggcct cgtcaatgag gatcgagtcc acctcgtcga
     5101 tgatggcgta gtggtggccg cgctgggtga gctgcgaggg gtcgtaggcc gtattgtccc
     5161 ggaggtaatc gaacccgagc tcgttgttgg tcgcgtaggt aatatccgcc gcgtaggcct
     5221 ccttcttcga gacgggacgg aggaagtcgt agaagacgcg gaaggagccc tcctcgtcgc
     5281 gcgcccggtc ctcctcggtc tccgtatgcg acgcatcata tatataggag ccctgggagg
     5341 tgacgatgcc caccgagagc ccgaggtacg cgtagacctg ccccatccag gcgccgtcgc
     5401 ggcgtgcgag gtagtcgttc accgtcacga cgtgcacgcc tttgccggca agcgcccgca
     5461 gggtcgccgc caaggtcgcg accagggtct tgccctcgcc ggtccgcatc tcggcgatgc
     5521 ggcccttggc gagcgcgagc ccgccgatga gctggacgtc gtagtggcgc tcgtggagcg
     5581 tgcgatcggc cgcctcgcgg accagggcga acacctccgc gacgtccgcg tcggcggtgg
     5641 gcaggcctcc cgtccgctcc ttcacggtgg caagccgcgc ctggagctcc tcctgggaga
     5701 gggccgcaag ctcgtccgca agcgccccgg cgtgggcaac gacgggctgc gcggccttca
     5761 cgaagcccgc ggattcgttc tgcgagaaga gcttaccgag taatgaagcc atgcgggaca
     5821 ctataccctg cagcctgccg gcctgctagc gccttcctat actatggagg catggcagca
     5881 gcgccctacg gattgaccga cgaggagtac cggctgctcg cttccctcga tactccggag
     5941 cgcatccagg actatctgga taccctcgcg atcaatttcg agaagcaggg ggagacctgc
     6001 aggtcacccc ggcgcgtgct gcagacgaag aaggcgcact gcatggaggg cgccttgctt
     6061 gcgtacgcgg cgctcctcat tcataagcag aagccgctgc tgcttgacct caagagcgcg
     6121 cggggcgacg acgatcacgt gatcgtgctg tgtcggaagg acggccggta cggcgcgatc
     6181 agcaagacga accatgcgac gctgcgcttc cgtgatccgg tgtatcagag cgtgcgcgag
     6241 ctcgtcatgt cctacttcca cgagtacttc ctcaacaaga acgggaagaa gaccttgatg
     6301 agctactccg cccctttctc ccttgcaggc ttcggtaccg actggatcac cgccgagcgc
     6361 gacctctggg agatcccgaa ggcgctcgat aagagcccgc acttccccat cgcgtccaag
     6421 gaaacgctcg cgaggacgcg gcccgcggac cccatcgagc gggcggccgg acggctgacc
     6481 gagtggaagc ggactgatcg gggcacctga atgcgaaaaa cgcccgagtg ggcgtttgtc
     6541 gcgagaacgc gatgaagcta tttgcgcttc ttcgtgctct tcttcgctgc cttctttgca
     6601 gggcggcgct t
//
LOCUS       DASPKG010000034         4744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1411178, whole genome shotgun sequence.
ACCESSION   DASPKG010000034 DASPKG010000000
VERSION     DASPKG010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411178"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VEA36_01235"
     CDS             <1..1174
                     /locus_tag="VEA36_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HYF28971.1"
                     /translation="LSSIFPFVSFDLTSDRGILYGINRHNASLILFDRFSLENYNSVV
                     FAKSGSGKSYAVKLEILRSLMFDTDVIVIDPEREYEYLAEATGGRNFNISLSSEHHIN
                     PFDLPAVKEDEEPSDILRSNIINLVGLFRIMLGGLSPEEDAIIDRAISETYALKDITG
                     DADFTGMEPPLMSDFENVLSGMEGSESLVTRLSKYTHGTWAGFLNRPTNVDINKQFVV
                     FSVRDMEDELKPVAMYIITHFIWNAVRKELKKRLLVIDEAWWMMKSEDTASFLYSLAK
                     RGRKYYLGLATVTQDVEDFLRSPYGRPILTNSSLQLLLKQSPTTIDVLQETFNLTDEE
                     KYLLLESGVGEGLFFAGLKHVAIKVVASYTEDQIITSDPSQLLAIKKEKERRETEK"
     gene            1177..1701
                     /locus_tag="VEA36_01240"
     CDS             1177..1701
                     /locus_tag="VEA36_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28972.1"
                     /translation="MPAPLIGVAIGTAGRAAAGGAARVAGRRALGRGVAAARRLPRRR
                     YNDDRQGEGTEPGRRNIKRIDAVNIFFLVIVAVVIDVLQFFLTLTLVGSLVAFILSIA
                     AWVLFFLWFLSLGVNYFNKGNAMKFLVGLGSVCAELVPFLNAITPALTAGVVAVIILH
                     NLEAAEEERLRAAA"
     gene            1748..2722
                     /locus_tag="VEA36_01245"
     CDS             1748..2722
                     /locus_tag="VEA36_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28973.1"
                     /translation="MGIRVLLVAVGAFLLPLTMHAQFAGVEPLEISITPEYPRPYQTV
                     SVRPSSTLIDLAAASVTVSANGTVVGQGSGSAPVNVALGGPGSLTTIRVSATVEGKTY
                     TRELRVRPAEVSLIVEPQTSRHPFYAGGTGVASEGQLRLVAISDLRSSAGTRLDPATL
                     VYTWRLGDRILEAQSGIGKYYMSASAPVRYRDTVVSVTVTTQDSAIVSRAEVLVAPID
                     PLVRIYRNDPLLGPLFDTALSGTVTMDDEETFRAVPYHFAGGSGITWLVNGAENGSDR
                     DITLRATGTGGGSASLIARVKQAATFALAEARLGVKFGEERALGIFGL"
     gene            2719..3534
                     /locus_tag="VEA36_01250"
     CDS             2719..3534
                     /locus_tag="VEA36_01250"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HYF28974.1"
                     /translation="MKKLLVLIALVVLLVPIGALAQSDANCEGGFCALTNIKNVEFAS
                     NAAELPDFLNALYMICIGLAAVIGVLQIMRAGVTWMTAAGSHEKIGDAKKLVRDTIIG
                     LVLVLAPTIVFGIINPKILSLEIGCLEGLKKGAVDCAGNVKTPEATPGSSGPVCGDYR
                     KKQVVPGNMVCSAKNGYSKINNDCCGDVPEGSQCCGNPYGPDNPPPSMIPNTIGTDKP
                     EPNPACRVTYKDGNLLPDGDQAQQFCCDYQDAYGIQCTALKNPRGAGLYCSCQ"
     gene            3537..>4744
                     /locus_tag="VEA36_01255"
     CDS             3537..>4744
                     /locus_tag="VEA36_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28975.1"
                     /translation="MRRILIIIAAVILLIILIVGAYFLFFRGGGGLIVGPDPFGPAGD
                     RDPGLAPVVDTGVEVPGAGTSIGPRLLQLTDRAVAKGTVSLYTPPRTVTVASSTSTST
                     PPVVETTPAEVEVRYIDRQSGNVYSFKVHERVLTRLSNKTLPGVIEASWTRDGSRAFV
                     RFLERDVAGAERVSTYLLPANGEGGFFLEQGLAQVEVASTTVLTLLSTDTGSVASAMN
                     ADGTGTRTLFSSVLSSLRVAYLGTSYLAQTKPTAHLDGYAFIVSGGTFTRLLGPLRGL
                     STLPSPSGRNVLYSYVDRGKLYTQVLDVSARTTLPLPLATLPEKCVWTPDGSALYCGV
                     PTSVAGLLPDDWYQGARPFSDRVWRIDMATRVATLVFDPRQIAQVSIDAQALSLDSAS
                     DVLVFMNKSDG"
BASE COUNT          824 a         1538 c         1495 g          887 t
ORIGIN      
        1 cctgtcctcg attttcccgt tcgtctcctt cgatctcacc tccgaccgag gcatcctgta
       61 cggcatcaat cggcataacg cgtccctgat cctcttcgat cgcttttcgc tcgagaacta
      121 caactcggtc gtgttcgcga agtcgggctc cggcaagtcc tacgcggtga agctcgagat
      181 cctgcgctcc ctgatgttcg atacggacgt catcgtcatc gacccggagc gggagtacga
      241 gtacctcgcg gaggcgaccg ggggacgcaa tttcaacatc tcgctctcct ccgagcacca
      301 catcaatccg ttcgatctcc ccgcggtgaa ggaggacgag gagccttctg acatcctccg
      361 gagcaacatc atcaatctcg tcggcctctt ccgcatcatg ctcggcgggc tcagccccga
      421 ggaggacgcg atcatcgacc gagccatttc cgagacctac gccctgaagg acatcaccgg
      481 agatgcggac ttcaccggca tggagcctcc gctgatgagc gatttcgaga acgtcctttc
      541 gggcatggag ggaagcgaat ccttggtcac gcggctctcg aagtatacgc acggcacctg
      601 ggcgggcttc ctgaatcgcc ctacgaacgt ggatatcaat aagcagttcg tggtgttctc
      661 ggtccgcgat atggaggatg agttgaagcc ggtcgcgatg tacatcatca cccacttcat
      721 ctggaacgcg gtccgcaagg agttgaagaa gcgcctgctc gtcatcgacg aggcatggtg
      781 gatgatgaag tcggaggata ccgcctcctt cctctacagc ctcgccaagc gcgggcgcaa
      841 gtactatctc gggctcgcaa ccgtgacgca ggacgtcgag gacttcctcc ggtcccccta
      901 cggccgtccc atcctcacga actcctcgct ccagctcctt ctgaagcagt ccccgacgac
      961 catcgacgtg ctccaggaga cctttaacct caccgacgag gagaaatacc tcctgcttga
     1021 gtccggggtg ggggaggggc tcttcttcgc gggcctgaag cacgtcgcga tcaaggtggt
     1081 cgcctcctat accgaggacc agatcatcac gtcggacccg tcgcagctgc ttgcgatcaa
     1141 gaaggagaag gagcggcgtg agactgagaa gtaattatgc ctgcgccgct catcggagtc
     1201 gctataggga cggccgggcg cgcggccgca ggaggtgccg cgcgcgttgc gggacgaagg
     1261 gcgctcggta gaggggttgc tgccgcccgc cgattgccga gacggagata taacgacgac
     1321 cgtcaaggag agggtacaga gccaggccgg aggaatataa agcgcatcga cgcggtcaat
     1381 atcttcttcc ttgtcatcgt cgcggtcgtc atcgacgtgc tgcagttttt cctcacgctc
     1441 acgctcgttg gcagcctcgt ggccttcatc ctctcgatcg ccgcgtgggt gctctttttc
     1501 ctctggttcc tgtctctcgg cgttaattat ttcaataagg ggaacgcgat gaaattcctc
     1561 gtcggcctcg gatcggtatg cgcggagctc gtacccttct tgaatgcgat aacaccggcg
     1621 ctcactgccg gtgtggtcgc cgtcatcatt ctccataatc ttgaggcggc cgaagaggag
     1681 cgactgcggg ccgctgcata agtgggatac cggccaatgc cggagggcgg cggtatatac
     1741 taaactcatg ggaattcggg tccttctcgt cgcggtaggt gcattcctgc ttccgctcac
     1801 catgcatgcg cagttcgcgg gggtcgaacc gcttgaaatc tccatcacgc ccgaatatcc
     1861 gcgtccgtac cagacggtct cggtgcggcc gtcgagcacg ctcatcgacc ttgccgcggc
     1921 ctccgttacc gtgagcgcca atggcacggt cgtggggcag ggcagcggga gcgctccggt
     1981 caacgtcgcg cttggcggcc ccggcagcct caccactatc cgcgtgagcg ccaccgtcga
     2041 ggggaagacg tataccaggg agctgcgcgt gcgtcccgcg gaagtctcgc tcatcgtcga
     2101 gccgcagacc tcgcgtcatc cgttctatgc gggagggacg ggcgtggcgt cagaagggca
     2161 gctccggctc gtcgccatct cggacctccg cagctcggcg gggacgcgcc tcgatccggc
     2221 tacgctcgtc tatacctggc gactcgggga ccggatcctc gaggcgcagt cgggcatcgg
     2281 gaagtactat atgtccgcga gcgcaccggt ccgctaccga gatacggtcg tgtcggtcac
     2341 cgtcacgacg caggatagcg cgatcgtgtc gcgcgccgag gtgctcgtcg ctccgatcga
     2401 tcccctggtc cgcatctatc ggaacgatcc cttgcttggc cccctcttcg ataccgcgct
     2461 ttcgggtacc gtgaccatgg acgacgagga gaccttccgg gcggtgccct accacttcgc
     2521 gggcggatcc gggatcacct ggctcgtcaa cggcgccgag aacgggagcg atcgggatat
     2581 cacgctgcgc gcgaccggta cgggcggcgg gtccgcgagc ctcatcgcgc gggtgaagca
     2641 ggcagccacc ttcgcgctcg cggaggcgcg gcttggggtc aagttcggag aggagcgggc
     2701 gctcggcata ttcggcctat gaaaaagctt ttagtactca tcgcgctcgt cgtgctcctc
     2761 gttccgatcg gggcgctcgc gcagtccgac gcgaactgcg agggcgggtt ctgcgcgctc
     2821 acgaacatca agaacgtgga gttcgcctcg aacgccgccg agctcccgga cttcctcaat
     2881 gcgctctaca tgatctgcat cggactcgcg gccgtcatcg gcgtgctgca gatcatgcgt
     2941 gccggcgtca cgtggatgac cgctgccggc agccacgaga agatcgggga tgcgaagaag
     3001 ctggtccgcg atacgatcat cggactcgtc ctcgtcctcg ccccgacgat cgtgttcggg
     3061 atcatcaatc cgaagattct ctcgctcgag atcggctgcc tcgaggggct caagaagggc
     3121 gcggtggact gcgcgggtaa tgtgaaaacg ccggaggcga cgcctggatc gagcggcccg
     3181 gtctgcggcg attaccgcaa gaagcaggtc gtgccgggga acatggtctg tagcgcgaag
     3241 aatgggtaca gcaagatcaa caacgactgc tgcggcgacg tccccgaagg ctcccagtgc
     3301 tgcggcaatc cgtacggtcc ggacaacccg ccgccgtcga tgatcccgaa tacgatcggg
     3361 accgataagc ccgagccgaa ccctgcctgc agggtgacgt acaaggacgg gaacctgcta
     3421 ccggacggcg accaggcgca gcagttctgc tgcgattacc aggacgcgta cggcattcag
     3481 tgcacggcac tcaagaatcc gcggggcgcg ggcctctact gttcctgcca atagctatgc
     3541 gacgcatcct catcatcatc gcggccgtca tcctccttat catccttatc gtgggcgcgt
     3601 acttcctctt cttccgcgga ggtggagggc tcatcgtcgg tccggacccg ttcggcccgg
     3661 caggggaccg ggacccgggc cttgcgccgg tcgtggatac gggggtcgag gtgccgggcg
     3721 cgggcacctc gatcggcccg cgcctactgc agctcaccga tcgcgcggtc gcgaagggca
     3781 ccgtctccct gtatacgccg ccgaggaccg tgacggtcgc gagctcgacc tccacctcga
     3841 ccccgcctgt ggtcgagacg acccccgcgg aggtcgaagt ccgctacatc gaccgccaga
     3901 gcggcaatgt gtattccttc aaggtgcatg agcgggtact gacccgtctt tcgaacaaaa
     3961 ccctgccggg cgtcatcgag gcgtcgtgga cgcgcgatgg ctcgcgggcc ttcgtgcgct
     4021 tcctcgagcg ggacgttgcg ggcgccgagc gcgtaagcac gtacctcctg cccgcgaatg
     4081 gggaaggggg cttcttcctc gagcagggcc tcgcgcaggt ggaggtcgcg agcaccacgg
     4141 tgctgaccct gctttcgacc gacaccgggt cggtggcgag cgcgatgaac gcggacggca
     4201 cggggacccg cacgctcttc tcctcggtcc tctcctcgct ccgggtcgcg tacctcggca
     4261 cgagctacct cgcgcaaacg aaaccgaccg cgcacctcga cggctacgcc ttcatcgtga
     4321 gcggcggcac cttcacgcgg ctcctcggcc cgctgcgcgg cctttcaacg ctgccgtcgc
     4381 cctcgggaag gaacgtgctc tatagctacg tggatcgcgg gaaactatat acgcaggtgc
     4441 tcgatgtctc ggcgcgcacc acgctgccgc tcccgcttgc caccctgccg gagaagtgcg
     4501 tctggacgcc ggatgggagc gccctttact gcggcgtgcc gacgtccgtc gcggggctgc
     4561 tcccggacga ctggtaccag ggcgcgcgtc ccttctcgga ccgtgtctgg cgcatcgata
     4621 tggcgacgcg ggtggcgacg cttgtcttcg acccccgcca gatcgcgcag gtttcgatcg
     4681 acgcgcaggc gctctcgctc gatagcgcaa gcgacgtcct cgtattcatg aacaaatcgg
     4741 acgg
//
LOCUS       DASPKG010000035         3689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1548226, whole genome shotgun sequence.
ACCESSION   DASPKG010000035 DASPKG010000000
VERSION     DASPKG010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548226"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /gene="nth"
                     /locus_tag="VEA36_01260"
     CDS             <1..551
                     /gene="nth"
                     /locus_tag="VEA36_01260"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYF28976.1"
                     /translation="ASVVLSAQCTDKKVNAVTDTLWKKYRTVDAFAEADLAAFTKEIS
                     SLTFFQNKAKHIIAAAKLVNEKFGGEVPKTASELIELPGVAYKTAHVVLGELYNVWEG
                     IPTDTHVRRFALRFDLVDSTDLTKISKELEALVPKEDWRYVNNGLVLYGRYVCPAIPH
                     PCEEHPLTKLWPPAANVWPKAK"
     gene            831..1523
                     /locus_tag="VEA36_01265"
     CDS             831..1523
                     /locus_tag="VEA36_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HYF28977.1"
                     /translation="MEHQKPVAVFDIDGTLFRSTLLIELVEELIAREIFPPEARKSYE
                     TSQLEWLNRKGEYEPFIRKVVQAFGKYAKGVPYGEVADVAGEIIEGKHERTYRYTRDL
                     LDDLKKKGYYLLAISHSPKFIVDGFAYELGFDKSYGTFYETGATNRFTGNIEDEHLIM
                     NKSAVLKRAVEKENLTFRSSFGVGDTESDISMLTMVENPIAFNPNRLLYRHAKKHGWP
                     VVVERKDVIYEL"
     gene            1520..2308
                     /locus_tag="VEA36_01270"
     CDS             1520..2308
                     /locus_tag="VEA36_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HYF28978.1"
                     /translation="MMNPHLPQDGAYLHALAEKIPAKTAFADIRFEYLTELFTSVGIA
                     EDPYSYVNLDGNNAEAIDCILTQSFEAVQIACTNPAVQEQLHRLWDYLAEEDELAKVD
                     LIFVFGGIELTRMIEGVRLLKLGYAEKLLFSGKHASYVHEADLSEAEFYAQEAEKLGV
                     GRDQLILETEAVNTPENVINSVALLHRMQWMPEKVIIVTSPYHLRRAYLTSQAVADWH
                     PQLIRRGAPSTNHARDLYFKDQRTWSLTVLEYLKIYGGRVMKHF"
     gene            2340..2900
                     /locus_tag="VEA36_01275"
     CDS             2340..2900
                     /locus_tag="VEA36_01275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYF28979.1"
                     /translation="MTREARILLIGSGIWYVGEGLFGPLLAVFSEEIGGTILDLSWAW
                     AAYLIVYGVLSIVFGRLSDTSIDKGKLMVFGYALNAVFTFAYLLVTTPTGLFIVQAGL
                     GVAAAMATPTWDALFDESSEASLDGTSWGLADGTANIITGITIVIGGLIVSYLSFQAL
                     FVLMGTIQVIATIYQARILTVRRRQT"
     gene            2897..3238
                     /locus_tag="VEA36_01280"
     CDS             2897..3238
                     /locus_tag="VEA36_01280"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYF28980.1"
                     /translation="MRIIRSTEARLFNGGPSFQSHEYDTHEPDLNVAKVMITGRVPAT
                     GTMRNTKVKEIVYVEAGTGTITIGGKTHEIGAGDVIFYEPFEEVAWEGELTLIIACTP
                     AWSLEQHEMLP"
     gene            3253..3501
                     /locus_tag="VEA36_01285"
     CDS             3253..3501
                     /locus_tag="VEA36_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HYF28981.1"
                     /translation="MQKKLYRSREHRVLMGLCGGVAAYFAVDPNLVRLIYMVLTVITG
                     VFPGIIAYLLGIFIVPLEPTVERMETIIVDDGTDPTAI"
     gene            3473..>3689
                     /locus_tag="VEA36_01290"
     CDS             3473..>3689
                     /locus_tag="VEA36_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HYF28982.1"
                     /translation="MTGLTPQRFRNLVWRHYELHGRHDLPWRKTDDPYRVLVSEVMLQ
                     QTQVERVVPYYRDFLRQFPTLKKLAAAP"
BASE COUNT          772 a         1161 c         1047 g          709 t
ORIGIN      
        1 tcgcctcggt cgtgctgtct gcccagtgca cggacaagaa ggtgaacgcg gtcacggaca
       61 cgctctggaa gaagtaccgg acggtggatg ccttcgcgga ggcggacctt gccgccttca
      121 ccaaggagat ctcctcgctg accttcttcc agaacaaagc caagcacatt atcgcggcgg
      181 cgaagctggt caacgagaag ttcggcgggg aagtcccgaa gaccgcctcc gagctcatcg
      241 aattgccggg ggtcgcgtac aagaccgcgc acgtcgtgct cggcgagctc tacaacgtct
      301 gggagggcat cccaacggac acgcacgtgc ggcgcttcgc gctgcggttc gatctcgtgg
      361 actccaccga cctcacgaag atctcgaagg agctcgaggc cttggtcccc aaggaggact
      421 ggcggtacgt caataacggg ctcgtgctct atggccgcta cgtctgtccc gccatcccgc
      481 acccctgcga ggagcatccc ttgaccaagc tctggccgcc ggcggcgaat gtgtggccca
      541 aggcgaaata gttgacagtc gttctatgca ggactaagct caactctggt gccccccaac
      601 gggcaccagt cggcggtctc ggctctccat gtgggcgagc gtgtgttttt cctccggggg
      661 aaggttgcaa ccatccacca gcgcgcgtcc tcgcgacgta ccgaagtggt aggcactgcg
      721 accaaagcta acaggcctgc actccctcac tccctggatc cgagaccccg actccctaag
      781 gcgcttcgtg cgcctttttt atttccctcc cgaagcggta ccatttaccc atggagcacc
      841 agaagcccgt ggcggtcttc gacatcgacg gcaccctctt ccgctcgacg ctcttgatcg
      901 agctcgtcga ggaattgatc gcccgggaga tcttcccgcc cgaggcccgc aaatcctacg
      961 agacctcgca actcgagtgg ctcaaccgca aaggggaata cgagcccttc atccggaagg
     1021 tcgtgcaggc cttcggcaag tacgccaagg gcgtgccgta cggggaggtc gcggacgtcg
     1081 ccggcgaaat catcgaaggg aagcacgagc gcacctaccg ctacacccgc gacctcctcg
     1141 atgacctcaa gaagaagggc tactacctcc tcgccatctc gcactccccg aagttcatcg
     1201 tggacgggtt cgcctacgag ctcgggttcg ataagtcgta cggcacgttc tacgagaccg
     1261 gcgccaccaa ccgcttcacg ggcaacatcg aggacgagca cctcatcatg aacaagtccg
     1321 cggtcctgaa gcgcgccgtc gagaaggaga acctcacctt ccggagctcc ttcggggtag
     1381 gggataccga gagcgacatt tccatgctga ccatggtcga gaaccccatc gccttcaacc
     1441 cgaaccggct cttgtaccgc catgccaaga aacatggctg gccggtggtg gtggaaagga
     1501 aggacgtgat ctacgagcta tgatgaatcc gcacctcccg caggatggtg cctacctgca
     1561 tgcgctcgcc gaaaagatcc ctgccaagac cgccttcgca gatatcagat ttgaatacct
     1621 gaccgaactc ttcacctcgg tagggatcgc ggaggatcct tactcatacg tgaacctcga
     1681 cgggaataat gcagaggcga tcgattgcat cctcacgcaa tcgttcgaag ctgtccagat
     1741 tgcatgcacc aatccagcgg ttcaggagca attacaccgt ctctgggatt atctcgctga
     1801 agaggatgaa cttgccaaag tcgatctcat tttcgtattc ggcggcatcg agcttacgcg
     1861 catgatcgaa ggtgtacggt tactgaaact cggctatgca gagaaactgc tgttttcagg
     1921 caaacacgcc tcgtatgtac atgaggctga cctgagtgaa gctgaattct acgcacagga
     1981 ggcagagaag ttgggagtgg gaagagatca gctcatactc gagaccgagg cagtcaatac
     2041 gcccgagaat gtcattaact cagtcgccct acttcatcgg atgcagtgga tgccggaaaa
     2101 ggtcatcata gtcacttctc cctatcatct ccgccgggcg tatctgacct cccaagcggt
     2161 agcagactgg cacccacagc ttatccgacg aggagcgccc tccactaatc atgcgcgaga
     2221 tctgtacttc aaggatcagc gtacctggtc gctcacggtg ctcgaatatc tgaagatata
     2281 cggcggccga gtcatgaagc acttttaaaa gaggaaatcc aagtgctaca ctcaataccg
     2341 tgacccgaga agcgcgcatc ctcctcatcg gcagcgggat atggtacgtg ggcgaagggc
     2401 tcttcggccc gctgcttgcc gtattctcgg aagagatcgg cggcaccatc ctcgacctct
     2461 cctgggcctg ggccgcgtac ctcatcgtat acggcgtgct ctcgatcgtg ttcgggcgcc
     2521 tctccgatac ctcgatcgac aaggggaagc tgatggtatt cggctatgcg ctgaacgccg
     2581 tgttcacctt cgcctatctc ctcgtgacca ccccgacggg cctcttcatc gtgcaggcgg
     2641 ggctcggcgt tgccgccgcg atggcgaccc cgacctggga cgccctgttc gatgagtcct
     2701 ccgaggcgtc gctcgacggc acctcctggg ggcttgccga cggcaccgcg aacatcatta
     2761 ccggcatcac gatcgtcatc ggcggcctca tcgtgagcta cctctcgttc caggcgctct
     2821 tcgtcctcat gggcacgatc caggtcatcg cgaccatcta ccaggcccgc atcctcaccg
     2881 tgcggaggag gcagacatga gaatcatccg cagtaccgaa gcaaggctct ttaacggcgg
     2941 cccgtcgttc caatcccacg aatacgacac gcacgagccg gacctcaacg tcgcgaaggt
     3001 gatgatcacc ggacgggttc ctgcgaccgg caccatgcgc aacacgaagg tgaaggagat
     3061 cgtgtacgtc gaggcgggaa cgggcaccat tacgatcggc gggaagaccc acgagatcgg
     3121 ggcaggggac gtcatcttct acgagccctt tgaggaggtc gcctgggagg gagagctgac
     3181 cctcatcatc gcctgcacgc ctgcctggtc cttggagcag cacgaaatgc tgccctagcg
     3241 tatactctcc ccatgcagaa gaaactctac cggagccggg agcatcgcgt cctcatgggg
     3301 ctctgcggtg gtgtggccgc gtatttcgca gtggacccga acctcgtccg gcttatctac
     3361 atggtactca cggttatcac cggcgttttc cccggaatca tcgcctacct gctcggtatc
     3421 ttcatcgttc cgttagagcc gacggtggaa cgcatggaga cgatcatcgt cgatgacggg
     3481 actgacccca cagcgattta ggaacctcgt ctggaggcat tacgagctcc acgggcgcca
     3541 cgatctcccc tggaggaaga cggacgaccc gtaccgcgtc ctcgtctccg aggtcatgct
     3601 ccagcagacg caggtggagc gggtcgtccc gtactaccgg gatttcctca ggcagtttcc
     3661 gacgctcaag aagcttgccg ctgcaccac
//
LOCUS       DASPKG010000036         5245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_65396, whole genome shotgun sequence.
ACCESSION   DASPKG010000036 DASPKG010000000
VERSION     DASPKG010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65396"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VEA36_01295"
     CDS             <1..1246
                     /locus_tag="VEA36_01295"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HYF28983.1"
                     /translation="CTEITLNTNDEETAVCTIGSLNFAKFVVNGKFDHELVAKVTRTA
                     VRMLDNVIDLNYYPTEDAKRGNMRHRPVGLGVRGYHDALYALNINFDTPEALAFADES
                     MEVVAYYTILASSELAKERGTYESYKGSKWDRGIFPQDTIDLLQEERGEEIKVKRGGK
                     LDWTPVREHVKQYGMRNSNTMAIAPTASTANLVGCIPCVEPIYKNIYVKSNKEGEFVV
                     VNKYLVEDLKQLGLWDTGMLNRIKYADGSIQHIDEIPQRLKDKYKEVFEIDGKWLVEA
                     AARRGKWMDQSQSLNIFFSGTSGRELSDIYFMAWQMGLKTTYYLRSLGASQVEKATVS
                     TAEYGSTHTRGVGGPMTAAVAAEAVVAAVESLEPVPVMAAAAPTQPTLGVPEGFSAQE
                     WQAKLARVAAGEESGVCESCEA"
     gene            1390..2424
                     /locus_tag="VEA36_01300"
     CDS             1390..2424
                     /locus_tag="VEA36_01300"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HYF28984.1"
                     /translation="MPINKGAGVVDINKRRIINGEDADVVQLYPMRHTFAWEAYMAGN
                     ANHWLPTEISMQRDIEQWKSPNVLSDDERRAFETVLGFFTTADSIAANNVVLGIYKHI
                     TSPECRIYLLRQGYEEAIHTHAYQYIVESLQMDEAKIFNMYRQVEAIYNKDNMVLSLN
                     EGIFDPNFKTGTLEADQKFVENLAVFSLIMEGIFFYSSFAVMFGFQRQNKLTGSAEQI
                     QYIMRDESQHLNFGINLINSIKEEQPEVWTPAFQQHLVDLVKRAVVLEYTFAQEVFPK
                     GIFGMNAAGFKQYIEHIADRRLARVGLPAQYNVDNPFPWMSEAVDLNKEKNFFETRVT
                     EYKTGGQLNW"
     gene            complement(2495..2986)
                     /locus_tag="VEA36_01305"
     CDS             complement(2495..2986)
                     /locus_tag="VEA36_01305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HYF28985.1"
                     /translation="MFEGKYVKKHKAWVRDKQMLDLRTVPADFFYHEREVWWTAIGVN
                     IGVEIDGKNEGFERPVLVVRKINADQFFGIPLSSNKKIGRLRIPIKNTWCAGTLYLNQ
                     LRVLSTKRMLRKMGTLDRHSFVDVKMRLVNYLSEEWLDTREYESSRTPTQGRGSRRPK
                     PRI"
     gene            complement(3011..3505)
                     /locus_tag="VEA36_01310"
     CDS             complement(3011..3505)
                     /locus_tag="VEA36_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HYF28986.1"
                     /translation="MMPVRGLMLIEILIVLGMLALLSTVVLVAVNPLRQFAQARNTQR
                     EANVSALLNAVGHRLADNRGIFEIASSTCSARLPISPTAISSSGGYDLRPCLVPAYLP
                     ELPFDPQEGTNTCTTETCSAAGEAYDTAYTISQDASTSRITVCAPLHAEPAITDSSEF
                     CLTR"
     gene            complement(3677..3952)
                     /locus_tag="VEA36_01315"
     CDS             complement(3677..3952)
                     /locus_tag="VEA36_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28987.1"
                     /translation="MKRNRKLWFRAKRFGWGWYPASWQGWAVTVAYTLLYVLSGLLFG
                     AFAPAVVAEGGSVLEGSVLFASLLVLITASLLAICYRYGERPRWRWG"
     gene            4133..4744
                     /locus_tag="VEA36_01320"
     CDS             4133..4744
                     /locus_tag="VEA36_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28988.1"
                     /translation="MQFIAIAVIGIFFLPASVGAPAHAQVTSAPAVATAAEDEPSPTK
                     VALEKRATQSYTVSMTAYNAVPAQTDADPFITASGAWTNPEIVAARSQDLAAELPFGT
                     VIKVERAAADSPRCQFGAVEHLIGYRVIADTMHSRWTKKIDILLDQTDTVPVAGAEMN
                     PAKAVGVCGGVTITVVGKIDIKEMPATQEELREMFETPALART"
     gene            4820..>5245
                     /locus_tag="VEA36_01325"
     CDS             4820..>5245
                     /locus_tag="VEA36_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28989.1"
                     /translation="MKKFFHHLGVVFAVLAGLALVVASFSPMFAAAAQAQTTDLEYSG
                     WIPYWAAKKGADDARKHIHQLDEVNPFTYTVKTNGQINDAGKMNASHWRKLKSAAKKE
                     GVRYVPTIMWSDGAKIHATLSDPALRVAHIQEIVALVEEH"
BASE COUNT         1195 a         1579 c         1494 g          977 t
ORIGIN      
        1 ctgcaccgag atcacgctga acaccaacga cgaggagacc gcagtctgca ccatcggatc
       61 gcttaacttc gcgaagttcg ttgtgaacgg caagttcgac cacgagctcg tggcgaaggt
      121 gacccgtacc gccgttcgca tgctcgataa cgtcatcgac ctcaactact acccgacgga
      181 ggacgctaag cgcggcaaca tgcggcaccg ccccgtgggg ctcggggtgc gcgggtacca
      241 tgacgcgctc tacgccctca acatcaactt cgataccccg gaggcgctcg cgttcgcgga
      301 cgaatccatg gaggtcgtcg cgtactacac catcctcgcg tcttccgagc tcgcgaaaga
      361 gcgcggcacc tacgagagct ataagggcag caaatgggac cgcggcatct tcccgcagga
      421 caccatcgat ctcctccagg aggagcgcgg cgaggagatc aaggtgaagc gcggcggcaa
      481 gctcgattgg acccccgtcc gcgagcacgt gaagcagtac ggcatgcgca actccaacac
      541 catggcgatc gccccgacgg catccaccgc gaatctcgtc ggctgcatcc cctgcgtgga
      601 gcccatctac aagaacatct acgtgaagtc gaacaaggag ggcgagttcg tcgtggtcaa
      661 caagtacctc gtcgaggacc tgaagcagct gggcctttgg gatacgggga tgctgaaccg
      721 catcaagtac gccgacggct cgatccagca catcgacgag atcccgcagc ggctgaagga
      781 caagtacaag gaggtgttcg agatcgacgg caaatggctc gtcgaggcgg cggcccgccg
      841 cggcaagtgg atggaccagt cccagtcgct caatatcttc ttctccggga cctccggccg
      901 cgagctctcc gatatctact tcatggcctg gcagatgggc ctcaagacca cctactacct
      961 gcgcagcctg ggggcatccc aagtggagaa ggcgaccgtt tcgaccgccg agtacggctc
     1021 gacccacacc cgcggcgtcg gcggccccat gaccgcagcg gtcgcggcag aagccgtcgt
     1081 tgcggcggtc gagagccttg agcctgtgcc ggttatggcc gcggcagcgc cgacgcagcc
     1141 aacgcttggc gttcccgagg gcttttctgc ccaagaatgg caggcgaagc tcgcccgcgt
     1201 ggcggcaggg gaggaaagcg gggtctgcga gagctgcgaa gcctaaccca gccgcataat
     1261 ctattgacac gagccggcaa atgccggctc gtgtcgcttt caacaggttg cggtaccgca
     1321 tgaagcgcgc gacaatgaga gtccgtcgag ggacccacga tccctgatta tcggtaatca
     1381 atacatcgta tgcctatcaa caaaggagcg ggagtcgttg acatcaacaa gcgccgcatc
     1441 atcaatgggg aagacgcgga cgtcgtccag ctctacccga tgcgccacac tttcgcctgg
     1501 gaagcgtaca tggccggtaa cgccaaccac tggctcccga ccgagatctc catgcagcgg
     1561 gacatcgagc agtggaagag cccgaacgtg ctctcggacg acgagcgcag ggcgttcgag
     1621 accgtgcttg gcttcttcac gaccgcggac tccatcgcgg cgaacaacgt ggtgctcggc
     1681 atctacaagc acatcacgag ccccgagtgc cggatatacc tcctgcggca ggggtacgag
     1741 gaggcgatcc acacgcatgc gtaccagtac atcgtggagt ccttgcagat ggacgaggcg
     1801 aagatcttca acatgtaccg gcaggtcgag gcgatctaca acaaggacaa catggtcctc
     1861 tccctcaacg agggcatctt cgatccgaac ttcaagacgg gcactctcga ggcggaccag
     1921 aaatttgtcg agaacctcgc ggtcttctcc ttgatcatgg agggcatctt cttctattcg
     1981 tcgtttgcgg tcatgttcgg cttccagcgc cagaacaagc tcacgggttc cgcggagcag
     2041 atccagtaca tcatgcgcga cgagtcgcag cacctcaatt tcggcattaa tctcattaat
     2101 agcatcaagg aggagcagcc ggaggtctgg acccctgcct tccagcagca cctcgtggat
     2161 ctcgtgaagc gcgccgtggt gctcgagtac accttcgcgc aggaggtctt cccgaagggc
     2221 attttcggca tgaacgcggc cgggttcaag cagtacatcg agcacatcgc ggaccgccgg
     2281 ctggcgcgcg tcgggctccc ggcccagtac aacgtggaca atccgttccc gtggatgagc
     2341 gaggcggttg acctcaacaa ggagaagaac ttcttcgaga cccgcgttac cgaatacaag
     2401 acgggcggtc agttgaattg gtaagataat tagctcggat agctagagct attcccgcta
     2461 ttacttgtaa attcaggatt gctctgtata ctagctatat tcgtggcttc ggcctccgag
     2521 aaccccggcc ttgcgtcggg gttcgactgc tttcatattc gcgagtatcc aaccattctt
     2581 ctgacagata gtttactagg cgcatcttta catcaacaaa agagtgacgg tccagcgttc
     2641 ccattttcct caacattcgc ttcgtactta aaacacgaag ctgattgagg taaagagtgc
     2701 cggcgcacca agtattctta ataggaatac gcaaccttcc gattttctta ttggacgaga
     2761 gtgggattcc aaagaactga tccgcattga tcttacgcac gactagtact ggtcgttcaa
     2821 agccttcgtt cttgccatct atttcaacac ctatattcac gccaatcgcg gtccaccaga
     2881 cttcacgctc atgatagaag aaatctgctg gtacagtcct caggtcgagc atctgcttat
     2941 ctctcaccca ggctttatgt ttttttacat acttgccttc aaacattctt ttatcgaatt
     3001 acgcttagaa ttagcgagta aggcagaatt ccgatgagtc agtgattgct ggttcggcat
     3061 gtaagggcgc acagactgta atgcgagatg tcgatgcatc ctgcgatatc gtataagccg
     3121 tatcgtacgc ctccccggca gcggagcagg tctcggtagt acaggtattc gtgccttcct
     3181 gcggatcgaa tgggagctca ggcaggtaag caggaacgag acagggacgc agatcatagc
     3241 cgcccgaact gctaattgcg gtaggggaga taggaagcct tgcgctgcag gtgctgcttg
     3301 cgatctcgaa aatcccgcga ttatctgcca gacgatgccc gacggcgttc agaagcgcgg
     3361 aaacattcgc ctcgcgctgc gtgttgcgcg cctgggcgaa ctgcctgagc ggattcacag
     3421 cgaccagcac gaccgtcgag agaagcgcga gcatgccaag cacgatgagt atctcgatga
     3481 gcataagccc tcggactggc atcatagatg agtgactagt ggtatgacgc catcctaagg
     3541 actcgagata aaatagttat aaagaaaaat tcaaatttct cttaaggtag cgaacatgat
     3601 catccttgtt cgctgagaat acgagaagaa gttcagggaa agatggaggg ggaataaaga
     3661 aaacctgtat tgcgcgctac ccccaccgcc acctcggccg ttccccgtac cgatagcaga
     3721 tcgcgagcag ggaggcggtg atgagaacga gcagggaggc gaagagcacc gatccctcga
     3781 gcacagagcc accctcggcg accaccgcag gcgcgaacgc accgaagaga agccccgaaa
     3841 ggacatagag gagggtgtag gcgaccgtca cggcccatcc ctgccaggag gcggggtacc
     3901 agccccaccc gaagcgcttc gcacggaacc acagcttccg gtttcttttc atatatggcg
     3961 taattacgcg gtactttagc accattgacc aggggatact aggccttgac cccctgaaga
     4021 ccatatgaag cccatccggc caaccccttc cacagcactt gacaaaacct gtcaagtctg
     4081 ctagaatggc ctcactgggc cgtttcagag caatctggaa tacccggtga atatgcaatt
     4141 catcgctatt gccgtaatcg gcattttctt tttaccggcg agcgttgggg ccccggcaca
     4201 tgcgcaagta accagcgcac ctgccgtcgc caccgcagcc gaggacgagc cttcccctac
     4261 gaaggtcgcc ctcgagaagc gagcgaccca aagctatacg gtaagcatga cggcctacaa
     4321 cgccgtcccc gcgcagaccg acgcggatcc cttcatcacc gcatccggtg catggacgaa
     4381 tcccgagatc gtcgcagcgc gctcccagga tctggctgcc gaactcccgt tcggcacggt
     4441 gatcaaggtc gagcgcgcag ccgcggactc gccccgctgc cagttcggcg ccgtggagca
     4501 tctcatcggc taccgcgtca tcgcggacac catgcacagc cgctggacga agaagatcga
     4561 catcctactc gaccagacgg ataccgtgcc ggtggctggc gctgagatga accccgccaa
     4621 ggcggtcggc gtttgcggcg gcgtgaccat cacggtcgtg gggaagatcg acatcaagga
     4681 gatgcccgcc acccaggagg agctccgcga gatgttcgag acccccgcgc tcgcgcgcac
     4741 gtaaaccaca tacgaagata gaacgaggcc gcagacagga tctgcggcct cgttcgtctt
     4801 ttggtatact gtgatcccta tgaagaaatt cttccaccac ctcggcgtcg tcttcgcagt
     4861 cctcgccggc ctcgcgctcg tcgtcgcctc gttctctccc atgttcgccg cggcagcgca
     4921 ggcgcaaacc accgacctcg agtactccgg ctggatcccg tactgggcgg ccaagaaggg
     4981 cgcggacgac gcccgcaagc acatccatca gctcgatgag gtgaatccgt tcacgtatac
     5041 cgtgaagacg aacgggcaga tcaacgatgc cgggaagatg aatgcatcgc actggcgcaa
     5101 actgaagagc gccgcgaaga aggagggggt gcgctacgtc ccgaccatca tgtggagcga
     5161 cggcgcgaag atccacgcga ccctctcgga ccccgcgctg cgcgtcgccc acatccagga
     5221 gatcgtcgcg ctcgtcgagg agcat
//
LOCUS       DASPKG010000037         8132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1013518, whole genome shotgun sequence.
ACCESSION   DASPKG010000037 DASPKG010000000
VERSION     DASPKG010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013518"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VEA36_01330"
     CDS             <1..600
                     /locus_tag="VEA36_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28990.1"
                     /translation="LIVLALLIVGAAAYAFSTRKALSPAEPVNEAPGAVAAMRYTNFE
                     HDFRFTYPEGYVLTEAERGDAHRGHYQVMLVAEENAVPPENGEGPTAITVDVYQNDID
                     QQPVMNWITTTNIANFKLGDGKLAERRVGGEAAYTNTWSGLYEGKTTTFAHGDDIIAV
                     SVTWLTPEDEILEAYEHVLDSFEFGQFDPPVLNEDATPQ"
     gene            602..907
                     /locus_tag="VEA36_01335"
     CDS             602..907
                     /locus_tag="VEA36_01335"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HYF28991.1"
                     /translation="MNPFTQKIYELTRRIPKGKVATYAQLAKLAGNAKAARAVGAAMR
                     SNPDIPKTPCHRVVASNGALTGYSAGHGIPTKRKMLEAEGVAFRGLRVDLAASQWKG"
     gene            complement(904..1650)
                     /locus_tag="VEA36_01340"
     CDS             complement(904..1650)
                     /locus_tag="VEA36_01340"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HYF28992.1"
                     /translation="MKRPLKKRPEPKAPGFPMRLNQYLAHKGHATRRGADELIEKGVV
                     YVNGTKARLGDKVNEKDTVEVRVKRPRSYVYLAFHKPKGMDTHKEPTGTTDVISSLPS
                     DLKRLKLFPVGRLDKNSRGLILLTNDGRVTDRLLNPEHEHEKVYEVMTKKPLRESAKE
                     KLEQGVVIEGYRTKPAKVTILSENRFRIALTEGKSHQVRRMVVALFNEVADLKRTRIG
                     NIAIEPLKPGEYRRLSGKELETFLDSLGLS"
     gene            1680..2093
                     /locus_tag="VEA36_01345"
     CDS             1680..2093
                     /locus_tag="VEA36_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYF28993.1"
                     /translation="MEERPKVGIGVLVLKEGRILLGERLASHGAGSWQIPGGHLEQGD
                     TFEETALRELQEETGLTDAAVERMICVINDRVYGKHFVTIGMLLSWRSGEPYDAEPGK
                     SRNWSWYLPEELPENMFLPSKNVIDCWRAGTIYTP"
     gene            2097..2330
                     /locus_tag="VEA36_01350"
     CDS             2097..2330
                     /locus_tag="VEA36_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HYF28994.1"
                     /translation="MEPIPGIYYHFKDPDKLYEVIGIAFNTETGEDMVVYKPLYEGAP
                     APLCVRPRSMFIEEVDRPDHNYRGPRFVYVREA"
     gene            2405..2905
                     /locus_tag="VEA36_01355"
     CDS             2405..2905
                     /locus_tag="VEA36_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYF28995.1"
                     /translation="MDQEDAKAYFLETYDAYGSDIYRFCLMKVSSREVAEDLTQEVFL
                     RFWQTVREGTELRNARALLYTVARNLVIDWYRKKKETSLDLLNEKGIDFAGEDFKKIT
                     EHAEHQEVLRVIDTLDGPSKEALMLRFVDGLSPRDIASISGETPNAISVRLNRAIKKV
                     QQQLQP"
     gene            2902..3819
                     /locus_tag="VEA36_01360"
     CDS             2902..3819
                     /locus_tag="VEA36_01360"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HYF28996.1"
                     /translation="MNHRFINFIRGLREAKLSDASFARIRDALASYADLHTVPNGALS
                     HTPILSPLASFFRMKGTLYAGALVLVVMVAGGGAATGAERAVPGDVLYALKINVNEKV
                     AATLADTPMEKAELSAKLATRRADEAIALLAEGRLDETTASYLDAQVAQNVEASSVAA
                     ATLESEGDIAASLMVRTDLEEELTKRAASLAPPAAEAADTATMMMAAKAAPADPGTQL
                     AEGLRAQAHRVAEARNHTAANILPGIADVGAELSAPAADTAGSATLMLDAGTTTEEMP
                     VRAKIAPTPSNAWPQEGEANRALWLPEGN"
     gene            3851..5422
                     /gene="lysS"
                     /locus_tag="VEA36_01365"
     CDS             3851..5422
                     /gene="lysS"
                     /locus_tag="VEA36_01365"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HYF28997.1"
                     /translation="MFWADRIAKDIEATKEGTKRPLLIRDEKTLSGRVHIGSMRGVAI
                     HGIVSEVLTEYGIDNRFLWEHNDFDPFDTVPAYLDRAAFEEHLGKPLYTVPSPEPGFE
                     NYAEYYADEFARVHQKAGFTPTYYRLYADLYATGDMDPYIRTALTHAADIRRIQKEVS
                     GSVKDENWLPISVICERCGKMMTTRAYDFDGETVGYACDRSPDECVPCGYEGRVAPWK
                     GAAKLFWKADWAAKWSALGVDIEGAGKDHSTRGGSRDVAKHIATEVFKRESPYDIPYE
                     FFLVGGKKMSSSKGRGSSAKDMSDLFPPQLLRLVLLGKDINQQIDVDPEGDSIPRLYD
                     WYDDLAESVRNGVADDFTRLYALCQLPAQRAELSAPWQMRFSQVAFIVQMPHLDLAAE
                     ASAAKGAPLTAEERALLEERAGYARFWLATYAPERYRYELQDSVPTGLALTDRQRDAL
                     RTLAGFFDTDRTGEEVHHRLHELKAEVPIEPKELFAALYRIFLNRDSGPKAGWFLSVL
                     PREFVVNRLREASGT"
     gene            5482..5901
                     /locus_tag="VEA36_01370"
     CDS             5482..5901
                     /locus_tag="VEA36_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28998.1"
                     /translation="MWKGLVLLIIFVIGSVVLAIYAQDKITELPPVSEIPQTATVALA
                     PVDVPLVVEGTIVFDDAPARDVQPFVLYGEPRADGTAAVKTKRLVFDAQTVCMVGDVP
                     CVFSAGGGYPVSPGDHVRVAGIAEADVIYVRSIQILA"
     gene            5925..6596
                     /locus_tag="VEA36_01375"
     CDS             5925..6596
                     /locus_tag="VEA36_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF28999.1"
                     /translation="MPWRFHRIWIVAVLLVSGLALAWLIAKTPLPQPAATMQPVATPT
                     PDLTGSAIYTNGPYGFAITYPERAVVADAFASAHLPSSWRVNAIGEGNPIVSITTYAT
                     ASEHAYPRHFEAVVRIGASTDVREVRECLRPTADRGETALPDATFGGATWKVFSFGDA
                     GMQRYVRGESYRTVHEGACVAIERITAGSRYREGAHPDDVSEAVLEDAYGALDQVVKS
                     FVFAR"
     gene            6598..7101
                     /locus_tag="VEA36_01380"
     CDS             6598..7101
                     /locus_tag="VEA36_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HYF29000.1"
                     /translation="MQDARQGSLYVRNIIFGIEDSLAATVGLLSGIAAEQVPHETILL
                     TGFVYIFVEAFSMGIGSFLSEESAQEYEKKRTMTSKRAALGGLVMFLSCIVGGLVPIL
                     PYLLLPPEYTLAGSITLSLIALSILGLVHARVSKRRAWPRVVRMVALGGTAILVGVAV
                     GKLFGIA"
     gene            7163..7492
                     /locus_tag="VEA36_01385"
     CDS             7163..7492
                     /locus_tag="VEA36_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HYF29001.1"
                     /translation="MFLIHWLLTAVAILIAAYLIPGIDVTLVGSLVLAVVLGLINAFL
                     KPLIGIITLPINILTLGLFSLVVNALLIMLAGAFVPGFTVDGFWPAFFFSILVSLVTA
                     LFGAWRR"
     gene            complement(7563..7856)
                     /locus_tag="VEA36_01390"
     CDS             complement(7563..7856)
                     /locus_tag="VEA36_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29002.1"
                     /translation="MFDAPLDTAQERVAAGAALLDAQAPGWRDRIDVDRLSIQSCGRC
                     MLAQLYGNYARGVKHLGLDANGTTVRNGFNAGPGVSFSELDRAWKAQILTPAR"
BASE COUNT         1459 a         2636 c         2579 g         1458 t
ORIGIN      
        1 ctcatcgtgc ttgcgctcct tatcgtgggc gctgcagcct atgcattctc gaccaggaaa
       61 gcgctctcgc cggccgagcc ggttaacgag gcgcctggcg cggtcgccgc gatgcgctac
      121 accaacttcg agcacgactt ccgcttcacg tatccggaag ggtacgtcct caccgaggcg
      181 gagcgcggcg acgcacaccg cggccactac caggtcatgc tcgttgccga ggagaacgcc
      241 gtcccgcccg agaacggcga gggtccgacc gcgatcacgg tggacgtcta ccagaacgat
      301 atcgatcagc agccggtgat gaactggatc acgaccacca atatcgcgaa cttcaagctc
      361 ggggatggaa agctcgcgga gcgcagggtc ggcggcgagg ccgcatacac caacacctgg
      421 tccgggctct acgagggtaa gacgaccacc ttcgcgcatg gggacgacat catcgccgtc
      481 tccgtgacct ggctcacccc tgaggacgag atcctcgagg cgtacgaaca tgtgctcgat
      541 tccttcgagt tcgggcagtt tgacccgcca gtcttgaacg aggacgccac cccgcaatag
      601 gatgaatccg ttcacgcaga aaatctacga gctcacccgc cgcatcccga aagggaaggt
      661 ggccacctac gcgcagcttg cgaagcttgc ggggaacgcg aaggctgcgc gggcggtagg
      721 cgccgcgatg cgcagcaatc ccgacatccc gaagacgccc tgccatcggg tggtggcgtc
      781 gaacggcgcg ctcaccggat acagcgccgg gcacggcatc ccgaccaagc ggaagatgct
      841 cgaagcagag ggcgtggcgt tcaggggcct gcgggtggat ctcgccgcga gccagtggaa
      901 gggctagctg agcccaaggc tatctaaaaa ggtctcaagc tccttgcccg agagcctccg
      961 gtattcgccg ggcttcaagg gctcgatcgc gatgttcccg atgcgggtgc gcttgaggtc
     1021 cgctacctca ttgaagagcg cgaccaccat gcggcgcacc tggtgcgatt tcccttcggt
     1081 gagcgcgatc cggaagcggt tctcggagag gatggtcacc ttggcgggct tcgtgcggta
     1141 cccctcgatc acgacgccct gctcgagctt ctccttcgcg ctctcgcgaa gcggcttttt
     1201 cgtcatcacc tcgtacacct tctcgtgctc gtgctcgggg ttgaggaggc ggtccgtgac
     1261 gcggccgtcg ttggtgagca ggatgagccc gcgggagttc ttatcgaggc gcccgacggg
     1321 gaagagcttg aggcgcttca ggtccgacgg tagcgaactg atcacgtccg tggtgccggt
     1381 cggctcctta tgggtgtcca tgcccttggg cttatggaag gcgaggtata cgtagctgcg
     1441 cgggcgcttc acgcgcactt cgaccgtatc cttctcgttc accttgtcgc cgagcctggc
     1501 cttcgttccg ttcacataca cgacaccctt ctcgatgagc tcatcggcgc cgcggcgggt
     1561 ggcgtggccc ttatgcgcga ggtactggtt gaggcgcatg gggaagccgg gagctttggg
     1621 ttccggtcgc ttctttagag gccgcttcat ctattcaccc tatagtacta tctggagtaa
     1681 tggaagaacg gcccaaagtc ggcatcggcg tgctcgttct taaggaagga aggatcctgc
     1741 tcggcgagcg gctcgcgagc cacggcgcgg ggtcgtggca gatccccggc gggcacctcg
     1801 agcagggcga tacctttgag gagaccgcct tgcgcgaact tcaggaggag accgggctca
     1861 ccgatgccgc agtcgagcgg atgatctgcg tcatcaacga ccgcgtatac ggaaagcact
     1921 tcgtcacgat cggcatgctc ctttcctgga ggtccggcga gccctacgat gcggagcccg
     1981 ggaaatcccg caactggagc tggtacctgc ccgaagagct tccagagaat atgttcctgc
     2041 cgagcaagaa cgtgatcgat tgctggcgcg caggcacgat atatactccc tgatatatgg
     2101 aacccatccc cggcatctac taccacttca aggaccccga taagctctac gaggtcatcg
     2161 gcatcgcctt caatacggag acgggggagg acatggtcgt gtacaagccc ttgtacgagg
     2221 gtgcgcccgc gccgctctgc gtgcggccgc gctccatgtt catcgaggag gtcgaccggc
     2281 ccgaccataa ctaccgtggc ccgcgcttcg tatacgtgcg cgaagcctag ggaatacagg
     2341 ctctaagcgg tatcctaggg aaacggccgc ccgtaagagg gtatcggccg atacgtcatt
     2401 actcatggac caggaggacg ccaaagctta tttcctcgag acctacgatg cctacggcag
     2461 cgatatctac cgcttctgcc tcatgaaggt atcgagccgg gaggtcgcgg aggacctcac
     2521 ccaggaggtg ttcctccggt tctggcagac ggtccgtgag ggcaccgagc tcaggaacgc
     2581 ccgggcgctc ctgtatacgg tcgcgcggaa cctggtcatc gactggtacc gcaagaagaa
     2641 ggagacctcc ttagacctgc tcaatgagaa gggcatcgac ttcgccggcg aggacttcaa
     2701 gaagatcacc gagcacgccg agcaccagga ggtgctgcgc gtgatcgata ccttggatgg
     2761 tccctcgaag gaggccctca tgctccgctt cgtcgatggg ctttccccgc gcgacatcgc
     2821 cagcatatcg ggcgagaccc cgaacgccat ctcggtccgc ctcaaccgcg cgatcaagaa
     2881 ggtgcagcag caactacaac catgaaccat cgcttcatca atttcatacg cggcctgcgc
     2941 gaggcgaagc tctcggatgc gtcctttgcg cgcatccggg atgcgctcgc ctcctacgcc
     3001 gacctccaca ccgttccaaa cggggcgctt tcgcacaccc cgatcctctc cccgcttgcc
     3061 tcgttcttcc gcatgaaggg cacgctctac gcgggcgcgc tcgtgctcgt cgtcatggtc
     3121 gcgggcgggg gcgcggcgac cggcgccgag cgcgcggtcc cgggcgatgt cctctacgcg
     3181 ctcaagatca atgtgaacga gaaggtggcg gcgaccctcg cggacacccc gatggagaag
     3241 gcggagctct ccgcgaagct cgccacgcgg cgcgccgacg aagcgatcgc gctccttgcc
     3301 gagggcaggc tcgacgagac gaccgcgagc tacctcgatg cgcaggtcgc ccagaacgtc
     3361 gaggcctcaa gcgtcgccgc agcgaccctt gagtccgaag gagacatcgc ggcatcgctt
     3421 atggtgcgta ccgatctcga ggaggagctc accaagcgcg ccgcgtcact tgcgcccccg
     3481 gcagcagagg ctgccgatac cgcgaccatg atgatggcgg cgaaggcagc gcctgcagac
     3541 ccaggcaccc agcttgccga ggggctacgc gcccaggcgc atcgcgtcgc tgaggcgcgc
     3601 aaccatacgg cagcgaacat cctgcccggc atcgcggacg tgggcgctga gctttccgcc
     3661 ccggctgcgg ataccgcagg ctccgccacc ctcatgctcg atgcaggcac cacgaccgag
     3721 gagatgccgg tgcgcgcgaa gatcgcaccg accccgagca acgcctggcc gcaggagggc
     3781 gaagcgaacc gggcgctctg gctcccagaa ggcaactaag ccaaggctgc aggcgtggta
     3841 ggatgcctgc atgttctggg cagaccgcat cgcgaaggac atagaggcga cgaaggaggg
     3901 gacaaaacgt ccgctcctta tccgcgatga gaagacgctg tcgggtaggg tgcacatcgg
     3961 ctcgatgcgc ggcgtcgcga tccacggaat cgtaagcgaa gtgctcaccg aatacgggat
     4021 cgataaccgc ttcctctggg agcataacga tttcgacccc tttgatactg ttcctgcgta
     4081 tctcgatcgc gccgcatttg aagagcatct gggtaagccg ttgtataccg tcccgtcgcc
     4141 ggagccaggt ttcgaaaact acgcggagta ttacgcggac gagttcgcac gtgtacacca
     4201 gaaggccggc tttacgccca catactaccg gctctacgcc gatctctatg cgaccgggga
     4261 catggatccg tacattcgga ccgcgcttac acatgccgcg gacatccgcc gcatccagaa
     4321 ggaggtatcg ggctccgtga aggacgaaaa ctggcttccc atctcagtta tctgcgaacg
     4381 gtgcggcaag atgatgacga cccgggccta cgactttgat ggcgagacgg tgggctacgc
     4441 ctgcgaccgt tcaccggacg agtgcgtacc ctgcggatac gaggggcgcg ttgcgccatg
     4501 gaagggtgcg gcaaaactct tctggaaagc ggactgggcc gcgaagtgga gcgcgcttgg
     4561 cgtggatatc gagggcgcgg gcaaggacca ctcgacacgc ggcggatccc gcgatgtcgc
     4621 gaagcatatt gcaacggaag tcttcaaacg ggagtcaccc tacgatatcc cctacgaatt
     4681 cttcctcgtc ggcggtaaga agatgtcatc ttcgaaaggt cgaggctcct ccgctaaaga
     4741 catgagcgac ctcttccctc cgcagctgct gcgcctcgtg ctgctcggga aggacatcaa
     4801 ccagcagatc gacgtcgacc cggaggggga ttccatcccg cgcctctacg attggtacga
     4861 cgatctcgcg gagagcgtgc gcaacggcgt cgcggacgac ttcacccgtc tctacgcgct
     4921 ctgccagctt ccggcgcagc gcgcggaact atccgcgccc tggcagatgc gcttctcgca
     4981 ggtcgcgttc atcgtgcaga tgccgcacct cgatctcgct gcggaggcca gtgccgccaa
     5041 aggcgcgccg ctcaccgcgg aagagcgggc gctccttgag gagcgagcgg ggtacgcgcg
     5101 cttctggctt gcgacctacg cgcccgagcg gtatcgctac gagctccagg attcggtgcc
     5161 tacgggcctt gcgcttacgg accggcagcg agatgcgctc cggaccctcg ccggattttt
     5221 cgatacggac cgcaccggcg aggaggtgca ccatcgcctg catgagctga aggccgaggt
     5281 accgatcgag ccgaaggagc tctttgccgc gctgtaccgc atcttcctga accgggattc
     5341 gggccccaag gccggatggt tcctatcggt cctaccgcgt gaattcgtgg tgaaccgcct
     5401 ccgggaggcg tcaggaacct aggggccatc cgtcgcagcg cgttcacgga acgtaaagcg
     5461 ccccttcgct atggtggagc gatgtggaaa ggattggtgc tcctcatcat attcgtgatc
     5521 gggtcggtgg tgctcgcgat ctacgcccag gacaagatca cggaactccc gcccgtctcc
     5581 gagattccgc agacggcgac cgtcgcgctc gcgccggttg acgtgccgct cgtggtcgag
     5641 gggacgatcg tcttcgatga cgcgcccgca cgtgacgtgc agcccttcgt cctgtacggg
     5701 gagcccaggg cggacggcac cgcggcggtg aaaaccaagc gcctcgtctt cgatgcgcag
     5761 acggtctgca tggtggggga cgtcccctgc gtcttctccg caggcggcgg gtatccggtc
     5821 tcgcccgggg accacgtgcg cgtcgccggc atcgcggagg cggacgtgat ctacgtgcgc
     5881 agcatccaga ttctcgcata gcctgcgtgc tatactccgg ccgtatgccc tggcgcttcc
     5941 accgcatctg gatcgttgcg gtactcctcg tgagcggtct ggcgctcgcg tggctcatcg
     6001 cgaagacgcc gctgccgcag ccggctgcga cgatgcagcc cgtcgccacg ccgacgcccg
     6061 acctcaccgg cagcgccatc tacacgaacg ggccctacgg gttcgcgatc acgtacccgg
     6121 agcgcgcagt ggtcgccgac gctttcgctt ccgcgcattt gccgtcttct tggcgcgtga
     6181 acgcgatcgg cgaagggaac cccatcgttt ccataacgac ctacgcgacc gcaagcgagc
     6241 acgcataccc gcgccacttc gaagcggtgg tgcgcatcgg cgcgagcacg gacgtgcggg
     6301 aggtcaggga gtgcctgcga ccgaccgctg accgcggcga gacggcgctt cccgatgcga
     6361 ccttcggcgg cgcgacctgg aaggtcttct ccttcgggga cgccggcatg caacggtacg
     6421 tgcgcggcga atcttaccgc accgtgcacg agggcgcctg tgtggcgatt gagcggatta
     6481 ccgcgggctc gcgctaccgc gaaggcgcgc atccggacga cgtttccgag gccgtgctcg
     6541 aagatgcgta cggcgcgctc gatcaggtgg tgaagagctt cgtgttcgcc cgctagcatg
     6601 caggacgcgc ggcaggggag cctctacgtg cgcaacatca tcttcgggat cgaggatagc
     6661 ctcgcggcca ccgtcggcct cctctcgggc atcgcggccg agcaggtgcc gcatgagacc
     6721 atcctcctca ccggcttcgt gtacatcttc gtcgaggcct tctccatggg catcgggagc
     6781 ttcctctccg aggagtccgc gcaggagtac gaaaagaagc gcaccatgac gagcaagcgg
     6841 gcggccctcg gcgggctcgt catgttcctc tcctgcatcg tgggcgggct cgtgcccatc
     6901 ctgccgtacc tgctgctgcc gcccgagtac acgctcgcgg gctccatcac gctctcgctc
     6961 atcgcgctgt ccatcctcgg gctcgtgcac gcgcgggtct ccaagcgacg ggcatggccg
     7021 cgcgtggtgc ggatggtcgc gctcggcggg accgcgatcc tcgtcggggt ggcggtcggc
     7081 aagctcttcg gcatcgccta gccttcacct tctgtttata cggccgcggt atgctgtata
     7141 cgttattccc taatcccgta ccatgtttct catccattgg cttctcacgg cggtcgcgat
     7201 cctcatcgcc gcctacctca tcccgggcat cgatgtgacc ctcgtggggt cgctcgtgct
     7261 cgcggtggtg ctcgggctca tcaacgcatt cctgaagccc ttgatcggga tcatcacgct
     7321 cccgatcaac atcctgacct tgggcctctt ctcgctcgtg gtgaatgcgc tcctcatcat
     7381 gctcgcgggt gccttcgtac ccggattcac ggtggacggg ttctggccgg ccttcttctt
     7441 ctccatcctc gtgtccttgg tgaccgcctt gttcggggcg tggcggcggt agagcaagca
     7501 atacaaagcg ggcgccggtc catgaccagc gcccgcttgc ctttgctgcc gcgccgacga
     7561 gctcaacgtg ccggcgtgag gatctgcgcc ttccaggccc ggtccagctc gctgaacgag
     7621 acgcccgggc cggcgttgaa gccgttgcgg accgtagtcc cgttcgcatc cagtcccagg
     7681 tgcttgacgc cgcgcgcgta gttcccgtag agctgggcca gcatgcagcg gccgcaggac
     7741 tggatgctga ggcgatccac gtcaatgcgg tcgcgccagc ccggcgcctg cgcgtccaga
     7801 agcgcagcgc ctgccgctac ccgctcctgc gcggtatcca gtggagcatc aaacatggca
     7861 atctcctgag gtgcgtgaaa gcccgtgctt tcaaccagga tcgaggatag cactgggcga
     7921 aactctatgc tagccgccgt agtgcgtcgg tggtatagtc ccgtcggact gtggatcagt
     7981 catcacattc aaacgcctta accgcgcagg aggtgcgcca tgagactcaa gcctatcgaa
     8041 ggcaagacca aaattctcaa gccaacacgg cgcggccggg attggctctg ggggccgcac
     8101 gtagcccgag agcacttcaa ggacgacacg ac
//
LOCUS       DASPKG010000038         4379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_609594, whole genome shotgun sequence.
ACCESSION   DASPKG010000038 DASPKG010000000
VERSION     DASPKG010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609594"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1868)
                     /locus_tag="VEA36_01395"
     CDS             complement(<1..1868)
                     /locus_tag="VEA36_01395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HYF29003.1"
                     /translation="MHPADPVEKHFTRLKPDQKKALGKLGIETVRDLLYHFPARYESA
                     GPTGTIAGAATGTDVTLYGTVRKPETRKAWKSRRPIAEAWLEDASGRIKLMWFNQPYI
                     AKTLADGMVVRATGKVTGEGSKRYLANPDLDRTPITSEEVHDSLFNEATAESERSLYA
                     IYPESKGISSLWFLHAQKRLFEGGLHEAVPDPIPKEILERYNLPSLSTALVWMHAPKK
                     QEDATVARKRFAFEEVFAIQLGMQREKLHAAREKALRVEVNRGALDDFIASFPFPPTS
                     AQSRSIESIVKDFEQSHPMRRLLEGDVGSGKTAVAAATAYMVATSRPPGRKAGTLQVA
                     YMCPTEILAKQHFSTFVSYFKDHPIPIGLITGSECRKFPSKIRREESTKISRAQFSKW
                     VANGEIPIVIGTHALVYKSLEFQNLAYAIIDEQHRFGKAHRQKLAKKDGISPHLLSMT
                     ATPIPRTLALTIYGDLDISLLDEMPAGRKPVITEIIDPNTREKAYERVRSELAEGRQA
                     YVICPRIDEPDPSKEMALQAKSVKAEAERLKQEVFPDASIDILHSKMTPAEKEKVMER
                     FERNEVQILVATSVVEVGVNVPNATVILIEGAERFGLAQLHQLRGRVGRGARQSAC"
     gene            complement(1947..2528)
                     /locus_tag="VEA36_01400"
     CDS             complement(1947..2528)
                     /locus_tag="VEA36_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29004.1"
                     /translation="MLLNTHCLEGHSIPRTEEIGMKTILSLSAAVAALLVSSGAYAET
                     KAETVELPDKSLHTTVTSKKGGIFAPGTATVTAYHCSPKCAVVKTEAATGPSLLAGAI
                     PSALYAVGDWASAKALRPNRHEVFVSNDSSSRSQGGNASAESQARGGQADAFVDQSYR
                     EDHYVDDVRIFDNVIVVPDVPSLPSATTSGEKG"
     gene            2604..4127
                     /locus_tag="VEA36_01405"
     CDS             2604..4127
                     /locus_tag="VEA36_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HYF29005.1"
                     /translation="MSRYARHWSVLIAVLLIASFLFAFPTSYLQAATAKIAGADALTV
                     AQLKAAYTEAAAGGEKVRILVVPGHEPMYGGAQYAGYYERELVIPLSEKLATELRSDP
                     NIEVILARDASGWNPTLERYFKRNLSSIKRFVETHQKEFRKLLRRGRVDENEAQAGHN
                     RAPADVAYRLYGITKWANENDVDLMIHVHLNDAGGRANDAPGAYTGLAIYVPYEEFGN
                     AEASKAIADKVFDHLTDIAAKSTLPIEDQGVVEDQELIALGSNNTSIAPSLLVEYGYI
                     YEPKFIDENVRNIVFSDYAYQTAKGVREFFGASAGGAHSTRVLPYTWTADIASSTASS
                     IPAYALQHALKEEGYYPPRPSSLINCPIDGRMGSCVTDALKAYQTAKGITPSGRLDAA
                     TRNALNAKYSAGSFIPEPTTIASVAAPAARCAPFTTTLKLNSTDAATKGQVSRLQKIL
                     AQDTTIYPEGRVTGFFGPATDKAVKAFQVKQGIAKPGSAGYGLVGPATGKALIAVCT"
     gene            4197..>4379
                     /locus_tag="VEA36_01410"
     CDS             4197..>4379
                     /locus_tag="VEA36_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29006.1"
                     /translation="MRVRISVLLLSFAFLFAPASASAATASELQAQIQALLAQLSVLQ
                     AQLNQAQGGTHSSRTGA"
BASE COUNT          775 a         1372 c         1402 g          830 t
ORIGIN      
        1 atgcaggcgc tctggcgggc gccgcggccc acgcggccgc gcagctggtg cagctgcgcg
       61 agcccgaagc gctccgcccc ttctataaga atgacggtcg cgttcgggac gttgacgccc
      121 acctccacca ccgaggtcgc cacgagtatc tgcacctcat ttctctcgaa gcgttccatg
      181 accttctcct tctcggcagg cgtcatcttg ctgtgcagga tgtcgatcga ggcgtcgggg
      241 aagacctcct gcttaaggcg ctccgcctcc gctttcacgg actttgcctg gagcgccatc
      301 tccttgctgg gatctggctc gtcgattcta gggcagatga cataggcttg gcggccttcc
      361 gcaagttccg agcgcacccg ctcgtatgcc ttctcgcggg tattggggtc gatgatctcc
      421 gtgatgacgg gcttgcggcc cgccggcatc tcatcaagga gcgagatgtc gaggtccccg
      481 tagatggtga gcgcgagcgt gcgggggatg ggggtcgcgg tcatggagag gaggtgcggg
      541 gagatgccgt ccttcttcgc gagcttctga cggtgcgcct tcccgaagcg gtgctgctcg
      601 tcgatgatgg cgtaggcgag gttctggaac tcgagcgatt tgtataccag ggcgtgcgtg
      661 ccgatgacga tcgggatctc gccgttcgct acccatttac tgaactgcgc gcgcgatatc
      721 ttcgtcgatt cctcgcggcg gatcttcgag gggaacttcc ggcattcgct gcccgtgatg
      781 aggccgatgg ggatcgggtg gtccttgaag tacgaaacga aggtcgagaa gtgctgcttg
      841 gcgaggatct cggtcgggca catgtaggcg acctggagcg ttcctgcctt cctgccgggc
      901 ggtcgcgagg tcgcgaccat gtatgcggtt gcggccgcaa ccgcggtctt gccgctcccc
      961 acgtctccct caaggaggcg gcgcatggga tgggactgct cgaagtcctt cacgatgctc
     1021 tcgatggagc gggattgcgc ggaagtcggc gggaagggga aggacgcgat gaaatcgtcg
     1081 agcgcgcctc ggttcacctc gacccgaagc gccttctccc tggcggcgtg gagcttctcc
     1141 cgctgcatgc cgagctggat ggcgaacact tcctcgaagg cgaaccgctt gcgcgccacc
     1201 gtcgcatcct cctgcttctt cggcgcatgc atccagacga gcgcggtgga aagggacggg
     1261 aggttatagc gctcgagtat ctccttggga atcggatcgg ggaccgcctc gtggagcccg
     1321 ccttcaaaga gccgcttctg cgcatgcaaa aaccacaacg aagagatgcc cttcgattcg
     1381 ggatagatgg cgtagaggga gcgctcgctc tcggcggtcg cctcgttgaa caaagagtcg
     1441 tgcacttcct cgctcgtgat gggggtgcga tcgaggtcgg gattcgcaag gtagcgcttc
     1501 gatccctcgc ccgtcacctt gcccgtcgcg cggaccacca tgccgtccgc taacgtcttc
     1561 gcgatgtacg gctggttgaa ccacatgagc ttgatgcgcc ccgacgcgtc ctcgagccac
     1621 gcctcggcga tgggcctcct actcttccag gccttgcggg tctcgggctt gcggacggtg
     1681 ccgtagagcg tgacgtcggt gccggtcgcc gctccggcga tggtgccggt ggggccggcg
     1741 gactcgtagc gggccgggaa gtggtagagg aggtcgcgca ctgtttcaat gccgagcttc
     1801 ccgagcgcct tcttctggtc gggcttgagg cgggtgaaat gcttctcgac ggggtccgca
     1861 ggatgcatgc ggtccagtat atagaaaaag tcggagcccg ctccaatatg cagagcgggc
     1921 ccgaagggta cttggtactg tggcgatcag cccttctcgc cggaggtcgt agccgaaggc
     1981 agcgacggga cgtcggggac cacgatcacg ttgtcgaaga tcctgacgtc gtccacatag
     2041 tggtcctccc tgtaggactg gtcgacgaag gcgtcggcct gaccgccgcg cgcctgcgac
     2101 tccgcggatg cgttgccgcc ctgcgagcgg gacgaggagt cgttcgagac gaacacctcg
     2161 tgccggttcg ggcggagcgc cttggcgctc gcccagtcgc cgacggcgta gagcgcgctc
     2221 gggatagcgc ccgcaagcag gctcgggccg gtggcggcct cggtcttcac gacggcgcac
     2281 ttcggactgc agtggtaggc ggtcacggtg gcggtgccgg gcgcgaagat accgcccttc
     2341 ttgctcgtca cggtcgtgtg caacgacttg tcgggcagct ccaccgtctc cgccttcgtc
     2401 tcggcgtagg cgccggacga aacgagcaac gcggctacgg ccgctgatag tgaaaggatc
     2461 gtcttcatgc ctatctcctc tgttctcggg atggaatgtc cctcaaggca gtgggtattt
     2521 aatagcatat tatgcaatat ttgtcaatat agccgcgtaa gaacacccat tcttcatcgt
     2581 cctatgacgt atagtctcta catatgtctc gatacgctcg tcattggtcg gtcctcatcg
     2641 cggtcctgtt gatcgcctcc ttcctctttg ccttcccgac ctcctacctc caggccgcga
     2701 ccgccaagat cgcgggcgca gacgcactca ccgttgcgca gctcaaggcc gcgtacaccg
     2761 aagcggccgc aggcggcgag aaggtccgca tcctcgtcgt gcccgggcac gagcccatgt
     2821 acggaggtgc gcagtacgcc ggctactacg aacgcgagct cgtgataccg ctctccgaga
     2881 agctcgcgac cgagctgcgg tccgatccga acatcgaggt gatcctcgcg cgcgatgcga
     2941 gcggctggaa tccgaccttg gaacggtact tcaagcgcaa cctcagctct atcaaacggt
     3001 tcgttgaaac gcaccagaag gaattcagga agctcctgcg gcgtgggcgc gtcgatgaga
     3061 acgaggcgca ggccgggcac aaccgggcgc ccgccgacgt cgcgtaccgc ctctacggca
     3121 tcacgaagtg ggcgaacgag aacgacgtgg accttatgat ccacgtgcac ttgaacgacg
     3181 ccggcgggcg tgcgaatgat gcgcctggcg cctacacggg ccttgcgatc tacgtcccgt
     3241 acgaggagtt cgggaatgct gaggcctcga aggcgatcgc ggacaaggtg ttcgatcacc
     3301 tcaccgatat cgccgcaaaa tccacgctcc cgatcgagga ccagggcgtg gtcgaggacc
     3361 aggaattgat cgcgcttggc tcgaacaata cgtccatagc accgagcctc cttgtcgagt
     3421 acggctacat ctacgagccg aagttcattg acgagaacgt ccgcaacatc gtcttcagcg
     3481 attacgcgta ccagaccgcc aagggcgtcc gggaattctt cggggcaagc gcgggcggcg
     3541 cccacagcac ccgggtcctg ccgtacacct ggaccgcgga catcgcgagc tcgaccgctt
     3601 caagcatccc ggcgtacgcg ctccagcacg ccttgaagga ggaggggtac tacccgccgc
     3661 gcccatcgtc tctcattaac tgcccgatcg acgggcgcat gggctcctgc gtgaccgatg
     3721 cgctgaaggc ctatcagacg gcaaagggaa tcacgccgag tggcaggctc gatgccgcaa
     3781 cgcgcaacgc cctcaatgcg aaatacagcg cagggagttt catcccagag ccgacaacga
     3841 ttgcttccgt cgcggcaccc gcagcacgct gcgctccatt cacgacgacg ctgaaattga
     3901 acagcacgga cgcggcgacc aagggccagg tttcgcgcct ccagaagata ctcgcgcagg
     3961 atacgaccat atatccagaa gggagggtga cgggcttttt cggtccggca accgataagg
     4021 cggtgaaagc cttccaggtg aagcagggca tcgcaaagcc ggggagcgct ggctacgggc
     4081 tcgtcggtcc tgcgaccggg aaggcgctca tcgcggtgtg tacgtaatac cgcacgttcg
     4141 atttacacgt cccgcatggc gtgggatgct tctcgtatat ccctaaccga cccatcatgc
     4201 gtgtacgcat ctcggtgctc ctcctctcgt tcgccttcct cttcgctcca gcatcggcgt
     4261 ccgcagcgac cgcaagcgag ctccaagccc agatacaggc gctgcttgcg cagctgagcg
     4321 tgctccaggc ccagctcaat caggcccagg gcggtacgca ttcgtcgcgc accggcgcc
//
LOCUS       DASPKG010000039         3422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_71289, whole genome shotgun sequence.
ACCESSION   DASPKG010000039 DASPKG010000000
VERSION     DASPKG010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71289"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VEA36_01415"
     CDS             complement(<1..1207)
                     /locus_tag="VEA36_01415"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HYF29007.1"
                     /translation="MVRDIVSTLRATGRRVLRPVSGLHQAAYLLAALTLASQVLALLR
                     DRLFAHQFGAGELLDLYYAAFKVPDLVFALIASLVSAYVLIPRIAGANKDETRRLLSH
                     TASFLLIAGGIICAGLALVAPSLLFALYPSFEGSAYAEEFVLLARILLIQPILLGLSG
                     IITSVTQVNRRFLLYALSPVLYNLGIIAGALFLYPTMGLVGIGWGVVLGALLHLAIHI
                     PVVAGAGLLPRPVLFSPGVVWSVARDSVPRSLALAAGAVTTLALTAIAATTGEGSIAV
                     FTLAANLEAVPLSLIGVSYATAAFPVMAEHMNAKRYEEFRTTLLTAARHIIFWSAIVS
                     VLALVLRAHIIRIVLGTGAFDWDATRLTAAILALLVLGLIAQGIILLSSRAFYAAGRS
                     WNPLLIQLGG"
     gene            complement(1245..>3422)
                     /locus_tag="VEA36_01420"
     CDS             complement(1245..>3422)
                     /locus_tag="VEA36_01420"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYF29008.1"
                     /translation="VLPPPLLLGALWFVPLAYALSTLFSGVSIPAAFFGREFEADTFG
                     FMFLLALLASLAALSFRRAKEYRAFFRTLAITLGIVLVAQVLFVIVGKVAPTLVTPTT
                     NLIGTFPDLGMVAALGVIVSLLALRLLPLPQRSRTILYVGGALALVFVALVNSPLIWT
                     MIALVALALFIEAIMRHRASSADEDLEGVALLLADSEGEVRGTDSRSLVAPLVTLAIA
                     LFFLIGGQTIGNALVTTFGTSLIDVRPSWQSTFDVGSHTYASSPLFGSGPGTFGEQWL
                     KFRDRTINETPFWNIDFISGIGSIPTSLVTTGIVGVLAWLAFLGLFLWVGIRTLLFRL
                     PQDQFMRFVAVSSFVASLVVLVFAVFTVPGPVVLALGFLFIGIFISSLRHASRANEWG
                     IVFAKSPRVGFVVVFLLTLLLLGSILAGYVVLERYLASVSYSQSALALAQGDLDKAER
                     AIERSISFAENDRAYQLASAIAIARMNQIANDTSRTQSEAQQAYQAALSAGIQAALTA
                     TKLGPNNYQNWAMLANVYQTVVPLRIEGAYDNAKTAYERAISLNPTSPVLPYVVAQLE
                     IAQGNAQAAEERLIEAINLKRDYTQAILLLSQLAVQQGRTREALQAAEAAGYFAPTDT
                     IVLFQVGILRSATGDTDGAIVALSRAVELDPQYANARFFLAVMHALKGNYSEAASQLE
                     AVAALSPENATAVAAELTALREGRNPFPPSRLGALGIPSNVTP"
BASE COUNT          672 a         1045 c         1266 g          439 t
ORIGIN      
        1 cgcccccgag ctggatcagg agcgggttcc aggagcgacc ggccgcgtag aaggcgcgcg
       61 acgacagcag gatgattccc tgggcgatga gccccagcac aagcagcgcg aggatcgcgg
      121 cggtcaaccg cgtcgcgtcc caatcgaagg cgcccgtccc gagcacgatg cggatgatgt
      181 gggcgcgaag caccaaggcg agcaccgaca cgatcgcgga ccagaagatg atgtgccgcg
      241 ccgccgtcag cagggtcgtg cggaactcct cgtagcgctt ggcgttcatg tgctcggcca
      301 tgaccgggaa ggcggcggtc gcgtaggaca cgccgatcaa ggagagcggg acggcctcga
      361 ggttcgccgc gagcgtgaac accgcgatac ttccctcacc ggtcgtcgcc gcgatcgcgg
      421 tcaaggcaag cgtggtgacc gcgcctgccg caagggcaag cgagcgcggc accgagtcgc
      481 gcgcaaccga ccataccacc ccaggcgaga agaggaccgg ccgcggcagg agtcccgcgc
      541 ccgctaccac ggggatgtgg atcgcgagat ggaggagcgc cccaaggacg acgccccacc
      601 cgatgcccac gagccccatc gtgggataga ggaagagcgc gcccgcgatg atgccgaggt
      661 tgtagaggac gggcgagagc gcgtacagga ggaaccggcg gttgacctgc gtgacgctcg
      721 taatgatgcc cgagagcccg agcaggatcg gctggatgag gaggatgcgc gcgagcagca
      781 cgaactcctc tgcatacgcg gacccctcga aggacggata gagcgcgaac agaagcgagg
      841 gcgcgacgag cgcgagtccc gcgcagatga tcccgcccgc gatgaggagg aaggacgcgg
      901 tatgcgacag gagccgccgc gtctcatcct tgttcgctcc cgcgatccgc gggatgagca
      961 cgtaggcgga gaccagggac gcgatgagcg caaagacgag gtccgggacc ttgaaggccg
     1021 cgtagtagag gtcgaggagt tcgcccgccc cgaactggtg cgcgaacagg cgatcccgca
     1081 ggagcgcgag gacctgcgac gcgagcgtca gcgccgcgag caggtacgcc gcctggtgca
     1141 gcccgctcac aggccgcagc acccggcgcc cggtcgctcg tagcgtgctg acgatgtcgc
     1201 ggaccatacc gtgccttgtg ggaactgagg agcccgtccc taggttacgg cgtgacgttc
     1261 gaggggatgc caagcgcccc gaggcgcgat ggcgggaatg ggttacggcc ctcgcgcagc
     1321 gcggtcaact cggcggcgac cgcggtcgcg ttctcgggcg agagcgccgc taccgcctcg
     1381 agctggcttg cggcctccga gtagttgccc ttcaaggcgt gcatgacggc gaggaagaag
     1441 cgcgcgttgg cgtactgcgg gtcgagctcg accgcgcggg agagcgccac gatcgcgccg
     1501 tccgtgtccc cggtggcgct ccggaggatg ccgacctgga acaagacgat ggtgtcggtc
     1561 ggggcgaagt accctgcggc ttccgcggcc tggagcgcct cgcgggtccg tccctgctgc
     1621 acggcgagct gcgagagaag gaggatggct tgggtgtagt cgcgcttgag attgatcgcc
     1681 tcgatgagcc gctcctcggc cgcttgtgcg ttcccctgcg cgatctcgag ctgcgccacg
     1741 acgtacggga gcacgggaga ggtcgggttg aggctgatcg cccgctcgta ggccgtcttc
     1801 gcgttatcgt aggcgccctc gatgcgcagc ggcaccacgg tctggtacac gttcgcgagc
     1861 atcgcccagt tctggtagtt gttgggaccg agcttggtgg cggtcaaggc tgcctggatg
     1921 ccggccgaga gcgcggcctg gtatgcctgc tgcgcctccg actgcgtgcg ggaagtgtcg
     1981 ttcgcgatct ggttcatgcg cgcgatcgcg atcgcagacg caagctggta cgcgcgatcg
     2041 ttctccgcga aggagatcga gcgctcgatc gcccgctcgg ccttatcgag gtcgccctgg
     2101 gcgagcgcga gcgcggactg gctataggag accgaggcga ggtagcgctc gagtacgacg
     2161 tagcccgcga ggatggaacc gaggagaagg agggtcagga ggaacacgac cacgaagccg
     2221 acccgggggc tcttcgcgaa tacgatgccc cactcgttcg cccggctcgc atgccggagc
     2281 gaggagatga agatgccgat gaagaggaac ccgagcgcga gcacaacggg acccggcacc
     2341 gtgaagaccg cgaagacgag caccacgagg ctcgcgacga aggacgagac cgcgacgaag
     2401 cgcatgaatt ggtcctgcgg gaggcggaac aggagggtcc ggataccgac ccacaggaag
     2461 agcccgagga aagcgagcca ggcgaggacg ccgacgatgc cggtcgtcac caaggacgtc
     2521 gggatcgagc cgatgcccga gatgaagtcg atgttccaga acggcgtctc gttgatcgtg
     2581 cgatcgcgga acttgagcca ctgctcgccg aaggtgcccg ggccagaacc gaacaagggc
     2641 gatgaggcgt aggtgtggct tcccacatcg aaggtggact gccaggacgg gcgcacatcg
     2701 atcaggctcg tgccgaaagt ggtcacgagc gcgttgccga ttgtctgccc gccgatgagg
     2761 aagaagagcg cgatcgcgag cgtcaccaag ggggcgacca acgagcgcga atcggtcccc
     2821 cgcacctcgc cctcgctgtc agcaaggaga agcgcgacgc cctcgagatc ttcgtcggcc
     2881 gaggaggcgc ggtgccgcat gatggcctcg atgaagagcg caagcgcaac gagcgcgatc
     2941 atcgtccaga tcaggggcga attcacgagc gcgacgaaca cgagcgcgag cgcgccgccc
     3001 acgtagagga tggtgcggct gcgctgcggc agcggcagga gccggagcgc gagcaatgag
     3061 acgatgacgc cgagcgccgc caccatgccg agatccggga aggtgccgat caggttcgtg
     3121 gtcggggtga cgagggtggg cgccaccttg cccacgatca cgaagagcac ctgggccacg
     3181 agcacgatgc caagcgtgat cgcaagcgtc cggaagaatg cgcggtactc cttcgcgcgg
     3241 cggaacgaga gcgcggcgag gctcgcaagg agcgcgagga ggaacatgaa cccgaaggta
     3301 tcggcctcaa attccctgcc gaagaaggcg gccgggatcg agacgcccga gaagagggtc
     3361 gagagggcgt aggccaaggg gacgaaccag agcgcgccaa gtaggagcgg cggcgggagc
     3421 ac
//
LOCUS       DASPKG010000040         6006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1424722, whole genome shotgun sequence.
ACCESSION   DASPKG010000040 DASPKG010000000
VERSION     DASPKG010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424722"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..518)
                     /gene="rpsL"
                     /locus_tag="VEA36_01425"
     CDS             complement(108..518)
                     /gene="rpsL"
                     /locus_tag="VEA36_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HYF29009.1"
                     /translation="MATINQLTKKARKPTTRKTKVVALGRGFNTLKNRPTFYPAPFKR
                     GVCTKVTTKTPRKPNSAIRKIARVRLTNGMEVTAYIPGEGHNLQEHSVVMLRGGRVKD
                     IGVQYTIVRGRLDTAGLDKRRRGRSRYGAKKPKA"
     gene            complement(625..1263)
                     /locus_tag="VEA36_01430"
     CDS             complement(625..1263)
                     /locus_tag="VEA36_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYF29010.1"
                     /translation="MKITKYGHACLLVEEGGATILLDPGVWNPVPEATGIDAVFITHE
                     HQDHIDIPQVTAVLGRNPDAVIYTHEAVGRLLEEAGLLYTPIAEGETVSIQGVPVQSF
                     GHDHAIIYGSASPCRNTGFLINERLYVPGDALHDVPLKPVEILALPTGAPWMKLAEAI
                     EYAKKVRPKVVFPIHDAMYTEEYQRGLVPRIVGRNLEGIEFRDMQAGAVEEF"
     gene            complement(1287..1571)
                     /locus_tag="VEA36_01435"
     CDS             complement(1287..1571)
                     /locus_tag="VEA36_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007255865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29011.1"
                     /translation="MAETQTAGMRRTTDHDRIERWAEERQGTPAIIESTWDGNTAVLQ
                     IDFGDPDESLMEITWDEFFRIFDDAELEFIYEEYTEDGGASRRYDITERS"
     gene            1724..1999
                     /locus_tag="VEA36_01440"
     CDS             1724..1999
                     /locus_tag="VEA36_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29012.1"
                     /translation="MRLPQTTDPEPPPVVTAETFACGTACRLYGKPLPDPPPRIGRPF
                     CGGCSVIPTNQPHQPCLSESVAQSNQAWIDQESARLGFPVALPIAAE"
     gene            complement(2142..2771)
                     /locus_tag="VEA36_01445"
     CDS             complement(2142..2771)
                     /locus_tag="VEA36_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYF29013.1"
                     /translation="MEMLVFGIIVLILSIIIHEVAHGYAAYALGDPTAKLEGRLTLNP
                     APHVDPIGSLILPALLVMTSAGILFGWAKPVPYNPYNLRNQRWGEAIVAVAGVATNLL
                     LAVLFALVARYAYASGMGDFGDLAAIVTLTNLSLGLFNLIPFPPLDGFTVLRGVLPYR
                     MSMAMREFEDRMRAGSIFTLIAFLFIFSYFFAQPFYQFISYIFGILIGA"
     gene            2816..3061
                     /locus_tag="VEA36_01450"
     CDS             2816..3061
                     /locus_tag="VEA36_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HYF29014.1"
                     /translation="MARACEVTGKSTQIGGRYSNRTRATQFNPTGKVRRSANLTKRRI
                     FVPELGTSVLVDISTAGMRTIKKNGAYATLKKAGIVK"
     gene            complement(3121..3426)
                     /locus_tag="VEA36_01455"
     CDS             complement(3121..3426)
                     /locus_tag="VEA36_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29015.1"
                     /translation="MYQTADHALSPSRLFAAELSAKLIESADPARYDRILSAFLWFVE
                     RDLRGAPPITPGHAELRYEVLVRQPLRQRNCSPEERCCVLGYLREFVLRALPVANDN"
     gene            complement(3468..4319)
                     /locus_tag="VEA36_01460"
     CDS             complement(3468..4319)
                     /locus_tag="VEA36_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HYF29016.1"
                     /translation="MAFRPRPLLLVIVIALLIALNAAIVYAFIESERRTLRVSFLDVG
                     QGDAILIESPDGVELLIDGGRDRSVLRRLPRVMGPLDRSIDMVIATHPDADHIGGLPD
                     VLSRYRVSYYLSPGIRHDTSQRERLDAAVAREGIATFTARRGQRIHLGEDVYADVLYP
                     DRDVANGETNAGSIVVRLVYGDTSFLLTGDAPEAVERYVMTLGDVDSDVLKAGHHGSR
                     TSTSESWLAAVSPETVVISAGKDNAYGHPHAEVVSRIQESGAAILSTIDEGTIIFESD
                     GGRIVRK"
     gene            4361..4810
                     /locus_tag="VEA36_01465"
     CDS             4361..4810
                     /locus_tag="VEA36_01465"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYF29017.1"
                     /translation="MFRRLSRKVIAPFQLFYWRVAMPEAEGAKVLIEHNGKFLLIRET
                     FGQKHWTLPGGRSRREEDPVATAKRKVREEVGIELPNPLPLGSYFHTRQHKRDVICAF
                     HAKVESPAYQVNPNTIAEAAWFTPEEMASLNQSESVADVLELYQKSR"
     gene            4813..5127
                     /locus_tag="VEA36_01470"
     CDS             4813..5127
                     /locus_tag="VEA36_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002108639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HYF29018.1"
                     /translation="MKRYQEEVDAWFKKQGWAYWEPLAITARLFEECGEFARLVNHVY
                     GPKRKKLDEKKQDMEEELGDIVYTLICFANSHNISLDDAIRKSMDKVTERDKDRYTPT
                     DA"
     gene            complement(5183..5449)
                     /locus_tag="VEA36_01475"
     CDS             complement(5183..5449)
                     /locus_tag="VEA36_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29019.1"
                     /translation="MKSATNVNSSFIIKIGVMAAAVLGMSVPALSFAAEYAYVNGAGE
                     VRTVTAADAMAAIRTAPGIGVHSGVLLLDSQADQDVVGDSVSGA"
     gene            complement(5745..>6006)
                     /locus_tag="VEA36_01480"
     CDS             complement(5745..>6006)
                     /locus_tag="VEA36_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HYF29020.1"
                     /translation="SYATFKKQWPGMDFIVTSPPLSYEEYPNEERSKEYLLSMMVGDL
                     QRIKEYPAKGFQIEQEIPDDVWQAYEQLVREGYTTHLIRDSV"
BASE COUNT         1188 a         1858 c         1833 g         1127 t
ORIGIN      
        1 ccgtcccaca tgacggtgtt gatgaagcgc gaaatggcct cggactgata cacgaggtcc
       61 gggcggcgcg ggtacttgtt cttaacggga tgacgcatgt tctaaagtta tgccttcggc
      121 ttcttcgcac cgtaccgcga gcggccgcga cggcgcttat cgaggcccgc ggtatcgagg
      181 cgaccgcgca cgatcgtgta ctgcacgccg atgtccttca cgcggccacc gcgcagcatc
      241 accaccgagt gctcctggag gttgtggccc tcgcccggga tgtaggcggt cacttccatg
      301 ccgttggtga ggcgcacgcg ggcgatctta cggatagccg agttcggctt acggggggtc
      361 ttggtggtca ccttggtgca gacgccgcgc ttgaagggcg ccgggtagaa ggtcgggcgg
      421 ttcttgaggg tgttgaaccc acgtccaaga gccacgacct tggtcttgcg ggtcgtgggc
      481 ttgcgcgcct tcttcgtcag ctgattgatg gtggccatgt cgtaaatgaa aactcccccg
      541 tgggagttag gcacactata tagaaaggcc tagattaatg caaacaggta cctacctccg
      601 ctttttctta aatatgtaga taacctagaa ctcctccacc gcccccgcct gcatatccct
      661 gaattcgatg ccctcgaggt tcctgcccac gattcgtggc acgagcccac gctgatattc
      721 ctccgtatac atcgcatcgt gtataggaaa cacgaccttc ggccgtacct tcttcgcgta
      781 ctcgatcgcc tcagcgagct tcatccaggg tgcccccgtc gggagcgcga ggatctcgac
      841 cggcttcaag ggtacgtcgt ggagcgcgtc gcccggaacg tagagccgct cgttgatgag
      901 aaagccggta ttccggcagg gcgatgcgct gccgtagatg atggcgtggt cgtgcccgaa
      961 gctctggacc ggaaccccct gtatggaaac ggtttccccc tccgcaatcg gcgtatacag
     1021 aagcccggcc tcctcaagca gcctacccac tgcctcatgc gtatagatga ccgcgtcagg
     1081 gttgcgtccc aagaccgcgg tcacctgcgg gatatcgata tggtcctggt gctcgtgcgt
     1141 gatgaatacc gcatcgatgc ccgtcgcctc cggcaccggg ttccagacgc ccggatcaag
     1201 caggatcgtc gcacccccct cctcgacgag gagacaggca tgcccgtact tcgttatctt
     1261 cataccgcca agtatagaac ggtcggttac gaccgctcgg tgatatcgta gcggcggctc
     1321 gcgccgccat cctccgtata ctcctcgtat atgaactcga gctccgcgtc atcgaagatg
     1381 cggaagaact cgtcccaggt gatctccatg agggattcgt cagggtcccc gaagtcgatc
     1441 tggagcaccg ccgtgttccc gtcccaggtc gattcgatga tcgcgggcgt cccctgccgc
     1501 tcctcggccc agcgctcgat ccgatcgtga tcggtagtgc gccgcattcc cgccgtctgt
     1561 gtctctgcca tacgcgcttc cattaatgta tatgcaccga agtctcgccc acgcttgctg
     1621 aaggctgcgt gagttacgca cccccagggc gcgcgacgcg tgagcgcggg tgtagactta
     1681 gcggtagcaa caagatcgtt gtgaacgcgt cggggagggc gccatgagat taccccagac
     1741 caccgatcca gaaccaccgc ccgtcgtcac tgcggagacc ttcgcctgcg gaacggcctg
     1801 ccggttgtat ggcaagccgc ttccggatcc tccgccgcgt atcggtcggc ccttctgcgg
     1861 cggttgctcg gtcatcccga ccaatcagcc gcaccaaccc tgcctgagcg agtccgtcgc
     1921 ccagagcaac caggcgtgga tcgaccaaga gagcgcacgg ctcgggttcc ccgtcgccct
     1981 gccgatcgcg gccgaatagg ctgcgcgcct ccaggaagac tgccccctaa cccggggctt
     2041 ttccttatcc cttgcgacgc ccgagtagat acccgataac tgccccgaat ccgacgagga
     2101 gcgcgcaggt caggaatcgc ttcattaccc gctagaacca gctaggcgcc gatgaggatg
     2161 ccgaagatgt aggagatgaa ctgatagaag ggctgcgcga aaaagtagga gaagatgaag
     2221 aggaaggcga tgagcgtgaa aatgctccca gcacgcatgc ggtcctcgaa ttcgcgcatc
     2281 gccatcgaca tccggtacgg caggacgccc cgcagcaccg tgaagccatc caagggcggg
     2341 aatgggatga ggttgaagag ccctaaggaa aggttggtaa gcgtgacgat cgccgcgagg
     2401 tccccgaagt cgcccatgcc gctcgcgtat gcgtagcggg cgacgagcgc gaagaggacc
     2461 gcgagcagga ggttggtcgc gacgcccgcg accgcgacga ttgcctcgcc ccaccgctgg
     2521 ttcctgagat tgtacgggtt gtacgggacg ggcttggccc acccgaagag gatccccgcg
     2581 ctcgtcatca cgaggagcgc aggcaggatg agcgagccga tggggtccac gtgcggcgca
     2641 ggattgaggg tgagcctccc ctcgagcttt gcggtcgggt ccccgagcgc ataggccgca
     2701 tagccatgcg ccacctcgtg gatgatgatg gagaggatga gcacgatgat gccgaagacg
     2761 agcatttcca tggatgttgc cgattagcgt gcccctatgc tagcatgcgg ggactatggc
     2821 acgcgcctgc gaagtaaccg gcaagagcac gcagatcggc gggcggtata gtaaccgcac
     2881 gcgtgcgacc cagttcaacc cgacgggcaa ggtccgccgg agcgcgaacc ttaccaagcg
     2941 ccgcatcttc gtccccgagc tcggcacgtc cgtcctcgtc gatatctcca ccgccggcat
     3001 gcgcaccatc aagaagaacg gcgcgtacgc caccctgaag aaagcgggga tcgtgaagta
     3061 gacgagagaa cgcccggtcc gagaaggccg ggcgttttcc ttttgggaag tctggtttcc
     3121 ctagttgtcg ttagcgacgg gcagggcgcg gagcacgaat tcccgcagat acccgagcac
     3181 gcagcagcgc tcctcgggag agcagttgcg ctggcggagc ggctgccgca cgagcacctc
     3241 ataccgcagc tcggcatggc cgggcgtgat cggcggcgct ccccgcaggt cccgttcgac
     3301 gaaccacagg aaggccgaga ggatgcggtc gtaacgagcg ggatcggcgg attcgatgag
     3361 ctttgcggag agctcggcgg cgaagaggcg actggggctg agtgcatggt cagcggtctg
     3421 gtacatacgc aaatctccct aactcctctt ggcaccttag cacggcctta cttccggaca
     3481 atacgcccgc catcgctctc gaagatgatc gtgccttcgt cgatcgtcga gaggatggcg
     3541 gcgcccgatt cctgaatgcg cgaaaccacc tccgcatgcg ggtgcccgta cgcgttatcc
     3601 ttccccgccg atatgaccac cgtctctggc gagaccgcgg caagccacga ttcgctcgtc
     3661 gacgtccgcg agccatggtg cccggccttg agcacgtcgc tgtcgacatc accgagcgtc
     3721 atcacatagc gctccaccgc ttccggcgcg tccccggtca gcaggaagga agtatctccg
     3781 tacacgagcc gcaccacgat cgatccggcg ttcgtctcgc cgtttgcgac gtcgcggtcc
     3841 gggtatagca catcggcata gacgtcctcg ccgagatgga tgcgctggcc gcgacgcgcc
     3901 gtgaaggtcg cgatgccctc gcgcgcaacc gcagcatcga gccgctcgcg ctggctcgtg
     3961 tcatggcgga tcccgggcga aagataatac gagacgcggt accgggagag gacgtccggc
     4021 agcccgccga tatggtccgc gtccggatgc gtcgcgatca ccatatctat ggagcggtcg
     4081 aggggtccca tgacccgcgg gagcctgcgg agcaccgatc ggtcgcgccc gccatcgatg
     4141 aggagctcga cgccgtccgg gctctcaata aggatcgcgt cgccctgccc cacatcgagg
     4201 aaggagacgc gcagcgtccg tcgctccgat tctatgaatg cgtacacgat cgcggcattg
     4261 agcgcgatga ggagcgcaat cacgataacg agcagcaacg gacggggtcg gaacgccata
     4321 tctggctagt atagtcggcg atacatgata gtgtttgcgt atgttccggc ggctctcacg
     4381 caaggtcatc gcccccttcc agctctttta ctggcgcgtc gccatgccgg aggccgaggg
     4441 cgctaaggtc ctcatcgagc ataacggcaa gttcctgctc atccgtgaga ccttcggcca
     4501 gaagcactgg acccttccag gcggccgcag ccgccgcgag gaggatccgg tcgcgaccgc
     4561 aaagcgcaag gtccgggagg aggtcggtat cgagctcccg aatcccctcc cgctcggctc
     4621 ctatttccat acccgccagc acaagcgcga cgtcatctgc gccttccacg cgaaggtcga
     4681 gagccccgcg taccaggtga accccaacac catcgctgag gcggcctggt tcacccccga
     4741 ggagatggcg agcctcaacc agtcggagtc ggtcgcggat gtcctcgagc tctaccagaa
     4801 gagccggtag ccatgaagcg gtaccaggag gaagtcgatg cctggttcaa gaagcagggc
     4861 tgggcgtact gggagccgct tgcgatcacc gcgcgcctct tcgaggagtg cggcgagttc
     4921 gcccgcctcg tgaatcacgt ctacggcccg aagcgaaaga agctcgacga aaagaagcaa
     4981 gatatggaag aggagctcgg cgacatcgtc tacaccctca tctgctttgc gaattcacat
     5041 aacatcagcc tcgacgacgc catcaggaag agcatggaca aggtgacgga gcgggacaag
     5101 gatcggtata cgcccacgga cgcgtagcat atacggaaca cgggacgatc agagaccgtc
     5161 ccgtgtgcgt gcggcgtggg gcttacgctc ccgagaccga atcgccgacc acgtcttggt
     5221 cagcctggct gtcgaggagg agcacgccgc tgtgcacacc gatgcccggc gccgtgcgga
     5281 tcgccgccat cgcgtcggct gcggtcacgg tgcggacctc gcccgcgcca ttgacgtatg
     5341 cgtactcagc cgcgaagctg agcgccggaa cgctcatgcc gaggacggcc gctgccatga
     5401 cgccgatctt gataataaaa ctcgagttca cgttcgttgc gcttttcata cgagagagta
     5461 cttagggtag ttacattcct tgggaatcga acccaacgaa gcaaagtgat ttcgtaagta
     5521 cacactcact aggtgcgttg gttatgcaag taggaggaat gctctggcca gcggacgacg
     5581 ggggtccggt ggcagtaccg ttgatcagat acttttgcga catagggagt atagcgtcaa
     5641 ttttgacaga atgcaagcat cattgcttac tcaattctcg tggtgctcat gcggactcta
     5701 acgtcctcca ctaggcggag ccatatcgga gcgtgttcga acttctaaac agaatctctt
     5761 attaaatggg ttgtataccc ttctctgacg agctgctcgt atgcctgcca tacgtcgtca
     5821 ggtatttcct gttctatctg aaaccccttt gccggatatt ctttgatccg ctgtaaatct
     5881 ccgaccatca tgctgaggag atattcttta cttcgctctt cattcgggta ttcctcgtat
     5941 gagagcggag gcgacgtgac gatgaaatcc atgcccggcc actgcttctt gaacgtcgca
     6001 tacgac
//
LOCUS       DASPKG010000041         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_343565, whole genome shotgun sequence.
ACCESSION   DASPKG010000041 DASPKG010000000
VERSION     DASPKG010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343565"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VEA36_01485"
     CDS             complement(<1..393)
                     /locus_tag="VEA36_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HYF29021.1"
                     /translation="MANFTLHTPFPPAGDQPEAIKKLTEGLKQGMRHQTLLGVTGSGK
                     TYTAANVIANFDKPTLVLAHNKTLAAQLAQEYREFFPDNAVHYFVSYYDFYQPEAYLP
                     VSDTYIEKEAQINEEIDRLRHASTQALLT"
     gene            complement(493..816)
                     /locus_tag="VEA36_01490"
     CDS             complement(493..816)
                     /locus_tag="VEA36_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29022.1"
                     /translation="MRSVITIAAALLMGAPAAATPVSKIPADELKAFSRHLVVRTRDG
                     AEFTAAQHEAIRARLDALQRSCQTHRSEESYFYCVAGGMSAAEVAGEFGKNVIVYSVS
                     TEPAR"
     gene            complement(899..2113)
                     /gene="tgt"
                     /locus_tag="VEA36_01495"
     CDS             complement(899..2113)
                     /gene="tgt"
                     /locus_tag="VEA36_01495"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HYF29023.1"
                     /translation="MKPVNFKVEARAGRARTGVLTTPHGVIETPAFVPVATKANVKGI
                     LPETLKELGAQVALANTYHLYLQPGEAVVRGAGGLNAFMNWNGPTMTDSGGFQVFSLG
                     AAYGRSITKIAKGEENARDAGVAVYDEGVQSQHGQLAVIDEEGVTFTSHLDGSLHRFT
                     PERSVEIQHALGADIFFAFDECTSPTADYEYQRDAMRRTHRWAERSLKAHRQNLHANK
                     KQGIFGIVQGGRYEDLREESAKEIASMDFDGYGIGGSFSKEDMMGALQAAVRHLPEEK
                     PRHFLGIGEPGDLLLGIAEGMDTFDCVAATRLGRHGTIYTMQGTINLTNEQYTNDFTP
                     LDPARIVPGTEGFTRGYVSHLLRSGEMLGQVIASMHNLGFILKLVDGAREAIQEGRFG
                     AYREAFVRSYAA"
BASE COUNT          451 a          771 c          734 g          481 t
ORIGIN      
        1 cgtgaggagc gcctgggtgg aggcgtggcg caagcgatcg atctcctcgt ttatctgcgc
       61 ttctttttcg atataggtat cagaaacagg aagatacgct tccggctggt agaagtcgta
      121 gtaggagacg aagtagtgca ccgcattgtc cgggaagaac tcgcggtact cctgggcgag
      181 ctgggcggcg agcgtcttgt tgtgcgcgag gaccagggtc ggcttatcga agttcgcaat
      241 gacgttcgcg gccgtgtagg tcttccccga cccggtaacg ccaagcaaag tctggtgtcg
      301 catgccctgc tttaggcctt cggtgagctt cttgatggcc tccggctggt cgccggctgg
      361 cgggaagggg gtgtggagcg tgaaattcgc catgacctct cagtgtatcg gtgctggctg
      421 aaatataaaa gaagagccgc cggcacccca tcgattgtat cgatggggtg ccggcggcca
      481 gcgaaggagc gattagcgcg cgggttcggt gctcaccgag tacacgatga cgttcttgcc
      541 gaattctccg gctacctcgg cggccgacat gcccccggcg acgcagtaga agtagctctc
      601 ctcgctgcgg tgggtttggc aggagcgctg gagcgcatcg aggcgcgcac gaatggcttc
      661 gtgttgagcc gcggtgaact ccgcgccgtc tctcgtccgc accaccaggt gccgagagaa
      721 tgccttcagc tcgtctgccg gaatcttaga aaccggcgtc gctgcggccg gcgcacccat
      781 caagagcgca gcggctatcg taatgacaga cctcatggat cacctcgtga ttagagaaga
      841 cgcaggggaa cctctcaagc aacgttctca accaagatgc tgctggatac tacggctgtc
      901 aagccgcata gctccgcacg aacgcctccc gatacgcgcc gaacctcccc tcctggatcg
      961 cctcccgcgc cccgtccacg agcttcagga tgaagccgag gttgtgcatg gatgcgatca
     1021 cctgcccgag catctcgccg gagcgaagga ggtgcgagac gtagccccgg gtgaacccct
     1081 cggtgcccgg gacgatgcgt gcgggatcaa gcggggtgaa gtcattcgta tactgctcgt
     1141 tcgtaagatt gatagttccc tgcatcgtat agatggtgcc gtggcgacca agccgcgtcg
     1201 ccgcgacgca atcgaaggta tccatgccct ccgcaatgcc aagcaggaga tcccccggct
     1261 ccccgatgcc gaggaaatgc cgcggcttct cctcgggaag gtgccggaca gcggcctgca
     1321 aggcgcccat catgtcctcc ttgctgaagg acccgccgat gccgtaccca tcgaagtcca
     1381 tcgaggcgat ctccttcgcg gactcctccc gcaggtcctc gtaccgtccc ccctgcacga
     1441 tcccgaagat gccctgcttc ttgttcgcat gtaaattctg ccgatgcgcc ttcagagagc
     1501 gctccgccca gcggtgcgtg cggcgcatgg cgtctcgctg gtattcgtag tccgcggtcg
     1561 gcgaggtgca ctcatcgaag gcgaagaaga tgtcggcgcc tagggcatgc tggatctcga
     1621 ccgagcgctc cggcgtgaag cgatgcagcg acccgtcgag gtgcgaggtg aaggtcacgc
     1681 cctcctcgtc gatgaccgcg agctgcccat gctgcgactg cacgccctcg tcgtagaccg
     1741 caacgcccgc atcccgcgcg ttctcctcgc ccttcgcgat cttcgtgatc gagcgaccgt
     1801 aggcggcgcc caaggagaag acttggaacc cgcccgagtc cgtcatggtg ggaccgttcc
     1861 aattcataaa tgcgttgaga ccgcccgccc ccctcaccac ggcctccccg ggctgcagat
     1921 acaggtgata ggtgttcgcg agcgccacct gcgcgccgag ctccttcaag gtttccggga
     1981 gtatgccctt gacgttcgcc ttggtcgcga ccggcacgaa ggcgggcgtc tcaatcaccc
     2041 catgcggggt cgtgagcacc ccggtacggg cgcgccctgc tctcgcttca actttgaaat
     2101 tgacgggctt catgttccct taaggtagcc gcacgtatcc tcgagagcaa gcacatatat
     2161 tctccattct gacaaggttt tctgctaaat ttgacatatc caaaatataa cggtatatac
     2221 tgcccttagg agttcttacc atctcgttag gtgagggctc tccaacgcaa cttcaaccca
     2281 cggggagttc cccatgctca agaccaagaa tctgctgggc gcgggggctg ccctgtcgct
     2341 cgtgctccag accgtggcgt tcccggctct cgccgaggcg cccgcgaaca agaccgagca
     2401 caccaccacg ttccagtacc gtggtcccgc catcctg
//
LOCUS       DASPKG010000042        17437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1968343, whole genome shotgun sequence.
ACCESSION   DASPKG010000042 DASPKG010000000
VERSION     DASPKG010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 17437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17437)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17437
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968343"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VEA36_01500"
     CDS             complement(<1..603)
                     /locus_tag="VEA36_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HYF29024.1"
                     /translation="MMSFFHTVLYTPIYNLLIFLTDIVPAGDIGIAVIVATIIVKLVI
                     LPLSLSAARTQKVLKEIEPKLKELREKYKDDKEKQAREMFALYKENHINPFAGLATLF
                     IQLPILLTLFWVFQSKTLSTVDTSLLYPFVPVPEAISPFFLGTFEIAGASILLALIAG
                     ILQFVQGKFSFTIPPKSTKTGPESIQQDFGRAMMMQMLYVL"
     gene            complement(632..946)
                     /locus_tag="VEA36_01505"
     CDS             complement(632..946)
                     /locus_tag="VEA36_01505"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HYF29025.1"
                     /translation="MLPKRLRLSRAEFGKAAAGTRATSAHFSVTVRRNAENGGAAAVI
                     SKKVAKKAVDRHLLKRRILEALRPEVFSDRSIIVYARAGAPTLSYAALKQELSELLGK
                     VG"
     gene            complement(949..1086)
                     /gene="rpmH"
                     /locus_tag="VEA36_01510"
     CDS             complement(949..1086)
                     /gene="rpmH"
                     /locus_tag="VEA36_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HYF29026.1"
                     /translation="MPKRTYQPKKKKRARTHGFRARTATPSGRKLLLRRRRKGRAKLT
                     V"
     gene            1180..2538
                     /gene="dnaA"
                     /locus_tag="VEA36_01515"
     CDS             1180..2538
                     /gene="dnaA"
                     /locus_tag="VEA36_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HYF29027.1"
                     /translation="MNTMNRLDTRELWEHMLSQVELSISQANFNTWFRDSSIVKIEDG
                     IVYIGVPSQFFRDWYLKKFHTLLLKIVRSVSYEYRNIEYLIVKDDRRKPKVEKRPTAP
                     TPELPLDEFYINKSDNLNPRYTFDTFVVGDFNDLAFSAAEAVIRRPGITYNPLFIYGD
                     TGRGKTHLIQAIGNQYKKLYPGRKVFYLTSDRFGNEYTDSLQAGTANRFKEKYRQYDL
                     LIIDDVQFFSKKDKFQEELFHLFNSLYDNNKQIIFSSDRSPAAIPDIADRLRGRFQSG
                     MTVDISEPDIVSRMAIVRRKAAVHGVTLSDEVVEHVATSISGSIRELEGVINSIVCHT
                     QVKGVPPDLAEVRQSLRSFSRPTKTVSVKHVVSKVAEFYGIDEESIYEKTRRREVVRP
                     RQVIMYILREDFSVSYPTIGSKLGGRDHTTVIHSCEKVKREVHEDAELAKEIQDIRSL
                     LV"
     gene            2561..2782
                     /locus_tag="VEA36_01520"
     CDS             2561..2782
                     /locus_tag="VEA36_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29028.1"
                     /translation="MADYGGGKGGEQGGKMKDLLTGTEIHPIFIPRRTVEFGGLFNRM
                     RGVIIEKEPVPACRNPLSPPSTPLIIVAI"
     gene            2779..3879
                     /gene="dnaN"
                     /locus_tag="VEA36_01525"
     CDS             2779..3879
                     /gene="dnaN"
                     /locus_tag="VEA36_01525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HYF29029.1"
                     /translation="MILSLDKPAFREAVGMAARFAERRSGSLPVLAGIALIAGDDGIK
                     LRATNLETGIDLRVDGTIEETGVVVLPATVLRDITSSMSGAGALTLEHAGDIVTLSQG
                     NARSTLKTLPAEDFPVLPFPDAPTSEFTLPGAVLGNLIASVVPFASTSTVRPELASVL
                     IAAEGGQLKAVATDSFRLAEKKISLSGGLPPFSMLIPARNALDIVQTLPDTDIQVSLD
                     EHQCVMKWDKGLVTTRLVAASYPDYAQIIPKAFVADAALIKKDFETALKRTAVFSDSF
                     QKIRLSFDPSKKKLRLAAQNADIGETNEEVTGGVTGDAVELSFNHRYLAAPLSIIPAE
                     TISLSASGIGRALVMRGSGDNSFLYLVMPMNQ"
     gene            complement(3911..6148)
                     /locus_tag="VEA36_01530"
     CDS             complement(3911..6148)
                     /locus_tag="VEA36_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HYF29030.1"
                     /translation="MSKKNWRTLLKKHLESPNPLVRALFIGAGVFFLVCGVAILSVAF
                     TPIPDLNSFDARKVSESTKIYDREGKTILYDLNHDMRRNVVPLSAMSKNLQLATIAIE
                     DDKFYQHSGVRLTSFIRAVLVDLREGAFVQGGSTITQQVVKNTILTGKKSPIRKVQEW
                     ILAWKLEGKYTKDEILEFYLNVTPYGGTLYGAEAASRSFFGKSASDLTLAEAAYLAAL
                     PQAPTYYSPYGNNRPALEARKERVLDRMLTLGYIDEASHASAKAEQVTFARMQGGGII
                     APHFVFYVEQYLEQKYGADVVNQGLTIITTLDTEMQNDAEEIVNRYALANEGRFDAEN
                     AALVALDPKTGQILSMVGSRNYFDPNIDGNYNIALAPRQPGSAFKPFVYATALLKGYT
                     PESILFDLPTQFSTRCSPGDVYNSEAPCYAPGNYDQTFRGPMTFTTALQQSINIPAVQ
                     TLYLAGIKNVIDLATRMGIEGLGDPKEYGLSFALGAAEVRLLDLTSAYGVFGNDGIRH
                     KPTGILEVRDRKGKVLEKFEERGELVLDTNVARQMAAIMSNNEARVPAYNPNNPLTIP
                     GYDVAAKTGTTNESRDAWTVGYTPTLAVGAWAGNNNNRPMVKEIAGYIVAPMWNEFMQ
                     KALPRRENQVFAEPAPTPSDLHPALRGQALVQTPGGTTAHSLLHWTNKDNPRGPAPAA
                     PWLDPQYQYWESPIQSWLAAGGAVAPRQPQEQPDEQELLEQLNELIEQAAELQRQSRP
                     DIGGQ"
     gene            complement(6196..6339)
                     /locus_tag="VEA36_01535"
     CDS             complement(6196..6339)
                     /locus_tag="VEA36_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29031.1"
                     /translation="MMDDEFKEEEIPEGILSEEDAVEEEEDELGDGFHEVDAVEEEEE
                     ESL"
     gene            complement(6417..6890)
                     /locus_tag="VEA36_01540"
     CDS             complement(6417..6890)
                     /locus_tag="VEA36_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HYF29032.1"
                     /translation="MRVLGIDPGFDRLGLAVIEGDPSRPTHIWSDCVIPAKGTADERL
                     AVVHAAVVRAIEAHRPDELAIETLYFSTNKSSALGVAEARGAVLSAAGAHSIPVTEHA
                     PGSVKLSVTGSGRADKRAIAQMLPKLIALPAKKRLDDELDAIAVAICALSTRKRS"
     gene            complement(6871..7428)
                     /locus_tag="VEA36_01545"
     CDS             complement(6871..7428)
                     /locus_tag="VEA36_01545"
                     /inference="COORDINATES: protein motif:HMM:NF045213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYF29033.1"
                     /translation="MAGHSKWAQIKRQKAVTDAAKSRTFSRFARLITIEAKKAGGNLS
                     SPSLASAIERAKAVNMPKDNIERAIQKGASKDAAALEQVVYEFYGPGGTALIVDALTD
                     NKNRTTQEIKHLLSKNGFELGGQGSALWAFSKSGDGTLAPNEPLADLSEADEEALGRL
                     LELFDEHEDVQQVYTNARGYESTRD"
     gene            complement(7474..7719)
                     /locus_tag="VEA36_01550"
     CDS             complement(7474..7719)
                     /locus_tag="VEA36_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29034.1"
                     /translation="MRGPDLDRERSERRLSLTEFMHSYNEGLPPEYPRVSVSILETFI
                     GIYPELFKRKEAWSLDQHRKRVMDWLPSYIRYSTQEK"
     gene            complement(7875..8075)
                     /locus_tag="VEA36_01555"
     CDS             complement(7875..8075)
                     /locus_tag="VEA36_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HYF29035.1"
                     /translation="MQQGTIAGLTDKGFGFIKIEGAAKDLFFHSNELVDVQFDSLQVG
                     DTVSFEIGEGPKGAHATNVRKI"
     gene            complement(8206..9186)
                     /gene="ruvB"
                     /locus_tag="VEA36_01560"
     CDS             complement(8206..9186)
                     /gene="ruvB"
                     /locus_tag="VEA36_01560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HYF29036.1"
                     /translation="MSSGSDTIRLKDQSLDAALRPTLWDEYVGQGHVKANVQVVLTAA
                     KKRGDMPEHLLFYGPPGVGKTTLAHLVAATLEAPLKSTSGVAIERAGDLAAILTNLEP
                     GTVLFIDEIHQLSHKIEELLYPALESGSLDIVLGKGPSARTVELALPPFTLVGATTRV
                     AQLSSPLRSRFGGGVYQLDFYNDDDLRRIIRRSAGVLDLSVDDAVVERIASRSRATPR
                     VANALLKRVRDYSEVEDKAITPELCDEACELFGIDSYGLTKDDRRYLETLRDGFKGGP
                     AGIRALASAMHEDPDTVENVYEPYLLRLGLIERSPRGRVLTSKGREYLER"
     gene            complement(9456..11126)
                     /locus_tag="VEA36_01565"
     CDS             complement(9456..11126)
                     /locus_tag="VEA36_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HYF29037.1"
                     /translation="MAAHFDTDAEDAKLAAVREKEEEDLVRILSEKHGIPYADLSGVP
                     VNMDALRVVPEEVAKEAEAIAFDKSGKHISLAIHAPENLALGKLVGELEGQGLIVEKY
                     LVSKKSLEKGLSRYIDLSYAHEDHAGVFDIRGEELHAFAEKLTTLASLRAQLTESTDP
                     AHRAQVSIVLEQLLAAGLSLRASDIHIEPGEDGVRLRLRIDGLLTDAFTFDQHEYELI
                     ASRIKLLSGLKLNVKDRPQDGRFSIHVNNDIEVRTSVIPGAYGESFVMRLLNPNTLSI
                     SFEGLGIHEKILERLSDEIKRPNGMLLTTGPTGSGKTTTLYAFLKYLHTPSIKIITIE
                     DPVEYHIPGIVQTQVDHKDYTFAGGLRSVLRQDPDVIMVGEIRDAEVAETAIQAALTG
                     HFVFSTLHTNNAAGTFPRLVDLGVDPKSFASAITVAMAQRLVRKLDPERRKQVVLTDD
                     EKRAIERIFSTVEDRSLLPSSIETAWVPDPRSEDETGYAGRMGLFEVIFMDDELGEFL
                     RDNPSEHDIQKHVRRQGTLTMAQQGVLDALNGLTSLEEVLRVVDLPRD"
     gene            11223..11750
                     /locus_tag="VEA36_01570"
     CDS             11223..11750
                     /locus_tag="VEA36_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29038.1"
                     /translation="MFRVLTVAAVLCGSGLIASPAVAQCGSPYKVIDNSPLGPYGGYT
                     NSVPNYATNREVVTNQQYEECMQRWRSQQLYLQTQEGADGGTRRRAPATEKPLVQYVR
                     DHETGEVVAQESTMPLPDRRDGRRPTKGAQWSCPWDPVAKVWIRDQCDWKYTQKHRAR
                     DRTYYGWRPYFGYSH"
     gene            11817..12251
                     /gene="tsaE"
                     /locus_tag="VEA36_01575"
     CDS             11817..12251
                     /gene="tsaE"
                     /locus_tag="VEA36_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HYF29039.1"
                     /translation="MRRLATLEELHAEATALAARLTPRSDRATLVTLSGELGAGKTSL
                     TQALARAYGIEEHVTSPTFVLEKIYRLPEGKPFAHLVHIDAYRLKGGAELAPLGFDEL
                     MKDSGTLVVLEWPELVADGLPVADVSIQLSVQPDETRLMTYG"
     gene            12244..14619
                     /locus_tag="VEA36_01580"
     CDS             12244..14619
                     /locus_tag="VEA36_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HYF29040.1"
                     /translation="MAKAKKEKKIRLVLLDAHAIIHRAYHALPEFTGPDGQPTGALYG
                     LSSMLLRIMGELKPDYVLAAYDLAGPTVRHEAYEDYKGTRQKTDDALIAQLDTSRRVF
                     EAFSVPIYEREGFEADDILGTIVKLEKKNQDIEIIIASGDMDTMQLIDGKRVQVYTLK
                     KGLNDTILYDEEAVKERYGFPPELIPDYKGLRGDPSDNIKGVPGIGEKLATELVQKFG
                     SMEDLYKKLKKDEAALLAAGIKPRIVNLLKEHEEDAFFSKMIATIRTDAPIAYRLPEK
                     DWNECITLPKVLALFDELGFRSLRPRAVSLFGEQGAEYEELPAEESVASDRLAQAKVM
                     LWLLHSDFTNPTLDDVLAYTKAKNFDEAYATLEKNLAETGRLKQVYDEIERPLIDIVR
                     EMGECGVKIDTAVMESLRKKYRAELEGIEQGIYAMVGHEFNIASPKQLGDVLFDELQL
                     VVKGQKKTAGGQRSTKESELEKIRDLHPIVDEVLRYRSLGKLLGTYIEAIPPLLDSES
                     RLHAEFLQTGAVTGRMASQNPGLQNIPIKTPEGRAIRNAFIARDGSVLVALDYSQIEL
                     RIAAILSKDEKLIEIFKSGRDVHREVAAAVFNVEPELVDAEMRRRAKVINFGILYGMG
                     VNALKAQLGVPLSEAHAFHDDYFQTFDTLAKYLESTRGFARKHGYTETLFGRRRQFPE
                     MKSPLPYVRAQAERMAINAPIQGTQADIIKKAMVEVDRMLHERGKKDDARLILQVHDE
                     LVYEIREEDAKALAADIKQIMEEVLPGQEANGVPIIAEGKLGKNWGEMSPL"
     gene            14677..14835
                     /locus_tag="VEA36_01585"
     CDS             14677..14835
                     /locus_tag="VEA36_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29041.1"
                     /translation="MSELAFNTSIVPLTEDTAPRGSRPRGWAIAAVLSLLIWFSPYII
                     WSAIHALT"
     gene            14905..15552
                     /locus_tag="VEA36_01590"
     CDS             14905..15552
                     /locus_tag="VEA36_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29042.1"
                     /translation="MIYLFHGSDVHKVRAKAFQWVAAARAKAPDAAYLRLTADQLTRE
                     SLLDALASQGLFFAKTLVLLDDPFSEREAGELVLENLTALKDSPNPVAVLAPRLIASR
                     AMKLEAAAEKAFKIDAVAKKERGFNSALVNALAARDRLLLWKEIVKAMRDGEAPEAIH
                     GLLHWKARDLMQKGGRAWTPTEARQLSVSLIELLSDSRGRDLPLPLALERFALGL"
     gene            15562..15978
                     /locus_tag="VEA36_01595"
     CDS             15562..15978
                     /locus_tag="VEA36_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29043.1"
                     /translation="MHKTLIAVVILTLVGAGLYYVLTQYQPNPESASDRQMPGTGSLF
                     STDGEVTGTIVLINTEHAAVDGPYVITVRQAGGESAIIAVPSMGLPTCAAREKIADVY
                     ALQPGQVVSARGTVGEGGIIIPCESSEHFLSLVESN"
     gene            complement(15983..16055)
                     /locus_tag="VEA36_01600"
     tRNA            complement(15983..16055)
                     /locus_tag="VEA36_01600"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16020..16022),aa:Lys,seq:ttt)
     gene            complement(16074..16967)
                     /locus_tag="VEA36_01605"
     CDS             complement(16074..16967)
                     /locus_tag="VEA36_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29044.1"
                     /translation="MSDGILFLAIFLFFFLLWFVSGGPTRPISFAGPYITPITDVDDT
                     QFAYDSDEPFFSPDFNLFGGTNLQAGDAYANASPLRGQVTISSVTPGSSAKDEYVTLQ
                     LSQNAPGPVSISGWRLRSDATYKSATIPGGTELPRRGINRTDPIVLQPGDRATITTGD
                     SPIGVSFRENRCVGYFGTSQNFSPHLSSACPSPLAEFERYYEGNKLADDSCYNYLSAL
                     PQCTNPPHRGRPRLTNRCEEFIDEYLDYNGCVAAHQGEQDFRSQEWRIYLERNAKLWK
                     PSREAVRLIDAQGMTVDLYTY"
     gene            complement(16980..>17437)
                     /locus_tag="VEA36_01610"
     CDS             complement(16980..>17437)
                     /locus_tag="VEA36_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HYF29045.1"
                     /translation="ERIEEGQGFQAVVDYAHTPDSLAALYSAYPGRKICVLGNTGGGR
                     DTWKRPEMGRIADEACDEVILTNEDPYDEDPRAIVEAMAAGMKRAPKIIMDRREAIGA
                     ALSLARPGDAVLITGKGTDPYIMEANGKKTPWSDAAVVREELKKLAGGA"
BASE COUNT         3249 a         5343 c         5578 g         3267 t
ORIGIN      
        1 caacacgtag agcatctgca tcatcatggc gcggccgaag tcctgctgga tggattcggg
       61 gccggtcttg gtgctcttcg gcgggatggt gaaagagaac ttcccctgca cgaactggag
      121 gatgccagcg atgagtgcga gcaggatgct tgcgcccgca atctcaaacg tgccgagaaa
      181 gaagggggag atggcctcgg ggaccggcac gaaggggtag aggagggagg tgtccaccgt
      241 cgagagggtc ttcgactgga acacccagaa gagggtgagg aggatgggga gctggatgaa
      301 gagggtggcg agcccggcga atgggttgat gtggttctcc ttatagagcg cgaacatctc
      361 acgggcctgc ttctccttat catccttgta tttctcccgg agctccttca acttcggctc
      421 gatctccttc aataccttct gggtacgggc ggccgagagc gagagcggaa ggatgacgag
      481 cttcacgatg atggtcgcga cgatgaccgc gatcccgatg tccccggcgg ggacgatatc
      541 cgtgaggaag atgaggaggt tgtagatcgg ggtgtagagg accgtatgga agaaagacat
      601 catgggcctc acgataccgt atccggcctc cttagcctac ctttcccagg agctccgaga
      661 gttcttgctt gagcgcggcg taggagaggg taggggcgcc cgcgcgggca tagacgatga
      721 ttgaacggtc cgagaagacc tccggccgga gcgcctcgag gatccggcgc ttcaggaggt
      781 gccggtctac ggctttcttg gccaccttct tcgagatgac ggccgcggcg cccccgttct
      841 cggcgttcct gcggaccgta acggaaaaat gcgcgcttgt ggcgcgggtt ccggcagctg
      901 ccttcccgaa ctccgcccgt gaaagccgga gtctcttcgg gagcatggct agaccgtgag
      961 cttggcgcgg cccttgcggc ggcggcgaag caggagcttg cgccccgagg gcgtcgctgt
     1021 gcgggcgcgg aagccgtgcg tgcgggcgcg cttcttcttc ttcggctgat acgttcgctt
     1081 tggcataatc cacaggatag taacaggttg acgctggatg agcaagaaaa ccggcgcttt
     1141 cgcactttat ccacagtacg ggtatacttg gcttcaccta tgaatacgat gaatcgtctt
     1201 gatacgcgtg agctctggga acacatgctc tcgcaagtcg aactatccat ctcgcaagca
     1261 aacttcaaca cctggttccg cgacagctcc atcgtcaaga tcgaggacgg catcgtctat
     1321 atcggcgtcc caagccagtt ctttcgcgac tggtacctca agaaattcca tacactcctc
     1381 ctgaagatcg tgcggtcggt gtcctacgag taccggaaca tcgagtacct catcgtgaag
     1441 gacgaccgcc ggaaaccgaa ggtcgagaag cgcccgacgg cgcccacgcc cgagctgccc
     1501 ctcgatgagt tttacattaa taagagcgat aacctcaacc cccgctacac cttcgatacc
     1561 ttcgtggtgg gggatttcaa cgatctggcc ttctcggccg cggaagccgt catccggcgt
     1621 cccggcatca cctacaatcc gctcttcatc tatggggaca ccgggagagg gaagacccac
     1681 ctcatccagg ccatcgggaa ccagtacaag aagctctatc ccggccggaa ggtcttctac
     1741 ctgacctcgg accgcttcgg gaacgagtac acggactcgc tgcaggccgg gaccgccaat
     1801 cgcttcaagg agaaataccg ccaatacgac ctgttaatca ttgacgatgt gcagttcttc
     1861 tcgaagaagg acaagttcca ggaggagctc ttccacctct tcaattccct ctacgacaac
     1921 aacaagcaga tcatcttctc atcagaccgg tcgcccgccg cgatcccgga tatcgcggac
     1981 cgcctccgag ggcgcttcca gagcggcatg acggtggaca tctcggagcc ggacattgtt
     2041 tcgcgcatgg ccatcgtgcg ccgcaaggcc gcggtgcatg gcgtcacgct ctccgacgag
     2101 gtcgtggagc acgtcgcgac ctccatctcc ggctccatcc gcgagctcga gggcgtcatc
     2161 aactccatcg tctgccacac gcaggtgaag ggggtcccgc cggacctcgc ggaggtccgc
     2221 cagtcgctgc gctccttctc ccggcccacg aagaccgtct ccgtgaagca tgtggtatcg
     2281 aaggtcgcgg agttctacgg catcgacgag gagagcatct acgagaagac ccgcaggcgg
     2341 gaggtggtgc gtccccgcca ggtcatcatg tacatcctgc gcgaggattt cagcgtctcg
     2401 taccccacga tcggctcgaa attgggcggc cgcgaccaca cgaccgtcat ccactcctgc
     2461 gaaaaagtga agcgtgaggt ccatgaggac gcggagctcg ccaaggagat ccaggatatt
     2521 cggagcctct tggtataact ccggggacaa ccctgttgat atggcggact acggaggagg
     2581 aaaaggtggg gaacagggag ggaaaatgaa ggatttatta acaggcactg aaattcatcc
     2641 cattttcatc cccaggagaa cggtcgagtt cggtgggctt ttcaacagga tgagaggggt
     2701 gataatagag aaagagcccg tgcctgcctg ccgaaatccc ttatccccgc catcaacacc
     2761 cctaataata gtagcgatat gattctttct ttagataaac ctgcattcag ggaggcggtg
     2821 gggatggcag cgaggttcgc agagcgaagg agtggtagtc ttccggtgct cgccggcatc
     2881 gccctcatcg ccggcgacga cggcatcaag ctgcgcgcga cgaacctcga gaccggaatc
     2941 gatctccggg tggacgggac gatcgaggag accggcgtgg tcgtgctccc cgccaccgtg
     3001 ctccgcgaca tcacctcctc tatgtcgggc gcaggcgccc tcaccctcga gcacgcggga
     3061 gatatcgtca ccctttctca aggaaacgcc cgtagcacgc tcaagaccct acctgccgag
     3121 gatttccccg tgctcccgtt cccggacgcg cctacgagcg aatttacgct tcctggggcg
     3181 gtcctcggca acctcatcgc ctccgttgtt ccgttcgcat cgacctccac cgtgcgtccc
     3241 gagcttgcga gcgtcctcat cgccgccgag ggcgggcaac tgaaggcggt cgcaaccgac
     3301 tccttccggc ttgccgagaa gaagatctcg ctttcgggcg gcctcccacc cttttctatg
     3361 ctcattcctg cgaggaacgc gctcgatatc gtacagacac tgccggatac tgatatccag
     3421 gtgtccctgg acgagcatca gtgcgtcatg aagtgggaca aagggcttgt gacgacccgc
     3481 ctggtcgctg cgagctaccc cgactacgca cagatcatcc ccaaggcttt cgtcgcggat
     3541 gccgcgctca tcaagaagga cttcgagacg gccctgaagc gcaccgccgt cttctcggac
     3601 tccttccaga agatccggct ctccttcgat ccaagcaaga agaagctccg gcttgcggcc
     3661 cagaatgcgg atatcgggga gacgaacgag gaggtcacgg gcggggtgac gggagacgcc
     3721 gtcgagctct ccttcaacca ccgatacctc gcggcgccgc tttccatcat tcccgcagag
     3781 accatctcac tctccgcatc agggatcggg cgggcgctcg tcatgcgggg gagtggcgat
     3841 aactcattcc tatacctcgt gatgccgatg aaccaataag aaaagacctc gggatgcccg
     3901 aggtcttttc ttattgtcca ccgatatccg gacgggactg ccgctggagt tccgcggcct
     3961 gctcaataag ttcattgagc tgctcgagga gctcctgctc atcaggctgt tcctgcggct
     4021 ggcggggcgc caccgcgccg ccagcagcaa gccaggactg gatgggggac tcccagtatt
     4081 ggtactgcgg atcgagccaa ggggcggcag gcgcagggcc acgaggattg tccttgttgg
     4141 tccaatggag gaggctgtgc gcggtggtgc cgcctggcgt ctggacgagc gcctggccac
     4201 gaagcgcagg atggagatcc gacggggtgg gagcgggctc cgcgaagacc tggttctcgc
     4261 ggcgcgggag cgctttctgc atgaactcgt tccacatggg cgcgacgata tagcccgcga
     4321 tctccttcac catagggcgg ttattgttgt tgccggccca ggcgcctacc gcaagtgtgg
     4381 gggtgtagcc gaccgtccac gcgtcgcggg actcgttcgt ggtgccggtc ttcgcggcga
     4441 cgtcgtagcc tgggatggtg agcgggttgt tcgggttata cgctgggacg cgcgcctcgt
     4501 tgttggacat gatggccgcc atctggcgcg ccacgttcgt atcgagcacg agctcgccgc
     4561 gctcctcgaa cttctcgagg accttgccct tcctatcgcg cacttcgagg ataccggtcg
     4621 gcttgtggcg gataccgtcg ttgccgaaga cgccgtaggc gctcgtaagg tcgaggaggc
     4681 gcacttccgc ggcgccgagc gcgaacgaga ggccgtactc cttcggatcg ccgagccctt
     4741 cgatgcccat gcgggtcgca agatcgatca cgttcttgat gcccgcgagg tagagcgtct
     4801 ggaccgccgg gatgttgatc gactgctgga gcgcggtcgt gaaggtcatg ggcccccgga
     4861 aggtctgatc gtagttaccg ggcgcgtagc agggcgcctc gctgttatag acgtcgcccg
     4921 gggagcacct cgtcgagaac tgggtcggaa ggtcaaagag gatggactcc ggggtgtagc
     4981 ccttgaggag cgcggtcgcg tacacgaagg gcttgaaggc cgaaccgggc tgccggggcg
     5041 cgagcgcgat gttgtagttg ccgtcgatgt tcgggtcgaa gtagttgcgg gaccccacca
     5101 tcgagaggat ctggccggtc ttggggtcta gcgcgacgag ggcagcgttt tccgcatcga
     5161 agcggccttc gttggcgagc gcgtagcggt tcacgatctc ctcggcatcg ttctgcatct
     5221 cagtgtcgag ggtcgtgatg atcgtgaggc cctggttcac gacgtccgct ccgtacttct
     5281 gctcgaggta ctgctcgaca tagaagacga agtgcggcgc gatgatcccg ccgccctgca
     5341 tgcgcgcgaa ggtcacctgc tctgccttcg cggatgcgtg gcttgcctca tcgatatacc
     5401 cgagcgtgag catgcggtcg aggacgcgct cctttctcgc ttcgagggcc gggcggttgt
     5461 tgccgtaggg ggagtaatag gtcggggcct gggggagcgc ggcgaggtac gccgcttccg
     5521 cgagggtgag atcgcttgcg gacttcccga agaaggagcg gctcgcggcc tcggcgccat
     5581 agagggtgcc gccgtaggga gtaacgttga ggtagaactc gaggatctcg tccttcgtgt
     5641 actttccctc gagcttccag gcgaggatcc actcctgcac cttgcggatg gggctcttct
     5701 tgccggtgag gatggtgttc ttcaccacct gctgggtgat ggtcgagccg ccctggacga
     5761 aggcgccctc ccggaggtcg acgaggaccg cacggatgaa ggaggtgagg cggacgcccg
     5821 agtgttggta gaacttatcg tcctcgatcg cgatcgtcgc gagctggagg ttcttggaca
     5881 tcgcggatag ggggaccacg ttgcggcgca tgtcgtggtt gaggtcgtag aggattgtct
     5941 tcccctcgcg gtcataaatc ttcgtagatt cagagacctt ccgggcatcg aaggaattga
     6001 ggtccgggat gggggtgaag gcaaccgaga ggatggccac cccgcagacg aggaagaata
     6061 cccctgcacc gatgaataag gcccgtacga ggggattcgg gctctcgaga tgcttcttca
     6121 gcagcgtgcg ccagttcttt ttgctcatgc gggccatgct aacacaaacc gcggcccttt
     6181 tgggccgcgg ctctgctaga ggctctcctc ttcctcctcc tcgacagcat ccacctcgtg
     6241 gaagccgtcg ccgagctcat cctcttcctc ctctaccgcg tcttcctcgc tgaggatgcc
     6301 ctcgggaatc tcctcctcct tgaattcatc gtccatcata taacaacgtt tatatggtta
     6361 tcctcgcagg ccgcgaagcc cctctatttt ggagagacgc ggggaagttt gcaaccttag
     6421 ctgcgcttgc gtgtggacaa ggcacagatg gcgaccgcga tggcgtcgag ctcatcgtca
     6481 aggcgcttct tggcaggcag cgcgatgagc ttcgggagca tctgggcgat ggccctcttg
     6541 tccgcccgcc cgctcccggt cacggagagc ttcaccgaac caggggcgtg ctcggtgacg
     6601 gggatgctat gggctccggc tgccgagagg accgcgccgc gggcttcggc gacgcccaag
     6661 gccgatgact tattcgtact gaaatacagc gtctcgatgg cgagctcgtc cggccgatgc
     6721 gcttcaatgg cgcggacgac cgccgcatgc accacagcga gccgttcgtc ggccgttcct
     6781 ttcgccggga tgacgcagtc gctccagata tgggtcgggc gcgacggatc gccctcgatg
     6841 accgcaaggc cgagccgatc gaagcccgga tcaatcccga gtactctcat accctcgcgc
     6901 gttcgtgtac acctgctgca catcctcgtg ctcgtcgaag agctcaagga gcctgccgag
     6961 cgcttcctcg tccgcttccg agaggtccgc caagggctcg ttcggggcga gcgtgccgtc
     7021 cccggacttg ctgaaggccc agagcgcgga gccctggccg ccgagttcga acccattctt
     7081 cgagaggagg tgcttgatct cctgggtagt gcggttcttg ttgtcggtga gggcatcgac
     7141 gatgagcgcg gtgccgcccg ggccatagaa ctcgtagacg acctgctcga gcgcggccgc
     7201 gtccttggag gcgcctttct gtatggcccg ctcgatattg tccttgggca tattgacggc
     7261 cttcgcccgc tcgatggccg aggcaaggct cggggaggag agattcccgc cggccttctt
     7321 cgcctcgatg gtgatgaggc gggcgaatcg ggagaaggta cggctcttgg ccgcgtccgt
     7381 caccgccttc tgccgcttga tctgcgccca tttgctgtgt cctgccatgc gtccactcta
     7441 ccaaaaagga gggaagtggg gcacagcatc cgctcatttc tcctgcgtgc tgtagcggat
     7501 ataggagggc agccagtcca ttacgcgctt gcggtgctga tcgagggacc aggcctcctt
     7561 gcgcttaaaa agctcgggat agatcccgat gaacgtctcg aggatggata cggacacgcg
     7621 cgggtattcc ggcggaaggc cttcgttgta cgaatgcatg aactccgtca gggagagcct
     7681 gcgttcggag cgttcgcgat cgaggtcggg tccgcgcata aataattacc gcgccgcctg
     7741 ccgcgcgtgc ttgatcgcga gacgcttctt ggtcttaggc ggatgcttgc ggattttctt
     7801 ggggctcact ccctgctttc ggttcgacat atggttgtgg tatgacgcga gcgcccgtgg
     7861 gcgctctgcg ttggttaaat cttgcggacg ttggtcgcgt gagcgccctt cgggccctca
     7921 ccgatctcaa acgacaccgt gtcaccgacc tggagggaat cgaactggac atcaacgagc
     7981 tcgttggagt ggaagaacaa gtccttcgct gctccctcga tcttgatgaa tccgaatccc
     8041 ttatccgtaa ggccggcaat agttccttgc tgcatgacgt tggtagacaa atacacctga
     8101 taactatcaa gtgcaggagg ctgaaaacga ggagtcgacg tgctacgagt gcccagcgct
     8161 gcgatttgac ctgatgacta tagcattaac cgcgcaaaaa agcaactatc gctcgagata
     8221 ctccctccct ttgctcgtca gtacgcgccc gcgcggcgat cgttcgatga ggccaagccg
     8281 gagcaaatat ggctcataca cgttctcgac ggtgtccggg tcctcatgca tggcgcttgc
     8341 aagcgcccgg atgccggctg ggccgccctt gaacccatcc cggagcgtct cgaggtaccg
     8401 acggtcgtcc ttggtgaggc catagctatc gataccgaag agctcgcagg cctcgtcgca
     8461 gagctcgggg gtgatggcct tatcctcgac ctcggagtag tcgcgaacgc gcttgaggag
     8521 cgcgttggcg acgcgcgggg tcgctctact tcggctggcg atgcgctcca cgaccgcgtc
     8581 atcgaccgat agatcgagca cgcccgccga gcgccggatg atgcgccgca gatcgtcgtc
     8641 gttatagaaa tcgagctggt agacgccgcc cccaaagcgc gaccgcagcg gggaagagag
     8701 ctgcgcgacg cgggtcgtgg cgcctacgag cgtgaatggg ggaagcgcga gctctacggt
     8761 cctcgcggaa gggcccttcc cgaggacgat atccagggac ccggactcga gcgcggggta
     8821 gagcagctcc tcgatcttat gcgagagctg gtggatctcg tcgatgaaga ggaccgtgcc
     8881 cggttcgagg ttcgtgagga tggcggcgag gtccccggcc cgctcgatgg cgaccccgga
     8941 ggtgctcttc agtggcgcct cgagcgttgc cgcaacgagg tgcgcaaggg tggtcttccc
     9001 gactcctggc gggccgtaga acaggaggtg ctcgggcata tccccgcgct tcttggccgc
     9061 ggtaagcacc acctggacgt tggctttcac gtgcccctgg cctacatact cgtcccagag
     9121 ggtgggacgg agtgccgcgt ccaaggactg gtctttcagg cgaatcgtgt cagaaccact
     9181 tgacatacaa agagggtatg ctagtattct agcacacagg gagttggcct tgtgagaacc
     9241 ttctcaccgg gcatttttct tttcggatcc tacccatcac cctctaggca aggcttgacg
     9301 gccctcgggg cgacttccca aggacctcct ggtactgcgc ttgacgcaga gcgctgggag
     9361 catcctcagg catttcgttc tgcctggagg gtaatgtgcg ggatccatgg agaacgcacg
     9421 ttcgcaggcg cgccttacgg ggcgcgtctt gcgcgttagt cccgcggcag atcaaccacc
     9481 cgcagcacct cctcgaggga ggtaagaccg ttgagcgcat cgaggacgcc ctgctgcgcc
     9541 atggtaaggg tgccctggcg gcgcacgtgc ttctggatgt cgtgctcgct cggattgtcg
     9601 cgcaggaact cgccgagctc gtcgtccatg aagatgacct cgaagagtcc catgcggccc
     9661 gcgtacccag tctcgtcctc gcttcgggga tccggcaccc acgcagtttc aatagaggag
     9721 ggaagaaggg accggtcctc gacggtcgag aagatccgct cgatggcccg cttctcatca
     9781 tcggtaagca cgacctgctt cctgcgctcg ggatcgagct tgcggacgag gcgctgcgcc
     9841 atcgcgaccg tgatcgccga cgcgaacgac ttgggatcca ccccgaggtc gacgaggcgg
     9901 gggaaggtgc cggctgcatt gttggtgtgg agcgtggaga agacgaagtg gcccgtgagc
     9961 gccgcctgga tcgcggtctc cgcgacctcg gcatcgcgga tctcgccgac catgatcacg
    10021 tccgggtcct ggcggagcac ggaacggagt cctccggcga aggtgtagtc cttatggtcc
    10081 acctgcgtct gcacgatgcc cgggatgtgg tactcgaccg gatcctcgat cgtgatgatc
    10141 ttgatggagg gcgtatggag atacttgagg aaggcgtaga gggtcgtcgt cttgccggag
    10201 ccagtggggc cggtagtgag gagcatgccg ttcggccgct tgatctcgtc cgagagccgc
    10261 tcgaggatct tctcgtggat gccgaggccc tcgaaggaga tggagagggt attcgggttg
    10321 agcaggcgca tcacgaagga ttcgccatag gcgcccggga tgaccgaggt gcgcacctcg
    10381 atgtcgttgt tcacgtggat ggagaagcgg ccgtcctgcg gacgatcctt cacattcaac
    10441 ttcaggcccg ataggagctt gatccgggaa gcgatgagct cgtactcatg ctgatcgaag
    10501 gtgaaggcgt cggtcagcag cccgtcgatg cgaagccgga gccgcacgcc gtcctccccc
    10561 ggctcgatgt ggatgtcgga tgcccggagc gagaggcctg ccgcgagcag ttgctcaagc
    10621 acgatcgaga cctgcgcacg atgcgccggg tccgtgcttt cggtcagctg cgcgcgcaag
    10681 gacgcgaggg tcgtgagctt ctccgcaaac gcgtggagtt cctcgccgcg gatgtcgaag
    10741 acgcccgcat ggtcctcgtg cgcgtaggag aggtcgatat agcgggagag gcccttctcg
    10801 aggctcttct tcgagaccag atacttctcg acgatgagcc cctggccttc gagctctccg
    10861 acgagctttc cgagcgcgag gttctcgggc gcgtggatgg ccaaggagat atgcttgccg
    10921 ctcttatcga aggcgatggc ctccgcttcc ttcgccacct cctccggaac gacccggagc
    10981 gcgtccatgt tcaccgggac gccggagagg tccgcgtacg ggatgccgtg cttctccgag
    11041 aggatgcgga cgaggtcctc ctcctccttc tcgcgcacgg ccgcgagctt cgcgtcctcg
    11101 gcgtccgtat cgaaatgagc ggccatatac gccacatcct agcacctcct taggtgagtg
    11161 cagggattga caatacacca atattctgta tactagtaac aaacatccac ggaggacctc
    11221 ccatgtttcg cgttctcacg gtggctgcgg ttctttgcgg atccgggctc atcgcttcgc
    11281 cggcggtcgc gcagtgcggc tcgccctaca aggtcatcga caacagcccg ctcgggccgt
    11341 acggtggcta caccaacagc gtgccgaact acgcgaccaa ccgcgaggtg gtcacgaacc
    11401 agcagtacga ggagtgcatg cagcggtggc gcagccagca gctctacctg cagacgcagg
    11461 agggcgctga cggcggcacc cgccgacgag cgcccgcgac cgagaagccg ctggtccagt
    11521 acgtccgcga tcacgagacc ggcgaagtgg tggcacaaga gtccaccatg ccgctcccgg
    11581 accgaagaga cggccgtcgg cccacgaaag gagcccaatg gtcctgcccc tgggatccgg
    11641 tggcgaaggt ctggatacgg gaccagtgcg actggaagta cacccagaag caccgggccc
    11701 gcgaccggac ctactatggc tggcgcccct acttcggcta cagccactag ctgcgccgag
    11761 cctgatcacg cgaggcccgc cccacaaagg cgggcctttt cgtatacaat ccctctatgc
    11821 gccggctcgc caccctcgaa gaactccacg ccgaagcgac agcccttgct gcccgcctta
    11881 cccctcgttc agatcgcgcc actctcgtta ccctctccgg ggagctcggg gcagggaaga
    11941 cctcccttac ccaggcgctc gcacgggcgt atggcatcga ggagcacgtg acgagcccca
    12001 ccttcgtgct cgagaagatc taccggcttc cagaggggaa gcccttcgca cacctcgtgc
    12061 acatcgatgc gtaccggctg aagggtgggg cggagctcgc cccgcttggc ttcgatgagc
    12121 tcatgaagga ctcggggacg ctcgtcgtgc tcgaatggcc ggagttggtc gcggacgggc
    12181 tccctgtagc cgacgtttcc atacagctct ccgtacagcc ggacgaaacc cgtctcatga
    12241 cctatggcta aggcgaagaa ggagaagaag atccggctcg tactgcttga tgcgcatgcg
    12301 atcatccacc gcgcgtacca cgcgcttccg gaattcaccg gccctgacgg ccagccgacc
    12361 ggtgccctat acggcctctc ctccatgctg ttgcggatca tgggggaact gaagcccgac
    12421 tacgtgctcg ctgcatacga ccttgcgggc cccacggtgc gccacgaggc ctacgaggac
    12481 tacaagggca ccaggcagaa gacggatgat gcgctcatcg cgcagctcga tacttcccgt
    12541 cgcgtgttcg aggctttttc ggttcccatt tacgagcgcg aggggttcga ggccgacgac
    12601 atcctcggca ccatcgtgaa gctcgagaag aagaaccagg atatcgagat catcatcgcg
    12661 tcgggcgaca tggataccat gcagctcatc gacggcaagc gggtgcaggt ctatacccta
    12721 aagaagggat tgaacgacac tatcctctac gacgaagagg ccgtgaagga gcgctatggc
    12781 ttcccgccgg aactcatccc ggactacaag ggcctgcgag gagacccctc ggacaacatc
    12841 aagggggtgc ccggcatcgg cgagaagctc gcgaccgagc tcgtgcagaa gttcgggagc
    12901 atggaggatc tgtacaagaa actcaagaag gacgaagcag cgctccttgc cgccggcatc
    12961 aagccgcgga tcgtgaatct cctcaaggag cacgaggagg acgccttctt ctcgaagatg
    13021 atcgcgacga tacgcaccga cgccccgatc gcctaccgcc tcccggagaa agactggaac
    13081 gagtgcatca cgctccccaa ggtgctcgcc ttgttcgatg agctcgggtt caggtcgctc
    13141 cgcccccgcg cggtctcgct cttcggcgag cagggcgcgg agtacgagga gctgccggcc
    13201 gaggagtcgg tcgcttccga ccggctcgcg caggcgaagg tgatgctctg gctcctccac
    13261 tcggacttca ccaacccgac cctggacgac gtcctcgcgt acacgaaggc caagaacttc
    13321 gatgaggcgt acgccaccct cgagaagaac cttgcggaga ccgggcgcct gaagcaggtg
    13381 tacgacgaga tcgagcggcc cctgatcgac atcgtccggg agatgggcga gtgcggcgtg
    13441 aagatcgata cggcggtcat ggaaagcctc aggaagaaat accgcgccga gctcgagggg
    13501 atcgagcagg gcatctatgc gatggtcggg cacgagttca acatcgcctc cccgaagcag
    13561 ctcggcgacg tcctcttcga cgagctccag ctcgtggtga agggccagaa gaagaccgcg
    13621 ggcggccagc gctcgacgaa ggaatccgaa ctcgagaaga tccgcgacct ccatccgatc
    13681 gtggacgagg ttctgcggta ccgcagcctc gggaaattgc tcgggaccta tatcgaggcg
    13741 atcccgcccc tcttggatag cgagtcccgg ctgcacgcgg agttcctcca gacgggcgcc
    13801 gtcacgggcc gcatggcgag ccagaacccg gggctccaga acatccccat caagacgccg
    13861 gagggccgcg cgatccgcaa tgccttcatt gcacgcgatg gctcggtgct ggtcgcgctc
    13921 gactattccc aaatcgagct ccggatcgcc gccatcctct ccaaggacga gaagctcatc
    13981 gagatcttca agtcggggcg tgacgtgcac cgcgaagtag cggccgcggt gttcaacgtc
    14041 gagccggagc tggtggacgc cgagatgcgc cgccgcgcga aggtcatcaa cttcggcatt
    14101 ttgtacggta tgggcgtgaa tgcgctgaag gcccagctcg gggtgccgct ctccgaagcg
    14161 cacgccttcc acgatgacta cttccagacc ttcgatacgc tcgcgaagta cctcgagtca
    14221 acccgcggat tcgcgcgcaa gcacggatac accgagaccc tgttcggcag gcgccgccag
    14281 ttcccggaga tgaaatctcc gctgccgtac gtgcgcgcgc aggccgagcg catggcgatc
    14341 aacgcgccca tccagggcac gcaggcggac atcattaaga aggcgatggt ggaggtggac
    14401 cggatgctgc atgaacgcgg gaagaaggac gacgcgcgtc tcatcctcca ggtgcatgac
    14461 gagctcgtgt acgagatacg ggaggaggac gcgaaggcgc ttgccgcgga catcaagcag
    14521 atcatggagg aggtgctccc cgggcaggaa gcgaacggcg tgcccatcat cgcggaaggg
    14581 aagctcggga agaactgggg ggagatgtcc ccactctaag ctcggccgct cagtaccgga
    14641 ggagcatact taatatggtt agtcggggag ttgcacatgt ccgagctcgc gttcaatact
    14701 tcgatcgttc cgttgacgga agacactgcg ccaagaggat cgcggccgcg gggttgggcc
    14761 atcgccgccg tcctctcgct cctcatctgg ttcagcccct acatcatctg gagcgccatc
    14821 cacgcgctca cctgatccac cgtcaccggg cacgatagga acgaggcgtt ccgcaaggga
    14881 cgcctccttc tatatactag tgaggtgatc tacctcttcc acggctcgga tgtccataag
    14941 gtgcgcgcga aggccttcca gtgggtcgcg gccgcgcgcg ccaaggcgcc ggacgccgcg
    15001 tacctccgcc tcaccgccga tcagctcacg agggaatctc tgctcgatgc cctcgcgtca
    15061 caggggctct tcttcgccaa gacgctcgtc ttgctcgatg atcccttctc cgagcgcgag
    15121 gcgggcgagc tcgtgctcga aaacctcacg gcactcaagg attccccgaa cccggtcgca
    15181 gtgctcgcgc cccggctcat cgcttcgagg gcgatgaagc tcgaggcggc tgccgagaag
    15241 gcattcaaga tcgatgcggt cgcgaagaag gagcgcgggt tcaacagcgc gctggtgaac
    15301 gcgcttgccg cacgcgatcg tctcctgctc tggaaggaga tcgtcaaagc gatgcgcgac
    15361 ggcgaagcgc cggaagccat acacggcctc ctccattgga aggcccgcga cctcatgcag
    15421 aaaggcggga gggcgtggac cccgaccgag gcccgccagc tctccgtctc cctcatcgaa
    15481 ctcctctcgg attcacgagg aagggatctg ccgctcccac tcgccctcga gcgctttgcg
    15541 ctcgggctat aatcgaactc tatgcataaa acactcatcg ccgtcgtcat cctcacgctc
    15601 gtaggcgccg ggctctacta cgtgctgacg caataccaac ccaatccgga atccgcttcg
    15661 gaccggcaga tgccgggcac tggcagcctc ttcagcacgg acggggaagt aaccggcacc
    15721 atcgtgctca tcaacacgga gcacgcggcg gtcgacggcc cctacgtcat caccgtccgg
    15781 caggcgggcg gcgagagcgc gatcatcgcg gtcccttcga tgggccttcc cacctgcgcg
    15841 gctcgcgaga agatcgcgga cgtgtatgcg ctccagccgg gccaggtagt gtcggcgcgc
    15901 ggcaccgtcg gcgagggcgg catcatcatc ccctgcgaat cgagcgagca cttcctctcg
    15961 ctcgtcgagt cgaactagtc ggtgcgcccg cctgggctcg aaccagggac cgtctcctta
    16021 aaagggagct gctctaccga ctgagctacg ggcgcaatgg aatcgttata ccgctaatag
    16081 gtgtacaaat ctaccgtcat tccttgcgcg tcgatgaggc ggacggcctc gcgtgacggc
    16141 ttccagagct tcgcattgcg ctcgaggtag atgcgccatt cctggctccg gaagtcctgc
    16201 tcgccctggt gcgcagcgac gcagccgttg taatcgaggt actcgtcgat gaactcctcg
    16261 cagcgattgg tgagccgcgg tcggccgcga tgcggcgggt tcgtgcactg cgggagggcg
    16321 gagaggtagt tgtagcagga gtcgtcggcg agcttgttac cctcgtagta gcgctcgaac
    16381 tccgcaagcg gggacgggca ggcgctcgag aggtggggcg agaaattctg gctcgtcccg
    16441 aaatacccca cgcagcggtt ctcccggaag gagacgccga tcggggagtc cccggtcgtg
    16501 atggtcgcgc ggtctcccgg ctggagcacg atcgggtccg tgcggttgat gccgcggcgc
    16561 gggagctccg ttcccccagg aatagttgct gacttgtagg tcgcatcgct ccggaggcgc
    16621 cagccggaga tgctgaccgg ccccggcgcg ttctgcgaga gctggagggt gacatactcg
    16681 tcctttgcac tgctccccgg ggtcacgctc gatatcgtta cctggccgcg cagcggggag
    16741 gcgttcgcgt atgcgtcgcc tgcctgcagg ttcgtcccgc cgaagaggtt gaagtccggc
    16801 gagaagaagg gctcgtcgct gtcgtaggcg aactgggtat cgtccacgtc cgtgatcggg
    16861 gtgatatagg ggccggcaaa cgagatgggc ctggtcggtc cgcccgagac gaaccagagg
    16921 aggaagaaga agaggaagat ggccaggaag aggatgccgt cgctcatgca gatatggtat
    16981 caggctcctc ccgcgagttt cttgagttcc tcacgcacca cggccgcatc gctccagggg
    17041 gtcttcttcc cgttggcttc catgatgtag ggatcggtgc ccttgccggt gatgaggacg
    17101 gcatcgcctg gacgagcgag cgagagcgca gcgccgatcg cctcgcgccg atccatgatg
    17161 atcttcgggg cccgcttcat gccagccgcc atcgcctcca cgatcgcgcg cgggtcctca
    17221 tcgtaagggt cctcgttcgt gaggatgacc tcgtcgcagg cctcgtccgc gatccggccc
    17281 atctccgggc gcttccaggt atcgcgcccg ccgcccgtgt tgccgaggac gcagatcttg
    17341 cggcccgggt acgcgctata gagggcggca agggagtcag gggtatgcgc ataatccacg
    17401 accgcttgga aaccctggcc ctcctcgatg cgctcgg
//
LOCUS       DASPKG010000043        11488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_752381, whole genome shotgun sequence.
ACCESSION   DASPKG010000043 DASPKG010000000
VERSION     DASPKG010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11488)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11488
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752381"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VEA36_01615"
     CDS             <1..726
                     /locus_tag="VEA36_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HYF29046.1"
                     /translation="AFITGKPVEKGGSAGRDTATADGAHAVLIALLADQHRSLQELRA
                     AVQGAGNAGAQAARLIERDGGRVVAIADSHGTIHNPAGLDVEMALHAKELHGSVCNAK
                     EDEHAEMRSPEAVITTPCDILVPAALEEQVRADNVEDVKTDIVLEIANGPVTPEADDI
                     LARRNVAVVPDVLANAGGVTVSYFEWLQNRAGESWTGEQVAARLHETMRDAYRDVADF
                     ALDRGVTLRQAAYALAVQRILNP"
     gene            complement(762..2741)
                     /gene="rnr"
                     /locus_tag="VEA36_01620"
     CDS             complement(762..2741)
                     /gene="rnr"
                     /locus_tag="VEA36_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HYF29047.1"
                     /translation="MAEKQETYEGPITITRTGKGFFTYDPEKPDIYIPPENLSGAFPN
                     DTVRVETAGFEFDPKTREKRAIGKVVEVIQRSRETFVGTLFQNKEENLVMLQPDWKKM
                     YVPFVVKGESLPLGHKVLIRFKGWAATQPYPWGQLEEIIGPAGEHETEMRALALSSGF
                     RSDFPPGVQEEAKKLEVEGKALIEAEAAAAIKDDRTKRGRHDFRGVTTFTIDPYDAKD
                     FDDALSVKKLDNGNVEVGIHIADVSYFVRPGTSIDTEARKRATSVYLVDRTIPMLPEV
                     LSSNYCSLNPNEDRLAVSAVFELDAQAGIISRWFGETVIHSDKRFTYEEAQEVLDNQD
                     GIFLEELNILKGLADQIRDRRTKKGAIAFDTPEVKIKLDETGKPIAVMLKERRDTNLL
                     IEDFMLLANEAVAEMLAEATKKANITNSVIYRIHDNPDPDRIENLAAFLKVMGYDLAM
                     HAGHVKGTDINQLLEKVAGTPEEYLIKTATLRSMAKAVYTTKNAGHFGLAFKYYTHFT
                     SPIRRYPDLLIHRLVKHYTNDGTFSPQQVAELDQLAFHSSEREAAAVEAERDSIKMKQ
                     VEFLAERIGEEFDAVISGVSDRGLYVELNETRAEGMIRIRDIGDDYFIYDEKRYRLVG
                     ERTKTEYALGDPIRVKLMDARLAERELDFALVAKQ"
     gene            2822..4087
                     /gene="purD"
                     /locus_tag="VEA36_01625"
     CDS             2822..4087
                     /gene="purD"
                     /locus_tag="VEA36_01625"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HYF29048.1"
                     /translation="MPTDNLIILVLGEGAREHALYGFNSESPSVKRVLCAPGNGGIDD
                     CDRRRFKTYQHDVLEYVCRTEGVDLVVVGPEAPLSAGVVDFLARHGIAAFGPTQAASI
                     LETSKAFTKLLCAEEGIPTAPFEVHENVGEAGLYIQSLAEPPVVKASGLCAGKGVVVA
                     DSHKEAIDAAFDMLQKRIHGDAGSTIVIEKRLSGRECSVMALCDGVNAVLLPPARDYK
                     RVGDGDTGPNTGGMGAISPVPDVDEALLERIKEEIILPTLRAMHKRGTPFHGLLYAGI
                     MLTEEGPMLLEYNVRFGDPETQVVLPRIESDLVPYLLATRTEGGLADLPPLQVSEDVM
                     IGVTLAMEGYPGKYKTGVPIRQDDSPVFIAGAELRDDGMLYTTGGRIATVVAGGATFA
                     EAREAVYRQIEGFNFDGRFYRTDIGADVV"
     gene            4717..5160
                     /gene="rpsP"
                     /locus_tag="VEA36_01630"
     CDS             4717..5160
                     /gene="rpsP"
                     /locus_tag="VEA36_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HYF29049.1"
                     /translation="MIRFQRIGRKNDPAFRMAVLEKTAGPKAGKYVDLVGTYNPKTKA
                     MSVDGDRIKEWVRKGAQLSPSLSNLLIAKGIIEGKKVNVLPKKTRIVKEQSAEAAAPA
                     PVAEEATAVVEETGEEVAVAEVLDAPGAVEETIAEDAKNEETPAA"
     gene            5232..5528
                     /locus_tag="VEA36_01635"
     CDS             5232..5528
                     /locus_tag="VEA36_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HYF29050.1"
                     /translation="MEMDHAFLDYVIKALVDNPDAVVITRTVDEMGVLLTLTVHPDDM
                     GKIIGRDGNIAKALRTLLRVVGMKHNARVNLKINEPVGGKRDGDRSLDDVVGEL"
     gene            5532..5951
                     /locus_tag="VEA36_01640"
     CDS             5532..5951
                     /locus_tag="VEA36_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29051.1"
                     /translation="MVDHLAEVRALNLPLGTYIVAGGSCLDVKGIRTSDDIDIVVTPE
                     VFEELKSRGWQVDLPFKEKWNRERLKHGYFEVYTDLSLENGHYTPAAEIIPKAELIEG
                     LAFMRLDELIYWKRINGRPKDLEDIRMIEAYLQKTNA"
     gene            5944..6606
                     /locus_tag="VEA36_01645"
     CDS             5944..6606
                     /locus_tag="VEA36_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HYF29052.1"
                     /translation="MLKFHLITLFSEACDAYLNASILGRARKEKKISVAYQSPRTFVK
                     NRWGKVDERPYGGGPGMVMSALPVVKAVKKALGRGTGKSVIVCFSPSAKQFTNKDADA
                     LAKFDRITFVCGRYEGIDARAKAMLKTLAPVKEYAVGEAVYTGGEVPALAMVDAITRR
                     LPGVLGKDESVEERRIASPDVYTRPEIIEWEGKVYRVPKVLLTGHHAKIDAHRSKKAK
                     TK"
     gene            6715..7125
                     /locus_tag="VEA36_01650"
     CDS             6715..7125
                     /locus_tag="VEA36_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29053.1"
                     /translation="MSYFAQEWPFAYFGFFVFLFGAFALWYGLETKFTGKTAIAFAPV
                     ILFVPLLAAELLGKHEGLAVFGGLLSGYVVAGVALLVYYMRTDPARDIQPDCASDPVP
                     LEPGVRVEARPMHGLPPKRRSLWRKRLARMLAHP"
     gene            7281..7553
                     /locus_tag="VEA36_01655"
     CDS             7281..7553
                     /locus_tag="VEA36_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29054.1"
                     /translation="MTGVQRDPHIDMVAFISANWGWGAYVSAFLLLGVLVVLAKQPEF
                     SRRRRPKSARRWKTKRVIEINGSFPDRKRGNGGVSVSAADRRIEYL"
     gene            7817..10990
                     /locus_tag="VEA36_01660"
     CDS             7817..10990
                     /locus_tag="VEA36_01660"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HYF29055.1"
                     /translation="MQKKLTAKTFSAYKAPRVEFPDLVGHQRSSFDWLLKDGLGELFK
                     EFSPIPDYSGKKFELRFEGFEVGDPKGTEEEARENMRTYEAPVKLTVTLLNKTLGTEK
                     TQEIFLADIPMMTPHGSFIVSGVERAIVPQLARSFGAFFVSEDIKGKQWFGAKLIPAR
                     GVWIEIESDADGAIYVKIDRKRKFPVTNLLSVFGGGLRDDLLKHFKGDETAIAAVKAT
                     LAHDTVENLDDAYVEIHRRMRDGDLATPDNAKQYVNALFAPERYDLSKVGRHRLNARF
                     NLPTDKKSLEKRALGLEDLVRIVAEIARLNVAPDARPDDIDHLGFRRVRFVGELLQAR
                     MRVGMSRMKRNIQDRMSTIESETSLPMAFVNPRPLQASIREFFTANQLSQFMDQQNIL
                     AELENMRTLSALGPGGLTRERAGFEVRDVHPSHYGRLCPIHTPEGANIGLILRMALHA
                     RTNEFGVLETPYAKVEKGMVTDKVEYLNALDEEEYVIAHGATPVDEKGRIIPDTIEAR
                     RGGEPAVVHREEIQYIDASTYQMFSAATAMIPFVDHDDANRALMGSNMQKQATPVLVP
                     EAPLVATGIEEHAARNTGRLVVAEESGEVTYADANRIVVTPANGKPHEYKLQGLTQTN
                     QNSAFSQRPIVRTGDKVKKGEVLADASSSDQGQMALGQNVRVAFMSWSGANYEDAIIV
                     SERLVEQSKFTTVHIEDFEAAVRDTKLGPEVTTHDIPNVGELRLKNLDEEGVVRIGAE
                     VRPGDILVGKVTPKGETQLTPEERLLRSIFGDKAKDVKDTSLRMEGGKRGRVIGVRVF
                     SREKGDQLESGIIKKIVVTVAQVRNVSVGDKLAGRHGNKGVISRVLPVEDMPYDKDGN
                     PVDIILTPLGVPSRMNLGQILELHLGLAAHTLGYQAIVPPFAGASADEIRDELEKAGF
                     DRSGKMQLYDGRSGEAFAQAVSVGYMYILKLHHMVEDKIHMRSIGPYSLITQQPLGGK
                     AQNGGQRFGEMEVWALLGYGSAYTLREMLTIKSDDIQGRSATFDAIVKGEPVSHAGTP
                     ASFSVLTNQIRGLALDVEPLADADLDA"
     gene            11010..>11488
                     /locus_tag="VEA36_01665"
     CDS             11010..>11488
                     /locus_tag="VEA36_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HYF29056.1"
                     /translation="MAPSNFNKRPQISRGQDWNALRLSLASPERILEWSRGEITKPET
                     INYRTQRSEKHGLFDEKIFGPERDYECYCGKYKGIRYKGIICDKCGVEITRSIVRRER
                     MGHIELVTPVAHIWFLRSMPSRMATIMGVPSADLEKVVYFAGYIVTKVNEETKKRLVA
                     "
BASE COUNT         2133 a         3622 c         3673 g         2060 t
ORIGIN      
        1 gcgttcatca cgggaaagcc cgtggagaag ggcgggagcg ccgggcgcga taccgcgacc
       61 gcagacggcg cgcacgcggt cctgatcgcg ctccttgcgg accagcatcg ctcgctccaa
      121 gagctgcggg ctgcggtgca aggggcgggg aatgcgggcg cccaggcggc gcggcttatc
      181 gagcgcgatg gcgggagggt cgtcgcgatc gcggacagcc acggcacgat ccataacccc
      241 gcgggcctcg acgtcgagat ggcgctgcat gccaaggagc tccatggctc ggtctgcaat
      301 gcgaaggagg acgagcatgc cgagatgcgc tcgccggagg cggtcatcac gacgccctgc
      361 gatatcctgg tgcccgccgc cttagaggag caggttcggg cagataatgt cgaggacgtg
      421 aagacggata tcgtgctcga gatcgcgaac ggccccgtga cccccgaggc ggacgatatc
      481 ctcgccaggc gcaacgtcgc cgtcgtcccg gacgtgctcg cgaacgcggg cggcgtgacc
      541 gtttcgtact tcgaatggct gcagaaccgg gcgggggagt cgtggacggg cgagcaggtc
      601 gcagcgcgtc tgcacgagac gatgcgcgat gcgtaccgcg atgtcgcgga cttcgcgctc
      661 gatcgcggcg tgacgctgcg gcaggcggca tatgcgcttg ccgtgcagcg catcctcaac
      721 ccctagctag cgtgctccct gcgagggtct ccgtagcggt gctattgctt cgccacgagc
      781 gcgaaatcga gctcccgctc ggcgagccgc gcgtccatga gcttcacgcg gatgggatcc
      841 ccgagcgcgt actcggtctt cgtgcgttcg cccaccaggc ggtaccgctt ctcgtcgtat
      901 atgaaatagt cgtcgccgat gtcccggatg cggatcatgc cttcggcgcg ggtctcgttg
      961 agctccacat agaggccgcg gtccgagacg ccggagatga ccgcgtcgaa ctcctcgccg
     1021 atgcgctccg cgaggaattc cacctgcttc atcttgatgg agtcgcgctc ggcttcgacc
     1081 gctgccgcct cgcgctcgga ggagtggaag gcgagctggt cgagctcggc gacctgttgc
     1141 gggctgaagg taccatcgtt ggtgtagtgc ttcacgaggc ggtggatgag gagatcgggg
     1201 taccggcgta taggggaggt gaagtgcgta tagtacttaa aggcgagccc gaaatgcccc
     1261 gcgttcttcg tggtgtagac ggccttcgcc atggagcgga gcgtcgccgt cttgatgagg
     1321 tactcctcgg gcgtgcccgc gaccttctcg aggagctggt tgatgtcggt gcccttcacg
     1381 tgccccgcat gcatcgcgag gtcgtagccc atgaccttga ggaaggcggc gaggttctcg
     1441 atgcgatccg ggtccgggtt gtcgtggatg cggtagatga cggagttcgt gatattcgcc
     1501 ttcttcgtgg cttcggcgag catctccgcg actgcctcgt tggcgagcag catgaagtcc
     1561 tcgatgagga ggttcgtgtc ccggcgctcc ttgagcatga cggcgatcgg cttgccggtc
     1621 tcatcgagct tgatcttcac ctcaggcgta tcgaaggcaa tcgcaccctt cttggtgcgt
     1681 cgatcccgga tctggtcggc aaggcccttc aggatgttga gctcttcgag gaagatgccg
     1741 tcctggttgt cgagcacctc ctgcgcctct tcgtacgtga agcgcttgtc ggaatggatg
     1801 acggtctcgc cgaaccagcg agagatgatg cccgcttgtg catcgagctc gaagacggcc
     1861 gagaccgcga gccggtcctc gttggggttg agggagcagt aattgctcga gaggacctcg
     1921 ggaagcatgg gaatggtgcg gtcgacgagg tagaccgagg tcgcgcgctt cctcgcctcg
     1981 gtatcgatag acgtgccggg acgcacgaag taggagacgt cggcgatgtg gatgccgact
     2041 tcgacgttgc cgttgtcgag cttctttacg gaaagggcat catcgaagtc cttcgcatcg
     2101 tagggatcga tggtgaaggt ggtgacgccg cggaagtcgt ggcggcccct cttcgtgcgg
     2161 tcgtccttga tcgctgcagc ggcctcagct tcgatgagcg ctttgccttc gacctcgagc
     2221 ttcttcgcct cctcctgcac gccgggaggg aagtccgagc ggaatccgct cgagagggcg
     2281 agcgcgcgca tctcggtctc gtgctcgccg gcaggtccga tgatctcctc gagctgtccc
     2341 cacggatagg gctgcgtggc cgcccatcct ttgaagcgga tgaggacctt gtgccccaag
     2401 ggcagggact cgcccttcac cacgaagggc acgtacatct tcttccagtc cggctggagc
     2461 atgacgagat tctcctcctt gttctggaag agggtaccga cgaaggtctc gcgggaacgc
     2521 tggatgacct ccaccacctt cccgatggcg cgcttctcgc gggtcttggg gtcgaactcg
     2581 aagcccgcgg tctccacccg gaccgtatcg ttggggaagg cccccgagag attctcgggc
     2641 gggatgtaga tgtcgggctt ctcgggatcg taggtgaaga atcccttgcc ggtccgcgtg
     2701 atcgtgatcg gtccttcgta cgtttcctgc ttctcggcca tttccctaag actgtagcag
     2761 atacggctgt atatgctatg aataccccgg actggaacct ccgagacgaa aggattcgga
     2821 aatgcccact gacaacctca tcatcctcgt gctcggggag ggcgcccgcg agcacgcgct
     2881 ctacggcttc aacagcgaga gccccagcgt caagcgggtg ctctgcgcgc ccggcaacgg
     2941 cggcatcgat gactgcgatc gccgccgctt caagacctac cagcacgacg tgctggaata
     3001 cgtctgccgc accgagggcg tcgacctcgt cgtggtcggg cccgaggcac cgctttccgc
     3061 gggcgtggtg gacttcctcg cgcggcatgg catcgccgca ttcggaccga cccaagcggc
     3121 gtccatcctc gagacgagca aggccttcac gaagctgctc tgcgccgaag aaggcatccc
     3181 gaccgcgccg ttcgaggtcc atgagaacgt cggcgaggcc gggctctaca tccagagcct
     3241 tgccgagccg ccggtcgtga aagcgagcgg gctgtgcgcc ggcaagggcg tcgtcgtcgc
     3301 tgactcgcac aaggaggcga tcgatgcggc gttcgacatg ctccagaagc gcatccacgg
     3361 cgacgcgggc agcacgatcg tcatcgagaa gcggctctcg ggccgcgagt gctcggtcat
     3421 ggcgctctgc gatggggtga acgcggtcct gctgccgccg gcgcgcgact acaagcgcgt
     3481 cggcgacggg gacacgggcc cgaatacggg cggcatgggc gccatatcgc ccgtgccgga
     3541 cgtcgatgag gccctgctcg agcgcatcaa ggaggagatc atcctcccga cgctgcgcgc
     3601 gatgcataag cgcgggacgc ctttccatgg gctcctctac gccggcatca tgctgaccga
     3661 agaagggccg atgctcctcg agtacaacgt gcgcttcggc gaccccgaga cgcaggtggt
     3721 gctgccgcgc atcgagtccg acctcgtccc gtacctgctc gcgacgcgta cggaaggcgg
     3781 cctcgccgac ctcccgccgc ttcaggtgag cgaggacgtc atgatcggcg tcacgctcgc
     3841 gatggaaggg tatcccggca agtacaagac cggcgtgccc atccggcagg atgactcgcc
     3901 cgtcttcatc gccggagccg aactgcgcga tgacggcatg ctctacacga caggcggccg
     3961 catcgcgacc gtggtcgccg gtggcgccac cttcgccgaa gctcgcgaag cggtgtaccg
     4021 ccagatcgaa ggattcaact tcgacggcag gttctaccgt acggatatcg gggcggacgt
     4081 cgtctgagcg gcttctcgat ctggatttgg tcccggctgt ggtgatggcc gggattcttt
     4141 ttatcccctg acgcgcatta gagactcttt tcactgctat gcttaacggt gcagcatgtc
     4201 cgctgcaccc caaccccccc caacttcggc ggcccttcgg ggccgtcttt ttcatcccgc
     4261 ctttagcgag ccaggcctat gctcgagaaa gcctcccact ctcccggatg cctgcgaagc
     4321 gataggaaag agaaggagga accctgaact acagcgatct ctagggcttc gggaccgctg
     4381 tatttttatg ctatacattc cacagcgctc gacctggtcc tatattccag cagggaaggc
     4441 gctggccgtc gcgatgacgc ccgaactcct tggtatggct ccaagcctct cgcgggcccc
     4501 ggcccgcgag agcgcccgcc cggcacatgc ccgggcgttt ctggtatagt gttgaggtgc
     4561 accggttggt gccacaatcc tgagaccgta ctacccctgg ccctcgacca tcatgagggc
     4621 ctcccaaagg tgcggcgggc ccatcctctg gcccgccgca ttttcttttc attgacccgt
     4681 tttagccttt ctgttacagt tccagccact atgttgatga tccgattcca gcgcattggc
     4741 cgcaagaacg acccggcctt tcgcatggcc gtgctcgaaa agaccgcagg acccaaggcc
     4801 ggcaagtacg tcgacctcgt cggcacctac aacccgaaga ccaaggccat gagcgtggac
     4861 ggggaccgca tcaaggagtg ggtgcgcaag ggcgcacagc tttcgccgtc cctttcgaac
     4921 ctcctcatcg ccaagggcat catcgaagga aagaaggtga acgtccttcc caagaagacc
     4981 cgcatcgtga aggagcagtc ggctgaagcc gcagctcccg ctccagtagc cgaggaggca
     5041 accgccgttg tcgaggagac cggcgaggag gtcgcggtcg ctgaagtcct cgacgccccc
     5101 ggggccgtcg aggagacgat cgccgaggac gcgaagaacg aggagacccc ggctgcgtaa
     5161 gcagtccgct cgggcttctc ccgtccttca tccgtctttt ctgatattct gtatctaact
     5221 gcacccctgc tatggaaatg gaccacgcat tcctcgacta cgtcatcaag gcgctcgtcg
     5281 ataacccgga cgccgtcgtc atcacccgca ccgtcgacga gatgggcgtg ctcctgaccc
     5341 tcacggtgca cccggacgac atgggcaaga tcatcggccg cgacggcaac atcgcgaagg
     5401 cgctgcgcac gctgcttcgg gtcgtcggca tgaagcacaa cgcgcgcgtg aacctcaaga
     5461 tcaacgagcc ggtgggcggc aagcgcgatg gcgatcgttc gctcgacgac gtagtcggtg
     5521 agctctaacg catggtggat catctcgcgg aagtacgtgc gctcaatctt ccgctcggga
     5581 cctacatcgt tgccggcggg tcgtgcctgg atgtgaaggg tattcgtacg agtgacgata
     5641 tcgatatcgt cgtgaccccg gaggtattcg aggaactcaa atcgcgcggc tggcaggtcg
     5701 atctcccgtt caaggagaaa tggaaccgcg agcgtctcaa gcacgggtat ttcgaagtgt
     5761 atacggacct cagccttgag aacggccact acacgcccgc tgcggaaatc atcccgaaag
     5821 ccgagctcat tgagggactc gcattcatgc gtctcgatga actcatctac tggaagcgga
     5881 tcaatggccg accgaaggac ctcgaggata ttagaatgat cgaagcttat ttacagaaga
     5941 cgaatgctta aattccacct catcaccctc ttctcggaag cctgcgatgc atatttgaat
     6001 gcgtcgatac tcgggcgcgc ccgcaaggag aagaagatca gcgtggcgta ccagtcgccg
     6061 cgcacctttg tgaaaaaccg ctgggggaag gtggacgagc gcccgtacgg cggggggccc
     6121 ggcatggtca tgtccgcgct cccggtcgtg aaagcggtga agaaggcgct cgggcgcggg
     6181 acggggaagt ccgtcatcgt ctgcttctcg ccctcggcga agcagttcac gaacaaggac
     6241 gcggacgcgc ttgcgaagtt cgatcgcatc acatttgtct gcggacggta cgagggtatc
     6301 gacgcgcgcg cgaaggcgat gctcaagacg ctcgcgcccg tgaaggagta cgcggtcggc
     6361 gaggcggtgt acacgggcgg ggaagttccc gcgctcgcga tggtggatgc gatcacgcgg
     6421 cgcctccctg gcgtcctcgg gaaggacgaa tcggtggagg agcggaggat cgcatcgcct
     6481 gacgtataca cccgtcccga gatcatcgaa tgggaaggga aggtgtaccg ggtgccgaag
     6541 gtgctcctga ccggccacca cgcgaagatc gacgcgcacc ggagcaagaa ggcgaagacg
     6601 aaataagcca tcaatatccc cacggtcggg atattgactt ttatatacga tagtgatata
     6661 gtactggccg aagcctggca cgttggggtg ctcaggtaca agggaacctt ggcaatgtcg
     6721 tatttcgctc aggagtggcc gttcgcctac ttcggcttct tcgtcttcct gttcggcgcg
     6781 ttcgcgctct ggtacgggct cgagacgaag ttcaccggga agacggcgat cgcgttcgct
     6841 cccgtcatcc tgttcgtgcc gcttctggcg gccgagctgc tcggcaagca cgagggtctc
     6901 gcggtcttcg gcggcctctt gagcggctac gtcgtcgccg gcgtcgcgct cctcgtgtac
     6961 tacatgagga ccgacccggc gcgggacatc cagccggact gcgcaagcga cccggttccg
     7021 ctcgagcctg gcgttcgtgt ggaagcgcgg ccgatgcacg ggctgccgcc gaaacggcgg
     7081 agcctctggc gcaagcggct cgcgcgcatg ctggcgcacc cctaagttcc gatccccgga
     7141 acgcggcggg cggtcttctc aaagaccgcc cgcttttcgt ttgtgcatat ccgatttgac
     7201 gagagcacga gcgaggctta ataattcgca gtctggtgtc gccttggcgc atcagacacg
     7261 ttcttagcga ggggagaggt atgacgggag tgcaacgaga tccgcatatc gacatggtgg
     7321 cgttcatctc cgccaactgg ggatggggtg cctacgtcag cgcattctta ctgctcggtg
     7381 tgctggtcgt gctggcgaag cagcctgagt tcagccgtcg ccggcggccg aagtccgcgc
     7441 ggcggtggaa gaccaaacgc gtgatcgaga tcaacggctc cttcccggac cggaagcgcg
     7501 gcaatggggg cgtatccgtc tccgctgccg atcgccggat cgaatacctc tagaatggag
     7561 gaactactaa gctcattacg gagggcggcc accagaacgg gggccgcctt catccattct
     7621 tttccccacc tgcgcttgcg ccctggagtg ggcaggagta tgatggcctc acgaatgagt
     7681 aacggctggg ggaggctggg ctaaggagta cagagggcga tttacaaact gcgtctcgga
     7741 aaacgagaga cgcggctcgt ggtgtgtcca cgagcccgtt tgccgttttc tagggacgcc
     7801 ataagaaccg cgcagcatgc aaaagaagct cacggcaaag acgttcagcg cgtacaaggc
     7861 accgagggtg gagttcccgg acctcgtcgg ccatcagcgc tcgtcgttcg attggctact
     7921 caaggacggg ctcggcgagc tcttcaagga gttctcgccc atcccggatt attcaggcaa
     7981 gaagttcgag ctccgattcg agggcttcga ggtcggggac ccgaagggca ccgaggagga
     8041 agcgcgcgag aacatgcgca cctacgaggc gccggtgaag ctcaccgtca cgctcctcaa
     8101 caagacgctt ggcaccgaga agacgcagga gatcttcctc gccgacatcc cgatgatgac
     8161 gccgcacggc tcgttcatcg tctcgggtgt cgagcgcgcg atcgttcccc agctcgcgcg
     8221 ttccttcggc gcgttctttg tctccgaaga catcaagggc aagcagtggt tcggcgctaa
     8281 attgatcccc gcccgcggcg tctggatcga gatcgagtcg gacgccgatg gcgcgattta
     8341 cgtgaagatc gaccgcaagc gcaagttccc ggtcaccaac ctcctctccg tcttcggtgg
     8401 gggccttcgc gatgacctcc tgaagcattt caagggcgac gagaccgcga tcgcggccgt
     8461 caaggccacc ttggcgcatg acaccgtcga gaacctcgac gacgcctatg tcgagatcca
     8521 tcgccgcatg cgcgatgggg acctcgcgac gccggacaac gcgaagcagt acgtgaatgc
     8581 cttgttcgct cccgagcgct acgacctctc gaaggtcggg cgccatcgcc tgaacgcccg
     8641 cttcaatctc ccgacggaca agaagtcctt ggagaagcgc gcgcttgggc tcgaggatct
     8701 cgtccgcatt gtcgccgaga tcgcgcgcct gaacgtcgct cccgacgcac gtcccgacga
     8761 catcgatcac cttggcttcc gccgcgtgcg gttcgtgggc gaattgctgc aggcccgcat
     8821 gcgcgtcggt atgtcgcgca tgaagcgcaa catccaggac cgcatgtcca cgatcgagag
     8881 cgagacctcg ctcccgatgg ccttcgtgaa cccgcgcccc ttgcaggcgt ccatccgcga
     8941 gttcttcacc gccaaccagc tctcgcagtt catggaccag cagaacatcc tcgcggagct
     9001 cgagaatatg cgcacgctct cggcgctcgg gcccggcggt ctcacccgcg agcgcgccgg
     9061 ctttgaggtc cgcgacgtgc atccgtccca ctacggccgc ctttgcccca tccacacgcc
     9121 ggaaggcgcg aatatcggcc tcatcctgcg catggcgctg catgcccgca cgaacgagtt
     9181 cggcgtgctc gagaccccgt acgcgaaggt cgagaagggc atggtcaccg acaaggtgga
     9241 gtacctgaac gcgctcgatg aggaggagta cgtcatcgcg catggcgcca cgccggtgga
     9301 cgagaaggga cgcattatcc cggacaccat cgaggcgcgc cgcggcggcg aaccggccgt
     9361 cgtgcaccgc gaggagatcc agtacatcga cgcctcgacc taccagatgt tctcggcagc
     9421 gaccgcgatg atcccgttcg tggaccacga cgacgccaac cgcgcgctca tgggctcgaa
     9481 catgcagaag caggcgaccc cggtcctcgt tccggaggcg cccttggtcg cgaccggcat
     9541 cgaagagcat gcagcccgca ataccggccg cctcgtggta gcggaggagt ccggcgaagt
     9601 gacgtacgcg gacgcgaacc gcatcgtcgt gacgcccgca aacggtaaac cccacgagta
     9661 caagctccag ggcctcaccc agaccaacca gaactcggcc ttcagccagc gccccatcgt
     9721 gcgcacggga gacaaggtga agaagggcga ggtcttggcc gatgcctcga gctcggacca
     9781 gggccagatg gcgctcggac agaatgtccg cgtggccttc atgagctggt cgggcgccaa
     9841 ctacgaggac gccatcatcg tctcggagcg cctcgtcgag cagtccaaat ttactaccgt
     9901 gcatatcgag gacttcgaag cggccgtgcg tgacaccaag ctcggtcccg aagtcaccac
     9961 ccacgacatc ccgaacgtcg gcgaactgcg cctcaagaac ctcgacgaag agggcgtggt
    10021 gcgcatcggt gccgaggtgc gccccggcga catcctcgtc ggtaaggtga cgcccaaggg
    10081 cgagacccag ctcacccccg aagagcgcct tctccgctcg atcttcggcg acaaggcgaa
    10141 agacgtgaag gacacctcgc ttcgcatgga gggcgggaag cgcggccgcg tgatcggggt
    10201 ccgggtcttc tcccgcgaga agggcgacca gctcgagagc ggcatcatta agaagatcgt
    10261 ggtgacggtc gcgcaggtcc gtaacgtgtc ggtgggcgac aagctcgccg gccgccacgg
    10321 caacaagggc gtgatctccc gcgtgcttcc ggtcgaggac atgccctacg acaaggacgg
    10381 caatccggtc gacatcatcc tcaccccgct tggcgtgccg agccgcatga acctcgggca
    10441 gatcctcgag ctccacctcg ggcttgcggc tcacaccctc gggtaccagg ccatcgtccc
    10501 gccgtttgcg ggagcctcgg ccgacgagat ccgcgacgag ctcgagaagg cgggcttcga
    10561 ccgctccggc aagatgcagc tctacgacgg ccgctcgggc gaagcgttcg cccaggcggt
    10621 ctcggtgggc tacatgtaca tcctgaagct ccatcacatg gtggaggaca agatccacat
    10681 gcgctccatc ggtccgtact cgctcatcac ccagcagccg ctcggtggta aggcgcagaa
    10741 cggcggtcag cgcttcgggg aaatggaagt gtgggcgctg cttggctatg gctctgccta
    10801 tacgctgcgc gagatgctca ccatcaagtc agacgacatc cagggacgct ccgcgacctt
    10861 cgatgccatc gtgaagggcg agccggtcag ccacgcaggt accccggcct ccttctcggt
    10921 gcttaccaac cagatccgtg gtcttgcgct cgatgtcgag ccgctcgccg acgcagatct
    10981 cgacgcctaa ccctacgact atgtcaccca tggcacctag caacttcaac aagcgtccgc
    11041 agatctcgcg agggcaggac tggaacgcgc tccggctttc gctcgcgtcc cctgagcgca
    11101 tcctcgagtg gtcgcgcggc gagatcacca agcccgagac catcaactac cgcacccagc
    11161 gctccgagaa gcacgggctc ttcgacgaga agatcttcgg ccccgagcgc gactacgaat
    11221 gctactgcgg caagtacaag ggcatccgct acaagggcat catctgcgac aagtgcggcg
    11281 tcgagatcac gcgttccatc gtgcgccgcg aacgcatggg ccacatcgag ctcgtcaccc
    11341 cggtcgccca catctggttc ctgcgcagta tgccgagccg catggcgacc atcatgggcg
    11401 tgccgtccgc ggaccttgag aaggtcgtct acttcgccgg ctacatcgtg accaaggtca
    11461 atgaggagac caagaagcgc ctcgttgc
//
LOCUS       DASPKG010000044         6206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_349903, whole genome shotgun sequence.
ACCESSION   DASPKG010000044 DASPKG010000000
VERSION     DASPKG010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349903"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4093)
                     /locus_tag="VEA36_01670"
     CDS             complement(<1..4093)
                     /locus_tag="VEA36_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYF29057.1"
                     /translation="MSSLLPDNLISTKEASEFSGYHSDYLARMCRAGKIEGRQVGRTW
                     LVSRESVEKFKQEQEERKRELAASLSKAREAEYQKVKNQDVKPVEVPAAPIAEAAPAP
                     VIEVIPASIPAPVHPTMAPARVERPQAPVKSPYAPSAFAHQALALSITIAALSIGTQL
                     ASTGLWNEGARLAIASVESAHVLGKSAYLAALERTGAGLFAFGTATRDLAINTDAVTA
                     FRAPGEFVVASVDAILDGYAFGTFRFAEKAPELPKYAVESTRALGTAISSMLATGIVT
                     TPDVYEHATNEAALATVEAAEGVIAFTDTATRAASIALAPADDVLEGAGGTATQSASA
                     VATFSPDAAAGVRDWFLGRAEHLARSTYGTFSIFYDWAGYAVASIMDPYTNRFPIVMV
                     DYPRAGGTTATSIVNRIGTTTIIQNYHQTAGEGASVAYVDMWVARLQRAIDTNFENIN
                     DNGTSGGGITEVSGDIDVTSITVTGNGTFGGNVTITGTLSAGSLAVGSLSTGLPIEAP
                     YFSATSTSATSTFLGGFYAKRVEAGDYLAGPYILATSTTATSVFSGNIAANGNTTIGD
                     ASSDTLTVNASIASSLIPSAAATYDLGSITSPWRTLFAESASTTYATSTSFATTNFRL
                     GGSLFTSLLGNGLTNSGGILAVSTTSLASGFFLNGGNSFGATGVIGTNDAQALQLETN
                     NLPRITIDSSGNVGVGTSTPGSLFSIANIANFTAATTSFYSTGGINLAGGCFAVNGTC
                     LGSGSGSGTVNSGTAGQIAYYDANGTAVSGTSSIFVAPSGNVGIGTTSPYARLSVVGQ
                     AVAEYFTATSTTATSTFPLADITNALRFAGDYFTDLTGTGLQNVGGALSINATGDWTG
                     TFDGQEGSYYLANSFSTSSAQFFVNGSTTIPKTYTANTFTGSQVFSGGVTIGTLNGPL
                     QANAGVVSATTSIGVLYGGTGLTTAPSYGNILVGNSSGGYTLTATSSLGLATAFTTTY
                     PIQNTNNVLSLAFGTTSNNTWSGTNNFIGNVGIGSSSPFARLSVHANADETATTLFAI
                     GSSTASATTTLFSVSNQGLTTLQVANSGGEANLLSLYNSSNGSTNSGSNLSFRTTNSN
                     GAAVNTARIASILTTATAGSEDSTLVFRTIVDGGLTERVRIHSDGNIGIGTTSPYAKL
                     SVVGQTVSEYFTATSTTATSTFPNLALTNLLFGSDYVTDLTGSGLSISNGALTIGTVG
                     QANGGTGFTTYTAGDILYADGSGNLAKLPIGGNGTVLKVAAGLPSWGTDLTSGGGGGA
                     GAWATTTDSLAVYPTDTTDVIIIGTNATTTSTSIFEVSGRSYFSSTVSVASTSPWGLL
                     SVNPNGITGPAFVVGSSTGTNFIVTDGGRVGIGTTAPAALLGVA"
     gene            complement(4494..5465)
                     /locus_tag="VEA36_01675"
     CDS             complement(4494..5465)
                     /locus_tag="VEA36_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29058.1"
                     /translation="MDELTIGDKIYISSKQAAKITGYAKDYVGQLCREGRVDAKLVGR
                     NWYVLESSIREHRFGKETLADVPQASHVEERISPVSAWEAPRYAPMEDEPAIPPLEAI
                     KPAPAVEDSAEAVALMQDAWKEWFASREQMQAEQEAVIEEATPEPIIEEPTKAYREEE
                     VSEEEPVRVSDIPSNYSVEKVSQDFEDEERIINMIRMEREVEHEPEIPHEPVMISRTR
                     MTPEPVYEAPRPRAQGSVIDLSRAHQEAYTAPQRPIARPTQVQRQHKTRTMLTAQALM
                     LSIAGLSILAAVIGSGALDNALASQGIESSLRGAIFDTLRGESTLKK"
     gene            complement(5557..>6206)
                     /locus_tag="VEA36_01680"
     CDS             complement(5557..>6206)
                     /locus_tag="VEA36_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HYF29059.1"
                     /translation="FSQRVKEGSADYRYFPEPDLPKLVLSELSWLADIQSSLPELPAE
                     KRARYEGLGLKPEHAAQFAGSIEDSAFFDAVLAESGGAPDRISIAANYLITDVAGAYD
                     NLTPKAFAQLIALIAENKISSRGAKDALALLREKGGESALLVVEEAGLLQVSDTESLR
                     KAVQEVLAANPAVAEEYRGGKESVLQYLVGQGMKATKGAGNPTVLRELIIEELGK"
BASE COUNT         1108 a         1787 c         2071 g         1240 t
ORIGIN      
        1 ctgcgacccc aaggagcgcg gcgggtgcgg ttgtgccgat gcccacgcga ccgccgtcgg
       61 tcacgatgaa gttggtgccg gtcgaggaac cgaccacgaa cgccggacct gtgatgccgt
      121 tcggattcac cgagaggagg ccccacgggg aggtcgatgc aacgctgacc gtgctcgaga
      181 agtaggagcg gccggagacc tcgaagatgc tcgttgaggt cgtggttgcg ttggtcccga
      241 tgataatcac gtccgtggtg tcggtcggat agaccgcgag gctgtcggtg gtcgttgccc
      301 acgcgcctgc cccgccgccg ccgcccgaag tgagatcggt gccccaggac ggaagtcctg
      361 cggcgacctt gagcaccgtt ccgttgccgc cgatcgggag cttcgcgaga ttgccactac
      421 cgtccgcata gagaatgtcg ccggcggtgt aggtagtgaa gcccgtgccg ccgttcgcct
      481 gcccgaccgt gccgatggtg agcgcaccgt ttgaaatcga gaggccggag cccgtgaggt
      541 ccgtgacata gtcgctgccg aagaggaggt tcgtgagcgc gaggttcggg aaggtcgagg
      601 tggcggtcgt cgaggtggcg gtgaagtatt cggaaacggt ctggccgacg accgagagtt
      661 tggcgtaggg cgaggtcgtg ccgataccaa tattcccgtc cgaatggatg cgtacacgtt
      721 cggtaagtcc gccgtcgaca atggtgcgga agacgagcgt ggaatcttct gatcctgccg
      781 ttgccgtcgt gagaatggac gcaattcgtg cggtattcac tgcagcaccg ttactgttcg
      841 ttgtccggaa gctgaggttg ctgccggaat tcgtcgaacc gttactgctg ttatagagac
      901 taaggaggtt cgcctcgccg ccgctattgg cgacctgcag cgtggtgagt ccttgattgc
      961 ttaccgagaa gagagtggtt gtcgcgcttg ccgtcgacga gccgatcgcg aagagcgtcg
     1021 tggcggtttc gtcggcattg gcatggacag agagccgtgc aaacggcgag gaggagccga
     1081 ttcccacgtt gccgataaag ttgttcgtgc ccgaccaggt gttgttgctt gtcgtcccga
     1141 acgcgagcga aagaacattg ttcgtattct gaatcgggta cgtagtcgta aacgcggttg
     1201 caagcccaag cgaagaagtc gcagtaagcg tatagccgcc tgacgagtta ccgacgagga
     1261 tgttgccata gctcggcgcg gtggtaaggc cggtgccgcc gtacaggacg ccgatcgacg
     1321 tggtcgccga gacgacgcct gcgtttgcct gcaaggggcc attgagcgtg ccgatggtca
     1381 ccccaccact gaatacctgc gagccggtga aggtatttgc ggtataggtc ttcgggatgg
     1441 tggtcgagcc attcacgaag aactgtgcgg aagacgtgct gaaggagttc gcgagatagt
     1501 agctaccctc ctggccatcg aacgtgccgg tccaatcgcc ggtcgcattg atggagagcg
     1561 cgccaccaac gttctggaga cctgtgccgg taagatcggt gaagtagtcg cccgcgaagc
     1621 gaagcgcgtt cgtgatgtcc gcgagcggga aggtcgaggt cgcagtggtg gaggtcgcgg
     1681 tgaagtattc cgccaccgcc tgcccgacga ctgagagccg tgcatagggg cttgtcgtgc
     1741 cgatgcccac gttgccgctc ggcgcgacga agatgctcga ggtgcccgag accgcggtgc
     1801 cattggcgtc gtagtaggcg atctgcccgg ccgtgccgga attcaccgtg cccgagcccg
     1861 aaccacttcc aagacaggtg ccgttcaccg caaagcagcc gcccgcaagg ttaatgccgc
     1921 ccgtggagta gaaggaggtc gtggcagcgg tgaagttcgc aatgttcgca atcgagaaga
     1981 gagagcccgg ggtcgaggtg ccgacgccca cgttccccga cgagtcgatg gtgatgcgcg
     2041 ggaggttatt ggtttcgagc tggagcgcct gcgcgtcgtt ggtgccgatg acccccgtcg
     2101 ccccgaagga gttcccgccg ttcaggaaga agcccgaggc gaggctggtg gtcgagacgg
     2161 cgagaatgcc gcctgagttc gtgaggccgt tgccaaggag cgaggtgaag agcgagccgc
     2221 cgaggcggaa gttggtggtc gcgaagctcg tcgaggtcgc gtaggtcgtg gaggcgctct
     2281 ccgcgaagag cgtgcgccag gggctcgtga tggagccgag gtcgtaggtg gcagccgcag
     2341 acgggatgag cgaggaggcg atcgaggcgt tcacggtcag cgtatcgctc gaggcgtccc
     2401 cgatcgtcgt gttgccgttg gctgcgatgt tcccggagaa gacggaggtc gcagtggtcg
     2461 aggtcgcgag gatgtaggga cccgcgaggt agtcaccggc ttccacgcgc ttcgcgtaga
     2521 agccgccgag gaaggtggag gtggccgacg tcgaggtggc gctgaagtag ggcgcttcga
     2581 tcgggagccc cgtcgagagc gagccgaccg caagcgaacc agcggacaag gtaccggtga
     2641 tggttacgtt gccaccgaag gtaccgttac cggtgacggt gatcgacgtg acgtcgatgt
     2701 cgcccgagac ctccgtgatg ccgccgcctg aggtcccgtt atcgttgatg ttctcgaagt
     2761 tcgtatcgat cgcccgctgc aggcgcgcca cccacatatc cacgtacgcg accgaggcgc
     2821 cctcgcctgc ggtctggtgg tagttctgga tgatggtcgt ggtgccgatg cggttcacga
     2881 tgctcgttgc ggtcgtgccg cctgcgcgcg ggtagtcgac catgacgatc gggaaccggt
     2941 tcgtgtacgg gtccatgatc gatgcgaccg cgtagcccgc ccagtcgtag aagatcgaga
     3001 aggtgccgta ggtgctgcgc gcgaggtgct cggcgcgccc gaggaaccag tcgcgcactc
     3061 ctgcagccgc gtccggcgag aaggtcgcga ccgcggacgc cgactgcgtc gcggtacctc
     3121 ccgcgccctc gaggacgtcg tctgcgggag caagcgcgat cgaggcggcg cgggtcgcgg
     3181 tatcagtgaa cgcgatgacg ccctcggcgg cctcgacggt ggcgagcgct gcctcgttcg
     3241 tcgcgtgctc gtacacatcg ggcgtcgtga cgatgccggt cgcgagcatc gaagagatcg
     3301 cagtgccgag cgcgcgcgtg gactcgaccg cgtacttcgg gagctcgggc gccttctctg
     3361 caaagcggaa ggtaccgaag gcgtacccat cgagaatggc atcgaccgac gcgacgacga
     3421 actcgcctgg cgcgcggaac gcggtgaccg catccgtgtt gatcgcaaga tcgcgggtcg
     3481 cggtcccgaa cgcgaagagg cctgcgccgg tcctctcgag cgccgcgagg tacgcggact
     3541 tgccgagcac atgcgcggac tcgacggagg cgatcgccag ccgcgcgccc tcgttccaga
     3601 ggccggtcga ggcgagctgc gtgccgatcg agagcgccgc gatcgtgatg gagagcgcga
     3661 gcgcctggtg cgcgaaggcg ctcggcgcgt acggagactt gacgggcgcc tgcgggcgct
     3721 cgacacgcgc cggcgccatg gtcgggtgca cgggcgctgg aatcgatgca ggaattacct
     3781 cgatgacggg cgccggtgct gcttcggcaa tgggcgcagc cggtacctcg accggcttga
     3841 cgtcctgatt cttgactttc tgatattccg cttcgcgcgc cttcgagagc gatgcggcga
     3901 gttcgcgctt acgctcctcc tgctcctgct tgaacttctc gacggattcg cgcgagacga
     3961 gccaggtgcg tcccacctgc cgtccctcga tctttccggc gcggcacatg cgcgcgaggt
     4021 agtcggaatg gtacccggaa aattccgatg cctctttggt ggagatgaga ttgtctggaa
     4081 ggagcgaaga catgtcggat atccgaatta cttatatttt atctgtgata tcggaaatac
     4141 tgcagagatt tttgtggata tcattgaaga gctttttcag tgcggctcgg agaaatttct
     4201 ctccgcgatg atcaaaaaaa tccgcggctg agggtcatgc gcagaatgat ggcgaagggg
     4261 tgatagagat gaaaaagaga gtctcgcgca ggacactacg tgaggctcaa aatggctcgt
     4321 gacgcgtttt gattctcaga gagccgctat cgtgcagtca gcgtgcacgg ctcacgtgcg
     4381 aaagtgcgtt gaaattgctc gtgaacgtcg aggatgcgaa aaatctccgc atcaacggag
     4441 acttttcctg ttatgagact cgtcttggcg agacgagaaa tatagataaa ctcttacttt
     4501 ttaagcgtgc tctcgccacg aagagtgtcg aatatcgctc ctcgtaggct actctcaatt
     4561 ccctgtgagg caagcgcgtt atcgagtgcg ccgctcccaa tgactgccgc gagtattgag
     4621 aggcctgcaa ttgagagcat aagcgcctgc gcagtgagca ttgtgcgtgt cttgtgctgc
     4681 cgctgaacct gcgtgggacg tgctattggc ctctgtgggg ctgtatacgc ctcctggtgc
     4741 gcgcgggaga gatcgatgac acttccctgc gcacgcggac gcggtgcttc gtatacgggc
     4801 tcgggcgtca tgcgcgtacg cgagatcatc acgggctcgt gcgggatctc cggttcgtgc
     4861 tcgacttccc tttccatgcg gatcatattg atgattcgct cctcgtcctc gaaatcctgt
     4921 gaaacttttt ctacgctgta gttgctaggt atgtcggata cccgaacagg ctcctcttcg
     4981 ctgacttcct cttcccgata cgctttcgta ggctcctcga tgatcggctc aggggtcgct
     5041 tcttcaatga ctgcctcctg ctccgcctgc atctgttcac gggaagcgaa ccactccttc
     5101 caggcatcct gcatgagcgc gactgcttca gccgaatcct cgacagcagg agccggttta
     5161 atggcctcca ggggcgggat agctggctca tcctccattg gagcgtatcg gggtgcctcc
     5221 caagcgctca cggggcttat cctctcctct acgtggctcg cttgaggcac atctgcgagc
     5281 gtttccttac cgaaacgatg ctcccggatc gaggattcaa gcacgtacca gttccggccc
     5341 acgagcttcg catccacgcg tccttctcgg cagagctggc ctacataatc cttggcgtat
     5401 ccggtgatct tggccgcttg ctttgacgag atgtagatct tgtcgccaat agtgagttca
     5461 tccatgctgt gagtataagc ttttctcgtc tcgtatgaaa tagcttttgc acagaccggt
     5521 agaaaagtga atggccccgt acctcggggc cattcgctac ttcccgagct cctcgatgat
     5581 gagctcccgc agcaccgttg ggttgccggc ccccttggtt gccttcatgc cctggccgac
     5641 gaggtactgg aggacgctct ccttgccgcc tcggtactcc tcggcgaccg cagggttcgc
     5701 agcgaggacc tcctgcactg ccttcctgag cgactccgta tcgctcacct ggagcagccc
     5761 tgcctcctcg acgaccagga gcgcggactc cccgcccttc tctcgcagca gcgcgagtgc
     5821 gtccttggcg ccgcgcgagg atatcttgtt ctccgcgatg agcgcgatga gctgcgcgaa
     5881 cgctttcggg gtgaggttat cgtacgcgcc ggcgacgtcg gtgatgagat agttcgcggc
     5941 gatcgaaatg cgatcaggtg caccaccgct ctccgcgagc accgcatcga agaatgcgct
     6001 gtcctcgatc gatcccgcga actgcgcggc atgctcgggc ttaaggccga gtccttcgta
     6061 ccgcgcccgc ttctccgccg gcagctcggg gagcgagctc tggatgtcgg caagccagga
     6121 gagttccgag agcacgagct tcgggaggtc gggttccggg aagtaccggt agtctgcgct
     6181 gccctcctta acccgctgac tgaacg
//
LOCUS       DASPKG010000045         4485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1297237, whole genome shotgun sequence.
ACCESSION   DASPKG010000045 DASPKG010000000
VERSION     DASPKG010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297237"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VEA36_01685"
     CDS             complement(<1..663)
                     /locus_tag="VEA36_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HYF29060.1"
                     /translation="MDTPRFYAADESALKARFSTGEHGLSTAEATKRLAAQGANALPE
                     ARPDGIGVIFLRQFASPLIYVLLGAAAIIFVTGHPVDAYIILGILVFNAIVGTIQEGK
                     AQDTLRALKSFATTSATVLRDGVEMLVPDTEVVAGDILILSEGGKVAADARIVQSRGL
                     RVDESSLTGESEPVEKSSALLADTDLTLGDQVNMAFKGTNVVAGSGRALVVATGTDTA
                     LGK"
     gene            complement(758..1336)
                     /locus_tag="VEA36_01690"
     CDS             complement(758..1336)
                     /locus_tag="VEA36_01690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYF29061.1"
                     /translation="MHLRRFLIPILLPTLLIVSIGAIHLYRTEVRTEVDRFIETVSFP
                     FKLARLLAEPAPPEILMPVEGARVRSVADTWGGARPDGRSHEGQDIFAARGTPIRPAA
                     RGYIVRVGENTLGGKVVFILGSGARGYYYAHLDGFGPAATIGSFVETEDVIGYVGNTG
                     NAERTSPHLHFGVYQDGEPVNPLPLLIDRSPP"
     gene            1432..1998
                     /locus_tag="VEA36_01695"
     CDS             1432..1998
                     /locus_tag="VEA36_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HYF29062.1"
                     /translation="MLESHKKKLVAFYERNRRMPGYKELMALTGFKSKNAVYKLIGRL
                     VEAGVVLKDREGHLSPGPRMRGAPLLGLVEAGFPSPAEEELLDVMDFDEYLTPHKESS
                     YILKVKGDSMIDAGICPGDLVIVERRPTYKPGQIVIASIEGEYTMKYLRKSGERYYLE
                     PANAAYKPIHPKGTLSIEAVVTAVVRKY"
     gene            2107..2577
                     /locus_tag="VEA36_01700"
     CDS             2107..2577
                     /locus_tag="VEA36_01700"
                     /inference="COORDINATES: protein motif:HMM:NF039500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HYF29063.1"
                     /translation="MDQEWNEDELLAFIVEAGRHTYADPDAKKLAAPIRPGCEEYLYE
                     QGDWKYHDSYAWSKDGGGEEIVYFKGTPVWVMNYYGYLIGDVDSKAIYGFLHEALQER
                     HPALPVRGAPLEREGFRYAIEFDRGDIGKFRGVERIYQEGALVYECSVHGGLVR"
     gene            2581..3912
                     /locus_tag="VEA36_01705"
     CDS             2581..3912
                     /locus_tag="VEA36_01705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HYF29064.1"
                     /translation="MPTEVGWGKHYYYPRAIVHVDGDGFFAACEVARDPTLRGKPVVT
                     GKERGIVSAATYEAKRRGITRGVPLKDVKRICPEAIIISSDYQLYTLYSLRMYEIMRR
                     YTPEVEEYGIDECFGDITGLRRVHHKSYEEIARAIKDDLHRELDITFSVGLSCTKVLA
                     KIGSNYRKPDGFTAIPLLGKDAFLSTVPVEKVWGIGANTGALLRKHGVQTAGEFANKN
                     EAWVRARVTKPILELWKELNGESVFPLTTEQKEDYHSIQKTRTFSPASDNPAFLYSEL
                     SKNIEGASRKARRYNLVATGLSFFLKSSEFQYRTHAVTLPRPTNVPSEILHALEPFFA
                     KVYRRGTRYRTTGITLTGLREAKPEQLDLFVPPERSEGMDRLFERVDELTEKYGRDTV
                     YLGSSAEAVAARRKAAEGDGIGSVSKYRPASLFEGKRRLKLFSLPVLGDVM"
     gene            3915..4433
                     /locus_tag="VEA36_01710"
     CDS             3915..4433
                     /locus_tag="VEA36_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012279476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HYF29065.1"
                     /translation="MEQLDLFPATVRAPHGFLYQPDFITIEEERELLRYLNGLKLKNY
                     EHGEYVAKRKVRWFSGAMPAPLALLRDRIALWSGKSPGEFTNALVSKYEPGAPIGWHR
                     DRPPYRAVFGVSLGSECRFRLRKREGQKWKRFTQVASARSVYLMSASARTHWQHSIPA
                     VEALRYSITFRI"
BASE COUNT          871 a         1391 c         1441 g          782 t
ORIGIN      
        1 cttcccgagg gcggtatcgg tgccggtcgc gaccacgagc gcgcggcccg accctgcgac
       61 cacgttcgtg cccttgaagg ccatgttcac ctggtcgccc aaggtgaggt ccgtgtccgc
      121 aaggagcgcg gatgatttct cgaccggttc cgattccccg gtcagcgacg actcatcgac
      181 gcggagcccc cggctctgga cgatgcgggc gtcggccgcg acctttcccc cctcggagag
      241 gatgaggata tcgcccgcga cgacctccgt atccggcacg agcatctcga cgccgtcgcg
      301 aagcacggtc gcgctcgtgg tagcgaacga cttcagggcc cgcagcgtat cctgcgcctt
      361 cccttcctgg atggtgccca cgatcgcatt gaagacgagg atgccgagga tgatgtacgc
      421 gtcgaccgga tgcccggtga cgaagatgat cgcagcggcc ccaagcagca cgtagatcaa
      481 ggggcttgcg aactgccgca ggaagatgac cccgataccg tccgggcgcg cctcggggag
      541 cgcgttggcg ccctgggcgg cgaggcgctt cgtggcctct gcagtcgaga gcccatgctc
      601 gcccgtactg aaccgcgcct tgagcgcgga ttcgtcggcc gcatagaaac gcggggtatc
      661 catggctcta ttatcgcgtc caaatacgtt gaatgctacg gaatgctcgg gagaacgtgc
      721 gtatagatgg aattggtact ccgaacggcg atactcctta gggtggcgag cgatcaataa
      781 ggagcggaag cggattgacc ggctccccgt cctggtacac cccgaaatgg aggtgcgggc
      841 tcgtacgctc cgcattcccc gtattcccca cgtacccgat gacgtcctcg gtctcgacga
      901 agctgccgat cgtggctgca gggccgaagc cgtcgagatg cgcgtagtaa tagccgcggg
      961 cgcccgagcc gaggatgaac acgaccttcc cgccgagcgt gttctctccg acccggacga
     1021 tgtagccgcg ggcggcgggc cgtatcggcg tgccgcgcgc agcgaagatg tcctggccct
     1081 cgtggctgcg tccgtcggga cgcgcgccgc cccaggtatc ggcgacgctc ctcacgcggg
     1141 cgccctcgac cggcatgagg atctcgggag gcgcgggctc ggccaaaagc cgcgcgagct
     1201 tgaacgggaa gctcacggtc tcgatgaacc gatccacttc ggtcctgacc tccgtgcggt
     1261 agagatggat cgcgccgatc gagacgatga ggagcgtcgg gaggaggatg ggtatgagga
     1321 agcgccgaag gtgcatagga accacgctac ggcctcagta cttaagcgcc cgtgaacctt
     1381 attcacacta tccccattga cggtgaacta tagttcacct atccttggag tatgctcgaa
     1441 agccataaaa agaagctcgt cgccttctac gaacggaacc gccggatgcc gggctacaag
     1501 gagctcatgg cgctgacggg cttcaaatcc aagaacgcgg tgtacaagct catcggccgg
     1561 ttggtcgagg cgggggtcgt cctgaaggac cgggaaggac atctctcgcc ggggccgcgc
     1621 atgcgcggag caccgctctt gggccttgtc gaggcagggt tcccctcgcc tgccgaagag
     1681 gagctgctcg acgtcatgga cttcgacgag tacctcacgc cgcacaagga atcgagctac
     1741 atcctcaagg tgaagggcga ctcgatgatc gatgccggga tctgcccggg agacctcgtc
     1801 atcgtggagc ggcggccgac ctataagcca gggcagatcg tgatcgcgtc gatcgagggc
     1861 gagtacacca tgaagtacct gcgcaagtcg ggcgagcggt actacctcga gcccgcgaac
     1921 gcggcataca agcccatcca tccgaagggg actctttcga tcgaagcggt cgtgacggcc
     1981 gtggtgcgca aatactaacc agctacctat gactacctct acccttccca gcctttacca
     2041 gctcgcgagt ttcgagcgcg tgctgcgccg gtcgccggtg cggattgcca gtaagcagcg
     2101 atcgctatgg accaggaatg gaacgaggac gaactcctag ccttcatcgt cgaggcgggc
     2161 cggcatacct atgccgaccc ggacgcgaag aagctcgctg cccccattcg tcctggctgc
     2221 gaggagtacc tctacgagca gggcgactgg aagtaccacg attcctatgc gtggagcaag
     2281 gatggcggtg gcgaagagat cgtctatttc aaaggaaccc cagtatgggt gatgaactat
     2341 tacgggtatc ttatcgggga tgtggattcg aaagccatct acggattcct gcacgaggcg
     2401 ctgcaggagc ggcatcccgc gctccccgtg cggggcgctc cgctcgagcg agaggggttt
     2461 cggtatgcga tagagttcga tcgaggcgac atcgggaagt tccgcggagt cgagcggatc
     2521 taccaggaag gagcactcgt gtacgaatgc tccgtgcacg gcgggctcgt gcgatgacct
     2581 atgccaacgg aggtcggctg gggtaagcac tactactatc cgcgtgcgat cgtgcacgtg
     2641 gacggcgatg ggttcttcgc ggcctgcgag gtcgcgcgcg acccaacgct gcgcggcaag
     2701 ccggtcgtca ccggcaagga acgcggcatc gtctcggccg cgacctacga agcgaagcgg
     2761 cgcggcatca cgcgcggcgt gcccttgaag gacgtgaagc ggatctgccc cgaggcgatc
     2821 attatctcat cggactacca gctctacacg ctctattcgc tccgtatgta cgagatcatg
     2881 cggcggtaca cgcccgaggt cgaggagtac ggcatcgacg aatgcttcgg ggatatcacg
     2941 gggctccgcc gcgtacacca taagtcgtac gaggagatcg cacgggcgat caaggacgat
     3001 ctccatcgtg agctcgatat cacgttctcg gtcgggctct catgcacgaa agtgcttgcg
     3061 aagatcggct cgaactatcg gaaacccgat ggcttcaccg ccataccgct cctcgggaag
     3121 gatgcgttcc tttcgacggt ccccgtcgag aaggtctggg gcatcggcgc gaacacgggc
     3181 gcgctcctcc ggaagcacgg ggtgcagacc gcgggcgaat tcgcgaacaa gaacgaggcc
     3241 tgggtgcgcg cgcgggtcac gaagcccatc ctcgagctct ggaaggaact caacggcgaa
     3301 tccgtgttcc cgctcacgac cgagcagaag gaggactacc attccataca gaagacccgg
     3361 acgttcagcc cagcgtccga taaccctgca tttctgtatt ccgaactctc gaagaacatc
     3421 gaaggcgcct ctaggaaggc gcgccggtac aacctggtcg cgaccggcct ttcgttcttc
     3481 ctcaagtcct ccgagttcca gtaccgcacg catgcggtca cgctcccccg gccgacgaac
     3541 gtaccgagtg agatcctcca tgcgctcgag ccgttcttcg cgaaggtgta tcggcgcggg
     3601 acccgctacc gcacgacggg catcacgctc acgggactgc gcgaggcgaa gcccgagcag
     3661 ctcgatctct tcgtcccgcc ggagcgaagc gaaggcatgg accggctttt cgaacgggtc
     3721 gatgagctca ccgagaagta cggccgggat acggtgtacc tcggttcgag cgccgaggcg
     3781 gtcgcggcac gaaggaaagc agccgagggc gacggcatcg ggagcgtctc gaagtaccgt
     3841 cctgcgtcct tgttcgaggg caagcggcgg ttgaagctgt tctcgcttcc ggtattggga
     3901 gacgtcatgt aagtatggag cagctcgacc tcttccccgc caccgtccgg gccccgcatg
     3961 gctttctcta ccagcccgac ttcatcacca tcgaagagga gcgggagctc ctgcggtacc
     4021 tcaacggctt gaagctcaag aattacgagc atggcgagta tgtcgccaag cgtaaggtcc
     4081 gctggttctc gggagcgatg ccggcgccgc ttgcgctgct ccgggatcgc atcgcgctct
     4141 ggtccggcaa atcgcccggg gaattcacga acgcgctcgt gagcaaatac gagcccggcg
     4201 ccccaatcgg ctggcaccgg gaccgaccgc cgtaccgcgc ggtcttcggc gtctcgctcg
     4261 gcagcgagtg ccgcttccgg ctccggaagc gcgaggggca gaaatggaag cggttcacgc
     4321 aggtcgcgag cgcccgctcc gtgtacctca tgtccgcgtc cgcgcgcacc cactggcagc
     4381 acagcatccc ggcggtcgag gcgctgcggt attccattac tttccggata tgaagcaatt
     4441 tttgaaatgg atggggctaa agaaacgtct gcatgattcc ctaca
//
LOCUS       DASPKG010000046         7777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1431754, whole genome shotgun sequence.
ACCESSION   DASPKG010000046 DASPKG010000000
VERSION     DASPKG010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431754"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VEA36_01715"
     CDS             <1..969
                     /locus_tag="VEA36_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HYF29066.1"
                     /translation="NPSRDVTFRDEVRGDITFHTGELGDFVIARAIDDPLYHFAVVVD
                     DWDMGITHVIRGDDHISNTTRQILIQEAVGAPRPIYAHLPLILDTDRSKMSKRSGNTS
                     LMAYRDEGFLPEGIVNYLALLGWSPGGNEEHFSLADLTRVFDLDGVQKSGAVWDREKL
                     LSVNQRWMRTLSDDEFLTRGGFDASVAKAVPLLKERARTFGEARELLAGELGCLFHAP
                     QLDRRTLMAKEPEDRPGFTKSALEALLPVISGLPEGVSADEAKAAVMPRADAEEAKGK
                     GGRGAVLWPLRYALSGQERSPDPFTLIALLGVHEAKSRLEAALGIL"
     gene            973..2322
                     /locus_tag="VEA36_01720"
     CDS             973..2322
                     /locus_tag="VEA36_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYF29067.1"
                     /translation="MASVRWHLYFLFFIFAALVLGSVSPMDSFAQSKEEIEERIEQHN
                     DAIKKLEEEIAIYQKELNTLGVQKQTLQSAIKTIDVSRQQTGTQIRVTENRIAATNLK
                     LQELSGEISRKEYLIQLDKQTLAQSIRDIDTEGDNSLIEQLFSSVSLADAWEAMDTKA
                     MLGQALRAHASALARDKQELTVKQIEEDGKKRELASHSTTLKNEKRTLDVQKAEKDRL
                     LAQTKNQESTYQSIIAQKQAQRKAFEAELSALENSLKSVGAAAVPAVGSGILAWPYSA
                     AFKESCNGKAGALGNAYCITQYFGNTAFATANAQIYNGNGHNAIDIGMPVGTPVLSAL
                     SGTVLATGNTDAYPGCYSFGKWVMVKHANGLSTLYSHLSSIAVAKGQAVSTGELVGHS
                     GMTGYATGPHLHFGVYASAGVQIMSLGQFRNTGGSGCTNAVMPVAPKDAYLNPMSYL"
     gene            2323..2814
                     /locus_tag="VEA36_01725"
     CDS             2323..2814
                     /locus_tag="VEA36_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HYF29068.1"
                     /translation="MNEAIPEPAPSGKIELRASTLDGAGRGVFATVPLADGELVESCP
                     VIALPDKRDRARLRKTGLVNYYFLWGKERKHAAICLGFGSLYNHSYEPNCFYVKRIEE
                     AQMDFYALRDIQAGEELTVNYNGVPTDTRTLWIRSIPPANAVASERSSRSFIERVRRL
                     LER"
     gene            complement(2811..3467)
                     /locus_tag="VEA36_01730"
     CDS             complement(2811..3467)
                     /locus_tag="VEA36_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HYF29069.1"
                     /translation="MKPKRTMKGMAPMRHFKAADPVLYRAALPHHRHISLEHRRGYRK
                     NDLFAKLVGSIVSQQLSTKAADTIWGRLKDACGGAVTPEAIKKLRTPTMRKAGLSAAK
                     VKTLKELSAAILSGRLDLAALKKLPEEEAIAKLSAVWGIGRWTAEMFLMFALDREDVF
                     SLGDLGLIRAMEELYGIPKNSKPQVYEAVAIKWSPHRTFACRVLWRLRDAPNQDSAVD
                     "
     gene            3490..3705
                     /locus_tag="VEA36_01735"
     CDS             3490..3705
                     /locus_tag="VEA36_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29070.1"
                     /translation="MIRTIILILAIVLVLSFFGISIQSVVQSPAGQENFGFLWSLIKE
                     GWEVVKDGWDAFKEFLGNLFRPIQFPR"
     gene            3710..3967
                     /locus_tag="VEA36_01740"
     CDS             3710..3967
                     /locus_tag="VEA36_01740"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HYF29071.1"
                     /translation="MDTPDADPITITLPVDSGPSFANATQVMVSDDSVILQFAYVRPK
                     SRTGQLLCELALSPKHAIEFSRALDATIKKHFTRHLGDING"
     gene            4080..4316
                     /locus_tag="VEA36_01745"
     CDS             4080..4316
                     /locus_tag="VEA36_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29072.1"
                     /translation="MKMLFLGALALAAAVVVPGVSFAQTFAYVNQDGEVMTYEAATAM
                     QALVAAPAIDEHSGVLLVDGADDQQLVGDEVPGV"
     gene            4454..5272
                     /locus_tag="VEA36_01750"
     CDS             4454..5272
                     /locus_tag="VEA36_01750"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYF29073.1"
                     /translation="MSSFTTFIGIALVAMLVALPAGASAASASASFADDTITARSTRP
                     SITGEAEGTKSVRVVVRSEAGKIVYKKDARVRDGEWTVRISKRLKAGEYDLAVYAGKS
                     TRRDALDEGTLTIEGKKSTKKEKASKSGSSDGILTVSSVPLLTGGTARLNASVPVAYV
                     KITNQGTEEVALEGMTLQQTGTAAVSAISTFSTSDDKGGSRATVNAAFKGKSAFVPLA
                     ATVAPGQMRIFTVKAGVAPTAPLGSTLKLDVTDVTTDARVYGAPLRGTTWTIGL"
     gene            5693..>7777
                     /locus_tag="VEA36_01755"
     CDS             5693..>7777
                     /locus_tag="VEA36_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HYF29074.1"
                     /translation="MADLQLLERVGKELARGAPHDEIVAALEKEGWSAGDVRGAIAIR
                     SLEQIGKQQGNRKLISELSPLVAPRQRYASDGVPAGPFLVLVAILLVVAGGALYLYEP
                     SSLDFITRGRTAAAVPTEFGGTMLAETSRTYTHTYTFTEEPKSAAPKPASSGGSSFVP
                     PLTTYVPPSPAPSVLSAIEPVEETGTAEGAVTAPTARPTVRLTLAETSIAPNTSTTLT
                     WSAKNATSCSSTGFDIAGQQTGKVTVTTSDRTRFTLTCTGPGGERTTSAFLSVNIIVP
                     PPPEQVAAAPTPTPDPVPVAIPEPEVTPPATPSGPPQEIPEIAQNPDGYPVYPGCEQP
                     ATTYARELYIDPVGGNDSGDGSRTAPYRSFSAVIAAKKIQPGDHVVLLAGNHGPISLK
                     GAGLPAFASATSWTWLDFQSGATGYSVSAEGISRLLVTGGEFVKDPAATSVGGLRFWI
                     TRSSHIVVADTRQYHAKDSAGWDVPKWMSLSSGMQANNSTCTSFVNNRIKNVRGGIGV
                     IKGGNTPAENSAKALVKGNEIRNFSADGMVMNGSDITVRDNRLYDQYAGMEDGDPNHD
                     DGLQMFAGVNQVFDNVRIEDNWFQERTSLNRELDAHMQGIVVFDGKVTNMRVTGNVVL
                     GSAYHGIALYGAQNSLVDHNTVQNPTTNGHAYWISVPKAKAAFGAFPPTGTKVSNNIA
                     HAYIIAEGATASN"
BASE COUNT         1439 a         2492 c         2484 g         1362 t
ORIGIN      
        1 aatccgtcgc gcgacgtgac cttccgggac gaggtccgcg gcgacattac cttccatacg
       61 ggcgagctcg gtgacttcgt gatcgcccgc gccatcgacg accccctgta ccacttcgcg
      121 gtcgtggtgg acgactggga catgggcatc acgcatgtca tccgcggcga tgaccatatt
      181 tccaatacga ctcggcagat cctcatccaa gaggccgtgg gcgcgccacg tccgatctat
      241 gcgcatcttc cgcttatcct cgatacggac cgctcgaaga tgtcgaagcg aagcggaaat
      301 acgtccctca tggcgtaccg ggacgagggc ttcctccccg agggcatcgt gaactacctc
      361 gcgctgcttg gctggagccc gggcggcaac gaggagcatt tctcgcttgc ggacctgacg
      421 cgggtattcg atctcgacgg cgtgcagaag tccggtgcgg tatgggaccg ggagaagctg
      481 ctctcggtga accagcgatg gatgcggacg ctgtctgacg atgaattcct tacccgaggc
      541 ggattcgatg cgtcagtagc caaggcggta ccgctcctga aggagcgcgc ccgcaccttc
      601 ggggaggcga gggagcttct cgcaggggag cttggctgcc tctttcatgc gccgcagctc
      661 gaccggagaa ccctcatggc caaggaaccc gaagaccgcc caggattcac caagtcggcg
      721 ctggaggccc tgctgccggt catatcaggg cttccggagg gcgtctctgc cgacgaagcg
      781 aaggcggccg tcatgccccg tgcggacgcc gaggaagcga aggggaaggg aggccggggc
      841 gcggtgctct ggccgctccg gtacgcgctc tcggggcagg agcggtcgcc ggatccgttc
      901 accctcatcg cgctcttagg cgttcatgaa gcgaaatcgc gcctcgaagc ggcgcttggt
      961 atactgtagg taatggcatc ggttcggtgg cacctctact ttctcttttt catattcgcc
     1021 gccctcgtgc tcgggtccgt ctcgccgatg gactccttcg cgcagagcaa ggaggagatc
     1081 gaggagcgga tcgagcagca taacgatgcg atcaagaagc tcgaggagga gatcgccatc
     1141 taccagaagg agctgaacac gctcggggtg cagaagcaga ccctgcagtc cgcgatcaag
     1201 acgatcgatg tctcgcggca gcagacgggg acccagatcc gcgtcacgga gaaccgcatc
     1261 gcggccacga acctcaaatt gcaggagctc tcgggggaga tcagccggaa ggagtaccta
     1321 atccagctcg ataagcagac cctggcccag tccatacggg acatcgatac cgaaggcgac
     1381 aactcgctca tcgagcagct cttctcctcg gtatcgcttg ccgacgcctg ggaagcgatg
     1441 gataccaagg cgatgctcgg gcaggcgctg cgcgcccacg cgagcgcgct ggcccgcgac
     1501 aagcaggaat tgaccgtcaa gcagatcgag gaggacggga agaagcgcga gctcgccagc
     1561 cacagcacga ccctcaagaa cgagaagcgc acgctcgacg tccagaaggc agagaaggat
     1621 cggctgctgg cgcagacgaa gaaccaggag tcgacctatc agagcatcat cgcgcagaag
     1681 caggcgcagc ggaaagcctt cgaggccgag ctctcggcgc tcgagaacag cctcaagtcc
     1741 gtgggcgccg cggcggtccc tgccgtgggc tccggcatcc tcgcctggcc gtattcggcg
     1801 gccttcaagg agtcctgcaa cggcaaggcg ggcgccctcg ggaacgccta ctgcattacg
     1861 cagtacttcg ggaatacggc gttcgcgacc gctaacgcgc agatctataa cggcaacggc
     1921 cataacgcga tcgacatcgg catgccggtc gggacccccg tcctctcagc gctctccggg
     1981 accgtgcttg cgaccgggaa cacggacgcg tacccgggct gctattcctt cggtaagtgg
     2041 gtgatggtca aacacgccaa cgggctctcc accctctatt cgcacctttc ctcgatcgcg
     2101 gtcgccaagg gccaggcagt gtcgaccggg gagctcgtcg gtcactccgg catgacgggg
     2161 tacgccaccg gcccccatct tcacttcggg gtgtatgcat ctgccggcgt gcagatcatg
     2221 agcctcgggc agttccggaa caccggcggc tcgggctgca cgaacgcggt gatgccggtg
     2281 gcgccgaagg acgcgtactt gaacccgatg tcctacctgt agatgaacga agcgatcccg
     2341 gaaccggctc cctcggggaa gatcgagctt cgcgcctcga ccttggacgg cgccggccgc
     2401 ggggtcttcg ccaccgtacc gcttgccgac ggggagctcg tcgagagctg cccggtcatt
     2461 gcgctgccgg acaagcggga ccgagcgcgg ctccggaaga ccgggctcgt aaactactac
     2521 ttcctctggg ggaaggagcg caagcacgcc gcgatctgcc tcgggttcgg gtccctctat
     2581 aaccattcct acgagccgaa ctgtttttat gtaaagcgca tcgaggaggc gcagatggat
     2641 ttctatgcgc tccgggatat tcaggcggga gaggagctga ccgtgaacta caatggagtg
     2701 ccgaccgata cgaggaccct ctggatccgg tccatacccc cagcgaacgc ggtggcgagc
     2761 gagcgatcca gccggtcgtt tattgagcga gtccggcggt tgctcgagcg ctagtccacc
     2821 gcgctgtcct ggttcggtgc atcccgcagc cgccagagca cacggcacgc gaaggtgcgg
     2881 tgcggcgacc attttatagc gacggcctcg tacacctggg gcttcgagtt cttcgggatg
     2941 ccgtagagct cctccatggc ccggatcagg ccgaggtccc cgagcgagaa gacgtcctcg
     3001 cggtcaagcg cgaacataag gaacatctcg gcggtccagc ggccgatgcc ccataccgca
     3061 gagagcttcg cgatagcttc ctcctcggga agcttcttga gggcagcgag atcgaggcgg
     3121 ccggagagga tggcggcgga cagctccttc agggtcttca ccttagcggc cgataggccc
     3181 gccttgcgca tggtaggagt gcggagcttc ttaatggcct ccggcgtgac cgcgcctccg
     3241 caggcgtcct tgaggcgccc ccagatggtg tccgcggcct tggtcgagag ctgctgcgag
     3301 acgatggacc cgacgagctt cgcgaagagg tcgttcttcc ggtacccccg gcggtgctcg
     3361 agcgatatat gccgatggtg gggcagcgcc gcccggtaca ggaccgggtc cgccgccttg
     3421 aaatgccgca tgggcgccat ccccttcatc gtccgtttgg gcttcactgg cctagtatac
     3481 tgaaggcaca tgatccggac catcatcctc atcctcgcca tcgtgctggt gctttccttc
     3541 ttcggcatct cgatccagag cgtcgtgcag tctcctgcag gccaggagaa tttcggcttc
     3601 ctctggagcc tcatcaagga ggggtgggag gtcgtgaagg acgggtggga tgcgttcaag
     3661 gaattcctcg gtaacctctt tcggccgatc caattcccgc gctagcccta tggatacccc
     3721 cgacgcagat cccatcacca tcacgctccc cgtcgatagc ggcccctcgt tcgcgaatgc
     3781 gacccaggtc atggtctcgg acgattccgt catcctccag tttgcgtacg tgcggccgaa
     3841 gtcccggacc gggcagctcc tgtgcgagct cgccctctcc ccgaagcacg cgatcgagtt
     3901 ctcgcgggcc ttggacgcca ccatcaagaa gcatttcacc aggcatctcg gggatatcaa
     3961 tggttgagcc atacctttac ttcatccggc attcatgtcc tattcacggg ccgttcatgg
     4021 cccgcggtat ataccgtaga ggttaggcga ttacgcccta acgctaaccg tacgattgta
     4081 tgaaaatgct attccttggc gcgctcgcgc tcgccgcagc ggtcgtggtg ccgggcgtct
     4141 ccttcgccca gaccttcgcc tacgtgaacc aggacggcga ggtgatgacc tacgaggccg
     4201 ccaccgcgat gcaggcgctc gtcgcggctc ctgcgatcga tgagcacagc ggcgtgctcc
     4261 tcgtcgacgg tgcggacgac cagcagctcg tcggcgacga ggtgcccggc gtgtaagcgc
     4321 aaggcgcgcg caacgtctga actcccgaca cccttcggga gttcttcgtt tgcttcatga
     4381 agcccgctcc tacggccgcg ctcgcatgaa gctgcgtcgc tcgctatagt gggagtctct
     4441 aacacgcatg tctatgtcat cattcactac attcatcggt atcgcgctcg tcgcgatgct
     4501 ggtcgcgctc ccggcgggcg catccgccgc ttccgcgagc gcgtcgtttg cggacgatac
     4561 catcaccgca cgctcgaccc ggccgagcat cacgggcgag gctgaaggca cgaagtcggt
     4621 ccgcgtcgtc gtgcgctccg aggcggggaa gatcgtctat aagaaggacg ctcgggtgcg
     4681 ggatggggag tggaccgtcc gcatctcgaa gcggctcaag gccggcgagt acgatctcgc
     4741 ggtctacgca gggaagagca cccgccgcga cgcgctcgat gaaggcacct tgaccataga
     4801 gggcaagaag agcacgaaga aggagaaggc gtcgaagagc ggttcgtctg acggcatcct
     4861 caccgtctcc tccgttccgc tcctgacggg cggcaccgcg cgtctcaacg cgtccgtccc
     4921 cgtcgcgtac gtgaagatca cgaaccaggg cacggaggag gtcgctctcg agggcatgac
     4981 gctccagcag accggcaccg cggccgtctc cgcgatctcg accttctcga cgagcgacga
     5041 taagggcggc tcgcgcgcaa cggtcaacgc cgccttcaaa gggaagtctg cgttcgtccc
     5101 gcttgcggcg acggttgcgc ctggccaaat gcgcatcttt acggtgaagg ctggcgtcgc
     5161 cccgaccgcg cccttgggct cgaccctgaa gctcgacgtc acggacgtta cgaccgatgc
     5221 gcgcgtctac ggcgccccgc ttcgcggcac gacctggacc atcgggctct agcagaggag
     5281 ctatcgtgcg taacgcaaaa gccgcccgag ggcggcttta tgcgtaaacg cgtgttcagt
     5341 ccgggacgag gataccgtgg gtccccgtgg cgcaggcgcg tagccgttgc ctttaccgga
     5401 gcgtttctga cggatcggat tttcctgcgt tcgccgtctt ccggggaagc cggcggcgca
     5461 catccgtcac gctcgaggtt cactggactg cccgcacgtt tcgtttccgg atgcccgggt
     5521 gccgcgcgag cggtccttgg cttccgagcc ccgatactga ttgctccgta tcggatagtt
     5581 gtagggtacc gagaggggga taaccgggca atggtggata ctgggcatat gctggggaga
     5641 aaaatgggga taacgaatcg ggggaggggg gctggtatag tttttgtatt ctatggctga
     5701 cctccagctg ctcgagcgcg tcgggaagga gcttgcgcgc ggagctccgc acgatgagat
     5761 cgtcgctgcg ctcgagaagg agggttggag cgcaggcgat gtccgcggcg ccatcgccat
     5821 ccgctcgctc gagcagatcg gcaagcaaca agggaaccgc aagctcatct cggagctctc
     5881 gccgctcgtg gcgcccagac agaggtatgc gtcggacggg gtgccggccg gccccttcct
     5941 tgtcctcgtc gcgatcctcc tcgtagtcgc gggcggtgcc ctctacttgt acgagccatc
     6001 gagccttgac ttcatcacgc gcgggaggac cgcggcggcc gtgccgaccg agttcggtgg
     6061 gacgatgctc gccgagacgt cgcggaccta cacccatacc tatacgttca cggaggagcc
     6121 caagtctgct gcgccgaagc ccgcgtcaag cgggggttcg tccttcgtgc ctcctttaac
     6181 gacctacgtc ccgccctccc cagccccgtc cgtcctctcg gcgatcgagc cagtcgagga
     6241 gacgggaacg gccgagggtg ccgtgacggc gccgaccgcg cgccccaccg tgcggctcac
     6301 gcttgcggag acctcgatcg cgccgaacac ctccacgacg cttacctgga gtgcgaagaa
     6361 cgcgacctcc tgcagctcga cgggcttcga tatcgcgggg cagcagacgg ggaaggtaac
     6421 cgtcacgacg agcgaccgca cgcgcttcac cctcacctgc acgggcccgg gaggcgagcg
     6481 gaccacctcc gcattccttt ccgtgaacat catcgtgccg ccgccgcccg agcaggtggc
     6541 tgccgcaccg acgccgaccc ctgatccggt accggtcgct atcccggagc cggaagtgac
     6601 gccgcccgca acgccttcag ggcccccgca ggagattccg gagatcgcgc agaacccgga
     6661 cggctacccg gtatacccgg gctgcgagca gccggcgacg acctatgcgc gcgaactgta
     6721 catcgatccg gtgggtggga atgactcggg cgatggctcg cgtaccgcac cctaccgctc
     6781 gttttcggcg gtgatcgccg caaagaagat ccagccgggc gatcacgtgg tcctcctcgc
     6841 cggcaatcat ggaccgattt ccttgaaggg agctggcctt ccagcattcg cgagcgcaac
     6901 gagctggacc tggctcgatt tccaatccgg cgcgaccggc tacagtgttt cggccgaggg
     6961 catatcgcgg ctgctggtca cgggcggtga atttgtgaag gatcctgccg ccaccagcgt
     7021 cggtggcctg cgcttctgga tcaccaggag ttcccacatc gtggtcgccg atacccgcca
     7081 gtatcacgca aaggattccg ctggatggga cgtgccgaag tggatgagtc tctcgagcgg
     7141 catgcaggcg aataattcca cctgcacctc gttcgtgaac aataggatca agaatgtgcg
     7201 cggcggcatc ggtgtgatca agggcggtaa tacgcctgcg gagaatagcg ccaaggcgct
     7261 cgtcaagggc aacgagatcc gcaacttctc agccgacggc atggtcatga acggatcgga
     7321 catcacggtc cgcgataaca ggctctacga ccagtacgcg ggcatggagg atggcgatcc
     7381 gaatcacgac gatgggctcc agatgttcgc gggcgtgaac caagtcttcg ataacgtccg
     7441 catcgaggac aactggttcc aggagcgcac gagcctcaac cgcgagctcg atgcgcacat
     7501 gcagggcatc gtggtattcg acgggaaggt cacgaacatg cgcgtgaccg ggaacgtcgt
     7561 gctcggcagc gcctaccacg gcatcgcgct ctacggcgcg cagaacagcc tcgtggacca
     7621 caacacggtc cagaacccga ccacgaacgg ccatgcgtac tggatctcgg tcccaaaggc
     7681 caaggccgcc ttcggcgcct tcccgccgac gggtaccaag gtctctaaca acattgcgca
     7741 cgcgtacatc atcgctgagg gcgcgacggc ctcgaac
//
LOCUS       DASPKG010000047         9982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1705723, whole genome shotgun sequence.
ACCESSION   DASPKG010000047 DASPKG010000000
VERSION     DASPKG010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9982)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9982
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705723"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VEA36_01760"
     CDS             complement(<1..256)
                     /locus_tag="VEA36_01760"
                     /inference="COORDINATES: protein motif:HMM:NF019187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29075.1"
                     /translation="MFVVRHRTFFFWLTGLLLVGSIVALIVIPPRLSIEFTGGTLVEV
                     AYTGERPPHEELVARVEALDLGEPSVRESGDRGVTVRSHTL"
     gene            complement(258..1574)
                     /gene="secD"
                     /locus_tag="VEA36_01765"
     CDS             complement(258..1574)
                     /gene="secD"
                     /locus_tag="VEA36_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HYF29076.1"
                     /translation="MRYLPVAGVLLVTALLAWFVVSTSGINSQFPIKLGLDLAGGTEL
                     IYRADTSNIVSDKQGALNSLRDVIENRVNLFGVAEPRVELEQSSAVAGEVEDRLLIEL
                     PGVTDIDAAVAAIGETPVLEFKLASADVTDTATSTGTFTDTGLTGRYLKSSELQFGQG
                     GASGVPNQPIIVLNFNDEGAALFEQLTRENVGAPLAIFLDGELISAPIIREAIPGGVA
                     TITGQFTPTEARDLVRNLNFGALPVPIELVGSNTVGPTLGADAYSAGLYATVIGFGLV
                     ALFMILWYRLPGLIATIALIIYMLITIALIKVVPITLTASGIAALILSIGMAVDANVL
                     IFERMKEELRLGKSPREALHIGFGRAWPAIRDGHLTMLISSVILFWMGTSIVQGFALV
                     FGLGVIASLITAVMITRVFLVAILPEQGSRTWRALLEAGFGSRNST"
     gene            complement(1594..2412)
                     /locus_tag="VEA36_01770"
     CDS             complement(1594..2412)
                     /locus_tag="VEA36_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HYF29077.1"
                     /translation="MKIDGRAIAAAMLAEVASEVKFIGRPLTLRAVVSEPSPATESYL
                     RIKSDRAKDAGITLEIATIRDGAATEDLVRAVAAPGADGVIVQLPVPAGVDSEEVLNA
                     IPLALDADVLSATAYERFARGEPGALLPPVVGAVAEILHRAGAAVAGKRAVVVGSGRL
                     VGQPAALWLAAQGAHVATITRETGDLSMLKEADIVVAGAGSAHLIRPEHLAPGVVLID
                     AGTSESGGTLAGDADPACADIASVFTPVPGGVGPVAVAYLMKNVAELARLRTSA"
     gene            complement(2409..3311)
                     /locus_tag="VEA36_01775"
     CDS             complement(2409..3311)
                     /locus_tag="VEA36_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYF29078.1"
                     /translation="MKKAVVTGGAGFIGAHIARALIDVGYETHVVDDLSDGKRDRVPE
                     GATLHVLDVRNTSGIKEIFEGAAAVFHEAALPRVPFSIDHPQEAHDVNVNGTLSVLLA
                     AKEAGVSRVIYAASSSAYGEQATLPLSEDMPALPVNPYGLHKYIGECYARMFSELYGL
                     ETVSLRYFNVYGSGMDPEGAYALAIPKLLQLRAKGVPLTITGDGTQTRDFTHIRDVIR
                     ANLLAMESPRVGKGEVINIGSGRNVSMNYLAELIGGERVYIPARVEAHDTLADITRAK
                     ELLDWEPEVTLEDGIMGIRGEYGV"
     gene            complement(3308..4375)
                     /locus_tag="VEA36_01780"
     CDS             complement(3308..4375)
                     /locus_tag="VEA36_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYF29079.1"
                     /translation="MKHTLLITGAAGYVGTMLVQEFARRDDVERVIGLDREPVPDLIK
                     DEPKLVYIQASTEDGSWQGQVAAYRPDLVIHTAWDIRAVYGNTEATWRSNIDGSDAVF
                     DFAFENVFVKRLVHFSTVASYGSFPDNTIEYRFTEEDPFRKTDYLYAEEKRIAEVHLR
                     ERYEASDKKTEVAIVRPAAITGPRGRFMRMRFGLQAALSGQLRGARIYAVIKALVAFV
                     PVTPKWARQFIHEDDVVGIVERLAFGDSAGAYEVFNICPPGPIVRGEDMARAVGKRAL
                     PVFPWMVRIAFFVFWHLSRGKVPTTRGSWKGYSYPIVVDGGKVTRQLGYTYQHEGYDA
                     FFYTNGRYEPYVPEEARRHKV"
     gene            complement(4385..5749)
                     /locus_tag="VEA36_01785"
     CDS             complement(4385..5749)
                     /locus_tag="VEA36_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HYF29080.1"
                     /translation="MTHGGIRASALLFIGDLATFVAALWLTLLLRYGTLPTEAVFRDH
                     MGPFSLLFALWVLVFYMSGLYSKRFILFKSSLPNAILRTQVINIVMAALFFFLVPGVG
                     IAPKTNLVIYLLVSLALIFAWRLALYPRLSAPRVRDYAVLIAEGEETDELVREVNGNR
                     RYHLEFRKVYTPAALHALGREGLRRELGDKSIDLIVADTDCAEVQAMLPDLYMLPCFE
                     HGCVYADFDAVYEEVFDRIPLSLLSQGWFLENVTTTTPVFHAFVKRVIDLVGGVLMGL
                     VTMVAAPVVWAANLLEGGGPLFTVQDRIGIRGSSIRTYKFRSMRFTDTGMWPGEGENR
                     VTRVGDFLRMTSLDEFPQFVNVLTGELSLIGPRNDIRALGDRLAEALPYYNMRYIVTP
                     GITGWAQINQQYEQGHISPQSIEETKMRLAYDFYYIKHRSLALDIVIALKTFKRMFFR
                     LSNW"
     gene            5785..6906
                     /locus_tag="VEA36_01790"
     CDS             5785..6906
                     /locus_tag="VEA36_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYF29081.1"
                     /translation="MEVAPKRILYVITLGWWGGAQRYVFDLATAAKSAGHDVFVLTGE
                     GELRTRLEEVGVTAFSATHFQRGLGLRAELGAITSLGHAIRAVRPDIVHLNSSKAGFL
                     GAIVARFCGVKRIIFTAHGWAFSEVRPDWQRAIFWFAHYLTLLLVDRVICVSQAVRRG
                     TRFMPLVQGKLTVIYNGVPDIAFLTRKDAREQLAPDLPYPFWIGTIAELHPNKRVDVL
                     IRAFAAIMDECPDAAVVIMGEGTEREHLEALVARLGLEDRIRLCGHTPKAATYLTALD
                     LFILPSDTEALGYVLIEAGFAGLPVVASKVGGTPEIIESGIDGFLVQRRKVRAFAEAI
                     RQLHDDPALRARFGRALKEKVAARFSQQQMVDKTLILYR"
     gene            complement(6903..8039)
                     /gene="rodA"
                     /locus_tag="VEA36_01795"
     CDS             complement(6903..8039)
                     /gene="rodA"
                     /locus_tag="VEA36_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HYF29082.1"
                     /translation="MSEQVRRNPLAALVSEWPLLLIPLALCLLGVLTMHPFGEEASLA
                     PRQLIWIAGATLVFLVCSALDMRFIRRTGVLVSAYVVVLGLLALLLVATHAVQGAKSW
                     FDLGAFSFQPSDPAKIVFVALLAKYFSRRHMEIGNFRHVLVSALYAGIPILLILVQPD
                     LGTAAILGVVWFGMILVSGISKKHLALILAAGVIGAGMLWAFALEEYQRERIVSFLNP
                     AADIRGSGYNAYQATIAVGSGELMGKGIGYGTQSKLRFLPEYETDFIFAAFAEEWGFI
                     GITLVLILYALLLLRLVAIAQRAATNFDAFFTIGVTLVLFAHIFIHVGINLGLLPVTG
                     TTIPFMSYGGSHLLMEFAGLGIVASLARHGRAAPRDAAMQEYEG"
     gene            complement(8036..8791)
                     /locus_tag="VEA36_01800"
     CDS             complement(8036..8791)
                     /locus_tag="VEA36_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29083.1"
                     /translation="MSWASRRRFIVISILAAIAIALLAAIVIPIVYETPTCSDKKRNQ
                     DETGVDCGGSCARVCEAEALSPKVEFARIVSPRAGRTDVIAYVANPNKEAAVRSARYT
                     VELYGSAGSIVATRQGTMDLPPGATVPVFVPGLPADEAGTRAFFVFDPASLVFVTYQD
                     RRIVPRYNNDAQVMGTDGPRIMASFSNPSATALRNIPVIATVFDADGNAIAATQTLLA
                     ELPPQGSAQVVFVWNAPFSAAPARIDVVPIVPL"
     gene            complement(8971..9077)
                     /gene="rrf"
                     /locus_tag="VEA36_01805"
     rRNA            complement(8971..9077)
                     /gene="rrf"
                     /locus_tag="VEA36_01805"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(9159..>9982)
                     /locus_tag="VEA36_01810"
     rRNA            complement(9159..>9982)
                     /locus_tag="VEA36_01810"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1995 a         3156 c         3080 g         1751 t
ORIGIN      
        1 caagcgtgtg gctgcggacc gtgacgcccc ggtccccgct ctcgcggacc gagggctcgc
       61 cgaggtcgag tgcctcgacg cgcgcgacaa gctcctcgtg cggcggccgc tcgcccgtgt
      121 acgcgacctc gaccagggta ccgcccgtga attctatgga aagccggggc gggatgacga
      181 tgagtgcgac gatggacccc acgaggagga gccccgtgag ccagaagaag aaggtgcggt
      241 ggcggacgac aaacatacta ggttgagtta cggctaccga acccggcctc aagcaaggca
      301 cgccaggtac gcgatccctg ctcagggagg atggcgacga ggaagactcg cgtaatcatg
      361 acggcggtga tcaaggacgc gatcacgccg agcccgaaca cgagcgcgaa gccctgcacg
      421 attgaggtgc ccatccagaa gaggatgacg ctcgagatga gcatggtcag gtggccgtca
      481 cggatcgcag gccaggcgcg cccgaacccg atatggaggg cctcccgggg gctcttcccg
      541 agcctgagct cctccttcat gcgctcgaag atgagcacgt tggcatccac tgccatgcca
      601 atcgagagga tgagcgcggc gatgccggag gcagtcagcg tgatcggcac caccttgatg
      661 agcgcgatcg tgatgagcat gtaaatgatg agtgcgatcg tcgcaatcag gcccgggagc
      721 cggtaccaca agatcatgaa gagcgcgacg agcccgaacc cgatcacggt cgcgtagagg
      781 cccgcgctgt aggcgtcggc gcccagggtc gggcccacgg tgttgcttcc cacgagctca
      841 atgggaaccg gcagcgcgcc gaagttcaaa ttgcggacca ggtcgcgcgc ctcggtcggc
      901 gtaaactgac cagtgatggt cgcaacgccg cccgggatgg cctcgcgaat aatgggagcg
      961 gagatgagct cgccgtcgag gaagatcgcg agcggcgcgc cgacgttctc gcgcgtgagc
     1021 tgctcgaaga gcgctgcgcc ctcatcattg aaattgagca cgatgatcgg ttggttgggc
     1081 accccgcttg cgcccccctg cccgaactgg agctcggagc tcttcaagta ccgacccgta
     1141 agcccggtat ccgtgaaagt gccggtcgag gtggcggtat cggtcacatc cgccgaggcg
     1201 agcttgaact cgagcaccgg ggtctccccg atcgcggcga cggcagcgtc gatatcggtg
     1261 acgcccggca gttcgatgag gaggcggtcc tcgacctcgc ccgcgaccgc gctcgactgc
     1321 tcaagctcga cgcggggttc cgcgaccccg aagaggttca cccggttctc gatgacgtcc
     1381 cggagcgaat tcagggcccc ctgcttatcg ctcacgatat tgctcgtgtc cgcccgatag
     1441 atgagctcgg tgccgccggc aaggtcgagc ccgagcttga tcgggaattg gctgttgatc
     1501 cccgaggtcg agacaacgaa ccaggcgagc agcgccgtga cgaggaggac gcccgcaacc
     1561 gggagataac gcatgatgcc gccagtatac gggctaggcg ctcgtgcgta aacgcgcgag
     1621 ttccgccaca tttttcatga gataggcaac cgcgaccggc cccacgccgc ctggcaccgg
     1681 cgtgaagacc gaggcgatat cggcgcaggc gggatcggca tcgccggcga gcgtgccgcc
     1741 ggattccgag gtcccggcgt cgatgagtac gaccccaggg gcaagatgct ctggccggat
     1801 gaggtgcgcg cttcctgcgc cagctactac gatatcagcc tccttgagca tggagagatc
     1861 gccggtttcg cgcgtgatcg tggcgacgtg agcgccctga gcagcgagcc agagcgcagc
     1921 aggctgcccg acgaggcgac cgctgcccac gaccacggcc cgcttgccgg cgacggcggc
     1981 gccggcgcga tgcagtatct cggcgaccgc acccacaacg ggcggcagca gcgcgcccgg
     2041 ctccccgcgc gcaaaccgct cgtacgcggt cgccgagaga acgtccgcat cgagcgcaag
     2101 cggtatggca ttcagtacct cctcgctgtc gacgcccgcc ggcaccggca gctgcacgat
     2161 gacgccgtca gcgcccggtg ccgcgaccgc ccggacgaga tcctcggttg ccgcaccgtc
     2221 cctgatcgta gcgatctcaa gggtaatccc cgcgtctttg gcgcggtcgc tcttgatccg
     2281 gaggtaggac tcggttgccg gcgaaggttc ggacacgacc gcacggagcg tgagcggacg
     2341 tccgataaac ttgacctcgc tcgcgacctc ggcaagcatc gcagcggcaa tggcgcggcc
     2401 gtcgatcttc atactccgta ctcgccccgg atccccatga tcccgtcctc aagcgtaacc
     2461 tcgggctccc agtcgagaag ttccttcgcc cgcgtaatat ccgcgagcgt atcatgcgct
     2521 tcaacgcgag cgggaatata tacccgttct ccgccgatga gctccgccag gtagttcatt
     2581 gaaacattgc ggccgctgcc gatattaatg acctcaccct tcccgaccct tggactttcc
     2641 atcgccagta gattcgcgcg tatcacgtcg cgtatatggg tgaagtcccg ggtctgtgtt
     2701 ccgtctccgg taatggtcaa cggcactcct ttcgcacgaa gctgaaggag tttcgggatc
     2761 gcaagcgcat aggcaccctc gggatccatg ccggaaccgt acacattgaa ataccggagc
     2821 gagaccgtct cgagaccgta gagctccgag aacatgcggg catagcactc cccgatgtat
     2881 ttgtggagcc cgtagggatt caccgggagc gccggcatat cttctgaaag cggcagcgtc
     2941 gcctgttcac cgtacgccga gcttgaggct gcgtagatta cccggctgac acccgcctcc
     3001 tttgccgcaa gcagcaccga gagggtcccg ttcacattca cgtcgtgcgc ctcttgcggg
     3061 tgatcaatcg aaaacgggac gcgcgggagc gctgcctcat ggaatacggc agccgcgccc
     3121 tcgaagatct ccttgatccc tgaggtattg cgcacgtcca aaacgtgaag ggttgccccc
     3181 tcgggcaccc ggtcgcgctt tccgtcagaa aggtcatcga ccacgtgagt ctcatagccg
     3241 acgtcgatca gcgcccgcgc gatatgcgcg ccgatgaatc ccgccccacc ggtaacgacc
     3301 gccttcttca taccttatgg cggcgcgcct cctccggcac gtacggttcg taccggccgt
     3361 tcgtgtagaa gaaggcgtca tatccttcat gctggtaggt atacccgagc tgccgcgtca
     3421 ccttcccgcc atccacgacg atcgggtagg agtatccctt ccagctgccc cgcgttgtcg
     3481 ggactttgcc ccgcgacagg tgccagaaga cgaagaacgc gatccggacc atccagggga
     3541 atacggggag cgcccgcttc cccaccgcgc gcgccatatc ctcccctcgc acaattggtc
     3601 cgggcggaca aatattgaag acctcgtacg cacctgcgga gtccccgaac gcgagccgct
     3661 cgacgattcc cactacatcg tcctcatgga tgaactgccg cgcccatttc ggggtgacgg
     3721 gcacgaatgc aacgagtgcc ttgataaccg catagatgcg agcaccgcga agctgccccg
     3781 agagcgcggc ctggagccca aaacgcatgc gcatgaagcg gccgcggggg cccgtgatcg
     3841 ccgccggacg tacgatcgcg acctccgtct tcttatcgct cgcctcgtac cgctcgcgca
     3901 gatgcacctc ggcgatccgc ttctcctctg catataaata gtccgtcttc cggaacggat
     3961 cctcctcggt gaagcggtac tcgatggtgt tgtccgggaa ggagccatag gaggccacgg
     4021 tcgagaagtg aacgagacgc ttcacgaaga cgttctcgaa ggcaaagtcg aacaccgcgt
     4081 ccgagccgtc gatgttcgag cgccaggtcg cctcggtatt cccatacacc gcccgaatat
     4141 cccaggccgt atggatcacc agatcaggcc ggtatgccgc tacctgcccc tgccagctcc
     4201 cgtcttcggt gctcgcctgg atgtatacga gcttcggctc gtccttgatg aggtccggca
     4261 cgggctcgcg atcgagcccg atcacgcgct cgacatcgtc gcggcgcgca aattcctgaa
     4321 cgagcatagt accgacatag ccggccgcgc ccgtgatgag gagagtgtgc ttcatagaaa
     4381 gaggttacca gttgctcagg cggaagaaca tgcgcttgaa ggtcttgagt gcgatcacga
     4441 tatcaagcgc gagcgaccga tgcttgatgt agtaaaagtc gtaggcgagc cgcatcttcg
     4501 tctcctcgat cgactgcgga ctaatgtgcc cttgctcgta ctgctggttg atctgcgccc
     4561 atccggtgat tcccggggtc acgatatagc gcatgttgta gtacgggagc gcctcggcca
     4621 ggcggtctcc gagcgcgcgg atatcgtttc tcggaccgat gagcgaaagc tcaccggtga
     4681 gtacgttcac gaactgcggg aactcgtcga gcgaggtcat acgcaggaaa tccccgaccc
     4741 gcgtcacgcg gttctcccct tcccccggcc acatgccagt gtccgtgaag cgcatgctcc
     4801 ggaacttata ggtgcggatg ctcgaaccgc gtatcccgat gcgatcctgg acggtgaaga
     4861 gcggtccgcc gccttccagc aagttcgcgg cccatacaac aggggccgcg accattgtca
     4921 cgaggcccat gagcacaccc ccgacaagat cgatcacccg cttcacgaag gcatggaaca
     4981 caggcgtggt cgtcgttacg ttctcgagga accagccctg ggacaggagc gagagcggga
     5041 tgcgatcgaa cacctcctcg tagaccgcat caaagtcggc atagacgcag ccgtgctcga
     5101 agcagggcag catatagaga tcggggagca tggcctgcac ctcagcgcag tccgtatccg
     5161 cgacgatgag atcgatgctc ttatccccga gctcccgccg gagtccctct ctgccgagcg
     5221 cgtggagcgc agccggcgta tagaccttcc ggaactcaag gtggtagcgg cgattgccgt
     5281 tcacctcgcg cacgagttca tcggtctcct ctccttcagc aatcagtact gcataatcgc
     5341 gcacgcgggg cgcagagagc cgcggataaa gcgcgagtcg ccacgcgaag atgagggcga
     5401 gcgacacgag gaggtagatg acgaggttcg tcttcggcgc gatgcccacc cctggcacga
     5461 ggaagaagaa cagtgccgcc atgacgatat tgatcacctg ggtccggagg atggcgttcg
     5521 gcaaggagct cttgaagagg atgaaccgct tgctgtagag cccggacata tagaagacga
     5581 ggacccagag ggcgaagagg agcgaaaacg gtcccatatg gtcccggaac actgcctcgg
     5641 tcggaagcgt cccgtaccgc aggaggagcg tcagccagag cgcggccacg aaggtcgcga
     5701 ggtccccgat aaagagaagc gccgaggcgc gtatgccacc gtgggtcatg tatcgacgtt
     5761 atactataaa acgtccacaa tcacatggaa gttgccccga aacgcatact ctacgtcatc
     5821 acgcttggct ggtggggcgg cgcgcaacga tacgttttcg atctcgcgac cgcagcaaaa
     5881 agcgcgggcc atgacgtgtt cgtgctcacc ggcgagggcg agctccgcac gcgtcttgag
     5941 gaagtcgggg ttacggcctt ttccgcgact cacttccagc gcgggcttgg gctgcgcgcc
     6001 gagctcggcg cgatcacgtc gctcgggcac gccattagag cggtgcgtcc ggacatcgtg
     6061 caccttaata gctctaaggc aggattcctc ggcgccatcg tcgcccgctt ctgcggggtt
     6121 aagcgtatca tcttcaccgc gcacgggtgg gcattcagcg aagtgcgccc ggactggcag
     6181 cgcgcgatct tctggttcgc gcactacctc accctcctgc tcgtcgatcg cgtcatctgc
     6241 gtctcgcagg cggtccgtcg cggtacgcgc ttcatgcccc tcgttcaggg caagctcacg
     6301 gttatctata acggggtccc ggacatcgcg ttcttgaccc gtaaggacgc gcgcgagcag
     6361 ctcgcccccg atcttcccta tccattctgg atcggaacga tcgcggagct gcacccaaat
     6421 aaacgagtgg atgtgctgat acgggccttc gccgcgatca tggacgagtg tcctgacgca
     6481 gccgtggtga tcatgggtga aggcaccgaa cgcgagcacc tggaggcgct ggtcgctcga
     6541 ctcggcctcg aggaccgaat acggctctgc gggcacacgc cgaaggcggc aacctatctc
     6601 acggccctcg atctttttat tcttccttcc gataccgagg cgctcggcta cgtgctcatc
     6661 gaagcaggct tcgctgggct tccggtggtc gcaagcaaag tcggtgggac gccggagatc
     6721 atcgagagcg ggattgatgg cttcctcgtg cagcgccgta aggttcgagc attcgctgag
     6781 gcgatccggc agctccacga tgatccagca cttcgcgcgc gtttcggaag agcgctcaag
     6841 gagaaggtag cggcgagatt ctcccagcag cagatggtcg acaaaacctt gatcctctac
     6901 cgttagccct cgtactcctg catcgccgcg tcccgcgggg ccgcgcgtcc gtgccgggcc
     6961 aaggacgcca cgatccccaa gcccgcaaac tccatgagga ggtgcgagcc gccatagctc
     7021 atgaacggga tggtggtgcc ggtgaccggc aggagcccca ggttgatgcc cacgtgaatg
     7081 aaaatatgcg cgaacaggac cagcgtcacc ccaatcgtga agaaggcgtc gaagttcgtc
     7141 gccgcgcgct gcgcgatcgc gacgagccgg agcaggagga gcgcatacag aatgagcacc
     7201 agcgtgatcc cgatgaatcc ccactcctcg gcgaacgccg cgaagatgaa atccgtctcg
     7261 tactctggca ggaaccggag cttggattgg gtgccgtacc cgataccctt ccccatgagt
     7321 tcccccgacc cgaccgcaat cgtcgcctgg taggcgttgt acccggaacc ccggatatcg
     7381 gccgcgggat tcaggaagga tacgatgcgc tcgcgctggt actcctcgag cgcaaacgcc
     7441 cacagcatgc ccgcgccgat caccccggcc gcaaggatta gggcgagatg cttcttcgag
     7501 atgcccgaga cgaggatcat cccgaaccac acgacaccga ggatggccgc ggtgccgagg
     7561 tccggctgca caagaataag caggatgggg atgccggcgt agagggcgga gacgagcacg
     7621 tgccggaagt tcccgatctc catgtgccgc cgggagaagt acttcgcaag cagcgccacg
     7681 aacacgatct tcgccggatc cgacggctgg aaggagaagg ccccgagatc gaaccaggac
     7741 ttggcgccct ggaccgcgtg ggtcgcgacc aagaggagcg caagcagccc caataccacg
     7801 acgtaggccg agacgaggac accggtccgc ctgatgaacc gcatatcgag cgcggagcat
     7861 acgaggaaca ccagcgtcgc gcccgcaatc cagatgagct ggcggggcgc caaggacgcc
     7921 tcctcaccga acggatgcat ggtcaggacg ccaaggaggc agagcgcaag cgggataagg
     7981 aggagcggcc actcagacac gagcgcggca agcggattcc ggcgcacctg ctcgctcata
     8041 acggcacgat gggcacgacg tcgatgcgcg ccggcgccgc gctaaacggc gcattccaga
     8101 cgaacaccac ctgcgcgctc ccctggggcg gaagctccgc aagcagggtc tgcgtcgccg
     8161 cgatcgcgtt cccgtcagca tcgaacacgg tcgcgatgac ggggatgttc cggagcgccg
     8221 tcgccgacgg gttcgagaag ctcgccatga tgcgcgggcc atcggtcccc atgacctgcg
     8281 catcgttgtt gtagcgcggc acgatgcgcc gatcctggta cgtcacgaag acgagcgagg
     8341 cgggatcaaa gacgaagaag gcgcgcgtgc cggcctcatc cgcaggaagg cccggcacga
     8401 agacagggac agtcgctccc ggcggcaggt ccatcgtccc ctgccgcgtc gcgacgatgc
     8461 tcccggcgct cccgtagagt tcgacggtgt agcgtgcgct gcgcaccgcc gcctctttgt
     8521 tcgggttcgc cacgtacgcg atgacatcgg tgcggcccgc ccgcggcgat acgatccgcg
     8581 cgaactccac cttcggcgag agcgcttccg cctcgcacac ccgcgcgcag gagccgccgc
     8641 aatccacgcc ggtctcgtcc tggttcctct tcttgtccga gcaggtaggg gtctcgtaga
     8701 cgatggggat gacgatcgcc gcgagtaagg cgatcgcgat cgctgcgagt atggaaatga
     8761 cgatgaagcg gcggcgggag gcccaggaca tgaggatagt atataggaaa aaggccgccg
     8821 tccctcccag ggaagcggcg gcagttgagg cttggccggt gtgtgcacga gccgcgcctc
     8881 cgttcatgaa agcatgtccg ggacaaatgc aaaatgccgc ccctgcgggc ggctcgtgcg
     8941 ttgtaaaaca tgaacggaac acgcccatga agctggcgac aacctactct cccctttacg
     9001 agtaccatcg gctcaagagg gcttaactgc cgtgttcgga atgagaacgg gtgtggcccc
     9061 tccgataaat caccagcttc atgggcgcgt tcgcgaccac tatgcaaaat aggcgcacaa
     9121 ttcaccaaga gcatcgcatt gaagtgatac tcacggttcc gcacaagaga ctgggcatat
     9181 tagtactcct cgactgaaca cattgctgtg cgtacatcta gagcctatca acctagtaat
     9241 cttctaggag cctcataacg attccttatc tttgagcgcg cttcccgctt atatgctttc
     9301 agcagttatc gcttcccgac atagctaccc agcggtgccc ttggcaggac agctggtaga
     9361 ccagaggtca gttcaccccg gtcctctcgt actaggggca agcctcatca agaatcaacg
     9421 cctgtagtag ataggagacc aacctgtctc acgcatttcg ttcgccgaga gagtgattag
     9481 ttcttctcgg gcttgctccg attactcgga ggatcggact gtaacttcat cgggtctcga
     9541 tgatcaacat tcagtctcta cgggcggcgg ttgccttccc tcggtattgc ccttcccgtg
     9601 cgggagtcgg gttctaccga tatcagttga tggtacgtac ggcattcctg ccgcacgtcg
     9661 ccgtgtcatg gttggttcct atgctttctc aaagacgaaa agacactatt ggttttcgat
     9721 aacatctcta tggggttcaa acgggtcgag catccgatac acatcaggtg ctcgacggtt
     9781 tgaacccagc tcgcgtacct ttttaattgg cgaacagcca aacgcttggg accttctcca
     9841 gccccgcgct aaggtgagcc gacatcgagg tgccgaactc cgccgtcgct atggacgctt
     9901 gggcggaact agcctgttat ccccagggta acttttatcc gttaatctct ggccgcatct
     9961 aaggcggacc atcggttcac ta
//
LOCUS       DASPKG010000048        11524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_623119, whole genome shotgun sequence.
ACCESSION   DASPKG010000048 DASPKG010000000
VERSION     DASPKG010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11524)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11524
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623119"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2009
                     /locus_tag="VEA36_01815"
     CDS             <1..2009
                     /locus_tag="VEA36_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HYF29084.1"
                     /translation="SVATEIPLQATIRRLSRLIIIVVAVMSALLFALGLALGQSATTM
                     FSTVVSLAVSVIPEGLPIVMTLVLATGVWRMGKRNALVKNLQAVEALGQASVIAVDKT
                     GTITKNEMIVEKVYAEGTLFTVSGEGYEPKGEVAKDGSIIVPANHPELLAAGKIAAYI
                     AGARVVYSEEKKRWMVAGDPTEAALIVLARKLGFEKEDLERESLPIAELPFTSALRFH
                     AVVHAGMREGALSVIGAPEHILARAVRVRENGKTYELTGTRRDELDALMRSLSVEGLR
                     VLAYAERASVGANLSPDAVADLTFLGFIGMKDGLKAEVPAALAKARAAGIRVVMITGD
                     HVVTAEAVAKEAGIFKEGNRVVSGTELDSLSDAALADILLTVAVFARVTPEHKLRIIQ
                     AYRAKGLTIAMTGDGINDAPSLVAADLGVSMGKIGTEVAKEAADIVLLDDDFGSIVSA
                     VEEGRNIYQNIRRVALYLFSTSAGETLTIIGALFARLPVPVLPAQIIWLNFVTDGFLD
                     VALAMEPKEKNLLTRPFRKPGKYLLDSSMTLRMALMAATMAAVTLPLFALFLSTEGIA
                     KALTISLTCLAAMQWYNAWNCRSETRSLFAMNPFGNLYLIGATVVVVLLQLLAVYHPF
                     FQGVLHTVPLSLAEWQLVLVAGLPIILVEEARKLVARLTLRLSH"
     gene            2046..2924
                     /locus_tag="VEA36_01820"
     CDS             2046..2924
                     /locus_tag="VEA36_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYF29085.1"
                     /translation="MRNRKIEQKKKIPPQVQGVQPGRTAPMQPAPEDKMECYEGSGKL
                     SGKIALITGGDSGIGRATAIAFAKEGASVAIIYCNEDDDAKETAHHIEDAAGEGQVTL
                     LRADIGKPAVARRVVEQIVKRLGGLDILVNNAAEQHSQPSLVDITPDQLERTFRTNVF
                     GMFYMTQAALQYLKPGSVIINTTSVTAYKGSEDLIDYSATKGAIVAFTRSLALSLAEK
                     KIRVNAVAPGPIWTPLIPASFPKERVGKFGQNTTLGRPGHPDEIAPSFVFLASDDSSY
                     MTGQVLHPNGGTIVNG"
     gene            2983..3522
                     /locus_tag="VEA36_01825"
     CDS             2983..3522
                     /locus_tag="VEA36_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29086.1"
                     /translation="MKKFTIGMFGAREDAEKAINRLHNELKVPTDDISYIYRNSEGEV
                     HEVEASNIATSTPVEGAGKGAVVGGALGAIAGLATVAGVIPVIGPLFAAGPLAAALGL
                     TGAIGTTAAGAATGAAAGGLIGALSNMGIGQEHAQRYADRVNAGDILLATYADENVDA
                     EALLEDSGAIDVETYRLAV"
     gene            3588..4202
                     /locus_tag="VEA36_01830"
     CDS             3588..4202
                     /locus_tag="VEA36_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29087.1"
                     /translation="MSVVLRNTIVDWDKVRGPIFMCLGPGPGAQDWELDCYRLLEAHY
                     GNDFLLHITRKYADDHPLMAYRTPTVETIDGRSLEQETDAKRYYIAQAGWSNRLPRGC
                     IICWLPLEDPKHPCLEEEGPYAHEARDLIGEIRGRMIELSKERRGHVRFVIGADENFP
                     GLASIRRNFERAERIPRITIYSTLEKTVEAAVAMTTPIRVAASA"
     gene            4288..5943
                     /locus_tag="VEA36_01835"
     CDS             4288..5943
                     /locus_tag="VEA36_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYF29088.1"
                     /translation="MTQKEALSILKTGANVFLTGEPGSGKTHTINEYVKWLRSHAIEP
                     AITASTGIAATHLHGVTIHSWSGIGIAEQPLAQLIEQITQKEHVVKRIQKTPVLIIDE
                     VSMLSGDVLTMVDAVVRAVRQREEPFGGMQVVVVGDFFQLPPIGRPGRPASFAFESPA
                     WAALKPIVCYLSEQHRQEDTEFLSLLGAIRASEWDHTHVSVILSRETDAEGLDADTPR
                     LYTHNADVDRENEAKLGALPGNGKRYAMTGQGAPVLIESLKRGCLSPETLVLKEGAVV
                     MATKNNPITGYANGTIGVVTGFERGTGYPTIETKDGRELTIAPVEWAVEEGGKARAKI
                     TQVPLRLAWAITVHKSQGMSMDAAAMDLSRSFEYGQGYVALSRVRRLSGLSLLGWHEN
                     ALAVHPGVASRDRQFRALSEQAAVAFGMLAETGELDTMRNNFIKASGGSLEASALPKA
                     KQTTYDATYDLIKEGKSVAEIKAARGLTLGTIADHMEKLKAGGRITLEEIRSILPKKI
                     IDAMPAISEVFATVGTEKLAPAHSRLKGKYSYDDLKLARLLHV"
     gene            complement(5986..7398)
                     /gene="purB"
                     /locus_tag="VEA36_01840"
     CDS             complement(5986..7398)
                     /gene="purB"
                     /locus_tag="VEA36_01840"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HYF29089.1"
                     /translation="MTERIGNVLAERYATVEMQTIWSDRAKIIAERRLWIAVMKAQRD
                     LGLAQITAAAIADYERVIDEVDFDSIDRREKKAKHDVKARVEEFNALAGHQLAHLGMT
                     SRDVTDNVEQMQVRDSLRLVSDRTVALLRRLGARILEHQVLTACGRTHNIPAQSTTLG
                     RRFGMWAEEHITHFELLDKFRGGYLLRGIKGPVGTQQDMIALIGTNEAARLEDIVRKH
                     LGFGGTLTAVGQVYPRSFDTATVAHLVTLASAPANFAKTMRNMAGLAQATEGFKEGQT
                     GSSAMPHKMNMRTSERISGLNVVLHGFATMANALAGDQWYEGDVSCSVPRRVFLPSSF
                     FAIDGLYEAAMTVLDEMAVFPAMIERELMAELPFLSTTGILMEAVKRSGGREDMHEIV
                     KKHATASVTAVRSGGSSDAFVVSLGDDPAFPLSTEEIRALLKPEVGLAIEQADHVYQR
                     IRRIVEHRPDAARYNPAPIR"
     gene            7504..8502
                     /gene="mltG"
                     /locus_tag="VEA36_01845"
     CDS             7504..8502
                     /gene="mltG"
                     /locus_tag="VEA36_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HYF29090.1"
                     /translation="MRFPFSLSERFDRRAVALTAIGGILVIVLLLLAYSAMFGPVAKY
                     DEPEEFIVNPDTPIEEVAAGLKELNFIKSELAFRLAYAATGHDDILEGGYRISRSQDA
                     WTIAEAFARPPYLSWVIVPPGMRKEQIGELLQRKLGWSEEQLAEWGRQTAVYETLREG
                     VYYGDTYLIASDQPIGQVANRLRGRFEEVFAPYADEAAKKGLSWNEVITMASLVERES
                     AKNDKALVAGILWNRIDIGMALQVDATLQYIRGRSGLWWPTPMSDDKFIDSPFNTYKY
                     PGLPPHAIANPSLDSIKAVLDPEETNCIYYLHDNDGQIHCSASYAGQLRNVDRYLR"
     gene            complement(8550..9218)
                     /locus_tag="VEA36_01850"
     CDS             complement(8550..9218)
                     /locus_tag="VEA36_01850"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYF29091.1"
                     /translation="MPRIIKAILCDVDGTIIDSFSRGFYKLRLAAERFGYDYTEETER
                     IAIEHWGAPLDKLLLICFPDVTSETARKMRRLFVDLDTTEPPGAIAGALAVLELLAKE
                     GIVFTVLTSRDSVTCGHLLRNAAMDHYFIHVAAQDTTEHHKPDPRVFACTMEKLKEHG
                     IEPDECVLIGDTYDDWKAGQAFGMRTVIVKTGPLMHPTADIPEEDHIQSFAELPDWLS
                     RNNF"
     gene            9328..11112
                     /gene="typA"
                     /locus_tag="VEA36_01855"
     CDS             9328..11112
                     /gene="typA"
                     /locus_tag="VEA36_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HYF29092.1"
                     /translation="MDIRNIAIIAHVDHGKTTLTDAILKQTGAAAEGVSMDSNTLEQE
                     RGITIYSKNAAVEYKGTKINIVDTPGHSDFGSEVERVLRSIDSVLLIVDAQEGPMPQT
                     RFVLKKSLELGLKPIVVLNKIDKPAANPALAEEQVLELFLELGASDEQSDFTTVYAIG
                     REGVAMKKLDDERKDLTPLLDVILEQVPVASSDEKASKPLRLQPFNLAYDNFLGRLAI
                     GRVYEGVVKAGETVTVKKPDGTARTGKITKIFTFKGLERIEAESASAGDIVIAAGLPD
                     IYIGETITTDPSAEPLPAIAIDEPTITLNFLVNNSPFAGREGKYVTSRQLREYLEREL
                     EVNVGLKVDFVSPDAFKVSGRGELHIAILLENMRRAGYEMQVFQPQVIIREEEGVKVE
                     PFEEVIIDTPSDYQGAIIERLGQRAFILQNLVQHDNSVRLTLEGPTRGLLGYRNQFVI
                     DTKGEGILSSRVIGFKPFAGEIKKRQVGSMVSMATGKALGYALWGLQERGMLYIGPAT
                     DVYEGMVLGNTSKGEEMIVNPTKGKNLTNVRSSGNDEAINLVPAYTLTIERGLEVMAE
                     DEYLEITPESVRLRKQQLTESERAKARR"
     gene            11200..11352
                     /locus_tag="VEA36_01860"
     CDS             11200..11352
                     /locus_tag="VEA36_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HYF29093.1"
                     /translation="MMYTIAVILLVLWLLGLVTSYTLGGFIHLLLVVAVIMVLVRLIQ
                     GRNPIA"
     gene            11364..>11524
                     /locus_tag="VEA36_01865"
     CDS             11364..>11524
                     /locus_tag="VEA36_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HYF29094.1"
                     /translation="MPRGSKAKYTDKQKRQARHIEEGYEKRGVSEDEAEARAWATVNK
                     MTGGGKKSGS"
BASE COUNT         2160 a         3672 c         3680 g         2012 t
ORIGIN      
        1 agtcggtcgc gaccgagatc ccgctccagg cgacgatacg ccgcctctcg cggctcatca
       61 tcatcgtggt cgcggtgatg agcgccctcc tgttcgcgct cgggctcgcg ctcgggcaat
      121 cggccacgac catgttctcg acggtggtct cgcttgcggt ctcggtcatc ccggaaggcc
      181 tcccgatcgt catgaccctc gtgctcgcga ccggcgtctg gcggatgggc aagcgcaacg
      241 cgctcgtgaa gaacctgcag gcggtcgagg cactcggcca ggcgagcgtg atcgcggtcg
      301 acaagaccgg cacgatcacg aagaacgaga tgatcgtcga gaaggtgtac gccgagggca
      361 ccctcttcac ggtctcgggc gagggctacg aacccaaggg cgaggtagcc aaggacggaa
      421 gcatcattgt ccccgcgaac catccggaac tgcttgcggc cgggaagatc gcggcataca
      481 tcgcaggcgc ccgcgtcgtc tacagcgaag agaagaagcg ctggatggtt gcgggcgacc
      541 cgaccgaggc cgcgctcatc gtgctcgccc gcaagctcgg gttcgagaag gaagacctcg
      601 agcgcgaatc gctcccgatc gccgagctcc cgttcacctc cgcgctgcgc ttccatgcgg
      661 tggtgcatgc ggggatgcgc gaaggagcgc tctccgtgat cggcgcgccc gagcacatcc
      721 ttgcgcgtgc cgtccgggtc cgggagaacg ggaagacgta cgaactgacg ggcacgcgcc
      781 gcgacgagct cgatgccttg atgcgctcgc tctccgtcga ggggctgcgc gtcctcgcgt
      841 acgcggagcg cgccagcgtc ggggcgaacc tctctccgga tgcggtcgcg gacctcacct
      901 tcctcggctt catcggcatg aaggacgggc tgaaggccga ggtcccggcc gcgctcgcca
      961 aggcgcgcgc cgccggcatc cgcgtcgtga tgatcacggg cgatcacgtc gtgaccgccg
     1021 aagccgtcgc gaaggaggcc ggcatcttca aagaagggaa tcgggtcgtt tcgggcaccg
     1081 agctcgattc gctctcggac gcggcgctcg ccgatatcct gcttaccgtc gcggtcttcg
     1141 cccgcgtgac gcccgagcat aagctgcgca tcatccaggc gtaccgcgcg aagggcctca
     1201 ccatcgcgat gacgggcgac ggcatcaacg atgcgccgtc cttggtcgcg gcggacctcg
     1261 gcgtctccat ggggaagatc gggacggagg tcgcgaaaga ggccgcggac atcgtgctcc
     1321 tcgatgatga tttcggctcc atcgtctcgg cggtcgagga gggcaggaac atctaccaga
     1381 acatccgccg cgtcgcgctc tacctcttct cgacgtccgc cggcgagacg ctcaccatca
     1441 tcggggccct gttcgcgcgc ctgccggtcc ccgtgctgcc cgcgcagatc atctggctca
     1501 acttcgtgac cgacggcttc ctcgacgtcg cgctcgccat ggagcccaag gaaaagaacc
     1561 ttctcacgcg tccgttcagg aagcccggga aatacctcct cgactcctcg atgacgctcc
     1621 ggatggcgct gatggccgcg acgatggcgg cggtgacgct gcccctgttc gcgctcttcc
     1681 tctcgaccga gggcatcgcc aaggcgctca ccatatccct gacctgcctc gcagcgatgc
     1741 agtggtacaa cgcctggaac tgccgctcgg agacccgctc gctatttgcc atgaatccct
     1801 tcgggaacct ctacctcatc ggcgcgacgg tcgtggtcgt gcttctccag ctcctcgccg
     1861 tgtaccaccc cttcttccag ggcgtgctgc acaccgtccc gctctcgctt gcggaatggc
     1921 agctcgtcct tgtcgccggg ctcccgatca tcctcgtcga ggaggcacgc aagctcgtcg
     1981 cgcgcctgac gctgcgcctt tcccattaat taccgcttca cggttcattc cgaactatag
     2041 acggaatgcg caatcggaag atcgagcaaa agaagaagat cccgccgcag gtccagggcg
     2101 tgcagccggg gcgtaccgcg cccatgcagc ctgcgcccga ggacaagatg gagtgctacg
     2161 aagggagcgg gaagctctcc gggaagatcg cgctcatcac gggcggggac agcggcatcg
     2221 ggcgggccac cgcgatcgca ttcgcgaaag aaggagcttc cgtcgccatc atctattgca
     2281 atgaggacga tgatgcgaag gagactgcgc accatataga agatgcggca ggcgaagggc
     2341 aggtgaccct gcttcgcgcg gatatcggga aacccgcagt cgcccgaaga gtcgtcgagc
     2401 agatcgtgaa gcggctcggc gggctcgaca tcctcgtgaa caatgcggcc gagcagcatt
     2461 cgcagccgtc cctggtcgat atcactcccg accagctcga gcgcaccttc cggacgaacg
     2521 tcttcggcat gttctatatg acccaggccg ccctgcagta cctgaagccc ggcagcgtga
     2581 tcatcaatac gacctcggta acggcatata aagggagcga ggacctcatc gactattccg
     2641 cgaccaaggg cgcgatcgtg gcgttcacgc gctcgctcgc gctgtcgctc gcagagaaga
     2701 agatccgggt gaatgcggtc gcgcccggtc ccatctggac cccgctcatc cccgcgtcct
     2761 tcccgaagga gcgggtgggg aagttcggcc agaacacgac cttgggtcgc ccggggcatc
     2821 cggatgagat cgcgccctcg ttcgtcttcc tcgcctccga cgactcctcg tacatgacgg
     2881 ggcaggtcct gcatccgaat ggcgggacga tcgtaaatgg ttaatcggcc gttcacgccc
     2941 cctctgctat cactcaagca ttaccacttt cacatcgtat ccatgaagaa gttcaccatc
     3001 ggcatgttcg gtgcgcgcga ggacgcggag aaggccatta atcggttgca taacgagctc
     3061 aaggtcccga ctgatgatat ctcgtacatc taccgcaata gcgaagggga ggtgcacgag
     3121 gtcgaggcaa gcaacatcgc gacctcgacc ccggtcgaag gcgccggcaa gggtgcggtc
     3181 gtcggtggcg cgcttggcgc gatcgcgggc ctcgctaccg tcgcgggcgt gatcccggtc
     3241 atcgggccct tgttcgcggc cggcccgctt gcggcggcgc ttggcctcac gggcgcgatc
     3301 ggcacgaccg cggcaggggc agcgaccggc gccgctgcag gcggactcat cggcgcgctc
     3361 tccaatatgg gtatcgggca agagcatgcg cagcggtatg ccgaccgcgt gaacgcgggc
     3421 gatatcctgc ttgcgaccta cgcggacgag aacgtggacg ccgaggcgct ccttgaagac
     3481 tcgggcgcaa tcgacgtcga gacgtaccgg cttgcggtct aggctatcct ttggcgaaac
     3541 cctgggggag gagtatcgta attacagaag caacagggga ggcggccatg agcgtcgtcc
     3601 tgcgtaacac catcgttgat tgggacaaag tgcgggggcc gatattcatg tgcctcggtc
     3661 ccggtccggg tgcgcaggac tgggagctcg actgctaccg tctgctcgaa gcgcactacg
     3721 gcaacgactt cctcctgcac atcacgcgca agtatgcaga cgatcatccg ctcatggcgt
     3781 accggacgcc gacagtggag accatcgacg gacggtcgct cgagcaggaa acggatgcca
     3841 agcggtacta catcgcgcag gctggctggt caaaccgcct tcctaggggc tgcatcatct
     3901 gctggttgcc gctcgaggat ccgaagcatc cgtgccttga ggaagaaggg ccctacgccc
     3961 atgaggcccg cgatctgatc ggcgagatcc ggggtcgcat gatcgagctc tccaaggaac
     4021 ggcgcgggca cgtgcggttc gtcatcgggg ccgacgagaa cttcccgggg ctcgcctcca
     4081 tacggaggaa cttcgaacgc gccgagcgga ttccacgcat taccatctac tccacgctgg
     4141 agaagacggt cgaagcagcg gtcgccatga ccacgccgat acgtgtcgcc gcatcagctt
     4201 aggaaagggg agcgccgccc gcatgtcggg cggcaacctt tatcttttct ccagatgcat
     4261 ttgatccgct tctggtagta tcgggggatg acgcagaagg aagccctctc catcctcaag
     4321 accggcgcga acgtgttcct caccggggag ccaggcagcg gcaagacgca taccattaat
     4381 gagtacgtga agtggctgcg ctcgcacgcc atcgagcccg cgatcaccgc ctcgacgggc
     4441 atcgcggcca cccacctcca cggcgtcacc atccactcct ggagcgggat cggcatcgcc
     4501 gagcagccgc tcgcccagct catcgagcag atcacccaga aggagcatgt cgtgaagcgc
     4561 atccagaaga ccccggtcct catcatcgat gaggtatcca tgctctcggg cgacgtcctc
     4621 accatggtgg acgcggtcgt gcgcgcggtg cggcagcggg aggagccctt cggcggcatg
     4681 caggtcgtcg tcgtcggcga cttcttccag ctcccgccca tcgggaggcc cggccgcccg
     4741 gcatccttcg ccttcgaatc tcccgcatgg gcggccctga agcccatcgt ctgctatctc
     4801 tccgaacagc accggcagga ggatacggag ttcctttcgc tccttggcgc catccgggcg
     4861 agcgaatggg accatacgca tgtgtcggtc atcctctcgc gtgagacgga tgccgaaggg
     4921 ctcgatgcgg ataccccgcg cctctatacc cataacgcgg acgtggaccg tgagaacgag
     4981 gcgaagctcg gggcgctccc cggcaacgga aagcgctacg ccatgaccgg acagggcgcg
     5041 cccgtcctca tcgagagcct gaagcgcggc tgcctctcgc ccgagacgct tgtcctcaaa
     5101 gagggtgcgg tcgtcatggc gacgaagaac aatcccatca ccggatacgc caacggcacc
     5161 atcggcgtgg tgacgggctt cgagcgcggc acggggtatc ccaccatcga gacgaaggac
     5221 gggcgcgagc tcaccatcgc gccggtcgag tgggcggtcg aagaaggggg gaaggcgcgc
     5281 gcgaagatca cgcaggtacc gctccggctc gcctgggcca tcaccgtgca taagagccag
     5341 ggcatgagca tggacgcggc ggccatggac ctgtcacgct ccttcgagta cgggcaggga
     5401 tacgttgcct tgtcgcgggt gcgcaggctc tcgggcctct cgctgcttgg ctggcatgag
     5461 aacgcgcttg ccgtgcatcc cggggtcgcg tcgagggacc gccagtttcg cgcgctttca
     5521 gagcaggcag cggtcgcgtt cgggatgctc gccgagacgg gcgagctcga tacgatgcgg
     5581 aataatttca tcaaggcctc cggcggctcg ctcgaggcgt ctgcgctccc gaaggcgaag
     5641 cagacgacct acgacgcgac ctacgacctc atcaaggagg ggaagagcgt tgcggagatc
     5701 aaggcggcgc gcgggcttac gctcggcacc atcgcggatc atatggagaa gctgaaggca
     5761 gggggacgca tcacgctcga ggagatacgc agcatcctcc cgaagaagat catcgatgcg
     5821 atgcctgcga tcagcgaggt attcgctacg gtcgggacgg agaagctcgc gccggcgcac
     5881 tcaagactga aaggcaaata ttcctacgat gatttgaagc tcgcccggct gctccatgta
     5941 taaaagggtc cgccaggacc acaggggccc tggcggtacg cgaacttagc ggatcggcgc
     6001 ggggttgtac cgtgccgcat ccggccgatg ctcgacgatg cggcggatcc gctgatagac
     6061 gtggtccgcc tgctcgatcg caaggccgac ctccggcttc aggagcgccc ggatctcctc
     6121 ggtgctgagc gggaacgcgg ggtcgtcccc gaggctcacg acgaacgcat cggacgaccc
     6181 gccggagcgg accgcggtga cagacgctgt cgcgtgcttc ttgacgatct catgcatgtc
     6241 ctcgcgcccg ccgctccgtt tgaccgcctc catgaggatg ccggtcgtcg agaggaaggg
     6301 gagctcggcc atgagctcgc gctcgatcat cgccgggaag accgccatct cgtcgaggac
     6361 ggtcatggcg gcctcgtaga gcccgtcaat agcgaagaac gaactgggca ggaacacccg
     6421 gcgcggcacc gagcagctca cgtctccctc gtaccattgg tcccccgcaa gggcgttcgc
     6481 catggtcgcg aagccgtgca ggacgacgtt gaggccgctg atgcgctccg aggtccgcat
     6541 gttcatctta tgcggcatcg cggaggagcc ggtctggccc tccttgaacc cctcggtcgc
     6601 ctgggcgagc cccgccatgt tccgcatcgt cttcgcgaag ttcgcgggcg ccgaggcgag
     6661 cgtgaccaga tgcgccacgg tcgcggtgtc gaacgaacgc ggatagacct ggccgacggc
     6721 ggtgagcgtg ccgccgaagc cgaggtgctt gcggacgatg tcctcgagcc gcgccgcttc
     6781 gttggtgccg atgagcgcga tcatgtcctg ctgcgtcccc accgggcctt tgatgccccg
     6841 gaggagatag ccgccgcgga atttgtcgag gagctcgaaa tgcgtgatgt gttcctctgc
     6901 ccacattccg aaccgcctcc cgagcgtcgt ggactgcgcc gggatattat gcgtcctgcc
     6961 gcaggccgtc agcacctggt gttcgaggat acgtgcgccg agccgccgca ggagcgcgac
     7021 cgttcggtcg ctcaccaggc ggagcgagtc gcggacctgc atctgctcga cgttgtcggt
     7081 cacatcgcgg gacgtcatgc ctaggtgggc gagctgatgg ccggcgagcg cgttgaactc
     7141 ctcgacgcgc gccttcacgt catgcttggc cttcttctcg cggcgatcga tcgaatcgaa
     7201 atcgacctca tcgatcacgc gctcatagtc ggcgattgca gcagcggtga tctgggcgag
     7261 gccgagatcg cgctgcgctt tcattacggc gatccagagt cgccgttcgg cgatgatctt
     7321 ggcccgatcg gaccagatcg tctgcatctc gacggtcgca tagcgctcgg cgagcacgtt
     7381 accgatcctc tcggtcatgg ctgttccttc tcagtgttcg gccggccaaa tccggttccg
     7441 cagggatagt acctttttgg gtatcttctg caatctgctg agccgatgcg gtagaatgcg
     7501 ggaatgcgtt tcccgttctc cctctccgag cggttcgacc gccgtgcggt cgcgctcacc
     7561 gccatcggag ggatcctcgt catcgtgctg ctcttgctcg cgtattccgc catgttcggg
     7621 ccggtcgcta aatacgatga gcccgaggag ttcatcgtga acccggacac cccgatagaa
     7681 gaagtggcgg cgggattgaa agagctgaac ttcatcaaga gcgagctggc gttccgcctt
     7741 gcgtacgcgg cgaccggcca cgacgacatc ctcgaaggcg ggtaccgcat cagcaggagc
     7801 caggacgcgt ggaccatcgc ggaggccttc gcccgtccgc cctacctctc ctgggtcatc
     7861 gtgccgccgg ggatgcgcaa ggagcagatc ggggagctgc tgcagcggaa gctcgggtgg
     7921 tcggaggagc agctcgccga gtggggaagg cagaccgcgg tatacgagac gcttcgcgag
     7981 ggcgtctact acggcgacac ctacctcatc gcctcggacc agccgatcgg gcaggtggcc
     8041 aatcgactgc gcggccgctt cgaggaggtg ttcgcgccgt atgcggacga ggcggcgaag
     8101 aagggcctct cctggaacga ggtgatcacc atggcctcct tggtggagcg cgagagcgcg
     8161 aagaacgata aggcgctcgt cgcaggcatc ctctggaacc gcatcgatat cgggatggcg
     8221 ctccaggtgg acgcgacgct ccagtacatc cgggggaggt cggggctctg gtggccgact
     8281 cctatgtccg acgataagtt catcgattcg cccttcaata cgtataaata cccggggctc
     8341 ccgccgcatg cgatcgcgaa cccctcgctc gactccatca aggcggtgct cgatcccgaa
     8401 gagacgaact gcatttacta tctccacgac aacgatgggc agatccattg ctcagcgagc
     8461 tatgcggggc agctgcggaa tgtcgaccga tatctgcggt aataaaaagt aagagcccgt
     8521 tacgcgggct ccggcgacac attcgacgcc taaaaattgt tccgagacag ccagtccggc
     8581 agctcggcga aggactgaat atggtcctcc tccggaatgt cggctgtcgg atgcatgagc
     8641 ggtccagttt tcacgatgac cgtacgcatg ccgaatgcct gcccggcttt ccagtcgtcg
     8701 taggtgtcgc cgatgaggac gcactcatcg ggttcgatgc cgtgctcctt cagcttctcc
     8761 atggtgcagg cgaagacgcg cggatccggc ttgtggtgct cggttgtatc ctgcgcagcg
     8821 acatggatga agtaatgatc catggcggcg ttgcgcagca gatggccgca ggtcacgctg
     8881 tcgcgcgagg tgagcactgt gaatacgatg ccttccttgg cgagcaactc gagaaccgca
     8941 agcgcgccgg cgattgcgcc gggcggctcg gtcgtgtcga ggtcgacgaa gaggcgcctc
     9001 atcttgcggg ccgtctcgga cgtgacatcc gggaagcaga tcaggagaag cttatcgagc
     9061 ggcgcgcccc agtgctcgat ggcgatcctc tcggtctcct cggtgtagtc gtagccgaac
     9121 cgctcggcag ccagccgaag cttgtagaat ccacggctga acgaatcgat aatggtgcca
     9181 tccacatcgc agaggatggc cttgatgata cggggcatgt cgccaccttt ctgttgaccc
     9241 atagcggtta tagcacgcag gtcggattga cgcactctca tatccgggtt attctctccc
     9301 caacagcccg ccgggccttt tatttttatg gacatccgca acatcgcgat catcgcgcac
     9361 gtcgaccacg ggaagacgac cctgaccgat gcgatcctga agcagaccgg cgcggcggct
     9421 gagggcgtct ccatggactc caacacgctc gagcaggagc gcggcatcac catctactcg
     9481 aagaacgccg cggtcgagta caagggcacg aagatcaaca tcgtggacac gccagggcac
     9541 tccgacttcg ggtcggaggt ggagcgcgtg ctccgttcga tcgactccgt gctcctcatc
     9601 gtggacgcgc aggaaggccc catgccccag acccgcttcg tgctgaagaa atccctcgag
     9661 ctgggcctga agcccatcgt ggtcctcaat aagatcgata agccggccgc aaaccccgcg
     9721 ctcgccgagg aacaggtctt ggagctcttc ctcgagctcg gtgcctccga cgagcaatcg
     9781 gacttcacga cggtctatgc gatcgggcgc gagggcgtag cgatgaagaa gctcgacgac
     9841 gagcgtaagg acctcacgcc gctcctcgac gtcatcctcg agcaggtgcc ggtggcgtcg
     9901 tccgacgaga aggcctcgaa gccacttcgc ctgcagccct tcaaccttgc ttacgacaac
     9961 ttcctcggtc gcctcgcgat cggccgtgtg tacgagggcg tcgtgaaggc gggcgagacc
    10021 gtgacggtga agaaaccgga cggtaccgct cgtactggga agatcaccaa gatcttcacc
    10081 ttcaaggggc tcgagcgcat cgaggcagag tccgcgtccg caggcgacat cgtgatcgcc
    10141 gcagggctcc ctgacatcta tattggcgag accattacga ccgatccttc tgccgagccc
    10201 ttgcccgcga tcgcgattga cgagccaacc atcaccctca acttcctcgt gaacaactcg
    10261 ccgttcgcgg gccgcgaagg gaagtacgtg acgagccgcc agctccgcga atacctcgag
    10321 cgcgagctcg aggtgaacgt agggctcaag gtggacttcg tctcaccgga tgccttcaag
    10381 gtgtcgggcc gcggcgagct ccacatcgcg atccttctcg agaacatgcg ccgtgcgggc
    10441 tacgagatgc aggtcttcca gccgcaggtc atcatccgcg aggaggaggg cgtgaaggtg
    10501 gagcccttcg aggaggtcat catcgatacg ccctcggatt accagggcgc cattatcgag
    10561 cgactggggc agcgcgcatt tatcctccag aacctcgtcc agcacgacaa ctcggtgcgg
    10621 ctcacgctcg agggcccgac ccgcggcctc ctcgggtacc gcaaccagtt cgtcatcgat
    10681 accaagggcg aaggcatcct ctcgtcccgt gtgatcggtt tcaagccgtt cgccggcgag
    10741 atcaagaagc gccaggtcgg ctcgatggtc tccatggcca ccggcaaggc gctcggctat
    10801 gcgctctggg ggctgcagga gcgcggaatg ctctacatcg gtccggcgac cgacgtctat
    10861 gagggcatgg tgctcggaaa caccagcaag ggggaggaga tgattgtgaa cccgaccaag
    10921 ggcaagaacc tcacgaacgt gcgctcctcc ggcaacgacg aggcgatcaa cctcgtgccg
    10981 gcctacacgc tcaccatcga gcgtgggctc gaggtcatgg ccgaggacga gtatctcgag
    11041 atcacgcccg agagcgtacg gctccggaag cagcagctca cggagagcga acgagcaaag
    11101 gctcgtcggt aatatgaagg cttgcggaag caagccttca tcatttctct accttccttc
    11161 gtctaccgta cccagtgtta gtaataaata acgaaatcca tgatgtacac catcgcagtg
    11221 atacttctgg ttctctggct ccttggtctc gtgacgagct acacgctggg cggcttcatc
    11281 cacctgctgc tcgtcgtcgc ggtgatcatg gtgctcgtcc gcctcatcca gggcaggaac
    11341 cccatcgcgt aacgtagatc tccatgccgc ggggcagcaa ggccaagtac acggataagc
    11401 agaagcgcca agcccgccat atcgaggagg ggtacgagaa gcggggcgtt tccgaggacg
    11461 aggccgaagc ccgcgcctgg gccaccgtca ataagatgac cggtggcggc aagaagagcg
    11521 gctc
//
LOCUS       DASPKG010000049         5519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1435602, whole genome shotgun sequence.
ACCESSION   DASPKG010000049 DASPKG010000000
VERSION     DASPKG010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435602"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VEA36_01870"
     CDS             <1..479
                     /locus_tag="VEA36_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HYF29095.1"
                     /translation="VHNFVDGLIIAASYLVSIEVGIATTIAVVLHEIPQEIGDFGVLL
                     HAGYSKVKALFYNFVSALFAVLGAGVALLLGGATEAFTMWLVPLAAGGFIYIALTDLI
                     PELHKTTSVRHSLIQLVAVMAGVLAMVGLLAIEPEEHAHAGGIEVHELGEAEHGHE"
     gene            complement(506..1177)
                     /locus_tag="VEA36_01875"
     CDS             complement(506..1177)
                     /locus_tag="VEA36_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29096.1"
                     /translation="MQQLIAGVVLIIVVGAGAFLYRNMMERPAAPTDNEPVACTADAK
                     LCPDGSAVGRQGPNCEFSACPPPNVEVAEARIGFVLPAGYTQTMRGTATQETLRIFTK
                     PTLSSSVQHSISVKRYPVPAGKTAEQVILDNTRYQPADMNAENFSRFTTKTIGGNTFR
                     VTVIERFEAIIESSYFLARGSEVLRFDIVEHDVTNWMEPNLVVSNLPEHKALETMLAS
                     LKLYE"
     gene            complement(1224..1622)
                     /gene="mscL"
                     /locus_tag="VEA36_01880"
     CDS             complement(1224..1622)
                     /gene="mscL"
                     /locus_tag="VEA36_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HYF29097.1"
                     /translation="MKSFIKEFKEFALRGNAIDLAIGVVIGAAFNSIVTSLVTNVLTP
                     PLSLLTRGINFGDLAIAVPNTDAQIQYGLFIQAVISFVITALALFLLVKALNRLAAAA
                     RREEEQGKAEPAQKSAELVVLEEIRDSLKK"
     gene            complement(1627..1702)
                     /locus_tag="VEA36_01885"
     tRNA            complement(1627..1702)
                     /locus_tag="VEA36_01885"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1667..1669),aa:His,seq:gtg)
     gene            complement(1994..2374)
                     /locus_tag="VEA36_01890"
     CDS             complement(1994..2374)
                     /locus_tag="VEA36_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29098.1"
                     /translation="MDSAVLAEPRQLRLSFPDMESRHTRLVYVEGPVLAVDHQKKWFS
                     SKVMLTIGLSEADRLEINAARSSVGLDLIPEDTTSIRIELEPGKKLKLGRAGALRATL
                     GFNTWEKEHYLGFDRAGVVSVSPA"
     gene            2650..2877
                     /locus_tag="VEA36_01895"
     CDS             2650..2877
                     /locus_tag="VEA36_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29099.1"
                     /translation="MDKNPFDFLPPRNYWAEHLQHLGGRLSAAQSLMDDVINSFDELE
                     DAFRRELERRDQKADSGPFEALKQAGESRLR"
     gene            complement(2950..3034)
                     /locus_tag="VEA36_01900"
     tRNA            complement(2950..3034)
                     /locus_tag="VEA36_01900"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2998..3000),aa:Leu,seq:gag)
     gene            complement(3109..3636)
                     /gene="rplS"
                     /locus_tag="VEA36_01905"
     CDS             complement(3109..3636)
                     /gene="rplS"
                     /locus_tag="VEA36_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HYF29100.1"
                     /translation="MQNVITPVRVEERASLGIRPGDTVRVHQKVIEKGKTRLQTFEGL
                     VLAVKHGTEAGGTFTVRATMSGIGVEKIFPLYSPIIEKIEIVRRSKVRRAKLYFIREK
                     VAREVRRQLRNMRMVNISTDDFKEAEKVEEPMEADAVVVDEAPIEAAEPETPEEVAAA
                     EEAEAEAPAEEEKAA"
     gene            complement(3686..4294)
                     /locus_tag="VEA36_01910"
     CDS             complement(3686..4294)
                     /locus_tag="VEA36_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29101.1"
                     /translation="MSLGVKEFDPSEVLTAFRRLDRYTKQEWNAISRRDGRARAAYQI
                     CRTLKKLEQRSRWFRELSELRIDPGYRELYAQLQATLGLTLHLAALTELSEADAYRLG
                     VQGWPFYEHDPKRVEERYQGMLRSKRFETQVAALAAWVATGILRLEQAYCGQRLLLGG
                     VREVVCGLLTTLRDMAGDLGYCANPYLEQCIKAAANAARRSP"
     gene            complement(4295..5050)
                     /locus_tag="VEA36_01915"
     CDS             complement(4295..5050)
                     /locus_tag="VEA36_01915"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HYF29102.1"
                     /translation="MELPILYEDADIVAVAKPAGVMTHPDGHTKEETVSDWFARRYPD
                     VVHVGETQRLPDGTEIARPGIVHRLDRETSGVLVLAKTPEAHAHLKAAFKNREAKKLY
                     LAFTYGVPKERKGTIEFSIGRSRKDFRLRSAQPKAKGTLREAVTRYEVAGDIGTHALL
                     KVMPETGRTHQIRVHLKAIHHPVVCDSLYAPKQPCDLGFSRLGLHAYSLDIPLPSGEQ
                     RTFVAPIPKDLTEAFAKFGDAAYLLEGHNRGEG"
     gene            complement(5068..>5519)
                     /gene="uppS"
                     /locus_tag="VEA36_01920"
     CDS             complement(5068..>5519)
                     /gene="uppS"
                     /locus_tag="VEA36_01920"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HYF29103.1"
                     /translation="EAFHRLGERAKREGVIMRFIGQRERMPEDLQACIRASEEDTKGG
                     SEGVLTIALSYGGRAEIVAAVNRLLAEGADAVDEERFAAALWSAGTSDPDLIIRTGGD
                     QRLSNFLTWQSVYSELFFLDTPFPALARQEFDAVLVEFAARERRRGK"
BASE COUNT         1015 a         1737 c         1668 g         1099 t
ORIGIN      
        1 gcgtccacaa cttcgtcgac ggcctcatca tcgcggcgag ctacctcgtc tcgatcgagg
       61 tcggcatcgc gaccaccatc gcggtcgtcc tccacgagat cccgcaggaa atcggggact
      121 tcggcgtgct cctgcatgcc ggatacagca aggtgaaggc gctcttctat aacttcgtct
      181 ccgccttgtt cgcggtgctc ggtgcaggcg tcgcgcttct ccttggcggt gcgaccgagg
      241 ccttcacgat gtggcttgtc ccgcttgccg ccggcgggtt catctacatc gcgctcaccg
      301 acctcattcc cgagctccat aagacgactt cggtgcgcca ttccctgatc cagctggtcg
      361 ccgtcatggc aggcgtcctc gcgatggtcg ggctgcttgc tatcgagccc gaagagcacg
      421 cgcacgccgg gggtatcgag gtccacgagc tcggcgaagc cgagcacgga cacgaatgaa
      481 gatggcccca ttcgggacca tctcgctatt cgtagagctt caggctcgcg agcatcgtct
      541 cgagcgcctt atgctcgggc aggtttgaga cgacgaggtt cggctccatc cagttcgtga
      601 cgtcatgctc gacgatgtcg aagcggagta cctcgctacc gcgcgcaagg aagtacgagc
      661 tctcgatgat tgcctcgaag cgctcgatga ctgtcacgcg gaaggtgttg ccgccgatcg
      721 tcttcgtggt gaagcgagag aagttctccg cgttcatatc cgccggctgg taccgcgtgt
      781 tgtcgaggat gacctgctcc gcggtcttcc ctgccgggac cgggtaccgt ttcaccgaga
      841 tcgagtgctg gaccgaggac gagagcgtcg gcttcgtaaa gatgcgcagc gtctcctgcg
      901 tcgcggtgcc ccgcatggtc tgggtgtacc ctgccgggag cacgaatccg atgcgcgcct
      961 ccgccacctc gacgttcggt ggggggcagg cgctaaattc gcagttgggg ccctggcgcc
     1021 cgaccgcgct gccgtcaggg cagagcttgg cgtccgcggt gcaggcaacc ggttcgttat
     1081 cggtcggcgc tgcggggcgc tccatcatgt tgcgatacag gaaggcaccc gccccgacca
     1141 cgatgatgag cacgacgccc gcgatgagtt gctgcatgga aagcataggt agtggttaag
     1201 acggatacgg tacaggttcc tttctatttc ttcaatgaat cccggatctc ctcgagcacc
     1261 acgagctcgg ccgatttctg ggcgggctcg gccttgccct gctcctcttc ccggcgcgcc
     1321 gcagctgcga gccggttcag cgccttcacg agcaggaaga gcgcgagggc ggtgatgacg
     1381 aagctgatca ctgcctggat gaagagtccg tactggatct gggcgtcggt gttcgggacg
     1441 gcgatggcga gatccccgaa gttgatccca cgcgtaagca gcgagagcgg cggggtgagc
     1501 acattggtta cgagcgaggt gacgatgctg ttgaacgctg cgccgatcac tacgccgatc
     1561 gcaagatcga tcgcgttgcc gcgcagggca aattccttga attctttgat gaaagatttc
     1621 atatcttggg gcgacgtgtg ggactcgaac ccacgaccgc tcggaccaca accgagagct
     1681 ctaccagctg agctaacgcc gccatgtacg tgcgttatag cacaaatgca aaagcgccta
     1741 aaggcgctcg tgcattggtg caccgtgttg gaccgtactt cgaactcctg acggtgggtc
     1801 agaaaccgac ctcttgtcac atggtgcacc gtgttggggg gacatcgaac acctgactta
     1861 ccgtcagaaa ctgacgtctc ggtctctgat agatcgcatc ggaattacct gaatccaaaa
     1921 atactcgacc cccgcccgag cgcattattt gctcgaagcg ggggtgtggg atggcatctc
     1981 tggatgctgg tgcctaagcg gggctgacgc tgacgacccc agctcgatcg aaaccgagat
     2041 agtgctcctt ctcccaggta ttgaaaccga gggttgcgcg tagcgcacca gcccgaccca
     2101 gcttcaactt cttgccaggc tccagctcga tgcggatgct cgtggtgtcc tctgggatga
     2161 gatcgagccc tacggacgag cgcgccgcat tgatctccag ccgatcagcc tcagagaggc
     2221 cgatagtgag catgaccttg ctgctgaacc acttcttctg gtggtccaca gcaagaactg
     2281 ggccttcgac gtagacgagc cgagtgtgcc gactctccat atcgggaaaa gaaagccgga
     2341 gctgccgggg ctcggcgagc acagcactat ccatgatgtt ttctccaagc tacctggcca
     2401 ctcgtggatg aatgggcagg taattagaga aaacgatgaa aagaactgct tcgagtgcac
     2461 cgtaccacga actcatcaaa tcgcaaggag tgttcggaca aagtcaacga tatcgccaag
     2521 attcgggatt tgtagattcg gtactcatga aatgtcacaa agtgacgccg tgcgctcggt
     2581 gaccgcccac cctaccattc tctcgggcgc gcgcctgatc attgaaagct caataagcac
     2641 acaaccaata tggacaagaa tccattcgac ttcctaccgc cgcgcaatta ctgggcagag
     2701 catctgcaac accttggggg acgcctctcg gcagcgcaat cgctcatgga cgacgtcatc
     2761 aactccttcg acgagttgga ggacgcgttc cgaagagagc tggagcgcag ggaccagaaa
     2821 gcggacagtg gtccgtttga ggccctaaag caggctgggg aaagtcgctt gaggtaaaac
     2881 gcctcaacgc ccgggatctg tcccgggcgc caaggatttg aatacacatt tgactctttt
     2941 tcgaatcttt ggtgcccagg agaggactcg aacctccacg ccttgcggca catccacctc
     3001 aaggatgcat gtataccaat ttcatcacct gggcaaacgt ttccgctcca tacggaagcg
     3061 gcccgcttac tatagcgggc cgcttccgta tttacaaatg caggaccgtt aggccgcctt
     3121 ctcctcctcg gcgggtgctt ctgcctccgc ttcctcggca gccgcgacct cctccggggt
     3181 ctcgggctcc gcagcctcga tcggtgcctc gtccacgacg acagcatctg cttccatcgg
     3241 ttcctccacc ttctccgctt ccttgaagtc atcggtggag atgttcacca tccgcatgtt
     3301 acggagctga cggcgcacct cgcgggcgac cttctcgcgg atgaagtaga gcttggcgcg
     3361 gcgcaccttc gagcggcgca cgatctcgat cttctcgatg atggggctgt agagcgggaa
     3421 gatcttctcc accccgatcc cggacatcgt ggcgcggacc gtgaaggtgc cgccggcctc
     3481 cgtgccgtgc tttaccgcaa ggacgaggcc ctcgaaggtc tggaggcggg tcttcccctt
     3541 ctcgatcacc ttctgatgga cacggacggt gtcgcccggg cgaatgccga gcgaggcgcg
     3601 ctcctcgacg cgtacgggag tgatgacgtt ttgcatgacc ggggtactgt accacagcct
     3661 cttttctacg caaaacggcg gggccctagg ggctccgccg cgccgcgttc gcggctgcct
     3721 taatgcactg ttcgaggtag ggattggcac agtagccgag gtcccccgcc atatcgcgca
     3781 gcgtcgtgag tagtccgcac acgacctcgc gcacgccgcc gagcaggagg cgttggccgc
     3841 agtatgcctg ctcgagccgg agaatccccg tggcgaccca ggccgcaagc gcggctacct
     3901 gcgtctcaaa acgcttgctc cggagcatgc cctgatagcg ttcttcaacg cgcttgggat
     3961 cgtgctcgta gaacggccag ccctgcaccc caagccggta cgcatccgct tcgctgagct
     4021 cggtgagcgc ggcgagatgc agcgtgagac caagggtcgc ctgcagttgc gcatacagct
     4081 cccggtatcc tggatcgatg cgcagctcgg agagctcgcg gaaccagcgg ctccgctgct
     4141 cgagcttctt cagcgtccga cagatctggt aggctgcgcg tgcccggcca tcgcgccggc
     4201 tgatcgcgtt ccactcctgc ttggtgtacc ggtctaagcg ccggaaggca gtgagcactt
     4261 ccgaaggatc gaattctttt acaccgaggc tcatctagcc ctctcctctg ttgtgtccct
     4321 cgagaagata cgccgcgtcc ccaaacttcg caaacgcttc cgtaagatcc ttcggtatcg
     4381 gtgcgacgaa cgtccgctgt tctccggacg gaagcgggat gtcgagcgag tatgcatgga
     4441 ggccgagccg cgagaagccg aggtcgcacg gctgcttcgg cgcgtatagg gaatcgcaga
     4501 cgaccggatg atggatcgcc ttcaagtgca cgcggatctg gtgcgtcctg ccggtctcgg
     4561 gcatgacctt cagtaaggca tgcgtgccga tatcccccgc cacctcgtag cgcgtgacgg
     4621 cctcgcggag cgtgcccttg gctttgggct gcgcgctgcg caggcggaag tccttgcggg
     4681 accgcccgat cgagaactct atggttccct tccgctcctt cggcacgccg tacgtgaacg
     4741 caagatacag cttcttcgct tcccgattct tgaacgccgc tttcaggtgc gcgtgcgctt
     4801 ccggggtctt cgcaagcacg agcacccccg aggtctcgcg atcgagccga tgcacgatgc
     4861 cgggccgcgc gatctccgtc ccgtccggga gccgctgggt ctcgccgaca tggacgacat
     4921 cgggatagcg gcgcgcgaac cagtcgctta cggtttcctc cttcgtatgt ccgtccggat
     4981 gcgtcatgac gcccgcgggc ttggcgaccg cgacgatatc ggcgtcctcg tagaggatgg
     5041 gcaattccat tgcccctacc ctatcaccta tttcccgcgc cggcgttcgc gcgcggcgaa
     5101 ctcgacgagc accgcatcga actcctggcg cgcgagcgca gggaacggcg tatcgaggaa
     5161 gaagagctcg ctatataccg actgccaggt aaggaaattg gagagccgct gatcgcctcc
     5221 ggtgcggatg atgaggtcgg gatcgctcgt ccctgcgctc cagagcgccg ctgcgaagcg
     5281 ctcctcatcg accgcatccg cgccctcagc gagaagccgg ttgacggcag cgacgatctc
     5341 cgcacggccg ccgtaggaaa gcgcgatcgt gagcacgccc tcgcttccgc cctttgtgtc
     5401 ctcctcggac gccctgatgc acgcctgcag gtcttccggc atgcgttcac gctggccgat
     5461 gaagcgcatg atcactccct cgcgcttcgc ccgttccccg agcctgtgga atgcctcgc
//
LOCUS       DASPKG010000050         3839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1978326, whole genome shotgun sequence.
ACCESSION   DASPKG010000050 DASPKG010000000
VERSION     DASPKG010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978326"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VEA36_01925"
     CDS             complement(<1..611)
                     /locus_tag="VEA36_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29104.1"
                     /translation="MTANVAGQEWMGWKERASAARSVLARARAHIIGYQEQSPESLAV
                     FREAKPGHDFYPGEPFADILIQTVSMDWSRFRRLDCGTFWLSTSGKPEKSAPDSCAPR
                     GTSWQLLWDLQAKRRVMHINSHLDNVSARGRRAETLINLRFIGTVDPEIPIVYTADSN
                     VSIASPNERWREPDMQEPYTFMQGAGFRDAWVEAHPGEPRPCT"
     gene            complement(691..1098)
                     /locus_tag="VEA36_01930"
     CDS             complement(691..1098)
                     /locus_tag="VEA36_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYF29105.1"
                     /translation="MEKITVESVINAPVEKVWRDYTDPEAVKAWNAASDDWHTTQAAN
                     DLREGGSFLYRMEAKDGSAGFDFGGVYDAVVPKERIAYTMDDGRKVETLFASESDGTR
                     ITVSFDPETENTPELQRAGWQAILDNFKKYVEA"
     gene            complement(1110..2207)
                     /gene="ychF"
                     /locus_tag="VEA36_01935"
     CDS             complement(1110..2207)
                     /gene="ychF"
                     /locus_tag="VEA36_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006909031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HYF29106.1"
                     /translation="MLKIGIVGLPNVGKSTLFNALTKAAVPAENYPFCTIDPSVGVVG
                     VPDERLEKLAELSKSEKVIPAAIEFVDIAGLVKGASEGEGLGNQFLSNIREVDAILEM
                     VRFFDDPDVTHVHGDVDPVRDIEVINLELILADRDQVTKRREKLVRDIRAGVKEAIAE
                     DAVLASVEQALGTGTLARNAGLTADEARLIRSLNLLTMKPILYSFNGKSGGHNLSEVN
                     DGRADAAYRLVESLGSTWVEVDARTEHELNDVADEDKQMFRQELGVQVDGLEALIRGA
                     YETLGLITFFTTGPKESRAWTVKRGATAPEAGAAIHNDFKDKFIRAEVIAWNALLADG
                     SWSAARDKGDIRTEGKEYIVKDGDVMVFLHS"
     gene            complement(2372..2518)
                     /locus_tag="VEA36_01940"
     CDS             complement(2372..2518)
                     /locus_tag="VEA36_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29107.1"
                     /translation="MTDMTYDRPEKGEIRYTTEDEIRFVNCLAATKRREFYEYAKTMY
                     DRDR"
     gene            complement(2576..3157)
                     /locus_tag="VEA36_01945"
     CDS             complement(2576..3157)
                     /locus_tag="VEA36_01945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HYF29108.1"
                     /translation="MAKRIFILLGHPDAGPEPLSRQLADAYEAGAREAGHEVRRMNIF
                     ELSFDANLHKGYRAIQELEPDLKAVQENLSWCQHFVLVYPNWWGSMPGLLKGMWDRMF
                     LPRFAFRMWKNRLGWDALLKGRTARVIITCGNPVILDHLAFGDFTDTIKRSLLQFAGF
                     RTWVTAVGRSEHLSEWSKNGWLKMVKGLGRRGR"
     gene            complement(3158..3388)
                     /locus_tag="VEA36_01950"
     CDS             complement(3158..3388)
                     /locus_tag="VEA36_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29109.1"
                     /translation="MGMQDFLLRQAMKWKLKDMPEAQREQIMTLVQKDPELFKKIGEE
                     VEKRTKGGEPQMKATMEVMKKYRSELAALMQR"
     gene            complement(3424..>3839)
                     /locus_tag="VEA36_01955"
     CDS             complement(3424..>3839)
                     /locus_tag="VEA36_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29110.1"
                     /translation="GIMNALVSIPARKLLGSAHEARFERIMDRTRHRNFPDWFYGVRR
                     ASEDQDLRQGFDFVAELDVVEVTLQIKSSVAGREHHNEVQHRKCGTGRYMVPTVIVCE
                     FFSDETVRAQTLRILDEERSRFFRGPDRYRRETRR"
BASE COUNT          662 a         1283 c         1126 g          768 t
ORIGIN      
        1 aaggtgcagg gccggggctc tcccggatgc gcctccaccc aggcgtcccg gaagcccgcg
       61 ccctgcatga acgtgtaggg ctcctgcata tccggctccc gccaccgctc gttcgggctc
      121 gcgatgctca cgttcgagtc cgcggtgtac acgatcggga tctcggggtc gaccgttccg
      181 atgaacctga gattgatcag cgtctcggcc cggcgcccgc gagcgctcac gttgtccagg
      241 tgcgagttga tatgcatcac acgccgcttc gcctgcaggt cccagaggag ctgccagctc
      301 gtcccgcgcg gcgcgcagga atccggcgcg ctcttctccg gcttcccgct cgtcgagagc
      361 cagaacgtcc cgcaatcgag acgcctgaac ctgctccagt ccatcgagac ggtctggatg
      421 agaatgtccg cgaacggttc gccgggatag aagtcatggc cgggcttcgc ctcccggaag
      481 accgcgaggc tctccgggct ctgctcctgg tatccgatga tgtgcgcgcg ggcccgggcg
      541 agtaccgagc gggcagcaga cgcacgttcc ttccacccca tccactcttg gcccgccacg
      601 ttggcggtca ttacggtaaa cagcacggcc gcctcctgcg ggttgcatct cgcgccacgc
      661 tacggccgca gccgtgaagg ggccatgagg ctaggcctcg acgtacttct tgaagttgtc
      721 gaggatggcc tgccagcctg cgcgctgcag ctcgggcgtg ttctcggtct cgggatcgaa
      781 ggagacggtg atgcgggtgc cgtcgctctc tgacgcgaac agggtctcca ctttccggcc
      841 atcgtccatg gtgtacgcga tgcgctcctt cggcacgacg gcgtcataga caccgccaaa
      901 gtcgaatccc gcgctcccgt ccttcgcctc catgcggtac aggaagctgc cgccctcgcg
      961 caggtcgttc gcggcctgcg tggtatgcca gtcatccgat gcggcgttcc aggcctttac
     1021 cgcttcgggg tccgtgtaat ctcgccagac cttctcgacc ggtgcattga taacgctctc
     1081 gacagtgatc ttctccatac gtaggggtat tagctatgta aaaacaccat cacgtccccg
     1141 tccttcacga tgtactcctt gccctcggtg cggatatcgc ccttatcgcg ggcggcgctc
     1201 caggatccat cggcaagcaa ggcattccat gcgatgactt cagcgcggat gaacttgtcc
     1261 ttgaaatcgt tgtggatggc ggccccagct tcaggagcgg tggcaccccg cttcaccgtc
     1321 cacgcacggc tctccttcgg gcccgtcgtg aagaaggtga tgagccctaa ggtctcgtac
     1381 gcgccgcgga tcagggcctc gagcccgtcc acctgcacgc cgagctcctg ccggaacatc
     1441 tgcttgtcct cgtccgcgac atcattcagt tcatgctccg tgcgggcatc cacctccacc
     1501 caggtggacc cgagggactc cacgagcctg tatgcggcgt ccgcccgacc gtcgttcacc
     1561 tccgaaaggt tgtgcccgcc gctcttcccg ttgaaggaat agaggatggg cttcatggtg
     1621 aggaggttga gcgagcggat gagccttgcc tcgtctgcgg tgagtcccgc atttcgtgcg
     1681 agcgttcccg taccgagcgc ctgctcgacc gaggcaagga ctgcgtcctc ggcgatcgcc
     1741 tccttcaccc cggcgcgaat gtcgcgcacg agcttctccc ggcgcttcgt gacctggtcc
     1801 cggtccgcga ggatgagctc gagattgatc acctcgatgt cccggaccgg atccacgtcc
     1861 ccatgcacgt gcgtgacgtc cggatcgtcg aagaaccgca ccatctcgag gatggcgtcc
     1921 acctcccgga tattggagag gaattggttc ccgagcccct cgccctcgga tgcgcccttc
     1981 acgagtccgg cgatgtccac gaactctatg gctgccggga tgaccttctc tgacttcgag
     2041 agctcggcga gcttctcaag ccgctcgtcc ggcaccccca ccacccccac cgatggatcg
     2101 atggtgcaga agggatagtt ctcggcaggg accgccgcct tggtgagcgc gttgaagagg
     2161 gtggacttcc cgacgttcgg gagcccaacg atgccgatct tgagcatagt tcctacctta
     2221 cgggttcacc tgtaaaaaga aaaggcggcg aaagtgccgc cgccagttct gcggggccga
     2281 aggcggccta cgccgcgatc ggaagcctgg gctgctccgc atcgaggagc cgacggacgc
     2341 gctggaagac cttgtgtcga tcgatgagcc ctcagcggtc acggtcgtac atggtcttgg
     2401 cgtactcgta gaactcgcgc cgcttcgtcg ccgccaagca gttgacgaac cggatctcgt
     2461 cctcggtggt gtagcggatc tcaccctttt cggggcggtc gtacgtcatg tcagtcatgg
     2521 gcagtacccc tttcctgagg taactaaagc acgtacacac cctgcaaaca accagttatc
     2581 tacccctccg cccgagcccc ttcaccatct tcagccaccc attcttggac cactccgaga
     2641 gatgctcgga ccgccccacg gccgttaccc aggtccggaa tcccgcgaac tgcagcagcg
     2701 accgcttgat cgtatccgtg aagtcaccga aggcgaggtg atcgaggatg accgggttgc
     2761 cgcaggtgat gatcacccgc gcggtgcgac ccttcagcag cgcatcccag cccaatcggt
     2821 tcttccacat ccggaaggca aagcgcggca ggaacatgcg atcccacatg cccttgagta
     2881 ggcccggcat cgagccccac cagttcgggt acacgagcac gaagtgctgg caccacgaga
     2941 ggttctcctg cacggccttc aggtccggct cgagctcctg gatggcgcgg taccctttat
     3001 ggaggttcgc atcgaaggag agctcgaaga tgttcatccg ccgcacctcg tggccggctt
     3061 ctcgggcacc agcctcatac gcatcagcta actgacgcga gaggggttcg ggacccgcat
     3121 ccggatgccc gagcaggatg aagatgcgct tagccattta cctctgcatc agcgccgcga
     3181 gctcgctgcg gtacttcttc atcacctcca tcgtcgcctt catttgcggc tcgccgccct
     3241 tcgtgcgctt ctccacctct tcaccgatct tcttgaagag ctccggatct ttctgcacga
     3301 gcgtcatgat ctgctcgcgc tgcgcctccg gcatgtcctt cagcttccat ttcatggcct
     3361 gccgcagcag gaaatcttgc atacccatat gccgctagta taccaaacgt gttgagaccc
     3421 gcctcatcgg cgggtctccc tccggtaacg gtccggaccc ctaaagaagc gtgatcgttc
     3481 ctcatcaagg atgcggagcg tctgcgccct gaccgtttcg tccgaaaaga actcgcagac
     3541 gatcacggtt ggtaccatgt agcggccagt gccgcatttg cggtgctgca cttcgttgtg
     3601 atgctcgcgc cctgcgacgg acgacttgat ttgaagcgtg acctcgacta cgtcgagctc
     3661 ggcgacaaaa tcgaagccct ggcggaggtc ttgatcctcg gaagctcgac ggacgccata
     3721 gaaccagtcc gggaagttcc ggtggcgggt ccgatccatg atccgctcga atcgagcctc
     3781 gtgcgcgctt ccgagcagct tgcgcgcggg tatgctgacg agtgcgttca tgatcccat
//
LOCUS       DASPKG010000051         9698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1166152, whole genome shotgun sequence.
ACCESSION   DASPKG010000051 DASPKG010000000
VERSION     DASPKG010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9698)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9698
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166152"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2043
                     /locus_tag="VEA36_01960"
     CDS             <1..2043
                     /locus_tag="VEA36_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HYF29111.1"
                     /translation="TRSRTSGCGSSSRKRPALRYHPPSMRTAVLANGSLLVALDEFGR
                     VADLYFPHVGLEAHAREGTPGRIGVYVDGMLSWLGEDGRWDISVAHEEEALAARVVAR
                     NEHLGVELAFTDVVYNESPIFIRRVVVKNLTDTSREIKVYLGQELEIYRSPVGDSAYY
                     DPADGTIIHYKGQRVFLVGGTIDDAPLSDYAIGLTGYAEKEGTFRDAEDGALSKNSIE
                     HGPVDSVVGFYGDFDAGAEKVIRYWLCAGDSLARAKELHAYLLKKKPDHLVRTSGSYW
                     HAWVNKYTWSFHGMPPEQIALFKKSLMLVRAHVDDGGGIIASADSDTLQGGKDTYAYV
                     WPRDAAYAAMTLDRAGDPNVARRFFSFCEKTIRDEGYFMHKYLPDGSLGSSWHPWIKD
                     GKTQLPIQEDETALVIYALNEHYQHSKDLEFLESLFNPLVVKAADFMVSYRDQKTGLP
                     LPSYDLWEEQRGVSTFTASAVYGALMAAVEMSTLLGKEKSAKKYRDAAESVKAGILAH
                     LYDEGTGTFAKLLDPDTGVADRTLDTSSVYGVFAFGVLPADDPRLAKAFDAVFAALSR
                     GVECGGIGRYEHDEYYRTGAGSNPWFVTTLWYAEYLTARARSEEELEAVRNIFTFVVE
                     RALPSGVLSEQLDAQTGAQLSVAPLTWSHSAYVSAVLKYLDKLEALGVCAACNPTP"
     gene            2066..3025
                     /locus_tag="VEA36_01965"
     CDS             2066..3025
                     /locus_tag="VEA36_01965"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HYF29112.1"
                     /translation="MEEQLLSLPEQFSWEPAYDGPAGERFLVCGMGGSHLAASLLLVH
                     DPALPIRIHRDYGLPAIPDAERAGTLVIVSSYSGNTEEELDAAEAALEAGFPTVAITT
                     GGRLAEFAVERGLPLIRLPDSDVEPRMAIGHALLALAAVIGGQDLVEEVRAIGSSLDP
                     EAQQEMGSEVGSALTERIPLIYATTEHEIVARMWKIFLNETAKVPAFSYSVPEFCHNE
                     LSGFDTAPATEILSERMLPMFLTSDADHPRNTLRMEIARDLLNERGIDTIAVPLEGET
                     ALGRALSGIATGVWAALALAAAYDVPDAKTPLIAEFKERMQDV"
     gene            3022..3819
                     /locus_tag="VEA36_01970"
     CDS             3022..3819
                     /locus_tag="VEA36_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYF29113.1"
                     /translation="MILAADIGGTNMRVAIGRPDGTILRSEKFDTPETGEMGAERLIE
                     AMRQLRGEEEVSAVVVGIAGVLHKDRKSLRWSPHLEGWVGYDLHGALSQALSLPVTLE
                     NDAALGALGEAVAGAGAGSAIVAYVAVGTGIGGARIVDGKIDRSAFGFEIGHQYVSGD
                     VELEEMVSGTAVERSHGVPAKDIMDMDIWREYAKRFAYGLYNTLLHWSPDTVVLSGSL
                     MRESGMHVEDIRAELERINRAIPSLPDIRHGKLEYPGLVGALILTDS"
     gene            3847..4836
                     /locus_tag="VEA36_01975"
     CDS             3847..4836
                     /locus_tag="VEA36_01975"
                     /inference="COORDINATES: protein motif:HMM:NF016235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HYF29114.1"
                     /translation="MRYFLVPTVVSALIMLGILVWLGPVAFVTAVLLTILEVTLSFDN
                     AVVNARVLMRMSEKWQQRFLTWGILIAVFGTRLVLPILIVSVGVWASPWAVAQLAAFD
                     PETYGHLLEEAKHGIYAFGASFLIMVSLKYFFNEAKETHWIQVIERQLSKLGRIEAIE
                     IGVALLTILAFSFLVPDEQGVILAAGIVGIVLFIVMEGLASSFSEGVKGATASGLALF
                     MYLNVLDSAFSLDSVVGAFALTTSIPIIVAGLGIGAWYVRSMTIYFVRKNTLDTLVYL
                     EHGAHWAIFGLAVSMFAGLLFEVPEIVTGGIGLAFVAAAYYSSLRFRKMNRGV"
     gene            4882..5253
                     /locus_tag="VEA36_01980"
     CDS             4882..5253
                     /locus_tag="VEA36_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29115.1"
                     /translation="MPCCINNRRRPSVKLSILAVAAALSLSGAPAFAQLAAISSSPQP
                     RARPVTINAADCSRLYPLQVQNYALQSDAAHLEFKMEKSPSVTQIMRDNLAVTKASLA
                     RSRRHLAQLENYCRTYHRPWR"
     gene            5338..6981
                     /locus_tag="VEA36_01985"
     CDS             5338..6981
                     /locus_tag="VEA36_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYF29116.1"
                     /translation="MSLKETLAASLTGEVDDDALTRDKMSRDTSVFQRTPSLVVYPKD
                     TEDVSKLVKAIAKEPGVSVTARSAGTDMSGGPLTDSVVAVFTKYMNKVHEVADGYAIA
                     EPGVYYRDFEKETRKVHNQLLPSFPASRELCALGGIVNNDSGGERTLEYGKTRDYVEE
                     LEVVLSDGTITTFGELGPAELAEKKELPGLEGEIYRRMSELLIDNREVIAAAKPKVSK
                     NSAGYALWDVMDMEDGTFNLAKLITGAQGTLALTTKMKLRLVREQGYRSMLVIFLKDI
                     DHLPEIVHKVLPYNPESFESYDDQTFGLAVRFLPQMLSQMGLGRAIRLGLSFLPEVGM
                     AMRGGVPKLILMAEFSEDSREEALKKAREAEAAIKHLKLQTSIKKNEQAAEKYWVVRR
                     ESFALLRKNSHGLYASPFIDDFVVPVDSYPEFMPELNALLAEYKDKFVYTIAGHIGNG
                     NFHIIPLMDLNKEESRKIILELSPRVYELVIKYGGTTTGEHNDGIIRTPYLPMLFGEK
                     MVSLFEETKRIFDPNNIFNPGKKVGGTFADIEKSILKHI"
     gene            7001..7762
                     /locus_tag="VEA36_01990"
     CDS             7001..7762
                     /locus_tag="VEA36_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009916 - alternative oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative oxidase"
                     /protein_id="HYF29117.1"
                     /translation="MSDEHRAHEELSKQLNDPKLRHEYAKRYDHYQVGWLARFLGWLL
                     VSSGDLVYGTKPSYAKFKAIEVVARVPYQSWEVAAYTLLSAFYRNEKRAIELAKTSAF
                     SRLAQDNETLHVIVLSQIVCKLKQQRFFRHTLVPFLFALFYFIAVYFLYLVYRKAALE
                     LNYVFENHAYHQYAEFLERNEAVLKHRICMYEFLSFYGRKVVTEYEFFESVRNDELIH
                     RNTTIRELEARGYFCMPKMPEEDNPQGDAANLARS"
     gene            7868..8230
                     /locus_tag="VEA36_01995"
     CDS             7868..8230
                     /locus_tag="VEA36_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HYF29118.1"
                     /translation="MASNKLYVGGIPYRTTEDELRTAFEEAGTVTSVSIISDRMTGRS
                     RGFGFVEMADEAQAQAAIDRWDGKEMDGRMLSVSMARPQGDRPPREGGGGGRGGFGGG
                     NRGGFGGGNDRGGYGGGY"
     gene            complement(8276..8734)
                     /locus_tag="VEA36_02000"
     CDS             complement(8276..8734)
                     /locus_tag="VEA36_02000"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HYF29119.1"
                     /translation="MKAFWNIAFSVFFLFLLVNGIAWLAMTGKFDRPVSLGDFVLMAL
                     ATWRLIRLFTYDAITAFVRDWFANAAPHTFLGTLKTLINCPWCIGLWFSFIVVFFYFA
                     TIYAWPVILILALAAIGSFLQILSNLVGWSAEAKKRSVLGSGPGQGGTCG"
     gene            complement(8788..9096)
                     /locus_tag="VEA36_02005"
     CDS             complement(8788..9096)
                     /locus_tag="VEA36_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29120.1"
                     /translation="MTNDTSSVAKKPKLAPDAVCAGLSHDVHAILSVCIHQFTSKEEL
                     EECFDVDAVISGRSLKPKVRYEHRITPASWSSRKVPVGTDRPYTAAQKGSFIPRGYCA
                     "
     gene            9125..>9698
                     /locus_tag="VEA36_02010"
     CDS             9125..>9698
                     /locus_tag="VEA36_02010"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYF29121.1"
                     /translation="MSIPSAEDVKALIRDKYDGVESSALAGDIARLAAGEPLAYVIGW
                     VPFLGLRIDLSSRPLIPRPETEYWTEVLIGELKKRRVPRVLDLCAGSGAIGLAVAKHV
                     PGATVSFGELELAHTAQIRKNLELNGVGGTVRRGNLFAPFFDERFDVIATNPPYVPEG
                     RTLEESVAAHEPPVALFAGPAGLSVIERIAR"
BASE COUNT         1759 a         3003 c         3144 g         1792 t
ORIGIN      
        1 acgcgctcca ggacctccgg ctgcggctcg agctcgagga agcggcccgc gctgcggtat
       61 catccaccta gtatgcgcac cgcggtcctc gccaatggtt ccctgctcgt cgcgctcgac
      121 gagtttggtc gcgtcgccga tctctatttc ccgcacgtcg ggctcgaggc gcatgcgcgc
      181 gagggcacgc cggggcgcat cggggtgtac gtggacggca tgctctcctg gctcggggag
      241 gacggacgtt gggacatctc ggtggctcat gaggaagaag cgctcgcggc gagggtggtc
      301 gcccgcaacg agcatctcgg ggtggagctc gccttcacgg atgtggtcta caacgagagc
      361 cccatcttca tcaggcgcgt cgtcgtgaag aacctcaccg atacgtcccg ggagatcaag
      421 gtgtacctcg gacaggagct cgagatctac cggtcgcccg tcggcgactc cgcctactac
      481 gaccccgcgg acgggaccat catccactac aagggccagc gcgtgtttct ggtgggcggc
      541 acgatcgacg atgcgcccct ctcggactac gcgatcgggc tcacgggata cgccgagaag
      601 gagggcacct tccgggatgc ggaggacggc gcgctctcga agaactccat cgagcatggg
      661 ccggtggatt cggtggtcgg gttctacggg gactttgatg ccggcgccga gaaggtcatc
      721 cggtactggc tgtgcgccgg cgactcgctc gcccgcgcca aggagctcca cgcgtacctc
      781 ctcaagaaga agccggacca tctggtgcgc acctccggga gctactggca tgcctgggtg
      841 aacaagtaca cctggagctt ccacggcatg cccccggagc agatcgcgct cttcaagaag
      901 agcctgatgc tcgtacgggc gcatgtggac gacgggggcg ggatcatcgc gtcggcggat
      961 tcggatacgc tccagggcgg caaggatacc tacgcgtacg tgtggccgcg cgacgctgcc
     1021 tacgccgcga tgaccttgga ccgcgcgggg gaccccaacg tcgcgcgccg gttcttctcc
     1081 ttctgcgaga agaccatccg ggacgagggg tatttcatgc acaagtatct ccccgatggt
     1141 tccttgggat cctcctggca cccctggatc aaggacggga agacgcagct ccccatccaa
     1201 gaggacgaga ccgcgctcgt gatctatgcg ctcaacgagc actaccagca cagcaaggac
     1261 ctcgagttcc tcgagagcct cttcaatccg ctcgtggtga aggcggcgga cttcatggtg
     1321 tcctaccggg accagaagac ggggcttccg ctcccctcct acgacctctg ggaggaacag
     1381 cggggcgtct ccaccttcac cgcaagcgcg gtatacggag ccttgatggc cgccgttgag
     1441 atgtctacgc tcttaggcaa ggagaagagc gcgaagaagt accgggatgc ggccgaatcc
     1501 gtgaaggcgg gcatcctcgc gcacctctat gacgagggca cgggcacctt cgcgaagctg
     1561 cttgatcccg ataccggcgt cgcggaccgc acgctcgata cctcgagcgt ctatggcgtg
     1621 ttcgccttcg gcgtcctgcc ggcggacgat ccgcgcttag cgaaggcctt cgatgcggtc
     1681 ttcgccgcgc tctcgcgggg cgtcgagtgc ggagggattg gacggtacga gcatgacgag
     1741 tactaccgga ccggcgcagg cagcaatccc tggttcgtca cgacgctctg gtatgccgag
     1801 tacctcaccg cccgcgccag gagcgaggag gagctcgagg cggtgcgcaa tatcttcacc
     1861 ttcgtcgtcg agcgggcgct tccctccggg gtcctctcgg agcagctcga tgcccagacg
     1921 ggggcgcagc tctccgttgc cccgcttacc tggagccact ccgcatacgt gagcgcggtg
     1981 ctgaagtatt tggataagct cgaggcgctc ggggtctgcg cggcgtgcaa cccgacgccc
     2041 taagcctgga cgctatactt acgccatgga agagcagctg ctttcgctcc ccgagcaatt
     2101 ctcctgggag ccagcgtatg acggccccgc cggcgagcgg tttctggtct gcggcatggg
     2161 cgggtcgcac ttggctgcgt ccctgctctt agtgcacgat cccgcgcttc ccatccgcat
     2221 ccaccgggac tacggtctgc ccgccatccc cgatgcggag cgcgccggca cactcgtgat
     2281 cgtctcctcc tattccggca ataccgagga ggagctcgat gccgccgaag ctgcgctcga
     2341 agcagggttc ccgacggtcg cgatcaccac cggcggcagg ctcgccgagt tcgcggtcga
     2401 gcgcggcctc ccgctcatca ggctccctga ttctgacgtc gagccgcgca tggccatcgg
     2461 gcacgcgctc cttgcgcttg cagcggtcat cggcgggcag gatttggttg aggaggttcg
     2521 cgccatcggc tcctcgctcg atccggaggc gcagcaggag atgggcagcg aggtcggcag
     2581 cgcgcttacg gagcgcatcc ccctcatcta cgccacgacc gagcatgaga tcgtggcgcg
     2641 gatgtggaag atcttcctga acgagactgc gaaggtcccg gccttcagct actccgtgcc
     2701 tgagttctgc cacaacgagc tctcgggctt cgacaccgcg ccggcgaccg agatactttc
     2761 tgagcgcatg ctgcccatgt ttcttacgtc cgacgcggat catccccgca acacgctgcg
     2821 gatggagatc gcgcgggacc tcctgaacga gcgcgggatc gatacgatcg cggtgccgct
     2881 tgagggcgag accgcgctcg ggcgcgccct gtcgggcatc gcgaccgggg tgtgggcagc
     2941 gctcgcgctc gcggcagcgt atgatgtacc ggacgccaag acgccattaa tcgccgaatt
     3001 caaggaacgg atgcaggacg tatgatactt gccgctgaca tcgggggaac gaatatgcgg
     3061 gtcgcgatcg ggcggccgga cgggaccatc ctgcgctcgg agaagttcga tacgccggag
     3121 accggcgaga tgggcgccga gcggctcatc gaggcgatgc ggcagcttag gggcgaggag
     3181 gaggtctcgg cggtcgtcgt cgggatcgcg ggcgttttgc ataaagacag gaagtcgcta
     3241 cggtggtcgc cgcatcttga gggctgggtg ggctatgacc tgcatggggc gctttcgcag
     3301 gcgctctcgc tcccagtaac gctcgagaac gatgccgcct tgggcgcgct tggcgaggcg
     3361 gtcgcgggcg cgggcgccgg gagcgcgatc gtcgcgtatg tggcggtcgg gacgggcatc
     3421 ggcggcgcgc gcatcgtcga tgggaagatc gatcggagcg ccttcgggtt cgagatcggg
     3481 caccagtacg tgagcgggga cgtcgagttg gaggagatgg tctcgggaac cgcggtggag
     3541 cgctcgcacg gcgtgcccgc gaaagacatc atggatatgg atatctggag ggagtacgcg
     3601 aagcgcttcg cctatgggct ctataacacc ctcctgcact ggtcgccgga tacggtcgtg
     3661 ctcagcggct ccctcatgcg cgagagcggc atgcacgtgg aggacatccg cgctgagctc
     3721 gagcgcatca accgggcgat cccctccctg ccggacatcc ggcatggcaa gctcgaatat
     3781 ccagggctcg tgggcgccct tatcctcacg gactcctaag gtttccctgc tagaatccat
     3841 atccatatgc gctatttcct cgtcccgacc gtcgtctcgg ccctgatcat gctcggcatc
     3901 ctcgtgtggc tcgggcccgt ggctttcgtc accgcggtcc tcctcaccat cctcgaggtg
     3961 acgctctcct tcgataacgc ggtggtgaac gcccgggtgc tcatgcgcat gagcgagaag
     4021 tggcagcagc gcttcctcac ctggggcatc ctgatcgccg tcttcgggac gcggctcgtg
     4081 ctgcctattt tgatcgtgag cgtcggcgtc tgggcctcgc cctgggcggt cgcgcagctc
     4141 gccgccttcg atcccgagac ctacggccac ctcctcgagg aagcaaagca cggtatctac
     4201 gccttcggag cctccttcct catcatggtg tcgctgaagt acttcttcaa cgaggcgaag
     4261 gagacgcact ggatccaggt gatcgagcgg cagctctcga agctcggacg gatcgaggcg
     4321 atcgagatcg gagtggcgct cctcaccatc ctcgccttct ccttcctcgt gcccgacgag
     4381 cagggcgtca tcctcgccgc cgggatcgtg ggcatcgtcc tcttcatcgt catggagggg
     4441 ctcgcgagct cgttctcgga gggggtgaag ggagcgaccg cctcggggct cgcccttttc
     4501 atgtacctca acgtgctcga ctccgcgttc tccttggata gcgtggtggg cgcgttcgcg
     4561 ctcacgacct cgatcccgat catcgtggcc ggtcttggca tcggcgcctg gtacgtccgg
     4621 agcatgacca tctacttcgt ccgcaagaac acgctcgata ccctcgtgta cctcgagcat
     4681 ggcgcgcact gggcgatctt cggccttgcg gtctccatgt tcgcagggct tctctttgag
     4741 gtgccggaga tcgtgacggg cggaatcggg ctcgcgttcg tcgcagctgc gtactactca
     4801 tcgctgcggt tcaggaagat gaatcgggga gtttgacaaa aaccaatata tagtatacaa
     4861 atatagggaa tgtccgggcc tgtgccctgt tgcatcaaca accgaaggag accatccgtg
     4921 aagctttcga tcctggccgt cgctgccgcg ctctcgctct ccggcgcccc tgcgttcgcg
     4981 cagttggctg caatcagttc gagcccgcag ccacgcgcgc gtccggtgac catcaacgcc
     5041 gccgattgct cgcgcctcta cccgctgcag gtgcagaact acgccctgca gtcagacgcg
     5101 gcgcatctgg agttcaagat ggagaagtcg ccgagcgtca cgcagatcat gcgcgacaat
     5161 ctcgccgtca cgaaggcgag cctcgcgagg agcaggcggc acctcgcgca gctcgagaac
     5221 tactgccgga cgtaccaccg tccctggcgc tagcgccacc cgtcgaccgg aacattcaca
     5281 tcagtgcgtg aatgtcccgg ccggcggttt tgttttgtcg cgctatactt tctgtacatg
     5341 agtttgaagg aaacccttgc cgcctccctt accggtgagg tcgacgatga cgcgctcacc
     5401 cgcgataaga tgagccggga cacgagcgta ttccagcgta cgccctccct cgtggtgtat
     5461 ccgaaggata cggaggatgt ctcgaagctc gtgaaggcga tagcgaagga gccgggggtc
     5521 tccgtgaccg cacgctcggc cggcacggat atgtctggcg ggccactcac cgattcggtc
     5581 gtcgccgtgt tcacgaagta catgaacaag gtgcatgagg tcgcggacgg gtacgccatc
     5641 gccgagccgg gcgtctacta ccgcgacttc gagaaggaga cgcgcaaggt gcataaccag
     5701 ctcctcccct ccttccccgc ctctcgcgag ctctgcgcct tgggcggcat cgtgaacaac
     5761 gattcaggcg gcgagcgcac ccttgaatac gggaagaccc gcgactacgt cgaggaactc
     5821 gaggtggtgc tctcggacgg cacgataacg accttcggcg agctcgggcc cgcggagctc
     5881 gcggagaaga aagagctccc ggggctcgag ggcgagatat accggaggat gagcgagctc
     5941 ttgatcgaca accgggaggt catcgccgcg gcgaagccca aggtctcgaa gaactccgcc
     6001 gggtacgcgc tctgggacgt catggatatg gaagacggga ccttcaatct ggctaagctc
     6061 atcacgggcg cccagggcac cttggcgctc acgaccaaga tgaagctccg cttggtgagg
     6121 gagcagggct accgctcgat gctcgtgatc ttcctcaagg acatcgacca tctgccggag
     6181 atcgtgcata aggtgcttcc ctacaacccc gaatccttcg agagctacga cgaccagacc
     6241 ttcgggcttg cggtacgatt cctgccgcag atgctttcgc agatggggtt ggggagagcc
     6301 atccggctcg ggttgtcgtt tcttcccgag gtcggcatgg cgatgcgcgg cggcgttccc
     6361 aaactcatcc tcatggcaga gttctcggag gattcccgcg aggaggcctt gaagaaggca
     6421 cgcgaggccg aggccgccat caagcacctg aagctccaga cctcgatcaa gaagaacgag
     6481 caggcggccg agaaatactg ggtggtgcgg cgggaatcgt tcgcgctcct gcgcaagaac
     6541 tcgcacgggc tctatgcttc ccccttcatc gatgacttcg tggtgccggt cgattcctac
     6601 ccggagttca tgcccgaact gaatgcgctc ctcgccgagt acaaggacaa gttcgtgtat
     6661 acgatcgcgg ggcacatcgg gaacggcaat ttccatatca tcccgctcat ggatttgaat
     6721 aaggaggagt caaggaagat catcctcgag ctctcgcccc gggtgtacga actcgtcatc
     6781 aagtacggcg gtactacgac cggcgagcat aacgacggca tcatccggac cccgtacctt
     6841 cccatgctct tcggggagaa gatggtgtcc ctcttcgaag agaccaagcg catcttcgat
     6901 ccgaacaaca tcttcaatcc gggaaagaag gtgggcggga cgttcgcgga tatcgagaag
     6961 tccatcctga agcatatata gcgcgtgcta cagtacgcat atgagcgatg aacatcgcgc
     7021 gcatgaagag ctctccaagc agctgaacga cccgaagctc cgccacgagt acgcgaagcg
     7081 ctacgaccac taccaggtcg ggtggctcgc gcgcttcctc gggtggctcc tcgtctcctc
     7141 cggggacctc gtgtacggca cgaagccgtc gtacgcaaag ttcaaggcca tcgaagtcgt
     7201 cgcccgggtg ccataccagt cgtgggaggt cgcggcgtat accctcctca gcgccttcta
     7261 taggaacgag aagcgcgcga tcgagctcgc gaagacgagc gccttcagcc ggctcgcgca
     7321 ggacaatgag acgctgcatg tcatcgtgct ctcgcagatc gtctgcaagc tgaagcagca
     7381 acgcttcttc aggcacacgc tcgtcccctt cctcttcgcg ctcttctatt tcatcgccgt
     7441 gtatttcctc tatctcgtgt accgaaaggc ggccctggaa ctgaactatg tcttcgagaa
     7501 ccatgcctac catcagtacg cggagttcct ggagcgcaac gaggcggtcc tgaagcaccg
     7561 catctgcatg tacgagttcc tctccttcta cggccgcaag gtggtgacgg agtacgagtt
     7621 cttcgagagc gtccgcaacg acgaactcat ccaccgcaac accaccatcc gggagctcga
     7681 ggcgcgcggg tacttctgca tgccgaagat gccggaggag gataatccac agggggatgc
     7741 ggcaaacctt gcgcgttcat gaggtttgta gcatagtgga gctaactcgc aggtgaggga
     7801 agcttcgctg ggttcatccc ctccaagtgt tcgatatcgg cgaggttata cgtatttttc
     7861 tagccacatg gcatcaaaca agctctatgt cggcggtatt ccttaccgca cgaccgagga
     7921 cgagctccgc accgctttcg aggaagcggg cacggtgacg tccgtcagca ttatcagcga
     7981 tcgtatgacc ggccgctcgc gcggattcgg cttcgtggag atggcagacg aggcccaggc
     8041 ccaggctgct atcgaccggt gggacggcaa ggaaatggac gggcgcatgc tctccgtttc
     8101 catggcccgc ccgcagggcg accgtcctcc tcgtgagggc ggtggcggcg gtcgcggggg
     8161 cttcggcggt ggaaaccgcg ggggcttcgg cggcggcaac gatcgcggag gctacggcgg
     8221 gggttactaa ctctctctga aagaaaaggc ccggggcttg cccccgggcc ttttcttatc
     8281 cgcaggtgcc gccctgcccg ggaccgctcc ccagtacgct gcgcttcttc gcttccgcgc
     8341 tccagccgac gaggttcgag aggatctgga ggaaggaacc gatcgccgcg agcgcaagga
     8401 tgaggatgac cggccaggcg tagatggtgg cgaaatagaa gaagaccaca atgaaggaga
     8461 accagaggcc gatgcaccac gggcagttga tgagggtctt cagggtcccg aggaaggtat
     8521 ggggtgcggc gttcgcgaac cagtcgcgga cgaaggccgt gatggcgtcg taggtgaaga
     8581 ggcggatgag gcgccaggtg gcgagcgcca tgaggacgaa gtcgcccaag gagaccgggc
     8641 gatcgaactt gcccgtcatc gcgagccagg cgatgccatt gaccaagagg aagaggaaga
     8701 atacggagaa ggcgatattc cagaaggctt tcatggctcg atgatagcac gaggccctgg
     8761 cagagccggt gcctcgtgca gtcgcgctta ggcgcagtat ccgcggggga tgaagctgcc
     8821 cttctgggcg gcggtatacg ggcggtcggt gccgacgggc actttccggc tcgaccacga
     8881 agcaggagtg atgcggtgct cgtagcggac cttgggcttg agcgacctac cggagatcac
     8941 cgcgtcgaca tcgaagcact cctcgagctc ctccttgctc gtgaactggt ggatgcagac
     9001 ggagaggatg gcatggacgt cgtgggagag ccccgcgcat accgcgtcgg gtgcgagctt
     9061 gggcttcttg gctacggatg acgtatcatt ggtcatgggc cgtatataca gtatattcta
     9121 gcatatgtca ataccctccg cagaggatgt caaggcgctc atacgggaca agtacgacgg
     9181 ggttgagtcg tctgcgcttg ctggggatat cgcccggctt gcggccggtg agccgcttgc
     9241 gtacgtcatc gggtgggtgc ccttcctggg gctccggatt gacctttcct cgcggccgct
     9301 catcccccgc cccgagaccg aatactggac ggaggtactc atcggggagc tgaagaagcg
     9361 ccgcgtgcca cgcgtgctcg acctttgcgc cggctccggg gcgatcgggc ttgcggtcgc
     9421 caagcacgtg ccgggcgcga cagtctcctt cggcgagctc gagcttgcgc ataccgcgca
     9481 gatccgcaag aacctggagc tcaacggcgt tggcggcacg gtgcggcgtg ggaacctctt
     9541 cgcgcccttc ttcgacgagc ggttcgatgt catcgcgacc aatccgccgt atgtcccgga
     9601 gggccgaacg cttgaggaga gcgttgctgc acatgaacct ccggtcgcgc tcttcgccgg
     9661 ccccgcgggg ttgtccgtca tcgagcggat cgcacggg
//
LOCUS       DASPKG010000052         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_492050, whole genome shotgun sequence.
ACCESSION   DASPKG010000052 DASPKG010000000
VERSION     DASPKG010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492050"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VEA36_02015"
     CDS             <1..727
                     /locus_tag="VEA36_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HYF29122.1"
                     /translation="NAVTAKNIFKLLQYCGLPVVYLGDCENDNEFYVLRTKMLRWELV
                     VRRRSRGSMPHRHPKFPNGHRFDSPVFELYLKTSGKTWVGRKRYDLVCDDPLVRLNGR
                     SAELYHPKRQFKPGTYFLRMDRAEAFTVEDEERLLKQAEELALSAFLILEYAFKLLGV
                     TLDDFKIEVGIGPDGKLYISDDINCDSWRARKGNYQLSKEPFRNGESAAKTIKRYQAA
                     AEESNNLHFLEPMVRTWWLAGRH"
     gene            827..>2208
                     /locus_tag="VEA36_02020"
     CDS             827..>2208
                     /locus_tag="VEA36_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29123.1"
                     /translation="MEGPVRPIASPEEELAYLRAQVAAKEAELNQLKESRPREEIAHE
                     RIVHHRTEAPSGHFAEGYRMHDSEATALSLNLDPEQNDETMEELRGIMETKGVRNALA
                     VLEKLQSPHLDDDFHRYLVQYLLAGMKVPGMREHEPEWKALHMTLYEVALPEATSSEE
                     EKTRVKPLKELISSMEQFYAGMLSLEHAHKGEPPYFSLELAVPVNAPHLRFYAAVPNT
                     RRDLFEKQLLAIFPNARISVQPNDYNVFVEGGANQGSYALLADKPIAPLRDYADFDYD
                     PLNSILNAFEKIAHEGEGASFQMILKPAGNHYIEYYRKVLHALRQGQPRAKAFALPET
                     FVGDLAKEAFNIVAQSKKNPYDPSKADQAAIEAVEKKIASPVLLANLRLVVSSADPVR
                     AQHLLTELEASFNQFENTKGNRLTWKRAERGEAKKLFRNFSFRLFADHAKLPLSLKEV
                     TTMYHFPPKG"
BASE COUNT          447 a          725 c          661 g          375 t
ORIGIN      
        1 caacgccgtc actgccaaga acatcttcaa gctactgcag tactgcgggc tcccggtggt
       61 ctacctcggg gactgcgaga acgacaatga gttctacgtc ctcaggacca agatgctccg
      121 ctgggaactc gtggtccgtc gtcgttcgcg cggctccatg ccgcatcgcc accccaagtt
      181 cccgaacgga caccgtttcg actcgcccgt cttcgagctc tacctcaaga cgagcgggaa
      241 gacatgggta gggcggaagc gctacgacct cgtctgcgac gatccgctcg tgcggctgaa
      301 cggcagaagc gccgagctct accacccaaa gcgccagttc aagccaggca cctacttctt
      361 gcgcatggac cgtgcagaag cgttcaccgt cgaggacgag gagcggcttc tgaagcaggc
      421 ggaggagctc gccctaagtg cgttcctcat cctcgagtac gcgttcaagc tgctcggtgt
      481 gactctcgac gacttcaaga tcgaggtcgg catcgggccc gacggtaagc tctacatctc
      541 tgacgacatc aactgcgaca gctggcgtgc ccgtaagggc aactaccagc tctcgaagga
      601 gccgttcagg aatggcgagt cggccgcgaa gaccatcaag cgttatcaag ctgcggccga
      661 ggagagcaac aacctccact ttctcgagcc aatggtgcgg acctggtggc tggcaggtcg
      721 ccactagttc tcgttcgagt cgacgggcgc gcatgcgcgt ccgtcgattt gcttttatcc
      781 tcccgtcccg catttcgata ccctacatcc cgtatactga acagtaatgg agggccccgt
      841 acgtcccatc gcctcgccgg aggaggaact cgcgtacctt cgcgcgcagg tcgctgcgaa
      901 ggaggcggag ctcaatcaac tgaaggaatc gcggccgcgc gaggagatcg cgcacgagcg
      961 catcgtgcac caccggaccg aggcgccgtc cgggcatttc gcggagggat accgcatgca
     1021 cgactcggag gcgaccgcgc tgtccctcaa cctcgatcct gagcagaacg acgagaccat
     1081 ggaggagctc cggggcatca tggaaacgaa gggagtgcgg aacgcgcttg cggtcctcga
     1141 gaagctccag agcccccacc tcgatgacga tttccaccgc tacctggtgc agtacctgct
     1201 cgctgggatg aaggtgcccg ggatgcgcga gcatgagccc gaatggaagg cgctccacat
     1261 gaccttgtac gaggtcgcgc tgccggaggc caccagcagc gaggaggaga agacgcgggt
     1321 gaagcccctg aaggagctca tctcctccat ggagcagttc tacgcgggca tgctctccct
     1381 ggagcacgcc cacaaaggtg agccgcccta tttctcgctc gagcttgcgg tgccggtgaa
     1441 tgcgccgcac ctgcgcttct atgccgcggt cccgaacacc cgccgggacc tcttcgagaa
     1501 gcagctcctc gccatcttcc cgaacgcgcg catatcggtg cagccgaacg actacaacgt
     1561 cttcgtcgag ggcggggcga accagggatc ctatgccctg ctcgcggaca agccgatcgc
     1621 gcccctgagg gactatgccg atttcgatta cgatcccctc aattccatac tgaatgcctt
     1681 cgagaagatc gcgcatgagg gggaaggcgc ttccttccag atgatcctga agcccgccgg
     1741 gaaccactac atcgagtact accgcaaggt gctccatgcg ctgcggcaag ggcagccgcg
     1801 cgccaaggcc ttcgcgcttc ccgagacctt cgtcggggac ctcgccaaag aggcgttcaa
     1861 catcgtcgcg cagtcgaaga agaacccgta cgacccctcg aaggcggacc aggccgcgat
     1921 cgaggcggtg gagaagaaga tcgcgtcgcc ggtcctgctt gcgaaccttc ggctcgtcgt
     1981 gtcgagcgcg gaccccgtgc gggcgcagca cctcctcacc gagctcgagg cttccttcaa
     2041 ccagttcgag aacacgaagg gcaaccgcct cacctggaaa cgggcggagc gcggcgaggc
     2101 gaagaagctc ttccggaatt tctccttccg gctcttcgca gaccacgcca agctcccgct
     2161 ctccctgaag gaggtcacga ccatgtacca cttcccgccg aagggcat
//
LOCUS       DASPKG010000053         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_86662, whole genome shotgun sequence.
ACCESSION   DASPKG010000053 DASPKG010000000
VERSION     DASPKG010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86662"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VEA36_02025"
     CDS             <1..881
                     /locus_tag="VEA36_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HYF29124.1"
                     /translation="LKSLAESIRQYGILQPLTVTRKDVEKADGGLSSMYELIAGERRL
                     RASKLIGLKEVPVVIRVGEQSDKMKLELAIIENLQREDLNAIDRAKALHQLVDSFGLS
                     HSEVAAKIGRSREFVSNSIRLLNLPEQIQQAVVNRELTEGHARALLMLADRPQEQETL
                     FKEIILKKTSVRIVEQMARRIATDKIRKHDKTPEVMEMEKSLTEALGTRVVIENRPKG
                     GRVLIEFFSPEDLTHILAALAAQQESKTVMADQPADVTDEQLAETTPMLHPNTSSIAE
                     APKQDHELYSVRNFTV"
     gene            complement(882..>2022)
                     /locus_tag="VEA36_02030"
     CDS             complement(882..>2022)
                     /locus_tag="VEA36_02030"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HYF29125.1"
                     /translation="ADRLGMGRLKKELEDLAFPAVDPDAYHHAVEVRKLKTKETEAGL
                     ARVQKDLQHALVRKGIQNFSTAIRMKGLWSLHQKLKRKGDDITRIHDIAALRVIVPTI
                     DDCYTALGVIHALWKPLPGEFKDYISFPKPNGYQSLHTTVITPEAGIVEMQVRTGEMH
                     QRAQFGIASHMSYKELGKAAPKAAFRRLSFPWVRDLIPTLLNFKRQPQPAAPEKKPAD
                     VPRWLTELADAHASVAGTNDFVEGLKSDFFSHRVFVFTPKGDVIDLPIESTPADFAYA
                     IHSDLGDHMQGAKVNGKLVSFDTKLRNGDIVEIIRRESAHPTAKWLDFVKTSVARKHI
                     RAALEEHERPVVRDPIKSVKKEQRLEQKKQERRNAKKAFKKKKGK"
BASE COUNT          338 a          640 c          667 g          377 t
ORIGIN      
        1 cgctcaaatc gcttgccgaa tccatccgcc agtacggcat cctccagccc ttgaccgtga
       61 cgcgcaagga cgtcgagaag gcagacggcg ggctctcttc catgtacgag ctcattgccg
      121 gggagcggcg gctccgcgcc tcgaagctga tcggcctcaa ggaggtgccg gtggtgatcc
      181 gggtgggcga gcagtccgac aagatgaagc tcgagctcgc catcatcgag aacctgcagc
      241 gcgaggactt gaacgcgatc gatcgcgcca aggcgctcca tcagctcgtg gactccttcg
      301 gcctctcgca ctcggaggtc gcggcgaaga tcggccggag ccgcgaattc gtctccaatt
      361 ccatccgcct cctcaacctc ccggagcaga tccagcaggc ggtcgtgaac cgggagctca
      421 ccgagggtca cgcccgcgcc ctcctgatgc tcgcggaccg tccgcaggag caggaaacgc
      481 tcttcaagga gatcatcctc aagaagacct cggtgcgcat cgtcgagcag atggcgcgcc
      541 gcatcgcgac cgacaagatc cgcaagcacg acaagacgcc cgaggtcatg gagatggaga
      601 agtcgctcac ggaggcgctc ggcacccgcg tcgtgatcga gaaccggccc aagggcggcc
      661 gggtcctcat cgagttcttc tcgccggagg acctcaccca catcctcgcc gccctcgctg
      721 cgcagcagga gagcaagacc gtgatggcgg accagcctgc ggacgtgacc gatgagcagc
      781 ttgcggagac gacgcccatg ctccatccca acacctcttc gatcgcggag gccccgaagc
      841 aggaccacga gctctactcg gtgcgcaact tcacggtcta gctacttccc ctttttcttt
      901 ttgaacgcct tcttggcgtt gcggcgttcc tgcttcttct gctcgagccg ctgctccttc
      961 ttcacggact tgatcggatc gcggaccacg ggccgctcat gctcctcgag cgcggcgcgg
     1021 atgtgcttgc gggccaccga cgtcttcacg aaatccagcc atttggcggt ggggtgcgcc
     1081 gactcccggc ggatgatctc cacgatgtcg ccgttgcgga gcttggtgtc gaaggagacg
     1141 agcttgccgt tcaccttcgc gccctgcatg tggtccccga ggtccgagtg gatggcgtag
     1201 gcgaagtccg cgggcgtcga ttctatcggc aggtcgatca cgtcgccctt gggcgtgaac
     1261 acgaacacgc ggtgcgagaa gaagtcggac ttgaggcctt cgacgaaatc gttcgtgccc
     1321 gcgaccgatg cgtgggcgtc cgcaagctcg gtgagccagc gcggaacgtc cgcgggtttc
     1381 ttctctggtg cagccggttg cggctggcgc ttgaagttga ggagcgtggg gatgaggtcg
     1441 cgcacccacg gaaaggagag ccggcggaag gcggctttgg gcgctgcctt gccaagctcc
     1501 ttgtacgaca tgtgggaggc gatgccgaac tgcgcgcgct ggtgcatctc gcccgtgcgc
     1561 acctgcatct cgacgatgcc cgcctccggc gtgatgacgg tggtatggag cgattggtag
     1621 ccgttgggct tcgggaagga gatgtagtcc ttgaactcgc cggggagcgg tttccagagc
     1681 gcgtggatga cgccaagcgc ggtgtagcag tcgtcgatcg tgggcacgat gacgcgcagc
     1741 gcggcgatat cgtggatgcg ggtgatgtcg tcccccttgc gcttcaattt ctggtgtagg
     1801 ctccagagcc ctttcatgcg aatggccgtc gagaagttct ggatgccctt gcggaccagg
     1861 gcatgctgga ggtccttctg cacgcgcgcg agccccgctt cggtctcctt ggtcttgagt
     1921 ttgcgcacct cgaccgcgtg gtggtaggca tcggggtcga ccgccgggaa ggcgaggtcc
     1981 tcgagctcct tcttgaggcg ccccatcccg agccggtccg cg
//
LOCUS       DASPKG010000054        35497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_627006, whole genome shotgun sequence.
ACCESSION   DASPKG010000054 DASPKG010000000
VERSION     DASPKG010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 35497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35497)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35497
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627006"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VEA36_02035"
     CDS             complement(<1..333)
                     /locus_tag="VEA36_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29126.1"
                     /translation="MFFYTPTRGMSLMDVIIGSAVTLIVFVGLFGLLRASFVVSSLTK
                     SKAIATSIAARHLEYIRSLPYDELGTQGGIPAGVIPQIVVSTENGISFETRTFVRYVD
                     DPADGLGPA"
     gene            701..1537
                     /locus_tag="VEA36_02040"
     CDS             701..1537
                     /locus_tag="VEA36_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HYF29127.1"
                     /translation="MDIRATYHRLIETIEDAGTLAFAYFNANESANELKDDGSVVTKV
                     DQEIETRLLAFINQQFPDDAIVGEEHGSKDGTSGFVWHVDPIDGTDNFIRKIPFCAVS
                     VARLGDTAEDSFGIVHNPFTRQTFSSLMDEGVYENEHVHNLTADPLGGRYVISIGRGK
                     TEEWMRPAGFALQKELAVAFGKCHAYGSSALELAYVAANRIDAYLTFGLKSYDYAAGL
                     FLVASAGGAISVFEEGAWRRWTGSLKELCSTHERIIFASHPDIHAKMLDFIGDPHAWA
                     DR"
     gene            complement(1538..1837)
                     /locus_tag="VEA36_02045"
     CDS             complement(1538..1837)
                     /locus_tag="VEA36_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29128.1"
                     /translation="MTPFRHATAAALYIALLVTSVWSISTFFEPKETILVPMAVLGTL
                     VFSAALMAYLFFAKPLILFRDGKAEEGIRFFLTTLGIFMGYVLLAIVLVFALSYL"
     gene            complement(1948..2616)
                     /locus_tag="VEA36_02050"
     CDS             complement(1948..2616)
                     /locus_tag="VEA36_02050"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYF29129.1"
                     /translation="MSLKQLERELDASPAMASHHPFFSRFSGEKTSEQIARFAAQWYL
                     AARNHKRAFPFLVAITEDDETRRELIEILRDEYGNGEPDKVHARLLRKFMIEGAGLSA
                     DQIDSLEMIPEVAEFGETTLKVWRDGDPIVAYGYHYALERIAASIHPAFFEGLKGNGF
                     SPDSLEYFQYHSRAEEEHVHAAEAGFLRYDAEGDGEKLRQGAELGAAALAKMWDGFDR
                     HVFH"
     gene            2763..3578
                     /locus_tag="VEA36_02055"
     CDS             2763..3578
                     /locus_tag="VEA36_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29130.1"
                     /translation="MKMTFSSLENSILMEVIHTPRSVAELYQKYVERSRCSRAGFYKA
                     VASLKKKEVVVEEGRLLSVNKIWLADSYDFFRKLVRQKTTPSYLAQEVSRLNKGDRLS
                     YTFHSIAEIDIFVLNLLYDLLLLKIDTKALILEAHEFFVLLNDSRTRRIMGELANIGG
                     RVHVLIEGGTALDKEVVRRFLPKPAEGHVVDTKSASSSNILHVIGDVCIELKLNKQLA
                     VSLNHLFAANEKMTDGFRTDLENLLSKKRKHEVVIYRDSSKASRLRGRFKKYF"
     gene            complement(3641..4858)
                     /locus_tag="VEA36_02060"
     CDS             complement(3641..4858)
                     /locus_tag="VEA36_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYF29131.1"
                     /translation="MKYRVTLKRADGSEEKRTMDAPSRFAVYTDAEKEGATVVSVDEG
                     GGMPPFLAKLAEVQIGSGVKMEERITFTKNLSAMLTAGLSLSRALSVIERQSTNKSLK
                     KIVVDLSERVKKGEAFHEALAAHRNIFSDLFIAMTKAGEESGTLAEALKVVARQMDRA
                     HSLNKKIKGAMIYPSIILVAVVLIGILMLIYVVPTLSNTFKELGVELPAATKAVIGAS
                     DFMASHPILVLTLFAIIVAGIILFTRTKTGSSIVVAVALRIPVIGELVRETYSARAAR
                     ALASLLSAGVEMLSALSITAEVVGERRFSKVIAEAETRVRKGETLSATFNDYPKLYPV
                     FIADMIAVGEETGKVADMLGQVAEYYESDVEDRTKDLSTIIEPVLMLFIGGVVGIFAL
                     SMISPIYSLSDKI"
     gene            complement(4931..5302)
                     /locus_tag="VEA36_02065"
     CDS             complement(4931..5302)
                     /locus_tag="VEA36_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29132.1"
                     /translation="MKSLMTVSAIVAAIVVGLASHAMLGDVPYLMKVPHVVPAGVLAA
                     LIALLLLHISRAELVPIAAFTIGLFFVGLIFGTIGASYGPQMLVIGSVCWLVALAGCV
                     PLAFTWFVQIVVGHSLAESAK"
     gene            complement(5427..5639)
                     /locus_tag="VEA36_02070"
     CDS             complement(5427..5639)
                     /locus_tag="VEA36_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29133.1"
                     /translation="MSELRRVPSGKCIVGEVNDTHGGLVATSEPCELMEALALARSFS
                     KSFAHHVYLVLDHQDNIVLNVTARAA"
     gene            complement(5705..7465)
                     /locus_tag="VEA36_02075"
     CDS             complement(5705..7465)
                     /locus_tag="VEA36_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HYF29134.1"
                     /translation="MQVSESELKEFILDSGLVARKDVEDAAKEAEERGQSIGDILVSR
                     GTLSADALRRIKAYVLGIPFVNLKDRKIPLDVLSLIPEPIARTHSIVAFRKSGNDLEI
                     AMLDTEDLASIESVKKKTGLKILPRLTDDESLKSVLVQYQKSLKEEFGDLISTEAGKL
                     NLIKEAGGEEVSGEDLKKMAEDLPIVRIVDTLLRHAIIQDASDIHIEHSETEVIVRYR
                     IDGVLHDAMTLPKNAAAGLVARIKVLANLKLDEKRLPQDGRFKMQTESEAVSFRVSLL
                     PTYYGEKVVMRLLREGGAEGFSLEALGFHGETLERIHTATRLTTGIILVSGPTGSGKT
                     TTLYTILDILNTPDVNISTVEDPIEYQMKRVNQTQVNPAIGFTFANGLRALLRQDPNI
                     IMVGEIRDGETAALAINAALTGHLVLSTIHTNSASGAIPRLIDMGVEPFLMVSTLRVV
                     VGQRLVRRLCEGKEQYTLDSEERAKVAHEDKFEAALKALHDEKLVKTTVKLDTLPFYK
                     PTPSTECEEGYKGRIGIHEVLTVSPAIRDLILHSGTSDQIEEQARKEGMLTMLEDGLY
                     KAAKGTTSIEEVLRAVTDSA"
     gene            complement(7497..8240)
                     /locus_tag="VEA36_02080"
     CDS             complement(7497..8240)
                     /locus_tag="VEA36_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYF29135.1"
                     /translation="MAKSTEITASLEGLGLGANEAAVYIAMLSLGPATVLSISRASGV
                     KRTTIYNVLESLKQKGLVRTDVRGFKQLYAAEHPKKLESMLEQRRGALLDLLPELEAQ
                     FDLKRGESFIKYYEGVGAIRNAYFEMLEGLNHDDEFLVVGDPDRWEDMNGSFATEFIR
                     ERNRKKLRIRMMLVDSSTARRYKEHETNFQETIKMLPADSKVDTNLVVTPRKVIIQQM
                     LKPSILVSIENKSIITMHTELFNVMWRNL"
     gene            8411..8542
                     /locus_tag="VEA36_02085"
     CDS             8411..8542
                     /locus_tag="VEA36_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29136.1"
                     /translation="MKTSYIAMAGQGVSSHAVISAAQGKSSSPVMAGQGASSFIAKQ"
     gene            8605..10068
                     /locus_tag="VEA36_02090"
     CDS             8605..10068
                     /locus_tag="VEA36_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HYF29137.1"
                     /translation="MQQPTITFFPGKGIYSLPAGEEIVSVHATEEARAIFAGDASVRG
                     KAKQLQRFYEQQNIAIEQKPVLSETRRFDFGTVEHFGTRSSAHVCAVDWGAVEDALAQ
                     MGYHLRQRTPISDEIGRLAPVYLIEGPEGVLAEGKGLDQEQAKRSTLGEAVERLAARG
                     PSERVRAQRGTDTYQNLYRGKDWGLGTLAGPRDAFDSQIPSEWLPARNISRDETAFLP
                     AELSYFEYVPETTRTRLFSLHHTMGLAAGSSREDAALSGIFEVIERDAYWITMRCRMN
                     LPDIPLEAVRDLDPKVIAIVQALEKRGFKLSVKDLSLDWGVPVVHAVLVDTQGGIPAF
                     AHGTGASFSWATAVARAVCESVQMYEGLSAVMNDEWKWEDVVSVAGVLGNAAYAWSDP
                     LFRPHIEHLLAPSDQPWDGSEPAWSVPEFLADLKQRGHEVIVADLGSDYGLEVVRVFI
                     TNTTQPDPRLERISLRLEAWRNRLGLERGLYTDPILT"
     gene            complement(10065..10556)
                     /locus_tag="VEA36_02095"
     CDS             complement(10065..10556)
                     /locus_tag="VEA36_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29138.1"
                     /translation="MEHDLSRRATPEELEDILRSPVYAPWTLKEYEHWSLRLNITKQE
                     LPGRSTAWLKRHVDQMSLAELTKEELHELVYTIIPEFTDAMRSWLPLRTLNVEWLGNE
                     TKHHRGHGHMHFTPRLDAPLTFNGREYVDLTPTTRKSNPRLTLSEEELRPIFEHLKGL
                     LNN"
     gene            10685..12121
                     /gene="nusA"
                     /locus_tag="VEA36_02100"
     CDS             10685..12121
                     /gene="nusA"
                     /locus_tag="VEA36_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HYF29139.1"
                     /translation="MLDLKTINSVLGEFEERGITKATMIDAIESAMATAYKKEYGKRG
                     QVIRGKLDMDTGTVSFEQVKTVVDDTMVRFLEEGEETNELPAHHPHHDREEEVAEGEL
                     PRFDAEKHMLIEDAKRIKRDVTLGEELIFPLEPQDDFGRIAAQTAKQVVIQKIREAEK
                     LSILDEFAHKKGEIVSGIVQRIERGAIFVDLGRATGIIPYEEQIPGERYRTGERIRAY
                     LYAVDEGFRGVYLRLSRSHPRFLIRLFEMEAPELASGSIEVKGVAREPGSRTKIAIAS
                     VDPHVDPVGSLVGQRGVRVSTVMSELGGERIDIIEWSEDQAAFIKESLSPASVLTVDL
                     DETNHRAKVTVSEDQQSLAIGRGGQNVRLAAKLTGWNIDIESTGGPVGGAGADEISFD
                     KQKAHDSTIAAEAEIEAEEAGVEDSPSIEAGIAPNENESVITSEEDASDDTGALTVED
                     ETKLGDDGTPVNPDDRDVTSESQSANQA"
     gene            12130..12654
                     /locus_tag="VEA36_02105"
     CDS             12130..12654
                     /locus_tag="VEA36_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HYF29140.1"
                     /translation="MNLLHDIPAGSKDEMNVIIEVSKGSQNKYEIDKETGLIKLDRAN
                     YSAAAFPAEYGFVPQTLWDDGDALDVVVLTTFPIPTGVLVKVRPVALMEMIDTGENDY
                     KVIAVPVKDKRWEDVRDLPDLNKHTLKEIQHFFETYKQLKGEEAVVTINGFKGKADAE
                     AAFEHARKLYEEKK"
     gene            12743..13054
                     /locus_tag="VEA36_02110"
     CDS             12743..13054
                     /locus_tag="VEA36_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29141.1"
                     /translation="MEPSGGPIPLAPKEPVLPQPIVPVAEPRPVIPPPPPPRPLPPPI
                     PPPPIPPPVVKPAPATPKAKGGWGAIIAIFLIVALIITAAFYSWGERLATEQSLEEIA
                     K"
     gene            13100..13699
                     /gene="clpP"
                     /locus_tag="VEA36_02115"
     CDS             13100..13699
                     /gene="clpP"
                     /locus_tag="VEA36_02115"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HYF29142.1"
                     /translation="MNNAPRASMLVPMVVEKSPFGERAYDIYSRLLKERIVFLGGGID
                     DDIANLIIAQLLFLESEDPKKDISLYINSPGGSVTAGLAILDTMNHVKPNVSTVCVGM
                     AASAAAVILSAGEKGKRYALPNAEVMIHQPWGGAQGQATDIEITAKHIIATRERLNKI
                     LAKNTGQPVEKIEKDVDRDYFMMADEAKKYGIIDSVYKK"
     gene            13877..15406
                     /gene="rny"
                     /locus_tag="VEA36_02120"
     CDS             13877..15406
                     /gene="rny"
                     /locus_tag="VEA36_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HYF29143.1"
                     /translation="MSIKLILALLALSGLAGIALGYVLRLLIALGQRGSLELEVKQKL
                     LEAKERANRIITEAEARAEKLEAEIALPLKTREDAIAKSAERLAKREEFLDERERTLA
                     SEAEDVRNRENEAANLKREAEKLLKERRLELVRMGRLSEAEARTLLMKDLEETETETL
                     LARLQKLEAHGRKQLEEKARAILLATIHRLGSSVNADIMTLSVTLPSEEAKGKIIGKE
                     GRNIKTFERATGVDVIIDDAPDKISLSSFDPLRRHIAKLALENLLEDGRIQPAKIEET
                     VEKAKKQVQDMVKQKGEEAAYEVGVVGLDPRLVALLGRLHFRTSYGQNVLQHSIEMAH
                     IAGMLATELGADVDVARAGALLHDIGKAVDHEVQGTHVEIGRRILEKFGVAKEVIQAM
                     QSHHEEYPYETPESVIVQVADAVSAGRPGARRDTVERYLERLGDLERIAKGFEGVEKV
                     YAISAGREIRIFVNPGKVSDLEMHRLARQIAERVQAELKYPGEIKVNVIRENRVVEFA
                     R"
     gene            15583..15855
                     /locus_tag="VEA36_02125"
     CDS             15583..15855
                     /locus_tag="VEA36_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HYF29144.1"
                     /translation="MNKADIAAKVQETLGSTKADAERAVETIIEAISSGLKSGDEVSI
                     AGLGIFSAKARPARTGRNPRTGETIKIAATRTPKFRAAKALKDAVK"
     gene            complement(15903..16661)
                     /locus_tag="VEA36_02130"
     CDS             complement(15903..16661)
                     /locus_tag="VEA36_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYF29145.1"
                     /translation="MDEQLLISLGLNTREAKVYKAVLRARELDPARLAKATGIKRTTA
                     YSIARSLIEKGLLIEDSSRRPRVFRPAATEDLALAVETERKRSEERQQLLKKLAEEVS
                     MVNAETTYPVPRIKFTEESKLEAYFPQVFPIWMESMIETKEYGFWGYQDATLVEAYED
                     QLHYWWSIAPPELYVKLLTNLSSAERRMMGKYERRDMKYWGEATNFISSTWICGDYVI
                     MINTRQHPHYMVEIHSRLLAHDQREVFRNLWDLV"
     gene            16793..17473
                     /locus_tag="VEA36_02135"
     CDS             16793..17473
                     /locus_tag="VEA36_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29146.1"
                     /translation="MRITLALLLSAMLLGSAQNALADCTDTLYGKAASGYILKDVGAE
                     ASDQPVVQGGATRTCGRLSYDLWVSGGLAGDTANEIDLSVYYDDQVGPLKVQLAAQYF
                     FVSLDDSLGEPKDDIVAGYADLSLPVTRGRLTIAPLVRFVQLVGVKDLPNEGIVQPGV
                     RGSFALDDRTTLSADIRHPISSRGYSFFRVTTSLSRQVTERFAAQAGWEDTDQTPSVF
                     WFGGKYNF"
     gene            17703..18416
                     /locus_tag="VEA36_02140"
     CDS             17703..18416
                     /locus_tag="VEA36_02140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYF29147.1"
                     /translation="METGHRFAGKLSEDYDLVRVMIPHYDEHQDTIHTAIEAFALTHT
                     PIEILPTIDIGCGTGFTAERILDADPRTTLIAIDNEPAMMDQARVRLERFGNRVTFYE
                     MDALEAIGRLPARSAEIVASGCVLHNLPHDYRWRLMRKIGRVLVPGGLFVNADKFGLD
                     DPMEHMLQLAEQLKNISVLDDMGRSDLREAWHAHYLEDEEINFTESEQKALLRESGFW
                     GITTIYRKRLEATIAAFKK"
     gene            18505..19206
                     /locus_tag="VEA36_02145"
     CDS             18505..19206
                     /locus_tag="VEA36_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HYF29148.1"
                     /translation="MHSIYNKSAEDMSEVRSNSVNLIVASTPYNDGTVYGQFVDKTSL
                     EQYCQMMERVIAECARVLHPEGRLIIEVADTAIVQGHFVQLAALWQQAALRSQLHLSE
                     RHINFVQTHKGNEDATHGDGFISPPVTAELDETHSNSCQWLVFTKKSSEFLGGNIYYQ
                     NYVYDLEEHHCPYTDEFCRTFFEIGHFKSGDTVLEVFMGTATLGLHVLEREGIYIGYE
                     IDSIIFARAQQRLAR"
     gene            complement(19234..20391)
                     /locus_tag="VEA36_02150"
     CDS             complement(19234..20391)
                     /locus_tag="VEA36_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HYF29149.1"
                     /translation="MEPLANRIRPQSLDEFVGQEHLVGKNKPLRVAIEKQHRFSFILW
                     GPPGTGKTTIARIYARALAAELYELSAVSAGKEDIRKIVGEGKGPTLGLRPKVLFLDE
                     IHRFNKAQQDFLLPYVESGELTLIGATTENPSFEVIPALLSRARVFVLEPLSEESLRA
                     IVARAKIKLSNDAFDWLLGYANGDARRALTILEGAKSLYGKVSVDTLNKALEDPHLRY
                     DKKGDEHYDTISAFIKSMRASQPDAAMYYLARMVEAGEDPLFIARRMVIFASEDIGLA
                     QPTALVVANAVFRACEVIGYPECAINLAHGVAYLSNAAKDKSAYEALGEAQGDVRRHG
                     NLPIPKKIRNAPTKLMKDLGYKKGYKRYDTESYMPEKLKDRKYFRKEDRAE"
     gene            complement(20422..20745)
                     /locus_tag="VEA36_02155"
     CDS             complement(20422..20745)
                     /locus_tag="VEA36_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29150.1"
                     /translation="MEGNLDSTRKNEVHRSRSGESFAPEVDLNNPRLQIALAIRAFPD
                     APSPSAAFKLWMDKEEDGATLAQRFRGLRDTKGDDFPVNMANPEELDALIAETQQHPP
                     HPTLH"
     gene            complement(20826..21749)
                     /locus_tag="VEA36_02160"
     CDS             complement(20826..21749)
                     /locus_tag="VEA36_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HYF29151.1"
                     /translation="MLNAISKIGLVVLAAVIGAVAVLGFAPTASAQFAQTTQCVFTRD
                     LFLGAFGEDVRCLQRYLNASGYPVAYTGAGSYGREGYSFGPLTQAAVARWQAATGQQV
                     TGVFRVSALAGNPSNPFSNTPIIDTRNSNRNRSNDADRRDAREAIDDAREAIDDARDD
                     ISDARRANKNVRDAEDLLDEARDDLKRARRAYNDGDYDDAVDFADDAEENAEDAVDEI
                     GSSSTSASEANARRAIEEADDAIDDAWEEIDDADDDGEDVDDAEDLLDEAEDEYEDAL
                     DAFDRKDYRDAIDHAEEAEELADDAVDEIDD"
     gene            complement(21836..21909)
                     /locus_tag="VEA36_02165"
     tRNA            complement(21836..21909)
                     /locus_tag="VEA36_02165"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21875..21877),aa:Gly,seq:tcc)
     gene            22053..23015
                     /locus_tag="VEA36_02170"
     CDS             22053..23015
                     /locus_tag="VEA36_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HYF29152.1"
                     /translation="MSSTTPEVTGIFGPAATSSAPLPPKRSPSTLGPLLPQAPAIGLP
                     GTLATLRGAIVNIVCTSKDSRVRSMSGSGVIFDPKGLILTNSHIGQYYLVRDALPKGT
                     ITCTIRTGNPAQTAYRAEPVYVSSQWVAANPRTLVTAGASGTGEHDFAVLAITESATP
                     LPLPGSFPYIALGKGVPVEGAEVALGTYGAQSLSSRQIQNSLFPTLVTAKVRTRFSFD
                     NGQIDLIALTGSAASQQGSSGGAVANASGELIGIITTSTQTGEFSSRETRAVTTAHIR
                     SSFKQDSGEDFDRYFKNGYKADFVDVFTDDSERLGTFLVKQLDR"
     gene            23038..23108
                     /locus_tag="VEA36_02175"
     tRNA            23038..23108
                     /locus_tag="VEA36_02175"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23070..23072,aa:Pro,seq:tgg)
     gene            complement(23275..23348)
                     /locus_tag="VEA36_02180"
     tRNA            complement(23275..23348)
                     /locus_tag="VEA36_02180"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23314..23316),aa:Gly,seq:gcc)
     gene            complement(23382..24506)
                     /gene="lysA"
                     /locus_tag="VEA36_02185"
     CDS             complement(23382..24506)
                     /gene="lysA"
                     /locus_tag="VEA36_02185"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HYF29153.1"
                     /translation="MKQSAFLALTKKYGTPLYVYDRSAIEAQAKKLTSHFPGVAVRYA
                     MKANSNLEVLKIIRKAGIGIEAVSRGEIERAYAAGFAKDGISFTCSNLTESELRYAAR
                     YAGRVYLDSLTQLELWGKGRLGKEISLRLNQGIGGGHHAHVITGGPDSKFGIDKKDIP
                     AAKRIAKKYGLLITGLMQHIGSNVLDSKLYVKAARALITAAADFPDVIAIDFGGGLGV
                     PYKPEEDQLDLAALGKALEKEMEGFAKKQGRGIQFAMEPGRFLVAEAGTLLVTVTDIK
                     ITYRHTFVGLDSGFNHLVRPAMYGSYHPIENLSRKGGKQVPVTVAGNICESGDLFAVK
                     RMMPLPKIGDVLAIRLAGAYGYSMASTYNLRPLPKEIIAG"
     gene            complement(24506..24580)
                     /locus_tag="VEA36_02190"
     tRNA            complement(24506..24580)
                     /locus_tag="VEA36_02190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24545..24547),aa:Arg,seq:tcg)
     gene            24641..26104
                     /locus_tag="VEA36_02195"
     CDS             24641..26104
                     /locus_tag="VEA36_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYF29154.1"
                     /translation="MRFPIPLEVQAVTDGLRKEGFEAYLVGGCVRDLLLNRTPKDWDV
                     TTNARPEDIQRVFPDSFYENDFGTVGVKTGAEDPKLAVVEVTPYRTETGYSDKRRPDE
                     VIFGESLEDDLARRDFTINAIALEDSKGQLVDPYKGQKDIEAKVLRTVGNPADRFEED
                     ALRLMRAVRLMAELGFALDSETAEAIRVKSGNLRHISRERIREEFVRIVESKEPMMAL
                     VLAHQLGILEYIAPDLIRGIGVDQNQAHSYDVFEHNMRALQHAADKDWSLDIRLAALF
                     HDVSKPETRRWSDEKKDWTFHGHEVVGGRTVRKALEDLRFSRETIEKVSKLVRWHMFF
                     SDPDQISLSAVRRMIVNVGPENIEDLLNLRICDRIGTGRPKEQPFRFRRYKAMVEEAL
                     RDPISVGMLKTDGNRLMEQFHVKPGPRIGHILHALLEEVLEDPSRNTEEYLDARTKEL
                     LSLGDQELKELGESGKKRREVEEEREIQEILKKHHVG"
     gene            complement(26532..27326)
                     /locus_tag="VEA36_02200"
     CDS             complement(26532..27326)
                     /locus_tag="VEA36_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HYF29155.1"
                     /translation="MRIMSWNVNGIRAVHRKDLFVPFIKKHKPDILCLQETKAEQEQS
                     PIDLPEYEEYWNSAKNKKGYSGTAIFTKMTPNDVVYGMTEDIAKKYGLADDGYGDPNE
                     EGRIVAADMFDFWVVSVYTPNAKDDLSRLHLRHKQWDPAFLEYMKRLEEEKPVIFCGD
                     LNVAHTPDDLARPDANVGMKGFTDEEREGIDQILKAGFVDTLRLFKQGAGHYTWWSPW
                     GNARERNVGWRIDYIFASKTLARHVKQAGIHPEVLGSDHCPVSIEL"
     gene            27357..27839
                     /locus_tag="VEA36_02205"
     CDS             27357..27839
                     /locus_tag="VEA36_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYF29156.1"
                     /translation="MEDIRHEVVITAIIVKDGKYLITRRSPNKKRFPGMWTVPGGKLE
                     PKDYLSMPKDTKDYWYNVLERVLRREVEEEVGLSITNIEYVTSLATVHADGAPSLVIS
                     CMADYESGEVRLQEEETDQYEWVTLEEARGYELIDGIYDELAMAQMRRRGERIEWQRA
                     "
     gene            complement(27843..28745)
                     /gene="xerA"
                     /locus_tag="VEA36_02210"
     CDS             complement(27843..28745)
                     /gene="xerA"
                     /locus_tag="VEA36_02210"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HYF29157.1"
                     /translation="MDARSMLRQFLEYTEIERGRAVKTIENYEHYLDRFFTQMEIKEV
                     SDITEERIREFRLWLNRQPGTSGTMKRRTQNYYLIALRAFLKFLRKRGIEGLNPERIE
                     LAKLPERQIDLISSAELDRLIKAADGTEEKALRDRAILELLFSTGLRVSELCALNSDI
                     DLSRDDLSVRGKGDKVRVVFLSDASKEAVRTYMKARKDMEEALFVNIPRVGKDSKKTP
                     TRLTPRGVQLIIKHYATKAGITKKVTPHVLRHSFATDLLSNGADLRSVQALLGHASIN
                     TTQIYTHVTDAHLREVHKRFHSRK"
     gene            28802..29548
                     /locus_tag="VEA36_02215"
     CDS             28802..29548
                     /locus_tag="VEA36_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HYF29158.1"
                     /translation="MELLVILPIAIFLAVVFIRQVNQYERGVRFTMGRFSGIMNPGWR
                     IIIPVFQGYQKVDMRTKAVDVPDQNAITRDNVPVRVNAVIYYKVSDAEKAIIEVEDFR
                     YAISQYAQTTMRNIVGEVTLDELLSGRDKIADRIREIVDLETDAWGLKVQNVELKDVS
                     LPEAMERTIAKQAEAEREKRAVIINSEGEVAAAENVAKAAQMLSQIPGALHLRTLQSI
                     NDISSDQSNTVVFTVPLEILKALEGFKRGS"
     gene            29612..29878
                     /gene="rpsO"
                     /locus_tag="VEA36_02220"
     CDS             29612..29878
                     /gene="rpsO"
                     /locus_tag="VEA36_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HYF29159.1"
                     /translation="MLTKRVKTGVIKDAANHPTDTGSAQVQIAILSRKIDELTAHLKK
                     NKKDFHSRRGLLQMVADRRSHLRYLEKSNKRGYNALVKKLGLKK"
     gene            29984..30502
                     /locus_tag="VEA36_02225"
     CDS             29984..30502
                     /locus_tag="VEA36_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYF29160.1"
                     /translation="MPVIKHPAQRVGVFIDTQNLYHSAKHLYKSRVNFANVLEEAVGN
                     RVLVRAIAYVISTESGEEKNFFEALTKIGIETKVKGLQVFSDGAKKADWDVGLAIDAV
                     KLAPKLDTIILATGDGDFVPLIEYLDMNEGCQVEVISFGKSSSAKLKEAAHAFTDMCE
                     NPKRFVIAHRGS"
     gene            30576..31721
                     /locus_tag="VEA36_02230"
     CDS             30576..31721
                     /locus_tag="VEA36_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29161.1"
                     /translation="MSDSPQQPAEDKSRFIRTYAKDVAALTGTSGKAASAPPPPPVPK
                     PVAAPPPPPPKPIPPPPPLEKPEFEAPAPPPAPLPPVNRAPEQPSPIHTYKSDFADRI
                     DQKQASTFSVLAAQKDAGKRPASPKKRRSQLMLAGAVVLIALGGAGLGGAAWYVMRAN
                     MLPAAVPSASSLVFADEQVRLSASGPDLMNEIAALANEPLPDGSVLVVYLSESTTTER
                     GRVIETPLSGGALINAMELPAPQILLRNISPESTVGIMSAGGETRPFFVFKTSSYERT
                     FAGMLAWETSIARDLAVLFPAKAAGSLAPTAASAPLGTSTPVAQPPARPLDAFTDAVV
                     ANHDVRVLRDTAGATLMLYGYAQKDLLILARDEAAFTNLISRLSSGD"
     gene            31746..32099
                     /locus_tag="VEA36_02235"
     CDS             31746..32099
                     /locus_tag="VEA36_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HYF29162.1"
                     /translation="MNIEHFKERLSTEKATLEAELSTVGRRNPANPNDWEAIPQEVGQ
                     EADPNDQADLLEGFRENTAILTDLENRYNDVVAALERIEAGTYGACAACGNPIEEDRL
                     EAEPAAKTCKAHLNG"
     gene            32106..32786
                     /locus_tag="VEA36_02240"
     CDS             32106..32786
                     /locus_tag="VEA36_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HYF29163.1"
                     /translation="MKLSVKECLSFGWETFKKRPWLLAGAVAVGVLVDGLTSLIERAT
                     HGSYILEPLGVLLAILISMLYATGQTNLFVKAHDDPMSARVADLLHLRAYWRFLCTSI
                     LLWLFIGLPYLALEFLSVPPVVMALLIIPALIVSLVFGFALYLVVDRGMGPLQSLKES
                     AQITRGHRLELFLLLLAIVGVNLLGLAALVIGLFVSVPVTVLAMVHAYRMLSGTLPVA
                     VAPAAGAV"
     gene            32801..33487
                     /locus_tag="VEA36_02245"
     CDS             32801..33487
                     /locus_tag="VEA36_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29164.1"
                     /translation="MAALTPYPSLTDYQRFADAVYGLNNRRHFTADEMLTNILRFTMR
                     GLKGIRKGDPEKVRMNMTIAISWTMSLLTQLGVDLERHVWERFPYRCSYCGQCPCACK
                     AKKVQRRVKVVVDPRRRPRTIRDLQVMFNEIYPASSRTLEHAGIHLAEEIGEFSEAVM
                     TYRGNHKDAEFDKVILEAADLYSCFMGVFNSLGIDYEKGLVASFKHGCHACKHIPCEC
                     EYQYVVNFKS"
     gene            complement(33516..34541)
                     /locus_tag="VEA36_02250"
     CDS             complement(33516..34541)
                     /locus_tag="VEA36_02250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYF29165.1"
                     /translation="MIAAGLILSLLALLLPRFAGAFWPFAGARAQVSDTLTHDSSMEL
                     LDAAVNSDPNPAKGGGDLALSGDAALLAKMGPEGAISPRQAAATASEGGGQITTYTVK
                     EGDNVSEIAERFGVTVNTILWANDIKSANLVKPGDELLILPVSGLQYTVRSGDTIAAI
                     AKKYGADPEEVALFNGLESSADLKAGSDIIVPGGSLPTKASAKSTKTAGTKSSGAAAA
                     SRNLPAVSGYFGNPVPGGRLSQGVHGYNGVDIAAPSGTPIYAAAAGTVVVARNNDGYN
                     GGYGNYIVISHGNGTQTLYAHMSRTAVSGGAIAKGDLIGYVGNTGRSTGNHLHFEVRG
                     AKNPFAR"
     gene            34841..>35497
                     /locus_tag="VEA36_02255"
     CDS             34841..>35497
                     /locus_tag="VEA36_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HYF29166.1"
                     /translation="MQHLEGLNDAQKEAVLATEGPISVLAGAGSGKTRVLTTRIYHLI
                     RQGVAPEKILAVTFTNKAAREMRERLAERLGDSSLPIVVTFHGLGRIILERFGSRIGV
                     PRYFSIFDRDDSERAVKEALKALDVDPKEVAPRAILGRISKAKGDGITAQQFKERYGR
                     ESFRNRIAAEVWEHYDATLKKEKALDFDDLICLPVQLLEGHEDLRGEAADLFSHIHVD
                     E"
BASE COUNT         7162 a        10604 c        10746 g         6985 t
ORIGIN      
        1 ggcgggcccg agcccgtcgg ccggatcgtc cacgtagcgc acgaaggtgc gggtctcgaa
       61 cgagatcccg ttctcggtcg agacgacgat ctgcgggatg acgcccgcag ggatgccgcc
      121 ctgggtgccg agctcgtcgt atgggagcga gcggatgtac tcgaggtgcc gcgcggcgat
      181 cgaggtcgcg atcgccttcg atttcgtgag cgacgagact acgaaggagg cgcgcaggag
      241 cccgaagagc cccacgaaaa cgatgagcgt caccgccgag ccgatgatga cgtccatgag
      301 cgacatgccg cgggtgggcg tgtagaagaa catggaacca tgatagcgca cgcgcgccct
      361 tagaagcgga attcgtcgac gaccgtacga tcgcgcgcct catcgcgagc aaggagggtg
      421 agcagcgttg cgtggtgccg gcttccgggc gggaagtccc ctccgtggag atgagctcaa
      481 ccaagcgccc cgcgacggcc gccctaaggt cgcggcggag ctcgtcgagg ccctcgtgct
      541 ccggaaggcg aaacatggca tcacgctgca gtggagcggg gtggcctgcc ctgcccgggt
      601 acggcgtccc taccggcatc ggctcgatgg gaatgagcag gagatgggcg gtggcctcca
      661 tgggcggagt atgcatggcc cagcatgcga tactgtcggc atggatatac gcgccacata
      721 tcaccggctc attgagacca tcgaggatgc aggaacgctc gccttcgcgt atttcaacgc
      781 caatgagagc gcgaacgagc tgaaggacga tggctcggtg gtcaccaagg tggaccagga
      841 gatcgagacg cggctgcttg cgttcatcaa ccagcagttc ccggacgacg cgatcgtcgg
      901 ggaggagcac ggctcgaagg acggcacctc cggcttcgta tggcatgtgg accccatcga
      961 cgggaccgat aatttcatcc ggaagatccc cttctgcgcg gtatcggtcg cgcggctggg
     1021 cgataccgcc gaagactcct tcggcatcgt gcacaatccc ttcacgcgcc agaccttctc
     1081 gtcgctcatg gacgagggcg tctacgagaa cgagcacgtc cataacctga ccgccgatcc
     1141 gcttggtggc cggtacgtca tctcgatcgg ccgggggaag accgaggaat ggatgcgtcc
     1201 cgcaggcttt gcgctccaga aggagctggc ggtcgccttc gggaaatgcc acgcctacgg
     1261 ctcaagcgcg ctcgagctcg cgtacgtggc cgcaaaccgc atcgacgcat acctcacctt
     1321 cggccttaag tcctacgact atgctgcggg attatttctc gtggcttccg caggcggcgc
     1381 gatcagcgtc ttcgaggagg gcgcctggag gcgctggacg gggagcttga aggagctctg
     1441 ctcgacccac gagcggatca tcttcgcctc gcatcccgat atccatgcga agatgctcga
     1501 cttcatcggc gatccacacg cgtgggcaga ccgctagcta gagatacgaa agcgcaaaga
     1561 caagcacgat cgcgagcagg acgtagccca tgaagatgcc gagcgtcgtg aggaagaagc
     1621 ggatgccttc ttcggccttc ccatcgcgaa atagaatgag tggcttggcg aagaagaggt
     1681 aggccatgag cgcggcagag aacacgaggg tgccgagcac ggccatgggg acgaggatgg
     1741 tctccttggg ctcgaagaat gtggagatgc tccagacgct ggtaaccaga agcgcgatgt
     1801 agagcgcggc ggccgtagcg tggcggaagg gggtcatgcc tgtaagaaca aaggtaagaa
     1861 tgagaaggtt acgatagtct gggacgacgt tggaaccctc gtatagagaa gagcccgccg
     1921 aagcgggctc gccgagtaag tccactgtca gtggaagacg tgccggtcga agccgtccca
     1981 cattttcgcg agtgcggccg ctcccagctc tgcgccctgc cgcagcttct cgccgtcccc
     2041 ttctgcgtcg tagcggagga agccggcttc ggctgcatgt acatgctcct cttctgcccg
     2101 gctgtgatac tggaagtact cgagggaatc cggtgaaaag ccgtttccct tgagtccttc
     2161 gaagaaggcc ggatggatcg atgcggcgat ccgttcgagc gcgtagtggt aaccataggc
     2221 gacgatgggg tcgccatcgc gccaaacctt gagcgtggtc tcgccgaact cggcgacttc
     2281 cgggatcatt tcgagcgagt cgatctgatc ggccgaaagc ccggcgccct cgatcatgaa
     2341 cttgcggagg agccgggcgt gcaccttgtc tggttcgcca ttgccgtact cgtcgcgcag
     2401 gatctcgatg agctcccgac gggtctcatc gtcctcggtg atggcgacga ggaacggaaa
     2461 ggctcgcttg tggttgcggg ccgccagata ccactgggcg gcgaagcgtg cgatctgctc
     2521 ggaagtcttt tcccccgaga acctggaaaa gaacggatgg tgcgaggcca ttgccggact
     2581 cgcatccagt tcccgctcaa gctgtttgag tgacattgct gcctcctgtt ggtgggcggg
     2641 tcccggagtt agaacccgaa ggtattttgg aagtgatcgg ccaaatggac aggaaatgga
     2701 tctagatggg ttccttgtct accaaaagta gacaaagtga ggaattcttg tactctcgct
     2761 atatgaagat gaccttctct tcgctggaga attcgattct catggaggta atacatactc
     2821 cgcgaagtgt cgctgagttg taccagaagt atgtcgagag aagccgctgt tcccgagcgg
     2881 ggttctataa ggcagtcgcc tctcttaaaa agaaggaggt tgtagtagag gagggccgcc
     2941 ttttgtcagt caataagatt tggcttgctg actcctatga cttcttccgg aaactcgtac
     3001 ggcaaaagac aacaccgagc tacctggctc aggaagtgtc ccggcttaat aaaggagatc
     3061 gcctctccta tacattccat tcaattgccg agatcgatat attcgtcctc aatcttctct
     3121 atgatcttct ccttctgaag atcgatacga aggcattaat tcttgaagcc catgaattct
     3181 ttgtgctttt gaacgattcg cgcactaggc gaatcatggg agagctcgcc aatatagggg
     3241 gtagggttca tgtgctgata gagggcggga ccgcgcttga taaggaagtg gttaggaggt
     3301 ttcttcctaa acctgccgag gggcatgtgg tggacacgaa aagtgcgagc tcatcaaata
     3361 tcttgcatgt tataggcgat gtctgtattg agcttaagct aaacaagcaa ctagccgtat
     3421 ccctaaatca cctttttgca gcaaacgaaa aaatgactga tgggttcagg actgacctcg
     3481 aaaatctcct ctcgaaaaag cggaaacatg aggtagttat ttaccgagat agcagtaaag
     3541 catcccggct acggggtcga tttaagaagt atttctagac tggagactct tcggataacc
     3601 gaacctgcct cgacttgtct gaaagcccag atttcaaacc ctaaatctta tctgacagcg
     3661 agtagatcgg gctgatcatc gagagcgcga agatgcccac tacgccgccg atgaagagca
     3721 tgagcactgg ctcgatgata gtcgagaggt ccttcgtgcg gtcttcgaca tcgctctcgt
     3781 agtattcggc cacctgcccg agcatgtccg ccaccttgcc ggtctcttcc ccgaccgcga
     3841 tcatgtcggc gatgaagacc gggtagagct tcgggtaatc gttgaaggtc gccgacagcg
     3901 tctcgccctt gcggaccctc gtttccgcct ccgcgatgac cttgctgaag cgccgctccc
     3961 ccactacttc cgcggtgatg gagagcgcgg agagcatctc gacgcccgcc gacagaagcg
     4021 aggcaagcgc acgggccgcg cgcgcgctgt acgtctcacg cacaagctcc ccgatgaccg
     4081 ggatgcggag cgcgaccgcc accacgatgc tcgagccggt cttggtccgc gtgaagagga
     4141 tgatgcctgc gacaatgatc gcaaagaggg tcaggacgag gatgggatgg ctcgccataa
     4201 agtcggaggc gccgatcacg gccttcgttg ccgccggcaa ttccaccccg agctccttga
     4261 aggtattcga gagggtcggg acgacgtaga tgagcatcag gatgccgatc aagaccaccg
     4321 cgacgaggat gatcgagggg tagatcatcg cccccttgat cttcttattg agggaatgcg
     4381 cccggtccat ctgccgcgcc acgaccttca gggcctccgc gagcgtgccc gactcctcgc
     4441 ccgccttcgt catcgcgatg aaaaggtccg agaagatgtt gcggtgcgcg gccaaggcct
     4501 catggaaggc ctcgcccttc ttcacgcgct cggagaggtc cacgacgatc ttcttcaggg
     4561 acttattggt cgactgccgc tcgatgaccg agagggcgcg ggagagcgag aggcccgcgg
     4621 tgagcatggc ggagaggttc ttggtgaacg tgatgcgctc ctccatcttc accccgctgc
     4681 cgatctgcac ctccgcgagc ttcgcgagga agggcggcat cccgccgccc tcgtccaccg
     4741 acacgacggt ggcgccctcc ttctcggcat cggtgtacac cgcgaaccga gacggcgcgt
     4801 ccatcgtccg cttttcctcg gagccgtccg cacgcttcag cgtcacgcgg tacttcatta
     4861 tgcaaaaatc ttagcacgca caaacgttcg gcccgggtcc ccgcgaggga gaaccgggcc
     4921 aacgcgaacg tcacttcgct gactcagcga gcgaatgccc caccacgatc tggacgaacc
     4981 aggtgaacgc gagcgggacg cagccggcga gggccacgag ccagcacacg ctgccgatga
     5041 ccagcatctg cggcccgtac gaggccccga tggtgccgaa gatgaggccg acgaagaaca
     5101 ggccgatggt gaatgcggcg atcggcacga gctcggccct cgagatgtgg agcagcagga
     5161 gcgcgatgag cgcagcgagc acgcctgccg ggaccacatg cggcaccttc atcaggtacg
     5221 gcacgtcgcc gagcatcgca tgcgacgcga gcccgaccac gatcgcagcg acgatcgccg
     5281 aaacagtcat caaggatttc atggaacatc ctcccagctg cagagtgcag ctctgccgta
     5341 tttatacctc cattacgcac gcgcatcgct cgctatcccc gatatatgga ctgaccgcct
     5401 tccctggggt ggaaaggcgg tcggggtcag gcggcacgag ccgtgacatt gaggacgatg
     5461 ttgtcctggt gatcgaggac caggtagaca tggtgcgcga aggacttgct gaagcttcgg
     5521 gcgagtgcga gcgcctccat aagctcgcag ggttcgctcg tcgccactag tccgccgtgc
     5581 gtgtcgttca cctcgccgac aatgcacttg ccgcttggta cccgccgtaa ttcactcaac
     5641 agggcagtac ccctgtatga tctccttact tcactctata cccagcaggg ccttcgggac
     5701 agcgctaggc actatcggtc accgcgcgca gcacctcctc gatggaggtc gtgcccttgg
     5761 cggccttgta gaggccgtcc tcgagcatgg tgagcatgcc ttccttcctc gcctgctcct
     5821 cgatctggtc cgaggtcccg ctatggagga tgaggtcgcg gatcgccggc gagacggtga
     5881 gcacctcgtg gatgccgatg cggcccttgt acccttcctc gcactcggtg ctcggcgtgg
     5941 gcttatagaa cgggagggta tcgagcttca cggtcgtctt cacgagcttc tcgtcgtgga
     6001 gcgccttcag cgcggcctcg aacttgtcct catgcgcgac cttcgcgcgc tcctcgctat
     6061 ccaaggtgta ctgctccttt ccttcgcaga gccttcggac gagccgctgg cccacgacga
     6121 cgcgaagcgt cgacaccatg aggaacggct cgacgcccat atcgatgagg cgcgggatcg
     6181 cgcccgaggc tgagttcgta tggatggtcg agagcacgag gtggccggtc aaggccgcat
     6241 tgatcgcaag cgccgccgtc tcgccgtcgc ggatctcgcc caccatgatg atgttcgggt
     6301 cctggcgcag gagggcgcgc agcccgttgg cgaacgtgaa cccgatcgcg ggattcacct
     6361 gcgtctgatt cacgcgcttc atctggtact cgatcgggtc ctcgaccgtg gagatgttca
     6421 cgtccggcgt attcagaata tcgaggatgg tatacagggt ggtcgtcttg ccggacccgg
     6481 tcggccctga cacgaggatg atgccggtcg tcaggcgggt cgcggtatgg atgcgctcca
     6541 aggtctcgcc atggaagccg agcgcctcaa gcgagaagcc ctcggcgccg ccctcgcgaa
     6601 ggaggcgcat cacgaccttc tcgccgtagt aggtcggcag gagcgatacg cggaaggaca
     6661 ccgcctccga ctccgtctgc atcttgaaac ggccgtcctg cgggagccgc ttctcgtcga
     6721 gcttcaaatt cgccaagacc ttgatgcgag cgaccaggcc cgcggccgcg ttcttcggga
     6781 gggtcatggc gtcatgcagg acgccgtcga tgcggtaccg gacgatgacc tccgtctccg
     6841 agtgctcgat gtggatgtcg gaggcgtcct ggatgatggc gtggcgaagc agcgtgtcca
     6901 cgatgcgcac gatcggcagg tcctcggcca tcttcttgag gtcctcgccc gagacctcct
     6961 ccccgcccgc ctccttgatg aggttgagct tcccggcctc ggtggagatg aggtcgccga
     7021 actcctcctt cagcgacttc tggtattgca cgaggaccga cttcagagat tcatcgtccg
     7081 taaggcgggg gaggatcttc aaccccgtct tcttcttcac ggactcgatc gaggcgaggt
     7141 cttctgtatc gagcatggcg atctcgaggt cgttgccgct cttcctgaac gcgacgatgg
     7201 agtgcgtgcg ggcgatgggc tcggggatca aggagagcac gtcgagcggg atcttgcggt
     7261 ccttcaggtt cacgaagggg atgccgagca cgtacgcctt gatgcggcgg agcgcgtcag
     7321 ccgagagcgt cccacgcgag acgaggatat cgccgatgga ttggccgcgc tcctccgctt
     7381 ccttcgcggc atcctcgacg tccttccggg caacgagtcc ggaatcgagg atgaactctt
     7441 tgagttcgct ttctgatacc tgcatagcta cgtgctaaaa gtatagcagt cgctgctcat
     7501 agattgcgcc acatcacatt gaaaagttcg gtgtgcatcg tgatgatgct cttgttttca
     7561 atcgagacga gtatgcttgg cttcagcatc tgctggatga tcaccttacg gggcgtgacg
     7621 acgaggttcg tatctacctt cgagtccgcg gggagcattt tgatggtctc ctggaagttt
     7681 gtttcgtgct ccttgtagcg ccgtgcggtt gacgaatcga ccagcatcat ccggatacga
     7741 agcttcttcc ggttgcgctc gcggatgaat tccgtcgcga agctcccgtt catgtcctcc
     7801 caccggtccg gatcaccgac gacgaggaac tcgtcatcgt gattaagccc ctcgagcatc
     7861 tcgaagtatg cgttccggat cgcgcctacg ccttcgtagt acttgatgaa ggattctccc
     7921 cgcttgaggt cgaactgggc ctcgagctcg ggcaggagat cgaggagcgc tccgcgtcgc
     7981 tgctcgagca tgctctcgag cttcttcggg tgctcggccg catagagctg cttgaacccg
     8041 cggacgtcgg tccgaacgag acccttctgc ttgaggctct caagaacgtt ataaatcgtg
     8101 gttcgcttca ccccagaggc tcgagaaatg cttagaaccg ttgccgggcc gagcgaaagc
     8161 atggcgatgt ataccgccgc ctcgttggcg ccgaggccaa gaccctcaag agacgcagta
     8221 atttcggtac tttttgccat ggtggagcat atgtgtcgag aaattcgaca acatattcac
     8281 tttacatcat atatttaaaa gcagcaaaaa atgcaaaaag tcgacaaata tgacagaaat
     8341 gaccgcactg acctagactg actgcgtcgg gattcccctc cgatgacaca tattccttaa
     8401 ccacgaagct atgaaaacca gctatatcgc aatggccgga cagggtgtgt cttcgcatgc
     8461 cgtcatatcg gcagcccagg gcaagtcctc gtcccccgtc atggccggac agggcgcttc
     8521 ctcgttcatc gccaagcaat aggcctacgg aggccgaacg gacacttcgg gtaggtcata
     8581 gacctgcccg aatccgtata ttttatgcag caaccaacca ttaccttctt cccgggaaag
     8641 ggaatctata gcctacctgc aggcgaagag attgtatccg tccatgcgac ggaagaggcc
     8701 cgcgccatat tcgcaggcga tgcctccgtg cgcggaaaag cgaagcagct ccagcggttc
     8761 tacgagcagc agaacatagc gatcgagcag aaaccggtat tgtccgaaac tcgccgattc
     8821 gatttcggaa cggttgaaca tttcggcacg cgtagctccg cgcacgtatg cgccgtagat
     8881 tggggagcgg ttgaggatgc cctcgcacag atgggatatc acctccgcca gcgtactccc
     8941 atcagcgatg agatcgggag gctcgccccg gtatatctga tcgagggacc agagggagtc
     9001 cttgctgaag gcaagggatt ggatcaggag caggcaaagc gctccacgct tggtgaagcc
     9061 gtagagcggc tcgcagcacg tggtccaagc gaacgagtga gggctcaacg aggtacggat
     9121 acgtaccaga atctttatcg cggcaaagat tggggtctcg gcacactcgc cggaccacga
     9181 gatgcattcg attctcagat accgagcgaa tggctaccgg cgcggaacat ctcccgcgac
     9241 gagacagcgt tcctgcctgc ggagctctcc tattttgaat acgtgccgga aaccacgcgc
     9301 acccgtctat tctcgctgca tcacaccatg ggccttgcgg caggctcgtc ccgtgaagac
     9361 gcggccttga gcggtatctt cgaggtcatc gaacgtgatg cgtactggat caccatgcgt
     9421 tgccgcatga atcttccgga tattccgctc gaagcggtcc gggacctgga cccgaaggtc
     9481 attgctatcg tgcaggcgct cgagaaacgt ggattcaagc tcagtgtgaa ggacctgtcc
     9541 ctcgattggg gtgtacctgt ggtccatgcg gttctggttg atacgcaggg tggtatccct
     9601 gcgttcgcgc acggaacggg cgcttcattc tcctgggcga ccgcggtcgc ccgagccgta
     9661 tgcgaatcag tgcagatgta cgaagggctc tctgcggtca tgaacgacga atggaagtgg
     9721 gaggacgtgg tgagcgttgc aggcgtgctc gggaatgcgg cgtacgcctg gtccgacccg
     9781 ctcttccggc ctcatatcga gcatctcctc gctccctccg atcaaccatg ggacggaagc
     9841 gaacctgcat ggtctgtgcc agagttcctc gctgacttaa agcagcgtgg tcatgaggtc
     9901 attgtcgctg atcttggttc agactatggt ctagaggttg tgcgcgtatt catcacgaat
     9961 acgacgcagc cagatccgcg acttgagcga atcagcctgc ggcttgaagc gtggcgaaac
    10021 cgtcttgggc ttgagcgagg cctgtatacc gacccgatcc tgacctaatt attcagaaga
    10081 cccttcaggt gttcgaagat gggccgcagc tcctcctcgg agagcgtgag ccgcggattc
    10141 gacttccgcg tggtgggtgt gaggtccacg tactcgcggc cattgaatgt gagcggtgca
    10201 tcgagccgag gcgtgaaatg catgtgccca tgcccgcgat ggtgcttcgt ttcgttcccg
    10261 agccattcga cgttgagcgt acggagcggg agccacgagc gcatcgcgtc cgtgaactcc
    10321 ggaatgatcg tatacacgag ctcatggagc tcctccttcg tgagctcagc gaggctcatc
    10381 tggtccacat ggcgcttgag ccacgcggtt gatcggccgg gcagctcctg cttcgtgatg
    10441 ttgagccgca gcgaccaatg ttcgtattcc ttcagggtcc agggcgcgta caccgggctc
    10501 cggaggatgt cctcgagctc ctcgggggtc gcgcggcggg agagatcgtg ctccatatgc
    10561 cccattctag ccttcattga ctgttccgtc ctatatatgg tatgttttcg ctatctgaaa
    10621 tgtccgcacc cgcgggcctt tctttttaag ggcgacaatt gaatacacaa ctaaataccg
    10681 gagtatgttg gatctgaaga cgattaactc ggtgctggga gagttcgagg agcggggcat
    10741 tacgaaggcg acgatgatcg acgccatcga gagcgcgatg gcgaccgcct acaagaagga
    10801 atacggcaag cgcgggcagg tcatccgcgg caagctcgat atggataccg gcaccgtatc
    10861 cttcgagcag gtgaagacgg tggtggacga caccatggtc cgcttcctcg aggagggcga
    10921 ggagacgaat gagctcccgg cccaccaccc ccaccatgac cgcgaggagg aggtcgccga
    10981 gggcgagctc ccgcgcttcg acgccgagaa gcacatgctc atcgaggacg ccaagcgtat
    11041 caagcgcgac gtcaccttgg gcgaggagct catcttcccc ttggagcccc aggacgactt
    11101 cggccgcatc gcggcgcaga ccgccaagca ggtcgtgatc cagaagatcc gcgaggcgga
    11161 gaagctctcc atcctcgacg agttcgcgca caagaagggt gagatcgtct cgggcatcgt
    11221 gcagcgcatc gagcgcggcg cgatcttcgt cgacctcggc cgcgcgaccg gcatcatccc
    11281 ctacgaggag cagatcccgg gcgagcgcta ccgcaccggc gagcgcatcc gcgcctacct
    11341 ctacgcggtg gacgagggct tccgcggcgt atacctcagg ctctcgcgca gccacccacg
    11401 cttcctcatc cgtctcttcg agatggaggc gcccgagctc gcgtcgggct ccatcgaggt
    11461 gaagggggtc gcccgcgaac cgggctcccg cacgaagatc gcgatcgcct cggtcgatcc
    11521 gcacgtggat ccggtcgggt cgcttgtcgg ccagcgcggg gtccgcgtct cgaccgtcat
    11581 gagcgaactg ggcggcgagc gcatcgacat catcgagtgg tcggaggacc aggcggcctt
    11641 catcaaggaa tccctctccc ccgcttccgt gctcaccgtc gacctcgacg agacgaacca
    11701 ccgtgcgaag gtgaccgtct cggaagatca gcagtccctt gcgatcgggc gcggcggcca
    11761 gaacgtgcgg ctcgcggcga agctcaccgg ctggaacatc gacatcgagt cgaccggcgg
    11821 ccccgtgggc ggcgcaggcg ccgatgagat ctccttcgac aagcagaagg cgcacgactc
    11881 gaccatcgcc gcggaagcgg agatcgaggc cgaggaggcc ggcgtcgagg acagcccgtc
    11941 tatcgaagcg ggcatcgccc cgaacgagaa cgagtccgtc atcacctccg aggaagatgc
    12001 atcggacgat accggcgcac tcacggtgga ggatgaaacg aagctcggcg acgacggcac
    12061 tcccgtgaat cccgatgatc gggacgtcac gagcgagagc cagagcgcga atcaggcata
    12121 attacctcta tgaaccttct tcacgacatc cccgcaggct cgaaggacga gatgaacgtc
    12181 attatcgaag tctccaaggg ttcccaaaac aagtacgaga tcgacaagga aaccgggctc
    12241 atcaagctcg accgcgccaa ctactcggcc gcggccttcc ccgccgaata cggcttcgtg
    12301 ccgcagaccc tgtgggacga cggtgacgcg ctcgacgtgg tcgtactcac caccttcccc
    12361 atcccgaccg gcgttctagt taaggtccgt cccgtcgcgc tcatggagat gatcgacacc
    12421 ggcgagaacg attacaaagt catcgccgta ccggtgaagg ataagcgctg ggaggacgtg
    12481 cgcgatctcc cggatctcaa taagcacaca ctcaaggaga tccagcattt cttcgagacc
    12541 tacaagcagt tgaagggtga ggaggccgtg gtcacgatca acggcttcaa gggcaaggcc
    12601 gacgccgaag cagcgttcga gcatgcccgc aaactgtacg aggaaaagaa gtaatcctcc
    12661 ggaaaagaaa gagcccgcgc gcagcgcggg ctctttcttt tccccgttcg cctaaagcgt
    12721 tgccgctcgc tatactccgt acatggaacc atcaggagga ccgataccgc ttgcgccgaa
    12781 ggaacccgta ctgccgcagc ccatcgtacc ggtcgcggaa ccgaggcccg tgataccgcc
    12841 tccgccgcca ccgcgacctc ttccaccgcc catccctcca cccccgattc ctccaccggt
    12901 tgtgaaaccc gcaccggcaa ccccgaaggc caagggcggc tggggcgcca tcatcgcgat
    12961 cttcctcatc gtggccctca tcatcaccgc cgcgttctac agctggggcg aacggctcgc
    13021 gaccgagcaa tcgctcgagg aaatcgcgaa atagccgcgg cgtgcgccgc ggctacgttt
    13081 ttggtaccat gcgcacacta tgaacaacgc accccgcgca tccatgctcg tcccgatggt
    13141 cgtcgagaaa tcgccctttg gcgagcgcgc ctacgacatc tattcgcgcc tcctcaagga
    13201 gcgcatcgtg ttcctcggtg gcggcatcga tgacgatatc gcgaacctca tcatcgcaca
    13261 gctcctcttc ctcgaatcgg aagatccgaa gaaggacatc tcgctctaca tcaactcgcc
    13321 gggcggctcg gtgactgcgg gtctcgcgat cctcgacact atgaatcatg tgaagcctaa
    13381 cgtctccacc gtctgcgtgg gcatggcggc atccgccgcg gcagtcatcc tctccgccgg
    13441 cgagaagggc aagcgctacg cgctcccgaa cgccgaggtc atgatccacc agccctgggg
    13501 cggtgcgcag gggcaggcga ccgatatcga gatcaccgcc aagcacatca tcgcgacccg
    13561 cgagcgcctc aataagatcc tcgcgaagaa taccgggcag ccggtcgaga agatcgagaa
    13621 ggacgtcgat cgcgactact ttatgatggc ggacgaggcg aagaagtacg ggatcatcga
    13681 tagcgtgtat aagaaataga tgagtgaacc acaacagccc gcggaattcc gcgggctgtt
    13741 gtggtttcta ggatgggccg aggagccatg ataccctgcc cgtgtcagtt gcgaccgaac
    13801 gattatccga ccgcattccg gctgaccctt aacttgctct catccgtttc accgaagggt
    13861 gattcggcac actggtatgt ccatcaaatt gatattggcc ctgcttgccc tgtcggggct
    13921 cgcgggcatc gcgctcgggt acgtgctgcg tctcctgatc gcgctcgggc agcgcggctc
    13981 gctcgagctc gaggtgaaac agaagctgct cgaggcgaag gaacgtgcga accgcatcat
    14041 caccgaagct gaagcgcgtg cggaaaaact cgaggctgag atcgcgcttc cgttgaagac
    14101 gcgcgaggac gcgatcgcaa agtccgcgga acggctcgcc aagcgcgagg aattcctcga
    14161 cgaacgcgag cgcacgctcg cgagcgaagc agaagacgtg cgcaatcgcg agaacgaagc
    14221 cgcgaacttg aagcgcgaag cggagaagct tctcaaggag cgccgcctcg agctcgtgcg
    14281 catgggccgc ctctcggaag cagaggcgcg taccctcctc atgaaggacc tcgaagagac
    14341 cgagaccgag acgctgctcg cgcgtctcca gaagctcgaa gcgcacggca ggaagcagct
    14401 cgaggagaag gctcgcgcga tactccttgc gaccatccac cgcctcggct cctcggtgaa
    14461 cgcggacatc atgacgcttt ccgtcacgct gccgtctgag gaggccaagg gaaagatcat
    14521 cggcaaggag ggccggaaca tcaagacctt cgagcgcgcc accggtgtgg acgtcatcat
    14581 cgatgatgcc cccgataaga tctccctctc ctccttcgat ccgctccgcc gccatatcgc
    14641 taagctcgcg cttgagaacc tccttgagga cggccgcatc cagccggcga agatagaaga
    14701 gaccgtcgag aaggccaaga agcaggtgca ggacatggtg aagcagaagg gcgaggaggc
    14761 cgcgtacgag gtcggggtcg tggggctcga cccgaggctc gtcgcgctct tgggtcggct
    14821 ccatttccgc acgagctacg gccagaacgt actccagcac tccatcgaaa tggcgcatat
    14881 cgccggtatg ctcgcgaccg agcttggggc ggacgtggac gtcgcgcgcg ccggcgcact
    14941 cctgcatgac atcggcaagg ccgtggacca cgaggtgcag ggtacgcacg tggagatcgg
    15001 ccgccgcatc ctcgagaagt tcggggtggc gaaagaagtc atccaggcga tgcagtcgca
    15061 ccacgaggaa tatccgtatg aaacgccgga gtcggtcatc gtgcaggtcg cggacgcggt
    15121 ctctgcgggt cgtccgggcg cccgccggga cacggtcgag cggtatctcg agcgcctcgg
    15181 ggacctcgag cgcatcgcca aaggcttcga gggcgtcgag aaggtgtacg ccatctctgc
    15241 gggccgcgag atccgcatct tcgtgaaccc gggcaaggtc tcggacctcg agatgcaccg
    15301 cttggcgcgc cagatcgcgg agcgcgtgca ggcggagctc aagtatccgg gtgaaatcaa
    15361 ggtgaacgtc atccgggaga accgggtggt ggagtttgcg cgctaatggc tgcattgaca
    15421 ccactgctga aaagtcgccc gctgggcgat ttttcactat ccacggccaa attcctcagc
    15481 tattgcgtaa aatggctcat ttcctatact ttgcgcgtag ccctctcggt agggcttacc
    15541 taagccctac aagggattac ccctaattac gcgacaaaca ccatgaacaa agcagatatc
    15601 gcagcaaagg tgcaggagac tctcggcagc accaaggcgg acgcagagcg cgccgttgag
    15661 accatcatcg aggcaatctc gagcggcctc aagtccggtg acgaagtctc gatcgccggc
    15721 ctcggcatct tcagcgccaa ggccaggccc gctcgcaccg gccgcaaccc gcgcaccggc
    15781 gagaccatca agatcgctgc gacccgcacc ccgaagttcc gcgctgcaaa ggcgctcaag
    15841 gacgcggtta agtaaatcgc tcggttctag agcaaggagc cccgggaaac cggggctttt
    15901 gcttacacta ggtcccaaag attgcggaac acttcgcgct ggtcatgcgc aaggagcctc
    15961 gaatggatct cgaccatgta atgcgggtgt tgccgggtgt tgatcataat gacgtagtcg
    16021 ccgcatatcc aggtgctcga gataaaattc gtcgcttctc cccaatattt catatcccgt
    16081 cgctcatact tccccatcat gcggcgctcc gccgaggaaa ggttcgtgag gagcttcaca
    16141 tagagctcag gcggcgcgat gctccaccaa tagtggagtt gatcctcata ggcctcaaca
    16201 agagttgcgt cctggtaccc ccagaagccg tactctttgg tttcaatcat ggattccatc
    16261 catatcggga agacctgcgg aaagtaggct tcgagttttg attcttcagt gaatttgatg
    16321 cggggcaccg gataggtcgt ctccgcgtta accatggaaa cttcctccgc gagcttcttc
    16381 aggagttgct ggcgctcttc agagcgcttc cgttccgtct ccaccgcaag cgcgagatcc
    16441 tcggtcgccg caggacgaaa tacgcgtggg cggcggctgg agtcctctat cagaagtcct
    16501 ttctcgatga ggctccgggc gatactgtat gcggtcgtgc gcttaatgcc ggtcgccttc
    16561 gcaaggcgcg ccggatccag ctcgcgggct ctcaacactg ccttgtagac cttggcttcc
    16621 cgcgtattga ggccaagcga aatgaggagc tgttcgtcca tatgctaagg ctaactgttg
    16681 tcgaatattt gtcaacattt gttcgacata tgttgaccaa cttgacacct tttgctttta
    16741 tatgcacaga cagcgatgag cgctgatcaa acaacctcat ggaggacttg gtatgcgtat
    16801 cacgcttgct cttcttcttt cggcgatgct tctcgggtcc gcccagaacg ccctcgccga
    16861 ctgtaccgac accctctacg ggaaggccgc gagcggttac atcctgaagg acgtcggcgc
    16921 ggaggcttcc gaccagccgg tcgtccaggg cggcgcgacc cgcacctgcg gcagactctc
    16981 ctacgacctg tgggtgagcg gcggtctggc gggcgacacc gcgaacgaga tcgacctatc
    17041 cgtctactac gacgaccagg tcggtcccct gaaggtccag ctagcggcac agtacttttt
    17101 cgtcagcctc gacgactccc tcggcgagcc gaaggacgac atcgtcgcgg gctacgcgga
    17161 cctgagcctt cccgtcacgc gcggcaggct cacgatcgcg cccctggtgc ggttcgtgca
    17221 gctcgtcggc gtgaaggacc tgccgaacga gggcatcgtg cagcccggcg tgcgtggatc
    17281 tttcgcgctc gacgatcgta cgacgctgtc ggccgacatc cggcatccga tcagctcgcg
    17341 cggctactcg ttcttccggg tcaccacgtc gctgtcgcgc caggtcaccg aacgcttcgc
    17401 ggcgcaggcg ggctgggagg acacggatca gacgccttcg gtcttctggt tcggcggcaa
    17461 gtacaacttt tagggagaaa ggagcattcc ggggcgatac atcgcccctc ccattcaggt
    17521 gcggatatgt tccgcacctg ataggagaaa aatcatgcgc gtgttctttc aagaactcaa
    17581 agagattggt caggaaaaca actgctgggt gtgcggcgcc ctatacgtcc tcatgcaggt
    17641 tggcatgatc gcccttggta tatgggccgc agctaaccgc atatgcccat aggagaggtg
    17701 atatggaaac tggacaccgt ttcgccggga aattgagcga agactacgat ctggtgcggg
    17761 tcatgattcc gcactacgac gaacaccagg acacgatcca cacggcgatc gaggcatttg
    17821 cgctcacgca cacgccgatc gagattctgc cgaccatcga tatcgggtgc ggtaccggct
    17881 tcacggccga gcgcattctc gatgccgatc cgcgcaccac gctcatcgcc atcgacaacg
    17941 aacctgcgat gatggatcaa gcgagggtgc ggctcgagcg gttcggcaat cgtgtcacct
    18001 tttacgagat ggacgcgctt gaggcgatcg gtcgcctgcc ggctcggagt gcggagattg
    18061 tggcttctgg atgcgttttg cataatctcc cgcacgacta tcggtggcgc ctgatgcgga
    18121 aaatcggacg tgtgctggtg cctggcgggc tcttcgtgaa tgccgataag ttcggcctcg
    18181 acgaccctat ggaacacatg cttcaactcg ccgaacagct caagaacatt agcgtgctcg
    18241 acgacatggg gcggtccgat ctacgggaag cgtggcatgc gcattatctc gaggatgagg
    18301 agatcaactt cactgagagc gagcaaaagg cgttgctgcg cgagtcaggc ttctggggaa
    18361 ttaccaccat ataccgtaag cgcctggaag cgaccattgc tgccttcaag aaataagtag
    18421 cagagggagc agctctttac gggcgaccaa gcgtcgcccc tcccattcag tcgcgcatgc
    18481 ctgcgcgact gataggagca agatatgcat tccatataca acaaatctgc ggaggatatg
    18541 agcgaggtgc ggagcaattc cgtcaatctt atcgttgcgt ctacccccta taacgacgga
    18601 acggtgtacg gccagttcgt agacaagact tccctcgagc agtactgcca gatgatggaa
    18661 cgagtaatcg ctgaatgtgc acgcgtcctg catccagaag ggaggctcat catcgaggtg
    18721 gcggatactg cgatcgtgca gggccatttc gtgcagcttg cagccctgtg gcagcaggca
    18781 gcgttgaggt cacagctgca cctgagcgag cggcatataa atttcgttca gacccataag
    18841 ggcaacgaag atgcgactca tggggacgga ttcatctctc cgccagtcac ggcggagttg
    18901 gatgaaacgc actcgaacag ctgccagtgg cttgtgttca ccaagaaatc ttctgaattc
    18961 ctgggtggga acatctacta tcagaactac gtatacgatc ttgaggagca ccactgtcct
    19021 tatacggacg agttttgtag aaccttcttt gagattgggc acttcaagtc tggcgacacg
    19081 gtactggaag tgttcatggg tactgccacg ctgggcctac acgtattaga gcgagagggt
    19141 atatacatcg gttacgaaat tgactcgatc atattcgcgc gcgcccaaca gcggcttgct
    19201 cgataatcga gatccgtgat cacctagccg cgcctactcg gcgcggtctt cttttctgaa
    19261 atacttccta tccttcagct tctccggcat ataactctcc gtgtcgtatc gcttgtatcc
    19321 cttcttgtac ccgaggtcct tcatgagctt cgtgggcgca ttccggatct tcttcggaat
    19381 cggaaggttg ccatgccggc gcacgtctcc ctgcgcttca ccgagtgcct catatgccga
    19441 cttatccttc gctgcgttcg aaagataggc gaccccatgc gcgagattga tggcgcactc
    19501 aggatacccg atcacttcac aggcgcggaa gaccgcgttt gcgaccacca aggccgtcgg
    19561 ttgcgcgagc ccgatatcct ccgaggcaaa gatgaccatc ctgcgcgcga tgaagagtgg
    19621 gtcctccccc gcctccacca tccgagcgag gtagtacatg gcggcgtcgg gttgcgaggc
    19681 ccgcatgctc ttaatgaacg cgctgatggt gtcgtagtgc tcgtcgccct tcttatcgta
    19741 gcgcaggtgc gggtcctcga gtgccttatt gagcgtatca accgatacct ttccatagag
    19801 ggatttcgcg ccctcgagga tggtgagcgc ccggcgggcg tcgccattgg cgtagccgag
    19861 gagccaatcg aacgcgtcgt tggaaagctt gatcttcgcc cgagcgacga tcgcccgaag
    19921 cgattcctct gaaagtggtt cgagtacgaa gacccgggcg cgggaaagaa gcgccgggat
    19981 gacctcgaag gaggggttct cggtcgtcgc accgatcaac gtcagctccc cgctttcgac
    20041 gtacggcaag aggaagtcct gctgcgcctt gttgaagcgg tggatctcgt cgaggaagag
    20101 gaccttgggg cgcagcccca aggtcgggcc cttcccctcc ccgacgatct tccggatatc
    20161 ctccttgccg gccgaaaccg cggagagctc gtagagctcg gccgcgagcg cgcgggcgta
    20221 gatgcgggcg atggtggtct taccggttcc gggcggtccc cagaggatga aggagaaccg
    20281 gtgctgcttc tcgatagcga cccggagcgg cttattcttc ccgaccaaat gctcctggcc
    20341 cacgaactca tcgagggact gggggcggat gcggttggcg agcggttcca tgcctgaagc
    20401 gtacgctcgt agggatgaaa atcaatgaag agtcgggtgc ggggggtgct gctgggtctc
    20461 agctatcagc gcatctaatt cctccggatt agccatattg accggaaagt cgtccccctt
    20521 agtatctcgc aatccacgaa accgctgagc tagtgttgcg ccgtcttcct ccttatccat
    20581 ccacaattta aaggccgcag aagggctggg agcatcggga aatgcgcgta tggccagtgc
    20641 gatctgaagt cgtgggttat tgagatcaac ctcaggcgcg aacgactcgc ctgaacgaga
    20701 gcgatgtact tcgtttttgc gtgtcgaatc aagattacct tccataagga taaggatata
    20761 ccacacacat gctcgagaaa cgaaagcacc cgccgaagcg ggtgctttca aaggagtggg
    20821 ctggcttagt cgtcgatctc atcgactgcg tcgtcggcaa gctcctcggc ctcctcagcg
    20881 tggtcgatcg catcgcggta gtccttgcga tcgaaggcat cgagagcgtc ctcgtattcg
    20941 tcctcggcct catcgaggag gtcctcggca tcatcgacgt cctcaccgtc atcgtcagca
    21001 tcatcgatct cctcccaggc gtcatcgatg gcatcgtccg cctcctcgat cgcgcggcgg
    21061 gcattggcct cagatgcgct cgtgctcgag gaaccgatct catcgacggc atcctcggcg
    21121 ttctcctccg catcgtcggc gaagtcgacc gcgtcatcgt aatcgccgtc attgtaggcg
    21181 cggcgggccc tcttcaggtc gtcgcgggcc tcatcaagga ggtcctcggc gtcacgcacg
    21241 ttcttgttcg cgcgcctggc gtccgaaatg tcatcgcgcg cgtcgtcgat cgcctcgcgg
    21301 gcatcgtcga tcgcctcgcg ggcgtcccga cggtccgcat cattgctgcg attgcggttc
    21361 gagttacgcg tgtcgatgat cggcgtgttc gagaacgggt tggacgggtt gccggcgaga
    21421 gcggagacac ggaagacgcc ggtcacctgc tggccggtcg ccgcctgcca acgcgcaacg
    21481 gctgcctggg tgaggggccc gaagctgtag ccctcgcgac cataggagcc agcgccggtg
    21541 tacgcgactg ggtagcctga cgcgttgagg tagcgctgga ggcagcgcac atcctcgccg
    21601 aaggcgccga ggaagagatc gcgggtgaag acgcactggg tggtctgcgc gaactgggcg
    21661 cttgcggtgg gcgcgaagcc gagcacggca acggcgccga tcaccgcggc gagtactacc
    21721 aaaccgatct tagagattgc atttagcata gtaaataagc catttaataa ttgtttgccc
    21781 tgtacctact acggacaata caacaatacg ctggagaatc aatgaagatg agctttggtg
    21841 cgggatagga gaatcgaact cctgctctca gtttggaaaa ctgatgttct accattaaac
    21901 taatcccgct tcggtaggca caatagcata ccgtacctcc tctgtatttt taggaagcaa
    21961 cagtgtagcg tttaggtatg cctgccctcc aaggcgttct ctccgccctc tcggcgctcc
    22021 ttatcaccct caattcccta tatgggactc cgatgagtag taccaccccc gaggtgaccg
    22081 gcatcttcgg cccggccgcg acctcgagtg cgccactccc cccgaagcga tctccctcta
    22141 cccttgggcc gctccttccc caggccccgg ccatcgggct ccccggaacc cttgcgacgc
    22201 ttcggggcgc catcgtgaac attgtctgta cctcaaagga ctcgcgggtc cgctctatgt
    22261 ccggcagcgg ggtcatcttc gacccgaagg gtctcatcct cacgaatagc catatcgggc
    22321 agtactacct tgtccgagac gcgctcccta agggcacgat tacctgcacg attcggaccg
    22381 ggaatccggc ccagaccgct taccgcgccg agcctgtgta tgtctcgagc cagtgggtgg
    22441 ccgctaatcc gcgcaccctt gtgaccgcag gggcaagcgg caccggcgag cacgatttcg
    22501 ctgtcctcgc gatcaccgag agcgcaactc ccctcccatt gccgggcagt ttcccctata
    22561 tcgcgctcgg gaagggcgta cccgtggagg gcgctgaggt cgcgcttggc acctatggcg
    22621 cccagtccct ctcttcgcgc cagatccaga attctctctt ccccacgctc gtcaccgcga
    22681 aggtgaggac ccgcttcagc ttcgataacg gtcagatcga cctcatcgcg ctcactggtt
    22741 cagcagcctc ccagcagggt tcttcgggcg gcgcggtcgc aaacgcaagc ggcgagctca
    22801 tcgggatcat caccacgagc acccagaccg gggaattctc gagccgcgag acccgggccg
    22861 ttaccacagc tcacatccga tcgagcttca agcaggatag tggcgaggac ttcgatcggt
    22921 acttcaagaa cggctataag gcggacttcg ttgacgtctt caccgacgat tccgaacgcc
    22981 ttggcacctt cctcgtgaag cagctggacc gctaatccgt gctattctca ctccattcgg
    23041 ggtgtcgtat accggtagta cgcctgcttt gggagcaggt agacggagtt caattctccg
    23101 caccccgaca aacgagtgca taattgggtg ctttagaaat accttgtatt tgctgagttt
    23161 tttcgaagca tgaaggacaa ttcattgttt gtcctatgtc ctttatcccc actgtcccct
    23221 aggatgtcca cagttatgaa caatgcaaaa cggccccttc gggccgtttt gcattggtgc
    23281 ggatagggag aatcgaactc ccgactcgtc cttggcaagg acgcgtttta ccactaaacc
    23341 atatccgctt cgcgaggcat catagcacag ttacctcccg cctatccagc aattatctcc
    23401 ttcggaagtg gccggaggtt gtaggtggag gccatcgaat atccataggc gcccgcgagc
    23461 cggatcgcga gcacatcccc gatcttcggc agcggcatca tccgcttcac ggcgaagaga
    23521 tcgccggatt cgcagatgtt cccggccacg gtgaccggga cctgtttccc tcccttccgg
    23581 ctgaggttct cgatggggtg ataggagcca tacatggccg gacggacgag atgattgaag
    23641 ccagagtcca ggccgacaaa ggtatgccga taggttatct tgatatcggt cacggtaacg
    23701 agaagcgtgc ctgcctcggc gacaaggaaa cggccaggct ccatggcgaa ctgaattcct
    23761 cttccctgtt tcttagcaaa cccctccatt tccttctcaa gtgccttgcc aagtgcggcg
    23821 agatcgagct gatcttcctc aggcttgtac ggcacgccaa gcccgccgcc aaagtcgatg
    23881 gcgatgacgt ccgggaagtc cgcagcagcg gtaatgagcg ctcgcgcagc cttaacgtac
    23941 agttttgaat ccaacacgtt cgatccaatg tgctgcatga gcccggtaat caggagcccg
    24001 tacttcttgg cgattcgctt agctgccgga atatccttct tatcgatccc gaatttgctg
    24061 tcgggccctc cggtgatgac gtgcgcatgg tgtccacctc ctatcccttg attcaagcga
    24121 agcgatatct ctttcccgag ccttcccttc ccccagagct cgagctgggt gagcgagtcg
    24181 aggtagacgc gtccggcata cctggctgca tagcgcagct cggattctgt aaggttagag
    24241 caggtgaagc ttataccatc cttagcaaag cctgccgcat atgcgcgttc gatctctccc
    24301 cgactcaccg cctcgatccc gattccggcc ttccggatga tcttcaggac ctccagattg
    24361 ctgttcgcct tcattgcgta gcgaacggcg acacctggga agtggctagt gagcttcttg
    24421 gcctgtgctt ctatcgcgct ccggtcgtag acatagagcg gggtgccata cttcttagtg
    24481 agcgcaagga acgcggactg cttcatggtg ctcccgccag gactcgaacc tggaacgacg
    24541 gttccgaaga ccgttgtgat atccatttca ccacaggagc gtgtagtagc acgatactac
    24601 aggctaggag attggccacc agcgtactag tatttttctg atgcgcttcc ccatcccgct
    24661 tgaggtacaa gccgtaaccg acggcctccg gaaagagggc tttgaggcct acctggtcgg
    24721 tggttgcgtt cgcgacctcc tccttaatcg gaccccaaag gactgggatg tgaccacgaa
    24781 tgccagaccc gaggatatcc agcgggtctt cccggactcc ttctatgaga acgattttgg
    24841 gacggttggg gtgaagaccg gggccgagga cccgaagctc gccgtagtcg aggtgactcc
    24901 ctaccgcacg gagaccggat attcggacaa acgccggcct gacgaggtca tcttcgggga
    24961 gagtcttgag gacgatctcg cccgccggga cttcaccatt aacgccattg ccctcgagga
    25021 ttctaaagga cagctcgttg acccttataa aggacaaaag gacatcgagg ctaaagtact
    25081 gcgtacagta ggtaatccgg ctgatcgctt cgaagaggac gccctccgcc tcatgcgggc
    25141 agtccggctt atggcagagc ttggcttcgc ccttgattcc gagacggccg aggcaataag
    25201 ggttaaatcc gggaatctga ggcacatttc acgtgaaagg attcgggaag aattcgtgag
    25261 aattgtggag tcaaaggaac ctatgatggc cctcgttttg gctcatcagc tgggaatact
    25321 cgagtatatt gctcccgatc tcatacgggg cattggggtt gatcagaacc aggcccatag
    25381 ctacgacgtt ttcgagcata atatgcgcgc cctccagcat gctgcggaca aggactggtc
    25441 gcttgatatc cggctcgctg ctcttttcca tgatgtatct aagcctgaaa cccggcgctg
    25501 gtccgatgag aagaaggact ggacattcca tgggcatgag gtagtaggcg gcaggacggt
    25561 taggaaggcc cttgaggatc tacggttttc acgcgaaaca atcgaaaagg tgagtaaact
    25621 ggtccgttgg cacatgttct tctcggaccc tgatcagata agcctgtccg cggtccgccg
    25681 aatgatcgtg aatgtcgggc ctgagaatat tgaggatctt ctgaatctac gcatctgcga
    25741 ccgtatcggt accggccggc ctaaagagca gcccttccgc ttcaggagat ataaggcaat
    25801 ggtcgaggag gcacttcggg atcccatctc cgtcgggatg cttaaaaccg atgggaaccg
    25861 cctcatggaa cagtttcatg tgaaaccggg gcctaggata ggccatattc tccatgcgct
    25921 cctcgaggag gttcttgagg atccttcaag gaataccgag gaatatctgg atgctaggac
    25981 gaaggagctc ctatcgctag gagaccagga gctcaaggaa ttgggggagt cgggaaagaa
    26041 gagaagggag gtcgaggagg agcgggagat acaggaaatc cttaaaaagc accatgtggg
    26101 gtaggttaca cgtgaaatag aaagggcctc gttcttagct tagaatcgga ttccgagcta
    26161 aaaacgaggc ccttacttac ttttggctac tttgcgttct gcgaggtgca gaacaagaaa
    26221 atggagctgg ggcttttgtt ggaattctgc tggggaaagg gcagaaacgc atttcgcatg
    26281 atttatgttg cgtttccacg tggaaccggg cctgacccag ttttataggc ggagatacgc
    26341 tccgaagccg ttggacggcc ccgaaccttc cgtgagacgc acgcacagaa agagctctca
    26401 aagatcaacc gaagcgcgga gaggccgcgc atcatggact gatccgcaag ccctccccgt
    26461 ctgcagataa tagtagggtt tataaaggac aacgtaaagg acatacgtgt gcataacttg
    26521 tcctttatgt cctatagctc tatagaaaca gggcagtggt ccgagccgag tacttccgga
    26581 tggatgccgg cctgctttac atgtctcgcg agcgtcttag aagcaaagat gtaatcgatg
    26641 cgccagccga cattgcgttc tcgtgcgttg ccccatgggc tccaccaagt gtagtgacct
    26701 gccccttgtt tgaagagccg gagtgtatcg acgaatcctg ccttgaggat ctggtcgatg
    26761 ccttcccgct cctcgtccgt gaagcccttc ataccgacgt tcgcatcggg acgggcaagg
    26821 tcgtccgggg tatgggcgac gttcaaatca ccgcagaaaa taacgggttt ctcctcctcg
    26881 agccgcttca tgtattcaag gaaggcgggg tcccattgct tatgccgaag atggagccgg
    26941 gagaggtcgt ccttggcgtt cggcgtatag acggagacca cccagaagtc gaacatgtcc
    27001 gctgccacaa tgcggccctc ctcattgggg tctccgtacc cgtcgtctgc gagcccgtat
    27061 ttcttggcga tatcctcggt catgccgtag accacgtcgt ttggggtcat cttggtgaag
    27121 atggccgtcc cgctgtatcc cttcttattc ttggcggaat tccaatattc ctcgtattcg
    27181 ggtaggtcta tgggagattg ttcctgctcg gccttggtct cctggaggca gaggatgtcg
    27241 ggcttatgct tcttaatgaa cggtacgaac aggtccttgc ggtggaccgc ccggatgccg
    27301 ttcacgttcc aggacatgat gcgcatagcc gcatagtagc aggctatact ctcggtatgg
    27361 aagacatccg gcacgaggtg gtcatcaccg cgatcatcgt gaaggatggg aaatatctca
    27421 tcacgcgccg ctccccgaac aagaagcggt ttccaggcat gtggacggtg ccaggcggga
    27481 agcttgagcc taaggactat ctctccatgc ccaaagacac taaggactac tggtataacg
    27541 tgctcgagcg cgtgctccga agggaagtcg aggaggaggt gggactttcg ataacgaata
    27601 tcgagtacgt gacgagcctt gcgaccgtgc atgcagacgg cgcgccatcc ctcgtcatct
    27661 cttgcatggc cgattacgag agcggggagg tgcggctcca ggaagaggag actgaccagt
    27721 atgaatgggt gacgctcgag gaggcgaggg gctacgagct catcgatggt atctacgacg
    27781 agctcgcgat ggcgcagatg cgcaggaggg gagagcggat cgagtggcag cgcgcgtaag
    27841 tgctacttcc ttgaatggaa ccgcttatgt acttctcgta agtgcgcatc cgtcacatgc
    27901 gtatagatct gagttgtatt gatggaggca tgacctaaaa gtgcctgaac gctgcgcaga
    27961 tccgcgccgt tcgagaggag atcggtcgca aacgaatggc gcagcacgtg cggcgtgacc
    28021 ttcttcgtaa tgccggcctt ggtcgcgtag tgcttgatga tgagctggac gccccgcggc
    28081 gtgagcctcg tcggggtctt cttcgaatcc ttcccgaccc gcgggatgtt cacaaagagc
    28141 gcctcctcca tgtccttgcg cgccttcata taggtacgga ccgcttcctt gctcgcgtcc
    28201 gacaggaaca ccacccgcac cttgtctccc ttgccgcgca ccgagaggtc gtcgcgcgag
    28261 aggtcgatat ccgaattgag cgcgcagagt tcggaaacgc gcagtccggt ggagaagagg
    28321 agctcgagga tggcgcggtc ccggagcgcc ttctcctcgg tcccgtccgc ggccttgatg
    28381 agccggtcga gctcggcgct cgagatgagg tcaatctgcc gctcggggag cttcgcaagc
    28441 tcgatgcgct ccggattgag cccctcgatg ccgcgcttgc ggaggaactt gaggaaggcc
    28501 cgcagcgcga tgaggtagta gttctgggtc cggcgcttca tggtgcccga ggttcctggc
    28561 tggcggttca gccacaaccg gaattcccgg atccgctcct cggtgatatc cgagacttcc
    28621 ttgatctcca tctgcgtgaa gaaccggtcg aggtagtgct cgtaattttc gatggtcttg
    28681 accgcgcgac cgcgctcgat ctcggtgtat tcgaggaact ggcggagcat cgagcgagca
    28741 tccataggac cagtataccg ctccgttgtg cgaagtatcc ggattgctat actttccgta
    28801 tatggaacta ctcgtcattc tcccgatcgc catcttcctc gctgtcgtgt tcatccgcca
    28861 ggtgaaccaa tacgagcggg gcgtccgctt cactatgggc cggttctcgg ggatcatgaa
    28921 tccgggctgg cgcatcatca tcccggtctt ccagggctac caaaaggtgg atatgcgcac
    28981 caaggcggtg gacgtcccgg accagaacgc catcacccgc gacaacgttc cggtccgcgt
    29041 gaacgccgtc atctactaca aggtctcgga cgccgagaag gcgatcatcg aggtggagga
    29101 cttccgctac gccatttccc agtacgcgca gaccaccatg cgcaatattg tcggcgaagt
    29161 gacgctcgat gaactcctct ccggccgcga taagatcgcg gaccgcatcc gcgagatcgt
    29221 ggacctcgaa accgacgcgt ggggccttaa ggtccagaac gtggagctta aggatgtgag
    29281 ccttccggag gcgatggagc gcacgatcgc taagcaggca gaggccgagc gcgagaagcg
    29341 ggcggtcatc atcaattcgg agggtgaagt cgccgctgcc gagaacgtgg cgaaggcggc
    29401 gcaaatgctc tcgcagattc cgggtgcgct ccacctccga acgctccaat cgatcaacga
    29461 tatctcctcg gaccagtcca ataccgtggt cttcacggtc ccgctcgaga ttctgaaggc
    29521 gctcgagggc ttcaagcggg ggtcctagcg gaggccgctt cataccccac aacacttgca
    29581 attattatca agatgtggta aggtgtggtc aatgcttacg aaacgagtga aaacgggcgt
    29641 catcaaggac gcagcgaatc acccgaccga tacgggctct gcccaggtcc agatcgctat
    29701 cctgagccgc aaaatcgatg agctcaccgc tcatctgaag aagaataaga aggatttcca
    29761 ctcgcgccgc ggcctcctgc agatggtcgc cgatcgccgg agccatcttc ggtacctcga
    29821 gaagagcaac aagcgcggat acaacgcctt ggtgaagaag ctcggcctca agaagtaagg
    29881 aaccgcgggg aacaccccgc gattcccgtt tccaagcagg tgctatcatt aatcgcgtgc
    29941 ggatcctcca tccgtactta ccaaatcttt taatacgcaa gctatgcccg tgatcaaaca
    30001 tccggcgcag cgagtcgggg ttttcataga tacgcagaac ctctatcaca gcgccaagca
    30061 tctttataaa tcgcgcgtga acttcgccaa cgtcctcgag gaggccgtcg ggaaccgcgt
    30121 cctcgtccgc gccatcgcct acgtcatctc caccgaaagc ggtgaggaga agaacttctt
    30181 cgaagcgctc acgaagatcg gtatcgagac caaggtgaag gggctccagg tgttctcaga
    30241 cggcgccaag aaggccgact gggacgtcgg gctcgccatc gatgccgtga agctcgcgcc
    30301 gaagctcgat accatcatcc tggcgaccgg cgacggcgac ttcgtcccgc tcatcgagta
    30361 cctcgatatg aacgagggct gccaggtaga ggtcatctcc ttcgggaagt cctcttccgc
    30421 gaagctgaag gaggccgcgc acgccttcac cgatatgtgc gagaacccga agcgcttcgt
    30481 gatcgcgcat cgagggtcct aacggcgcgc atccgcgcac gagcaggccg caggacgaac
    30541 atcctgtggc ccgttccggt tatactttga tgagcatgag cgattcgccg cagcagcccg
    30601 ccgaggataa atcgagattc atccgcacgt atgcgaagga tgtcgccgcg ctcacgggta
    30661 cctcggggaa ggcagcctcc gcgcctccgc cccctcccgt accgaagccc gtagcggctc
    30721 cgccaccacc cccacctaag ccgataccgc ctccacctcc gctggagaag ccggagtttg
    30781 aggcgcctgc gccccctccc gcgcccctgc cgcccgtgaa ccgcgcaccc gagcagccgt
    30841 ctcccatcca tacgtacaaa tcggatttcg ccgaccgcat cgaccagaag caggcctcga
    30901 ccttctcggt gcttgccgcg cagaaggacg cagggaagag accggcatca ccgaagaagc
    30961 gccgcagtca gctcatgctt gccggcgcgg tcgtcctcat cgcgctcggg ggcgcagggc
    31021 tcggcggcgc agcgtggtac gtcatgcgcg ccaacatgct tcccgcggcc gtgccgtcag
    31081 cgtcctcgct cgtcttcgcg gacgagcagg tgcgcctctc ggcctccggt cccgacctca
    31141 tgaacgagat cgcggcgctc gcgaacgaac ccctgcccga cggcagcgtg ctcgtggtgt
    31201 acctctcgga atccaccacg accgaacgtg gacgggtcat cgagaccccg ctttccggcg
    31261 gcgcgctcat caacgcgatg gagctcccgg cgccccagat cctgctccgc aacatctcgc
    31321 ccgagagcac ggtcggcatc atgagcgcag gaggggagac gcggcccttc ttcgtcttca
    31381 agaccagctc ctatgagcgc acgttcgcgg gtatgctcgc atgggagacg agcatcgcgc
    31441 gggacctcgc ggtgctcttc ccggccaagg ccgcaggcag cctcgccccg actgcggcga
    31501 gcgcgccgct tggcacctcg acccctgtcg cccagccgcc cgctcggccg ctcgacgctt
    31561 tcacggacgc ggtcgtcgcc aaccacgacg tgcgcgtgct ccgggacacc gccggagcga
    31621 ccctcatgct ctacgggtac gcccagaagg acctgctcat ccttgcgcgc gacgaagccg
    31681 cattcacgaa cctcatctcc cggctttcat caggggacta ggcagggtgt ggtagagtac
    31741 gctccatgaa tatcgaacac ttcaaggagc gcctctcgac cgagaaggcg acgctcgagg
    31801 ccgagctttc gaccgtgggg cgccgcaatc ccgccaaccc caacgactgg gaggcgatcc
    31861 cgcaggaggt ggggcaggag gccgatccga acgaccaggc ggacctcctc gagggcttcc
    31921 gggagaacac cgccatcctc acggacctcg agaaccgcta caacgatgtg gtcgccgcgc
    31981 tcgagcgcat cgaggcgggt acgtacggcg cctgcgcggc ctgcggtaac ccgatcgagg
    32041 aagatcggct cgaagcggag cccgcggcga agacctgcaa agcgcacctg aacggctagc
    32101 ccctcatgaa gctctccgtc aaggaatgcc tctcgttcgg atgggagacg ttcaagaagc
    32161 ggccgtggct ccttgcggga gcggtcgcgg tcggcgtgct cgtcgatggg ctcacctcgc
    32221 tcatcgagcg tgcgacccat ggttcataca tcctcgagcc gctcggcgtc ctgctcgcca
    32281 tcctcatttc catgctctac gcgaccgggc agacgaacct cttcgtgaag gcccatgacg
    32341 atccgatgtc cgcgcgcgtc gcggatcttc tgcacctccg cgcgtactgg cgcttcctgt
    32401 gcacctcgat cctgctctgg ctcttcatcg ggctgccgta cctggcgctc gagttccttt
    32461 cggtaccgcc cgtggtcatg gcgctcctca tcatcccggc gctcatcgtg tcgctcgtct
    32521 ttggctttgc gctctacctc gtcgtagacc gcggcatggg gcccctccag tccttgaagg
    32581 agagcgcgca gatcacgcgc gggcaccggc tcgagctctt cctgctcttg ctcgcgatcg
    32641 ttggggtgaa cctgctcggc cttgcggcgc tcgtcatcgg gctcttcgtc tccgtccccg
    32701 taaccgtgct cgccatggtg cacgcgtacc gcatgctctc ggggacgctc ccggtggcgg
    32761 ttgcgcccgc ggccggagcg gtctaggctt ggggtatccc atggccgcgc ttacgcccta
    32821 cccgtcctta accgactacc agcgcttcgc ggacgccgtc tacggcctga acaaccgtcg
    32881 gcacttcacc gccgacgaga tgctgaccaa tatcctgcgg ttcaccatgc ggggcctcaa
    32941 gggcatccgc aaaggggacc cggagaaggt gcggatgaat atgacgatcg ccatcagctg
    33001 gaccatgtcg ctcctgaccc agctcggcgt cgatctcgag cggcacgtct gggagcgctt
    33061 cccgtaccgc tgctcgtact gcgggcaatg cccctgcgcc tgcaaggcga agaaggtgca
    33121 gcggcgcgtg aaggtcgtgg tggacccccg aaggcgcccg cgcaccatcc gcgacctcca
    33181 ggtcatgttc aatgagatct atcccgcttc ctcccgcacc ctcgagcatg ccggcatcca
    33241 tctcgcggaa gagatcgggg agttctcaga ggccgtcatg acctaccgcg ggaaccataa
    33301 ggatgccgaa ttcgataagg tcatcctcga ggccgcggat ctctactcct gcttcatggg
    33361 tgtcttcaat tcgctcggca tcgattacga gaagggcctc gtcgcctcct tcaagcatgg
    33421 ctgccatgcc tgcaagcaca tcccctgcga gtgcgagtac cagtacgtgg tgaacttcaa
    33481 gtcctaaaaa ccccgctatg aggcggggct ttcggttagc gcgcgaacgg gttcttcgcg
    33541 ccgcggacct cgaagtggag gtggttgccg gtcgagcggc cggtgttgcc gacgtacccg
    33601 atgaggtcgc ccttggcgat ggcgccgcct gagaccgcgg tgcgggacat gtgcgcgtag
    33661 agggtctggg tgccgttgcc gtggctgatc acgatgtagt taccgtagcc gccgttatag
    33721 ccgtcgttgt tgcgggccac gacgaccgtg ccggctgcgg ctgcgtaaat aggggtgccg
    33781 gacggcgcgg cgatgtcgac gccgttgtag ccgtggacgc cctgggagag gcggccgccc
    33841 gggacggggt tgccgaagta gccggagacg gccgggaggt tccgggaggc cgctgccgcg
    33901 ccggagctct tcgtgcccgc ggtcttcgtg ctcttagcgg atgccttggt cggcagggag
    33961 ccgcccggca cgatgatgtc tgatcccgcc ttgaggtcag cggaggactc gaggccgttg
    34021 aagagggcca cttcctcggg gtccgcgccg tacttcttcg cgatcgcggc gatggtgtcg
    34081 cccgagcgca ccgtgtactg gaggcccgaa acagggagga tgaggagctc atcgccgggc
    34141 ttcacgagat tcgcgctctt aatgtcattg gcccacaaaa tggtgttcac cgtgaccccg
    34201 aagcgctcgg cgatctcgga gacgttgtcg ccctccttca cggtataggt cgtgatctgc
    34261 ccgccgccct cgcttgcggt ggcggccgcc tggcgcgggg agatggcgcc ctcggggccc
    34321 atcttcgcca ggagcgcggc gtcgccggac agcgcgaggt cgccgccgcc cttggcgggg
    34381 ttggggtcgc tattcaccgc agcatcgagc aattccatgc tcgaatcgtg ggtcagggta
    34441 tcggaaacct gcgcgcgggc acccgcgaac ggccagaagg cgcccgcaaa gcgggggagg
    34501 aggagcgcga ggaggctaag aatgaggcct gccgcaatca aggcggcggt cgcacgatat
    34561 gcatttccgt ttgcgacttt cgcggtggaa cgggaaggct gttgaggatt tgaaataagg
    34621 gaaacggggt agcttcagtc gttcttcaga atacaacaag gcttacatac ggggtatata
    34681 agccttactt gggtatcagc gattcactcg atgaagctag tataaagggt agggaggcag
    34741 ggtcaacctc aaccgttgca gggtgttgaa agccaatttt tggacgatga gtcaagtttt
    34801 ccgggcgtgg acagggactg cgcggtatat tgcaggaacg atgcagcact tagaggggtt
    34861 gaacgacgca cagaaggagg ccgtacttgc gaccgagggg ccgatctcgg tccttgcagg
    34921 cgccgggagc ggcaagaccc gggtcttaac tacccgcatc taccatttga tacgccaggg
    34981 ggtcgcgccg gagaagattc tggcggtcac attcacgaac aaggcggcgc gcgagatgcg
    35041 ggagcggctc gcagaacggc tcggggactc atcgctcccc atcgtcgtga ccttccacgg
    35101 gctcgggcgg atcatcctcg agcgcttcgg ctcgcggatc ggcgtcccac ggtatttcag
    35161 catcttcgac cgcgacgatt ccgagcgggc ggtgaaggag gccctgaagg cgctcgacgt
    35221 ggacccgaag gaagtggcgc cgcgtgccat cctcgggcgc atcagcaagg cgaaggggga
    35281 cgggatcacg gcccagcagt tcaaggagcg gtacggccgc gagagcttcc gcaaccggat
    35341 cgccgcagag gtctgggagc actacgacgc caccttgaag aaggagaagg cgctcgactt
    35401 cgatgacctc atctgtctgc cggtgcagct cctcgagggg catgaggacc tgcggggcga
    35461 agcggcggac ctcttctcgc atatccacgt ggacgag
//
LOCUS       DASPKG010000055        16086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_358185, whole genome shotgun sequence.
ACCESSION   DASPKG010000055 DASPKG010000000
VERSION     DASPKG010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16086)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16086
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358185"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..302
                     /locus_tag="VEA36_02260"
     tRNA            222..302
                     /locus_tag="VEA36_02260"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:255..257,aa:Leu,seq:caa)
     gene            308..1195
                     /locus_tag="VEA36_02265"
     CDS             308..1195
                     /locus_tag="VEA36_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HYF29167.1"
                     /translation="MKYLHLTLAITLALVVGAVPTYAATSTVEDATVNLYCRLTVGKK
                     IFGTSGSGVFVSDRGIILTNAHVAQYFLMPGDKSRVKGECHVRTGSPAESTYTAEVMY
                     FPSTWIDANLEALKQKVPKGSGEGDFALLRITGVKEGTMPERFPSLPLSFFGVAEGEL
                     VTVAGYPTDGRNFAELQNELDRIVTTTTVSNVRTFSLAGADLLTLASSTAAGAGVSGG
                     PVVRADGTLTGIVVAKSATTLRAITPLHINNRLVADTGVSLPGMFFGDLAARAAGNLL
                     LLDPKDLAALRQAFVKRNR"
     gene            complement(1266..1988)
                     /locus_tag="VEA36_02270"
     CDS             complement(1266..1988)
                     /locus_tag="VEA36_02270"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HYF29168.1"
                     /translation="MTEHAPFLWVGLDGLVRDRVETRAIAAELASVPGNFGFKVNLDY
                     ALAVGIGNLRRGPLKERPLFVDLKMLNGARTMTQILIALAHEGVTATNAYALAGGGES
                     YGGDELARSIAELRRQFSSTRMRVYGVTVLTHYGEAYSTAHFRAPLGKVVRMFAEECV
                     TAGADGVIMPGTQLKEVEHLRILKVLPGIRPSWYRDTRHEQETAPEAVAGRSDIEVVC
                     GSPIMKDRDPAAALTRILAALV"
     gene            complement(1990..2700)
                     /locus_tag="VEA36_02275"
     CDS             complement(1990..2700)
                     /locus_tag="VEA36_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29169.1"
                     /translation="MSLNPNTRALVHEMLDCGVLALRDVERGEEPFLYSSGNKGPGYA
                     MVKGLVAQRRLLRALVRELAIAIHEAAVGIEYVAGNATGGMVPAWILAEELTELSGKE
                     VPYFYVRGSRKVGGHSEQITGDDLNDFFTPGRRGIVMEELVNFAETTVNSALVQREAG
                     YEVNHGATILFYDHPVAAVRLASSSMELFHLITMKELLDEAEETGVFESRLIADYRRF
                     LADPPAWQRAHGLEPVVA"
     gene            complement(2823..3416)
                     /locus_tag="VEA36_02280"
     CDS             complement(2823..3416)
                     /locus_tag="VEA36_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007395849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYF29170.1"
                     /translation="MTSVRIGEQIPDYEFEAFVNDEAKTMKFSDFRGKWLVIMFYPAD
                     FTFVCPTELEEMAGQYPKIKEMNGEVISFSTDTVFVHKAWHDTSPAIKKIDFPMGADP
                     TGKIASAFGVLIEGGDLTFVVDEGLALRGTFIVDPNGILRTMEVHDNSVGRAAAETVR
                     KLRAAQFVEANPGMVCPAAWDEGDAGMEPGMDKVGKI"
     gene            3538..4374
                     /gene="miaA"
                     /locus_tag="VEA36_02285"
     CDS             3538..4374
                     /gene="miaA"
                     /locus_tag="VEA36_02285"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HYF29171.1"
                     /translation="MDTERRLKCVVGPTASGKSAHAIELAKQLDGEVISVDSRQVYRM
                     LDIGTEKITKEEMQGIPHHLIDIRDPKDPYSAGDFMKDADALISSLRERGKEPILAGG
                     THFYFDALLYGLPAETPANPLLRKQLDERSNQDLMREIRERDPRRAAKLDPSNKRRLV
                     RALEIIEQHGQVPEREHAVPRYQVEWIVLNPERDELRSRIHTRLQRALGNGLIEEVER
                     VRAYVGDERLNELGLEYKIVGEYLRGARDHSSLLPTLSSRIWHYARHQKAWLRKLTSD
                     RP"
     gene            complement(4355..5326)
                     /locus_tag="VEA36_02290"
     CDS             complement(4355..5326)
                     /locus_tag="VEA36_02290"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HYF29172.1"
                     /translation="MRTLLITLLITAALGLAAPAYAAESTFFGPIVPEECQSCPCGFA
                     GVLEIARHLMNFAVSFGIIVLTIMLVWGGILFIASAVNPESRSKAKSLLGGAFIGMAL
                     ILTSWLIVDFVMRALYSGPAGIEGKFGPWNSILAEDADWCIAAKEGKGFFEIPFMPLD
                     APNAEPTEADPNPNPTQVEPSPIVGNEKTVRQQLAAAGISVNKNPCPTGVRYQDVRGG
                     CTTVGGLRQNTVAQTITLKNICGSLIVTGGNELGHASGGMSHTTGYKVDYGTGIDACI
                     QGGSGGYFTRNGARGGDPRYLDKCGNEYVRESNPPHWDVTVVKVCPK"
     gene            complement(5328..5885)
                     /locus_tag="VEA36_02295"
     CDS             complement(5328..5885)
                     /locus_tag="VEA36_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29173.1"
                     /translation="MKRIAVIVGAILLVLILGVGVYLMTRGTDEAPQAPGAPPFPAGG
                     QLMPEPSSERLLIAAQGGEVEVNDFIHDGITVRDVVSPGRSVLVGDLGYCLADGSCPK
                     ATDVTRFHISYDEEGSFFTIVLLAEPLSEVRREAEQHLMNRLGIPEILACQLNYYVGT
                     PSFVNEAYSNGNMLFSFCEGATLLP"
     gene            complement(5882..6961)
                     /locus_tag="VEA36_02300"
     CDS             complement(5882..6961)
                     /locus_tag="VEA36_02300"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HYF29174.1"
                     /translation="MHRFRALLLLLLVAILIVPVPAIAQSSIPLLDPGWHIVPNPQEL
                     DSTCPEDAPLGIGGVLVLMQNLMNAAVALTVIVLILVIAYAGFLLVTSPFSSENRSKA
                     KSTLLNAVIGMALVLASWLIVDFVMKLIYEPESTFAGEALGPWNQILVGGDACIRGRD
                     GNPLFEIPFTRAPRPDTSVGLDRPGGTAGPGQPASLGAGACSPGTIATAAANGGYALT
                     AAQVQTLSCLAKAESSCGQNISGARSASGRTSTAHGMFQVVMGYDDKCHNLNIPVCSE
                     AAKRAGWKGSGNLNCSRAFSGGKVRPGMEELARVCRAASTNLDCNASAAACLVKENPN
                     FSDWTGTGDGHSHAAQKACVRQFNI"
     gene            7040..7113
                     /locus_tag="VEA36_02305"
     tRNA            7040..7113
                     /locus_tag="VEA36_02305"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7072..7074,aa:Trp,seq:cca)
     gene            complement(7152..7307)
                     /gene="rpmG"
                     /locus_tag="VEA36_02310"
     CDS             complement(7152..7307)
                     /gene="rpmG"
                     /locus_tag="VEA36_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HYF29175.1"
                     /translation="MSQDRLIKLRSTKSDHVYWSRKNKKKVERKIEVKKYDPTLRERV
                     VYKEVKK"
     gene            complement(7340..10015)
                     /locus_tag="VEA36_02315"
     CDS             complement(7340..10015)
                     /locus_tag="VEA36_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYF29176.1"
                     /translation="MPPFKNFTTKAKEAIKKAHELAIERGQNHVSPLHLLTALIHQEE
                     SLVFSVLDRLDIDTLLLADTLLEALEMPETSSTLSPSYNLYLTPDLAQAIEAAGKVAA
                     RMNDSFIGTEHLFVAVLEHPGQAGDILSRFRIDAETVTAVLKELKSAKDGQVAEPKRF
                     RALAKYTRNLTKLAADNALDPVIGRDLEINRVIQILSRRTKNNPVLIGEAGVGKTAIA
                     EGLAARIASGDVPESLKGKELLSLDLGLMVAGTKYRGEFEERMKNIMKEVEKSNGKVI
                     LFIDELHTIIGAGSAEGALDAGNMLKPALARGEIRVIGATTLKEYQQHIEKDAAFTRR
                     FQSVFVNEPSIEDAVAILRGLRDKYELFHGVRITDGAIVSAVDLSARYITDRYLPDKA
                     IDLIDEAASGLRIALENKPPLLEETDRKIRRLEIEREALKKDEESENAKEIKERVKAI
                     DAEIADLREKTGELELKWKNEKEVLQGIRAIKEELESLRVQADNAEAMADLGTAAEIR
                     YGRIPALQKELEAKTKRLKTLQKSRRILHEEVTEHEIAGVVSRWTGIPVAKMLEEEAA
                     KLARMEEALKDAVIGQDAAVKAIADAVKRSRVGIADPNRPIGSFLFLGPTGVGKTELS
                     RRLAEFMFNDPEALVRVDMSEFMEKHSVSKLIGAPPGYVGYEEAGSLTERIRHRPYAV
                     VLFDEIEKAHPEVFNILLQVLDSGHLTDGKGRKVNFKNTIVVLTSNIGAEFMDRMAGI
                     GFAQSGSDDAHQYQQVKDKVMEALKNQFRPEFLNRLDDIIIFDVLGKEALAQIVDKQV
                     QEVVGRLGGKRITLTLDPEVKAWLAEVGYNPQYGARPLRRAIQDKILTPIASLMVEQG
                     IMEGGSVQVTLKQGQPHFEVHKTARRKRSRAKAIA"
     gene            complement(10048..10824)
                     /locus_tag="VEA36_02320"
     CDS             complement(10048..10824)
                     /locus_tag="VEA36_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29177.1"
                     /translation="MDIEDLNKTQLLLLTLLVNFVTSIATGVLTVSLLDETSPTVTQT
                     VNRIVERTVESVTEAPATIPAIIPPKEVAAAPSDEERRTAAIAAAAARSVEIYKNASG
                     KVFVSRGTYLPKSKAVVTAETVFLPSEVAILFPDGTMVEASKSKSGGGLAIYGFADTA
                     VLPPAPGSNLVATADIKSGQTAIGLAADRSAVTGMVTKVEAAGISASVPGIPAGASIV
                     NLAGNVIGISQGTGVFIPAAAVNELLSAPTAGADDERAAS"
     gene            complement(10839..11978)
                     /locus_tag="VEA36_02325"
     CDS             complement(10839..11978)
                     /locus_tag="VEA36_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYF29178.1"
                     /translation="MVFGTTRIYLDWAAAAPVSPRALLAFSRAIALAGNPSSPHAEGR
                     AARDVLEDARVRIARLAGTKPDAVIFTSGATEANALGITGHLRALIEEGGDPAQLHVL
                     YLPSAHASVIGAMRALERIGVATEAITKLEAQLRPETVLVVVDLVCGETGQIYKVRDI
                     RRALDRTGRKIALFADASQAPRVESFELQRLGADMVSLDAQKVGGVRGIGALIAPRNI
                     PLRPLSLGGGQERGLRPGTESPALAAAFATALEECARDRSAFTAAARDRRIKLTEALR
                     AIPRMEINEGKLQAPHILNLSLIGRDTDYLLALLDEAGFAISTKSACETDEAGSRAVL
                     AMTDDAARASSTLRISWGPATKPRDLDRFADALISTVRFADQNAI"
     gene            12061..13539
                     /locus_tag="VEA36_02330"
     CDS             12061..13539
                     /locus_tag="VEA36_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HYF29179.1"
                     /translation="MARETRPPIVAVMGHIDHGKSSLLDYIRKANVVAGEAGGITQHV
                     SAYEAEHEHEGTTRTITFLDTPGHEAFRAIRSRGVATADVAILVVAADEGVKPQTIEA
                     FEAITEANVPYVVAFTKMDKNGADVERAKGSVLEHGIYLEGLGGSVPYAPVSSKSGDG
                     IPELLDLVLLTADLAELTYDPTLPASGFVLESSQDPKRGLSATLILKEGVLATGAYVV
                     AGSATAPVRFIDNFAGKRIESARAAQPVRISGWSELPSAGTPFATALNKKEAEKLAAG
                     YESSATNVRDLSAPEGMTVLPLIVKADVTGSIEAIKHELAKIAHDRVAIRIISEGIGA
                     VSEGDVKTAMAAAGTIVAFNVGTDSTARDLAERSSISIETFSIIYDLKERIEELVKER
                     APRITVEEVVGEAKVLKAFNTAGNKQVLGARWLSGTLGIGDTVKIERRGIEVGKAKLV
                     NLQVARMDVKDIHVEGEFGLQLEGRYEAAPGDTLQAIKMVEK"
     gene            13541..13867
                     /locus_tag="VEA36_02335"
     CDS             13541..13867
                     /locus_tag="VEA36_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HYF29180.1"
                     /translation="MSAERDQRALSILVEAAGRYISREAGRNTLITPTRATLAKDGKN
                     ATIYVSVFPEGQDEHAIKFLMRHKDLFRNELKKTTRLARLPYIRFEIDAGERARQHLD
                     DISKDL"
     gene            13888..13959
                     /locus_tag="VEA36_02340"
     tRNA            13888..13959
                     /locus_tag="VEA36_02340"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13920..13922,aa:Cys,seq:gca)
     gene            13972..14055
                     /locus_tag="VEA36_02345"
     tRNA            13972..14055
                     /locus_tag="VEA36_02345"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14006..14008,aa:Leu,seq:taa)
     gene            14057..15214
                     /gene="alr"
                     /locus_tag="VEA36_02350"
     CDS             14057..15214
                     /gene="alr"
                     /locus_tag="VEA36_02350"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HYF29181.1"
                     /translation="MEFIDTLRNIKRRFSPHRPLVEVGISKANLLHNLRTYQARYQNV
                     RIAPVLKSNAYGHDLGLIARLLDKEDIAFFMVDSFYEARKLRAAGVKGRIVVMGYVRP
                     TDPERSRLRDVDFALTDIEQLREISALLTRENRFHLKLDTGMHRQGILPADLPAAIEL
                     IKKNPNIKLVGIASHFADADGEDDAFSQAQVETWNEGVAEVERSFATIEYRHIAATKG
                     IRFGEATRTNVARVGIGLYGFDTSPEGTTDVRPVLELRSLITSIREIPAGDHVGYNIT
                     YTAKRPSRIATVPVGYYEGCDRRLSNKGAMLVQGAAAALIGRVSMNMSSIDVTDIPAA
                     KVGDPVIVISRDPSAPNSIDAIVERISTPEYRESEYVMLVHIPPHLRRVVE"
     gene            15277..15349
                     /locus_tag="VEA36_02355"
     tRNA            15277..15349
                     /locus_tag="VEA36_02355"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15310..15312,aa:Lys,seq:ctt)
     gene            15373..15457
                     /locus_tag="VEA36_02360"
     tRNA            15373..15457
                     /locus_tag="VEA36_02360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15407..15409,aa:Leu,seq:tag)
     gene            15560..>16086
                     /locus_tag="VEA36_02365"
     CDS             15560..>16086
                     /locus_tag="VEA36_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29182.1"
                     /translation="MSSIRINTVVSNVGSGFVALLLGSLVGVIMSLLVLEFSGLSSDW
                     WFLGFFIPMMLIARLLFSPQPELPVGNNGILKVFGERWTWVKFSEGKHGLLLGMSIEE
                     TSVQLKTTKPREVSALSKDNVKMMGLYFMTYYVRDMEKFQSSDEPLRSLEQKGDSKFR
                     DGIGGSDSETLEEPET"
BASE COUNT         2791 a         5131 c         5238 g         2926 t
ORIGIN      
        1 cggcaagcga tttgagcgcc gcctcatcaa actcctttct cggctggtac gggttcggct
       61 cgatgcgatc caccgccacc cagtacacgg aatcgtactt ggtgggctcg gtatatcccg
      121 ccggtttctt ctcgtcgtct cccccgaaga aattcatgcg cgtattgtag cattcggtcg
      181 cgctttgcgg agcggaatgt gtgcagtagg atggccatat cgccggggtg gtggaacggt
      241 atacacgggg gtctcaaaat cccctgccgc aaggcttgag agttcgagtc tctcccccgg
      301 cactcatatg aaatatctgc accttaccct cgccatcacg ctcgcgcttg ttgtcggcgc
      361 ggtcccgacc tacgcggcaa cctcaaccgt cgaggacgcg accgtgaacc tgtactgccg
      421 cctcaccgtc gggaagaaga tcttcgggac ctccggcagc ggcgtcttcg tgagcgatcg
      481 cggcatcatc ctcacgaacg cgcacgtggc ccagtacttc ctcatgccgg gcgacaagag
      541 ccgcgtgaag ggcgagtgcc atgtgcgcac cggctcgccc gcagaaagca cgtacaccgc
      601 cgaggtcatg tacttcccgt cgacctggat tgatgcgaac ctcgaggccc tcaagcagaa
      661 ggtgccgaag ggaagcggcg aaggggactt cgcgctgctg cgcattacgg gggtcaagga
      721 agggacgatg cctgaacgct tcccttcgct cccgctctcg ttctttggag tcgccgaagg
      781 ggagctggtg acggtcgccg gctaccccac cgacggtcgg aacttcgcgg agctccagaa
      841 cgagctcgac cggatcgtga ccacgacgac cgtttcaaac gtccgcacct tctcgctcgc
      901 cggcgccgac ctactgaccc ttgcctcctc gaccgcagcc ggcgccggcg tctcgggcgg
      961 ccctgtcgtg cgcgcggacg gcacgctgac cggcatcgtg gtcgccaaga gcgcgaccac
     1021 gctgcgcgcc atcaccccgc tgcatatcaa taaccggctc gtggcggaca ccggcgtctc
     1081 gctcccaggt atgttcttcg gagacctcgc agcgcgggca gcagggaatc tgctcctcct
     1141 tgacccgaag gatctcgccg cgctccggca ggcgttcgtg aagcgcaatc gataaactct
     1201 gcagataaag aggaagcccg cgccggagac cggcgtgggc ttgaattggt tgggcacccg
     1261 tcccgctaga cgagagcggc gagtatccga gtgagcgctg cggctgggtc acggtccttc
     1321 atgatggggc tgccgcagac gacctcgata tcgcttcttc cggcgaccgc ttcaggcgcc
     1381 gtttcctgct cgtgccgggt atcccggtac cagctgggcc ggatgccggg cagcaccttg
     1441 aggatgcgga gatgctccac ttccttgagc tgcgtgcctg gcatgatcac cccatcagcg
     1501 cccgccgtga cgcattcctc ggcgaacatc cggacgacct tcccgagcgg tgcccggaag
     1561 tgcgccgtgc tgtacgcctc gccatagtgc gtcaggacgg tgacgccgta gacgcgcatc
     1621 cgagtggacg agaactgcct gcgcagctcc gcgatcgatc gggcgagctc gtcaccgccg
     1681 taggattcgc cgccgcccgc aagcgcatag gcgttggtag cggtgacgcc ctcatgcgcc
     1741 agagcgatga ggatttgcgt catggtgcgc gccccgttca acatcttgag atcgacgaag
     1801 agcggacgtt ccttgagcgg accccggcgc aggttcccga tgccgaccgc aagggcgtaa
     1861 tcgagattga ccttgaagcc gaagttcccc gggacgctgg cgagctcggc cgcgatcgca
     1921 cgtgtctcca cgcggtcgcg tacgagcccg tccagcccga cccagaggaa gggtgcatgc
     1981 tccgtcatgt caggccacca ccggctcgag gccgtgcgcg cgctgccacg ccggcgggtc
     2041 ggcgaggaag cggcggtagt ccgcgatgag acgggactcg aacacgcccg tctcctctgc
     2101 ctcatcgagg agctctttca tcgtgatgag atggaagagc tccatcgagg aggaggcgag
     2161 ccgtaccgcc gcgaccgggt ggtcgtagaa gaggatggtc gcgccatggt tgacctcgta
     2221 gccggcctcg cgttggacca gcgcgctgtt gacggtggtc tcggcgaagt tcaccagctc
     2281 ctccatcacg atgccccgac gcccgggcgt gaagaagtcg ttcaggtcgt cgccggtgat
     2341 ctgctcgctg tggccaccca ccttgcgcga accgcggacg tagaagtagg ggacttcctt
     2401 accgctgagt tccgtgagct cttcggcgag gatccacgcg ggcaccatgc cgccggtcgc
     2461 attgccggca acgtactcga tgccgaccgc agcctcgtgg atcgcgatgg cgagctcccg
     2521 gacaagggct cgcaggagcc tgcgttgcgc gacgaggccc ttgaccatgg cgtagccggg
     2581 acccttgttc cccgacgagt acaggaacgg ctcttcgccg cgctccacgt cgcggagcgc
     2641 gagcacgccg cagtcgagca tttcgtgcac gagggcacgg gtattcggat tcagtgacat
     2701 gagcggcctc ccaggcctag tgaaagggac ggacggcgag ggccggtcca gtcgaaccct
     2761 agcacgtact caaataagcg aaaacagcgg tgcttcgacc gccgttccgg gagctatatg
     2821 tgttagatct tccccacctt atccatcccc ggctccatcc ccgcatcccc ctcgtcccag
     2881 gccgccgggc acaccatgcc cggattggcc tcgacgaact gcgcggccct cagtttccgg
     2941 accgtctccg ccgctgcgcg gcccaccgag ttatcgtgca cctccatggt gcgcagtatc
     3001 ccgttcgggt ccacgatgaa ggtgccccgg agcgcgagcc cttcgtcaac aacgaacgtg
     3061 aggtcgccgc cctcgatgag gaccccgaac gcgctcgcga tcttgccggt cgggtcggca
     3121 cccatgggga agtcgatctt cttgatcgca ggcgatgtgt cgtgccaggc cttgtgcacg
     3181 aagacggtat cggtcgagaa ggagatgacc tccccgttca tctccttgat cttcgggtac
     3241 tgcccggcca tctcctcgag ctcggtgggg cagacgaagg tgaagtcggc cgggtagaac
     3301 atgatcacga gccacttccc ccggaagtcg gagaacttca tcgtcttggc ctcgtcattg
     3361 acgaaggcct cgaactcgta gtccgggatc tgctcgccga tccgtaccga ggtcatatac
     3421 tcgccgtact gatcgtggaa gtccccgcaa tactcgcaat agttgctttt ctgttccttg
     3481 gccatacgga tatctcaatt aggtcccggt atgatagcat ttcgccaatt ctcgaccatg
     3541 gacactgaac ggaggctgaa gtgcgtcgtg ggcccgacgg catcggggaa gtcggcccac
     3601 gcgatcgagc tcgccaagca gctcgacggg gaagtcatct ctgtcgattc ccggcaggtg
     3661 taccgcatgc tcgatatcgg cacggagaag atcacgaagg aggagatgca gggcatcccc
     3721 caccacctca tcgacatccg ggaccccaag gacccgtaca gcgccggcga cttcatgaag
     3781 gacgcggatg cgctcatctc gtctctccgc gaacgcggca aggagcccat cctcgcgggc
     3841 ggcacccatt tctatttcga tgcgctcctc tacggattgc ccgcggaaac gcccgccaat
     3901 cccctgctcc gcaagcagct tgacgaacgc tcgaaccagg atctgatgcg ggagatccgc
     3961 gagcgcgacc cgcgccgcgc cgcgaagctc gacccgagca acaagcgccg tttggtacgc
     4021 gcgctcgaga tcatcgagca gcatgggcag gtgcccgagc gcgagcacgc cgtcccccgc
     4081 taccaggtcg aatggatcgt gctcaacccg gagcgcgatg agctgcgctc gcgcatccat
     4141 acccggctgc agcgggcgct cggaaacggg ctcatcgagg aagtcgagcg ggtgcgtgcg
     4201 tacgtcggcg acgagcgcct caacgagctc ggcctcgaat acaaaattgt cggggagtac
     4261 ctccgcggcg cgcgcgacca ctcctccctc ctccccactc tctcatcccg tatctggcac
     4321 tacgcgcggc atcaaaaggc gtggcttcgg aagctcactt cggacagacc ttaacgaccg
     4381 taacgtccca gtgcggcggg ttcgattccc gcacatactc gttcccgcac ttgtcgaggt
     4441 atcggggatc tcccccgcgc gcgccattgc gggtgaagta accgcctgag ccgccctgga
     4501 tgcaggcgtc gatgccggtg ccgtaatcga ccttgtaccc ggtcgtatgg ctcatgccgc
     4561 cgcttgcgtg cccgagctcg ttgccgcccg tgacgatgag cgagccgcag atgttcttga
     4621 gcgtgatcgt ctgcgcgacc gtgttctggc gcagcccgcc gaccgtcgtg cagccgccgc
     4681 ggacgtcctg ataccggacg ccggtcgggc atgggttctt gttcacggag atgccggcgg
     4741 ccgcaagctg ctggcgcacc gtcttctcgt taccgacgat gggcgacggc tcgacctgcg
     4801 tcgggttcgg gttggggtcc gcctcggtcg gctctgcgtt cggcgcgtcg agcggcatga
     4861 acggtatctc gaagaatccc ttcccttcct tcgcagcgat gcaccagtct gcgtcttccg
     4921 cgaggatgct gttccaggga ccgaacttcc cttcgatgcc cgcaggtccg ctatagaggg
     4981 cgcgcatgac gaagtccacg atgagccagg aggtcaagat gagcgccatg ccgatgaagg
     5041 cgcccccgag gagcgatttg gccttgctgc ggctctcagg attcactgcc gaggcgatga
     5101 acaggatgcc gccccagacg agcatgatgg tgagcacgat gatcccgaag gagaccgcga
     5161 agttcatgag gtgccgcgcg atctcgagca cgcccgcgaa cccgcagggg cagctctggc
     5221 actcctccgg cacgatcggg ccgaagaagg tggactcggc cgcgtacgca ggcgcggcaa
     5281 gaccaagcgc cgcggtgatg aggagcgtga tgagcagggt gcgcatatta cggaagcagg
     5341 gtcgcgccct cgcagaagct gaagagcata ttgccgttgc tgtaggcctc gttcacgaag
     5401 gagggcgtcc cgacgtagta gttgagctgg caggcgagga tctccgggat gccgagccgg
     5461 ttcatgagat gctgctccgc ctcgcggcgc acctcggaaa ggggttcggc aaggagcacg
     5521 atcgtgaaga agctcccctc ctcgtcgtac gagatgtgga agcgcgtgac gtcggtcgcc
     5581 ttcgggcagg agccgtccgc gaggcagtac ccgaggtcgc ccacaagcac ggaccggccg
     5641 gggctgacca cgtcccggac cgtgatgccg tcgtggatga aatcgttcac ctcgacctcc
     5701 ccgccctgcg cggcgatgag gagccgttcg ctcgatggct ccggcatgag ctgcccgcct
     5761 gccgggaacg gcggggcgcc cggcgcttgc ggcgcttcgt ccgtcccgcg cgtcatgagg
     5821 tagacgccga cgcccaggat gaggacgagg aggatcgcac cgacaatgac cgctatccgc
     5881 ttcatatatt gaattgtcgc acacaggcct tctgcgccgc gtgcgaatgt ccgtccccgg
     5941 tgccggtcca atcgctgaaa ttcgggttct ccttcacgag gcaggcggcg gcggacgcgt
     6001 tgcaatcgag gttggtggac gcggcacggc agacccgcgc gagctcctcc ataccggggc
     6061 gcaccttgcc gccgctgaac gcccgcgagc agttgaggtt gccgctgccc ttccatccgg
     6121 cgcgcttcgc cgcctcgctg cagaccggga tgttgaggtt atggcatttg tcgtcgtatc
     6181 ccatcacgac ctggaacatg ccgtgcgcgg tcgaggtccg gccgcttgcg gagcgcgccc
     6241 cgctgatgtt ctgcccgcag gagctttccg ccttcgcgag gcaggagagc gtctgcacct
     6301 gggcagcggt cagggcgtat ccgccgtttg cggccgcggt cgcgatggtg cctggcgagc
     6361 aggcgcccgc gccaagagac gccggctgcc cgggccctgc ggttccgccc ggccggtcga
     6421 gccccacgct cgtatcgggc cggggcgcac gcgtgaaggg gatctcgaag agcggattgc
     6481 cgtcccgccc acggatgcag gcatcgcccc cgacgaggat ctggttccag gggccgaggg
     6541 cctccccggc aaacgtggat tcgggctcgt agatgagctt cattacgaag tcgacgatga
     6601 gccaggacgc gagcacgagc gccatgccga tcacggcgtt caggagggtc gacttcgcct
     6661 tgctccggtt ctcactgctg aagggcgagg tgacgagcag gaagcccgcg tacgcgatga
     6721 cgaggatgag cacaatgacc gtgagcgcca ccgccgcatt catgaggttc tgcatgagga
     6781 cgagcacccc gccaatgcca agcggcgcgt cttcggggca ggtcgagtcg agctcctgcg
     6841 gattcggcac gatgtgccag ccgggatcca gcagcgggat gctggactgc gcgatggccg
     6901 gaaccgggac aatgagtatc gcgacaagaa gcaggaggag gagtgcccgg aaccggtgca
     6961 tggatgtatt gtagcttgct acggctgggc gaagcgcgcc tttgcccaca taccggtttc
     7021 tgctatcgtg ggcagtgtag ggcctatagt ttaatggtag aacgtcggtc tccaaaaccg
     7081 tcggtcgagg ttcgattcct cgtgggcccg ccagagcagg aagagaaaac cgcccctccg
     7141 ggcggcttct cttatttctt gacttccttg tacaccacgc gctcgcgaag ggtcgggtca
     7201 tacttcttga cctcgatctt ccgttcgact ttcttcttgt tcttgcggct ccagtagacg
     7261 tggtcggact tcgtggagcg aagcttgatg aggcgatcct gcgacatatg tagacccgac
     7321 tgtaccagag gcggcccggt taggcaatcg ccttggcccg cgagcgcttg cgacgcgccg
     7381 tcttatgcac ctcgaagtgc ggctggccct gcttcagggt gacctggacg gagccccctt
     7441 ccatgatccc ctgctccacc atcaaggagg cgatcggggt gaggatcttg tcctggatcg
     7501 cgcggcgcag cggccgggcg ccgtactgcg ggttgtatcc cacctcggcg agccacgctt
     7561 tcacctcggg gtcgagcgta agcgtgatgc gctttccgcc gaggcggccc accacctcct
     7621 ggacctgctt gtcgacgatc tgcgcgagcg cctccttccc gagcacgtcg aagatgatga
     7681 tgtcgtcgag gcggttgagg aactcggggc ggaactggtt cttcaaggcc tccatgacct
     7741 tgtccttcac ctgctggtac tggtgcgcat cgtcgctccc ggactgcgcg aagccgatgc
     7801 cggccatgcg gtccatgaac tcggcgccga tattcgaggt cagcactacg atggtgttct
     7861 tgaaattcac cttccggccc ttcccgtcgg tgaggtgccc ggaatcgagc acctggagca
     7921 ggatgttgaa gacctcgggg tgcgccttct cgatctcgtc gaagaggacg acggcgtacg
     7981 ggcggtggcg gatgcgctcg gtgagcgatc cggcctcctc gtacccgacg tatcccgggg
     8041 gcgcgccgat caacttcgag accgaatgct tctccatgaa ctccgacatg tcgacgcgca
     8101 ccagggcctc cggatcgttg aacatgaact cggcgagccg tcgggagagc tcggtcttgc
     8161 cgaccccggt ggggccaagg aagaggaatg agccgatggg gcggttgggg tccgcgatcc
     8221 cgacccgcga gcgcttcacg gcgtccgcga tggccttcac cgccgcgtcc tgcccgatga
     8281 ccgcgtcctt caaggcctcc tccatgcgcg cgagcttggc ggcctcctcc tcgagcatct
     8341 tcgcgaccgg gatgccggtc cagcgggaga cgacccccgc gatctcgtgc tcggtcacct
     8401 cttcgtgcaa aatgcgccgt gatttctgca gcgtcttcag gcgcttggtc ttcgcctcga
     8461 gctccttctg caacgccggg atgcggccgt accggatctc ggctgcggtc ccgaggtccg
     8521 ccatcgcctc cgcgttgtcc gcctgcaccc ggaggctctc gagctcctcc ttgatggcgc
     8581 ggatcccttg gaggacctcc ttctcgttct tccacttgag ctcgagctcg cccgtctttt
     8641 cgcgcaggtc cgcgatctcc gcatcgatgg ccttcacccg ctccttgatc tccttggcgt
     8701 tctcgctctc ctcgtccttc ttgagggcct cccgctcgat ctcgagtcgg cggatcttgc
     8761 ggtccgtctc ctcaaggagc ggcggcttgt tctcaagcgc gatgcgcagc cccgaggcgg
     8821 cctcgtcgat gaggtcgatg gccttgtccg ggaggtagcg gtccgtgatg tagcgggcgg
     8881 agaggtcgac cgcggagacg atcgcgccgt cggtgatgcg gaccccgtgg aagagctcgt
     8941 acttgtcccg gagcccgcgc aggatggcga ccgcgtcctc gatggagggc tcgttcacga
     9001 acaccgactg gaagcggcgc gtgaaggccg cgtccttctc gatgtgctgc tggtattcct
     9061 tgagggtcgt cgcgccgatg acgcggatct cgccgcgggc gagcgcgggc ttgagcatgt
     9121 tccctgcgtc gagcgcccct tccgcggagc ccgcgccgat gatggtgtgg agctcgtcga
     9181 tgaagaggat gacctttccg ttgctcttct cgacttcttt catgatgttc ttcatgcgct
     9241 cctcgaactc gccgcggtac ttcgtccccg cgaccatgag gccgagatcg agggagagga
     9301 gctccttccc cttgagggac tcggggacgt cgccggaggc gatgcgtgcc gcgagcccct
     9361 cggcgatcgc ggtcttgccg acgcccgcct cgccgatgag cacggggttg ttcttggtgc
     9421 ggcgcgagag gatctggatc acgcggttga tctcgaggtc ccgcccgatg accggatcga
     9481 gcgcgttatc ggccgcgagc ttcgtgaggt tgcgcgtgta cttcgcgagc gcgcggaagc
     9541 gcttgggctc cgcgacctgg ccgtctttcg cggacttgag ctccttcagg accgcggtca
     9601 cggtctccgc atcgatgcgg aagcgcgaga ggatgtcgcc ggcctggccg gggtgctcga
     9661 ggacggcgac gaagaggtgc tcggtgccga tgaacgaatc gttcatccgt gccgccacct
     9721 ttcctgccgc ctcgatggcc tgggcgaggt ccggggtgag gtagaggtta taggaaggag
     9781 aaagcgtcga gctcgtctcg ggcatctcga gcgcctcaag cagggtatcg gcgagcagga
     9841 gcgtatcgat atccagccgg tcgaggaccg agaacacgag gctctcctcc tggtggatga
     9901 gggctgttaa taggtggagc ggcgagacgt ggttctggcc ccgctcgatg gcgagttcgt
     9961 gggccttctt gatggcctcc ttggccttgg tagtgaagtt cttgaagggg ggcatatggc
    10021 tacgatgacg gtgccgccat gaaggaatta cgaagccgcg cgctcgtcgt ctgcgcctgc
    10081 ggtgggcgca gacaggagct cgttgacggc tgcggccggg atgaagacgc cggtcccctg
    10141 cgagatgccg atgacgttgc ccgcaaggtt cacgatcgag gcgccggcgg gaatgccggg
    10201 gacgctcgca gaaatgccgg ccgcctcgac cttggtcacc atgccggtga ctgcgctccg
    10261 gtcagcggca aggccgatcg cggtctgccc cgatttgatg tccgcggtcg cgacgaggtt
    10321 cgacccgggc gccggcggca gcacggcggt atcggcgaag ccgtagatcg cgaggccgcc
    10381 gccagacttc gacttcgatg cctcgaccat ggtgccgtcc gggaagagga tggcgacctc
    10441 gctcgggagg aacactgtct cggcggtgac gaccgccttg gatttcggga ggtaggtgcc
    10501 gcgcgagacg aagaccttgc cgcttgcgtt cttatatatc tcgacgctgc gcgcagcggc
    10561 ggcggcgatg gcggccgtgc ggcgctcctc gtcggagggc gctgccgcga cctccttcgg
    10621 cgggatgatg gccgggattg tggcaggcgc ctcggtgacg gactccacgg tgcgctccac
    10681 gatgcggttc acggtctggg tgaccgtcgg cgaggtctcg tcgagcaagg acacggtcag
    10741 cacccccgtc gcgatcgagg tgacgaagtt cacgaggagc gtgaggagga gcaattgggt
    10801 cttgttgagg tcttcgatat ccatagcgta tgtacctact atatcgcgtt ctggtccgca
    10861 aatcgaacgg tggaaataag cgcgtccgcg aagcggtcga ggtcccgcgg cttcgtcgcg
    10921 gggccccacg agatgcgcag tgtcgaggag gcgcgcgcgg cgtcatcggt catggcgagc
    10981 accgcgcgcg aacccgcctc gtccgtctcg caggcgctct tggtcgagat cgcgaatccg
    11041 gcttcgtcga ggagcgcaag gaggtaatcg gtatcgcgac cgatgagcga gagattgagg
    11101 atgtgcgggg cctggagctt cccttcgttg atttccatgc gagggatagc gcgtaacgcc
    11161 tcggtgagtt tgatccgtct gtcgcgagcg gctgcggtga atgcagacct atcccgcgcg
    11221 cattcctcaa gcgcggtcgc gaatgccgcg gcgagggccg gggactccgt acccggacgc
    11281 agtccccgct cctgcccgcc gcctagactg agcggtcgca ggggaatatt cctcggcgcg
    11341 atgagcgcgc cgatcccgcg caccccaccc accttctgcg cgtcgagcga gaccatgtcc
    11401 gcgccgaggc gctggagctc gaaggactcg acgcgcggcg cctgcgacgc gtccgcgaag
    11461 agcgcgatct tccggccggt ccgatcgagt gcgcggcgga tatcacgcac tttatagatc
    11521 tgtcccgtct cgccgcagac gaggtcgacc acgaccagta cggtctcggg acgcagctgg
    11581 gcttcaagct tcgtgatcgc ctcggtcgcg acgccgatgc gctcgagcgc tcgcatcgca
    11641 ccgatcacgg acgcatgcgc gcttgggaga tagaggacgt gcagctgcgc gggatcgcct
    11701 ccctcttcga tgagcgcgcg gaggtgcccc gtaatgccaa gcgcgttcgc ctcggtcgcg
    11761 ccgctggtga agatgaccgc atcgggcttc gtgcccgcga gccgcgcgat gcgcacccgt
    11821 gcatcctcga gcacgtctcg tgcggctcgt ccctccgcgt gcggcgagga tggattgccc
    11881 gcgagcgcga tcgctcgact gaacgcgagc agcgcacggg gcgagaccgg ggcggctgcg
    11941 gcccagtcga ggtagattcg cgtcgtaccg aataccatgt aggtacacta ggctaccggc
    12001 gttgcgcgcg caacggccga acattgagga aaagcgtatt ttatgacata atcgcccatt
    12061 atggcgcgcg agacccgacc acccatcgtg gccgtgatgg gccacatcga tcacggcaaa
    12121 tcatcgcttc tggattacat ccggaaggcg aacgtcgtgg cgggcgaagc gggcggcatc
    12181 acgcagcacg tctccgccta tgaggccgag catgagcatg aggggacgac gcgcacgatt
    12241 accttcctcg acaccccggg gcacgaggcc ttccgcgcta tccgctcgcg gggcgtcgca
    12301 acggcggacg tcgccattct cgtggtcgcg gccgacgagg gcgtgaagcc gcagaccatc
    12361 gaggcgttcg aggccatcac cgaagcgaac gtgccctatg tggtcgcgtt cacgaagatg
    12421 gataagaacg gcgcggacgt cgagcgcgcc aagggctcgg tcctcgagca cggcatctac
    12481 ctcgaggggc tcggcggctc ggttccctat gcaccggttt cctcgaagtc cggggacggc
    12541 atccccgagc tcctcgatct cgtccttctg accgccgacc tcgccgagct cacctatgat
    12601 ccaacgcttc ctgcgtcggg cttcgtgctc gagtcctcgc aggaccccaa gcgcgggctc
    12661 tccgccacgc tcatcctcaa ggaaggggtg cttgcgacgg gcgcgtacgt ggtcgcgggc
    12721 tccgcaacgg cgccggtgcg gttcatcgat aacttcgcgg gcaagcgcat tgagtccgca
    12781 cgggccgcgc agccggtgcg catctccggg tggtccgagc tcccgtccgc cggcacgccc
    12841 tttgcgaccg ccctgaacaa gaaggaagcg gagaagctcg ccgcgggcta tgagtcgagc
    12901 gccacgaacg tgcgcgacct ctcggcgccc gagggcatga ccgtgttgcc gctcatcgtg
    12961 aaggcggacg tgacaggttc catcgaggcg atcaagcacg agctcgcgaa gatcgcgcat
    13021 gatcgggtgg cgatccgcat catctcggag gggatcggcg cggtctcgga gggcgacgtg
    13081 aagaccgcga tggcggctgc tggcaccatc gtggcattca acgtggggac cgattcgacc
    13141 gcccgcgatc ttgcggagcg aagttccatt tccatagaga cgttctccat catctacgac
    13201 ctcaaggagc gcatcgagga gctcgtgaag gagagggcgc cgcgcatcac ggtcgaggag
    13261 gtggtcggcg aagcgaaggt gctcaaggcg ttcaatacgg ccggcaataa gcaggtgctc
    13321 ggcgcacgtt ggctctcggg cacgcttggg atcggggata cggtgaagat cgagcggcgc
    13381 ggcatcgagg tcggcaaggc gaagctcgtc aacctgcagg tcgcccgcat ggacgtgaag
    13441 gacatccacg tcgagggcga gttcgggctc cagctcgagg gcaggtacga ggcggcgccg
    13501 ggcgatacgc tgcaggccat caagatggtc gagaaataat atgtcggccg agcgggacca
    13561 gcgggcgctc tccatcctcg tcgaggcggc cgggcgctac atctcgcgcg aggcgggacg
    13621 caacaccctc atcaccccga cgcgcgccac gctcgccaag gacggcaaga acgccaccat
    13681 ctatgtctct gtcttccccg agggccagga cgagcatgcg atcaagtttc tcatgcggca
    13741 taaggatctc ttccgcaacg agctcaagaa gaccacgcgg ctcgcccgcc ttccctacat
    13801 ccgcttcgag atcgatgcgg gcgaacgcgc gcgccagcac ctcgacgata tttccaaaga
    13861 cctgtagccg cgctatcatc cccgtacggc caggtggcga agtggtaacg ctgcggattg
    13921 caaacccgct atgcacgggt tcgattcccg tcctggcctc aagcgaaaaa cgccggggtg
    13981 atgaaattgg tagccataga ggtcttaaac acctcaggca gcaatgccgt gtgggttcga
    14041 gtcccacccc cggtacatgg aattcatcga tacgctcagg aacatcaaac ggcgattttc
    14101 gccgcaccgc cccttagtcg aggtcggcat ctcgaaggcg aacctcctgc ataacctgcg
    14161 cacgtaccag gcacggtacc agaatgtccg catagcgccg gtgctcaaat cgaacgcgta
    14221 tgggcacgac ctcggcctca ttgcccgcct cctcgacaag gaagacattg ccttcttcat
    14281 ggtcgactcg ttctacgagg cgaggaagct ccgcgccgcg ggggtgaagg gccgcatcgt
    14341 cgtgatggga tacgtgcgtc ccacggatcc cgagcgctcg cggctccgag acgtggactt
    14401 cgcgctcacg gatatcgagc agctccggga gatctccgcg ctcctcaccc gcgagaaccg
    14461 cttccacctg aagctcgaca ccggcatgca ccgccaaggc atcctcccag ctgacctccc
    14521 tgccgccatc gagctcatca agaagaatcc gaacatcaag ctggtgggga tcgcctcgca
    14581 ctttgcggac gcggacggcg aggacgacgc attcagccag gcgcaggtcg agacctggaa
    14641 cgaaggggtc gcggaagtgg agcgctcctt cgcgacgatc gagtaccgcc acatcgccgc
    14701 gaccaagggc atccgcttcg gcgaagcgac ccgcacgaac gtggcgcggg tcgggatcgg
    14761 gctctatggc ttcgacacct ccccagaggg cacgaccgat gtgcgccccg tgctcgagct
    14821 gcgctcgctc atcacctcga tccgcgagat ccccgcgggc gaccacgtgg ggtacaacat
    14881 tacctatacg gcgaagcgcc cttcgcgcat cgcgacagtc ccggtcggct actacgaggg
    14941 ctgcgaccga aggctctcca acaagggagc gatgctcgtc cagggcgcgg ccgctgcgct
    15001 catcggccgg gtatcaatga atatgagttc gatcgatgtc acggatatcc ctgcggcgaa
    15061 ggttggagac ccggtgatcg tgatcagccg cgacccgagc gcgccgaatt ccatcgatgc
    15121 gatcgtcgag cggatcagca cgcccgagta ccgggaatcc gaatacgtga tgctcgtgca
    15181 catcccgccg cacctgcgcc gcgtggtgga ataggggtgg cgaatatagg agtttccgct
    15241 atactccgaa ttaaccgcat tgcgcggttt tcatttgcgc ccatagctca gttggtagag
    15301 cactcgcctc ttaagcgaag ggtcccaggt tcgagtcctg gtgggcgcac cgacgtagta
    15361 ggatggcgat atgccggggt ggcgaaattg gtagacgcac cacgcttagg acgtggctcc
    15421 ctcgggaatg gaggttcgag tcctctcccc ggcaccaata cagggttttc tggggcttga
    15481 ctttttgtat aaaatggtgc tatcatattg actggtccga agggaccttg aaaactaggc
    15541 aacattcaag gagagccaga tgagctctat caggatcaac acagtggtgt cgaatgtagg
    15601 aagcgggttt gtcgctctcc tgctcggcag cctggtgggg gtgatcatga gcttgctggt
    15661 tctcgaattc agcgggctct catccgattg gtggtttctc ggcttcttca tcccgatgat
    15721 gctcatcgct cggctgctct tcagccctca gcctgagctt cctgttggga acaacgggat
    15781 cctcaaggtg tttggcgagc gctggacctg ggtcaagttc tcagagggca aacacgggct
    15841 cctgctcggt atgagcatcg aggagacctc ggtgcagctc aagaccacca aaccgcggga
    15901 ggtctctgcc ctatcgaagg ataacgtcaa gatgatgggg ctctacttca tgacctacta
    15961 cgtccgcgat atggagaagt tccagagctc agatgagccc cttcgttcgc tagaacaaaa
    16021 gggcgacagc aagttcaggg acggcatcgg cggcagcgat tcagaaaccc ttgaggagcc
    16081 tgaaac
//
LOCUS       DASPKG010000056        10007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1712212, whole genome shotgun sequence.
ACCESSION   DASPKG010000056 DASPKG010000000
VERSION     DASPKG010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10007)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10007
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712212"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VEA36_02370"
     CDS             complement(<1..331)
                     /locus_tag="VEA36_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HYF29183.1"
                     /translation="MHLLGDRVFASIVALLVIGGAGIFLSAALGLLARESVSLGHIAV
                     TQLLLGLIPGVIALLILRFMPPAWLQKLVVPLYLFSILLTCLVFVPGLGVHAGGATRW
                     IDLGPFTV"
     gene            455..733
                     /locus_tag="VEA36_02375"
     CDS             455..733
                     /locus_tag="VEA36_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29184.1"
                     /translation="MPISIPGWLFFFAAFIVCMAFDMKKLIANPLVGATRIETLVVIG
                     FVFLLVGQGCKAYGWTLAATITLGFAMAIVPLGLLLRLLLAIGSRRLR"
     gene            complement(796..1854)
                     /locus_tag="VEA36_02380"
     CDS             complement(796..1854)
                     /locus_tag="VEA36_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29185.1"
                     /translation="MILDAARVIVPFVAAFVIGIGIAPLITHYLYKHRAWKKKGGKVG
                     MGDGHGTPLFNELHKEREVSTPRMGGIVVWASVFITALLIMLMGVFLGEPFSSFAFVN
                     REQTWLPLFALMVGALVGLADDILEVTRSSGGLSLRWRLLVVAAIGAFSGWWFFAKLG
                     VSTVGIPFLGALPLGSMFIVFFVVVTLALYAGGIIDGIDGLAGGIFAIIFMVYAAIAF
                     AQQQYSLAAFAATVAGALFAFLWFNIPPARFYLSETGTMALTVALAVTAFSADSLGEG
                     VGVAVLPIIALPLTVTVVTNMMQIVSKKVFKRKLFRIAPIHHHFEAIGWPAYKVTMRY
                     WVITIIAGVFGLALALLK"
     gene            complement(1851..2801)
                     /locus_tag="VEA36_02385"
     CDS             complement(1851..2801)
                     /locus_tag="VEA36_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYF29186.1"
                     /translation="MTDKKAALRSLITQYEARQRRQMGIAILAGTTVLALAVFLAPKP
                     APEPVEVAVEEGAPAVDAYEDLALSGKAAIVYDLATGEVLFERNAKAQLPLASLTKLL
                     TVYAAATSMPRDARVTLTDAALAADGESGFAAGETFTLDDLAKLALVSSSNDATEAIA
                     LATESHRAQSGPTLLASAAAAIGLSQTYALNGTGLDESTSVSGGYGSATDVAKLAGAL
                     LGAAPEIARASTEPALTIHSTTGVAHTLSNTNPDVVRVPGIMLSKTGFTDLAGGNLVV
                     VYDAGIGHPVAVVVMGSTREGRFTDVAKLIKRTADHFAGL"
     gene            complement(2798..3337)
                     /locus_tag="VEA36_02390"
     CDS             complement(2798..3337)
                     /locus_tag="VEA36_02390"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HYF29187.1"
                     /translation="MAPEARLLAILFAAGEALPKKRVASLLSISADELRDASTKLGEQ
                     LSGLALVETEEELELRTAPEAANDVKALREGELSRDLGKASLETMAMILYRGGATRGE
                     IDFVRGVNSAAAIRNLLLRGLIERIDDPADRRRAKYLPTVEALAHLGVKKREDLPRFA
                     EFTATIATEEARATADETV"
     gene            3427..3840
                     /locus_tag="VEA36_02395"
     CDS             3427..3840
                     /locus_tag="VEA36_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HYF29188.1"
                     /translation="MALKTLPTKKSVPAFLKGSPRPEEGAALAKLFAGATGEKPVLWG
                     DSIVGYGRYHYKSERSRQEGDWPLTGFSPRKGALTVYVMPGFKPYGALLKKLGPHTTS
                     VGCLYIKRLDAIHLPTLKKLIKQSVKDMRKKYGAA"
     gene            3837..4607
                     /locus_tag="VEA36_02400"
     CDS             3837..4607
                     /locus_tag="VEA36_02400"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HYF29189.1"
                     /translation="MTTLDTMILGAIEGFTEFLPISSTGHLILAAELLGLPSTEFLKS
                     FQIAVQLGAIAAVLLLYFRTFLDIELLKRLFVAFLPTGLVGFLVYPYVKEFLLGNSLV
                     VVISLFVGGVALIVFELLHKERWEHEIDLRDITYKQAAFVGIFQSIAIIPGVSRSAAT
                     IVGGLLAGLPRVSIVEFSFLLAVPTMVAATGYDLLKSAHQFTKSDAELMAIGFIVSML
                     VAFASMKFLLGIVRRYSFIPFGIYRIVIAVLFFVVVIL"
     gene            complement(4602..5207)
                     /locus_tag="VEA36_02405"
     CDS             complement(4602..5207)
                     /locus_tag="VEA36_02405"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYF29190.1"
                     /translation="MDKQQLVIIHGGNARNSYEEYLDRLKQREVTADDFKVKHKWKEV
                     FADEFSSSWEVFKPSMPNDKNAKYLEWKIWFEKLVPYLRDDVVFIGHSLGGLFLAKYL
                     SEEQFPKRVRATILVAAPYNTPEHPRADFNITHPFDQLVKQGGEIYLYHSPDDETVPL
                     SAFERYAKEIPSAHAVLIENRGHFSTPTFPELAEVVRSLKL"
     gene            complement(5200..5934)
                     /locus_tag="VEA36_02410"
     CDS             complement(5200..5934)
                     /locus_tag="VEA36_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HYF29191.1"
                     /translation="MEGLATGFHIKTEAYEGPFELILDLIEKRQLSINELSLSQVTDD
                     YIQHVRGHGTFPMEDAAQFIGVAATLLLIKSKSLIPDLELTGEEEEDMDDLKRRLREY
                     EKVREMMRELSKIFGKRVMVAAGERAPEPVFSPARDLTIKKLEEALADALAALEKEEQ
                     LPEARVRPTVTIEEMMESLLTRIQKKMTMSFREFAAGSTEKVEVIVSFLALLELVKEG
                     AVGAEQQGSFGEIQITNASAGVPHYG"
     gene            complement(5922..7328)
                     /locus_tag="VEA36_02415"
     CDS             complement(5922..7328)
                     /locus_tag="VEA36_02415"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HYF29192.1"
                     /translation="MKLTLNKPDINRTPKDYTRIAFVAEKGVRRLVRENGVQTIELSV
                     PEKLNLRKFVILCRAVVAAAKQYKARKIALDFNQTPELWKGLADVPAGRIAQAAAENF
                     EMANFEFRTYKTKPKEGWTEIEEIMVCGPQTKETLAGAKKGQIIGQGVNACRELANTP
                     GGDITPRKLAAAAKAAAKGLPITVTTLGRAQMEKLGMGALLGVAKGSSEEPTFTIMEY
                     KGAAGAPIVLAGKGITFDSGGLNLKPSNSIYEMHMDMSGAAAVIQAVAIAARLKLKRR
                     VIGLVPSAENMPGNNAYRPGDVLKSLSGKTIEVLNTDAEGRLILADAITYAKKFKPAV
                     VIDAATLTGAALTALGLYASGLLTCDDKLAQTLSELGETSGDYVWRLPLWDEYEEALK
                     GQFADLSNIGTGQTGRYGGAIEGGMFLWQFAKELDCPWAHLDIAPRMTAAPGDELAKG
                     AAGAPVRLLVSFIESWKG"
     gene            complement(7309..8685)
                     /gene="aspS"
                     /locus_tag="VEA36_02420"
     CDS             complement(7309..8685)
                     /gene="aspS"
                     /locus_tag="VEA36_02420"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010887698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HYF29193.1"
                     /translation="MAERTLIADLSGKAGESVLIQGSVEVRRDQGKLVFFDFRDRSGT
                     VQGVVLPGSEAMETAKETRTEFVVSVEGVVNRRPEKNIVAGKLNGDIELEVQGMQVLA
                     QADAMPFEFEGELNLDTYLDHLPLTLRGKRARAIFKVQAEILKAYRAFLDSEGFTEFQ
                     APKLIGDDAEGSGEVFEVPYFYGKTAHLATSPQLYKQIMVGVYERVYATGIVFRAEKH
                     STSRHLNEYTSLDAEMGFIESHLDVMRVENRLMRHIAEHLKQAAPAEFELLGAEIPEV
                     PAEIPRLKLREAQKLIHDNIGETFGEEVLTENILEMPDLDPAHERWLCKWAKREHGSD
                     FIFITHYPVAKRPMYTMEDPEDPGFTNSFDLLFRGVEITTGGQRRHEYENLVDGIRMK
                     GLDPEQFSFYLQAFKYGMPPHGGWGMGLERLTAKFLNLQNVKEATLFPRDINRIDTLL
                     SKHEADPE"
     gene            complement(8721..9674)
                     /gene="trpS"
                     /locus_tag="VEA36_02425"
     CDS             complement(8721..9674)
                     /gene="trpS"
                     /locus_tag="VEA36_02425"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HYF29194.1"
                     /translation="MNERPILLSGMKPTGIAHIGNYFGALKQWVDLQEAGEHDCYFMI
                     ADYHALNFIQDAGQMRELSRELARTLLAIGLDPGRVTIFKQSGVPAHTELAWVFDTIT
                     TMPYLMRAHAFKDAEAKDKEINVGTFNYPMLMAADILLYDTAVVPVGQDQKQHIEYAR
                     DTAEKFNRTYGEVFRLPKEHILPEVAVVPGTDGQKMSKSYGNTIPLFASREEIQKAVM
                     GIVTDSSGARPENVYAIHKLVRSEAELAPIYEEHQGKYQALKEALIEDLEAFIAPMRE
                     RYEALGADEGRLDDVLAAGGQKARERAEAKMAEVRKAIGVA"
     gene            complement(9747..>10007)
                     /locus_tag="VEA36_02430"
     CDS             complement(9747..>10007)
                     /locus_tag="VEA36_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HYF29195.1"
                     /translation="ETRIRYLDDHSKKKRGDEETFNLGGQDESDSDSVVRTYSCNSPL
                     GRAIVGKEVGEIVEVLTPDKETYEIEILAYAIPAEDEEAQAA"
BASE COUNT         1766 a         3282 c         2999 g         1960 t
ORIGIN      
        1 gtaccgtgaa ggggccgaga tcgatccagc gggtcgcccc gcccgcatgc accccgagcc
       61 ccggcacgaa gacgaggcag gtcaggagta tagaaaagag atagagcggt accacgagct
      121 tctggagcca tgcgggcggc ataaagcgca ggatgaggag cgcgatgacg cccgggatca
      181 gcccgaggag cagctgcgtc accgcgatat gccccagcga aacgctctcg cgggcaagga
      241 gcccgagcgc tgcggagagg aagatgccgg cacccccaat caccaggagc gcgacaatag
      301 aggcgaagac ccggtcgccg agcagatgca tggattagtt atagcatcag ccgtaccagc
      361 tccagcgtat aaggatgcgt gaatattgac aaagcgctcc cttactatat attatatata
      421 accctacgaa cagggcgctc tttggagaca agcgatgccc atcagcattc ccgggtggct
      481 atttttcttc gccgcgttca tcgtctgcat ggccttcgac atgaagaagc tgatcgcgaa
      541 cccgctcgtc ggcgccacga ggatcgagac actcgtggtc atcggcttcg tgtttctgct
      601 ggtcggccag ggttgcaagg cctatggctg gaccttggcc gcgaccatca cgctggggtt
      661 cgccatggcg atcgtaccgc tcggcctgct gctgcggctt ctgctcgcga tcggctcaag
      721 gcggctgcgc tagcccgcgc ctctcatcgg cccggtacaa acggtcgtgt ccactggttg
      781 ggtgcgaccg ttctcttatt ttagtagcgc cagcgcgagc ccaaatacgc ccgcaatgat
      841 cgtgatcacc cagtaccgca tggtgacctt gtatgccggc cacccgatcg cctcgaaatg
      901 gtgatggatg ggcgcgatgc ggaacagctt ccgcttgaag accttcttcg agacgatctg
      961 catcatgttc gtcaccaccg tgacggtgag cggcagcgcg atgatcggca gcaccgctac
     1021 cccgaccccc tcaccgagcg aatccgccga gaaggccgtc accgcaagcg cgaccgtcag
     1081 cgccatggtc ccggtctcgg acaagtagaa gcgcgcaggc gggatattga accagaggaa
     1141 ggcgaacagg gctccagcga cggttgccgc gaaggccgcg agcgagtact gctgctgcgc
     1201 gaacgcgatc gccgcataca ccatgaaaat gatcgcgaag atgccgccgg cgagcccatc
     1261 gatgccgtcg atgatcccgc cggcatagag cgctaaggtc acgaccacga agaagacgat
     1321 gaacatgctc ccgagcggca gcgccccaag gaacggtatg cccaccgtag acaccccgag
     1381 cttcgcgaag aaccaccagc cgctgaaggc gccgatcgcc gcgaccacca ggagccgcca
     1441 gcggagtgag agcccgcccg aggagcgggt cacctcgagg atgtcgtcgg caaggcccac
     1501 cagggcgccc accatgagcg cgaacaaggg cagccacgtc tgttccctat tcacgaacgc
     1561 gaaggacgag aagggctccc ccaggaagac gcccatcagc atgatgagga gcgcggtgat
     1621 gaacaccgac gcccagacca cgatcccgcc catccggggc gtcgagacct cccgctcctt
     1681 atgcagctcg ttgaacaagg gcgtcccgtg cccgtccccc atgcctacct tcccgccctt
     1741 cttcttccac gcccggtgct tatagaggta atgggtgatc aggggcgcga taccgatgcc
     1801 gatcacgaag gcagcgacga acggtacgat gacccgggcg gcatcgagga tcataggccg
     1861 gcgaagtgat cagcggtgcg cttgatgagc tttgccacgt ccgtgaaccg cccctcccgc
     1921 gtcgagccca tgaccacgac cgcgaccgga tgcccgatcc cggcgtcgta gaccacgacg
     1981 aggttcccgc ccgcaaggtc cgtgaagccc gtcttcgaga gcatgatgcc cgggacgcgt
     2041 acgacgtccg gattggtatt ggaaagcgta tgcgcgacgc ccgtcgtcga gtggatagtg
     2101 agcgcaggct ccgtacttgc ccgtgcgatc tcgggagcgg cgccaagcaa ggcgcccgcg
     2161 agcttcgcga catcggtcgc ggacccgtat ccgcccgaga ccgaagtgct ctcgtcgagc
     2221 ccggtgccat tcaatgcata ggtctgcgag agcccgatcg ccgctgcggc gctcgcgagg
     2281 agcgtcggcc ccgactgcgc ccgatggctc tcggtcgcaa gcgcgatcgc ctcggtcgcg
     2341 tcgttggagc tcgagacgag cgcgagcttc gcgaggtcat cgagcgtgaa ggtctcgccg
     2401 gctgcaaacc cgctctcgcc gtccgcggca agcgcggcgt ccgtcagggt gacgcgcgca
     2461 tcccgcggca tcgaggtcgc ggccgcatag accgtgagga gtttcgtcaa ggacgcgagc
     2521 ggcagctgcg ccttcgcgtt gcgctcgaac agaacctcgc cggtcgcaag gtcgtagacg
     2581 atggccgcct tccccgaaag cgcgaggtcc tcatacgcgt ccaccgcagg cgcaccctcc
     2641 tcgaccgcga cctcgaccgg ctcgggcgca ggctttgggg cgaggaacac cgcgagcgcg
     2701 agcaccgtgg tgcccgcgag gatcgcgatg cccatctgcc ggcgctggcg cgcttcgtac
     2761 tgggtgatca gggaccggag tgctgctttc ttgtccgtca taccgtttcg tctgcggttg
     2821 cgcgggcctc ttccgtcgcg atggtcgccg tgaactccgc gaaacgcgga agatcctccc
     2881 gctttttcac gcccaggtgc gcgagcgcct cgaccgtcgg caggtacttt gcccggcggc
     2941 ggtccgcagg atcatcgatg cgctcgataa ggccgcgaag caagagattc ctgatcgccg
     3001 cggcggaatt cacgccgcgc acgaagtcga tctcgccgcg cgtcgcgcct ccccggtaga
     3061 ggatcatcgc catcgtctcg aggctcgcct tgccgaggtc ccgtgaaagc tcgccctcgc
     3121 ggagcgcttt cacgtcgttt gcagcttccg gggcggtacg cagctcgagc tcctcctccg
     3181 tctcgacgag cgcaaggccc gagagctgct ccccgagctt cgtagaggca tcccggagct
     3241 catcggcaga gatcgagagc agcgatgcca cgcgcttctt cggaagcgcc tcacctgcgg
     3301 caaagagaat agcgagcaga cgggcttcgg gcgccatgat tcccttacaa tacccgcttc
     3361 aggactggac gacaaccctc attttgtgtg ctcgtgggga gtgtcgcgga tgctacactt
     3421 tccttcatgg cactgaagac cctccccacg aagaagagcg tgcctgcatt cctcaaaggc
     3481 tcgccccgtc ccgaagaggg cgcggcgctc gcgaagctgt ttgcgggggc gaccggcgag
     3541 aagcccgtcc tctggggcga ctccatcgtc gggtatgggc gctaccacta caagagcgaa
     3601 cgctcgcggc aggagggcga ctggcccctg accgggtttt caccccgcaa aggcgcgctc
     3661 accgtctacg tcatgccggg gttcaagccc tacggcgcgc tcctcaagaa gctcgggccg
     3721 catacgacga gcgtcggctg cctctacatc aagcgcctgg atgccatcca cctccccacc
     3781 ctcaagaaac tcatcaagca gtccgtgaag gatatgcgta agaagtacgg agccgcatga
     3841 ccaccctcga taccatgatc ctcggcgcga tcgagggctt caccgagttc ctccccatct
     3901 cctcgacggg ccacctcatc ctcgccgcag agctgcttgg cctcccgtcg accgagttcc
     3961 tcaagtcctt ccagatcgcg gtgcagctcg gggcgatcgc ggcggtcctc ctgctgtact
     4021 tccgtacctt cctcgacatc gagctcctca agaggctctt cgtggccttc ctccccaccg
     4081 gcctcgtcgg cttcctcgtc tatccgtacg tgaaggagtt cctcctcggc aattccctcg
     4141 tggtcgtcat ctccctcttc gtcggcggcg tagcgctcat cgtcttcgag ctcctccata
     4201 aggagaggtg ggagcatgag atcgatctca gggacatcac gtacaagcag gctgcgttcg
     4261 tcggcatctt ccagtccatc gccatcatcc ccggggtctc gcgctccgca gccaccatcg
     4321 tcggcggcct cctggccggc ctgccgcgcg tctccatcgt cgagttctcc ttcctccttg
     4381 cggtcccgac gatggtggcg gcgaccggct acgatctcct caagagcgcg caccaattca
     4441 cgaaatccga cgccgagctc atggcgatcg gcttcatcgt ctcgatgctc gtcgccttcg
     4501 cctcgatgaa gttcctgctc ggcatcgtgc gccggtacag cttcataccc ttcggcatct
     4561 accgcatcgt catcgcggtg ctgttcttcg tagtggtgat cctatagctt taagctccgc
     4621 actacctctg ctaattcggg gaatgtcggt gtactgaagt ggccgcggtt ctcgatcagg
     4681 acggcgtgcg cgctcggtat ctccttcgca taccgttcga aagccgagag cgggacggtt
     4741 tcatcatccg ggctgtggta gagatatatt tctcctccct gcttcaccaa ctgatcgaac
     4801 gggtgggtga tattgaaatc tgctcgtgga tgctcgggcg tattgtaggg tgccgcaacg
     4861 agtatggtcg ctcgcacccg cttagggaac tgctcctcag acagatactt agcgaggaag
     4921 aggccgccga gcgagtgtcc gatgaagacc acgtcgtccc ggagataggg cacgagcttc
     4981 tcgaaccaga tcttccactc gaggtatttc gcgttcttgt cgttgggcat gctgggcttg
     5041 aagacctccc aggaggaact aaactcgtcg gcaaacactt ctttccactt gtgctttacc
     5101 ttgaaatcat cggcagtgac ctcgcgctgc ttcagcctat cgaggtactc ctcgtacgaa
     5161 ttccgggcgt taccgccgtg aatgatcacc agctgttgct tatccatagt gcggcacgcc
     5221 cgccgacgcg ttcgtgatct ggatctcccc gaagctcccc tgctgctcgg cacccaccgc
     5281 cccctccttc acgagctcga gcaaggccag aaacgatacg atcacctcca ccttctccgt
     5341 gcttcccgca gcgaactccc tgaaggacat ggtcatcttc ttctggatgc gcgtgaggag
     5401 gctctccatc atctcctcga tggtcacggt cggccggacc cttgcctccg gcagctgttc
     5461 ctccttctcg agggcggcaa gcgcgtcggc gagcgcttcc tcgagcttct tgatcgttaa
     5521 atcgcgcgcg ggcgagaaca cgggctcggg cgcgcgttcc ccagccgcga ccatcacccg
     5581 cttcccgaag atctttgaga gctcccgcat catctcgcgg accttctcgt attccctgag
     5641 gcggcgcttc agatcgtcca tgtcctcttc ctcctcgccg gtcaattcga ggtccggaat
     5701 gagggacttt gacttgatga ggagcagggt cgcggcaacg ccgatgaact gcgccgcgtc
     5761 ctccatgggg aaggtgccgt gcccgcgcac gtgctggatg tagtcgtcgg tgacctgcga
     5821 aagcgagagc tcgttgatcg agagctgccg cttctcgatg aggtcgagga tgagctcgaa
     5881 ggggccctcg taggcctcgg tcttgatgtg aaagccggtg gctagccctt ccatgactct
     5941 atgaatgata ccaagagccg gacgggagcg ccggcagcgc ccttagcgag ctcgtcaccg
     6001 ggagcggccg tcattctcgg cgcgatgtcg aggtgcgccc aggggcaatc gagctccttc
     6061 gcgaactgcc agaggaacat gccgccctcg atcgcgccgc cgtagcgtcc cgtctgcccc
     6121 gtcccgatgt tcgagaggtc cgcgaactgt cccttgagcg cctcctcgta ttcgtcccag
     6181 agcgggaggc gccagacgta atcgccggag gtctcgccga gttcggagag ggtctgcgca
     6241 agcttatcgt cgcacgtcag gagcccgctc gcatagagcc cgagcgccgt aagcgctgcc
     6301 cccgtcaggg tcgcggcatc gatgaccacc gcgggcttga acttcttcgc gtacgtgatc
     6361 gcgtccgcga ggatgagccg cccctcggca tccgtattca gcacctcgat ggtcttcccg
     6421 gacagggact tcagcacgtc gcccggccta tacgcattgt tgcccggcat gttctccgca
     6481 gaaggcacga gcccaatgac gcgcctcttc agcttcaagc gcgcagcgat cgcaaccgcc
     6541 tggatgacgg ccgccgcgcc cgacatatcc atgtgcatct cgtagatgga gttcgagggc
     6601 ttcaggttga ggccgcccga atcgaacgtg atgcccttcc ccgcgagcac gatcggggcg
     6661 ccggcggcgc ccttgtactc catgatggta aaggtcggct cctcgctcga gcccttcgcg
     6721 accccaagca gcgcgcccat cccgagcttc tccatctgcg cgcgacccaa ggtcgtgacg
     6781 gtgatcggca ggcccttcgc cgcggccttc gcggctgccg cgagcttccg gggcgtgata
     6841 tccccgcccg gcgtattcgc gagctcgcgg caggcgttca ccccctgccc gatgatctgt
     6901 cccttcttcg cgcccgcgag cgtctccttg gtctgcggcc cgcataccat gatctcctcg
     6961 atctccgtcc agccttcctt cggcttggtc ttgtatgtcc taaactcgaa gttcgccatc
     7021 tcgaagttct cggcagcggc ctgcgcgatg cgtcccgcgg gcacgtccgc aagtcccttc
     7081 caaagttcgg gggtctggtt gaaatcgagc gcgatcttcc gagccttgta ttgcttcgcc
     7141 gccgcaacta cggcgcggca gaggatgacg aacttccgga gattgagctt ctccggcacc
     7201 gagagctcga tcgtctgcac gccgttctcg cgtacgaggc gtcgcacgcc cttctcggcc
     7261 acgaacgcga tccgcgtgta gtccttgggc gttctattga tatccggctt attcagggtc
     7321 agcttcatgt ttggaaagta acgtatcgat gcgattgata tcgcggggga agagggtcgc
     7381 ttccttcacg ttctgcagat tgaggaactt ggcggtcagt cgctcaaggc ccatacccca
     7441 gccgccatgc ggcggcattc cgtacttgaa ggcctggagg tagaaggaga actgctccgg
     7501 atcgagcccc ttcatgcgga tgccgtcgac gaggttctcg tactcatgac gacgctggcc
     7561 cccggtggtg atctcgacgc cccggaagag aaggtcgaag ctgttcgtga acccggggtc
     7621 ctcgggatcc tccatggtgt acatgggacg cttcgcgacc gggtaatgcg ttatgaatat
     7681 gaaatccgag ccgtgctcgc gcttcgccca cttacagagc catcgttcat gcgctgggtc
     7741 gagatcgggc atctcgagga tgttctcggt aagcacctcc tcgccaaagg tctccccgat
     7801 attgtcgtgt atgagcttct gggcttcacg gagcttcagg cgtgggatct ctgcaggtac
     7861 ctccgggatc tcggcgccga gcaactcgaa ctcggcggga gccgcctgct tcaagtgttc
     7921 agcgatatgc ctcatgagcc gattctcgac gcgcataaca tcgagatggc tctcgatgaa
     7981 gcccatttcc gcgtcgagcg aggtgtattc gttcaggtgt cgcgacgtgc tgtgcttctc
     8041 ggcgcggaat acgatgccgg ttgcgtacac ccgctcatag acgccgacca tgatctgctt
     8101 gtagagctgg ggcgacgtag cgaggtgcgc ggtcttcccg tagaaatacg ggacttcgaa
     8161 cacctctcca ctgccttctg cgtcgtcgcc gatcagctta ggggcctgga actcggtgaa
     8221 tccttcggaa tcgagaaagg cacggtaggc cttcaggatc tcggcctgga ctttaaagat
     8281 tgcgcgagca cgcttgccgc gcagcgtgag cgggagatga tcgagatacg tatcgaggtt
     8341 gagctcgccc tcgaattcaa agggcatcgc atcggcctgc gccagcacct gcatgccctg
     8401 cacctcaagc tcgatatccc cgttcaattt ccctgcaacg atattcttct cgggccgcct
     8461 gttcaccacg ccctcgaccg ataccacgaa ctcggtacgg gtctccttcg cggtctccat
     8521 cgcttccgag cccggcagca ccaccccctg gaccgtgccc gagcggtccc ggaaatcgaa
     8581 gaagacgagc ttgccctggt cccgacggac ctcgacagag ccctggatga ggacactctc
     8641 cccagccttg ccggaaagat cggcaatgag cgtgcgttca gccatatagc ctataaagta
     8701 gcacgtcccc tgctttttgc ttaggcgacg ccaatggcct tccggacctc cgccatcttc
     8761 gcctctgcac gctcgcgcgc cttctgcccc cctgcagcaa ggacgtcgtc gagccggccc
     8821 tcgtccgcgc cgagcgcctc gtaccgctcg cgcatgggcg cgatgaacgc ctcaaggtcc
     8881 tcgataagtg cctccttgag cgcctgatac ttcccctggt gctcctcata gatgggtgcg
     8941 agttccgctt cgctccgtac gagcttgtgg atcgcgtaca cgttctcagg gcgcgcgcct
     9001 gaggaatccg tcacgatgcc catgacggcc ttctggatct cctcccgtga ggcgaagagc
     9061 ggaatcgtgt tgccatagct cttgctcatc ttctgaccgt cggtcccggg caccaccgcc
     9121 acctcgggaa ggatgtgctc cttcggtaat cggaagacct ccccgtaggt gcgattgaac
     9181 ttctccgcgg tatcccgcgc gtactcgatg tgctgtttct ggtcctgacc gaccggcacg
     9241 actgccgtat cgtacaggag gatgtcggcg gccatgagca tcggatagtt gaaggtcccg
     9301 acgttgatct ccttatcctt cgcttccgcg tccttgaagg catgcgcccg catgaggtag
     9361 ggcatggtcg tgatggtatc gaagacccag gcgagctcgg tatgcgctgg cacccccgac
     9421 tgcttgaaga tggtgacgcg ccccgggtcg agcccgatcg caagcagggt ccgcgcgagc
     9481 tcccgggaca gttcccgcat ctgccccgca tcctggatga agttgagggc gtgatagtct
     9541 gcgatcatga aatagcagtc atgctctcct gcctcctgga ggtccaccca ctgcttcagg
     9601 gcgccgaaat agttgccgat gtgcgcgata ccagtgggct tcatgccgga gagtaggata
     9661 ggccgttcgt tcatgaagct acgataccac aaaagaaaaa gcgccgcggg actgcccgcg
     9721 gcgcgaaagg tggtcgtcgg tggtggtcag gcggcctgtg cctcctcgtc ctccgccgga
     9781 atggcgtagg cgaggatctc gatctcgtag gtctccttgt cgggagtcag gacttcgacg
     9841 atctcgccga cctccttgcc cacgatggct cggccgagcg gactgttgca gctgtaggtc
     9901 cggaccacgc tgtcgctgtc gctctcgtcc tggccgccga ggttgaaggt ctcctcgtcg
     9961 ccgcgcttct tcttgctgtg gtcgtcgagg tagcggatgc gggtctc
//
LOCUS       DASPKG010000057         5470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_495888, whole genome shotgun sequence.
ACCESSION   DASPKG010000057 DASPKG010000000
VERSION     DASPKG010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495888"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /gene="rpsH"
                     /locus_tag="VEA36_02435"
     CDS             complement(<1..308)
                     /gene="rpsH"
                     /locus_tag="VEA36_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HYF29196.1"
                     /translation="MVSDRVGDFIIRLKNAGAIGKTSVAVPYSKHVEEIAKKLQALGF
                     VKSVEASKNPYEFEVTLAYDERGRSKIRGVKRISKPGRRLYTPSTKAHNVKNGAGSRI
                     L"
     gene            complement(319..588)
                     /gene="rpsN"
                     /locus_tag="VEA36_02440"
     CDS             complement(319..588)
                     /gene="rpsN"
                     /locus_tag="VEA36_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HYF29197.1"
                     /translation="MAKTSQLARNAKRVKLAEKYAEKRAALKAAGDYEALQKLPRNSS
                     LVRVKNRCNISGRQRGFMRAFGVSRIVFRELARDGKIPGVKKASW"
     gene            complement(705..1586)
                     /locus_tag="VEA36_02445"
     CDS             complement(705..1586)
                     /locus_tag="VEA36_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29198.1"
                     /translation="MPDDTSLVPVHEEHILVDIARVAQETEGSDLKKAIGLDGDRTLF
                     PMAIEVFGELDAIIKGAMEDQRTPYRKKRKSSSPIYYITEFDETKPHRMFVFIPPGGT
                     ALYDAVRSYLRHYKGATVVLTLFQRIRRGDKKIGVGPRLLLNSCALMKHPTDWMVLDD
                     APPELMLSAGLSDEKRGRVFVRFRPPERSTRFFECVMQVYNKDRPEAEWATYHLSEWA
                     EKELVVTPLTDVDRIYFLGLNQAQGLREILGEVAPIADMASRPLRQAQPFPAPAQLLA
                     ARQTLRLLPPDKGEKKE"
     gene            complement(1712..2245)
                     /gene="rplE"
                     /locus_tag="VEA36_02450"
     CDS             complement(1712..2245)
                     /gene="rplE"
                     /locus_tag="VEA36_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HYF29199.1"
                     /translation="MIISKEKQASAFAALKEKFGYTNVFASPRLVKIVVSTGVGKADK
                     NRKAVVEDRLAKITGQKAAPRAAKQSIAQFKMRQGDIVGYQVTLRGARMNDFLDKLVH
                     IALPRTRDFRGLNPTAIDDMGNLTIGIKDNTIFPETSDEDLKDVFGLAVTLVSTAKNK
                     EEAEAFFRHLGLPLKAA"
     gene            complement(2256..2600)
                     /gene="rplX"
                     /locus_tag="VEA36_02455"
     CDS             complement(2256..2600)
                     /gene="rplX"
                     /locus_tag="VEA36_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HYF29200.1"
                     /translation="MSKASKGKQTAKFKIKKGDHVVVISGKDKGKTGAVLRVMPEDAT
                     VVVEGIGMVKRHIKKAGRGQSGRIVERPRAIHISNVMVEDPKEKKGTRVRRESKDGAR
                     TRVTVKSGTALS"
     gene            complement(2612..2989)
                     /gene="rplN"
                     /locus_tag="VEA36_02460"
     CDS             complement(2612..2989)
                     /gene="rplN"
                     /locus_tag="VEA36_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HYF29201.1"
                     /translation="MVQPQTIVTVADNSGAKVARVFKVLGGSKRRYAEIGDVVVVSVQ
                     TAEPRKAVKKKDIYKAVVVRQRKPFRRANGIYVRFDDNAVVLIEKQGKEMGPKGNRVF
                     GPVPRDLVERGWQNIASLAPEVV"
     gene            complement(2996..3238)
                     /gene="rpsQ"
                     /locus_tag="VEA36_02465"
     CDS             complement(2996..3238)
                     /gene="rpsQ"
                     /locus_tag="VEA36_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HYF29202.1"
                     /translation="MENNTTKPQTFRGTVVKTAMKDTATVAVSRYVKHPKYKKYQVRT
                     KKFLVDDPGNTVEVGQQVTIIATRPISKRKSFKIAK"
     gene            complement(3248..3439)
                     /gene="rpmC"
                     /locus_tag="VEA36_02470"
     CDS             complement(3248..3439)
                     /gene="rpmC"
                     /locus_tag="VEA36_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HYF29203.1"
                     /translation="MAKKTSFKDKDVQELRKLLAEKREELREARFAATGARVKDVNQA
                     AKIRKDIARLLTEITAKTA"
     gene            complement(3442..3861)
                     /gene="rplP"
                     /locus_tag="VEA36_02475"
     CDS             complement(3442..3861)
                     /gene="rplP"
                     /locus_tag="VEA36_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HYF29204.1"
                     /translation="MLFPKKVKHRKWQTGRKSPERLARPETRGITVAFGSYGLKATSA
                     SRVTSNQIEAARKVLTRVTGKGGKMWIRIFPDRPITAKPAEVGMGKGKGDPKHFVFEV
                     RPGRVLFELDGVDDAKARDALRRAGAKLPLKTTVVTR"
     gene            complement(3868..4560)
                     /gene="rpsC"
                     /locus_tag="VEA36_02480"
     CDS             complement(3868..4560)
                     /gene="rpsC"
                     /locus_tag="VEA36_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HYF29205.1"
                     /translation="MTHTVHPYAHRLGIIRDWKSRWFAGDKKSFRENLAVDAAIRAYL
                     KKKLRAMYISDIEIERNAAEVRIILNTGRPGLVIGRSGENATKLRAELTKLVKKHSPN
                     STRTVKLDIMEIRQPEADAAVVAYMVAEGLEKRMPFRRILKMTVEKVMAVREVLGVRI
                     VVSGRLGGADMSRTEQIKRGRIPLQTFRANISYAEERAYLPYGVIGIKVWIYLGETFN
                     EKDRAAAPRQAK"
     gene            complement(4568..4939)
                     /gene="rplV"
                     /locus_tag="VEA36_02485"
     CDS             complement(4568..4939)
                     /gene="rplV"
                     /locus_tag="VEA36_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HYF29206.1"
                     /translation="MASKGTQKNKVEKVVPLASASLATYNQSPRKVRLVTDLVKGKTV
                     AEALAALTFLPKRAALPIKKLIESAVANAKQKGEDVEGLRIKDIRVDSAGMLVRYMPR
                     AMGRAAPIRKRRSKVVLALGK"
     gene            complement(4943..5290)
                     /gene="rpsS"
                     /locus_tag="VEA36_02490"
     CDS             complement(4943..5290)
                     /gene="rpsS"
                     /locus_tag="VEA36_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HYF29207.1"
                     /translation="MSRSLKKGPFVDVKLMKKIQDKKPATAGVIKTWARRSQIAPEFV
                     GFTFGVHNGKQHVEVLVTDEMVGHRLGEFSPTKKFVRHGGKMQKQLEQKKQQTEIAAA
                     KAAKAAPAAPKKK"
BASE COUNT          911 a         1710 c         1675 g         1174 t
ORIGIN      
        1 aggatgcgcg agcctgcgcc gttcttcacg ttgtgcgcct tggtggacgg cgtgtagagg
       61 cggcggccgg gcttagagat gcgcttcacg ccacggatct tcgagcggcc gcgctcatcg
      121 tacgcgagcg tcacctcgaa ctcgtagggg ttcttcgacg cctcgacgct cttcacgaac
      181 ccgagcgcct ggagcttctt cgcgatctcc tcgacatgct ttgagtacgg cacggcaacg
      241 gaggtcttgc cgatcgcgcc ggcattctta agacggatga tgaaatctcc tacgcggtcg
      301 cttaccatac gaaaatgctt accaggaggc cttcttaacg cccgggatct tcccgtcccg
      361 cgccagttcg cggaacacga tgcgggagac gccgaacgcg cgcatgaagc cacgctggcg
      421 tccagagata ttgcagcgat tcttcacccg gaccagcgaa gagttcctcg ggagcttctg
      481 gagggcctcg tagtccccgg cggccttcaa ggcagcgcgc ttctctgcgt acttctcggc
      541 gagctttact cgcttggcgt ttcgcgccag ctgggaggtc ttggccataa gaaaaatagc
      601 tccgaggagc gtgcaagtaa tgtatcagtc aggaattagg ctgtcaaaca aagaaaaagt
      661 gccccgcccc cgaggagcgc ggcactcttt gaaggacagt cctcctactc cttcttctcg
      721 cccttatcgg gcgggaggag cctgagggtt tgccgtgcag cgagcaattg cgccggggcc
      781 ggaaacggct gcgcctgccg aagcgggcga gacgccatat cggcgatcgg agctacctcc
      841 ccaaggatct cgcgtaatcc ctgggcctgg ttcaggccca ggaagtaaat gcgatccaca
      901 tcggtgagcg gggtgacgac gagctccttt tcagcccact cgctgaggtg ataggttgcc
      961 cattccgcct ccggccggtc cttgttgtag acctgcatga cgcactcgaa gaaacgcgtg
     1021 ctgcgctctg gaggccggaa ccttacgaac acgcgaccgc gcttctcatc gctgaggccg
     1081 gcgctcaaca tgagctccgg cggcgcgtca tcgagcacca tccagtcggt cgggtgcttc
     1141 atgagcgcgc aggagttgag cagcagacgc ggcccaaccc ctatcttctt gtcgccccta
     1201 cgaatccgct ggaacagcgt cagcactacg gtcgcgccct tatagtggcg aagatagctc
     1261 cgaaccgcat cgtacagcgc cgttccgcca ggcggaatga agacgaacat gcggtgcggc
     1321 ttggtttcat cgaactcggt aatgtagtag atcggcgatg agctcttccg cttcttccga
     1381 taaggtgtcc gctgatcctc cattgccccc ttgatgatgg catcgagctc cccgaatacc
     1441 tcgatggcca tcggaaaaag agtgcgatca ccgtcaaggc cgatggcctt cttgagatcg
     1501 gaaccctccg tctcctgcgc tacccgggca atgtccacga ggatgtgctc ctcgtggacg
     1561 gggactaaac tggtgtcatc aggcatgtca tttctcccca cgtgaacaac tcgtcacggc
     1621 gtttggaatc cgggcaaact ctatcactcc tctataaaaa cgctagaaat atgaagaacg
     1681 gcccagcttg ggccgttctt catgcgttct gttacgccgc cttcagcgga agccctaagt
     1741 gccggaagaa tgcctcagcc tcttccttgt tcttcgcggt cgagaccaag gtcaccgcga
     1801 ggccgaacac gtccttgagg tcttcatcgg aggtctccgg gaagatcgta ttgtccttga
     1861 taccgatggt gaggttgccc atgtcgtcaa tcgccgtcgg attgaggccg cggaagtcgc
     1921 gcgtgcgcgg gagcgcgatg tgcacgagct tatcgaggaa gtcgttcatg cgcgcgccgc
     1981 ggagcgtgac ctggtagccc acgatgtcgc cctggcgcat cttgaactgc gcgatggact
     2041 gcttggccgc acgcggcgcc gccttctggc ccgtgatctt cgcgagccgg tcctcgacga
     2101 ccgccttgcg attcttgtcc gccttgccga cgccggtcga gaccacgatc ttcacgaggc
     2161 gcggggacgc gaagacgttc gtgtacccga acttctcctt gagtgccgcg aacgcgcttg
     2221 cctgcttttc ctttgagatg atcatacgta gatgcttagg agagcgcggt gccgctcttt
     2281 accgtaacgc gggtgcgcgc cccgtccttc gattcccggc gtacccgggt ccccttcttc
     2341 tccttggggt cctcgaccat gacgtttgag atatggatcg cgcgcgggcg ctcgacgatg
     2401 cgaccggact gcccgcgacc cgccttcttg atgtgccgct tgaccatgcc gatgccctcg
     2461 acgaccaccg tcgcgtcttc cggcatcacc cggagcacgg cgccggtctt gcccttgtcc
     2521 ttgcccgaga tcacgacgac atggtcgccc ttcttgatct tgaactttgc ggtctgcttg
     2581 cctttggatg ctttggacat atgcaaatgg gttagacgac ctcaggcgcc aaggacgcaa
     2641 tgttctgcca gccgcgctcg accagatcgc ggggcaccgg accgaacacg cggttgccct
     2701 tcgggcccat ctccttgccc tgcttctcga tgaggaccac ggcgttgtca tcaaagcgca
     2761 cgtagatgcc gttggcgcga cggaagggct tcctctggcg cacgaccacc gccttgtaga
     2821 tgtccttctt cttcaccgcc ttgcggggct cggcggtctg gaccgatacg acgacgacgt
     2881 cgccgatctc cgcatagcgg cgctttgagc cgccgagcac cttgaagacg cgcgcgacct
     2941 tggcgcctga gttatctgca acggtgacga ttgtctgggg ctggaccata aatgtttact
     3001 tggcgatctt gaaggacttg cgcttggaga tgggcctcgt cgcgatgatg gtgacctgtt
     3061 gcccgacctc gaccgtattg cccggatcgt ccacaaggaa cttcttcgtg cgcacctggt
     3121 acttcttgta cttcgggtgc ttcacgtagc gggagaccgc gacggtggcc gtgtccttca
     3181 tggccgtctt aacgacggtt ccgcggaagg tctggggctt ggtggtgttg ttttccatga
     3241 gagtaattta ggcggtcttg gccgtgattt ccgtgaggag gcgcgcgatg tccttgcgga
     3301 tcttcgcggc ctggttgacg tccttcacgc gggcgccggt cgcggcgaac cgcgcctcgc
     3361 gcagttcctc ccgcttctcg gcgaggagct tcctcagctc ctgcacgtcc ttatccttga
     3421 atgaggtctt cttggccata cttagcgggt gacgacggtc gtcttaagcg ggagcttggc
     3481 gccagcgcgc cgcagcgcat cgcgcgcctt tgcatcgtcg acgccgtcga gctcgaagag
     3541 gacgcggccg ggccggacct cgaacacgaa gtgcttgggg tcgcccttac ccttacccat
     3601 gcccacctcg gccggcttcg cggtgatggg acggtccggg aagatccgga tccacatctt
     3661 gccgcccttg ccggtgacgc gagtgagcac cttgcgggcc gcctcgatct ggttggaggt
     3721 cacgcgggac gcgctcgtcg ccttcaggcc ataggagccg aacgcgaccg tgatgccgcg
     3781 ggtctcggga cgcgcaagac gctccgggga cttgcgtccg gtctgccact tgcggtgctt
     3841 cactttcttc gggaacagca tacgaaatta tttagcctgg cgcggtgcgg ccgcgcggtc
     3901 cttctcgttg aaggtctcgc cgaggtagat ccagaccttg atgccgatca cgccgtacgg
     3961 gaggtacgcg cgctcctcgg cgtatgaaat gttcgcgcgg aaggtctgga gcgggatgcg
     4021 gccgcgcttg atctgctcgg tgcggctcat gtccgcgccg ccaaggcgcc ccgacacgac
     4081 gatacggaca cccaggactt cgcggaccgc catgaccttc tcgaccgtca tcttgaggat
     4141 gcggcggaac ggcatgcgct tctcgagccc ttccgcgacc atgtacgcca cgaccgcggc
     4201 atccgcctcc ggctggcgga tctccatgat gtcgagcttc accgtgcggg tggagttcgg
     4261 ggaatgcttc ttcacgagct tcgtgagctc ggcgcgcagt ttcgtcgcgt tctcgcccga
     4321 gcgcccgatc acgaggccgg gacgccccgt gttgaggatg atgcggacct cggcggcatt
     4381 gcgctcgatt tcaatatcag aaatgtacat ggcgcggagc ttcttcttga ggtatgcgcg
     4441 gatagcggca tccaccgcga ggttctcgcg gaagctcttc ttgtcgcccg cgaaccagcg
     4501 gctcttccag tcacggatga tgccgaggcg gtgcgcatag ggatgtacgg tgtgtgtcat
     4561 acgaaaatta tttgccgagc gcgaggacga ccttgctgcg gcgcttcctg atgggcgctg
     4621 cgcggcccat ggcgcgcggc atgtagcgca ccaacatgcc ggcggagtcg acccggatat
     4681 ccttgatgcg cagcccctcg acgtcctcgc ccttctgctt ggcgttcgcg accgccgact
     4741 cgatcagctt cttgatcggg agcgcggcgc gcttggggag gaaggtaagc gcggcaaggg
     4801 cctccgcaac ggtcttcccc ttcaccagat cggtgacgag gcgcaccttg cggggcgact
     4861 ggttgtaggt ggcaagcgat gcgctcgcga gcggcacgac cttctcaacc ttattcttct
     4921 gggttccctt gcttgccata ccttacttct tcttcggtgc ggcgggcgct gccttggcgg
     4981 ccttcgcagc ggcgatctcg gtctgctgct tcttctgctc gagctgcttc tgcatcttac
     5041 cgccgtggcg cacgaacttc ttggtgggcg agaactcgcc gaggcggtgc cccaccatct
     5101 cgtcggtcac gagaacctcc acgtgctgtt tgccgttatg caccccgaag gtgaagccga
     5161 cgaattcagg cgcaatctgg ctccggcgtg cccacgtctt aatcaccccc gcggtggcgg
     5221 gtttcttgtc ctggatcttc ttcatgagct tgacgtccac gaagggtcct ttcttgagcg
     5281 atcgcgacat gagtagttaa aagcccgcgg cgcgggatag gtaaaatcta tatgaaacga
     5341 tggtgagtgt caaactttgg gctctgtgct agctttttgt ggatcggagg acacattcca
     5401 tggccagcac ctggttccgg gagctcgggg cgcccgtcac ggccccgaaa accccgcgcc
     5461 cctgccggac
//
LOCUS       DASPKG010000058         4303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1716446, whole genome shotgun sequence.
ACCESSION   DASPKG010000058 DASPKG010000000
VERSION     DASPKG010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716446"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..564)
                     /locus_tag="VEA36_02495"
     CDS             complement(196..564)
                     /locus_tag="VEA36_02495"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HYF29208.1"
                     /translation="MKKLGYIALAAALPLFAFAQGTFEGTRGVQQLAISVIQFINGIL
                     VPLIFALAFIVFIWGVFQYFIAGGHDEEKKESGKSLMLYGLIGFFVMVSVWGLVNILV
                     TSFNLNKQQPSVPEAATQNI"
     gene            complement(646..1125)
                     /locus_tag="VEA36_02500"
     CDS             complement(646..1125)
                     /locus_tag="VEA36_02500"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HYF29209.1"
                     /translation="MKFLRYLIPAGLLLPLFASAQSSIDDNPLAKFLRAIIEFINDIV
                     VPLVFALAFVAFLWGIFQYFILGGADEEKRAEGRSFAIWGIIAFFVMVSIWGIVNLLV
                     SSFSFDERAPNTPTFGEPRQRSGDLDLGAPYDSKKCPPDTECIPGTSIPAGHEDVGL"
     gene            complement(1128..1376)
                     /locus_tag="VEA36_02505"
     CDS             complement(1128..1376)
                     /locus_tag="VEA36_02505"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29210.1"
                     /translation="MSFRELVDGTIVPIIDGAVIPLLYALAFIMFLVGIVRYFFFEGE
                     ESREKGKQLVLWGIIGFVVISTLWGIVKLLLATFNIAS"
     gene            complement(1373..2122)
                     /locus_tag="VEA36_02510"
     CDS             complement(1373..2122)
                     /locus_tag="VEA36_02510"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HYF29211.1"
                     /translation="MTSRLLILALFVSSILVPAEALAQTRASYTFTTTQSQIGRYWIE
                     AYRWDEANKCTSLDLLSYSTKQACDAALGAAQNNNNGWKYFGKCEKIDQTSYTYTVDV
                     RMCPGTISKYSLKPVEGTALPGGTPGGPTDPGGTPGGPTDPGGTPGGPTDPGGTPAGP
                     TGGLTNPIKFDSLEQLLEAVLAAIVRIGTIVLTLALVYVGFLFVVAQGNEEKIRNARS
                     ALLWTVIGGLILLGASALGQVIKATVNSIQP"
     gene            complement(2119..3426)
                     /locus_tag="VEA36_02515"
     CDS             complement(2119..3426)
                     /locus_tag="VEA36_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYF29212.1"
                     /translation="MANLRPFQVVILAVFGIAALLGLVLFATFQGFGRTEQDIGTVVI
                     WGTLSKRQVDPALTELKTLHKEFAGVSYVEKSPDTLDADIANAIASGSGPDLVLLNQE
                     HLVAERARLNVIPSASISERVYRDTYLPIFDIYLTEGGTYGIPLLVDPLVLYYNRSAL
                     ENAGVVTPPATWEAIAGLAPSLTRLTDAQTVSRSTIGMGTYENVTNAHALLSTLFFQA
                     GQTITARTAQGIRSTLGTEAGSALGVPTAESALTFYTEFANPGKTVYTWNRSLPTSRL
                     AFATGDVVFYPGFASERTTIAATNPNLTFDVAAIPQPGTASTRVTYGRAYALVIPKAS
                     RNAGGAYETARALAAADVLPTLARTTGMAPGVRSLLTPLTTDLYEPVYYPQALIARGW
                     LSPRPAALDSVFAAMIGNVNSGRMKPREALDTADQALNAALLQ"
     gene            complement(3428..3958)
                     /locus_tag="VEA36_02520"
     CDS             complement(3428..3958)
                     /locus_tag="VEA36_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYF29213.1"
                     /translation="MHFSDPKANVLQIGLKEGMKVGDLGTGAGLAALTAAHLVGGTGH
                     VYAVDIQEDLLKRVRDAAHEQGLRNLSTIWGDFEKLGGTTLKDHALDAVILSNTLFQL
                     EHREGALAEIKRILKPGGRLLIVDWAGAYGGLGPHPDHVVPEHAAEELFIGGGFHKTK
                     AFRAGPHHYSILFTAP"
     gene            complement(4060..>4303)
                     /locus_tag="VEA36_02525"
     CDS             complement(4060..>4303)
                     /locus_tag="VEA36_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HYF29214.1"
                     /translation="QGRGGSGIKTAEVTAKTGEIIGAKVLIGEEDEAEEIVVISKKGQ
                     VIRVTSAEIPTLGRATQGVRIMKMREGDSIASMVAL"
BASE COUNT          808 a         1289 c         1454 g          752 t
ORIGIN      
        1 cccggcggcc atgatcatct gcacgacgcc ccagacgaat accgcgaacg cgatgaccgc
       61 aaggagcgtg acgagggggg tgaggatctc gcgggtcacg gcggtgatga agttgtcgaa
      121 ggggttcgat tccatgcttc agctaagcat agcaaaggcc cgtcctcgga cgggcctttg
      181 cggtggagaa ggacgctaga tgttctgcgt cgccgcttcg ggcaccgatg gctgctgctt
      241 attgaggttg aaggaggtca cgaggatgtt cacgagcccc cagaccgaca ccatcacgaa
      301 gaagccgatg aggccgtaga gcatgaggct cttgccggac tccttcttct cttcatcgtg
      361 gccgcccgcg atgaagtact ggaacacgcc ccagatgaac acgatgaacg caagggcgaa
      421 gatgagcggc acgaggatgc cgttgatgaa ctgaatgacg ctgatggcga gctgctgcac
      481 gccgcgggtg ccctcgaagg ttccctgcgc gaatgcgaag agtgggagcg cagcggcgag
      541 cgcgatataa ccgagtttct tcatagatgg tgattagagg tacctctata ggatagaacg
      601 ggtaggaacc tcgtcaatag aggctttggt tctgtgtgga aaacgctaga gtcctacgtc
      661 ttcgtgcccc gcaggtatgc tcgtcccagg aatgcactcg gtatccggcg ggcacttctt
      721 tgagtcgtat ggggcaccga gatccaagtc tcctgatcgc tggcgcggct ccccaaaggt
      781 cggggtgttc ggtgcccgtt cgtcgaagct gaacgagctc acgagaaggt tcacaatgcc
      841 ccagatcgag accatcacga agaacgcgat gatgccccag atcgcgaagc tgcggccttc
      901 ggcccgcttc tcctcgtccg cgccgcccag gatgaagtac tggaagatgc cccagaggaa
      961 ggcaacgaag gcgagcgcga acacgagcgg caccacgata tcgttgatga actcgatgat
     1021 ggccctcagg aacttcgcga gcgggttatc gtcgatggac gactgcgcag atgcgaagag
     1081 cgggaggagg agcccggcag ggatgaggta gcgtaggaat ttcatagcta gctggcgatg
     1141 ttaaaggtag caaggaggag cttcacgatg ccccacaggg tcgagatgac cacaaaccca
     1201 atgatgcccc agaggacgag ctgcttcccc ttctcccttg actcctcgcc ctcgaagaag
     1261 aagtaccgca cgatgccgac gaggaacatg atgaaggcga gcgcgtagag gagcgggatg
     1321 accgcgccgt cgatgatggg aacgattgta ccgtcaacta attccctgaa gctcatggct
     1381 ggatgctatt cacggtcgcc ttgatgacct ggccgagcgc tgacgcgccg aggaggatga
     1441 ggccgccgat gacggtccag aggagcgcgc tgcgcgcgtt ccggatcttc tcctcgttcc
     1501 cctgggcgac cacgaagagg aagcccacgt acacgagggc gagcgtgagg acgatcgtcc
     1561 cgatgcgcac gatggccgca aggacggcct cgagcaattg ctcgagggaa tcgaacttga
     1621 tggggttcgt gagtccaccg gtcggcccag cgggcgtacc gccgggatcg gtcggtccac
     1681 cgggcgtacc gccgggatcg gtcggtccac cgggggttcc tccaggatcg gtcggcccgc
     1741 cgggggttcc tcctgggagg gccgtgccct ctaccggctt caggctgtac ttcgagatgg
     1801 tccccgggca catccgcaca tccacggtat aggtatagga agtctgatcg atcttctcgc
     1861 acttcccgaa gtacttccat ccattgttat tgttctgggc ggcaccgagc gccgcatcgc
     1921 aggcctgctt ggtcgaatac gaaaggaggt cgaggctcgt gcacttgttg gcctcatccc
     1981 atcgataagc ttcgatccag tacctcccga tctgagactg cgtggttgtg aaggtatagc
     2041 tcgcgcgcgt ctgggcaagc gcctcggccg gaacgagtat agaggatacg aacagggcga
     2101 ggatgaggag tcgggacgtc attgcaagag cgcagcgttc agagcctggt cggcggtatc
     2161 aagcgcttcc ctcggcttca tgcggccgct gttcacattc ccaatcatag cggcaaagac
     2221 gctgtcgagg gccgcggggc gtggggacag ccagccgcgg gcaatcaggg cctgcgggta
     2281 gtagaccggc tcgtagaggt cggtggtcaa gggcgtgagg aggctccgga ccccgggcgc
     2341 catgcccgta gtgcgtgcca gggtcggcag gacgtcggcc gccgcaagcg cccgcgcggt
     2401 ctcgtacgcc ccgcccgcgt tcctcgatgc cttcgggatg acgagcgcat acgcccggcc
     2461 gtaggtcacg cgcgtgctcg ccgtccccgg ctgcgggatg gccgccacat cgaacgtgag
     2521 gttcgggttg gtggccgcga tcgtcgtgcg ctcggaggcg aagcccgggt agaagacgac
     2581 gtccccggtc gcaaacgcca agcgcgaggt cgggagcgag cggttccagg tgtagaccgt
     2641 cttgccgggg ttcgcgaact ccgtatagaa cgtgagcgcg gactcggcgg tcggaacgcc
     2701 aagcgcgctc cccgcttcgg tcccgagggt cgagcggatg ccctgcgcgg tccgggcggt
     2761 gatggtctgc cccgcctgga agaagagggt cgagaggagc gcgtgcgcgt tcgtgacgtt
     2821 ctcgtaggtg cccatgccga tcgtgctccg gctcaccgtc tgcgcgtccg tgaggcgcgt
     2881 caagctcggc gcgagccccg cgatcgcctc ccaggtggca ggtggcgtga cgacgcccgc
     2941 gttctcgagc gcgctccggt tgtagtagag gaccagggga tcgacgagaa gcgggatccc
     3001 gtacgtcccg ccctcggtga ggtagatatc gaagatgggc aggtaggtat cgcgatagac
     3061 gcgctcggag atggaagcag acgggatgac attgagacgc gcgcgctcgg cgacgaggtg
     3121 ctcctgattg aggaggacga ggtccggacc cgagccggaa gcgatcgcgt tggcgatatc
     3181 ggcatcgagc gtgtccgggc tcttctcgac gtaggagacg cccgcgaact ccttgtggag
     3241 cgtcttgagc tcggtcagcg ccgggtccac ctggcgcttg gacagcgtcc cccagatgac
     3301 gaccgtgccg atatcctgct cggtcctccc gaatccctgg aaggtggcga agaggacgag
     3361 gccgaggagc gcagcgatgc cgaagacggc gaggatgacg acctggaagg ggcggagatt
     3421 ggccatacta gggagcggtg aagaggatcg aataatggtg gggaccggcg cggaatgcct
     3481 tcgtcttatg gaatcccccg ccgatgaaga gctcctcggc ggcgtgctcc ggcaccacgt
     3541 ggtccgggtg cgggccgagg cccccgtacg cgccggccca atcgacgatg agcaacctgc
     3601 cgcccggctt caagatgcgc ttgatctccg ccagggcgcc ctcgcggtgc tcgagctgga
     3661 acaaggtgtt cgagaggatg accgcatcga gcgcgtggtc cttgagcgtc gtgcccccga
     3721 gcttctcgaa gtccccccag atggtggaga ggttccggag gccctgctcg tgagcggcgt
     3781 cccggacgcg cttcaggagg tcctcctgga tgtccaccgc gtacacgtga ccggtccccc
     3841 cgactaggtg cgcggcggtc aaggcagcga gccccgcgcc cgtgccgagg tcgccgacct
     3901 tcatcccttc tttcagcccg atctgcagta cgttcgcctt cgggtcggaa aagtgcatgc
     3961 cttaaaaata gcatgggggt acgcgtgcga tggaccaatc aacaaataat agaaagaaga
     4021 gcgcgcccga agggacgcgc tccgcctgga tcgagtggat tagagcgcca ccatcgacgc
     4081 gatcgaatct ccctcccgca tcttcatgat gcgcacgccc tgggtggcgc gccccaaggt
     4141 cgggatctcc gcggacgtca cccggatgac ctggcccttc ttcgagatga ccacgatctc
     4201 ctctgcctcg tcctcctcgc cgatcaggac cttcgccccg atgatctccc cggtcttcgc
     4261 ggtcacctcc gcggtcttga tgcccgagcc cccgcggccc tgg
//
LOCUS       DASPKG010000059         4282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1853766, whole genome shotgun sequence.
ACCESSION   DASPKG010000059 DASPKG010000000
VERSION     DASPKG010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853766"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..596)
                     /locus_tag="VEA36_02530"
     CDS             complement(159..596)
                     /locus_tag="VEA36_02530"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYF29215.1"
                     /translation="MEEQKRRILEFLKAHRLTVIATVDTEHNRPEAAVIAFAEKESLD
                     LIFGTSNKTRKYRNIQNNPEVSFVIGWSDELGTVQYEGIATELSGDEAIEHGTILADK
                     NENARIFLSKADQRYFLVKPSWIRMVDKSVFPEERFELGPGEL"
     gene            complement(615..896)
                     /locus_tag="VEA36_02535"
     CDS             complement(615..896)
                     /locus_tag="VEA36_02535"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYF29216.1"
                     /translation="MNTEELKKKLTGEGFVHIYEWEDAPGTEYPAHAHQGAVSMYILA
                     GGLTFSFLDAEITLTTGDRFDVPIGKEHTAKVGPEGCTFLVGEMIEGDS"
     gene            complement(893..1213)
                     /locus_tag="VEA36_02540"
     CDS             complement(893..1213)
                     /locus_tag="VEA36_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29217.1"
                     /translation="MEKRYKIVVYVPESHAERLREAMGNAGAGRIGNYSHCTFTVKGT
                     GRFRPEEGADPAVGEIGKLEETEEERIETVCTGDALQGVLQAIRDVHPYEEPATDVYP
                     IEII"
     gene            complement(1227..1973)
                     /locus_tag="VEA36_02545"
     CDS             complement(1227..1973)
                     /locus_tag="VEA36_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29218.1"
                     /translation="MTRGRTIAICRELLAAYQSGKLGQTVMPEDSNPGFDTSEPEARL
                     SYFTLPMALNYQRDSYRLWEGALKTYQDPETQFVFSVAELSTRSEDDVRAALMKYKVA
                     LQPNKHVQTWRTIARTVHERWGSFAGLMAASDHDFLKLRQLVQVEHKKEFPYLSGPKI
                     FNYWAFILSTYGGVALANRSFIDIAPDTHITQCSVRLEVITAEEAITLSRDELSERWR
                     ALLAGSGIDPIDMHPPLWFWSRNGFLFEER"
     gene            2031..2483
                     /locus_tag="VEA36_02550"
     CDS             2031..2483
                     /locus_tag="VEA36_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29219.1"
                     /translation="MAIKNLIRLVLVTALLLLIPLISMQFIEGMAWSPFDFIIAGVLL
                     FGTGLAYELISRRGATTAYRIATGLMVATALFLIWVNLAVGIIGSEDNPANTLYLGVL
                     IVLLLGAILANFKARGMSRTLFVAAAAQTAVPFIALTIWQPAITVEVL"
     gene            2605..2925
                     /locus_tag="VEA36_02555"
     CDS             2605..2925
                     /locus_tag="VEA36_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HYF29220.1"
                     /translation="MTTESISAGTVHALPADLRKTLTTDRAARTAWEDLTPLARNEWI
                     CWVTSVKKDETRKNHIKRLIEELKEGKRRPCCWIGCIHRKDKAISPSVRAVLARGTKA
                     PKKG"
     gene            2961..3323
                     /locus_tag="VEA36_02560"
     CDS             2961..3323
                     /locus_tag="VEA36_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29221.1"
                     /translation="MNSFNAFNRAPKEELKTRANPVEANDNDAASKGEAGPDPVLVEA
                     NVALRGIVNDLGAIEKPADRVPVFQRMFNGPEVVTDNPALIPFFDRIAVIEGKYGREA
                     VKEIATLYGADRMSDAAP"
     gene            complement(3331..>4282)
                     /locus_tag="VEA36_02565"
     CDS             complement(3331..>4282)
                     /locus_tag="VEA36_02565"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HYF29222.1"
                     /translation="WAPELHKVGGGYRVYFVARHKDTQELCIGVASSDKPAGPFQAAQ
                     APLLRGNKIDPHVFVEETGESYLYWKEDNNDFWRDTLTELLFEHPEFIPILFPSQRHQ
                     KGVSLAITLWPWMKRHNRAEHFFQTFLIEAVTRDFYAFEQRLRRLLQESEAVTGMVNR
                     ILTELKTPFFVQQLSGDGFSLIGEKIKVLENDQSWEAHVIEGMWVTKRNRTYYMFYAG
                     NNFATSDYAIGIASSESPLGPFKKKKEPFLGSTAKWLAPGHPSVAVGPDGKDRLFLHA
                     YFPDQVGYRQFRAMLAVKLSFKGEWPEIEGIEEIKLPEQL"
BASE COUNT          872 a         1295 c         1164 g          951 t
ORIGIN      
        1 ttcggcaaga aggccatcaa gcactggttc tgtaggacct gcggcaccca atcctatgcc
       61 gagggcgacg cgttcccgaa gatgatgatc aacgtgcggt gccttgatgg cgtcgatgtc
      121 acgacgctcg aggtcacaga gttcaacggg aaagatatct agagctcgcc cggaccaagc
      181 tcgaaccgct cttccggaaa tacgctcttg tccaccatcc gtatccagga tggcttcacc
      241 aggaaatacc gctggtccgc cttcgagagg aagatgcggg cgttctcgtt cttgtccgcg
      301 agtatcgtgc cgtgttcgat ggcctcgtcg cccgatagct cggtggcgat tccttcgtac
      361 tgaacggtgc cgagctcatc gctccagccg atgacgaacg agacctctgg gttgttctgg
      421 atattccggt atttccgggt cttgttcgag gtcccgaaga tgagatcgag gctctccttc
      481 tcggcaaacg cgatgacggc cgcttcgggg cggttgtgtt cggtgtccac cgtcgcgatg
      541 accgtaaggc ggtgtgcctt gaggaattcg aggatccgtc gcttttgctc ttccatggaa
      601 gtattagagc atgcctacga atcgccctcg atcatctccc cgacgaggaa ggtgcaccct
      661 tcaggcccga ccttcgccgt atgctccttc ccgatcggca cgtcgaagcg gtcgccggtc
      721 gtgagcgtta tctcggcatc taagaacgaa aacgtgagtc caccagcgag tatgtacatt
      781 gagactgctc cctgatgcgc atgcgcaggg tattcggtgc cgggcgcatc ctcccattcg
      841 tagatatgca cgaaaccctc tcctgtaagc ttcttcttga gttcctcggt attcatataa
      901 tctcgatggg atacacgtcc gtggccggct cctcgtacgg atgcacatcg cggatcgctt
      961 gcagcactcc ctggagcgca tcgcccgtgc agaccgtttc gattcgctcc tcctcggttt
     1021 cctcgagctt cccgatctct cccacggccg ggtccgcgcc ttcctcgggc ctgaaacggc
     1081 ccgtaccttt caccgtgaag gtgcagtgcg agtagttgcc gatccggccg gcgcccgcgt
     1141 tccccatcgc ctcgcgaagc cgctcggcat gggactccgg gacgtagacg acgatcttgt
     1201 agcgcttctc catggcccaa gtatagctac ctttcctcaa acaggaatcc gttccggctc
     1261 cagaaccaga gcggcggatg catatcgatg ggatcgatgc ctgagcccgc aagtaatgcg
     1321 cgccagcgtt cggagagctc atcccgcgaa agcgtgatcg cctcctccgc ggtaatgacc
     1381 tccaagcgga ccgagcactg cgtgatatgc gtatccggcg cgatatcgat gaagctacga
     1441 ttcgccagcg ccacgccgcc atatgtgctc agaatgaacg cccagtaatt gaagatcttg
     1501 gggccggaga gatagggaaa ctccttctta tgctcgacct gcaccagctg cctgagcttc
     1561 aggaaatcat gatcgctcgc cgccatcagc cccgcgaacg atccccaccg ctcgtgcacg
     1621 gtccgcgcga tcgtgcgcca ggtctgcaca tgcttgttgg gctggagcgc gaccttgtat
     1681 ttcatgagcg ctgcccgcac atcgtcctcc gagcgcgtcg agagctctgc gacactgaag
     1741 acgaactgtg tctcgggatc ctggtacgtc ttgagcgcgc cctcccagag cctatagctg
     1801 tcccgctggt agttgagcgc cataggaagc gtgaagtagc tcaggcgtgc ctctggctcg
     1861 gatgtatcga aaccaggatt tgaatcctcg ggcatcacgg tctgcccgag cttgccggat
     1921 tggtaggcgg caaggagctc cctgcagatt gcgatggtgc gcccacgggt catacctgaa
     1981 gtatacgatc aaggaacctg gggatgcccg agcgagttat cctattcact atggccatca
     2041 aaaacctcat ccgcctcgtg ctcgtgaccg cgctactcct cctcattcct ctgatcagca
     2101 tgcagttcat cgaggggatg gcctggagcc ctttcgactt catcatcgcg ggcgtgctcc
     2161 tcttcggtac cggccttgcg tatgagctca tctcccgcag aggcgcgacg accgcctatc
     2221 ggatagccac cggcctcatg gtggcgaccg ccctcttcct catctgggta aacctcgccg
     2281 tcggcatcat cgggagcgag gataatcccg ccaatacgct gtatctcggc gtactcatcg
     2341 ttctcctcct tggcgccatc cttgcgaatt tcaaggcacg cggtatgtcg cgtacgctgt
     2401 ttgtagctgc agccgcgcag acggcagtcc cgttcatcgc gcttaccatc tggcagccgg
     2461 cgatcaccgt cgaggtgctg taggtgctcg gggtgaatgc gctcttcatc gcgctttggg
     2521 tcggatcggg attgctgttc cggcgagcga gcctgagctg accgccaaat ctacctcgtt
     2581 gcggtaatga ggtaccattc gcgtatgacc acggagtcga tatctgccgg aacggtacat
     2641 gccctgcccg ccgatctacg caagacgctc accactgacc gggctgcgcg taccgcatgg
     2701 gaggatctca cgccgctcgc ccgcaacgaa tggatctgct gggttacttc cgtcaagaag
     2761 gacgagacga ggaagaacca tatcaagagg ctgatcgagg aactcaagga gggaaagcgc
     2821 cgtccctgct gctggatcgg ctgcatccac cgcaaggata aggcgatcag cccttccgtg
     2881 cgggccgtgc tcgcaagagg caccaaggca ccgaagaagg gatgagcctg cgttattatt
     2941 caccatataa ccctaacccc atgaattcat tcaatgcatt caatcgcgcg ccgaaggagg
     3001 agctgaaaac ccgcgcgaat ccagtcgagg caaatgataa cgacgcggcg tcgaagggcg
     3061 aggctgggcc cgatcccgtt ctcgtggagg caaatgtcgc attgcgcggc atcgtcaacg
     3121 atctcggcgc gatcgagaag cctgcggatc gggtgccggt cttccagcgc atgttcaacg
     3181 gacccgaagt cgtaacggat aatcccgcgc tcatcccctt cttcgataga atcgccgtca
     3241 tagagggcaa gtatggccgt gaagccgtta aggagatcgc gaccctgtac ggcgcagata
     3301 ggatgagcga cgctgctccc tgaacgcttt ctacaattgc tcaggcagct tgatctcctc
     3361 tataccctca atttccggcc attcaccttt aaaggagagc ttgactgcaa gcatggccct
     3421 aaattgacgg taccccacct gatcagggaa gtaggcatgg aggaacagcc tatctttgcc
     3481 atctggaccc actgccacag acggatgtcc gggagcaagc catttagcgg tactgcccaa
     3541 gaatggctcc tttttcttct tgaacggtcc gagcggggac tcgctcgatg caataccgat
     3601 agcatagtcg ctcgttgcaa aattattgcc cgcataaaac atgtagtacg ttctattcct
     3661 ctttgtcacc cacatcccct caataacatg cgcttcccac gactgatcat tctcaagcac
     3721 cttgatcttc tctccgatca acgagaatcc gtctccgctt agctgctgaa cgaagaaggg
     3781 ggtctttagc tcagtgagta tgcgattcac cattcccgtc actgcttccg attcctggag
     3841 caatctacgc aaccgttgct caaatgcgta gaaatcccgc gttaccgctt cgatgagaaa
     3901 cgtctggaag aaatgctctg cgcgattgtg ccgcttcatc cacggccata gggtaattgc
     3961 cagggatact cccttctgat gcctttgaga agggaagagt atcgggatga attctggatg
     4021 ttcgaacaga agttccgtta atgtgtctct ccaaaaatca ttgttatcct ccttccaata
     4081 caaatacgat tcccctgttt cctccacaaa gacatgggga tctatcttgt tgcctcgcag
     4141 gagcggcgct tgtgctgcct ggaacgggcc ggctggctta tccgatgacg cgacgccaat
     4201 gcacaattcc tgggtatctt tatgccgagc aacgaaatac acccggtacc cgcctcctac
     4261 cttatgcagt tccggtgccc ag
//
LOCUS       DASPKG010000060         4701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_369459, whole genome shotgun sequence.
ACCESSION   DASPKG010000060 DASPKG010000000
VERSION     DASPKG010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369459"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VEA36_02570"
     CDS             <1..687
                     /locus_tag="VEA36_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYF29223.1"
                     /translation="VLDDEGRQRRLNLFTELRRQFLGYDGRFCGFAESFINDSQTTAE
                     PCEYGLALFYHRSLPFFEFYHDFIFGEYKKAPPGGYAERRQNGPLPRNVQAVRLLGAD
                     GSTFVVCHLHGLWLRAGKMDSPERKIQQERLCAFLRRTVHREDKLVLCGDFNLLPESE
                     TFRALADEFGLRNLIVEHGINDTRTSFYKKPQRVADYMLVSPNVEVLSFEVPAEPEVS
                     DHRPLILECR"
     gene            complement(760..1419)
                     /locus_tag="VEA36_02575"
     CDS             complement(760..1419)
                     /locus_tag="VEA36_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYF29224.1"
                     /translation="MAEPMLTALFGRHPETVANVDDANYGNQTPLTPKGERQAEHLRR
                     WVKENRIEYIFHGPQDRCKKVFEGYDLGIPLEENPAFLERRRPSHTIGRKNDEVAEDL
                     AAMVEHYGVGPARWDEEVYFESVDMVELGLRRILYVHETLGHRRFFCLTSGLRARMFA
                     LRAIHGGLDPEYFKQAYDFTGVYNAGIMELSYGPVFRKPDQFNWRFEFASNHYLPEEL
                     R"
     gene            1505..1996
                     /locus_tag="VEA36_02580"
     CDS             1505..1996
                     /locus_tag="VEA36_02580"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYF29225.1"
                     /translation="MKPALMAIGIPGSGKTTVLKPLAEDYGLAYVNRDDIRAELLGDA
                     TDQSANRRVWEEANRRTLAALAAGQGVVLDSTFVEAWKRREMIELLREAGASPIVGVL
                     CLVPLGVARKRNAARERVVPEDVLEQMHAKLAKQPPTADEGFDALVTLDELTERLKTL
                     LPA"
     gene            complement(1962..2561)
                     /locus_tag="VEA36_02585"
     CDS             complement(1962..2561)
                     /locus_tag="VEA36_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HYF29226.1"
                     /translation="MSPRTILMLGRPGSGKSTQFELLKQALPSAHFLSFGTLVRAFMQ
                     QDHEAGRRARALLEAGKMLPKFLSTAVWAPYLFAHLLPDQDLVIEGAPRQPFEAESLD
                     SFLEFYGREEVIVINLEVPAEVAIARLVERGNAEGRADDAALESITSRQTWYEEETVP
                     VIEGMRAAARYRVLDIDGTQTIEDIARAITRAVTSSGAP"
     gene            complement(2639..3274)
                     /locus_tag="VEA36_02590"
     CDS             complement(2639..3274)
                     /locus_tag="VEA36_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29227.1"
                     /translation="MFLFVDSQGGVDFSRHEYVDHWEDKMYAVNRQIRSHWSKRQHQP
                     TVVVLLVDARHRVLIVKDGTWHLPEYPLTEGEDFLARAYRGMQEQAGLAPYALYPLHP
                     VLLPELPVITRAGNEPDEQGYRGRAYCAVALQLDEIPDAFALRGDHGNGPPLEGRWLS
                     PDRAAAQLIPCAGDTIERFTQVDRPALRALADLILAHRLQRPPESRSFTST"
     gene            complement(3357..>4701)
                     /locus_tag="VEA36_02595"
     CDS             complement(3357..>4701)
                     /locus_tag="VEA36_02595"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HYF29228.1"
                     /translation="TLPEGALVIADAVSDEALDIAGVKGGAASAITEASTDLYVSVCN
                     FDGPRIRKTAQALNLFTDASTRFQNKLAPALAAYGMRDILALIEDVAGGECIGVADVY
                     PSPEEPNAVTVPLGKVNGLLGSAYSMEEVAGAFERLGFAYTTTEDTFTVLPGFERRDL
                     VIPEDLVEEAGRILGYEAIPSIELPLQKTAPDQRRYRGIERLKDFLIERGYSEVSTQS
                     FAKEGEIELANPLQSDRPWLRPSLLPNLEEALHRAAQLAPRLVGPDADIRLFEIGNVF
                     TADGEFLVLSLGARALSGKLSPDLVKDHVAALEQELFLEPAKARYSLDASMVEINLAD
                     VNLEKLGEEYAPVEVRLGAYRPFSPYPFALRDIAVWTPEGTEESAVANLILSNAGELL
                     VRLDRFDEFTKDGRTSYAFRLVFESMERTLSDADLDPAMQRITDALNAADGFSVR"
BASE COUNT          801 a         1516 c         1598 g          786 t
ORIGIN      
        1 gtcctcgacg acgaagggcg gcagcggcgg ctgaacctgt tcaccgaact gcgccggcag
       61 ttcttggggt acgacggccg gttctgcgga ttcgcggaga gcttcatcaa cgacagccag
      121 acgaccgcgg agccctgcga gtacggcctc gcgctcttct accaccgatc gcttccgttc
      181 ttcgagttct accacgactt catcttcggc gaatacaaga aggcgccgcc cggcggatac
      241 gccgagcgcc gccagaacgg ccccctgccg cgcaacgtgc aggcggtgcg gctcctcgga
      301 gcggacggca gcaccttcgt ggtctgccac ctgcacgggc tgtggctgcg cgccgggaag
      361 atggactcgc cggaacggaa gatccagcag gagaggctct gcgccttcct ccgccggacc
      421 gtgcatcggg aggataagct ggtgctctgc ggggacttca acctgctgcc ggagagcgag
      481 acgttccgcg cgctcgccga cgagttcggg ctgcgcaacc tcatcgtcga gcacggcatc
      541 aacgataccc gcacgagctt ctataagaag ccccagcggg tcgccgacta catgctcgtg
      601 tccccgaacg tcgaggtgct ctcgtttgag gtgccggccg agcccgaggt ctcggaccac
      661 cggccgctca tcctcgagtg ccggtgacgg caacgatggg tcgcccggcg ggcggcccat
      721 cttttcgtat ataaaagctg acccccgccg cggcaggggt caccggagtt cctccggtag
      781 gtagtggttc gaggcgaact cgaaccgcca gttgaactgg tcgggcttcc ggaacaccgg
      841 gccgtacgag agctccatga tcccggcgtt gtagacgccg gtgaagtcat aggcctgctt
      901 gaagtattcg gggtcaaggc cgccatggat ggcccggagc gcgaacatgc gggcccggag
      961 ccccgaggtc aggcagaaga accgccgatg cccgagcgtc tcatggacgt agaggatgcg
     1021 cctcaggccc agctcgacca tgtccacgga ctcgaagtag acttcctcgt cccatcgcgc
     1081 cggtccgacg ccgtagtgct cgaccatcgc ggcgaggtcc tcggccacct cgtcgttctt
     1141 cctgccgatg gtgtggctcg gccgcctgcg ctcgaggaac gcggggttct cctcaagcgg
     1201 gatgcccaga tcatagccct cgaacacctt cttgcagcgg tcctgcgggc cgtggaagat
     1261 gtactcgatc cggttctcct tcacccagcg gcggaggtgt tccgcctgtc gctctccttt
     1321 cggggtcagc ggggtttgat tcccatagtt ggcatcgtcg acgttcgcca cggtctccgg
     1381 gtgccgcccg aagagcgcgg tcagcatggg ctcggccatg gccttccttt cttcgctcgt
     1441 gcccgacttc cgagtagacc gtacctaatt ggcggctcat ggtcaacgcg gtacacttcg
     1501 cccgatgaag cccgcgctca tggcgatcgg catcccgggt tccggcaaga cgacggttct
     1561 gaagccgctc gcggaggact acggcctcgc gtacgtgaat cgcgacgata tccgcgcgga
     1621 gctcctcggg gatgcgaccg accagtccgc caaccgccgg gtatgggagg aggcgaaccg
     1681 ccgcacgctc gcggcgcttg cggcaggcca gggcgtcgtg ctcgactcca ccttcgtcga
     1741 ggcctggaaa cgcagggaga tgatcgagct ccttcgcgaa gcgggcgcct cccccatcgt
     1801 gggcgtcctc tgcctcgtac cgcttggggt agcgcgaaag cgcaatgccg cgcgggagcg
     1861 cgtggtgccc gaggacgtgc tcgagcagat gcacgcgaag ctcgcgaagc agccgccgac
     1921 cgcggacgag ggcttcgatg cgctcgtgac gctcgatgag cttacggagc gcctgaagac
     1981 gttactgccc gcgtgatcgc tcgagcgatg tcctcgatgg tctgcgtccc atcgatatcg
     2041 aggacgcggt agcgggccgc ggcgcgcatg ccctcgatga ccggcacggt ctcctcctcg
     2101 taccacgtct ggcggctcgt gatgctttcg agcgcggcat cgtccgcgcg tccttccgca
     2161 ttcccgcgct cgacgagtcg cgcgatcgct acctccgccg ggacctcgag gttgatgacg
     2221 atgacctcct cccgtccgta gaattcgagg aaggaatcga gggactcggc ctcaaagggc
     2281 tggcgcggcg ccccttcgat gacgaggtcc tggtccggca agaggtgcgc gaacaggtac
     2341 ggcgcccata ccgcggtgct gaggaacttc ggcagcatct ttcccgcttc aaggagcgcg
     2401 cgggcgcgcc tccctgcttc atggtcctgc tgcatgaacg cgcggacgag ggtgccgaag
     2461 gagaggaagt gcgccgaagg cagcgcctgc ttcaggagct cgaactgcgt gctcttgccg
     2521 gagccggggc gcccgagcat gaggatggtc cggggactca tgccggcagt atacgcttct
     2581 cgctataaaa aacgacgccg cccggggagg gcggcgcgag cgcgacacgt ggtgcgcgct
     2641 acgttgaggt gaaagaccgt gactccggcg gacgttgcag ccggtgcgcg aggatgaggt
     2701 cggcgagcgc gcgcagcgcg ggccgatcga cctgcgtgaa ccgctcgatg gtgtcgccgg
     2761 cgcaggggat gagctgggcg gccgcgcgat ccggcgacag ccagcgaccc tcgagcggcg
     2821 gtccgttgcc gtggtccccc cggagcgcga acgcgtccgg gatctcgtcg agctggagcg
     2881 cgacggcgca gtacgcgcgc ccgcgatagc cctgctcgtc cggttcgttg ccggctcgag
     2941 tgatcacggg gagctcgggc aggagcaccg ggtgaagcgg gtagagcgcg tacggtgcga
     3001 gcccggcctg ctcctgcatg ccgcgatagg cgcgggcgag gaagtcctcg ccttccgtaa
     3061 gcggatactc aggcaggtgc caggtaccgt ccttcacgat gagcacgcgg tggcgcgcat
     3121 cgacgagcag gacgacgacc gtcggctggt gctgacgctt cgaccagtgg gacctgatct
     3181 gccgattgac ggcgtacatt ttgtcttccc aatggtcgac gtactcgtgt ctcgaaaagt
     3241 cgacgccccc ttggctatcg acgaacaaaa acatggtgtg gctctccaag aaaaacacag
     3301 ggcgtgccgc acccccgcgt tcaggatacc ctcatgaaaa aagaagtaaa gcgcgtctac
     3361 cgcaccgaga acccatcggc ggcattgagc gcatccgtga tgcgctgcat ggccggatcg
     3421 aggtcggcgt ccgagagcgt ccgctccatg gactcgaaga cgagccggaa ggcgtaggag
     3481 gtgcggccat ccttcgtgaa ctcgtcgaag cgatcgaggc ggacgaggag ctcgccggcg
     3541 ttactgagga tgaggttcgc gacggcggat tcctccgttc cctcgggcgt ccataccgcg
     3601 atgtcccgga gcgcgaaggg atacgggctg aacggccggt atgcgccgag ccgcacttcg
     3661 accggcgcgt attcctcgcc gagcttctcg agattcacgt ccgcgaggtt gatctccacc
     3721 atggacgcgt cgagcgagta gcgggccttc gcaggctcga ggaagagctc ctgctcgagc
     3781 gcggccacat ggtccttcac gaggtccggg gagagcttcc cggagagcgc gcgggccccg
     3841 agcgaaagca cgaggaattc cccgtccgcg gtgaagacgt tcccgatctc gaagaggcgg
     3901 atatcggcgt ccgggccgac gagccgcggg gccagctggg cagcacgatg aagcgcctcc
     3961 tccaggttcg gcaggagcga gggccggagc cacggccggt ccgactgcaa gggattggcg
     4021 agctcgatct ccccttcctt cgcgaaactt tgggtggaga cctcggaata tccccgctcg
     4081 atgaggaagt ccttcagccg ctcgatgccc cggtaccggc gctggtcggg agcggtcttc
     4141 tggagcggga gctcgatcga aggaatcgcc tcatagccga ggatgcgccc tgcctcctcg
     4201 acgaggtcct ccgggatgac gaggtcgcgt cgctcgaagc cagggagcac cgtgaaggta
     4261 tcctcggtcg tcgtatatgc aaagccgagg cgttcgaagg cacccgccac ctcctccatt
     4321 gaatacgcgg agccgaggag gccgttcacc ttcccgagcg ggaccgtgac ggcgttcggc
     4381 tcctcaggcg aaggatacac gtcagcgacg ccgatacact ccccgcccgc gacgtcctcg
     4441 atgagcgcga ggatgtcgcg catgccgtag gcggcaagcg caggcgcgag cttgttctgg
     4501 aagcgggtcg aagcatcggt gaagaggttc aaggcctgcg cggtcttacg gatgcgggga
     4561 ccatcgaagt tgcagacgga gacgtagagg tcggtcgagg cttccgtgat cgcgcttgct
     4621 gcccctccct taacgcccgc gatgtcgagc gcctcgtccg agaccgcgtc agcaatcacg
     4681 agcgcgccct cggggagcgt a
//
LOCUS       DASPKG010000061         5837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_910003, whole genome shotgun sequence.
ACCESSION   DASPKG010000061 DASPKG010000000
VERSION     DASPKG010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910003"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VEA36_02600"
     CDS             <1..216
                     /locus_tag="VEA36_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW motif-containing protein"
                     /protein_id="HYF29229.1"
                     /translation="AILMKRMKADAPKHRIDLVKDDPVVIADGPFKDLEGKVGEIDEE
                     RGKVKVMVAMFGRETPVELDFLQVRKL"
     gene            235..657
                     /gene="rplK"
                     /locus_tag="VEA36_02605"
     CDS             235..657
                     /gene="rplK"
                     /locus_tag="VEA36_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HYF29230.1"
                     /translation="MAKVVKKIKLQIPAGKATPAPPVGTVLGPAGVNIGDFVNKFNEA
                     TKDRMGTIVPVEMTVYDDRTFSLVYKVSPMSRLILKALGVEKGSGKAPLASVGTLSRA
                     QIREIAETKLPDLSAGSVEAAEKTVEGTARSMGVKIGS"
     gene            723..1568
                     /locus_tag="VEA36_02610"
     CDS             723..1568
                     /locus_tag="VEA36_02610"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase"
                     /protein_id="HYF29231.1"
                     /translation="MSDLQSHPNTRCVRKACDALGILYKTHDAYGNFVAVGLDPIKYF
                     VNASAPFNNGAIDKICRDKAFSYRLLKDAVRTPRTKGYFDPYPEDPQYQGYVVEQSYE
                     AIADAIVQEFSLPVIVKMNSGLKGRNVYLCEDRSEVLAAIETIFNHKLPSSDYVALAQ
                     EYVPPTREYRTVVFKGEVVLVYEKDLSDATFTGNLSPFHYEGARAVPVEDAVVTGRIQ
                     GAVDGIFPVLDLEFGGIDLIEDEAGELHVIELNTHPGFSYFVRDNGDQALVAMYEYIL
                     RQLRR"
     gene            1579..1941
                     /locus_tag="VEA36_02615"
     CDS             1579..1941
                     /locus_tag="VEA36_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29232.1"
                     /translation="MKIFIAHASNFDFKSKVYEPLRNSALNEVHEFIFPQEQGVEVIT
                     KDVIKGIDLLIFDASVPSTGAGIELGWADAFSKRVICIHEQGARISSSIPLVTDTVIE
                     YSDPEDLVRKLTEALDQA"
     gene            complement(1916..2416)
                     /locus_tag="VEA36_02620"
     CDS             complement(1916..2416)
                     /locus_tag="VEA36_02620"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HYF29233.1"
                     /translation="MQDISLVVALGHNNVIGKDNGLLWHLPDDLKRFKMLTLGHPVIM
                     GRKTWESLPERVRPLPGRTNIVVTRDENYRADGALVARSIEDAFELAKASPGSEEICV
                     IGGGEIYRLALPHATRLYLTLVDDPTPGTVTFPDYAEFTKEVEREEHSESGINYTWLR
                     LDRELP"
     gene            complement(2429..2887)
                     /locus_tag="VEA36_02625"
     CDS             complement(2429..2887)
                     /locus_tag="VEA36_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HYF29234.1"
                     /translation="MAQEQTKEEIGALLARFPVCITYVPVGTEVDFYTFCTIPETTTI
                     YEIKPDTSLDPREVVAHAIETVQGRIAAILIPGRRFDASGTRHGKGAGWYDRFLAQVP
                     ASWTRIGFCFDDQFSDEPLVRESWDEPMDYVVVVPRDGRDPVLYTTGARN"
     gene            complement(2857..3567)
                     /locus_tag="VEA36_02630"
     CDS             complement(2857..3567)
                     /locus_tag="VEA36_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29235.1"
                     /translation="MALSDRRILEEMKKGDIVISPFSEEQLATSSYDVTLGEWFYREQ
                     PTKYNHALYNIWSREHMEHVWGADKVERAVLASEAFKKYNFEYDGIRPDDKVIVLRPG
                     ETILAHTNEFIGGKDHITTMMKARSSMGRNFIEVCKCAGWGDVGYVNRWTMEITNNSK
                     NYIIPLVVGRRIAQIIFFETGPILERDYTKSGKYASSTNLAELKRNWKPESMLPRLWA
                     DKDIKRVQPWHKNKPKKK"
     gene            complement(3578..4438)
                     /locus_tag="VEA36_02635"
     CDS             complement(3578..4438)
                     /locus_tag="VEA36_02635"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HYF29236.1"
                     /translation="MERAYLDTLKQIMETGTDREDRTGTGTRAIFGVQMRFNMADGFP
                     AVTTKKLAFKAVKSELLWFLEGSNDDNRLKELNGTEKTIWTDNAEADYWKPHAKFPGD
                     LGQIYGVQWRSWRTADGQTVDQIANVITKIKNSKESFKRRLIVTAWHPGELDYDEQQP
                     PAAALCPCHILFQFFVADGKLSLQMYQRSGDMFLGVPFNIASYSLLLHMVAQATDLVP
                     HEFVHVIGDAHIYKNHFDAVKEQLSREPLAFPKLWLNPEIKDLFTFTMDDIKLEGYES
                     HAPIKAAMAV"
     gene            4460..5149
                     /locus_tag="VEA36_02640"
     CDS             4460..5149
                     /locus_tag="VEA36_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HYF29237.1"
                     /translation="MKKGVFIVLDGNDGSGKATQTELLNQALAEKGIESVKLDFPAYD
                     RNLFGALVGECLAGKHGDFLHLDPKIASPLYALDRLESTPSIKEALSAGKVVLADRFA
                     SSNQIHQGGKIEDEAEREAFLLWLEQMEHEVLGIPRPDAIIYLKVPVETSLALLTEKR
                     AQKNAALGEAARDMVEEDRRYLERSHETANWLASREANWHIIDCAENGGMRTREDIHA
                     EVLIIIDSLVS"
     gene            5151..5570
                     /locus_tag="VEA36_02645"
     CDS             5151..5570
                     /locus_tag="VEA36_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005554973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HYF29238.1"
                     /translation="MKIYFAGSIRGGRDDQKLYEEIIAEVGAYGTVLTEHIGHLSLTD
                     GGETHITNEEIFARDMAWVREADAIVAEVSTPSLGVGYELGQAVALGKPILCLFRPQE
                     GKRLSAMVAGNPDLRIYEYQSREDAARILKEFFANFT"
     gene            complement(5567..>5837)
                     /locus_tag="VEA36_02650"
     CDS             complement(5567..>5837)
                     /locus_tag="VEA36_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29239.1"
                     /translation="EGRFGTRQRVFFAVADQVEGPYTSLGPVLLPPGEGWESGENGHA
                     SAWVKDDELYLFYQARSKEVEHAQANDWRYGIAVYRIADIVGTSR"
BASE COUNT         1173 a         1641 c         1801 g         1222 t
ORIGIN      
        1 gccatcctca tgaagcgcat gaaggccgac gcgccgaagc accgcatcga cctcgtgaag
       61 gacgacccgg tcgtcatcgc tgacgggccc ttcaaggacc tcgagggcaa ggtgggcgag
      121 atcgacgagg agcgcggcaa ggtgaaggtc atggtcgcca tgttcggccg cgagactccc
      181 gtcgagctcg atttcttgca ggtgcgcaaa ctctaaggta ggttatagcg acatatggca
      241 aaagtagtta agaagatcaa actccagatc ccggcaggga aggcgacgcc ggcgccgccg
      301 gtgggtaccg tgcttggtcc tgcgggcgtg aacatcggcg atttcgtgaa caagttcaac
      361 gaggcgacca aggaccgcat ggggaccatc gtgccggtcg agatgaccgt ctatgacgac
      421 cgtaccttct ccttggtcta caaggtctcc ccgatgtccc gcctcatcct caaggcgctc
      481 ggcgtcgaga agggctccgg caaggcgccg cttgcgtccg tgggcacgct ttcgcgcgcg
      541 cagatcaggg agatcgcgga aaccaagctc ccggaccttt ccgcaggctc ggttgaggct
      601 gccgagaaga cggtggaggg cacggcgcga tcgatgggcg taaagatcgg tagctagttt
      661 ctcgtatcag aacgaccccg ccggcagtag tcagcggggt cgttctcctt tacagtctcc
      721 ctatgtccga tctccagtca catccgaaca cgcgctgcgt gcgcaaggcc tgcgacgcgc
      781 tcggcatcct gtataagacg catgatgcat acggcaattt cgttgcggtc gggctcgatc
      841 cgatcaaata tttcgtgaac gcatcggcgc ccttcaataa cggcgcgatc gataagatct
      901 gcagggataa ggccttctcc taccggctcc tcaaggatgc ggtgcgtact ccacgcacga
      961 aggggtactt cgatccgtat cccgaggatc cgcagtatca ggggtacgtt gtggagcaga
     1021 gctatgaagc gatcgcggac gcgatcgtgc aggagttctc gttgccggtg atcgtgaaga
     1081 tgaactcagg gctcaagggg agaaatgtgt atctctgcga ggaccgttcc gaagtgctcg
     1141 cggcgatcga gacgattttc aaccataagc tcccgtcctc agactatgtc gcgcttgcgc
     1201 aagagtacgt gccgccgacg cgcgaatacc ggaccgttgt gttcaaaggt gaagtagtgc
     1261 tcgtgtatga gaaggatctc tcggatgcga cgttcaccgg caacctgagt cctttccact
     1321 acgagggtgc acgcgccgtg ccggtggaag atgcggtggt aacgggacgg atacaggggg
     1381 ccgttgatgg catcttcccg gttctcgacc ttgagttcgg cggtatcgac ctgatcgagg
     1441 atgaggcagg ggaactccat gtcatcgagc tgaacacgca cccaggcttc agctatttcg
     1501 tgcgggacaa cggcgatcag gcgctcgtgg cgatgtatga gtacattttg cggcaactca
     1561 ggcgctaaat ataatcatat gaaaatattc atcgcacatg cgagtaactt cgatttcaag
     1621 agcaaggttt acgaaccgct ccgtaactca gcacttaatg aggtacatga gttcatattc
     1681 cctcaggaac agggggtaga ggtgattact aaggatgtca ttaagggaat cgatctactt
     1741 atctttgatg catctgtacc ctcaacgggc gctggcattg agctgggatg ggcggatgcg
     1801 ttcagtaagc gtgtgatatg cattcacgaa cagggagctc gcatctcaag ctcgattccg
     1861 ctcgtaactg acacggtcat tgaatactcg gatcctgaag acttggtacg taagcttacg
     1921 gaagctctcg atcaagccta agccaggtgt agtttatacc actctccgag tgttcttcgc
     1981 gctccacttc tttcgtaaat tcagcgtagt cggggaacgt gactgtgcct ggcgtggggt
     2041 catcaacgag ggtgagataa aggcgggtag cgtgggggag ggccaagcgg taaatctcac
     2101 ccccgccgat gacgcagatc tcctcggaac ccggtgatgc tttcgccaat tcaaacgcat
     2161 cctcaattga acgggcgacg agtgcgccgt cggcacggta gttctcatca cgagtcacga
     2221 cgatattcgt gcggccggga agcgggcgga cccgttcggg aagcgattcc caggtcttgc
     2281 gccccataat cacgggatgg ccgagcgtga gcatcttgaa gcgcttgagg tcgtccggca
     2341 gatgccagag gaggccgttg tcctttccga tgacattgtt gtggccgagc gcgacgacga
     2401 ggcttatatc ctgcatagat agagcatatc agttacgggc gcccgtcgta taaagcaccg
     2461 gatctctgcc gtctctcggg acaaccacca catagtccat gggctcatcc catgactctc
     2521 ggacgagtgg ttcatcggag aactgatcat caaaacagaa cccgatgcgg gtccaggaag
     2581 cgggaacctg cgcaagaaag cggtcgtacc acccggcacc cttaccatgc ctggtaccgc
     2641 ttgcgtcgaa tcttcgtcca ggaatgagga tggccgctat ccgtccctgt acagtctcta
     2701 tcgcatgcgc gacgacttct ctcggatcaa gcgaggtgtc gggcttaatt tcatatatgg
     2761 tcgtcgtttc cggaatagtg cagaaggtat agaagtccac ttccgtccca acaggcacgt
     2821 aagtaatgca gacaggaaag cgcgccagaa gcgcgcctat ttcttctttg gtttgttctt
     2881 gtgccacggc tgcacccgtt tgatgtcctt gtccgcccag aggcgcggca gcatgctctc
     2941 gggcttccag ttgcgcttta gttccgcgag gttcgtggag gaggcgtact tcccgctctt
     3001 cgtgtagtcg cgctcgagga tgggaccggt ctcgaagaag atgatctgcg cgatgcggcg
     3061 gccgacgacg agcgggatga tgtagttctt ggagttgttc gtgatctcca tggtccagcg
     3121 gttcacgtac cccacgtctc cccagcctgc gcacttgcag acctcgatga agttgcggcc
     3181 catggagctg cgggccttca tcatggtggt gatgtggtcc ttgccgccga tgaattcgtt
     3241 cgtgtgcgcg aggatggtct ccccgggccg cagcacgatc accttgtcgt cggggcggat
     3301 gccgtcgtac tcgaagttgt atttcttgaa ggcctccgag gcaagcaccg cgcgctccac
     3361 cttgtccgcg ccccagacgt gctccatgtg ctcgcggctc cagatgttgt aaagcgcgtg
     3421 gttgtacttg gtcggctgct cgcggtagaa ccattcgccg agcgtcacgt cgtagcttga
     3481 ggtcgcgagc tgctcctcgc tgaacgggga gatcacgata tcgcccttct tcatctcctc
     3541 gaggatgcgt cggtcggaca gcgccatggt caggggatta tacggccatt gcggctttta
     3601 tgggagcgtg cgattcatag ccctcgagct tgatgtcgtc catggtgaat gtgaacaggt
     3661 ccttgatctc ggggttaagc cagagcttgg ggaaggcgag gggttcccgg gagagctgct
     3721 ccttcaccgc atcgaaatga tttttataga tatgcgcatc gccgatgacg tgcacgaact
     3781 cgtgcggcac gagatcggtg gcctgcgcga ccatgtgcag caagagcgag tacgatgcga
     3841 tgttgaacgg gacgccgagg aacatatcgc cgctcctttg atacatctgg agcgagagct
     3901 tcccgtcggc gacgaagaac tggaagagga tgtggcaggg gcacaacgcg gccgcgggcg
     3961 gctgctgctc gtcgtagtcg agctcgcccg ggtgccaagc ggtgacgatg aggcggcgct
     4021 tgaacgattc cttgctgttc ttgatcttgg tgatgacgtt cgcgatctgg tctaccgtct
     4081 gcccgtcagc ggtgcgccag ctgcgccact gcacgccgta tatctgcccg agatcgccgg
     4141 ggaatttcgc gtgcggcttc caatagtccg cctcggcgtt atcggtccag atggtctttt
     4201 cggtgccgtt cagttccttg aggcgattat cgtcgttgct cccttcgagg aaccacagaa
     4261 gctcgctctt cactgccttg aacgcgagct tcttggtcgt gaccgcgggg aagccgtcgg
     4321 ccatgttaaa gcgcatctgc acgccgaaga tggcgcgcgt cccggtcccg gtccggtctt
     4381 cccggtccgt accggtctcc atgatctgct tgagggtatc caaataggct cgttccatcc
     4441 gccccagtat actaggctca tgaaaaaggg ggtgttcatt gtgctcgacg gcaacgacgg
     4501 gtcggggaag gcgacccaga ccgagctctt gaaccaggcg cttgcggaga agggcatcga
     4561 gagcgtgaag ctggacttcc cggcctatga ccggaacctc ttcggggctc tcgtcgggga
     4621 gtgcctcgca gggaagcacg gggatttcct ccacctggac ccgaagatcg cctcgccttt
     4681 gtatgcgctc gatcgcctcg agagcacgcc ttccatcaag gaggcgcttt cagccgggaa
     4741 ggtggtgctt gcggaccggt tcgcgagctc gaaccagatc catcagggcg ggaagatcga
     4801 ggacgaggcg gagcgcgaag cgttcctgct ctggctcgag cagatggagc atgaggtgct
     4861 cggcatcccg cgcccggacg ccatcatcta cctcaaggtg ccggtggaga cgagcctcgc
     4921 gctcttaaca gagaagcgcg cgcagaagaa cgcggcgctc ggggaagcgg cgcgcgacat
     4981 ggtcgaggag gaccggcggt acctcgagcg cagccatgag accgcgaact ggctcgcctc
     5041 acgcgaggcg aattggcaca tcatcgactg cgcggagaac gggggcatgc gcacccgcga
     5101 ggacatacac gccgaggtcc ttataattat cgatagcctc gtctcttaat atgaaaatat
     5161 acttcgctgg ttcgatacgg ggcggccgtg atgatcagaa actctacgag gaaatcatag
     5221 cggaagtagg tgcatacggt acggtgctca ccgagcatat cgggcaccta tcgcttacgg
     5281 acggcggcga aacccacatt accaacgagg agatattcgc acgggatatg gcgtgggtcc
     5341 gcgaagcgga tgcgatcgtt gccgaggtaa gcacgccgag cctcggggtc gggtacgagc
     5401 ttggccaggc cgtcgcgctt ggcaaaccta tcctctgcct ctttcgtccg caggaaggga
     5461 agcggctctc tgccatggtg gctgggaatc cggacctcag gatctatgag tatcagtcac
     5521 gtgaggacgc cgcacgcatc ctcaaggaat tctttgcgaa cttcacctag cgagaagtgc
     5581 ccacaatatc tgcgatccga tacacggcga tgccgtaccg ccagtcgttg gcctgcgcat
     5641 gctcgacctc cttgctgcgg gcctgataga aaaggtagag ctcgtcgtcc ttgacccacg
     5701 cgctcgcgtg gccgttctcg ccggattccc atccttctcc gggagggagg agtaccggcc
     5761 caaggctcgt atacggccct tcgacctggt ccgcgaccgc gaagaacacc cgctggcgcg
     5821 tgccgaaacg cccttcg
//
LOCUS       DASPKG010000062         6370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_640300, whole genome shotgun sequence.
ACCESSION   DASPKG010000062 DASPKG010000000
VERSION     DASPKG010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640300"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VEA36_02655"
     CDS             complement(<1..420)
                     /locus_tag="VEA36_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29240.1"
                     /translation="MSRRIATGIDIGTHHTKIVVVEEVRTKEGPLLRIIGTGLSETRG
                     LRHGYVVDPEDAAASIRAARSQAEHVAGVPIKKGFLAIGGISLDEVRAQGETVISRAD
                     QEITDLDIEKTREAARLAAQPNFLNRTVLHEIPLEYRV"
     gene            complement(392..799)
                     /gene="ybeY"
                     /locus_tag="VEA36_02660"
     CDS             complement(392..799)
                     /gene="ybeY"
                     /locus_tag="VEA36_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HYF29241.1"
                     /translation="MDTRNFTRSRVPSFPYERAAKAVLPGWDISLVFAGETRAKQLNK
                     ALRGKDYVPNVLSYESGKKSGEIIICLTEAKRQAPSHDMKYPAFVGYLFIHGLFHLAG
                     ARHGTTMERKERALLARLINIPFTNVPTHRHRH"
     gene            complement(844..1101)
                     /locus_tag="VEA36_02665"
     CDS             complement(844..1101)
                     /locus_tag="VEA36_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29242.1"
                     /translation="MTNAVRVEVRRGKNESSASLIRRFSRRGQGLGLVRALRKRRYYS
                     RSKSKNVEHARALIRGERITEYNELVKLGKIDPAARRTKKR"
     gene            complement(1199..1642)
                     /locus_tag="VEA36_02670"
     CDS             complement(1199..1642)
                     /locus_tag="VEA36_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29243.1"
                     /translation="MPHLQSAKAGVAISLLGMGAIAYGLTIDSAMIDHPLLLLTAPVF
                     VLCMMMSHSLLGIRDFPAPRNFFEGAQFMVVGIAIIVPTMVALGMLMRWLDHMDPVVS
                     GVAMFRWAARAMIVIGGMVLPLGFVITAVNSVFAVTDRRYHAPAA"
     gene            complement(1743..2888)
                     /locus_tag="VEA36_02675"
     CDS             complement(1743..2888)
                     /locus_tag="VEA36_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HYF29244.1"
                     /translation="MSTVAHRPDEVLKRAHAIGTYYGFIPLSSLAAAKRGAKKEAMPD
                     AIAPEALDPVARDVAALLKHVRDAGIVPSLAQPLFLWHTNAASGRPSPKQITIQFHVL
                     GVEHAIADAVLIRAVRALVQDLAKGEPVLRLNSMGDRETRGRFARELTSFFRKQGATL
                     PADCMASARQGDVFTAAELLAASEDADELPCPTDHLSEASRKHFEGVLEYLEATDTPY
                     ELAPGLLSRGTSWSETCFEIRGEEGIRAWGSRYNDFARPFFKTHLPSVGAVVQVAVEG
                     KEVIAPLKDRKSPRFVFVHIGDEAKRECMKMADEFRRARIPMTQSIGIASLTEQMRHV
                     ETLNPPYLLIMGRKEALERSVILRERATHTETFIPLDHLVDMLKQVD"
     gene            complement(2885..3436)
                     /gene="lepB"
                     /locus_tag="VEA36_02680"
     CDS             complement(2885..3436)
                     /gene="lepB"
                     /locus_tag="VEA36_02680"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HYF29245.1"
                     /translation="MKFLGTAMKEILTFITLAVIIVIPIRLFVAQPFVVEGESMHPTF
                     ESGDYLIVDQLSYRFGSPKRGDPVVFRYPNDPSVFYIKRVIGLPGETVHIDKGKTSIT
                     KPDGTKLALNESYVVAEDATYTLDSTLGEDEYFVMGDNRPRSSDSRVWGPLPREDLMG
                     RAFIRLLPPATAGVLPGAVTEPQ"
     gene            3746..4507
                     /locus_tag="VEA36_02685"
     CDS             3746..4507
                     /locus_tag="VEA36_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYF29246.1"
                     /translation="MRTEELVLRNRAGERMPATLFRPDAASVGTAVILHGLGAWRNQP
                     LLLAVARLLADQGYAVLRFDEANGVTAPDGDFFRSTTTKYTQDVEDVLAYVNSAPWRS
                     GPMLLVGHSLGGLVAAWYAARHPDQVARLVLIAPAISWKTMWWKWLPLSLLWYVRGYR
                     LILGIEGKKFALSPLWWRDFFRYDAMAYAPQIPAPTLVISAEQDHTVGRPRDERALAR
                     RFPNGEHSMVSWTNHIFDEHVDEVVDTINRWLTSS"
     gene            4489..5088
                     /gene="pth"
                     /locus_tag="VEA36_02690"
     CDS             4489..5088
                     /gene="pth"
                     /locus_tag="VEA36_02690"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HYF29247.1"
                     /translation="MAYVILGLGNPGAEYTKTRHNAGRMVAEFLAAEEGFAFKEKKTA
                     QALVAEGTLGDEKVLIALPETQMNLSGKAAAALVKSKPAAKKLLVIRDELDMPLGTIK
                     MTMPGRGSGGHKGIESVMRALKTKDFVQMKIGISPATPTGKLRKPEAGEEVVAHVLGR
                     FSPKEEALLNKTLARAAEAARLFVTGSLGRACLVANTNA"
     gene            5175..5936
                     /locus_tag="VEA36_02695"
     CDS             5175..5936
                     /locus_tag="VEA36_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29248.1"
                     /translation="MFETAPQELLLALKDIYGLSFIPVPFETREELDGALTDAGAPLG
                     PWVDELWEEYTDYDSILAHDGQGNIHRFIWTMTFLVIRERRRDDLPGSDLMSREVLKE
                     RIEWPDGRVSERQYANTMSEKLRWRRAGVWIWTFRKWRPEVVRAVKEELGVCLTRSFL
                     GRPWLVRVMPDVHYYKDPETGASGAIVVKEHDPKRPKVQTHNRLFHFILKLHKRFWKA
                     AYREAKRRHDRLVKTMVHTWAPAEDRVRLPARVPP"
     gene            complement(6015..>6370)
                     /locus_tag="VEA36_02700"
     CDS             complement(6015..>6370)
                     /locus_tag="VEA36_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HYF29249.1"
                     /translation="DPRALFKVGDTVRVKVIEVKDGKVSLSVKALRENPWKIAADKYQ
                     KGQEVQGVVIKYNKHGALASIEEGVAGLVHISEFASPQELRDTLELGKTYPFTITLFE
                     PKDQRMTLSFTGKKK"
BASE COUNT         1156 a         1979 c         2069 g         1166 t
ORIGIN      
        1 cacccggtat tcgagcggta tctcatgcag caccgtgcga ttcaggaaat tcggctgggc
       61 cgcgagccgc gcggcctccc gcgtcttctc gatgtccagg tccgtgatct cctggtccgc
      121 ccgggagata acggtctcgc cctgcgcccg gacctcgtcg aggctgatcc cgccgatcgc
      181 gaggaagccc ttcttgatgg gtacgccggc gacgtgctcg gcctgggacc gcgccgcccg
      241 gatgctcgct gcggcgtcct ccgggtccac cacgtaccca tggcgcagcc cccgggtctc
      301 tgagagcccc gtcccgatga tccggagcag gggaccttcc ttggtacgca cctcttccac
      361 caccacgatc ttcgtgtggt gcgtgccgat atcaatgccg gtggcgatgc gtcgggacat
      421 ttgtaaaagg aatattgatg agacgggcca gtagcgcccg ctccttacgt tccattgtag
      481 ttccgtgacg cgctccggcc aaatgaaaga gtccgtggat aaagaggtag ccgacgaatg
      541 cggggtactt catgtcgtgc gacggtgcct gccgcttggc ttcggtgagg cagatgatga
      601 tctcgcccga cttcttcccg ctctcgtacg agagcacgtt cgggacgtag tccttaccgc
      661 gtagtgcctt gttgagttgc ttcgcgcgcg tctctcccgc aaacacgagc gagatatccc
      721 agcccgggag caccgccttc gcggcccgtt cgtacggaaa ggagggaacg cggctcctgg
      781 tgaagttccg agtatccatt acggggagta tataacgcgt aaagccccgt gaggggcttc
      841 ctgctagcgc ttcttcgttc ggcgcgccgc cgggtctatc ttcccgagct tcacgagctc
      901 gttgtattcg gtgatgcgct cgccgcggat aagggcgcgg gcatgctcca cgttcttgga
      961 cttcgagcgg ctgtagtagc ggcgcttgcg cagcgcgcga acgagcccga ggccctggcc
     1021 ccggcgggag aaacggcgaa tgagcgaggc cgaactctcg ttcttgccgc ggcgaacctc
     1081 tacgcgtact gcgttagtca tacgaggaag gatagcacag gtaggtaaat cctgtctaga
     1141 aacacaaacc cctcgggacg gaaagccgag ggggggggtg aaattcatca ggaagcggtc
     1201 aggcggccgg ggcgtggtac cggcggtctg tgaccgcaaa cacgctgttg acggcggtga
     1261 tcacgaagcc cagaggaagc accatgccac ctatgacgat catggcccgc gccgcccagc
     1321 ggaacatggc gactccggac accaccggat ccatgtggtc cagccagcgc atcagcatgc
     1381 cgagggccac catggttggc acgatgatgg cgatcccgac caccatgaac tgagcgcctt
     1441 cgaagaagtt cctcggcgcc gggaaatccc tgatgccgag aagcgagtgg ctcatcatca
     1501 tgcagagcac gaagaccggt gcggtcagga gcagcagagg atgatcgatc atggcgctgt
     1561 caatcgtcag gccgtaagcg atggcgccca tgccgagcag agagatggcg acgcccgcct
     1621 tggcactttg cagatgtggc atgtctttat tcctgtttgc ggccgtcagg ccaggtgcaa
     1681 tgaaactgaa atatacttta gcgcataatt agtaaaaagt caataccctc ggcggggctc
     1741 aactaatcta cctgcttgag catatcgacc agatggtcga gcgggatgaa ggtctcggta
     1801 tgggtggcgc gctcccggag tataacggag cgctccaagg cctccttgcg gcccatgatc
     1861 aagaggtacg gcggattcag agtctccaca tgccgcatct gctcggtcaa ggacgcgatg
     1921 ccgatggact gcgtcatcgg gatgcgggcc ctgcggaact cgtccgccat cttcatgcac
     1981 tcgcgcttgg cttcgtcgcc gatgtgcacg aacacgaagc gcgggctctt gcggtccttc
     2041 aggggcgcga tcacttcctt gccctccacc gcaacctgga ccacggcgcc caccgacggc
     2101 aggtgcgttt tgaagaaggg gcgcgcgaaa tcgttgtagc gggagcccca ggcacggatg
     2161 ccttcctcgc cccgtatctc gaagcaggtc tcgctccagg aggtcccgcg ggagagcagc
     2221 cccggcgcaa gctcgtaggg cgtgtcggtc gcctcgaggt attcgaggac gccctcgaag
     2281 tgcttgcggc tcgcctccga gaggtggtcg gtggggcacg ggagctcgtc cgcgtcctcg
     2341 gacgcggcaa ggagctcggc cgccgtgaac acgtcgccct ggcgggcgga ggccatgcag
     2401 tccgcaggaa gggtcgcccc ctgcttccgg aagaaggagg tgagctcgcg cgcgaacctt
     2461 ccgcgcgtct cacggtcccc catggaattc aatcgcagca ccggctcgcc cttcgcgagg
     2521 tcctgcacga gcgcacgcac cgcgcggatc aatactgcgt ccgcgatcgc atgctcgacc
     2581 ccgagcacat ggaactggat ggtgatctgc ttcggcgagg ggcggccgct cgcggcattg
     2641 gtgtgccaga gaaagagcgg ctgggccaga gacgggacga tgcccgcgtc cctgacgtgc
     2701 ttcaagagcg cggcgacatc gcgggcgacc gggtcgagcg cctcaggggc gatcgcgtcc
     2761 ggcatcgcct ccttcttcgc gccgcgcttt gcggccgcaa gcgacgagag gggaatgaag
     2821 ccgtaatacg tgccgatcgc gtgcgcgcgc ttcagtacct cgtcggggcg gtgcgctacg
     2881 gtactcattg cggttcggta acggcgccgg ggagcactcc tgcggtcgcg ggcggcagga
     2941 gccggatgaa ggcccggccc atgaggtcct cgcgcggcaa cgggccccag acgcgcgagt
     3001 cggagctccg ggggcggtta tcgcccataa cgaagtactc gtcctcgccc aaggttgagt
     3061 cgagggtgta ggtggcgtcc tccgcgacga cgtaggactc gttgagcgcg agcttcgtgc
     3121 cgtcgggctt cgtgatcgag gtcttcccct tatcgatatg cacggtctcg cccggcagcc
     3181 cgatcactcg cttgatgtag aagacggagg ggtcgttcgg gtagcggaaa acgaccggat
     3241 cgccgcgctt cggggacccg aagcggtagg agagctggtc gacgatcaga tagtcgccgc
     3301 tctcgaaggt gggatgcatg ctctcgccct cgacgacgaa cggctgcgcg acgaagaggc
     3361 ggatggggat gacgatgatc actgcgagcg tgatgaaggt gaggatctct ttcatcgccg
     3421 tcccgaggaa cttcatgtga gcgtattgta tgcttccgtt ttcgtaatgc aactctccgg
     3481 gaatagggaa aggccgctgc tccgtagcag cggcctccca agagcgcgac gacagtcggc
     3541 agccgaggct tggtgcgtct gcgctctcgc atgtgcgtgg atcgactacg cggctacggg
     3601 cacttcggga gcctcctcga cggcttcgcg cgcgtcgtca ggatgctcca ggctgtagag
     3661 cagtgggtcc atggcgtcct cccatctcaa gcgtactcct ccctcgcgcg gattgcgagg
     3721 ttatccactt tgctatcatg cgggcatgag aacggaagag ctcgtgctcc ggaaccgcgc
     3781 tggagagcgt atgcccgcga ccctcttccg tcccgatgcg gcatcggtcg gcaccgcggt
     3841 catcctgcac gggctcggcg cgtggcggaa ccagccgctg ctgctcgccg tcgcgcggct
     3901 cctcgcggac cagggatacg cagtgctgcg ctttgacgaa gcgaacggcg tgacggcgcc
     3961 cgacggcgat ttcttccgca gtacgaccac gaagtacacg caggacgtcg aggacgtgct
     4021 cgcgtacgtc aacagcgcgc cctggcgctc gggccccatg cttcttgtcg ggcactcgct
     4081 cgggggactg gtcgccgcct ggtacgccgc acggcatccg gaccaggtcg cgcgcctcgt
     4141 cctcatcgcg cccgcgatct cctggaagac catgtggtgg aagtggctgc cgctctcgct
     4201 cctgtggtac gtccgcggat accgcctcat tctcggcatc gaggggaaga agttcgccct
     4261 ctccccgctc tggtggaggg acttcttccg gtacgacgcc atggcgtacg ccccgcagat
     4321 cccggcgccc accttggtca tctcggccga gcaggaccat acggtcgggc gaccgcgcga
     4381 cgagcgcgcc ctggcgcggc gcttcccgaa cggggagcac tccatggtga gctggaccaa
     4441 ccatatcttc gacgagcacg tggacgaggt cgttgataca atcaaccgat ggcttacgtc
     4501 atcctagggc tcgggaaccc gggcgccgaa tacacgaaga cccgccacaa cgcgggacgc
     4561 atggttgcgg aattcctcgc agcggaggaa ggatttgcat ttaaggaaaa gaagacggcg
     4621 caggcactgg tcgccgaggg cacccttggc gacgagaagg ttctcatcgc gctccccgag
     4681 acccagatga atctctcggg gaaggcggcc gcagcgctcg tcaaatcgaa gcccgccgcg
     4741 aagaagctcc tcgtcatccg cgacgagctc gatatgccgc tcgggaccat caagatgacc
     4801 atgcccggcc ggggctcggg cgggcataag ggcatcgaat cggtcatgcg ggcgctgaag
     4861 accaaggatt tcgtgcagat gaaaataggc atctcacccg caacacccac gggaaagctc
     4921 cggaagccgg aagcggggga agaggtggta gcgcacgtcc tcgggagatt ctcgccgaag
     4981 gaggaggcgc tcctaaataa gacgctcgcg cgcgccgcag aggccgcacg actgttcgtc
     5041 acgggcagcc tcggacgggc gtgcctggtc gcgaacacca acgcatgaag tcttcatagc
     5101 gtgcgttgca gctctgaatg aagtagggca tagtgcgctc ggacaccgat gtcctttaac
     5161 ggaggagatg cgtcatgttt gagaccgctc ctcaggagct gcttctcgcg ctcaaggata
     5221 tttacggttt gtcattcatt ccggtaccgt ttgaaacccg ggaggagctt gatggcgcgc
     5281 ttacggatgc aggcgctccg cttggtccct gggtggatga actgtgggag gaatacaccg
     5341 attacgattc catcctcgcg catgacgggc aggggaacat ccaccgcttc atctggacga
     5401 tgacgttcct cgtcatccgg gagcgtcgtc gcgatgacct tcccgggtcc gacctcatga
     5461 gtcgcgaagt gctcaaagag cgcatcgaat ggccggacgg acgcgtatct gagcgtcagt
     5521 acgccaacac catgagcgaa aagctccggt ggcggcgcgc cggcgtctgg atctggacgt
     5581 tccgcaagtg gcgcccggaa gtggtgcgtg cggtcaaaga ggaactcggc gtgtgcctta
     5641 cgcgcagttt cctcggtcgt ccgtggctcg tccgcgtcat gcccgatgtc cattactaca
     5701 aggacccgga aaccggtgcg agtggcgcaa tcgtcgtaaa ggagcatgac ccgaagcggc
     5761 cgaaggtaca gacccacaat cggctcttcc acttcattct gaagctgcac aagcggttct
     5821 ggaaggcggc ctatcgcgag gcgaagcgcc gtcacgaccg cttggtcaag acgatggtgc
     5881 atacgtgggc tcctgcggaa gaccgcgttc gacttcctgc acgcgtccct ccgtagcccc
     5941 cggccagact cgccgggggc acccatttat ataccgaaac tgccctgcat gagccgggca
     6001 gtttccgctt caccttattt cttcttgccg gtgaaggaga gcgtcatgcg ctggtccttc
     6061 ggctcgaaga gcgtgatggt gaacgggtag gtcttgccga gctcgagcgt gtcgcgcagc
     6121 tcctgcggcg aggcgaactc cgagatgtgc accaggcccg cgacgccctc ctcgatcgag
     6181 gcgagcgcgc cgtgcttgtt gtacttgatc acgacgccct gcacctcctg gcccttctgg
     6241 tacttgtcgg cagcgatctt ccacgggttc tcccggagcg ccttcaccga aagcgatacc
     6301 ttgccgtcct tcacctcgat gaccttcacg cggaccgtat cgcccacctt gaagagggcg
     6361 cggggatcct
//
LOCUS       DASPKG010000063         3718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1723347, whole genome shotgun sequence.
ACCESSION   DASPKG010000063 DASPKG010000000
VERSION     DASPKG010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723347"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..83)
                     /locus_tag="VEA36_02705"
     CDS             complement(<1..83)
                     /locus_tag="VEA36_02705"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011081942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HYF29250.1"
                     /translation="MTTEVAIFGGGCFWCTEAVFKMLKGVVT"
     gene            106..1059
                     /locus_tag="VEA36_02710"
     CDS             106..1059
                     /locus_tag="VEA36_02710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HYF29251.1"
                     /translation="MKRYLFALVFLSAFFAMFSFLESRTVIFRTLVSSAKTAIAPETE
                     ARLLFVGDLMFDRHIRTVMERSGGEAVLGSVTPLLKDADLTIGNLEGPVTDNLSQSQG
                     TKVGDLTNMRFTFSPAVPKLLHDAGFDLVSIGNNHIQDFGVEGVRSTRAYLSSANISY
                     VGDPTGASTEPVVKEVNGITVAFVAYSDFVGGDADRAMQEVAGSTADVTVVLAHWGTE
                     YEAEPLPRVRKLAGDFAAAGADLIIGSHPHVIGAVEDIGTTRVYYSLGNFVFDQYFDQ
                     NVRCGLAVKATVRKGKSGVALAFEETKVGMRSDGATVLGCS"
     gene            complement(1056..1457)
                     /gene="msrB"
                     /locus_tag="VEA36_02715"
     CDS             complement(1056..1457)
                     /gene="msrB"
                     /locus_tag="VEA36_02715"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HYF29252.1"
                     /translation="MDEMPRTEEEWEQKLSPEEFRVLRERETEAPFSGEYVNNHEAGE
                     YRCKGCGNLLFTSAQKFDSGTGWPSFTDPAVAENVGTRPDDSYGMRRTEVYCKQCGGH
                     LGHVFNDGPPEAGGKRYCINSVCLAFERSEH"
     gene            1517..1978
                     /locus_tag="VEA36_02720"
     CDS             1517..1978
                     /locus_tag="VEA36_02720"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HYF29253.1"
                     /translation="MTTRTGLLFALLVLAALGIIDAWYLTLSAFQGAALVCDIGAALD
                     GCNIVAQSPYSKLFGLPLALYGVAFYGLVFALVAALFIATHHPLYQAAVVLGVLGLVA
                     SLGFLFIQFVLIKALCIYCVFSAVIAALICILSFVLFRKHAPGRQAAPMVS"
     gene            1985..3133
                     /locus_tag="VEA36_02725"
     CDS             1985..3133
                     /locus_tag="VEA36_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29254.1"
                     /translation="MKGTAVLLTLILLIPGVALASTVRTDRTLVISEPISDNAYLAGT
                     DVNVSAPLGGDLLSMGGTLTIAAPVAGDAFLAGGTIDVKRPVQGDVRAAGGRILIEDD
                     VAGDLVLAGGSVEVTGTASSTRVAGGNVRITGGATGPAVVYGGAVYLAGEFLGDVEVT
                     ASDRLMLAEGTRIHGTLRYNTPQQAGIPASAIIDGGVEYTGGSSYLPTVEEAKRFAVA
                     GAGVFFVASVIAAVIAAGLLAGLFPGLSERVTERTLAPSPRRFVLHTLLGFAIVVAAP
                     VLILFLLLSFVGTLVALMLALAYVFLLILGYLYAGIIAGAALARGLFKRSSVSWRFAV
                     LGMFVLYVIGVIPVFGKLVILVLSSAAIGAIAIIAYRFAFPRADDIEL"
     gene            3133..3600
                     /gene="bcp"
                     /locus_tag="VEA36_02730"
     CDS             3133..3600
                     /gene="bcp"
                     /locus_tag="VEA36_02730"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HYF29255.1"
                     /translation="MPAAGEVAPEFDLPDQEGRKHSLADYRGRYVLLYFYPKDDTPGC
                     TKEACAIRDADPDFSALDAVVLGVSADSVKSHKKFAQKYGLTFPLLADEDRTVVNAYG
                     VWGRKKFMGREYDGIFRTSFLIDPAGKIAKVYEGVKPDAHAAQVLADLRALQV"
BASE COUNT          563 a         1236 c         1165 g          754 t
ORIGIN      
        1 gttaccacgc ctttgagcat cttgaagacc gcctcggtgc accagaagca gccaccgccg
       61 aatatcgcca cctccgtcgt catatcccta ctgtatcatc agggtatgaa gcggtacctc
      121 ttcgcgctcg tcttcctctc ggccttcttc gccatgttct ccttccttga atcgaggacg
      181 gtgatcttcc gcacgctcgt ctcgagtgcg aagaccgcga tcgcgcccga gaccgaagca
      241 agactcctct tcgtcggcga cctcatgttc gaccgccata tccgcaccgt gatggagcga
      301 agcggcgggg aggccgtgct cggttcggtc acgccgctcc tcaaggatgc agatctcacc
      361 atcgggaacc tcgagggacc ggtaacggat aacctctcgc aatcccaggg aacgaaggtc
      421 ggggacctga ccaacatgcg cttcacgttc tcgcccgcgg ttcccaagct cctccatgat
      481 gccggcttcg atctcgtctc catcggcaac aaccatatcc aggacttcgg cgtggagggc
      541 gtgcgctcga cgcgggccta tctctcatcc gcgaatatct cgtatgtggg agacccgacc
      601 ggggcctcga ccgagcccgt cgtaaaggag gtgaacggca tcacggtcgc cttcgtcgcc
      661 tacagcgatt tcgtcggcgg ggacgctgac cgggccatgc aggaggtcgc gggaagcacc
      721 gcggacgtga ccgtcgtgct cgcgcactgg ggcaccgagt acgaggccga accgctgcca
      781 cgcgtccgga agctcgcggg agatttcgct gccgccggcg cagaccttat catcggctcg
      841 cacccgcacg tcatcggggc ggtcgaggac atcggtacca cccgcgtgta ttactcgctc
      901 gggaacttcg tcttcgacca gtacttcgat cagaacgtcc gctgcgggct tgcggtcaag
      961 gcaaccgtac gaaaagggaa gagcggggtg gcactcgcct tcgaggaaac gaaggtggga
     1021 atgcgcagcg acggcgcgac cgtgctcggg tgttcttagt gttcgctgcg ctcgaacgcc
     1081 aggcagactg agttgatgca gtagcgcttc ccgcccgctt caggcgggcc gtcgttgaag
     1141 acgtggccca ggtgcccgcc gcactgcttg cagtacacct cggtgcggcg catgccgtat
     1201 gagtcgtcgg ggcgcgtccc cacgttctct gcgaccgccg ggtccgtgaa ggagggccag
     1261 ccggtgccgg aatcgaactt ctgcgcggag gtgaagagga gattcccgca tcccttgcag
     1321 cggtattctc ccgcttcatg gttgttcacg tactcgcccg agaagggcgc ctccgtctcg
     1381 cgctcccgca gcacccggaa ttcctcgggg gagagcttct gttcccattc ttcctccgtt
     1441 cgcggcatct cgtccatata cggatgagta tacgatgaat tgctccggga aggggagagg
     1501 gagtaggatg gagtggatga cgacccgtac ggggcttctc ttcgcgcttc tcgtccttgc
     1561 cgcgctcggt atcatcgatg cctggtacct cacgctctcg gccttccaag gggcagcctt
     1621 ggtctgcgat atcggcgcgg cgctcgatgg ctgcaatatc gtggcgcaga gcccgtattc
     1681 caagctcttc ggcctgccgc ttgcgctcta tggcgtcgcg ttctacggcc tcgtgtttgc
     1741 gctcgtcgcc gcgcttttta tcgctaccca tcatccgttg taccaggcgg cagtcgttct
     1801 cggagtactc gggctcgtgg cctcgcttgg cttcctcttc atccagttcg tgctcatcaa
     1861 ggcgctctgc atttattgcg tcttctcggc cgtcatcgcg gccctgatct gcatcctctc
     1921 cttcgtcctc ttccggaagc acgccccggg gcggcaagcg gcccctatgg tatcctgagg
     1981 ccgtatgaag ggaactgcag tcctcttgac cctcattctc ctcattccgg gcgtggcgct
     2041 cgcctcgacg gtccggaccg atcggacgct cgtcatctcg gagccgatct cggataacgc
     2101 gtacctcgct gggaccgacg tgaacgtctc ggccccgctc ggcggcgacc tcctctccat
     2161 gggcgggacc ctcaccatcg cggcgccggt cgcaggcgac gccttcctcg cgggcggcac
     2221 catcgacgtg aagcgacccg tgcagggaga cgtgcgcgcg gcgggcggac gcatcctcat
     2281 cgaggatgac gtcgcggggg acctcgtgct cgcgggcggc tcggtggaag tcaccggcac
     2341 tgcctcctcg acccgggtcg cgggcggcaa cgtgcgcatc acgggcgggg cgaccggtcc
     2401 tgccgtcgtg tatggcggcg cggtgtacct cgcgggcgaa ttcctcgggg acgtggaggt
     2461 gaccgcatca gaccggctca tgcttgccga gggtacccgc atccatggga cgcttcgata
     2521 caacaccccg cagcaggcgg gcatcccggc ctccgcgatc atcgatgggg gcgtcgagta
     2581 cacgggcggc tcttcctacc tcccgacggt ggaggaggcg aagcgcttcg cggtcgccgg
     2641 cgcaggcgtc ttcttcgtcg cgagcgtgat cgccgcggtc atcgccgctg ggctcctcgc
     2701 cgggcttttc ccaggcctct ccgagcgcgt cacggagcgt acgctcgctc cttccccgag
     2761 gcgtttcgtg ctgcataccc tcctcgggtt tgcgattgtg gtcgcggctc cggttttgat
     2821 tctcttcctt ctgctttcgt tcgtcgggac gctggtcgcg ctcatgctcg ccctggcgta
     2881 cgtcttcctc ctcattctcg ggtatctgta tgccggcatc atcgcgggtg cggcgctcgc
     2941 gcgcggcctc ttcaagcgct catcggtgtc ctggcgcttc gcggtgctcg gcatgttcgt
     3001 gctctatgtc atcggggtga tcccggtctt cgggaagctc gtcatcctcg tgctctcgtc
     3061 cgcggccatc ggcgcgatcg cgatcatcgc gtaccgcttc gcgttcccgc gtgcggacga
     3121 tatcgagcta tgatgcccgc ggcaggggag gtagctccgg agttcgacct tccggaccag
     3181 gaggggagga agcattccct ggccgactac cgcggccggt acgtcctcct ctatttctat
     3241 cccaaggacg acacccctgg ctgcaccaag gaggcctgcg ccatccggga cgcggacccc
     3301 gacttctctg cgctcgacgc ggtcgtgctc ggggtttcgg cggactcggt gaagagccat
     3361 aagaagtttg cgcagaagta cggcctcacg ttcccgctct tggcggacga ggacaggacg
     3421 gtcgtgaacg catacggcgt ctggggccgg aagaagttca tgggccgcga gtacgacggc
     3481 atcttccgta cctccttcct catcgatccg gcagggaaga tcgcgaaggt gtacgagggc
     3541 gtgaagcccg acgcgcatgc cgcgcaggtg ctcgccgacc tgcgcgcgct gcaggtctag
     3601 atatggcgag cacgtttgcg gagcgaaagg agcgcctcgg ccgcatcctc cggtacctca
     3661 agcaggaata tccggtcccc aaatccgagc tcacctacga gaccccgttc cagttcct
//
LOCUS       DASPKG010000064         3335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_234760, whole genome shotgun sequence.
ACCESSION   DASPKG010000064 DASPKG010000000
VERSION     DASPKG010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234760"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VEA36_02735"
     CDS             <1..1004
                     /locus_tag="VEA36_02735"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HYF29256.1"
                     /translation="DLVTFTLLDVSYVNLFFIAYLAIAATCTFGSHILFARRHEEQQN
                     WMRWLAVLFTLGGQYAIGGILSGCLIFYTKSAVLAVSWPFLILLALVFIGNEYFRKYR
                     EHLIFQTVLFFFALYAYAIFALPIYIGSIGPLVFAGSTIAAIVLFGGFLYLLYRTGAA
                     RLSESIKPILASAAIIVVALVASYATGVVPPLPLALKEGGIYHGLTRQGGDYVLKAEA
                     ERPWWNILPQTVHVPPGGTIYAYGAVFAPIRFSTSVVHHWQRYDERSGKWVEEGHVAF
                     PISGGRAEGYRGYSIKENPEPGKWRVSVETLQGQTIGRISFAVERASTLPPLHDEVR"
     gene            complement(1033..1536)
                     /locus_tag="VEA36_02740"
     CDS             complement(1033..1536)
                     /locus_tag="VEA36_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYF29257.1"
                     /translation="MKKRSIFDRLAGTVPLDDYDYDDELPARAPARQAAPQPMMRPAA
                     APQPLQHHIVEEETEGQLPVDVYQTPNEIVIRTFVAGVRPDDLNVSISRDMVVIEGSR
                     DERDQLPDSDYFTRELFWGSFSRTILLPQEIDVDMASAGAKDGLLSIILPKLDKTRQT
                     KLKVRAA"
     gene            complement(1541..2299)
                     /locus_tag="VEA36_02745"
     CDS             complement(1541..2299)
                     /locus_tag="VEA36_02745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HYF29258.1"
                     /translation="MPTLEVVGYAFVGGILPALVWLFFLVKEDARCPEPRSLILLAMF
                     AGMLAVPIVLPLEHYVKAIMPTLTAIPTLFDRFALNLAIEFPVILAWAFIEETVKFVL
                     AALLILWRKQVDEPIDLIIYMICVALGFAALENALFLIAPLANGDVAQTVLANNLRFI
                     GATLLHVIASASVGFAMALAYKEKRHVRPLAVALGLILAIALHALFNFLIIRETASYT
                     VLAFLLVWVGAVVGFGLFELLRYARYNRLPKNTC"
     gene            complement(2286..>3335)
                     /locus_tag="VEA36_02750"
     CDS             complement(2286..>3335)
                     /locus_tag="VEA36_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYF29259.1"
                     /translation="SEIPGIQGGSQVTLKELLRAAVANGGVRIIPGHFEKTYFHWIDN
                     LRPWCISRQIWYGHRVPVWYRGEEVAVGEAPLGEGWVQDEDTLDTWFSSGSWTFSTLG
                     WPDQTDDLARFHPTTLLETGYDILFFWIARMILMSGFALGQVPFKDTYLHGIVRDAQG
                     RKFSKSLGNGIDPIDMIEKFGADAVRMSLLVGNTPGTDMRLSEDKIKGYKHFANKLWN
                     ISRFVLENTEVDSPDAPVTDEDAALLDALSRLAAEVTTDLEEYRLYMAAEKLYHYVWH
                     ELADVILESSKPLLAGGDEVSRRSRAAALRRILREVLILLHPFMPYVTEEIWQSLPEK
                     SADLLMVASWPHADA"
BASE COUNT          616 a         1023 c         1089 g          607 t
ORIGIN      
        1 cggacctcgt cacgttcacg ctcctcgacg tctcgtacgt gaaccttttc ttcattgcgt
       61 acctcgcgat cgccgcgacc tgcaccttcg ggagccacat cctgttcgca cgccggcacg
      121 aagagcagca gaactggatg cgctggctcg cggtcctctt cacgctcggt gggcagtacg
      181 cgatcggcgg catcctctcc ggctgcctca tcttctacac gaagagcgcg gtgctcgccg
      241 tctcctggcc gttcctcatc ctgctggcgc tcgtgttcat cgggaacgag tacttccgca
      301 agtaccgcga gcatctcatt ttccagacgg tattgttctt cttcgcgctc tacgcctacg
      361 cgatcttcgc gctcccgatc tacatcggct cgatcggccc cctcgtcttc gcgggcagta
      421 cgattgcagc gatcgtcctc ttcggcgggt tcctgtatct gctctaccgc acgggtgccg
      481 cgcggctctc ggagagcatc aagcccatcc tcgcctctgc ggcgatcatc gtggtcgcgc
      541 tcgtcgcttc ctacgcgacc ggcgtcgtgc cgccgctccc gcttgcgctc aaggagggcg
      601 gcatatacca cggcctcacg cgccagggcg gcgactacgt cctcaaggcg gaagcggagc
      661 gtccttggtg gaacatcctc ccgcagacgg tgcatgtgcc cccaggcggc accatctacg
      721 cctatggtgc ggtcttcgca cccatccgct tctcgacgtc ggtggtgcac cactggcagc
      781 ggtacgatga gcgctcaggg aaatgggtcg aggaggggca cgtcgccttc ccgatctccg
      841 gagggcgggc cgaggggtac cgcggatatt cgataaagga gaatcccgag cctgggaaat
      901 ggcgggtatc ggtcgagacc ctgcagggcc agacgatcgg gcggatatcg tttgcggtcg
      961 agcgggcaag cacgctcccg ccgcttcacg acgaggtgcg ctaacgaaaa accgcccgaa
     1021 ggcggttctt cgttaggccg cgcggacctt gagcttggtc tgccgcgtct tgtcgagctt
     1081 ggggagaata atcgacagga gaccgtcctt agcgcctgcg gaggccatgt ccacgtcaat
     1141 ttcctggggc aggaggatgg tgcgggagaa ggagccccag aagagctccc gggtgaagta
     1201 atcggaatcg gggagctggt cgcgctcatc gcgggatccc tcgatcacca ccatgtcgcg
     1261 gcttatggaa acgttgaggt cgtcggggcg cacgcccgcg acgaaggtgc ggatgacgat
     1321 ctcgttcggg gtctggtaca catcgaccgg gagctggccc tcagtctcct cctcaacgat
     1381 gtggtgttgc aggggctgcg gggcggccgc cgggcgcatc atcggctggg gcgccgcctg
     1441 ccgagcggga gcgcgcgcgg gaagctcgtc gtcgtagtcg tagtcatcga gcggtacggt
     1501 gcccgcgagt cgatcgaaga tggagcgctt tttcatacga ttagcaggta ttttttggta
     1561 aacggttgta gcgtgcgtac cgcagtagtt cgaaaagtcc gaacccgacc acggcaccga
     1621 cccagacgag gaggaatgcc agcacggtgt acgacgcggt ctccctgatt ataaggaagt
     1681 tgaaaagcgc gtgcaatgcg atcgcaagga taagtccgag cgcgaccgca agcgggcgca
     1741 cgtggcgctt ctccttgtac gcgagcgcca tcgcgaatcc gactgacgcc gatgctatca
     1801 catggaggag tgttgcgccg atgaagcgca ggttgttcgc caagaccgtc tgcgcgacgt
     1861 cgccattggc caagggcgcg atgaggaaga gcgcgttctc gagcgcggcg aacccgagcg
     1921 caacgcagat catgtagatg atgaggtcga tgggctcgtc cacctgcttt cgccagagga
     1981 tgaggagcgc ggcgagcacg aacttcacgg tctcctcgat gaaggcccac gcgaggatga
     2041 cggggaactc gatcgcgagg ttgagggcga agcgatcaaa gagggtcggg atcgcggtca
     2101 gggtcggcat gatggccttc acgtagtgct cgagcggcaa gacgatgggc accgcgagca
     2161 tgcccgcaaa catcgcgagc aggatcaagg agcgcggctc cgggcagcgc gcatcctcct
     2221 tcaccaggaa aaagagccac accagggcag ggaggatccc gccgacgaac gcgtacccga
     2281 ctacttcaag cgtcggcatg gggccaggag gctaccataa ggaggtcagc ggacttttcg
     2341 ggcaggctct gccatatctc ctccgtgacg tagggcatga agggatggag gaggatgagg
     2401 acctcgcgga ggatgcggcg gagcgccgcg gcgcgcgagc gcctcgacac ttcgtcaccg
     2461 cctgcaagca ggggcttcga tgattcgagg atgacgtccg caagctcatg ccacacataa
     2521 tgatacagct tctcggcggc catgtagaga cggtattcct cgaggtcggt ggtcacttcg
     2581 gcggcgagcc tcgagagcgc gtcgaggagc gccgcgtcct catcggttac gggcgcgtcc
     2641 ggtgaatcca cctcggtgtt ctcgaggacg aaccgcgaga tgttccagag cttgttcgcg
     2701 aaatgcttgt accccttgat cttgtcctcg gagagccgca tatcggttcc gggcgtattg
     2761 ccgaccaaga ggctcatgcg caccgcgtcc gcgccgaact tctcgatcat atcgatcggg
     2821 tcgatgccgt tgccgagcga tttcgagaac ttgcgcccct gcgcgtcccg cacgatgccg
     2881 tggaggtagg tatccttgaa cggtacctgc ccgagggcga agccggacat gaggatcatg
     2941 cgcgcgatcc agaagaagag gatgtcgtac ccggtctcaa ggagggtcgt gggatggaag
     3001 cgggcgaggt catccgtctg gtccggccag ccaagcgtgg agaaggtcca ggaacccgag
     3061 gagaaccagg tgtcgagcgt atcctcgtcc tgaacccagc cctcgccaag aggcgcctcg
     3121 ccgaccgcaa cctcctcgcc ccggtaccag accggcaccc ggtgcccgta ccagatctgc
     3181 cggctgatgc accacgggcg caggttatcg atccagtgga aatacgtctt ctcgaagtgc
     3241 ccggggatga tgcggacccc gccgttcgcg accgcagcgc gcaaaagctc cttcagcgtt
     3301 acctgactcc cgccttggat gcccgggatc tccga
//
LOCUS       DASPKG010000065         3841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_507781, whole genome shotgun sequence.
ACCESSION   DASPKG010000065 DASPKG010000000
VERSION     DASPKG010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507781"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1273
                     /locus_tag="VEA36_02755"
     CDS             92..1273
                     /locus_tag="VEA36_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29260.1"
                     /translation="MMPPPPPPVMPLRPEPSHRPRRFPVGTLVIALLVIAASVGALFF
                     FSGAKVKITPTANPITVSGTFAATKSQGELPFELVTVEKTATASVPAESTENVTQSAQ
                     GTIIVQNMQDAPQALIKNTRFETPEGLIFRIRDSITVPAARNGEPGTLSVTVYADEAG
                     ERYNVGPTTFTLPGLSGSDTFSKVNARSMEAMKGGFSGPRPTISEATKDAEFAKLQST
                     LDAELRAAIEGQVREGYVLVPGGTASQFEEQPDTAGAGNTVDLAMRGVIRGVIFPEEM
                     LARTLAYQSVGTYSGQPLKFESLSGLMLAPAEGLIPTEGEESFSFTLTGTTTLVWKVD
                     PAKVSAAVAGKSREQAEVALQALPEVDGALLVLKPFWVGSFPADPAKITVSVEGEEEA
                     K"
     gene            1380..1598
                     /gene="infA"
                     /locus_tag="VEA36_02760"
     CDS             1380..1598
                     /gene="infA"
                     /locus_tag="VEA36_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013352.4"
                     /note="stimulates the activities of the other two
                     initiation factors, IF-2 and IF-3; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HYF29261.1"
                     /translation="MADSKEGDAVTGTVEEVLPNSLFRVRMPSGDLQLAYLAGKMRLH
                     RIRVLVGDSVELVLDPYGGRARIVRRLT"
     gene            1605..1721
                     /locus_tag="VEA36_02765"
     CDS             1605..1721
                     /locus_tag="VEA36_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HYF29262.1"
                     /translation="MKVRASVKVQQPGDIIVRRRGRLYRINKRNPRRKARQG"
     gene            1740..2120
                     /gene="rpsM"
                     /locus_tag="VEA36_02770"
     CDS             1740..2120
                     /gene="rpsM"
                     /locus_tag="VEA36_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HYF29263.1"
                     /translation="MRIAGVTLPDDKQLLYALPLIYGIGFTRARSILKEAGIAENAKG
                     KDLSAEEEQKIRSLAEAFTIEGELRREVGQNVKRLKDIRSARGMRHERRLPVKNQRTK
                     TNSRTVRGNTRKTATSGKRKLEKT"
     gene            2183..2578
                     /gene="rpsK"
                     /locus_tag="VEA36_02775"
     CDS             2183..2578
                     /gene="rpsK"
                     /locus_tag="VEA36_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HYF29264.1"
                     /translation="MDQGRKERALSKGSKKRLERGVLHVEATFNNTKAVLTDEKDNAV
                     MASSAGALGFSGARKSTPYAAAKVGELLGEKAQAIGLKEASVVIRGIGAGRESALRAF
                     AGKGIAIRAIKDATPVPHNGPRPPKPRRV"
     gene            2588..3208
                     /gene="rpsD"
                     /locus_tag="VEA36_02780"
     CDS             2588..3208
                     /gene="rpsD"
                     /locus_tag="VEA36_02780"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HYF29265.1"
                     /translation="MKIGPKYKIAKRLGASVFPKTQTQKFAISEERALMNKKRGGMRR
                     GATEFGKQLLEKQKVRMTYGISEKQFSNYVAEAMGTRGVNPADMLHRRLELRLDNVVY
                     RLGLAPSRRAARQMVSHGHITVNGVKTTIPSRAMRPGDKIAVREGSRKNVPFEGLAER
                     FNERPKLASWLSFDPKSMQGGVEEVPTQDSADTAGDLVAVLSFYSR"
     gene            3242..>3841
                     /locus_tag="VEA36_02785"
     CDS             3242..>3841
                     /locus_tag="VEA36_02785"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HYF29266.1"
                     /translation="MEYHITLPSKPRVVKEEELQGIYEIDSLYPGYGHTLGNSLRRII
                     LSSLPGAAVTAVKIEGVSHEFATIEGVRESVMEVLLNLKRVHFSLQGDEPQTISLSVK
                     GPKQVTAADFNLPSQVQIANPDQHIADISGKIAFELEATIERGLGYVPRETLTKEKVD
                     IGTIALDATFTPIRRVNYEVEDMRVGDRTNFNRLRILIET"
BASE COUNT          723 a         1237 c         1213 g          668 t
ORIGIN      
        1 ggaagcgggt tctaccccgg tgcgcatgac cccggatgcg gacgttccgc cgcccccacc
       61 gtccgcgcct ccttcagcag agccaccgcg catgatgcct ccgcccccgc ctccggtcat
      121 gccgctccgg cctgagccgt cgcatcgccc gcgccgcttc ccggtcggga ccttggtcat
      181 cgcgctcctc gtgatcgccg catcagtggg cgccctcttc ttcttctcgg gcgccaaggt
      241 gaagatcacc ccgaccgcga accccatcac ggtctcgggc accttcgcgg cgacgaagag
      301 ccagggcgag cttccgttcg agctcgtgac ggtcgagaag accgccaccg cctccgttcc
      361 ggcagagtcc accgagaacg tcacccagtc cgcgcagggg acgatcatcg tgcagaacat
      421 gcaggacgcg ccccaggccc tcatcaagaa cacgcgcttt gagacccccg agggcctcat
      481 cttccgcatc cgcgattcca tcaccgtccc ggctgcgcgt aacggcgaac cgggcacgct
      541 ttccgtcacg gtgtacgcgg acgaggcagg ggagcggtac aacgtggggc caaccacttt
      601 cactctcccg gggctttccg gctctgatac cttctcgaag gtgaacgccc gttcgatgga
      661 agccatgaag ggcggtttct cgggtccccg gccgaccatc tcggaagcca cgaaggacgc
      721 tgaattcgcg aagctccaga gcaccttaga tgccgagctg cgtgcagcca tcgagggcca
      781 ggtgcgcgaa ggatatgtct tggtccctgg cggcaccgca tcgcagttcg aggagcagcc
      841 ggataccgca ggggcaggga acaccgtcga tctcgcgatg cgcggcgtca tccgcggggt
      901 gatcttcccc gaggagatgc tcgcccgcac ccttgcgtac cagtctgtgg ggacgtattc
      961 cgggcagccc ctgaagttcg agtcgctgtc gggcctcatg ctcgcgcccg cggaaggcct
     1021 catcccgacc gagggcgagg agagcttctc cttcacgctg accggcacta ccaccctcgt
     1081 gtggaaggtg gacccggcca aggtatccgc tgcggtcgcg ggcaagtccc gcgaacaggc
     1141 cgaagtggcg ctccaggcgc ttccggaagt ggacggggcg ctccttgtgc tcaagccctt
     1201 ctgggtcggc tccttcccgg cagatcccgc gaagatcacg gtctcggtgg agggcgagga
     1261 ggaggcgaag tagcgagcgt ttgacgaact aaggtagaag gcctgccccg gcgggtcttc
     1321 cctcttccgg gctgcatttg acggtttatt cactaattgt taagataccc aggcttcaca
     1381 tggcggatag caaagaggga gatgccgtaa ctggcaccgt tgaagaggtg cttcctaact
     1441 cgttatttcg agtgaggatg ccttcgggag acctccagct tgcctatctc gccggcaaga
     1501 tgcgcttgca ccgtatccgc gtgctggtgg gggactcagt cgaactggtc ctcgatccgt
     1561 acggggggcg ggcgcgcatc gtgaggcgcc ttacctaacc ctcgatgaaa gtccgcgcat
     1621 ccgtaaaggt ccagcagcct ggagatatca tcgtccgccg ccgcgggcgc ttgtatcgca
     1681 tcaacaagcg caatccccgc cgtaaggcgc gtcagggcta attttcgtac ttcattccta
     1741 tgcgtatcgc cggcgttacc cttcctgatg acaagcagct cctctacgcg ctgcccctca
     1801 tctatggcat cggcttcacc cgtgcccgca gcatcctcaa ggaggccggc attgcggaga
     1861 acgccaaggg caaggacctc tccgctgagg aggagcagaa gatccgctcg ctcgcggaag
     1921 ccttcaccat cgagggcgag ctccgccgtg aggtgggcca gaacgtgaag cgcctcaagg
     1981 acatccgttc cgcccgcggc atgcgccacg agcgccggct cccggtcaag aaccagcgca
     2041 cgaagaccaa ttcgcgcacg gtccggggca acacccgtaa gaccgcgacg agcggcaagc
     2101 gcaagctcga gaagacctaa tcctgtcgct tcattaattt cctatgggta agaaacgtat
     2161 catcaagaag tcgggcaggg gcatggacca gggccgcaag gagcgggcgc tgtccaaggg
     2221 ctcgaagaag cgtctcgagc gcggggtgct ccacgtggag gccaccttca acaacaccaa
     2281 ggccgtcctc accgatgaga aggataacgc cgtcatggcg agctcggcgg gtgccttggg
     2341 cttctcgggc gcccgcaagt caacgcccta cgccgccgcg aaggtgggcg agctgcttgg
     2401 cgagaaggcg caggccatcg gcctcaagga ggcatcggtc gtcatccgcg gcatcggcgc
     2461 cggccgcgag agcgcgctcc gcgcgtttgc cggcaagggc atcgcgatcc gcgccatcaa
     2521 ggacgccacc ccggtgccgc acaacggccc gcgtccgcct aagccgcgtc gcgtctaatc
     2581 ctgtcgtatg aaaatcggac ctaagtacaa gatcgccaag cgcctcgggg catccgtgtt
     2641 cccgaagacg cagacgcaga agttcgcgat ctccgaggag cgcgccttga tgaacaagaa
     2701 gcgcggcggt atgcgccggg gcgcgaccga gttcggcaag cagcttctcg agaagcagaa
     2761 ggtgcgcatg acctacggca tctcggagaa gcagttttcc aattacgttg ctgaggccat
     2821 gggcacccgc ggagtgaacc cggcggacat gctccaccgc cgcctcgagc tccggctcga
     2881 taacgtggtg tatcgcctgg ggctcgcgcc gtcgcgccgc gcggcgcgcc agatggtctc
     2941 gcacgggcac atcacggtga acggggtgaa gaccaccatc ccgtcgcggg cgatgcgccc
     3001 gggcgataag atcgccgttc gtgagggttc gaggaagaac gtgccgttcg agggccttgc
     3061 cgagcgcttc aatgagcgcc cgaagctcgc gtcctggctc tcgttcgacc cgaagagcat
     3121 gcagggcggg gtggaggagg tgccgaccca ggactccgcg gataccgcag gggacctggt
     3181 ggccgtgctc tcgttctaca gccgctagtt tttattaatc cactggttaa ctgattgagt
     3241 tatggaatac cacattacgc ttccgtcgaa gccccgcgtc gttaaggaag aggagcttca
     3301 gggcatttat gagattgatt cgctctaccc gggatacggg catacgctcg gcaattcgct
     3361 tcgccgtatt attctttcct cgcttccggg cgcggcggtg accgcggtca agatcgaggg
     3421 ggtgtcgcat gaattcgcga cgattgaagg cgttcgcgag tcggttatgg aggtgctcct
     3481 gaaccttaag cgcgtgcact tctcgctcca gggagatgag ccgcagacca tatcgctatc
     3541 cgtgaaggga ccgaagcagg tcacggcggc ggatttcaac ctgccgagcc aggtgcagat
     3601 cgcaaatccg gaccagcata tcgcggacat ctcggggaag atcgccttcg agcttgaggc
     3661 gacaatcgag cgggggctcg gctacgttcc ccgcgagacc ctcaccaagg agaaggtgga
     3721 catcgggacg atcgcgcttg acgcgacctt caccccgatc cgccgtgtga actacgaggt
     3781 ggaggacatg cgcgtcggcg accggacgaa cttcaatcgg ctccgcatcc tcatcgagac
     3841 c
//
LOCUS       DASPKG010000066         4087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_373147, whole genome shotgun sequence.
ACCESSION   DASPKG010000066 DASPKG010000000
VERSION     DASPKG010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373147"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..127
                     /locus_tag="VEA36_02790"
                     /pseudo
     CDS             1..127
                     /locus_tag="VEA36_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019795518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
     gene            198..884
                     /locus_tag="VEA36_02795"
     CDS             198..884
                     /locus_tag="VEA36_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYF29267.1"
                     /translation="MEQAPTGSFWEKYLTPIAVLLGALIIAGALAYGRGDAGPSAQTG
                     NQPVKTVDIKDVKTSGSPSIGNANAPATLAVWFDYQCGYCKRFDAETLSALKASHVDT
                     GKLRIVFKDFQFFPGSEDVGVFGRAVYEAYPDRHYAWFSGMMALPAGESGLTVAKAKE
                     VATAVGLDAARIEQLATQNRAKYLAAVQADKAEGTAFGITGTPASIVGKQLLEGAQPL
                     AAVTAAVEAK"
     gene            959..1102
                     /locus_tag="VEA36_02800"
     CDS             959..1102
                     /locus_tag="VEA36_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29268.1"
                     /translation="MHIEWNRVTWYSQVVAIMLFFAIFALGFYLGQKHNTDVCEYVRE
                     IGR"
     gene            1099..1506
                     /locus_tag="VEA36_02805"
     CDS             1099..1506
                     /locus_tag="VEA36_02805"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HYF29269.1"
                     /translation="MSEEEFRALAEEEWTKVPDRFREEVKNVALLIEDEPSEEVRKQE
                     GLEEGETLLGLYHGIPNTERGSEYGVGVTMPDTITLYRLPLIEEAEHMDREFRDAMRI
                     AIRETIWHELAHYFGHPEEPINEREGEGTNRFE"
     gene            1574..2782
                     /locus_tag="VEA36_02810"
     CDS             1574..2782
                     /locus_tag="VEA36_02810"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HYF29270.1"
                     /translation="MRTFISFVLAAILAALIPFSASAAAPVCDFTRDLEVGVDGEDVH
                     CLQKYLNDAGITIASEGVGSPGNETSLFREKTKEAVVKWQAKNGLPASGFFGALSRAK
                     YKALTGATPATPATPATPGVTPATPATPATPALQSQIAALLAQIEKLKQNPATPTVSN
                     KAVQDQIRKAINAIEDADEQLGDAGGGSEAAKANFNEARDDLFKAVDAYFDGNMTKAQ
                     EYADDAYDNAQDAFEEAGGVSSEDEAEDLIEKVEDRIEEARDTIEDADDDGKDVDEAE
                     ELLDDAERKLEEAQNAFDDEEYEEAEELADDADELVDDALDAIGGSKEDDAEDAIRDA
                     KEAIADAKEAIEEADDDGEDTDDAEELLEEAEDKLDEAEEAFDDEDYDDAEELANEAK
                     ELAGDAEDEL"
     gene            complement(2825..3475)
                     /locus_tag="VEA36_02815"
     CDS             complement(2825..3475)
                     /locus_tag="VEA36_02815"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HYF29271.1"
                     /translation="METMFEWINQFLTSLAYVLPLELFVFLGSFIEEIVAPIPSVLIT
                     TLAGSLALAAGYGWWGLLWLAVLASFGKSLGCAILYVIADKAEDFLLVRLGPKIGITH
                     AEVERVGKFFTGGIRDYLILIVIRALPLSPSLPISVGAGIIKIPFAMYNIATFIGNLL
                     RSLLFIAVGYTGFSAYQRLLDEAQSLESLIQIALAVLLAGFVLVIYYLRRVREKRA"
     gene            3593..3982
                     /locus_tag="VEA36_02820"
     CDS             3593..3982
                     /locus_tag="VEA36_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HYF29272.1"
                     /translation="MAKTLAMVFGVVFVLVGILGFVPNPLVGMEAIFETDMLHNVVHI
                     LFGVILIVAAKKSPAASSLWLIILGAVYLVLAVLGFMMVPDEGELLGLVHVNMADHWL
                     HIVLGVALVAAGFLSKKSAAAPMGPTM"
BASE COUNT          848 a         1258 c         1327 g          654 t
ORIGIN      
        1 cctcgacatc tcgggcgtct cgcacgtcat caactacgac ctgccggcct cctacgagga
       61 ctacacccac cgcatcggcc ggaccggccg ggcggggaag atcggcaagg cgctgacctt
      121 catctcgtaa ccgaaaaccc caggcttcac cgcctggggt ttttggtata ctgggcggac
      181 taaacctaag gcactatatg gaacaagcac caaccggttc gttttgggag aagtatctga
      241 cgccgatcgc ggtcctcctc ggcgcgctca tcatcgcggg cgcgctcgcc tacgggcgcg
      301 gcgacgccgg cccctcggcg cagacgggca atcagcccgt gaagacggtg gacatcaagg
      361 acgtcaagac gtccggcagc ccgtccatcg gcaacgcgaa cgcgcccgcg acgctcgcgg
      421 tctggttcga ttaccagtgc gggtactgca agcgcttcga tgccgagacc ctctcggcgc
      481 tcaaggcgag ccacgtggat accggcaagc tgcgcatcgt gttcaaggac ttccagttct
      541 tcccgggctc ggaggacgtc ggcgtcttcg gccgcgcggt ctacgaagcg tatccggacc
      601 ggcactacgc atggttctcc ggcatgatgg cgctcccagc cggcgagagc gggctcacgg
      661 tcgccaaggc gaaggaggtc gcaaccgcag tcggcctcga tgccgcccgc atcgagcagc
      721 tcgccacgca gaaccgcgca aaatacctcg cggcggtcca ggcggacaag gccgagggca
      781 cggccttcgg gatcacgggc acccccgcat ccatcgtcgg gaagcagctc ctcgagggcg
      841 cccagcccct tgcggcggtc acggctgcgg tcgaggcgaa gtaagcatgt gcgcaaaaga
      901 caaccggccc tcctcgaggg ccggttgtct tttggtacgg ccgggctatc ctttccccat
      961 gcatatcgaa tggaacaggg tcacctggta ctcccaggtc gtcgcgatca tgctcttctt
     1021 cgcgatcttc gcgctcggct tctatctcgg ccagaagcac aatacggacg tttgcgaata
     1081 cgttcgtgaa atagggcgat gagcgaggag gaatttaggg cgcttgcgga ggaggaatgg
     1141 accaaggttc cggaccgctt ccgcgaggag gtgaagaacg tcgcgctctt gatcgaggat
     1201 gagccgagcg aggaggtccg taagcaggaa gggctcgagg agggcgagac cctgcttggc
     1261 ctctaccacg gcatcccgaa tacagaacgc gggagtgagt acggggtcgg ggtgacgatg
     1321 cccgacacga tcaccctgta tcgtcttccc ttgatcgagg aggccgagca catggaccgg
     1381 gagttccggg acgccatgcg gatcgcgatc cgggagacga tctggcacga gctcgcgcac
     1441 tatttcgggc atcccgagga gcccatcaac gagcgggagg gggaagggac gaatcggttt
     1501 gaataacgcg gttgtagctc gcgcctcgag cgactatcat agaagcatta gattactggc
     1561 cttacccgtc cctatgcgta ccttcatttc attcgtgctc gccgccattc ttgcggccct
     1621 tatcccgttc tccgcatccg ccgcagcccc cgtctgcgat ttcacccgag acctcgaggt
     1681 cggggtggac ggcgaggacg tccactgcct ccagaaatac ttgaacgatg ccggcatcac
     1741 catcgccagc gagggcgtcg gttctcccgg caatgagacg agcctcttcc gcgagaagac
     1801 taaggaggcg gtcgtgaagt ggcaagcaaa gaacgggctt ccggcgagcg gcttcttcgg
     1861 cgcgctctcc cgcgccaagt acaaggcgtt gactggcgcg acgcccgcca cgccagcaac
     1921 ccccgccact ccgggagtaa ccccagcaac gcctgcgacg cccgcaaccc cggcgctcca
     1981 gtcgcagatc gcagcgcttc tcgcgcagat cgagaagctc aagcagaacc cggcaacccc
     2041 gaccgtctcc aataaggccg tccaggacca gatccggaag gccatcaacg cgatcgagga
     2101 cgcggatgag cagctcggcg acgccggcgg cggctcggaa gcggcaaagg ccaacttcaa
     2161 cgaagcgcgc gacgacctct tcaaggcggt cgatgcttac ttcgacggca acatgacgaa
     2221 ggcgcaggag tacgccgacg acgcctacga caacgcgcag gacgccttcg aggaggcggg
     2281 cggcgtctcc agcgaggacg aggccgagga cctcatcgag aaggtggagg atcgcatcga
     2341 agaagcgcgg gacaccatcg aggatgcgga cgacgatggc aaggacgtgg acgaggccga
     2401 ggagctcctc gatgacgccg agcgcaagct cgaggaagcg cagaacgcct tcgatgacga
     2461 agagtatgag gaggccgagg aattggcgga tgacgccgac gagctcgtgg acgatgcgct
     2521 cgatgccatc ggcggaagca aggaggacga cgccgaggac gccatcaggg acgcgaagga
     2581 ggcgatcgcg gacgcgaagg aggccatcga agaggcggac gatgacggcg aagataccga
     2641 tgacgccgag gagctcctcg aggaggcgga agacaagctc gacgaggccg aggaggcgtt
     2701 cgatgacgag gactacgacg atgccgagga gctcgcgaac gaagccaagg agctcgccgg
     2761 ggacgccgaa gacgaactct aggaatacga acgcagaaac ggccggggac tcccggccgt
     2821 ttcgctatgc ccgcttctcg cggacgcgcc ggagatagta gatgacgagg acgaagccgg
     2881 cgaggagtac cgcaagcgcg atctggatca gggactcgag cgactgggcc tcgtcgagga
     2941 ggcgctggta cgccgagaag cccgtgtacc cgaccgcaat gaacagcagg ctccggagca
     3001 gattcccgat gaaggtcgcg atgttgtaca tcgcaaacgg aatcttgatg atgccggcgc
     3061 cgaccgagat cgggagggag ggggacagtg ggagcgcccg gatgacgatc aggatcaggt
     3121 aatcgcggat gccgcccgtg aagaacttcc cgacccgctc cacctccgca tgcgtaatgc
     3181 cgatcttggg gccgagccgc accaggagga aatcctccgc cttgtccgcg atgacgtaca
     3241 ggatggcgca gccgagcgac ttcccgaagg acgcgagcac cgcaagccac aggagccccc
     3301 accagccgta gccggccgca agcgctagcg accccgccaa ggtcgtgatg aggaccgagg
     3361 ggatgggggc gacgatctcc tcgatgaagg agccgaggaa gacgaagagc tccaagggga
     3421 gcacgtaggc gagcgaggtg aggaattgat tgatccattc gaacatggtc tccatactac
     3481 cccctctcct gcgctatcca cagcgcttcc gggcttaagc cccgcttcat atagcagagc
     3541 tatacttacg gcatcaggtt catcctgatc cctaaccctt ttactccgta ctatggcaaa
     3601 aacgcttgca atggtgttcg gtgtcgtctt cgtgctcgtc ggcatcctgg gcttcgtgcc
     3661 caatccgctc gtcggcatgg aggcgatctt cgagaccgat atgctgcata acgtcgtgca
     3721 catcctcttc ggcgtgatcc tcatcgtcgc tgcgaagaag tcgccggcag cctcgtcgct
     3781 ctggctcatc atccttggtg ccgtctacct cgtgctcgcg gtcctcggct tcatgatggt
     3841 gccggacgag ggcgagctgc tcggcctcgt gcacgtgaac atggccgatc actggctcca
     3901 tatcgtgctc ggcgtggcgc tcgtcgccgc tggcttcctc tcgaagaaga gcgctgcggc
     3961 accgatgggt cctacgatgt aagcagtggg tttgccgaaa gcgcgtcgca tgcgcggcgc
     4021 gctttcgcat tacgtaaata agaccagaca aaatgtctgg cctacgggtg tttggggggt
     4081 gaagcgg
//
LOCUS       DASPKG010000067         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_510677, whole genome shotgun sequence.
ACCESSION   DASPKG010000067 DASPKG010000000
VERSION     DASPKG010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510677"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VEA36_02825"
     CDS             <1..1263
                     /locus_tag="VEA36_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYF29273.1"
                     /translation="IHVDEYQDTNALQGRLAGYLARAHGNIFVVGDIDQTIYTWRGAT
                     IENLLAFEETYPSARTIVLAHNYRSTKTIVEAANQIIEKNQNRKEKISVTDNPDGEPI
                     RLYIADTAETEARYVARQARSEIQSGRAPGEIAVLYRTNFQSRAIEEAFLHAGVPYKL
                     LGTRFLDRKEVKDVLAWVRLALEPTREADRMRAVQAPARGIGKATLGKLAAGRREELK
                     PAERTKVEAFEALVAELNREAKVMLPSAFVRAVIERSGMALTLEKGGEDDQERLENVK
                     ELATLATRYDSMPGEEGIAAFLADTALAGDQDELDRRPQEKGVTLMTVHAAKGLEFES
                     VYVTGMEEGLFPHEGMGDGERDQEEERRLFYVAVTRAKKRLTLTLARIRRLYGTDYFS
                     EPSHFLYDIDEGLIYSVPPDGEGEVIID"
     gene            1263..2045
                     /gene="rsmA"
                     /locus_tag="VEA36_02830"
     CDS             1263..2045
                     /gene="rsmA"
                     /locus_tag="VEA36_02830"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HYF29274.1"
                     /translation="MAFAKKSLGQNFLMHAQTAERIVAAANVPTGATVLEIGPGTGML
                     TRALLDAGLWVIAVEADGALLPTLEEAFTSEIGEGRLRLVHADIRAFDASNLPAQYHV
                     VANIPYYITGELIRMFLSGAHKPRSMTLLVQKEVAERITRDPKGSLLSIAVKAYGTPI
                     YRFTVPRGAFKPAPTVDSAVLHIQGISGDSFASLEEEAWFFEVLRTGFGHKRKQLAKN
                     LEALAPRHAVLDALRACSRSEKVRAEDLSLHDWIALARSLRA"
BASE COUNT          415 a          657 c          731 g          356 t
ORIGIN      
        1 atccacgtgg acgagtacca ggacaccaat gcgctccagg gaaggcttgc ggggtatctc
       61 gcgcgcgcgc acggcaacat cttcgtggtg ggcgacatcg accagaccat ctacacctgg
      121 cgcggcgcaa ccatcgagaa cctgctcgcc ttcgaggaga cgtatccgtc ggcacggacc
      181 atcgtgcttg cgcataatta ccgctcgacg aagacgatcg tcgaggcagc gaaccagatc
      241 atcgagaaga accagaaccg caaggagaag atctcggtga cggacaaccc ggacggggag
      301 ccgatccgcc tctatatcgc ggacacggca gagactgaag cgcggtacgt cgcgcggcag
      361 gcacgctcgg aaatccagag cgggcgagcg ccgggcgaga tcgcggtctt gtaccgcacg
      421 aacttccagt cccgcgcgat cgaagaggcg ttcctccatg ccggcgtgcc gtacaagctc
      481 ttagggacgc ggttcctcga ccgcaaggag gtgaaggacg tgctcgcctg ggtgcggctt
      541 gcactcgaac caacgcgcga ggcggatcgc atgcgtgcgg tgcaggcgcc tgcgcgcggg
      601 atcggcaaag cgacgcttgg gaagcttgcg gcagggaggc gcgaggaatt gaagcctgcc
      661 gagcgtacga aggtcgaggc gttcgaggcg cttgtcgcgg aactcaaccg ggaggcgaag
      721 gtgatgctgc cgtccgcgtt cgtgcgcgcc gtgatcgagc gaagcggcat ggcgctcacg
      781 ctcgagaagg gcggggagga cgaccaggag cggctcgaga acgtgaagga gctcgcgacc
      841 ctggcgacgc gttacgacag catgccgggc gaggagggca tcgcggcctt ccttgcggac
      901 accgcgcttg ccggcgacca ggacgagctc gatcgcaggc cccaagagaa gggcgtgacc
      961 ctgatgacgg tgcacgcggc gaagggcctc gaattcgaga gcgtctacgt gaccggcatg
     1021 gaggaggggc tcttcccgca cgagggcatg ggcgacgggg agcgggacca ggaagaggag
     1081 cggcgcctct tctatgtcgc ggtgacgcgg gcgaagaagc gcctcacgct gaccctcgcg
     1141 cgcatccgcc gcctctacgg gaccgattat ttctccgagc cctcgcactt tttgtatgac
     1201 atcgatgagg ggctcatata ttcggtaccg ccggacggcg agggggaggt gataatagac
     1261 tgatggcgtt cgcgaagaaa tcgctcgggc agaacttcct catgcacgcg cagacggcgg
     1321 agcgcatcgt cgcggcagca aatgttccga ccggtgcgac cgtgctcgag atcgggcccg
     1381 gtaccgggat gctcacccgc gcgctccttg atgccggact ctgggtgatc gcggtcgagg
     1441 cggacggcgc gctcctgccg acgctcgagg aggcgttcac cagcgagatc ggggaaggga
     1501 ggctccggct cgtgcatgcg gacatccggg cgttcgatgc gtccaacctc ccggcgcagt
     1561 accacgtcgt ggcgaatatc ccgtactaca tcaccggaga attgatccgc atgttcttgt
     1621 ccggggcgca taagccccgc tccatgactc ttcttgtcca gaaggaggtc gcggagcgca
     1681 ttacgcggga ccccaagggg agcctgctct cgatcgcggt gaaggcctac ggcactccta
     1741 tatataggtt cacggtcccg cggggcgcct tcaagcccgc gcccaccgtg gactccgccg
     1801 tcctacatat ccagggcatt tcgggcgatt ccttcgcctc tctcgaggag gaagcctggt
     1861 tcttcgaggt cctgcggacc ggattcgggc ataaacgcaa gcaactagcg aagaatctgg
     1921 aggcccttgc cccgcggcat gcggttctcg acgcgcttcg ggcgtgctcg cgttcagaga
     1981 aagtacgcgc cgaggacctc tcgctccatg actggatcgc gcttgcgaga tcgctgcgcg
     2041 cttaatgcac acatgatgaa aatagcctga aacggcttag tttcgcgtga taggatgagg
     2101 ataccgtggc tcattctacc cgcaccgtcc ccatcatcgt cgcctcggtg gtcgcggtg
//
LOCUS       DASPKG010000068        10338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1998405, whole genome shotgun sequence.
ACCESSION   DASPKG010000068 DASPKG010000000
VERSION     DASPKG010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10338)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10338
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998405"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /gene="secF"
                     /locus_tag="VEA36_02835"
     CDS             <1..612
                     /gene="secF"
                     /locus_tag="VEA36_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HYF29275.1"
                     /translation="TELRFNSIGPSLGRELTTKALYAIGAVLLAIMLYIAFAFRKVSR
                     PVPAWGYGITSVAMLAIDIIVPAGFFAVYAYFTGAQVDTLFVIALLALMGYCINDVIV
                     IFDRVREHLRWNEERNVKEPFEETVGKSITETLGRSINTSLTVALALLAIILVGSEST
                     RIFALVMLVGVVVGTFSSIVRSAPLLIPIATWAEKREAAKKKG"
     gene            624..1175
                     /locus_tag="VEA36_02840"
     CDS             624..1175
                     /locus_tag="VEA36_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYF29276.1"
                     /translation="MLIGITGTNGSGKGTVVEYLVMHKGFSHFSARMFIIEEVVKRGL
                     KQNRRTMREVANEMRKEHSPSYLMERLYEMAKDEPKAVLESVRTIGEAEFLKAKGVYL
                     FAVDADRTLRHERIVARGAEGDNLSYEEFCEQEDAEMASPDPWDMNVFGVMQISDARL
                     MNNGTVEDLHHQVDEALAAVQKV"
     gene            1172..1723
                     /locus_tag="VEA36_02845"
     CDS             1172..1723
                     /locus_tag="VEA36_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYF29277.1"
                     /translation="MRIVGIAGSFGAGKGVVVDYLTQNLGFNHYSASGFITEEIVRRG
                     MPVNRDSMIVVANDLRATNGPSYLIDSLFERAKEGGKDAVIESLRAVAEVRRIKELGG
                     FVLGVDAEPRLRYERAVKRGSEKDAVSYEKWLEQERAESNPDDPTKQNIFGALKEADA
                     IVSNNGSLEELYKQVDEVLAAIT"
     gene            1729..2205
                     /locus_tag="VEA36_02850"
     CDS             1729..2205
                     /locus_tag="VEA36_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYF29278.1"
                     /translation="MPHIHEKIDYAGDVFIVNDGAVLLRMHDKYHMWLPPGGHVELDE
                     DFVEAALREAKEETGLDVKLIGDNGTPTESTGTDIYGERELLIPRFVNRHRINEMHEH
                     ISFIYFGVSDTREVNPAEGEKRDCFKWFTAKELDDPSYGVNERVRKYAKAALDAAA"
     gene            2207..2983
                     /locus_tag="VEA36_02855"
     CDS             2207..2983
                     /locus_tag="VEA36_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HYF29279.1"
                     /translation="MDFAVTVLPVLSGLRDILMPHWGNVEVLKEKGAGAHDIVTELDI
                     AVEERTRTELAELYPDIAFVGEEGGGDRTADRLWLMDPIDGTGHYLRGLPFCTSMLAY
                     IEAGQVRFSAIYDFVNDRVYWAERGKGAYCNGDRLGVSNRKLRESYVAWESNQSEAAN
                     RELTQLLRQETNLFNSINAGWEMAMVASGKLDARICFEPYGKDYDFAPGSLLVEEAGG
                     VVANVGTTTYDYRNTSFIAAGPTLYQELTEGERSIFPITR"
     gene            complement(2973..3674)
                     /gene="pyrH"
                     /locus_tag="VEA36_02860"
     CDS             complement(2973..3674)
                     /gene="pyrH"
                     /locus_tag="VEA36_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HYF29280.1"
                     /translation="MRFIRAKREYVVVSVGGSLIVPDGIDVSFLKRFRELIVSKTRAG
                     LSFFIITGGGRTARNYQGAVAEVRGGLNRDDVDWLGIHSSRLNAHLMRAVFKEEAQAR
                     IVKNPSHRIRAKMPVIIGAGWKPGWSTDYCAVIAAKTLGAKKLVNLSNITHVYTADPK
                     QDPSATPIETIGWAEFRKLIPAEWDPGLSSPFDPVAAREAEKIGLEVAIMNGGNLEEF
                     DKYLSHQPFTGTVIS"
     gene            3823..4221
                     /locus_tag="VEA36_02865"
     CDS             3823..4221
                     /locus_tag="VEA36_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29281.1"
                     /translation="MTTLAEVGESRARLRTRTPRDQPEHLTAVWHEADGVRYARATLS
                     ALGFSTPPLWAEVLDRVRAYGGHCDFADLPRIGEGLIELADAVRPAMPAQGGEIVVCI
                     GDASVGKVRVPIEPARHISLDVEVAYRLAA"
     gene            4443..5810
                     /locus_tag="VEA36_02870"
     rRNA            4443..5810
                     /locus_tag="VEA36_02870"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            6101..6559
                     /locus_tag="VEA36_02875"
     CDS             6101..6559
                     /locus_tag="VEA36_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HYF29282.1"
                     /translation="MGRTLPTVSNDYVVGLTDGEGCFYVNIWKSSAYRAGAGVQLHFH
                     LKMQERDRDLLEAVKNTIGCGAVYFQKEQRTNHCQCYRYTVSATRDILSTVIPFFKQY
                     PLQSATKRASFELFCQIAQLVQAGRHRTIEGLEEIRVLKSRMNQKTVGLA"
     gene            7243..7315
                     /locus_tag="VEA36_02880"
     tRNA            7243..7315
                     /locus_tag="VEA36_02880"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7276..7278,aa:Ile,seq:gat)
     gene            7370..7903
                     /locus_tag="VEA36_02885"
     CDS             7370..7903
                     /locus_tag="VEA36_02885"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HYF29283.1"
                     /translation="MRHSIIVLVLCILAILVGAYLFYTNTQGLGADTLEQSAQVANAP
                     VESMPVAFTVIESGTSASDVTVRKNVAARDQEAYLRLYALARGGQELAPPPVDFNTEY
                     VIGVFAGEKPTGGHMIEVTSIRDLGDTRTLSVTETVPGASCVVTQAFTAPYQLIRVPV
                     SMHYLKAVDTRVASDCL"
     gene            complement(7905..8645)
                     /locus_tag="VEA36_02890"
     CDS             complement(7905..8645)
                     /locus_tag="VEA36_02890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYF29284.1"
                     /translation="MRIFQTEFGHAYGRFYAFGYTSYAELAPEDDPAEAYALGFLPYS
                     TDPSVHNLLYLCRSVRVPLQAWSTTSENRRILKKFDGVFTRTLLSREALQQDPAFLDC
                     FLAYFAGRHTDSSMPRARVLGILESALPLRGVRYDTETGPVAYVLEVVGETFIHYWYS
                     CYDLAYVEKSLGMWLMLDSVRQAKAGGKAYAYLGTAYGDKGKYKMNLEPLEFWDGTKW
                     NANPTLLKQRIKEDAERTVSFPDRLASP"
     gene            complement(8649..10055)
                     /locus_tag="VEA36_02895"
     CDS             complement(8649..10055)
                     /locus_tag="VEA36_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HYF29285.1"
                     /translation="MPEKKSVHFVGICGIGMSGVALLLKEAGWEVTGSDAECYGPPKR
                     ILEKGGLAPRLSYTPDNISPYVDRFVIGRNAKLAPDVNEEVKAAHNTGKPILSFPEVL
                     GELTRERQNIVVAGSYGKSTTTSLIAHILRHSGVDAGYFIGGEPLSLPLPAGLGSHPV
                     FVAEGDEYPSAHDDPRAKFMHLHPRDILLTAVVHDHVNVYPTFESYQRPFTDLLALVP
                     EDGIVVVSEGEPGALSLARASGKTVVTYGVDVGQFRAANIDYGSTTTFTLLRPDGGDI
                     DLSMQIIGRHNIENIVGAAAYVLSRDLVSPEALQAAVASFTGVRRKLDNIAPASRVPV
                     YEGFGSSYEKARSAIDAIRLHYPDKRLVVVFEPHTFGWRNRANLPWYDDVFAGSSLVF
                     VAPPETQGAGTHDQLTHGEIMARIATTGVPAKPYSPAGFIETVEELEEDDVVLILTSG
                     SLEGTLEELAAAITARFS"
     gene            complement(10114..>10338)
                     /locus_tag="VEA36_02900"
     CDS             complement(10114..>10338)
                     /locus_tag="VEA36_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29286.1"
                     /translation="EFNKRGIEAVNVTVYAFFGKPRTAGDYYDRIETACRQLGMGVQC
                     QLRLQKDASYGYAEHPTLHDPNLGIFMRLM"
BASE COUNT         2117 a         2949 c         3301 g         1971 t
ORIGIN      
        1 accgagctcc ggttcaattc gatcggtcct tcgcttgggc gtgagctcac taccaaggcg
       61 ttgtacgcca tcggggcggt gctgcttgcg atcatgctct atatcgcctt cgccttccgg
      121 aaggtctcgc gcccggtgcc cgcgtggggg tacggcatca cctcggtcgc catgctcgcg
      181 atcgacatca tcgtgccggc tgggttcttc gcagtttacg cttacttcac gggcgcgcag
      241 gtggatacct tattcgtcat cgcgctgctt gccctcatgg gctactgcat caacgacgtg
      301 atcgtcatct tcgaccgcgt ccgcgagcac ctccggtgga acgaggagcg caacgtgaag
      361 gagccgttcg aggagacggt ggggaagtcg atcaccgaga cgctgggccg ctcgatcaac
      421 acctcgctca cggtcgcgct cgcgcttctc gcgatcatcc tcgtcgggag cgagtccacc
      481 cggatcttcg cgctcgtcat gctcgtgggc gtcgtggtcg ggaccttctc ctcgatcgtc
      541 cgttcggccc cgctcctgat ccccatcgca acatgggccg agaagcgcga ggcagctaaa
      601 aagaaggggt aatatagacg catatgctca tcggtatcac cggaacgaat gggtcaggaa
      661 aggggacggt ggtggaatac ctcgtcatgc ataagggctt ctcccatttc tccgcccgca
      721 tgttcatcat tgaggaggtg gtgaagcgcg ggcttaagca gaaccgacgg acgatgcgcg
      781 aggtggcgaa cgagatgcgc aaggagcaca gtccctccta cctcatggag cgcctctacg
      841 aaatggcgaa ggacgagccc aaggcggtgc tcgagtcggt caggacaatc ggcgaggcgg
      901 aatttctgaa ggcgaaggga gtctatctgt ttgcggtaga tgcggaccgt acgctccggc
      961 atgagcgcat cgtggcgcgc ggggccgaag gggacaacct gagctacgag gagttctgcg
     1021 agcaagagga tgccgagatg gcgtccccgg acccctggga catgaacgtc ttcggcgtga
     1081 tgcagatctc ggacgcgcgc ctcatgaata acggcacggt cgaggacctg catcaccagg
     1141 tcgacgaggc gctcgctgca gtgcagaagg tatgaggatc gtcgggatcg ctggttcctt
     1201 tggtgccggg aagggcgtgg tcgtggacta tctcacgcag aatctcggct tcaatcacta
     1261 ttcggcaagc ggctttatca ccgaggagat cgtgcgccgc gggatgccgg tcaatcggga
     1321 ctccatgatc gtggtcgcca acgacctgcg ggcgacgaac ggcccaagct acctcatcga
     1381 ctccctattc gagcgggcga aggaaggcgg gaaggacgcg gtcattgaga gcctgcgcgc
     1441 ggtcgccgag gtccggcgca tcaaggagct cggcgggttc gtgctcggag tggatgccga
     1501 gccgcggctt cgctacgagc gggccgttaa gcgcgggagc gagaaggacg cggtctcgta
     1561 cgagaaatgg ctcgagcagg agcgggcgga atcgaacccg gatgatccga cgaagcagaa
     1621 catcttcggg gcgctgaaag aggcggatgc gatcgtatcg aataacggca gcctcgagga
     1681 gctgtacaag caggtggacg aggtgctcgc cgccataacg tagaccatat gccgcacatc
     1741 cacgagaaga tcgactatgc tggcgatgtc ttcatcgtga acgacggcgc tgtgctgctg
     1801 cgcatgcacg ataaatacca tatgtggctg ccgcccggcg gccacgtcga gctcgacgag
     1861 gatttcgtag aggcggcgct tcgcgaggcg aaggaagaga ccggtctcga cgtgaagctc
     1921 ataggagaca acgggacccc gacggaatca acgggtaccg atatctacgg cgagcgcgag
     1981 ctcctcatac cccggttcgt caaccgccat cgcattaacg agatgcacga gcatatctcc
     2041 ttcatttatt ttggagtgag cgacacgcgc gaggttaacc cagcggaagg ggagaagcgc
     2101 gactgcttta agtggtttac cgccaaggaa ctggatgatc catcctacgg tgtgaacgag
     2161 cgggtgagga aatatgcgaa ggcggcgctc gatgccgcag cctagtatgg attttgcggt
     2221 cacagtcctg ccggtcctca gcgggctgcg ggatatcctc atgccgcact gggggaacgt
     2281 cgaggtgctg aaggagaagg gggcgggggc gcacgacatc gtcaccgagc tcgatatagc
     2341 ggtcgaggag cgcacccgca ccgagttggc ggaactctac ccggacatcg ccttcgtggg
     2401 cgaggagggt ggcggggacc ggaccgccga ccggctctgg ctcatggacc cgatcgatgg
     2461 gaccgggcat tatctgcgcg ggctgccgtt ctgcacctcc atgctcgcat atatcgaagc
     2521 gggacaggtc cgcttctccg ccatctacga tttcgtgaac gatcgcgtgt actgggccga
     2581 gcgcggcaag ggcgcgtact gcaacgggga taggctcggg gtcagcaacc ggaaactccg
     2641 ggaatcatat gtcgcctggg agtccaatca gtccgaagcg gccaaccgcg agctcaccca
     2701 gctcctgcgg caggagacca atctcttcaa ctcgatcaat gccggctggg agatggcgat
     2761 ggttgcctcc ggcaagctcg atgcacgtat ctgcttcgag ccctatggca aggactatga
     2821 tttcgccccc ggctccctcc tcgtcgagga ggcgggcggc gtggtcgcga acgtaggaac
     2881 gacgacctac gactaccgca acactagctt catcgccgct gggccgacgc tctatcagga
     2941 gctgaccgaa ggggaacggt cgatcttccc gattacgaga taaccgtccc cgtgaagggt
     3001 tggtgcgaga gatacttatc gaactcttcg aggttcccgc cgttcatgat ggcgacctcg
     3061 agtccgatct tctcggcctc gcgcgccgcg accggatcga agggggaaga gaggcccggg
     3121 tcccattccg cggggatgag cttccggaac tcggcccagc cgatcgtctc gattggggtg
     3181 gccgacgggt cctgcttcgg gtccgccgtg tacacgtgcg tgatattcga aaggttcacg
     3241 agcttcttcg cgccgagcgt cttcgctgcg atgacggcgc agtaatcggt ggaccagccc
     3301 ggcttccagc ctgctccaat gatgaccggc atcttcgccc ggatgcggtg cgatggattc
     3361 tttacgatgc gcgcctgcgc ctcctccttg aagacggcgc gcatgaggtg ggcgttgagg
     3421 cgcgaggagt ggatgccgag ccaatcgaca tcgtcgcggt tgagcccgcc gcgcacctcc
     3481 gcgaccgcgc cctgataatt gcgggcggtc ctgccgccgc cggtgatgat gaaaaaagag
     3541 agccctgcac gggttttgga aacaattaac tcacggaacc gttttaaaaa ggaaacgtcg
     3601 atgccgtcgg gaacgataag cgagcctccg acggagacga cgacgtactc acgcttagcc
     3661 cggatgaatc tcatgcaaag cttgacggct cctcccatca tacctccgtc cacctcgacc
     3721 gcaacttgtg gatgagggaa agagtttcct aattcggacg ccttgcggat ccgcaagatg
     3781 cgtatgcagc gttcccagac gcaaaccagg agggctacgc ccatgacgac gcttgcagaa
     3841 gtaggggaat cgcgggcccg cctacgcacc cgcactcctc gtgaccaacc cgagcatctg
     3901 actgcggtct ggcacgaggc cgacggcgtc aggtacgctc gggccacact ttccgccctc
     3961 gggttctcca ccccgcccct atgggcagag gtgctcgaca gggtcagggc ctacggcggg
     4021 cactgcgact tcgcagacct gccgcgcatc ggggaaggcc ttatcgagct tgcggacgcg
     4081 gtccggccgg ccatgccggc gcagggcggc gagatcgtgg tgtgcatcgg cgacgccagc
     4141 gtcggcaagg tgcgcgtgcc aatcgagcct gcccggcaca tctccctcga cgtcgaggtc
     4201 gcctaccgcc tcgccgccta gacctccgga aggccctacg ttccttaggc cccgtctcca
     4261 aggacggggc ctaagcacac cctatttcgt cccttttggc cgtgcttgac cccccggacg
     4321 gcttcctata gtatgtgcct atcgcctcca tgtggaggcg ggctagtttt cgcacgttga
     4381 aaattacata gttcagtaag gtagtgaact taagaaatct attttcttgt gttccgccta
     4441 ttcttaatta gagtttgatc ctagctcagg atgaacgctg gctgtatgga taaggcatgc
     4501 aagtcgaacg gcccgcaagg gcagtggcag acgaggtagt aacacgtagg tacgtactcc
     4561 caagtccggc atagctcgtc gaaagacggg gtaattccgg atggttcccg caagggtaaa
     4621 gatttatcgc ttgggaagcg gcctgcgtag tatcagctag ttggtagggt aatggcctac
     4681 caaggctacg acgcttaggg gagctgagag gctgaccccc accgatggga ctgagacacg
     4741 gcccatactc ctacgggagg ccgcagtcga gaatcttccg caatggacga aagtctgacg
     4801 gagcgataca gcgtggtgga cgaagtgctt cggtacgtaa acacctttta tgtgggacga
     4861 aatctttgac ggtaccacat gaataagggg ctcctaactc tgtgccagca ggagcggtaa
     4921 tacagaggcc ccaagcgtta tccggaatca ttgggcgtaa agggtgtcga ggcggttatg
     4981 ttagtctttc gtaaaatcct ggagctcaac ttcaggcccg cgagagatac ggcataactt
     5041 gagggtggga gaggtgcgtg gaactcacgg tgtaggggtg aaatccgttg atatcgtggg
     5101 gaacaccaaa ggcgaaggca gcgcactggc ccattcctga cgctcacaca cgaaagccag
     5161 ggtagcgaac gggattagat accccggtag tcctggccct aaacgttgct cgctcgttct
     5221 gcggagtatc gaccctctgc gggactaagc taacgcggta agcgagccgc ctgggtagta
     5281 cgagcgcaag cttaaaactc aaaggaatag acggggactc gcacaagcgg tggatcatgt
     5341 ggcttaattc gtcgataagc gaagaacctt acccaggcta gaaaccaagc tgcacgcctc
     5401 ctgaaaggga ggaagccttc gagggtgctt ggcaggtgat gcatggttgt cgtcagttcg
     5461 tggcttgagc tgttcccttc agtggggtaa cgaacgcaac ccacgttgtc tgttatatat
     5521 gtcaggcgag actgccccct cacgggggag gaaggtgtgg atgacgccaa atcagcatgt
     5581 cccttgatgc ctggggctgc acacgtgata caatggatgg tacagaagga cgcaataccg
     5641 taaggtggag caaatcccca aaaccatccc cagtacggat agaggtctgc aaccgacctc
     5701 ttgaagccgg aatcgctagt aatcctgggt cagccatacc agggtgaata cgttctcgag
     5761 tcttgtactc attcaaaaaa gtgcttggta gttaagcaat ccagtccgtt gaaatcggac
     5821 catctgcgtg gccgcaggtg tagtgaacgg acagtcgaga gaccgcgagg tctgcggcag
     5881 agagtcccag tagcaaaaag cagccctcct agagagatct agagcgtatg taggacccat
     5941 tctggtgagt aatggggaac tacttaacaa tcgcgctgag cggatgcaag gaacggaatg
     6001 cttaatgacc caaggagatc tcatagaggc agaggtgtta tgcacactct atgagaagtc
     6061 agcagtgtcg tagtactagg cgtcaactta taattgacgc atgggaagga cactaccgac
     6121 agtcagcaac gactacgttg tcgggcttac ggatggcgag gggtgcttct atgtcaacat
     6181 atggaagtct tccgcctacc gagcaggcgc gggcgtgcaa ttgcattttc atctgaagat
     6241 gcaggagcgc gaccgggatc tgctcgaagc agtgaaaaac acgataggat gcggcgcggt
     6301 gtacttccaa aaagaacaac gcaccaatca ttgtcagtgt taccgctaca ccgtaagcgc
     6361 gacgcgggat atcttgagca cggtaatacc gtttttcaag caatatccgt tgcaaagtgc
     6421 gactaagcga gcgagtttcg agctcttctg tcagattgcc caactcgtgc aagcgggaag
     6481 gcatcggaca atcgaaggac tcgaagagat tcgcgtgctg aaatcgcgca tgaaccaaaa
     6541 gactgttggg ctcgcgtagt acgggaaatc cgtacgctac gtgggaacgc gaagtaacct
     6601 caactatcgc aatccgcccg tcaactcaag gaagctgggg gtacccgaaa tcttgcttta
     6661 cgcaggacta cggtaaattc agtgacaggg agtaagtcgt aacaaggcac ggctagcgga
     6721 agctggtcgt ggaacatatc caatcggaat cgagtcgtgg ggaaccacgc tcatccggtc
     6781 gatcgtcagt cttcggactg atggggagat gactgttgtc cctaaacaca gtccgaccgc
     6841 cggaacaacg ttaccggtga ccaggtggga gcgctcaatt gacgctctca cggtcgaata
     6901 ggcggaacac aagagtgcgg atttctttca ctaccgaacc agcagagccc cgtgtgggag
     6961 accgatcctc ggaccgatct cccacacggg gctttcgcta tgccatcgcg ggctcctgcg
     7021 tctcgatgag ctgctgcgcg atccgtctga ccgcgcggcg ccagatgcgc gccttcctcc
     7081 ggtgccgctt tatcttgtca tcaatcgtgg agatccgatt gacccggatt ttcttaggtt
     7141 cacgtgtgtt accacggtgt accattcttg acttccagta tatccgacgt acctgaagaa
     7201 aactggaagg ccctggggat ttctggtagt atcccagggt tcgcaagtgt agctcagttg
     7261 gtagagcact cgactgatac tcgagcggtc gtaggttcga atcctaccac ttgcaccgta
     7321 atacccagcc gggggctgga ttcatgaagc gggaggtata ctttccggta tgaggcactc
     7381 catcatcgtg ctcgtgctct gcatcctcgc gatcctcgtc ggggcctacc tcttctatac
     7441 caatacgcag ggcctcggcg ccgataccct cgagcagtcg gcccaggtcg cgaacgcgcc
     7501 cgtcgagtca atgccggtcg cgttcacggt tatcgagtca ggcacctcgg cctcggacgt
     7561 taccgtacgc aagaacgtcg cggcgcgcga tcaggaagca tacctgcggc tctatgcgct
     7621 cgcgcgcggc gggcaggagc ttgcgccgcc gcccgtggat ttcaatacag aatacgtcat
     7681 cggcgtcttc gccggcgaga agccgaccgg cggccatatg atcgaggtga cgagcatccg
     7741 cgatctcggg gatacccgga cgctctccgt gaccgagacc gttccaggcg cgagctgcgt
     7801 cgtgacgcag gccttcacgg cgccctacca gctcatccgc gtgccggtga gcatgcacta
     7861 cctcaaggcc gtcgatacga gggtcgcaag cgactgccta tagcttaggg cgaggcgagg
     7921 cggtcgggga atgagacggt gcgctccgca tcctccttga tgcgctgctt gaggagcgtc
     7981 gggtttgcgt tccatttcgt gccgtcccag aactcgagcg gctcgaggtt cattttgtac
     8041 tttcccttat cgccgtacgc ggtcccgagg tacgcatacg ctttcccgcc tgccttcgcc
     8101 tgccgcacgc tatcgagcat gagccacatg cccagcgatt tctcgacgta ggcgaggtcg
     8161 tagcaggagt accagtaatg gatgaaggtc tctccgacga cctcgagcac gtaggccacc
     8221 ggcccggtct ccgtgtcata gcggacgcct cgaagcggga gcgcgctctc caggatgccg
     8281 aggacccgcg ctcggggcat gctgctgtcc gtatggcggc cggcgaaata cgcgaggaag
     8341 caatcgagga acgccgggtc ctgctggagc gcttcgcgcg agagaagggt acgggtgaac
     8401 acgccatcga atttcttcag gatgcggcga ttctcgctcg tcgtggacca ggcctgcagc
     8461 ggcacccgaa cgctccggca gaggtagagg aggttatgca ctgaagggtc agtggaatag
     8521 gggaggaagc cgagggcata ggcctcagcg ggatcgtcct ccggcgccag ctcggcgtac
     8581 gaggtgtagc cgaaggcata gaacctgccg taggcatgcc cgaattcagt ttggaagatc
     8641 cgcataccct aggagaaccg tgcagttatg gcggccgcga gctcctcgag cgttccttcg
     8701 aggctgccgg aggtgaggat gagcaccaca tcgtcctcct cgagctcttc gaccgtctcg
     8761 atgaagcccg cggggctgta gggcttcgcg gggacgccgg tcgtcgcgat acgcgccatg
     8821 atctcgccat gggtgagctg gtcgtgggtg cccgctcctt gggtctcggg cggcgcgacg
     8881 aagacgagcg agcttcccgc gaatacgtcg tcgtaccagg gaagattcgc gcggttcctc
     8941 cagccgaagg tgtggggctc gaagacgacc acgaggcgtt tgtcggggta gtggaggcgg
     9001 atcgcatcga tcgccgagcg cgccttctcg taggaggagc cgaagccctc gtagacaggg
     9061 acgcgggagg cgggcgcgat gttgtcgagc ttgcggcgga ctcccgtgaa ggaagcgacg
     9121 gcagcctgga gggcctcagg ggacacgagg tcgcgggaga ggacgtaggc cgcggcgccc
     9181 acgatgttct cgatgttgtg ccgcccgatg atctgcatgg aaaggtcgat gtccccgccg
     9241 tccggacgaa gcagcgtgaa ggtcgtcgtt gagccgtaat cgatgttcgc cgctcggaat
     9301 tgcccgacgt ccacgccgta ggtcacgacg gtcttgccgg atgcgcgggc gagcgaaagc
     9361 gcccccggct ctccctccga caccaccacg atgccgtcct cgggaacgag cgcgagcagg
     9421 tccgtaaacg gccgttggta gctctcgaag gtcgggtaga cgttcacgtg gtcgtgcacg
     9481 accgcggtca ggaggatgtc gcgcggatgc aggtgcatga acttcgcgcg cgggtcgtca
     9541 tgggcggatg gatactcatc gccctccgcg acgaagacgg gatggctccc gagccccgcg
     9601 ggcaggggaa gggaaagcgg ctcgccgccg atgaagtacc cggcgtccac gccggagtgc
     9661 cgcaggatgt gcgcgatcag ggacgtggtc gtagacttgc cgtaggagcc ggcgaccacg
     9721 atgttctgcc gctctctcgt gagctcgccg aggacttcgg ggaaggagag gatgggcttg
     9781 ccggtattgt gagcggcctt cacctcctcg ttgacatccg gggcgagctt ggcgttccgg
     9841 ccgatcacga atcggtcgac gtacggggag atgttgtccg gggtatagga gagccgcggc
     9901 gcaaggccgc ccttctcgag gatgcgcttc ggagggccgt agcactcggc atccgagccc
     9961 gtgacctccc agcccgcctc cttcagaagg agcgcgaccc cgctcatccc gatgccgcag
    10021 atcccgacga aatggacgct cttcttttcc ggcatcggtc tatagtacca aaaggtttgg
    10081 ccggccccca caatcgggag gccggccgcg cgactacata agtcgcatga agatgcccag
    10141 gttcgggtca tggagcgtcg gatgctcggc gtagccgtag ctcgcgtcct tctggaggcg
    10201 gagctggcat tgcaccccca tacccagctg acggcaggcg gtttcgatgc ggtcgtagta
    10261 gtcgcctgcc gtacgcggct tgccgaagaa ggcgtacacg gtgacgttca cagcctcgat
    10321 gccgcgcttg ttgaactc
//
LOCUS       DASPKG010000069         3263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_375166, whole genome shotgun sequence.
ACCESSION   DASPKG010000069 DASPKG010000000
VERSION     DASPKG010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375166"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VEA36_02905"
     CDS             complement(<1..484)
                     /locus_tag="VEA36_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29287.1"
                     /translation="MDEPTVPQSGARRWWVVAAAVAVLAAAGAGSYLLLTRQIPSLPA
                     PLSQATGDSLGFYFYDSQYSDSESGAAATVTYSIYRLPTKLEDREVPVFTGEFNNTTR
                     FITSSGKPGTVLFIVSSDTESLVQAIDLISPAPRLETIHTFPVTSIGERVTNARFIDD
                     G"
     gene            complement(522..595)
                     /locus_tag="VEA36_02910"
     tRNA            complement(522..595)
                     /locus_tag="VEA36_02910"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(561..563),aa:Gly,seq:ccc)
     gene            complement(601..957)
                     /locus_tag="VEA36_02915"
     CDS             complement(601..957)
                     /locus_tag="VEA36_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29288.1"
                     /translation="MPRKPKKKVGQYALAAVLALSIIWFSWLVFGIARKEEVARRAVA
                     ETKAELASLKDRESVLQANLDELSTERGQEATLRQTYGVARPGEEVIIVVPPEEEPEK
                     VELPWYKKFLGWFGIW"
     gene            complement(979..2769)
                     /gene="lepA"
                     /locus_tag="VEA36_02920"
     CDS             complement(979..2769)
                     /gene="lepA"
                     /locus_tag="VEA36_02920"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HYF29289.1"
                     /translation="MNIRNFSIIAHIDHGKSTLADRLLERTGTIEARKMRDQVLDKMD
                     LERERGITIKMQPARMKYHGKDGAEYVLNLIDTPGHIDFAYEVSRALSAVEGVILLVD
                     STQGVQAQTLTTLSMAQAAGCVIIPVLSKIDAPASRPDDVRLELAELLAVDPDTVLMV
                     SGRTGEGVEDLLEAIVERVPAPRPSEGKDSRALIFDFSYSSHRGIAVFCRVFDGSFTK
                     GDVLRFAAADQKFTVLETGIFMPDELATERLVAGEIGYIVTGVKEAGVVAVGDTVLTD
                     KGSREPLAGFSRPRPVVWASVYPESQDDLTELRQSLERLRLSDSSLSFEEESSGVLGR
                     GFRCGFLGMLHLEIVTERLRREFNLTLIVTIPTIAYHVTTKGGKRETIYTPSKFPAFG
                     DIELIEEPWVKVVLITPAESLSGMIQLFQDHEGAIMETETHMDGRIELAVEMPLRELM
                     RGFFDKVKNASSGYASLSYEFLDDRPADVVKLEIFVAEEPVPAFARVVARRRVQEEAE
                     RMVEKLEKILPRQMFVLKIQAHALGRIIASKSISALRKDVTGYLYGGDITRKMKLLEK
                     QKKGKKRRGAHSKMEIPQDVFMKMVQDSER"
     gene            complement(2792..>3263)
                     /locus_tag="VEA36_02925"
     CDS             complement(2792..>3263)
                     /locus_tag="VEA36_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29290.1"
                     /translation="GAVVALATVQTFPTARAFMEVLLRVEGVPGTGILAVALGATLGQ
                     LAMAAIALLTLRQVAPGVAGSLSRALGEGLAAGILGASAAYGVLSYFGTLAPLTTLAV
                     VFAEGLLAGMVGLAVAAAVLALLANQEFRELIDSLKKISSRSLKPHAVIENTTD"
BASE COUNT          610 a         1065 c         1022 g          566 t
ORIGIN      
        1 taccgtcatc gatgaagcgc gcattcgtca cccgctcccc gatcgacgtc accgggaacg
       61 tatggatggt ctcgagccgc ggtgccgggc ttataaggtc gatcgcctgg accagggact
      121 ccgtatcact cgagacgatg aagaggaccg tgccgggctt gcccgaactc gtgatgaatc
      181 gggtcgtatt gttgaattcc cccgtgaaga ccggtacttc gcgatcctcc agcttcgtcg
      241 ggaggcggta gatgctgtag gtgacggtag cggccgcgcc cgactcagag tccgagtatt
      301 gcgaatcata gaagtagaag ccgagcgagt ccccggttgc ctgcgacagg ggtgccggaa
      361 gcgacggtat ctgcctcgtg aggagcaggt agctccctgc cccagcggcc gcgagcaccg
      421 cgaccgcggc agcgaccacc caccagcggc gggctccgga ctgcggtacg gtcggctcat
      481 ccatatggtg aatcataaca ggcatcgcac attgctgctg atggtgcgga tagggagaat
      541 cgaactcccg cccagtgctt gggaagcact tattctgcca ctaaactata tccgcgtgag
      601 ctaccagatc ccgaaccagc cgaggaattt cttgtaccag ggcaattcca ccttctccgg
      661 ctcctcctcg ggcggcacga cgatgataac ctcctcgccg gggcgcgcaa ctccgtaggt
      721 ctggcgtaag gtcgcctcct gcccgcgctc ggtcgaaagc tcatcgaggt tcgcctggag
      781 gaccgattcc cggtccttca aggacgcgag ctcggccttc gtctccgcga ccgccctgcg
      841 cgccacctcc tccttccgcg caatgccgaa aacgagccag gagaaccaga tgatggaaag
      901 ggcgagcacg gcagcgagcg cgtactggcc gaccttcttc ttcggcttac ggggcatgga
      961 ccccagtata ccaagcggct agcgctcgga atcctgcacc atcttcatga atacgtcctg
     1021 cgggatctcc atcttcgagt gcgcgccgcg acgcttcttc cccttcttct gcttctcgag
     1081 cagcttcatc ttccgggtga tgtcgccacc atagaggtac cccgtcacgt ccttgcggag
     1141 cgcggagatg ctcttggagg cgatgatgcg ccctaaggca tgcgcctgga tcttcagcac
     1201 gaacatctgg cgcgggagga tcttctcgag cttctccacc atgcgctccg cctcctcctg
     1261 cacccggcga cgcgcaacca cccgcgcgaa ggccgggacc ggctcctcgg cgacgaagat
     1321 ctcgagcttc accacgtccg cgggacgatc atcgagaaat tcgtaggaca gggacgcgta
     1381 tcccgaggag gcgttcttca ccttatcgaa gaagccgcgc atgagctccc gcagcggcat
     1441 ctccaccgca agctcgatgc gcccgtccat atgtgtttcc gtctccatga tcgcgccctc
     1501 gtggtcctgg aagagctgga tcatgcccga gagcgactcg gcgggggtga tgagcaccac
     1561 cttcacccat ggctcctcga tgagttcgat gtccccgaag gcggggaact tggagggcgt
     1621 gtagatggtc tcccgcttcc cgcccttcgt ggtcacgtgg taggcgatcg tcgggatggt
     1681 gacgatgagc gtgaggttga actcccgtcg cagccgttct gtcacgatct ccagatggag
     1741 catgccgagg aatccgcagc ggaatccccg gccgagcacc cccgatgatt cctcctcgaa
     1801 cgagagcgag gagtccgaga gccggaggcg ctcaagcgac tgccggagct cggtgagatc
     1861 gtcctggctc tcggggtaga cggaagccca gaccacgggg cgcggacggg agaagccggc
     1921 caagggctcg cgcgatccct tatcggtaag caccgtatcg ccgaccgcga ccacgcccgc
     1981 ctccttcacg cccgtcacga tgtacccgat ctcgccggcc acaaggcgct ccgtcgcaag
     2041 ctcgtccggc atgaaaatgc cggtctcaag gacagtgaac ttctggtccg cggccgcgaa
     2101 ccggagcacg tcacccttcg tgaacgatcc gtcgaagacg cggcagaaca ccgcgatgcc
     2161 gcgatgcgag gaataggaga aatcgaagat gagcgcacgg gaatccttcc cttcgcttgg
     2221 ccgtggggcc ggcacgcgct cgacgatcgc ctcgagcaga tcctcaacgc cctcgccggt
     2281 acgccccgac accataagca ccgtgtccgg gtctaccgca aggagctcag cgagctcgag
     2341 gcggacgtcg tcgggccttg aagccggcgc atcgatcttt gagagcacag ggatgatgac
     2401 gcagcccgcc gcctgcgcca tggagagggt cgtcagggtc tgcgcctgca cgccctgggt
     2461 ggagtcgacg aggagaatga cgccctcgac cgcggagagc gcgcgggaga cctcgtaggc
     2521 gaagtcgatg tgccctggcg tgtcgatgag attgagcacg tattcagcgc cgtccttccc
     2581 atggtacttc atccgtgccg gctgcatctt gatcgtgatg ccccgctcgc gctcgaggtc
     2641 catcttgtcg aggacctggt cccgcatctt ccgcgcctcg atggtaccgg tacgctcaag
     2701 aagccgatcg gcaagtgtgc tcttgccgtg atcgatgtgc gcgatgattg agaaattgcg
     2761 tatgttcata tggatgcacc gccccggttc cctagtccgt cgtattctcg atgacggcgt
     2821 gcggcttgag cgatcggctg ctgatcttct tgagggaatc gatgagctcc cggaactcct
     2881 ggttcgcaag gagcgcgagc accgccgcag caacagcaag gcccaccata ccagctagga
     2941 ggccctccgc aaagacaacc gcgagcgtgg tcaggggcgc gagcgtgccg aagtacgaaa
     3001 gcacgccgta agccgcggac gcgccgagga tgccggcagc gaggccttcg ccaagcgcgc
     3061 gggacaagga gccggcgacc cctggcgcaa cctgccgaag cgtaagcagc gcgatcgcgg
     3121 ccatcgcgag ctggcccagg gtcgcgccaa gcgcgaccgc aaggatgccg gtgccgggca
     3181 cgccttcgac gcgcagcagc acctccatga acgcgcgggc cgtcgggaag gtctggacgg
     3241 tcgcgagcgc caccaccgcg ccg
//
LOCUS       DASPKG010000070         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1863955, whole genome shotgun sequence.
ACCESSION   DASPKG010000070 DASPKG010000000
VERSION     DASPKG010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863955"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /gene="sppA"
                     /locus_tag="VEA36_02930"
     CDS             <1..615
                     /gene="sppA"
                     /locus_tag="VEA36_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HYF29291.1"
                     /translation="VTASDEVIEYLRTARENETIKGVLLDIESAGGYPVPSEEIAQAV
                     AGVGKPSVAWLRQVAASGAYWVASAADTVVASANSDVGSIGVSMSYLDYAAQNETDGL
                     TFNSLASGKFKDSGSPDKELTEEERALFERDITIMHDNFVSAIATNRNLPKETVSALA
                     DGSTMLGAMAKEQGLVDQLGGQTEAYAALEAAIGEKPVICWAGL"
     gene            complement(697..1071)
                     /locus_tag="VEA36_02935"
     CDS             complement(697..1071)
                     /locus_tag="VEA36_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29292.1"
                     /translation="MFFALALSGMFMPDDPLYWLGDLRPTLATMAAYVFLFGSVSFFL
                     YRGRKPGHKTRAICFTVGIAMPIALAERAFAQDGMDFVSSTLLMLLHTWVLTSCLSLT
                     VTAVWSSWERDEAGSTRSSRVP"
     gene            1200..1493
                     /locus_tag="VEA36_02940"
     CDS             1200..1493
                     /locus_tag="VEA36_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004475053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HYF29293.1"
                     /translation="MTRYTFTTQVWLYPGMAGWHFASVPEDVSAKIKKSREGLPRIGW
                     GSVPVTATIGNTSWKSSIFPDKKSGTYLLPLKAEIRKKEGIAADDTITLTLEL"
     gene            complement(1490..1948)
                     /locus_tag="VEA36_02945"
     CDS             complement(1490..1948)
                     /locus_tag="VEA36_02945"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HYF29294.1"
                     /translation="MKKMYWGIGLIGAAAALAFGWYAVSPLFNPLKTDEALPESAAPM
                     MAETREMAGTIVGTPGHPSSGTVRIVSADGKQYVRYENLKTINGPDIYVYLSKDLGAR
                     EYVELGKVKSTEGNANYEIPAGVDPADYRYVLIWCKQFGVLFNSAEINPA"
     gene            complement(2017..2577)
                     /locus_tag="VEA36_02950"
     CDS             complement(2017..2577)
                     /locus_tag="VEA36_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29295.1"
                     /translation="MSGGITVIVPVAHDLDTVDGSVICGTGTMKACDHALALMKRFPR
                     SQLFVSAREAPRFGNVVMGEVMRAYLLKKLPDAITSFLKAKYFNTLGEVLATYEHVHE
                     LRQMYGPQAVERVVFAVKDWHARRVDKLARAVFTHYRLDVPVCIKVHHVPEAPPTRRE
                     RLLEPIKTACFLWFFHHVHLKKQAEA"
BASE COUNT          571 a          878 c          841 g          491 t
ORIGIN      
        1 gtaaccgctt ccgacgaggt catcgaatac ctcaggaccg cgagagagaa cgagacgatc
       61 aagggcgtgc tcctggatat cgaatcagcg ggcgggtacc ctgtgccctc cgaggagatc
      121 gcgcaggcgg tcgcaggcgt agggaagccg tcggtcgcat ggctgcggca ggttgccgcc
      181 tccggcgcct actgggtcgc aagcgcggcc gatacggtcg tcgcctccgc taactccgac
      241 gtcggcagca tcggcgtctc catgtcctac ctcgactacg ccgcccagaa cgagacggat
      301 ggcctcacct tcaacagcct cgcctccggc aagttcaagg acagcgggag ccctgacaag
      361 gagctcaccg aggaggagcg cgccctcttc gagcgggata tcactatcat gcacgacaac
      421 ttcgtctcgg cgatcgcaac caaccgcaac ctcccgaagg agaccgtatc ggcgcttgcc
      481 gacggctcca ccatgctcgg cgctatggcc aaggagcagg ggctcgtgga tcagctcggg
      541 ggccagaccg aggcatatgc cgcgcttgaa gccgcgatcg gagagaagcc ggtcatctgc
      601 tgggcggggc tctaaccagt tagctcgacc tctacgttac gaaaacggcc gcccggtgag
      661 gggcggccgt gtggcgtcgg agatgttcgt cgaggatcag ggaacgcggg aggagcgcgt
      721 cgagcccgcc tcgtcgcgtt cccagctgct ccatacggcc gtcacggtca ggctgaggca
      781 tgacgtgagg acccaggtgt ggagaagcat gagtagcgtc gaggagacga aatccatgcc
      841 atcctgagcg aacgcacgtt cagcgagcgc gatgggcatc gcgataccga cggtgaagca
      901 gatggcgcgg gtcttgtggc ccggcttgcg tcccctgtaa aggaaaaagg agacactgcc
      961 gaacaagaag acgtacgccg ccatagtcgc tagggtcggt cgtaagtcac cgagccagta
     1021 cagcggatca tccggcatga acatgccgct gagcgccagt gcgaagaaca tcgcaccaaa
     1081 gaatccgagc aggaatcgaa agatcggcat caaactctcc cttccgcgtt aggcggcttg
     1141 aggtaatcta caatatatgt ataagaagtc caattttccg tccgcactat aatttcctta
     1201 tgacccgcta caccttcacc acccaggtct ggctgtaccc cggcatggcc ggctggcatt
     1261 tcgcctccgt ccctgaggat gtctcggcga agatcaagaa gagccgggaa ggcctgccgc
     1321 gcatcggctg gggctcggtc cccgtcaccg caacgatcgg caacacctcc tggaagtcct
     1381 ccatattccc ggataagaaa tcaggaacgt acctgctgcc gctgaaggcc gagatacgca
     1441 agaaggaagg catcgcggcc gacgatacga tcacgctcac cctcgagctc taggccgggt
     1501 tgatctccgc ggaattgaac agtaccccga actgcttgca ccagatgagg acgtagcggt
     1561 agtcggcagg gtccaccccg gccggtatct catagttggc gttcccctcg gtggacttca
     1621 ccttcccgag ctcgacgtat tcccgtgccc cgaggtcctt cgacaggtac acatagatgt
     1681 ccggtccgtt gatggtcttc aggttctcgt accgtacgta ctgcttccca tccgccgaga
     1741 cgatccgcac ggtgcccgac gagggatgcc ccggcgtgcc cacgatcgtc cccgccatct
     1801 cgcgggtttc ggccatcatg ggcgcagcgc tctccggcag cgcctcatcc gtcttcagcg
     1861 gattgaagag cggagaaacg gcgtaccagc cgaacgcgag agcagccgct gcaccgataa
     1921 gcccaatgcc ccagtacatc tttttcatga ctgcattata tacccgatat gaaagcggcc
     1981 gccccttcca caagggcggc cgcagcaggc gggaggctag gcctccgcct gcttcttgag
     2041 gtgcacgtga tggaagaacc agagaaaaca cgcggtcttg atcggctcaa gcaggcgctc
     2101 gcggcgcgtc ggtggcgcct ccggcacatg gtgtactttg atgcagaccg gcacatcgag
     2161 cctgtagtgc gtgaatacgg cccgggcgag cttgtcgacg cgcctggcgt gccagtcctt
     2221 gaccgcgaag acgacccgct cgacggcctg cgggccgtac atctgccgca gctcgtggac
     2281 gtgctcgtag gtcgcgagca cctccccgag cgtattgaag tacttggcct tcaagaacga
     2341 ggtgatcgcg tccggaagct tcttcagcag gtacgctctc atcacctcgc ccatgacgac
     2401 gttgccgaag cgtggcgctt cacgggccga gacgaacagc tgcgaacgcg ggaaccgctt
     2461 cataagcgcg agcgcgtggt cgcaggcctt catggtcccg gttccgcaga tgacggaacc
     2521 atcgaccgta tccagatcgt gcgcaaccgg cacgatgacg gtgatgcctc ccgacatccg
     2581 aacctcctct ttgaagaacg ggtgagcccg ctaggaagag tgataccacg ggctgagaga
     2641 aggtcaacaa acgcatctga ccggctcccg gccggtcaga tgctcgtact atcctcgtgc
     2701 gtatcctcca tacgctactg gaacacgctg tccatgtatg gattcctcat gcgctcgttg
     2761 cgctcgtcaa tgacgccggc c
//
LOCUS       DASPKG010000071         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1322952, whole genome shotgun sequence.
ACCESSION   DASPKG010000071 DASPKG010000000
VERSION     DASPKG010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322952"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VEA36_02955"
     CDS             <1..278
                     /locus_tag="VEA36_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HYF29296.1"
                     /translation="VARIIRSPQDAHKINLGDILIATMSTPDFLPAMQKAAAFVTDIG
                     GITSHAAIVAREMKKPCIIGTKNATKILKDGDLVEVDADQGIVRAII"
     gene            complement(335..943)
                     /locus_tag="VEA36_02960"
     CDS             complement(335..943)
                     /locus_tag="VEA36_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYF29297.1"
                     /translation="MGRDLYCTITGMRHGNAAPPASEGDFRTVDLPRPLTELGVSQVD
                     RRRKLLGERRYSTVISSIAARARETARLVTKRGEDCMHILPCLYTPEGPDGDIMERDY
                     QRYGSKSPSYYLGHDFDGVYSKFMRVAGAEIAPFVMPEGDLIMFGHTPFTNLAVLGTL
                     RALTVEVARHIADAILGFELPEAGAFTVYVKEGEVVEFAIHG"
     gene            1073..1744
                     /locus_tag="VEA36_02965"
     CDS             1073..1744
                     /locus_tag="VEA36_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29298.1"
                     /translation="MAHHAYLVTGDIDAGIAAARAFGERELGLSGTNNPDIVILRYGH
                     FPVEEARRVVELASQSALKDSRLIVIAAERLFHEAQNALLKIFEEPPQGTTLVLVVPA
                     EGQVLQTMRSRLLPLPIAGREARALHPFVVATEAERKKIVEKLLNRSKADRDEEKQAA
                     RAETIRIVEDLTRAAHEMYLKKGSPEQAVLLAELARFTPILHERSAPLKLIFEHLLLV
                     LPRLR"
     gene            1809..>2102
                     /locus_tag="VEA36_02970"
     CDS             1809..>2102
                     /locus_tag="VEA36_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29299.1"
                     /translation="MAIIAATAAVLTNQIAPSQKPQSTTEVIQTQMQSHFSACDQEVN
                     RQVEVMVREKLAKGEPMIFPTLGPDRQLRDLSGVQLVIIGTMTCVQRKVADDLV"
BASE COUNT          430 a          641 c          660 g          371 t
ORIGIN      
        1 gcgtcgcgcg gattatccgc tcgccccagg acgcgcataa gatcaacctg ggcgatatcc
       61 tcatcgcgac catgtccacg cctgacttcc tgcccgcgat gcagaaggcc gcggcattcg
      121 tcacggatat tggcggcatc acctcccatg cggcgatcgt cgctcgcgag atgaagaagc
      181 cctgcatcat cggcaccaag aacgcgacca agatactcaa agatggagat ctggtcgagg
      241 tggatgcgga ccaggggatt gtaagggcaa tcatttaaat gaaaggagcc cccgcttgcg
      301 caggggctcc agggtagttc cggtcagcag acgatcagcc gtggatggcg aactcgacga
      361 cctcgccttc cttgacgtat accgtgaagg cgcctgcctc agggagttca aagccgagga
      421 ttgcgtccgc gatatgcctt gcgacttcga ccgtgagcgc ccgcagggtg ccgaggacgg
      481 cgaggttcgt gaaaggcgta tggccgaaca tgatgaggtc tccttccggc atcacgaacg
      541 gcgcgatctc ggctccggcc acccgcatga acttcgagta cacgccgtcg aagtcatgcc
      601 cgaggtagta gctcggtgac ttggagccgt agcgttggta gtcgcgctcc atgatgtcgc
      661 catccggccc ttccggggtg tagaggcagg ggaggatgtg catgcaatcc tctcccctct
      721 tcgtgaccag ccgcgcggtc tcgcgggcac gcgcggcgat cgagctgatc accgtcgagt
      781 aacgccgttc gccgaggagc ttccggcgcc ggtcgacctg cgagacgccg agctcggtga
      841 gcgggcgggg aagatcgaca gtgcggaagt cgccctcgga agcaggcgga gcggcattgc
      901 cgtggcgcat gccggtgatg gtgcaataca aatctcggcc catcgctgga gcctccttct
      961 cgtgctgttg ttgatgagct gacctacaac cgtaaaggtc aaggttcacg aatactatat
     1021 tacatgtatg taaataagtc aagcacactg tacgctgcgg tatcctctct atatggcgca
     1081 ccacgcatac ctcgtcacgg gagatatcga tgcgggcatc gcagccgccc gtgccttcgg
     1141 cgaacgcgag ctcgggctct cggggacgaa taacccggac atagtcatcc tccggtacgg
     1201 gcatttccca gttgaggaag cgcggcgggt ggtggagctc gcatcgcaat ccgccctcaa
     1261 ggattcccgg ctcatcgtca tcgcggccga gcgcctcttc catgaggcgc agaacgcgct
     1321 cctcaagata ttcgaggagc cgccgcaggg tacaaccctc gtgctcgtcg tgcctgccga
     1381 agggcaggtg ctccagacaa tgcggtcccg gctccttcca cttccaatag cgggaaggga
     1441 agcccgggcg ctccatccat tcgtcgtagc gacggaagcc gaacgcaaga agatcgtcga
     1501 gaagctcctc aatcgatcga aggcggatag ggacgaggag aagcaggcgg cgcgcgccga
     1561 gaccatccgc atcgtggagg acctcacgag ggcggcgcat gagatgtatc taaagaaggg
     1621 gagcccggag caggcggtgc tccttgccga gctcgcccgc ttcaccccca tcctccatga
     1681 gcggtcggcg cccttgaagc tgatattcga gcacctgctc ctcgtcctcc cacggctgag
     1741 gtgatataac tgacgataga tcccggtcca ttcggacggg tgtaagggga ggccccacca
     1801 tggcgtcttt ggcgataatc gcggccactg ccgcagtgct cacgaaccag atcgcaccaa
     1861 gtcagaagcc gcagagcacc acggaggtca ttcagactca gatgcagagc catttctcgg
     1921 cctgcgacca ggaggtcaat aggcaggtcg aagtgatggt gcgggaaaag ctcgccaaag
     1981 gggagccgat gatcttcccg acgctcggcc cggaccggca gctgcgcgac ctcagcggcg
     2041 tgcagctcgt catcatcggc acgatgacct gcgtgcaacg gaaggtcgcc gacgacctcg
     2101 tg
//
LOCUS       DASPKG010000072         5796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1052425, whole genome shotgun sequence.
ACCESSION   DASPKG010000072 DASPKG010000000
VERSION     DASPKG010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052425"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..765
                     /locus_tag="VEA36_02975"
     CDS             283..765
                     /locus_tag="VEA36_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29300.1"
                     /translation="MTVLLQRGKEKIIRRQVTGDCLSPQYYLTLGHFSGTERFTRKLL
                     AAPPEPVLKPGTFLFFGVVTLYEPFKGGIGELLPPQSNALTYRHLAELVKPEQFPEQY
                     DKFGQLPKRGELIVSPMMAGAVTLKYTCAQWHFDDPDEESVSLPERTRIIYLKPPLQE
                     "
     gene            complement(817..1989)
                     /gene="tsaD"
                     /locus_tag="VEA36_02980"
     CDS             complement(817..1989)
                     /gene="tsaD"
                     /locus_tag="VEA36_02980"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR00329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HYF29301.1"
                     /translation="MKLLAIETSCDETAIAVLEAEGNTHNARFKVLGNALLSQIDIHK
                     EYGGVYPMLAKREHAKNLVPILSSALKDAGMLMEEANNADHIREELTTLLEREPGLGE
                     ALMAFLSQTAVPDIDAVVVTHGPGLEPALWVGVNFAKALSIAWGKPLVGANHMEGHLL
                     SALAASEGDAYVIEDAALPVVGLLISGGHTQIVAMREWFTYELMGDTRDDAVGEAFDK
                     VARMLDLPYPGGPQVSKLAEEARAGNILHPQPLPRPMIGEGTCDFSFSGLKTAVMYQL
                     KKMEGLSDTQKQAIAREFEDAVADVLFAKTARALAQTHGKTLVIGGGVSGNVHIRRVF
                     EMRIKAEHPDVMLRIPARELTTDNAVMIGMSGYFRYLRGMRADTETLRADGNLSLA"
     gene            complement(1970..2833)
                     /locus_tag="VEA36_02985"
     CDS             complement(1970..2833)
                     /locus_tag="VEA36_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29302.1"
                     /translation="MERIVIFYHGGCLDGFGGAYAAWKKFGDGAEYVPLSRGEEPPYD
                     VAAGAHAYFIDFVYAKDVMARFLEVAAHVTALDHHEGAVEDIRSVPDHVYETEHSGAG
                     IAWKYFHPDAPVPSIVAYAEDIDLYRFALPETRAIITYLEVQPMNFAVWDEITRKLDD
                     PTLREEMLQKARTYAEYFQLLVDQSVDHAKLVSFEGQEVYFGTTHPMKSMKSLVGNLL
                     AKKQGPFALVVSAHPSGYGVSIRGDGTVDVSKIAQKYGGNGHPNSAGFLIPREGPFPW
                     TLIEHDEAARD"
     gene            complement(2839..2911)
                     /locus_tag="VEA36_02990"
     tRNA            complement(2839..2911)
                     /locus_tag="VEA36_02990"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2876..2878),aa:Val,seq:gac)
     gene            complement(3011..4975)
                     /locus_tag="VEA36_02995"
     CDS             complement(3011..4975)
                     /locus_tag="VEA36_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HYF29303.1"
                     /translation="MISKSTKGLLVGMLALGLLVSPAASPAFAQSSSASLQAQIQALL
                     AQVAALQAQLGVSAGASFTRDLTIGSTGADVTRLQQFLIARGHSIPAGATGYFGAQTR
                     AAVAAFQAANGIAPAAGYFGPLTRAKVNGMIVVTPGTPGTPSTPNDDDDELEGGAGSV
                     DSYELLGSLNSEEVGEDDEDVEVAGLEIEVDDGSDIELTAVRLVFDEGTADEDFEDYA
                     SEVSLWLDGEEVGRVDAEDFNDDNDWTATVSLDDGAIIRRGDIGELTVGISAQGNIDS
                     ADIGDTWTVDFRQIRFMDADGATVSEDPSTAARTFSFESFATAADVELTIREDDDEIN
                     DARTLEVDATNDMDDVEVLSFTMEAEGDSDITIDSLPITVTSTEGTGNDPDDLISALH
                     LIVDGEEIASETLSTADADNSSEVVVFDDLEWTIEEGETAEVMVTADLYSIADTLDAG
                     DTISFAFGETETNLATFDAEDESGENLGNADVTGSASSGAFDLRAAGIMVTFVSASET
                     VTANDNAADNDVGTFRITFNVQAFGSDVYLARSADATTASTITASTGSAGHLYLVEDS
                     GTATTDDLTDIVTEIGDASEASSNNFLLEEDEETELTITVVQTNDSAEDDGLYRVLLK
                     AVAWATTDTTTWNLYDFDLEDFKTDPVNLN"
     gene            complement(5262..>5796)
                     /locus_tag="VEA36_03000"
     CDS             complement(5262..>5796)
                     /locus_tag="VEA36_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29304.1"
                     /translation="IEFAPDAFGHGGDAFIAAFGEYTGVTTGPDIQLEGFGILRVDID
                     TKATEMFAANDLPGPSYINQTGGFNRPSDIVFAPDGSLYIVDWGGARITEEGLEETPE
                     TGIIWRIYKKDMHQPLRPDGPIVVPPAPTTDAEREPLVRNASELFGAIGRAFWQIAAA
                     VALLILAIWFFRRTHNR"
BASE COUNT         1065 a         1800 c         1793 g         1138 t
ORIGIN      
        1 ggcatcatga ccgtgtaggg ggtcccttcg gtcttcgaga ggagctccgg gggaagccgg
       61 tcagggttga aatacccact ctcctcccac gcggcataga cgcgcttctc ggtcgcggcc
      121 gggtcgtagg gcttcttgaa cttctcgtcc attatctatg gaatatagca gaaagcccgc
      181 gtttccgtta cggacgaatc atttgatttt cacttcaatg tgtgtcaaaa tgcatctacg
      241 gggtttggcc tcggagttca accacaagcg gcgagtattc gcatgaccgt tctgttgcaa
      301 cgggggaaag agaagatcat ccgtcgccaa gttaccggcg attgcctcag cccccaatac
      361 tatctgaccc tgggtcactt ctcgggtacg gaacggttca cgcgcaagct gctcgcggct
      421 cctcccgagc ccgtgctgaa gcccggaacg ttccttttct tcggcgtcgt cacgctctat
      481 gaaccgttca agggcggtat cggcgagctc cttccgcctc agagcaacgc gctgacctac
      541 cgacatctgg ccgagctcgt aaagccggaa cagtttccgg agcagtacga caagtttggg
      601 cagttgccca aacgcggcga gctcattgtc agcccgatga tggcaggggc cgtgacgctc
      661 aagtacacct gtgcgcaatg gcacttcgac gatccggacg aagaatctgt atcgttgccc
      721 gagcgcacgc gcatcatcta cctcaagccg ccgctacaag agtagcggct agggaggaat
      781 tcgaaacagc ccgcggagcg atccgcgggc cttttcttat gcgagcgaga ggttcccatc
      841 cgcccgcagc gtctcggtat ccgcacgcat accccgcagg tacctgaagt acccggacat
      901 cccgatcatc accgcgttat ctgtcgtaag ctcgcgcgcg gggatgcgga gcatcacgtc
      961 aggatgctct gctttaatac gcatctcgaa gacccggcgg atgtgcacgt tcccagagac
     1021 gcccccgcca atcacgagcg tctttccatg cgtttgcgca agtgcgcgcg cggtcttcgc
     1081 gaacagaacg tccgcaaccg catcctcgaa ctcgcgggcg atggcctgct tctgcgtatc
     1141 gctcaacccc tccatcttct tgagctggta catgaccgcc gtcttgaggc cggagaagga
     1201 aaaatcgcag gtcccttcgc cgatcatcgg gcggggaagc ggctgtgggt gcaggatatt
     1261 tcctgcccgt gcctcctcag cgagcttcga gacctgcggt ccgcccggat acgggaggtc
     1321 gagcatgcgc gccaccttgt cgaacgcctc gccgaccgcg tcgtcccgcg tatcgcccat
     1381 caattcgtat gtaaaccatt cccgcatcgc gacgatctgc gtatggccgc ccgagatgag
     1441 gaggccgacg acgggaagcg ctgcatcctc gatgacgtac gcatcaccct cgctcgcggc
     1501 aagcgcggag aggagatgcc cctccatgtg gttggcaccc acgagtggct tcccccaggc
     1561 aatcgaaagc gccttcgcga agttcacccc cacccagagc gcgggctcga gcccggggcc
     1621 atgcgtcacg accaccgcat cgatatccgg caccgcggtc tgcgagagga atgccatgag
     1681 cgcttcgcca aggccgggct cgcgttcaag cagggtcgtt agttcctcac gaatgtggtc
     1741 ggcgttgttc gcctcctcca taagcattcc tgcatccttg agcgcggacg aaaggatcgg
     1801 cacgagattc ttcgcatgct ctcgcttggc gagcatgggg tagacgccac cgtattcctt
     1861 atggatatcg atctgcgaga ggagcgcgtt cccgagtacc ttgaaacgcg cgttatgcgt
     1921 attaccttcc gcctcgagca cggcgatcgc cgtctcgtcg caggaggtct caatcgcgag
     1981 cagcttcatc gtgttcgatg agggtccagg ggaagggccc ctcgcggggg atgaggaagc
     2041 ccgcggaatt cgggtgcccg ttgccgccgt atttctgggc gatcttcgag acatccaccg
     2101 tgccgtcgcc gcggatggag acgccgtagc cgctcgggtg cgcggacacc acgagcgcga
     2161 acgggccctg cttcttcgcc agcaggttcc cgacgagcga cttcatggac ttcatcggat
     2221 gggtggtgcc gaagtacacc tcctgcccct cgaaggagac gagcttggca tgatcgaccg
     2281 actgatcgac gaggagctgg aagtactccg cgtacgtccg cgccttctgg agcatctcct
     2341 cgcgaagcgt gggatcatcg agcttcctcg tgatctcgtc ccagaccgca aagttcatcg
     2401 gctgcacctc gaggtaggtg atgatcgcgc gggtctcagg gagcgcgaag cggtagagat
     2461 cgatatcctc cgcatacgcg actattgaag gaacgggcgc atccggatgg aagtacttcc
     2521 aggcgatgcc cgcgcctgaa tgctcggtct cgtagacgtg gtccggcacg ctccggatgt
     2581 cctcaacagc gccctcatga tggtcgagcg cggtgacatg cgccgccact tcgaggaagc
     2641 gggccatgac gtccttcgca tagacgaagt cgatgaagta cgcgtgcgcg cctgcggcaa
     2701 cgtcgtaggg cggctcttcg cctcgcgata aggggacata ctcagcacca tccccgaact
     2761 tcttccaggc ggcgtacgcc cccccgaacc cgtcgaggca cccgccgtgg tagaagatga
     2821 cgatccgttc catgagtgtg cgcgatacag gactcgaacc tgtgaccctc cccacgtcaa
     2881 ggggatgctc taccaactga gctaaccgcg cgtcgtcagc aggatagcgt ctttaagcca
     2941 agtattcaac ctacggatac ggcgaaggcc ggcatatgcc ggccttcgct catgcaccta
     3001 cgctggtccc ttagttcagg ttcaccggat cggtcttgaa gtcctcaaga tcgaagtcgt
     3061 agaggttcca ggtggtggtg tcagtggttg cccacgcaac cgccttcagg agcacgcggt
     3121 agagcccgtc gtcctcggca gagtcgttgg tctgcaccac cgtgatcgtg agctcggtct
     3181 cctcgtcctc ctcaaggagg aagttgttcg aggacgcttc ggaggcatca ccgatctctg
     3241 tcacgatgtc ggtcaggtcg tcggtcgttg cggtgcccga gtcctcgacg aggtagaggt
     3301 ggcccgccga gccggtcgac gccgtaatcg tggacgcggt cgtcgcgtca gcgctccgtg
     3361 ccaggtacac gtccgagccg aaggcctgga cgttgaacgt gatgcggaac gtaccgacgt
     3421 cgttgtccgc cgcattgtcg ttggccgtca cggtctcgct cgccgaaacg aacgtcacca
     3481 tgatgcctgc ggcacggaga tcgaacgcgc ccgacgaggc ggagccggtc acgtcggcgt
     3541 tcccgaggtt ctcgcccgac tcgtcttccg cgtcgaaggt agcgagattc gtctcggtct
     3601 cgccgaaggc gaaggagatg gtgtcacccg catccagcgt gtccgcgatc gagtagaggt
     3661 ccgcggtcac catgacctcg gcggtctcgc cctcctcgat ggtccattcg aggtcatcga
     3721 agaccacgac ctccgagctg ttatcggcat ccgccgtgct caacgtctcg gatgcgatct
     3781 cctcgccgtc cacgatgagg tggagcgcgg agatgagatc atccgggtcg ttgcccgttc
     3841 cctcggtcga ggtcacggtg atcgggagtg agtcgatggt gatgtcggaa tcgccctcgg
     3901 cctccatcgt gaaggacagg acctcgacat cgtccatatc gttggttgcg tccacctcga
     3961 gggtgcgcgc atcgttgatt tcgtcatcgt cctcgcggat ggtgagctcg acgtcagcgg
     4021 cggtcgcgaa gctctcgaag gagaacgtgc gtgccgcggt cgacgggtcc tccgagacgg
     4081 tcgccccgtc cgcatccatg aagcggatct ggcggaagtc caccgtccag gtgtcgccga
     4141 tgtcggccga gtcgatgttg ccctgcgccg agatgcccac ggtcagctcg ccaatgtcgc
     4201 cccggcggat gatcgcgcca tcgtcaagcg agacggtcgc ggtccagtcg ttgtcgtcgt
     4261 tgaagtcctc cgcgtcgacg cggcccactt cctcgccgtc aagccagagg gagacctcgg
     4321 acgcatagtc ctcgaagtcc tcgtccgccg taccctcatc gaacacgagg cgtaccgcgg
     4381 tgagctcgat atcggagccg tcatcgactt cgatctcgag acctgcgacc tcgacgtcct
     4441 catcgtcctc accgacctcc tcgctgttga gtgagccaag gagctcgtaa gaatcgacgg
     4501 agccggcgcc gccctcgagt tcgtcatcat cgtcattcgg ggtcgacggc gtacccgggg
     4561 tgcccggcgt tacgacgatc atgccgttca ccttggcacg ggtgagcggg ccgaagtagc
     4621 cggctgcggg tgcgatgccg tttgccgcct ggaaggctgc aacggctgca cgcgtctgcg
     4681 cgccgaagta cccagtcgcg cctgcgggaa tggaatgccc gcgcgcgatc aggaactgct
     4741 gcagccgcgt cacatcagcg ccagtggaac cgatggtgag gtcgcgcgtg aagcttgcgc
     4801 ccgcggagac cccaagctgc gcctggagcg ccgcgacctg cgcaaggagc gcctggatct
     4861 gcgcctgcag gctcgcgcta gagctctgcg cgaaggcagg cgacgcggcc gggctcacca
     4921 ggagtccgag cgcgagcatg ccgacgagca ggcccttagt tgatttgcta atcatatttt
     4981 aatctcatta cggataagtc gtgctgtgta agtctgtggg agggaagttc gtcttctctc
     5041 ctgtgcgggc aggcacggca ggtggagact tcaggtctcc gcctgttttg cttttcgtct
     5101 ccttatctct atgacggatg gtcggcgtga gcctttcagg acggcagaga aaagacaacg
     5161 aatcggaacc tcggtcccga ttaccctctg atataccagg ggaaagctaa gtcttcggtg
     5221 aagcgacggt caggggagtt catggaaact ccatgagcgc attagcggtt gtgcgttctc
     5281 cggaagaacc agatcgcaag gataaggagt gcgaccgcgg cagcgatctg ccaaaatgcc
     5341 cgcccaatag cgccgaacag ctcggaggca ttgcggacca gcggttcacg ctccgcatcg
     5401 gtggtcggcg caggcggtac gacgattggg ccatccgggc gcaggggttg atgcatgtct
     5461 ttcttataga tgcgccagat aatgccggtc tccggggtct cctcgaggcc ttcctcggtt
     5521 attcgcgcgc caccccagtc aacgatgtac agggagccat ccggggcgaa tacgatatcg
     5581 gaggggcggt tgaacccacc ggtctgattg atgtaggacg ggccggggag gtcgttcgct
     5641 gcgaacatct cggtcgcctt ggtatcgata tccacccgca ggatgccgaa tccctcaagc
     5701 tggatgtctg gcccggtggt aacgccggtg tattcgccga aggccgcgat gaacgcatcg
     5761 cccccatggc cgaaggcatc aggcgcgaat tcgatg
//
LOCUS       DASPKG010000073         4827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1593771, whole genome shotgun sequence.
ACCESSION   DASPKG010000073 DASPKG010000000
VERSION     DASPKG010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593771"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VEA36_03005"
     CDS             complement(<1..441)
                     /locus_tag="VEA36_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYF29305.1"
                     /translation="MARYFITGGAGFLGINLVRHLLDKGEEVVSFDLLPFDYPERDRI
                     TEVTGDIRDYGALRDAMKGTDIVVHTAAALPLYSAEDIRSTDIDGTRNVLKAAKENGV
                     ERVVHISSTAVYGIPDHHPLSEDDALDGVGEYGKAKIAAEELCLE"
     gene            complement(504..1103)
                     /locus_tag="VEA36_03010"
     CDS             complement(504..1103)
                     /locus_tag="VEA36_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29306.1"
                     /translation="MPSLSARARLIRDLCQPIRTRNLALDLTDLDVYAAVPFNSYLKG
                     DDPDASNLAIAKVAIERMEALGVPFIGQWEHTNLIRQYSDCMVLAEYPGSDGSGQWVR
                     TDRLMKWIADTLREQFPEKEEIRVGLIGHLDHVQRVAALARYYGLTPKIDGASRRVPY
                     DPTVRPGAQDWCRTPERFLRFEKRKARPALIAFALTGLI"
     gene            complement(1166..2425)
                     /locus_tag="VEA36_03015"
     CDS             complement(1166..2425)
                     /locus_tag="VEA36_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HYF29307.1"
                     /translation="MRFSELTHDLRDATVFLDIDGTLVGDKQSDIAPDALEKIRALAA
                     HNDVYLSSNGDPFRAAALAKAAGVQALPQLRKPLPHAAVRSIERRERVVVIGDKFLTD
                     GIFARLIGAEFIKVVRLRGEADDAFARFAYLLDDLVWHAAPYLQLMRPQQWVKNSLVF
                     APIFFAGAFFVPSAFIAALVCFVAFSTLASAVYVVNDLRDLEADREHPVKARRPLPSG
                     TVSKLSAILLAIALTLVTCILLFLIPTAIPVFLVYALLNLAYTFSLKHVPVIDISLVA
                     SFYVLRVVAGGAAAAIALSPWIVLATFFVALFLVSGKRQGEFTHTVRRKVLDGYSEQM
                     LGYLLISSAVLAIAFYGLWAIFTHPSTAAAYSTILVATVFFRMLNIMTTDPARAESPE
                     LLVFKDGWTFGLTVFWTLVLAAIFYLP"
     gene            complement(2431..2784)
                     /locus_tag="VEA36_03020"
     CDS             complement(2431..2784)
                     /locus_tag="VEA36_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29308.1"
                     /translation="MAYIYLFAAFLLNGVANVLLKLGSVKGLSLSGNVPTLIVSNWQF
                     LLGLFLFAANVIFYFLALRALPLSIAYPVMVAMSFLIINGYAFFGLREGITTLQLGGY
                     ALIVIGLTLVVYRAG"
     gene            2877..3995
                     /locus_tag="VEA36_03025"
     CDS             2877..3995
                     /locus_tag="VEA36_03025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HYF29309.1"
                     /translation="MLFTLKKLALLALVALHGPARRCPERPQRVIVVQQAKLGDMICT
                     TPVFAALKRTYTDLHLAVMGNRVNEDVLRGNPHVDRYLVFTDFRSALKEVRKGGFDTG
                     IVTGPDFVSIALLILAGVGCVIAPSIEKGVTPLANSLYRSLLRFVVSLPHEEGTYAPG
                     QYLRLLEPLGIHTTDTTKVLAYTPSAHATAATYLKSIGERPKLLVGMTPSAGNTVKEW
                     PPERFAAIADHLISTYGAKVIVLGTKAERSKIDAMLEQVKEKQDVIDAGGRFDIEELK
                     ALIAALDLFIAADTGPMYIAEAFKVPTVDIVGPVDERIQPPQGPLHRVVIPPRSRPAQ
                     VTVLNAKWYDREEARKQAEATTVAAVIREVDDLIASMK"
     gene            4003..4653
                     /locus_tag="VEA36_03030"
     CDS             4003..4653
                     /locus_tag="VEA36_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYF29310.1"
                     /translation="MPGIYQIERALLEKQVRRYSSYIKGRVLDAGSGSMNRYGKLFDA
                     DEYVRLDVAPGSNVDVVGSIEEMPFTDAEFDAVVSTQVLEHVPHPHLAAKEIFRVLRP
                     GGRALITVPQWNELHEEPHDYWRYTSFGLANLFTEAGFTVLASEQRGGFFAARAQMGM
                     RYLIDRFSLYQHWWGRAFHYAFRVWGAVALALDRADRSAANRKHAIGWIFVFEKPL"
BASE COUNT          936 a         1473 c         1587 g          831 t
ORIGIN      
        1 ctcgaggcag agctcctcgg ccgcgatctt cgccttgccg tactcgccga cgccgtcgag
       61 cgcgtcgtcc tccgagagcg gatggtggtc cgggatgccg tataccgcgg tcgaggagat
      121 gtgcacgacg cgctccacgc cgttctcctt cgccgccttg aggacgttcc gggtcccgtc
      181 gatgtcggtg gagcggatat cctcggcgga atagaggggg agggcggccg cggtatgcac
      241 cacgatgtcc gtgcccttca tcgcgtctcg gagcgctccg tagtcgcgga tgtcgcccgt
      301 gacctcggta atgcggtccc gttcaggata gtcgaaggga agcaggtcga aggagacgac
      361 ctcctcgccc ttatccaaga ggtgccgcac gaggttgatg ccgaggaacc cggcgcctcc
      421 ggtgatgaaa tagcgggcca tgtgcctgag attgtatcaa aaaaggtacc gggccgcccc
      481 acgcgtggga cggcccgctc gcctcagatg aggccggtta gggcgaaggc gatgagggcc
      541 ggccgcgcct tccgcttctc gaagcggagg aatcgctcag gcgtcctgca ccagtcctgc
      601 gctcctggac gcactgttgg atcgtaggga actcggcgcg aagccccgtc gatcttcggc
      661 gtgagcccgt agtagcgcgc gagcgcggct acgcgctgca catgatcgag gtggccgatc
      721 aggccgacgc ggatctcctc cttttccggg aattgctcgc ggagcgtatc cgcgatccac
      781 ttcatgagcc ggtcggtacg gacccattgc ccgcttccat cgcttccggg gtattcagcg
      841 agcaccatgc agtccgaata ctgccgaatc aggttggtgt gctcccactg accgatgaac
      901 ggcaccccga gcgcctccat gcgctcaatt gcgaccttag cgatggccag gttgcttgca
      961 tccggatcgt cgcccttcag gtagctgttg aacggcactg ccgcgtacac gtcgaggtca
     1021 gtgagatcga gcgccaggtt ccgggtgcgg atcggttggc ataggtcccg gatgagacgg
     1081 gcacgtgcgg ataatgaggg catgggcccc tcctctggtt gcggtctgac gcaatagaac
     1141 tacgaaaaag acacagagtc aaatcttagg gaaggtagaa gatcgcggca agcacaagcg
     1201 tccaaaacac ggtcaggccg aaggtccacc cgtccttgaa taccaacagt tcaggagact
     1261 cggcacgcgc gggatcggtg gtcataatgt tcagcatccg gaagaagacg gtcgcgacga
     1321 ggatggtgga ataggcagcg gcggtcgacg ggtgcgtgaa gatggcccag agtccgtaga
     1381 aggcgatcgc gaggaccgca gaacttatga gcagatatcc gagcatctgt tccgaatagc
     1441 cgtcgagcac cttccggcgt accgtatgcg taaattctcc ctggcgcttg ccggagacga
     1501 ggaagagcgc gacgaagaag gtcgcgagca cgatccaggg cgagagcgcg atcgccgcgg
     1561 ccgcgccgcc cgcgaccacc cggagcacgt agaagcttgc gacgagcgag atgtcgatga
     1621 ccggcacgtg cttcagcgag aaggtatagg cgaggttgag gagcgcgtag acaaggaaga
     1681 ccgggatcgc ggtcgggatg aggaagagga ggatgcaggt tacgagcgtg agcgcgatcg
     1741 caagcaggat cgcagaaagc ttgctgaccg tcccggacgg gagcggccga cgcgccttca
     1801 ccgggtgctc ccgatcggcc tcgagatccc gcaggtcatt caccacgtac accgcgctcg
     1861 caagcgtcga gaacgcgacg aagcagacaa gcgccgcgat gaacgccgac gggacaaaga
     1921 acgcgcctgc gaagaagatg ggcgcgaaca cgagggagtt ctttacccac tgctgcgggc
     1981 gcatgagttg gaggtagggc gcggcgtgcc agacgaggtc atcgagaagg taggcgaagc
     2041 gggcgaaggc gtcatccgct tcgccgcgca gccgcaccac cttgatgaat tccgcgccga
     2101 tgagccgggc gaagatgccg tcggtgagga acttgtcacc gatcacgacc acgcgctccc
     2161 gccgctcgat ggagcggacc gcggcatgcg gcaggggctt cctaagctgc gggagcgctt
     2221 ggacgcctgc ggctttcgcg agcgccgcgg cccggaacgg atcgccgttt gagctcaggt
     2281 acacgtcgtt gtgcgcagcg agcgcccgga tcttctcgag cgcgtcgggc gcgatgtcac
     2341 tttgcttgtc gccgaccagc gttccgtcga tatcgaggaa gacggtcgcg tcccgaaggt
     2401 cgtgggtgag ttcggagaaa cgcatggagg ttagcctgcg cggtacacga ccagcgtgag
     2461 cccgatgacg atgagcgcgt agccgccgag ctggagggtc gtgatgcctt ccctcagccc
     2521 gaagaaggcg tacccgttga tgatgaggaa gctcatggcc accatgaccg gatacgcgat
     2581 cgagagcggc agggcgcgca aggcgaggaa atagaatatg acgttcgcgg cgaagaggaa
     2641 gagcccgagc aggaactgcc agttcgacac gatgagagta ggcacgttcc cagacaggga
     2701 gagccccttc acggacccga gcttgagaag cacgttcgcg acgccgttca agaggaaggc
     2761 cgcgaagagg tagatatagg ccataccgta actatcatac tgcccggctc atgggtcaag
     2821 gaaggggcaa cgtctctcgc ggggcagagg tttccgtata gtatagaggc atccccatgc
     2881 tattcaccct gaagaagctt gcgctccttg cgctcgtcgc gctccatggc cccgcgaggc
     2941 gctgcccgga gcggccgcag cgggtcatcg tcgtgcagca ggcgaagctt ggcgacatga
     3001 tctgcacgac gcccgtgttc gccgcgctga agcgtaccta taccgacctc catctcgcgg
     3061 tcatgggcaa tcgcgtgaat gaggacgtgc ttcgcggcaa cccgcacgtc gaccggtacc
     3121 tcgtcttcac cgacttccgt tcggcgctga aggaagtgcg caagggcggt ttcgataccg
     3181 gcatcgtcac cggccccgac ttcgtctcga tcgcgctcct catcctcgcg ggcgtcggct
     3241 gcgtcatcgc gccctccatc gagaaggggg tgaccccgct cgcaaactcg ctctatcgat
     3301 ctcttctccg ctttgtcgtt agccttccgc atgaggaagg gacgtatgcg cctggccagt
     3361 acctccggct tcttgagccg ctcggaatac acacgacaga taccacgaag gtccttgcat
     3421 atacgccttc ggcgcatgcg accgctgcga cctacctcaa gtccatcggc gagcgcccga
     3481 agctcctcgt cggcatgacg ccctcagcgg ggaatacggt gaaggagtgg ccgcccgagc
     3541 ggttcgccgc gatcgccgat catctcatct ctacgtacgg ggcgaaggtc attgtgctcg
     3601 gcacgaaggc ggagcgttcg aagatcgacg cgatgctcga gcaggtgaag gagaagcagg
     3661 acgtcatcga tgcgggcgga cgcttcgaca tcgaagaatt gaaagcgctc atcgccgcac
     3721 tcgatctctt cattgcggca gatacgggcc ctatgtatat cgccgaggcg ttcaaggtcc
     3781 ccacggtgga tatcgtaggg ccggtcgacg agcgcatcca gccgccgcag ggcccgctgc
     3841 atcgggtggt catcccgcct cgatccagac ctgcgcaggt aacggtgctg aacgcgaagt
     3901 ggtacgaccg tgaggaagcg cgcaagcagg ccgaagcaac gacggtcgcg gcggtcatcc
     3961 gcgaagtcga cgacctcatc gcttccatga aataatcgcg gtatgcccgg catctaccag
     4021 attgaacgcg cgctgctcga gaagcaggtc cgccgctatt cttcgtacat taaaggtcgc
     4081 gtgctggacg ccggcagcgg cagcatgaac cggtacggga agctcttcga cgcggacgaa
     4141 tacgtcaggc tcgacgtcgc gcccggcagc aacgtggacg tggtcggtag catcgaggag
     4201 atgccgttta cggacgccga gttcgatgcc gtcgtctcga cgcaggtcct cgagcatgtt
     4261 ccgcacccgc acctggcggc caaggagatc ttccgggtgc tgcgtcccgg cggccgtgcg
     4321 ctcatcacgg tgccccagtg gaacgagctc catgaggagc cgcatgacta ctggcggtat
     4381 acctccttcg ggctcgcgaa cctcttcacc gaagccggct tcaccgtcct cgcgtccgag
     4441 cagcgcggcg gattcttcgc tgcccgcgcg cagatgggga tgcggtacct catcgatcgg
     4501 ttcagcctgt accagcactg gtggggcagg gccttccatt acgccttccg cgtctggggt
     4561 gctgttgcgc tcgcgctcga tcgcgcagac cgaagcgcgg cgaatcgcaa gcacgcgatc
     4621 ggctggatct tcgtgttcga gaagccgcta tgaagatcgc gatactccag gacgatttcc
     4681 ccccgcacca ccagggcggc tcgggcgtga tcgcccatgc gctctccgtc gagcttgcgc
     4741 ggcaggggca tgaggtcatc gtcatctctg ccgccgcgga ccgacagcat gccggtacct
     4801 tcgtacgaga cggtctcacc gttcata
//
LOCUS       DASPKG010000074         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_646991, whole genome shotgun sequence.
ACCESSION   DASPKG010000074 DASPKG010000000
VERSION     DASPKG010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646991"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VEA36_03035"
     CDS             <1..676
                     /locus_tag="VEA36_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HYF29311.1"
                     /translation="VGKSALDIDGFGAKTVRLLMAHGLLNSYDDIFRLTYDQLMALPG
                     FQDVSAKKLLAAIEDARSVPLNRLLVGLSIDHVGEETALLLARHFNTLKRLKDAKRRD
                     LEAVPGIGPVVTQSIASWFEDAENHALLERLDEQLTVERVAMPDRDGAFEGLTIVVTG
                     TLPTLSRERAEAMIKRAGGKVSGMVSRRTSFVVAGEKAGAKLAKANELGIPVIDEREF
                     KRRLGV"
     gene            690..980
                     /locus_tag="VEA36_03040"
     CDS             690..980
                     /locus_tag="VEA36_03040"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HYF29312.1"
                     /translation="MATPEDVKKLAELARIAVPDTELDAFTKEFDGVLAYIGRLEELS
                     LAHAAPAAGALRNVLREDAAPHEPGRYTEALAAQFPKRAGDALSVQQIISYD"
     gene            984..2411
                     /gene="gatA"
                     /locus_tag="VEA36_03045"
     CDS             984..2411
                     /gene="gatA"
                     /locus_tag="VEA36_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HYF29313.1"
                     /translation="MAPNELTITEAARRLRAKELTVRGLWDACHAAAEAKNGELNAYL
                     ELFDADEGAIAAAQRRIDEEGDAAPMLCGIPIAMKDNILIEGRIASAASKILEHYRAT
                     YDATVTRKLKEAGALFTGRTNMDEFAMGSSTEHSAFGKVLNPLDTSRVPGGSSGGSAA
                     AVAAGMALGALGTDTGGSIRQPASHCGLVGMKPTYGAVSRSGIIALGSSLDQAGPLAA
                     SVADAKALFEAIRGKDPLDATSIDADTYPKREAGERLQIGVPAHLLEGVDPDVRANFE
                     AKLDALRERGHTITEVSLPTSSYALPAYYVIMPAEASTNLARFDGIRYGRSAAGRDLL
                     QSYVRARTEGFGPETKRRILLGTFVLSSGYIDAYYRTADAARRVLVDEYNEAFRAVDV
                     IVFPTTPSPAFKFGEKSDPVSMYLEDIFTVTANLTGMPAISVPMGNVERDGTALPTGI
                     HFTAPHGQDDVLFRIGADAVGETLY"
     gene            2418..>2806
                     /locus_tag="VEA36_03050"
     CDS             2418..>2806
                     /locus_tag="VEA36_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29314.1"
                     /translation="MEGDSSIHFLNLSYFFRVLYDLLTGGKVVSTGDVWSDLLLLATQ
                     FWILLTVFGFLISFLALAVLVHATVRMMEAKRVEAPKYATLHPTVAETRRDHNRWNHI
                     MQLIESPHENDWRQAIIEADIMLDDLLS"
BASE COUNT          479 a          949 c          933 g          445 t
ORIGIN      
        1 cgtcgggaag tccgcgctcg acatcgatgg gttcggggct aagaccgtgc ggctcctcat
       61 ggcgcatggg ctcctgaact cgtacgacga tatcttccgg ctcacgtacg accagctcat
      121 ggcgctcccg ggcttccagg acgtctcggc gaagaagctc ctcgccgcga tcgaggacgc
      181 gcgctccgtg cccctgaacc ggctcctcgt cgggctctct atcgatcatg tgggggaaga
      241 gaccgcgctc ctcctggccc ggcatttcaa tacgctcaag cgcttgaagg acgccaagcg
      301 ccgggacctc gaggcggtgc ccggcatcgg gccggtcgtc acgcagtcca tcgcctcctg
      361 gttcgaggat gcggagaacc atgcgctcct cgagcggctc gatgagcagc tcacggtgga
      421 gcgcgtcgcg atgcccgacc gcgatggcgc gttcgagggc ctcaccatcg tcgtgacggg
      481 gacgctcccg acgctgtcac gcgagcgcgc cgaggcgatg atcaagcgcg cgggcggcaa
      541 ggtctcgggc atggtgtccc gcaggacgag cttcgtcgtc gcgggcgaga aggcaggcgc
      601 caagctcgcg aaggccaacg agctcgggat acccgtcatc gacgagcgcg agttcaagcg
      661 gcgcctcggc gtgtgataca gtggcggcaa tggccacccc ggaggacgtc aagaaactcg
      721 ccgagcttgc ccggatagcg gtcccggaca cggagctcga tgccttcacg aaggaattcg
      781 atggcgtgct cgcgtacatc gggcggctcg aggagctctc gcttgcgcat gcggcgcctg
      841 cggcaggcgc gctgcgtaac gtgctgcggg aggacgccgc accgcacgag ccggggcggt
      901 ataccgaagc gctcgccgcg cagttcccga agcgcgcggg cgatgcgctc tcggtgcagc
      961 agatcatctc ctacgactag accatggcgc cgaacgaact gaccatcacc gaggcggcgc
     1021 gaaggctccg cgccaaggag ctcacggtcc gcgggctctg ggatgcctgc cacgctgccg
     1081 ccgaggcgaa gaacggggaa ttgaacgcct atctcgagct cttcgacgcg gacgagggag
     1141 cgatcgctgc ggcccagcgg cgcatcgacg aggaggggga cgcggcgccg atgctctgcg
     1201 gcatcccgat cgcgatgaag gacaacattt tgatagaagg gcgtatcgcg agcgcggcct
     1261 cgaagatcct cgagcattac cgcgcgacct acgatgcgac cgtcacgcgg aagctcaagg
     1321 aggcgggagc cctgttcacg ggacgcacca acatggacga gttcgccatg ggcagctcga
     1381 ccgagcactc ggccttcggg aaggtcttga acccgctcga tacctcgcgg gtaccgggcg
     1441 gctcttcggg gggctctgcg gccgcggtcg ccgcggggat ggcgcttggc gcgcttggca
     1501 ccgacacggg cggctcgatc cgccagcccg cctcccactg cgggctcgtc ggcatgaagc
     1561 cgacctatgg cgcggtgtcc cggagcggca tcatcgcgct cgggagcagc ctcgaccagg
     1621 cggggccact ggccgcgtcg gtggcggacg ccaaggcgct cttcgaagcc atccgcggca
     1681 aggacccgct cgatgcgacc tccatagacg cggacacgta cccgaagcgg gaagcggggg
     1741 agcggctgca gatcggggtg ccggcgcatc tccttgaagg cgtcgacccg gatgtgcgag
     1801 cgaacttcga ggcgaagctc gacgcgctcc gggagcgcgg ccacacgatt accgaggtct
     1861 cgctcccgac gagctcctac gcgctcccgg cgtactacgt catcatgccg gcggaggcct
     1921 cgacgaacct cgcccgcttc gacggcatcc gctacggcag gagcgccgcg gggcgagatc
     1981 tcctccaatc gtacgtccgt gcacggaccg aagggttcgg tcccgagacc aagcgccgca
     2041 tcctgcttgg caccttcgtg ctctcctccg gctacatcga cgcgtactac cgcacggcgg
     2101 acgcggctcg ccgggtgctc gtcgatgagt acaacgaggc gttcagggcc gtggacgtga
     2161 tcgtcttccc gacgaccccg tcccccgcct tcaagttcgg ggagaagagc gatccggtgt
     2221 ccatgtacct cgaggacatc ttcacggtca ccgcgaacct gaccggcatg cccgcgatct
     2281 cggtgccgat ggggaacgtg gagcgcgacg gcaccgcgct tccgaccggc atccacttca
     2341 cggcgcccca tggccaggac gacgtattgt tcaggatcgg cgcggacgcc gtcggcgaga
     2401 cgctatacta gaggctaatg gaaggcgact cctcgatcca tttcctcaac ctttcgtact
     2461 tcttccgcgt cctctacgac ctcctgaccg ggggcaaggt cgtgtcgacg ggcgatgtgt
     2521 ggtcggatct cctgctgctc gcgacccagt tctggatcct tcttaccgtc ttcggattcc
     2581 tcatctcctt cctggcgctc gcggtcctgg tccacgcgac cgtgcgcatg atggaggcca
     2641 agcgcgtcga agcgcccaag tacgcgacgc tccatccgac ggtcgcggaa acccgccgcg
     2701 accacaaccg ctggaaccac atcatgcagc tcatcgagag cccgcatgag aacgattggc
     2761 gccaggccat catcgaggcg gatatcatgc tcgacgatct cctctc
//
LOCUS       DASPKG010000075         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1871201, whole genome shotgun sequence.
ACCESSION   DASPKG010000075 DASPKG010000000
VERSION     DASPKG010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871201"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VEA36_03055"
     CDS             <1..442
                     /locus_tag="VEA36_03055"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYF29315.1"
                     /translation="NYYQNKNVIDRLTNAIKTMEKAGLFVVILFAGASVIIALATIRL
                     AIYTAKDEIAVMRLVGASNMYIRGPFIVAGILSGVFAAIIALILFYPATWYAGQALST
                     WLGGFNLFEYYTDNFVMVFGILVGSGVFIGALASWLAVRRYLRV"
     gene            445..2100
                     /locus_tag="VEA36_03060"
     CDS             445..2100
                     /locus_tag="VEA36_03060"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HYF29316.1"
                     /translation="MAREGHKYIFVLGGVMSGVGKGIATSSMGLILKERGYEVNLVKV
                     DPYLNVDAGTMNPTEHGEVFVLTSGLETDQDMGNYERFLGRDLGTQDYMTSGMVYQSV
                     INRERALEYGGRCVEAIPHVRDEILRRIEAAADHNGSRISVVEIGGTVGDFQNALFIE
                     AARVMKLKHPNDVLFVMVSYMPVPSTLGEMKTKPTQSAVRELNSYGVQPDFILARSKQ
                     PMDDKRKEKLAISCNVLPERVISAPDVESIYDVPLNFEKDNMGDLLLDALSLSKDQAA
                     DFSEWKEFVDRTKIDRPVVKIGIVGKYFDTGDYVLSDAYLSVIEAIKFSAAAAGVKAV
                     VEWVNAKHIESATDAEKLEQYDGIIVPGGFGETGIEGKIRAAEYARTHRIPYFGLCYG
                     MHMMVIEYARNVLGLAGAHTVEIDPSSPHPVVDVMPDQKDLIAEGKYGGTMRLGTYPA
                     HLEEGTLAKEAYGMDVVHERHRHRYEINPEYIDRLVGKGLVFSGKSPDGVLMEIAELP
                     KKEHPFYLGCQFHPELQARPLSPHPLFTKFLEAAHKRSEFKDA"
     gene            complement(2170..>2369)
                     /locus_tag="VEA36_03065"
     CDS             complement(2170..>2369)
                     /locus_tag="VEA36_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29317.1"
                     /translation="LSHPFPNITIDVVEAGGYCRMRDQTTGQPQKRVALLADLVFCPL
                     SWAEQRCFRRFLDQCELQAAA"
BASE COUNT          454 a          728 c          769 g          418 t
ORIGIN      
        1 caactactac cagaacaaga acgtgatcga tcggctcacg aacgccatca agaccatgga
       61 gaaggcgggc ctgttcgtgg tgatcctctt tgcgggcgct tccgtcatca tcgcactcgc
      121 gaccatccgg ctcgccatct ataccgccaa ggacgagatc gcggtgatgc ggctggtcgg
      181 cgcctccaac atgtacatcc gcgggccctt catcgtggcc ggcatcctct cgggcgtgtt
      241 cgcggccatc atcgcgctca tcctcttcta tcccgcgacc tggtacgcgg ggcaggctct
      301 ctctacctgg ctcggcggct tcaacctctt cgagtactac acggataact tcgtgatggt
      361 gttcgggatc ctggtgggct ccggcgtctt catcggggcg ctcgcgtcct ggttagcggt
      421 acggcggtac cttcgcgtgt aggtatggcg agggaaggac acaagtacat cttcgtcttg
      481 ggcggcgtga tgagcggcgt cggcaagggc atcgcgacct cctcgatggg gctcatcctc
      541 aaggagcgcg ggtacgaggt gaacctcgtg aaggtggacc cgtacttgaa cgtcgacgcc
      601 ggcaccatga acccgaccga gcatggcgag gtcttcgtgc tgacctccgg gctcgagacg
      661 gaccaggaca tgggcaacta cgagcgcttc ctcgggcggg acctcggcac ccaggactac
      721 atgacctccg gcatggtgta ccagtcggtc atcaaccggg agcgggcgct cgagtacggc
      781 gggcgctgcg tcgaggccat cccgcacgtg cgcgatgaga tcctgcgccg catcgaggcg
      841 gctgcggacc ataacggcag ccgcatctcg gtcgtcgaga tcggcggcac ggtgggggac
      901 ttccagaacg cgctcttcat cgaagcggcc cgcgtgatga agctgaagca cccgaacgat
      961 gtcctgttcg tcatggtgtc atacatgccg gtcccctcga ccttggggga gatgaagacc
     1021 aagcccacgc aaagcgcggt gcgcgagctc aactcctacg gcgtccagcc tgatttcata
     1081 ctcgcccgga gcaagcagcc gatggacgac aagcgcaagg agaagctcgc catctcctgc
     1141 aatgtgcttc ccgagcgggt gatctccgct ccggacgtcg agagcatcta tgacgtcccc
     1201 ttgaacttcg agaaggacaa catgggcgat ctgctccttg atgcgctctc gctttccaag
     1261 gaccaggcag ccgatttctc ggagtggaag gagttcgtgg accgcacgaa gatcgaccgg
     1321 cctgtcgtga agatcggcat cgtggggaag tatttcgata cgggcgacta cgtcctctcg
     1381 gacgcgtacc tgtcggtcat cgaagcgatc aagttctccg cagccgcggc cggggtgaag
     1441 gccgtcgtcg aatgggtgaa cgccaagcac atcgagagcg cgacggacgc ggagaagctc
     1501 gagcagtacg acggcatcat cgtgccgggc gggttcgggg agaccggcat cgaggggaag
     1561 attagggcgg ccgagtacgc ccgcacgcac cgcatcccgt acttcgggct ctgctacggc
     1621 atgcacatga tggtcatcga gtacgcgagg aacgtgcttg gccttgcggg cgcgcacacg
     1681 gtcgagatcg acccaagctc gccgcatccg gtcgtggacg tgatgccgga ccagaaggac
     1741 ctcatcgccg aagggaagta cggcggcacc atgcggctcg gcacgtatcc ggcgcatctc
     1801 gaggagggca cgctcgccaa ggaagcatac ggcatggacg tggtacacga gcgccaccgc
     1861 caccgctacg agatcaatcc ggaatacatc gatcggctcg tcggcaaggg gctcgtcttc
     1921 tcggggaagt cgccggacgg cgtccttatg gagattgccg agcttccgaa gaaggagcat
     1981 ccgttctacc tcggctgcca gttccatccg gagctgcagg cgcgccccct gtcaccgcac
     2041 ccgttattta cgaaattcct cgaggcggcg cacaagcgct ccgagttcaa ggatgcataa
     2101 gaaaaggccg ccccgaagga cggcctgaaa gttctgtagg gcccgcgcgg gccgaggttc
     2161 atttgtgtgt caggcggcgg cctggagctc gcattggtca aggaagcgcc ggaagcagcg
     2221 ttgctcggcc cagctgagag ggcagaacac gagatccgca agcagcgcga cgcgcttctg
     2281 cggctgcccg gtcgtctgat cgcgcatgcg gcagtacccg ccggcctcga ccacatcgat
     2341 ggtgatgttc gggaagggat ggctcagca
//
LOCUS       DASPKG010000076         5079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_245905, whole genome shotgun sequence.
ACCESSION   DASPKG010000076 DASPKG010000000
VERSION     DASPKG010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245905"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1165)
                     /locus_tag="VEA36_03070"
     CDS             complement(257..1165)
                     /locus_tag="VEA36_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29318.1"
                     /translation="MRQALASLVLVIVGLIASPAAAGIMSDTAKCTTWVRENVHYFKD
                     RIGVTEAEAYSIAEGFRYQMPDGSWRTLTPRGASFWGGCYAYVRDGGDLSKITATSTA
                     TAKAPTAAGTVTEATPALPETTTPAEQPAGVRRREPELAEAPAPVPPAAPVEKTLEQP
                     VEKSAPKADPAPAGSPPVDGTTPLERPATDNAIGNWLANNLTFAVLGVVLVAALGIGF
                     AAGYGEHLERRRSKELPYQEAIDRGRQHVIATANAKLPERVRSWMEREAEEPLEEENP
                     GGDPAHGLRFRDAITDEPIPKDAVRH"
     gene            complement(1308..1586)
                     /locus_tag="VEA36_03075"
     CDS             complement(1308..1586)
                     /locus_tag="VEA36_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HYF29319.1"
                     /translation="MASTKAGGSAKNLNTSNPKYLGVKLADGAPARPGMVIVRQRGTK
                     IEAGRNVKVGKDHTLYAMSTGKVVFREKRKTNFDGRVVRKKVVEVLAA"
     gene            1610..1999
                     /locus_tag="VEA36_03080"
     CDS             1610..1999
                     /locus_tag="VEA36_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HYF29320.1"
                     /translation="MSIPERIGGSKAGGRGAADYLIILVILLAVTAAFGLGVLAGREM
                     GEGKGDRLWIEELSTEARAQPAAVAAPAAAAATSGTYVASKSGTKYHLPSCSGAKRIK
                     EENKVWFATKEAAEAAGYAPAANCPGL"
     gene            1996..2589
                     /locus_tag="VEA36_03085"
     CDS             1996..2589
                     /locus_tag="VEA36_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYF29321.1"
                     /translation="MIVLDVESTGLIPERCSILSLGAVDLDDPTNQFYDECRIWDGAH
                     ITDEALAINGFSREEIQDPAKKPEAELIASFIAWATDKPLDRTLAAQNVSFDLEFVQA
                     AAHRAGLESPFGKRTLDVHTLVWMHMTSRGATPPLANRHSALNLDFALKYVGLPEEPK
                     PHNGLTGALCHAEVIARVAYNKKVFPEFSTYDIPWHE"
     gene            2582..3406
                     /locus_tag="VEA36_03090"
     CDS             2582..3406
                     /locus_tag="VEA36_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HYF29322.1"
                     /translation="MNENQLTELSISTPEGELVIPPEAIGAGEAGARDRAKDTAMAAV
                     LTEAIQHGKGSVPEDPAPLVCKPRTIESWRIFKIMAEFVDGFEILKRYGLTATFFGTA
                     RESFSPEVYAAATELAGKLAKAGFSIITGGSAGVMQAANKGAYDAGGSSIGLNIRLSD
                     HQPLNGYLTDSLVFDHFFVRKVMLAFASEVYIYFPGGFGTLDEFTEMVTLIQTKKIKP
                     IPIVLYGREYWSPFIDLFRNHLLAKYHAIDEADLELFHLVDSVDEAFEYITQNAKC"
     gene            complement(4347..4916)
                     /locus_tag="VEA36_03095"
     CDS             complement(4347..4916)
                     /locus_tag="VEA36_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYF29323.1"
                     /translation="MNSQACADLPEKPKIVAFGDSLVTGYGTTDNGDFVSILSSRLEV
                     PITNLGRDGETSASARERIADVVRERPDVVIILLGGNDALQKVPVAETRENLDAILTT
                     LAEISSVKINIVLVGVLGSPLNDPYRAMFEELAEKHQVEYVPNILSGIFGNRDLMSDQ
                     IHPNNAGHARMAERIYPAVLAACESVMGF"
BASE COUNT          949 a         1594 c         1582 g          954 t
ORIGIN      
        1 gctcgccgag gcggcgcagc tccccgagga ggtcatccgc atcgaacggc ccttcaagga
       61 actcggcacc ttcgaggtcc cggtcgcgta cggcgaccag ttcgggaaat tcaccgtcga
      121 gatcgtcgcg gagtagagaa gcgcgaaaat ggaaaggcgc cggctcacgg agcggcgcct
      181 tcttccagcc tcgaacgcgt gcgaatagtg ggcccaagca cgcttacgga cccgtaggca
      241 gcttgcgctg cgatgttcag tgccgtaccg cgtccttcgg aataggctcg tcggtgatgg
      301 cgtcgcggaa acgcagcccg tgggccggat ccccgccagg gttctcctcc tccagtggtt
      361 cttccgcctc cctctccatc cagcttcgaa cgcgttccgg aagcttggcg ttggcggtgg
      421 cgatgacgtg ctggcgccct cgatcgatcg cttcctggta gggaagctcc ttcgagcgcc
      481 gtcgctccaa gtgctctccg tagccggcgg cgaacccgat gccgagggcg gcaacgagaa
      541 cgacgccgag gaccgcgaag gtgaggttgt tcgcgagcca gttgccgatc gcgttgtccg
      601 tagcggggcg ctcgagcggg gtggtgccgt cgaccggagg tgatccggcg ggcgccgggt
      661 ccgccttcgg agccgacttc tcgaccggct gctccagggt cttctcgacc ggagccgcag
      721 gcggcaccgg agcgggcgct tcggcaagtt ccggctcccg acggcgaacg ccggccggtt
      781 gctcggcggg cgtcgtggtc tcgggaagcg cgggagtcgc ctcggtgacg gtcccggctg
      841 cagtcggcgc cttggcggta gcggtggacg tcgcggtgat cttgctcagg tccccgccgt
      901 ctcgcacgta ggcgtagcag ccgccccaga agctcgcgcc acggggggtg agcgtgcgcc
      961 agctcccatc gggcatctgg tagcggaatc cttccgcgat actataggcc tccgcctcgg
     1021 tcacgccgat gcggtccttg aagtagtgaa cgttctcgcg cacccacgtg gtgcacttgg
     1081 cggtgtcgct catgatgcct gccgcggcgg gcgaggcgat gaggccgacg atgacaagca
     1141 cgaggctcgc cagcgcctgt cgcatgaacc tgcacatctg tttcaaatcc tgaggctgga
     1201 atggaacgac catttgggcg gatgcccggg tctgcctcat atatcaccat gatttatata
     1261 caaaagcaag accccggccg gggccggggt ccagcgagga gggaagatta cgcagcgagg
     1321 acctcaacga ccttcttcct gaccacgcgg ccgtcgaagt tggtcttgcg cttctcgcgg
     1381 aagacgacct tgccggtgct catggcgtag agcgtgtggt ccttgccgac cttcacgttc
     1441 cggcccgcct cgatcttcgt gccgcgctgg cggacgatga ccatgcccgg gcgcgccggg
     1501 gcgccatcgg cgagcttcac cccaaggtac ttggggtttg aggtattcag gttctttgct
     1561 gatccgccgg ctttagtact tgccatgttg ctataatctt tacgtagcga tgagtatacc
     1621 agagcgaatc ggcgggagca aggcaggcgg ccgcggcgcg gccgattacc tcatcatcct
     1681 ggtgatcctc cttgcggtca ccgccgcctt cgggctcggc gtcctcgccg gccgggagat
     1741 gggggaaggg aagggggatc gcctctggat cgaggagctc tcgacggagg cgcgggcgca
     1801 gccggcagcg gtcgcagccc cagccgccgc ggcggccacg agcgggacct acgtcgcctc
     1861 gaagagcggc acgaagtacc atctcccgag ctgcagcggc gccaagcgca tcaaggagga
     1921 gaacaaggtg tggttcgcaa ccaaagaagc ggcagaggcc gcaggctatg ctcccgccgc
     1981 taattgtccc gggctatgat cgtgctcgac gtcgaaagca ccggcctcat ccccgagcgc
     2041 tgctccatcc tctcccttgg cgcggtcgac ctcgacgacc ccaccaatca gttctacgac
     2101 gagtgccgca tctgggacgg tgcgcatatc acggacgagg cgctcgccat caatggattc
     2161 agcagggagg agatacagga ccccgcgaag aagcccgagg ccgagctcat cgcctccttc
     2221 atcgcctggg cgaccgacaa gccgctcgat cggacgctcg ccgcccagaa cgtctccttc
     2281 gatctcgagt tcgtgcaggc agccgcgcac cgggcaggcc tcgaatcccc cttcggcaag
     2341 cgcacgctcg acgtccatac gctcgtctgg atgcatatga cgagccgggg agcgaccccg
     2401 ccgctcgcga accgccactc ggccctgaat ctcgacttcg ccttgaagta cgtcgggctg
     2461 ccggaggaac ccaagcctca taacggactc accggggcgt tatgccacgc ggaagtgatc
     2521 gcgcgcgtcg cgtataataa aaaagtattc cccgaattta gcacctacga catcccctgg
     2581 catgaatgag aaccaactca ccgaactctc gatctcgacc cccgagggcg agctcgtgat
     2641 cccgccggag gccatcgggg caggggaggc cggcgcgcgc gacagggcca aggacaccgc
     2701 gatggccgcg gtcctgaccg aggccatcca gcacgggaag gggagcgtgc cggaagatcc
     2761 cgcgcccttg gtctgcaagc cccgcaccat tgagagctgg cgcatcttca agatcatggc
     2821 cgagttcgtg gacggcttcg agatattgaa gcgctacggc ctcaccgcga ccttcttcgg
     2881 caccgcgcgc gagtccttca gtcccgaggt atatgccgcg gcgaccgagc ttgccgggaa
     2941 gctcgccaag gccggtttct ccatcatcac gggtggatct gcgggcgtga tgcaggcggc
     3001 caataagggg gcgtacgacg ccggcggctc ctcgatcggc ctcaacatcc gcctgagtga
     3061 tcaccagccc ctgaatgggt acctcaccga ctcgctcgtc ttcgatcact tcttcgtccg
     3121 caaggtgatg ctcgcattcg cctctgaggt ctacatctac ttcccggggg ggttcggcac
     3181 cctcgatgag ttcacggaaa tggtgacctt gatccagacg aagaagatca agccgatccc
     3241 gatcgtgctc tacggcaggg aatactggtc gccattcatc gacctattcc gcaaccatct
     3301 cctcgcgaag taccacgcca tcgacgaggc ggacctcgaa ctcttccatt tagtggattc
     3361 ggttgacgag gcgttcgaat acataacgca aaacgcgaaa tgctagccgt ttgacacgcc
     3421 cgggcactgg gcgtacggtg acccgagcaa tggccgttgc gaaaggaggg tatggccatg
     3481 acccagctcc tcaagcgtcc gcgtacggcg gacgagatac tcgctaaccc cagctgcctc
     3541 ctcagtctgc gagcggaaga gatcgtagaa gtcgaggcaa tgccacccag caggtcctac
     3601 cgtcctctgc ggttgggggc gcattggctc cgcctcgcgc ttctggtggt cgcggcattc
     3661 gtaatcaccg gtcatttctg agagggtagg ggacagggaa gagagggacc acgttggggg
     3721 tcccttttcc tatttcaaat tcctttctcc cccaccgccc ttcttcagcc gcatcgggga
     3781 aggggcggaa atatttgaga tatatttctg cacgtataag acggcgacaa ggtcgggcag
     3841 gaccggagcc gatagcggta ccccaatagg ggatagggga cccggcttta aggtcgggcc
     3901 aggggctccg ggctcaacat ctgtgaactt cgcacaatat atcttttgcg aatgataagc
     3961 aaacgcaatg ccgaggggct gggcttgggt ctctcttcaa cttggtgcac ttccctagcc
     4021 cgctagagcc tctgcaggtg ctgtattgca aagtatccgt gcacttcccc ctggggcttg
     4081 aatttcctga ctatcctctc tcggacagat tccacgctga tatcggggaa gtaccacttc
     4141 cctctcctcg ggtatccgag atgagggagt ccgagatgaa atatgcaaag agtgggccgt
     4201 aatttcggca ggggacttcc ctacttcttc ttcagtgatc cgggccagca gatgggacag
     4261 ggaccgactc cttggtactt atggccacga ctgcagacat tttgtttgac tgttgcgcca
     4321 tagccggcag ggtagctcag gccgaattaa aaccccatga cgctctcaca ggctgcgagg
     4381 actgccggat agatgcgctc ggccatccgt gcatgccccg cattgttcgg atggatctgg
     4441 tcgctcatca aatcgcggtt gccgaatatg cctgagagga tattcgggac gtactcaact
     4501 tggtgctttt cagccaactc ctcgaacatg gcccgatagg gatcattcag cggactgccg
     4561 agcacaccga ccagcacgat gttgatcttc acggacgaga tctccgcaag cgtggtgagg
     4621 atggcgtcga ggttctcccg cgtctccgct acaggcacct tctggagcgc gtcgttgccg
     4681 ccgagaagga tgatgacgac gtcggggcgc tcccggacga cgtccgcaat gcgctcccgt
     4741 gcagatgcgc tcgtctcgcc gtcccggccg aggttcgtga tcggcacctc gagcctgctc
     4801 gaaagtatgg aaacaaaatc cccgttgtcg gtcgtgccgt acccggtcac gagactatcg
     4861 ccgaacgcga cgatcttcgg cttctcggga aggtcagcgc atgcctgaga gttcatagaa
     4921 gagttagtac ggggaagcag gaagaccgca gcagtgccga gagcgactac cgcaagcgca
     4981 cctaccacta tccatcgctt catagatcgc tatagtcccg cggctacttc gcgaggtcaa
     5041 ggatctcgtc gatcctgatc tgcgccacga actccggca
//
LOCUS       DASPKG010000077         3509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1872776, whole genome shotgun sequence.
ACCESSION   DASPKG010000077 DASPKG010000000
VERSION     DASPKG010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872776"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VEA36_03100"
     CDS             <1..400
                     /locus_tag="VEA36_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYF29324.1"
                     /translation="RIADNFATMLQSGVPVVESVEITASVVGNARYEQVLKDVGEEVK
                     GGASISDALNRHEEIPGIMSAMVRVGEETGELGNILTTLAKFYRREVTNAVDTLVDLI
                     EPMMIVLLGLGVGVLLASVLIPIYNLAGSI"
     gene            501..1007
                     /locus_tag="VEA36_03105"
     CDS             501..1007
                     /locus_tag="VEA36_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYF29325.1"
                     /translation="MNLAPISVRRGHLKGFTLIELLVVIAIIGILSSVVLASLNTARN
                     KGADAAIKSNLSAARAEAEIYYDSAGNYQDPTTAATGVCGSVANNIMDSATAAGRVLS
                     STFTVTRDADGGATVVSCNDTTAGWAIQAPLKTLNGANTQYYCVDSSGAALESTTDMI
                     TAATDVTC"
     gene            1124..1603
                     /locus_tag="VEA36_03110"
     CDS             1124..1603
                     /locus_tag="VEA36_03110"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HYF29326.1"
                     /translation="MSSPAPMHHPCSRGFTLIELLVVIAIIGILASVVLASLNTARSR
                     SEDAAVRTHLASTRAQAELYYDVNGFSYEGVCDDAPPIGEVKTIYLVIANAIAVSGGT
                     GIGTGPTTATEGTCNDDVATWAAEAALKTGGFYCVDNLGNAVTSTTALLGDGEMVCE"
     gene            1605..2354
                     /locus_tag="VEA36_03115"
     CDS             1605..2354
                     /locus_tag="VEA36_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HYF29327.1"
                     /translation="MDAALLVLAILGAIFASFIGVLVERAHTGGSWVGDRSRCNSCSA
                     TLGLLDLVPIASWLSTYGRCGHCGVRLPATYLVSEIALAGLFALAYATQGLGLPLLIF
                     LLALCVLLYIVLYDLRHTVVPMQAALILLALSILFAYLTAFDAGAFGLTLMFAGVIGL
                     MFFLLYALGRGRLMGLGDSPIALSLSLLVGFSAALPGLIFSFWIGALCGILILVWRRG
                     GPRMGIEVPFVPFLAAGYLLAFFTQWNPLVL"
     gene            2333..3016
                     /locus_tag="VEA36_03120"
     CDS             2333..3016
                     /locus_tag="VEA36_03120"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYF29328.1"
                     /translation="MESPRPLAPRGFTLIEMIVVLVIIVFITAVAVTGQSAFNRSLVL
                     TDTAYTVAFSIREAQNLGLSSRAVGGVYDAGYGIQFSTQTPTAYVLFADTSPASPGID
                     PADNPYCRGHEARVSTDPEAHPGDCIYNGSAELVRTYTLNRGFTISQFCGTRVSDNTQ
                     ACSGTHFQTMHISFMRPSTNTRIFGRTIDGVQTALSDATIRLRAPDGGAFRCITVSQV
                     GQIAVGACP"
     gene            3013..>3509
                     /locus_tag="VEA36_03125"
     CDS             3013..>3509
                     /locus_tag="VEA36_03125"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HYF29329.1"
                     /translation="MRTPNISRGYSLVELIVAVGIFSMTTLIVTSVYLALVNLDRQAR
                     ATNQLAANLDFAMESMVRNIRTGRDYECPPPNCNLTFTDSEDVLTHYRENSDGTIGIC
                     TGDDALNCTDENALPLTDPQITVEDLRFYVPDDEYQPRVVIVAEGTMETNLGEEIRFS
                     IQSSAA"
BASE COUNT          597 a         1173 c         1059 g          680 t
ORIGIN      
        1 ccgcatcgcg gacaacttcg cgaccatgct ccagtccggc gtgccggtgg tggaatcggt
       61 cgagatcacc gcgagcgtgg tcgggaacgc gcgctacgag caggtcctga aggacgtggg
      121 agaggaggtc aagggcgggg cctccatttc cgacgccttg aatcgccacg aggagatccc
      181 ggggatcatg agcgccatgg tccgggtggg cgaggagacc ggcgagcttg ggaacatcct
      241 caccacgctc gcgaagttct accgccgcga ggtgacgaac gcggtggata cgctcgtcga
      301 cctcatcgag ccgatgatga tcgtgctcct tggccttggc gtcggcgtgc ttctcgcatc
      361 ggtcctcatc ccgatctaca acctcgcggg ctcgatatag ccgcgtttat ccacacgcag
      421 ggaaattcat tggattatct ctcgtacaat acgtatagcg gatatttacc cgtaaccgca
      481 taacctttca ttcaatcatt atgaatcttg cacctataag cgttcgccga ggccatctca
      541 agggtttcac cctgatcgag ctcctcgtcg tgatcgcgat catcggcatc ctctcgtcgg
      601 tggtgctcgc atctctcaac accgcgcgca acaagggtgc ggatgcggcc atcaagtcga
      661 acctctccgc ggcacgcgcc gaggcggaga tttactacga cagcgccggc aactaccagg
      721 atccgaccac cgcagctacc ggcgtgtgcg gcagcgttgc caacaacatc atggacagcg
      781 cgaccgcagc cggccgggtc ctcagctcga ccttcaccgt tacgcgtgac gctgatggcg
      841 gcgcgaccgt cgtttcctgc aacgacacga cggctggatg ggcgatccag gcgccgctca
      901 agacgctcaa cggcgctaac acccaatact attgcgtgga cagctcgggc gcggctcttg
      961 agagcacgac cgacatgatc acggcggcga ctgacgtcac ctgctaacga gcccctcggt
     1021 tcatacggaa aggaccccgc atggggtcct ttccgtttcc aggggctcct gtatatagcg
     1081 cgcgaaaggg caggcgccgg gtactatact ggagacattg ctcatgagtt ctcctgctcc
     1141 catgcatcat ccgtgctccc gcggcttcac cctgatcgag cttctcgtcg tgatcgcgat
     1201 catcggcatc ctcgcgtccg tggtgctcgc atcgctcaat accgcgcgca gccgcagcga
     1261 ggatgcagcc gtgcgcactc acctcgcgtc cacgcgcgca caggccgagc tctattacga
     1321 cgtcaacggc ttcagctacg agggtgtctg cgatgacgcg ccgccgatcg gcgaggtgaa
     1381 gacgatctac ctggtcatcg cgaacgcgat cgccgtgagc ggcggcaccg ggatcgggac
     1441 gggccccacg accgcgaccg agggaacctg caatgacgat gtggccacct gggcggccga
     1501 ggcggcgctg aagaccggcg gcttctactg cgtcgataac ctcgggaacg cggtgacctc
     1561 gacgaccgcg ctccttgggg acggggagat ggtctgcgaa tagcatggat gccgcgctcc
     1621 tcgtcctagc gatcctcggc gcgattttcg cgagcttcat cggagtcctc gtcgagcgcg
     1681 cgcataccgg cgggtcgtgg gtaggggacc ggtcgcggtg caattcctgc agcgcgacgc
     1741 tcgggctcct cgatctcgtg cccatcgcct cctggctctc gacgtacggc cgctgcgggc
     1801 actgcggggt gcggctgccg gcgacgtacc tggtgagcga gatcgcgctc gcggggctct
     1861 tcgcactggc gtacgcgacg caggggctcg gcctcccgct ccttatcttt ctgctcgcgc
     1921 tctgcgtgct cctctatatc gtgctctacg acctccggca caccgtcgtg ccgatgcagg
     1981 ccgcgctcat cctccttgcg ctcagcatcc tcttcgcgta cctcaccgcc ttcgatgcgg
     2041 gcgcgttcgg cctcacgctc atgttcgccg gggtgatcgg gctcatgttc ttcttgctct
     2101 acgcgctcgg tcgtggccgc ctcatgggcc tcggggacag ccccatcgcg ctgtcacttt
     2161 cgctgctcgt cgggttctcg gccgcgctcc ccggcctcat cttctcgttc tggatcggcg
     2221 cgttatgcgg aatccttata ttggtatggc ggcggggagg gcctagaatg ggtattgagg
     2281 tgcccttcgt cccgtttctt gcggccgggt acttgctcgc cttcttcacg caatggaatc
     2341 ccctcgtcct ctagcacccc gcggcttcac cctcatcgag atgatcgtgg tgctggtgat
     2401 catcgtgttc atcactgcag tcgcggtcac ggggcagagc gccttcaacc gctcgctcgt
     2461 cctgaccgat accgcctaca ccgtcgcgtt ctcgatccgg gaggcgcaga acctcgggct
     2521 ctcgagccgc gcggtcggcg gcgtctacga tgccgggtac gggatccagt tctcgaccca
     2581 gactccgacc gcctacgtcc tcttcgcgga tacatcgccc gcgtccccgg gcatcgatcc
     2641 ggccgataac ccgtactgcc gcgggcacga ggcgcgcgta tccacggacc ccgaagcgca
     2701 tccgggcgac tgcatctaca acggctccgc cgagctggtg cgaacctata cgctcaaccg
     2761 cggattcacc atctcacagt tctgcggcac gcgggtctcg gacaacacgc aggcgtgttc
     2821 ggggacgcac ttccagacca tgcacatctc attcatgcgt ccttcgacga atacgcggat
     2881 cttcgggagg accatcgacg gggtgcagac ggcgctttct gacgcgacga ttcggttgcg
     2941 cgcccccgat gggggtgcct tccgctgcat cacggtctcg caggtcggcc agatcgccgt
     3001 aggcgcatgc ccatgagaac accgaacatt tcccggggct attcgctcgt cgagctcatc
     3061 gtcgcggtcg gcatcttcag catgacgacg ctcatcgtga cgagcgtgta cctcgcgctc
     3121 gtgaacctcg atcgccaggc gcgggcgacc aatcagctcg ccgcgaatct cgacttcgcg
     3181 atggagagca tggtgcgcaa tatccgcacg ggccgcgatt acgaatgtcc gcccccgaac
     3241 tgcaatctca ccttcaccga ctcggaagac gtcctcacgc actaccggga gaacagcgac
     3301 ggtacgatcg ggatctgcac cggggatgat gccctgaatt gcacggatga gaacgcattg
     3361 ccgctgaccg acccacagat caccgtcgag gatctccgct tctatgtgcc ggacgacgaa
     3421 taccaaccgc gcgtcgtcat cgtcgcggag ggcactatgg agacgaacct cggggaggaa
     3481 atcaggttca gcatccagtc ctcggcggc
//
LOCUS       DASPKG010000078         9244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1872982, whole genome shotgun sequence.
ACCESSION   DASPKG010000078 DASPKG010000000
VERSION     DASPKG010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872982"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VEA36_03130"
     CDS             <1..713
                     /locus_tag="VEA36_03130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYF29330.1"
                     /translation="RGLAAYQEDAGERLLRLLLTRDFEKRPGTVFRYTNIPAALLGLV
                     VERVYGATLDKLADSHFFKPLGMSRSTFYPETYPLEEIPPTEVDSWRGEVRGIVHDES
                     AWLAKKDHTIFGHAGLFSTADDLLTFMEMLLNGGEAGGRKFFSEEIITQMETNHIPEL
                     HDSTGLGWELNQPRFMGRHCTPKTFGKTGFTGSLCAIDRSKEIAYVILSNRTYPQRPP
                     DSAAINAFRAAVGEVVFS"
     gene            798..2027
                     /locus_tag="VEA36_03135"
     CDS             798..2027
                     /locus_tag="VEA36_03135"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYF29331.1"
                     /translation="MNRSPLLIIVAAVVLLGGGWLAYDYLKEKPPPFLIKWLTATDKP
                     PPLPAGDQAPLTVPEGFVATIFSRETPGARVMIRDPKGTMLVSLTAEGRVVALPDLNT
                     DGKADETITVLEGLRQPHGLLIRCPDTGNPSADQDACVLYIAETGALKSYAYDADTYT
                     ARYQETLATFPTGPGHFTRTLLMHPDGVRLLASVGSSCNVCIEADERRASVLAVNLDT
                     KEVTTFAKGLRNTVFMAVHPVTGEVWGTDNGRDVIGDDIPPDEVNIIREGRNYGWPLC
                     YGKRVYDTDFGESSADACTATEPSHIDLQAHSAALGLAFIPTQGWPQELEGDLLMAFH
                     GSWNRSEPTGYKVVRVDLDAQGNPQGAPVDFVTGFLAPGASEDKAIGRPVGLLVEPGV
                     VYVSDDRAGAIYRIAIE"
     gene            2108..2839
                     /locus_tag="VEA36_03140"
     CDS             2108..2839
                     /locus_tag="VEA36_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29332.1"
                     /translation="MDAPTDSGSAPVEPRRRSWRWLKITLVVLAGIALLLVLTYREWL
                     PRVIVGYGLIAMPEREFGPVSDIVLSEPPPSGLRYEHQGVSLTVPWTDFHSYRATSTL
                     AVRFGSTTEDVRTIALFYDDLHMKRLIETPKEHAFYGGTLTTNYDVYRALHEVSREDV
                     NLLAPPAEFMPTFILLMLKNVTLVTLPAYRFENMHGIRGYLYQNSATSSMSKFFTPTD
                     RDFTLIIGGATQAERDAILQSIQEL"
     gene            2836..4029
                     /locus_tag="VEA36_03145"
     CDS             2836..4029
                     /locus_tag="VEA36_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HYF29333.1"
                     /translation="MRSPDFREACHTLLKANRRSHHGVQYTVPSPSTYPYQWLWDSCF
                     HAIVLAKLEPEAAIAELRSLLSRQQEDGLVPHLIFWQPKFSHPYHVWWDKSGKSSITQ
                     PPMIAYAAWEIYKETNDDAFLQELYPSLLKFYKYLIEKRDPHDHHLISIINPDESGED
                     NSPRFDVAMRVKSNISFFAHMYKRHQLVVANRTCAFDPLTCMHERFWVKDVPFNVIMI
                     RNLNILAQIAIIMGDAESERYLKQHAELISFTMREYLMAEGVFWSAIGLEHGHLKVAT
                     WAHFIPLFAGLYTKDEADALIQHHFHNPSTFYSPYGIRTVSKQEPSYRPDGFWRGPIW
                     FAPHWFIYKGLKSYGYEKEAAWIKEVSRGHIDKNGFREYFNPETGKAYGAHNFTWGTL
                     VLDMD"
     gene            4032..4664
                     /locus_tag="VEA36_03150"
     CDS             4032..4664
                     /locus_tag="VEA36_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29334.1"
                     /translation="MEGGKMIWEGFEKKNVIPFNPEAFKLALSASGGSIIEEAERQHE
                     TKINTERFELFPPDILRSMELMAKAAEYYLRGPMIQDPFGDDDTIDPWKRREWRASLV
                     TEFGDEARGLLEMNRMRPNLMTTIYAQADRLNRKLQVARLNSPDAARVHKAVQAILAP
                     MEPLEGFGQRYIHAPLNERVEVVRMVSAAARDYLNLVTEQEHDSRLSQAA"
     gene            complement(4661..5389)
                     /locus_tag="VEA36_03155"
     CDS             complement(4661..5389)
                     /locus_tag="VEA36_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYF29335.1"
                     /translation="MRIAVINLQSGIGITKGYREYFTSGWRYLLPHSTKYVRETGQLL
                     KDEGVDIALMTEVEEHGLRSGFRKQTEILREASNLPFSEFFATRSMEPLAAEGNAILC
                     RYPVLETRSHLLTPGAIPRVMGEAMLSIEDQKVHVLVAHLGIRNVYRARQIERIRHII
                     QDRDEPVILGGDFNERNHESFEILRSAGFSHIATLPTYPSWKPVHALSVLFLSKHFSE
                     PRVYIPSAKRFSDHLPLVVEATLA"
     gene            5485..5557
                     /locus_tag="VEA36_03160"
     tRNA            5485..5557
                     /locus_tag="VEA36_03160"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5518..5520,aa:Thr,seq:ggt)
     gene            5598..6185
                     /locus_tag="VEA36_03165"
     CDS             5598..6185
                     /locus_tag="VEA36_03165"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HYF29336.1"
                     /translation="MTTSRIILALFTAGYLIAAAVYFTSLGNTEFLGYIGAVTVLLLI
                     VACTLHYTCFPDWLLWLLSLLMLLHILGGGLMVGGDVLYNYVPLPIENPTGLTFIKVD
                     QIVHTYGSAVAALFVYSFLRRRTDFSWLSMLLITVLAACGIGAINEIIEFTTTLTVED
                     VNVGGYYNTAVDLVVNLVGAIIGAALGLWFWKRTA"
     gene            6207..6279
                     /locus_tag="VEA36_03170"
     tRNA            6207..6279
                     /locus_tag="VEA36_03170"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6240..6242,aa:Thr,seq:cgt)
     gene            complement(6333..7049)
                     /locus_tag="VEA36_03175"
     CDS             complement(6333..7049)
                     /locus_tag="VEA36_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29337.1"
                     /translation="MRITAYQFDHACSRSYRKFEHRYAELWKLFRSTLTFDEIGSILS
                     ITKQAAHQLHDRWFSQYLGPGSERTRFLRQLKRQIERETALEQKLAAARTNPLFVYIS
                     ESAREHGHELEPVIDYEGTGVLASSTPVYRDGERYVRIYHRYAEFRPSQHSTQSYASF
                     QISLSMLTEIYACILFSDIPGFGREIFVVRANLLRNVIKDNGARVWNFYIALGGRKVQ
                     TSKVRVDLRKFKDAWWVLDL"
     gene            7179..8048
                     /locus_tag="VEA36_03180"
     CDS             7179..8048
                     /locus_tag="VEA36_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HYF29338.1"
                     /translation="MNPEERLKEIETLMASPDFWSDKDEAQRVVREYQSLKDGRDGDD
                     LDGGSATLAILAGAGGDDAEDFAAMLRRMYEGYASKQGWSVWELHANENDHGGYRNLL
                     LEISGKGAYGRLKREAGVHRLVRISPFNANAKRQTSFALVEVLPKLAGQQKVELKPDE
                     LDIQFAKSGGAGGQNVNKRETAVRIVHIPTNVSVHVSSERSQQANREKAEALIRAKLY
                     RMEEERREAEAKGRSVATNTANEWGSQMRSYVLHPYKLVKDHESGHESHDPDAVLGGD
                     LDGFIDAAREVGV"
     gene            8065..8748
                     /locus_tag="VEA36_03185"
     CDS             8065..8748
                     /locus_tag="VEA36_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYF29339.1"
                     /translation="MIYFDKVTKIHRDGTAGLDQVTLQIAPKEFVSVVGHSGAGKTTL
                     LRMLLAEDRPTEGSVFFEDIDVHAVPRSALHHYRRKIGMVFQDFRLIPHKTAYENIAF
                     AMEAAGRPQEEIDTDVPHILSLVDLGDKAHHFPHQLSGGEQQRVAIGRAIINQPDVIV
                     ADEPTGNLDPVNTFEIMDILKRINELVGTTVLITTHNKGVVDALGKRVVTMEKGKIIR
                     DDAQGQYVL"
     gene            8755..>9244
                     /locus_tag="VEA36_03190"
     CDS             8755..>9244
                     /locus_tag="VEA36_03190"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HYF29340.1"
                     /translation="MIGTTVKRIISSGAKNFARSGAVSFATVLIMTVTLVIVGFLIFL
                     SALLTHTLALVQDKVDVNAYFTVDAAESDILVVKENLEALPEVASVTYTSREQALAEF
                     RERHAGDQLTLQALEELGENPLGASLAIKAKDPSQYEAIVNQLASDPATVVGGVDIVD
                     RIN"
BASE COUNT         1957 a         2717 c         2695 g         1875 t
ORIGIN      
        1 gccgcggcct cgctgcgtat caggaagatg cgggagaacg gttgctgcgg ttactcctca
       61 ctcgtgattt tgagaagcgt cctggaaccg tattcaggta caccaatatt ccggcagcgc
      121 ttcttgggct ggtggtcgag cgggtgtatg gagccaccct cgacaagctt gccgacagcc
      181 atttcttcaa gccgcttggc atgagtcgaa gcacattcta tccagagacc tacccgctcg
      241 aggaaattcc cccaacagaa gtagatagct ggcggggaga agttcggggc atcgtgcacg
      301 acgaaagcgc gtggcttgct aagaaagacc atacgatctt cgggcatgcc ggtctattct
      361 caactgccga tgacctcctt acgtttatgg agatgctgct taacggaggg gaagcgggag
      421 gccggaagtt cttttcagaa gaaatcatta cccagatgga gaccaaccac attcctgaat
      481 tgcacgactc taccgggtta gggtgggaat tgaatcaacc acgctttatg ggtaggcact
      541 gcacccccaa gacatttggg aagacgggat ttacgggtag tttatgcgct atcgatagaa
      601 gcaaggagat tgcgtatgtg attctttcaa acaggacgta tcctcaacgg ccacctgact
      661 ccgccgccat aaacgccttt cgtgcggccg ttggagaggt cgtcttttcg tagcttcaat
      721 atcttgtgcc ccagcccgac cgcatcgtga ttattcgctc acacgaggtt catggttacg
      781 ggtagacaat tttcctcatg aaccgcagcc ccttactcat cattgtggca gctgtcgtgc
      841 tacttggcgg tggatggctc gcctacgatt atctcaaaga gaagccacca ccctttctca
      901 tcaaatggct gaccgcaacg gataagccgc cgccgcttcc cgcgggagac caggcgccgc
      961 ttacggtacc ggagggattc gtggcgacga ttttttcgcg cgagacgccc ggcgcgcgcg
     1021 tcatgatccg cgatcctaag gggaccatgc tcgtatcgct taccgccgag ggacgggtcg
     1081 ttgcgctccc tgaccttaac accgacggga aggcggacga gacgattacc gtgcttgaag
     1141 gactcaggca gcctcacgga ctgctcatac gctgtcccga cacggggaac ccgagcgcgg
     1201 accaggacgc ctgcgtgctc tatatcgcgg agaccggggc gctcaagtcc tatgcctacg
     1261 acgccgacac gtataccgcg cgctaccagg agaccctcgc gaccttcccg acgggtccgg
     1321 gacacttcac gcgcacgctt ctcatgcacc cggacggcgt gcggctcctc gcatcggtgg
     1381 gctcctcctg caacgtctgc atcgaagccg acgagcggcg tgcatcggtg cttgcggtca
     1441 atctcgacac caaggaggtg accacctttg cgaaggggct ccgcaatacg gtcttcatgg
     1501 ccgtgcaccc ggtaacgggg gaggtgtggg gaaccgataa cggccgcgac gtcatcggcg
     1561 acgacattcc tcccgacgag gtgaacatca tccgggaagg aaggaactac ggctggccgc
     1621 tgtgctacgg caagcgcgtc tacgacaccg acttcggcga gtcgtcggcg gatgcctgta
     1681 ccgccaccga gccctcgcac atcgatctcc aagcgcattc agccgcctta ggacttgcct
     1741 tcattccaac gcagggctgg ccccaggaac ttgagggcga ccttctcatg gccttccatg
     1801 gctcctggaa ccgctctgaa ccgacggggt acaaggtagt ccgtgtggac cttgatgcgc
     1861 agggaaatcc ccagggagct cccgtcgatt tcgttacggg ctttcttgcg ccgggcgcct
     1921 ccgaggacaa agcgatcggg cggccagtcg ggcttcttgt cgagccgggc gtcgtctatg
     1981 tgtcagacga ccgggccgga gccatctacc gcattgccat agagtaacca tttttgggcc
     2041 tgcgtatcgg ggcgtgggta cggcaccagg cagggaaggg taggtaacgg gtgctaccat
     2101 aagcgctatg gatgcaccga cggattcggg atcggcaccg gtcgagcctc ggcgtcgctc
     2161 ctggcggtgg ctcaagataa ccctggttgt tctcgctggc atcgctctcc ttctggtcct
     2221 tacctaccgc gagtggctgc cgcgagtcat tgtggggtat gggctcatag cgatgccgga
     2281 gcgagaattc ggtcccgttt ctgatattgt cctctcagag cctccaccct cggggctgcg
     2341 ctatgagcat cagggggtat cacttaccgt cccatggacc gattttcatt cctatagggc
     2401 gaccagtacg ctcgcggttc gctttggttc gacgactgag gacgtgcgca ccatcgcgct
     2461 cttttatgac gatcttcata tgaagcggct catagagaca ccgaaagaac atgcattcta
     2521 tggagggacg ctcacgacca attatgacgt ctatcgcgcg ctccacgagg tgagccggga
     2581 ggacgtaaat ctgcttgcgc ctccggctga attcatgccc acgttcatcc tcctgatgct
     2641 caagaatgtg acgctggtca cgctgcctgc gtatcggttc gaaaacatgc atggcatccg
     2701 cggctatctg tatcagaatt ctgcaacctc gagcatgagt aagtttttta ccccgactga
     2761 ccgcgatttc acgctgatta tcggaggtgc cacccaggcg gagcgcgacg ctatacttca
     2821 atcgatccaa gagttatgag aagtccggat ttccgcgaag cctgccacac gctcctcaag
     2881 gcgaaccggc gctcgcacca tggcgtccaa tacacggtgc cgtcgccctc cacctatccc
     2941 tatcagtggc tctgggattc ctgcttccat gcgatcgtgc tcgccaagct cgagcctgag
     3001 gctgcgattg cggagttgcg ctctttgctg tcgcgccagc aggaagacgg cctcgtgccg
     3061 cacctgatct tctggcagcc taaattctca catccctacc acgtgtggtg ggataagtcc
     3121 ggaaaaagct cgatcacgca gccgcccatg atcgcgtacg ccgcctggga gatctataag
     3181 gagacgaacg acgatgcctt tctccaggaa ctctatccga gtctcctcaa gttctataag
     3241 tacctcatcg aaaagcgcga tccccacgac caccacctca tcagcatcat caatccggac
     3301 gagtcggggg aggacaactc accgcgcttc gatgtcgcga tgcgggtgaa atcgaacatc
     3361 tccttcttcg cccatatgta caagcgccac cagctcgtag tggctaaccg cacctgcgcc
     3421 ttcgatccct tgacctgtat gcacgagcgc ttctgggtga aggacgtgcc gttcaatgtc
     3481 atcatgatcc gcaatctcaa tatcctggcg cagatcgcga tcatcatggg ggacgcggag
     3541 agcgaacggt acctgaaaca gcacgcagaa ctcatctcct tcacgatgcg cgagtacctc
     3601 atggccgaag gagtgttctg gtccgcgatc gggcttgagc acgggcacct caaggtcgcc
     3661 acctgggctc acttcatccc cttgtttgcc gggctctaca ccaaggacga agcggacgcg
     3721 ctcatccagc accacttcca taatccctcg accttctatt caccctacgg catccggacg
     3781 gtctcaaagc aggagccctc gtatcgcccg gacgggttct ggcgcggtcc catctggttt
     3841 gcgccgcact ggttcatcta taaaggcctc aagtcctacg ggtacgagaa ggaagcggcc
     3901 tggatcaagg aggtgagcag ggggcatatc gataagaacg gcttccggga gtacttcaat
     3961 cccgagaccg gaaaagcgta cggtgcacac aacttcacct ggggcacgct cgtgttagat
     4021 atggattaac tatggaagga ggcaaaatga tctgggaagg gtttgaaaag aagaacgtga
     4081 ttccattcaa tcccgaagcg tttaagctcg cgctttcggc gagcggaggc tccattatcg
     4141 aggaagcgga gaggcagcat gagacgaaga taaatacgga gcggttcgag ctattccctc
     4201 cagatattct gcgctccatg gaactgatgg cgaaggctgc cgaatactat cttcgggggc
     4261 ctatgattca ggatcccttt ggtgacgacg acacgatcga tccatggaag cgtcgcgaat
     4321 ggcgagcatc gctcgtgact gagttcggag atgaagcaag ggggttgctt gagatgaatc
     4381 gaatgcgccc caatctcatg actactatct atgcccaggc ggatcggctc aatcgaaaac
     4441 tacaggtagc gcgcctcaat tctccggatg cagcacgagt acataaagcc gtacaagcga
     4501 tacttgcacc catggaaccg cttgaagggt ttggtcagcg ctatattcac gccccgctta
     4561 atgaacgtgt tgaggtcgta aggatggtga gtgctgcagc ccgcgattac ctaaatcttg
     4621 ttacagaaca ggaacacgat tcgcgtcttt cccaggcagc ctaggccagc gttgcttcaa
     4681 ccaccaatgg gagatgatcc gaaaaccgct ttgcacttgg gatatacacg cgcggctcac
     4741 taaagtgttt cgagagaaac agtaccgata acgcatgcac cggcttccag gaggggtagg
     4801 tgggaagcgt ggcaatgtgc gagaagcccg cggacctgag aatctcaaac gattcatggt
     4861 tacgttcgtt gaagtccccg ccgagaatca ccggctcgtc ccggtcctgg atgatgtgcc
     4921 gtatgcgctc gatctgccgt gcacggtaca cattccgaat gcctagatgt gcgaccagga
     4981 catgcacctt ctgatcctca atcgagagca tcgcttcgcc catgacccgg ggaatggctc
     5041 ctggggtgag cagatgcgag cgggtctcaa ggacggggta gcgacaaagg atcgcattac
     5101 cctcagccgc aagcggctcc atcgaacggg tagcgaaaaa ctcgctgaac ggcagattcg
     5161 atgcctcccg cagtatctcc gtctgcttgc ggaaccccga acgcagccca tgctcctcga
     5221 cctcggtcat gagcgcgata tccactccct catccttaag gagctgcccg gtctctcgca
     5281 cgtatttagt cgaatgagga aggagatacc gccagccgga ggtgaagtac tcccggtatc
     5341 ccttcgttat cccaataccg gattggagat taatgaccgc aatgcgcatt cggatgagta
     5401 taggaagctg agtttgagcg tctggtgaag ttgaaatgca ggggtaaata ccgtactctg
     5461 caacagtcgc cttcgggcga cattgccgat gtagctcagt tggtagagcg cgtccatggt
     5521 aaggacgagg tcgccggttc aatcccggcc atcggctcag gcaatcttca cggtcacctg
     5581 tatatgtaca gatgaccatg accacttcac gcatcatcct cgcgctcttc accgcgggat
     5641 acctcattgc cgctgccgtg tatttcactt ccctcgggaa cacggagttc ctcgggtaca
     5701 tcggggcggt gacggtattg cttctgatcg tcgcctgcac gctccattac acctgcttcc
     5761 cggactggct gctctggctc ctctccctgc tgatgctcct ccatatcctc ggcggaggac
     5821 tcatggtagg cggcgacgtg ctctacaact acgtgccgct tccgatcgag aacccgacgg
     5881 gcctgacctt cattaaggtg gaccagatcg tgcatacgta tggctcagcg gtcgcggcac
     5941 tcttcgtcta ttcgttcctg cggcgtcgga cagacttctc ctggctctcc atgctcctca
     6001 tcaccgttct tgcggcctgc ggcatagggg cgatcaacga gattatcgag ttcaccacga
     6061 cgctcacggt tgaggacgtg aacgtgggcg ggtattacaa caccgcggtg gaccttgtcg
     6121 tgaatctggt gggcgcgatc atcggggcgg cgctcggcct ctggttctgg aaacgtaccg
     6181 cctgatgcgg tattgtaccg aagcaagcca ccgtagctca gttggtagag cgtgtcactc
     6241 gtaacgacaa ggtccccggt tcaatcccgg gcggtggctc ccaaaaacac ggacgcctcc
     6301 ctggccgttt ggccggggag gcatctgaag ttctagaggt cgaggaccca ccaggcgtcc
     6361 ttgaacttgc gcagatccac acgcaccttc gaagtctgca ccttacggcc gccgagcgcg
     6421 atgtagaagt tccatactcg cgccccgttg tccttaatga cgttacgcag aaggttcgca
     6481 cggacgacga agatttcgcg gccgaatcct gggatatcgg aaaataatat gcacgcgtat
     6541 atctcagtga gcatagacag cgagatctgg aacgaagcat aggactgtgt cgaatgctga
     6601 gacggccgga attccgcata tcgatggtag atccgaacat accgctcgcc gtcccgatac
     6661 accggcgtcg agctcgcgag gactcctgta ccctcgtagt cgatcactgg ctcgagttca
     6721 tggccgtgct cgcgcgcgga ctctgaaatg tacacaaaca aaggattcgt gcgcgctgcc
     6781 gcaagcttct gctcgagtgc agtctctcgt tcgatctggc gcttcagctg gcgcaaaaag
     6841 cgggtccgtt cgctccctgg tccaagatac tggctgaacc aacgatcgtg cagctggtgg
     6901 gcagcctgct tcgtgatcga cagtatgctc ccgatttcgt cgaacgtgag tgtacttcgg
     6961 aagagcttcc agagttcggc gtagcggtgc tcaaacttgc ggtagctcct cgagcacgca
     7021 tggtcgaatt ggtaggccgt gattctcaca gccgttgtat ctatattcag ctgcttcctc
     7081 acgataacct cccaagttgt gacagaacgt tcgagtgaaa tataccagag aaactcgatt
     7141 tagcaatttt ccgattacga ctttgctata ctccccgcat gaaccccgag gaacggctca
     7201 aggagatcga gaccctcatg gcctcgcctg acttctggag cgacaaggac gaggcgcagc
     7261 gcgtcgtgcg cgagtatcaa tcgctgaaag acggcaggga cggcgatgat ctggatggcg
     7321 ggagcgcgac cttggccatc ctcgcgggcg ccggcggcga cgatgcggag gacttcgccg
     7381 ccatgctccg gcgcatgtac gagggatacg cgagcaagca ggggtggagc gtatgggagc
     7441 tccacgccaa cgagaacgat cacggggggt accgcaacct cctgcttgag atctcgggga
     7501 agggggcgta cggacgcctg aagcgggaag ccggggtgca ccgactggtc cgcatctcac
     7561 ccttcaacgc aaacgccaag cgccagacct cctttgcgct cgtcgaggtg ctcccgaagc
     7621 tcgccgggca gcagaaggtg gaactcaagc cagacgagct cgacatccag ttcgccaaat
     7681 caggcggcgc aggcggccag aacgtgaaca agcgggagac ggcggtccgc atcgtgcata
     7741 tcccgaccaa tgtatcggtg catgtgtctt cggagcgcag ccagcaggcg aatcgcgaga
     7801 aagctgaagc gctcatcagg gcaaagctct accgtatgga ggaggaacgg cgggaagcgg
     7861 aggcgaaggg ccgctcggtg gccacgaata ccgccaacga atggggcagc cagatgcgct
     7921 cctacgtgct ccatccctat aaattggtga aggaccacga atcggggcat gaatcgcatg
     7981 acccggatgc ggtacttggg ggcgatttgg acggtttcat agacgccgca cgcgaggtcg
     8041 gggtataatg cctttcgttg ttgcatgatc tacttcgaca aggtcactaa aatccatcgt
     8101 gacgggaccg ccggtctcga ccaggtgacc ctccagatcg ctcccaagga attcgtctcc
     8161 gtggtcgggc actcgggcgc aggcaagacg accctgctgc gcatgctcct tgcggaagac
     8221 cggccgaccg aaggctcggt cttcttcgag gacatcgacg tgcatgcggt gccccgcagc
     8281 gcgctccacc actatcgccg gaagatcggg atggtcttcc aagatttccg cctcattccc
     8341 cacaagaccg cgtacgagaa catcgcgttc gcgatggagg cggcgggacg cccccaggag
     8401 gagatcgaca cggacgtgcc gcatatcctg tcgctcgtcg acctcgggga taaggcgcat
     8461 catttcccgc accagctctc gggcggggag cagcagcggg tggcgatcgg gcgcgccatc
     8521 atcaaccagc cggacgtgat cgtcgccgac gagcccaccg gcaacctcga ccccgtgaat
     8581 accttcgaga tcatggacat cctgaagcgg atcaacgagc tggtgggcac cacggtgctc
     8641 atcacgaccc acaacaaggg agtggtggac gcgctcggca agcgcgtggt gacgatggag
     8701 aaggggaaga ttatccgcga tgacgctcag ggccagtacg tcctgtaatt ttttatgatc
     8761 ggaaccaccg taaagagaat catctcgagc ggcgcgaaga acttcgcgcg cagcggcgcc
     8821 gtctcgttcg cgaccgtgct catcatgaca gtgacgctcg tcatcgtcgg cttcctcatc
     8881 ttcctctcgg cgctcctcac ccatacgctc gccttggtgc aggacaaggt agacgtgaac
     8941 gcgtacttca cggtggacgc ggccgagagc gacatcctcg tggtgaagga gaacctcgag
     9001 gcgctgccgg aggtcgcgtc cgtaacctat acgtcccgcg agcaggcgct tgcggaattt
     9061 cgggagcggc atgcgggcga ccagctgacg ctccaggccc tcgaggagct cggggagaac
     9121 ccgctcgggg cctccttggc catcaaggcg aaggatccgt cccagtacga ggcgatcgtg
     9181 aaccagcttg cgagcgaccc ggcgacggtg gtcggcggcg tggacatcgt cgaccgcatc
     9241 aact
//
LOCUS       DASPKG010000079         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1059582, whole genome shotgun sequence.
ACCESSION   DASPKG010000079 DASPKG010000000
VERSION     DASPKG010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059582"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VEA36_03195"
     CDS             <1..623
                     /locus_tag="VEA36_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HYF29341.1"
                     /translation="KLDAAPLSAILKAWQGLGYNRRAKLLHAAAKEVVRTYKGKMPRD
                     AETLRTLPGIGPYTARAVAAFADNADGIFIETNIRTVITHHFFQRKGIVSDGEVLAVL
                     ERVHPKGRAREWYAALMDYGSYLKRSGVRINARAKGYTKQAAFKGSNREARGAILKAL
                     LSGPKTKAKLAPLLGREREAQLLQALAALVEEGLVERAGTRFSVAS"
     gene            complement(551..1774)
                     /locus_tag="VEA36_03200"
     CDS             complement(551..1774)
                     /locus_tag="VEA36_03200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYF29342.1"
                     /translation="MQPQKVLWLGNFFSAAHFFLIVLVVGPYLTTFLGAEAAGLVVSV
                     GAIITLLLFPLMPALVAKKGAKKLAVGLGFLQAVVLTVLAGNPTAAVAIVCVALACAI
                     SPLIAYQLDLLLEATVKEEGETGRIRTAFITAGNIALLAAPLIIGLALNGTDRYDFAF
                     FAAAISLTPFIMLFLVEKLPEGAPPRPSKLRATATCMFRDPDMRAVALGSGTLQVFFH
                     LAPLYVPIYLHTVLGMPWDQLGWMFAIAVLPFILLEYPAGYLADRYLGDRKLLVTGFV
                     IMGLSFASLALVTAATPVYLILLVMLLTRVGAALAEAMVEGHFFRRVTERDASTVSVF
                     RMMRPGGALIAPIMGSLLLVFGGYSMLFVVMGLAIALVGSVSALAMRDVRYDATENLV
                     PALSTSPSSTSAASA"
     gene            complement(1859..>2374)
                     /locus_tag="VEA36_03205"
     CDS             complement(1859..>2374)
                     /locus_tag="VEA36_03205"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HYF29343.1"
                     /translation="PAYGYYSMTASHAKAVEQAGRPRESIVVADNGMVIDIVDGEKLE
                     IHKQMVPSAPLVVDGFSIGDMQEVVIRDRQMLAKDGMFVIIATVNLKTGKLRKSPDII
                     SRGFVYLRESQDMLNQARLLIKKTVEDSTKGMNPVDLDYVKNALSDTMAGYLFARTNK
                     APMVIPVLIGV"
BASE COUNT          481 a          713 c          823 g          357 t
ORIGIN      
        1 agaagcttga cgctgcacca ctgtccgcaa tactcaaagc ctggcagggg ctcggctaca
       61 accggcgggc gaagctgctg catgcggcgg cgaaggaggt ggtgcggacc tacaaaggca
      121 agatgccacg ggacgccgag acgctccgga cgcttccggg catcgggccg tataccgcac
      181 gcgcggtcgc cgcgttcgcg gacaacgccg acgggatttt catagaaacc aacatccgca
      241 ccgtcatcac ccatcacttc ttccagagga aggggatcgt ctcggacggg gaagtgctcg
      301 cggtgctcga gagggtgcat ccgaagggac gagcgcgcga gtggtacgcc gcgctcatgg
      361 actacggctc gtacctgaag cgcagcggag tacgcatcaa cgcacgcgcg aagggataca
      421 cgaagcaggc ggccttcaag ggctcgaatc gggaagcccg cggcgcgatc ttgaaggcgc
      481 tccttagcgg cccgaagacc aaggcaaagc tcgcgccact actcggacgc gagcgagaag
      541 cgcagctcct tcaggcgctc gccgcgctcg tcgaggaggg gctcgtggag agagccggga
      601 cgaggttctc ggtggcgtcg tagcgcacgt cgcgcatcgc aagcgcgctc acggagccga
      661 cgagtgcgat cgcgaggccc atcaccacga agagcatcga gtagcccccg aagacaagca
      721 ggaggctgcc catgatgggt gcgatgagag cgccgcctgg ccgcatcatg cggaagacgg
      781 agaccgtgct ggcgtcgcgc tccgtaaccc ggcggaagaa gtgcccctcc accatggcct
      841 ccgcaagcgc tgcgcccacg cgggtgagca gcatcacgag gaggatgagg tagacggggg
      901 ttgcggcggt cacgagcgct aaggacgcga acgagagccc catgatcacg aagcccgtca
      961 cgagaagctt gcggtccccg aggtagcggt ccgcgaggta cccggccggg tactccaaga
     1021 ggatgaaggg aaggaccgcg atcgcgaaca tccacccgag ctggtcccag ggcatgccga
     1081 gcacggtatg gaggtagata gggacataga gcggagcgag atggaagaag acctggagcg
     1141 tgccgctgcc gagcgcgacc gcccgcatat ccggatcgcg gaacatgcag gtcgcggtgg
     1201 cccgcagctt cgagggcctg ggcggcgctc cctccgggag cttctcaacg aggaagagca
     1261 tgatgaacgg cgtgagcgag atcgcggcgg cgaagaaggc gaaatcgtag cggtcggtgc
     1321 cgttgagcgc gagcccgatg atcaatggcg ccgcaagcag cgcgatgttg cccgccgtga
     1381 tgaaggcggt gcggatgcgc ccggtctcac cctcttcctt gacggtggct tcgaggagga
     1441 ggtcgagctg gtaggcgatc agaggcgaga tggcgcaggc gagcgccacg cagacgatcg
     1501 cgaccgcggc cgttgggttc ccggcgagga cggtgaggac cacggcctgc aggaatccga
     1561 gcccgaccgc gagcttcttg gcccctttct tggcgaccaa ggccggcatg agggggaaga
     1621 gcagcagggt aatgatcgcg cccaccgaca ccacgagccc cgcggcctcg gcgccgagga
     1681 aggtggtcag gtagggacct accaccagca cgatgaggaa gaaatgcgcg gcggagaaga
     1741 agttgccgag ccagagtact ttctggggtt gcatgtactg aaaattgtat cattcgtact
     1801 gaacaatgaa tgaagccgcc gcgcgaaatg cgcggcggct tcatcgcgat cagtcgtgct
     1861 acaccccgat caataccggg atcaccatcg gcgccttgtt cgtgcgcgcg aagagatagc
     1921 ccgccatcgt atcggagagg gcgttcttca cgtagtcgag gtcgacgggg ttcatgccct
     1981 tggtcgaatc ctcgaccgtc ttcttgatga ggagccgggc ctggttcaac atatcctggc
     2041 tctcgcgcag gtacacgaag ccgcgggaga tgatgtccgg ggacttgcgg agcttgcccg
     2101 tcttcaggtt caccgtcgcg atgatgacga acatgccgtc cttcgcgagc atctggcggt
     2161 cgcggatcac gacctcctgc atgtcgccga tcgagaagcc gtcgaccaca agcggagccg
     2221 acggcaccat ctgcttgtgg atctcgagct tctcgccgtc cacgatatcg atcaccatgc
     2281 cgttgtcggc gacgacgatc gactcgcggg gccgccccgc ctgctcgacc gccttggcgt
     2341 ggcttgcggt catggaatag tagccgtagg cggg
//
LOCUS       DASPKG010000080         5207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1060065, whole genome shotgun sequence.
ACCESSION   DASPKG010000080 DASPKG010000000
VERSION     DASPKG010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060065"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VEA36_03210"
     CDS             <1..690
                     /locus_tag="VEA36_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29344.1"
                     /translation="KKSDTDFPGTAAAPSSLTERFSQKFLAQYISTRGTAPPTEEEML
                     NFIKDAVADLQASRDASPEFSGRDVTVSGSGRDALVSYAAAVEAAMIGNAYAADKPDL
                     DYFALAIQNNDADSLAKLEKVSEGYRRVARALIKVRAPEEAAAAHLAFVNALFNMGLA
                     TGDLAAVEGDPIRALLGIGGYQQYEGDLARAYQALNAVFVAEGVTVTGQGSAFVEAAA
                     GANRILTQPAP"
     gene            694..2073
                     /locus_tag="VEA36_03215"
     CDS             694..2073
                     /locus_tag="VEA36_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29345.1"
                     /translation="MNNLSRKTLALTLALLIMLPTMAGAFPRSAHAQLIVHSPLEYEA
                     LMQLRLKEAGLLGGAFSLDGIAWVLAKTAISTMTKSLVNWINSGFQGSPAFATDLRQN
                     LLRLGDAYAARFFDEITNEVIESPYRDEIATALRTHYYLSTGGAFYIRNPYTLNQYST
                     NPVDFLNGNFSQGGLSAWFSTVMNAQNNPYTALQLAQQELDSRLASAGGRRITELNWG
                     QGFMSWRGDCARTAAGDSAAFANSYAGGLDTDLNELVAASNAQLADEEGCLEYGIKTP
                     GTVIESAFNNAVPKGMEGLITADEIDEVVAALFQQLITQVVGKTGLLGISAPSSGGGR
                     SFIDQATLPDLQGRPPTIQIDPAVEAKLAAFESGWRLIGEAATAASKNCPQSAAAQNA
                     LKSAVEAKTRVEGVRKALKAGATLEQVGTLMPSDEEVAAAQTSSAEGDGSLRQQLTVI
                     AGLRSCPSS"
     gene            2077..4350
                     /locus_tag="VEA36_03220"
     CDS             2077..4350
                     /locus_tag="VEA36_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29346.1"
                     /translation="MTLLSAPEVYRIAKIAAVIVVVLFSILIAAPSVAQAQSTCPEGT
                     STALGTCRCEYSTYGSRDGKLTTSCRATTSTGEATGDTTTTVTSGLGTGGVTGAQVTD
                     TLNSAGVGTRSVTQPKPEPKSCSITGGTFDIGECVAKALGLFMGWIVGGLALALLTLA
                     SFILWIVGGLFNWVVIKTIFEFGSTFGTSEGMLIAWGILRDIGNILLLFAFIFMGLAT
                     ILNTHALDEYSARKALPRLIIFAVLLNFSLFAAQIVIDVSNSFAAVFTAQAGLSGCTT
                     ANDTVDVRTGLTPREACANNGIAGQVIQMAGISSIFSVQGAKDFALNSDRAAPVYIGL
                     IIFVTITAVVLLAGAIMLVIRAVMLCLLMVLSPIGFAGMAVPPLQEFANRWWKLLLSN
                     AFFAPIYLLMVLISLKIVEGLAGTNSNRSFAVALVEGSTNAPQIFVIFAVVIGFMVMA
                     LVVAKNMGAMGADFATRTAGGAVLGTYGFVARRTAGRYAGKAAEAIRSSPWGQKDRRI
                     FGIPGTSARNISGLLEKTSKSNLSVRGVATTLGKGAHLDFGKPSKAVAHGIHGIEEQA
                     EKDRVEYEKKLKATGEQKANAKEVEKERDAVKQELEDRRAKEFAELEAARLKVAQARL
                     GTDKAALEQARKEFNEALDRYHKRNNDKEGLDLKDRLKKLEARLKYWNDWADQQKTDT
                     YLNVGLENNWYPWFSVGTHADHEAKKTIKKNRGKSKIDKALDDFKKSVKDDKDGGEAD
                     ADADAPKDAGAKPDDTH"
     gene            4356..>5207
                     /locus_tag="VEA36_03225"
     CDS             4356..>5207
                     /locus_tag="VEA36_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29347.1"
                     /translation="MEDKDFFESVSRLLRQAPAPVRDFVLNELPQATERLMQRHQLHI
                     DQGGVLESELLLLLLGQSTPEEFVMSLKEGGVPEEKLKALVGDVNTEIFMRIRKQEQT
                     QKPPEPRPTPPVPPSAPAARTPVPIYSPLPSAPIQILPPPHVMQALEKPQAPKQVAPP
                     ANLPGQVPPTPPPRPAPPPPPHIEMPVPPPPPPREVPHTDAQSRVIHTMSRDMQALKS
                     GVDPLRVAHPVPPGWAEAPNATPLAPPRPIPPPSAPPPQPLPPPMPAHEAPVPPPIIE
                     KPHPQQAL"
BASE COUNT         1015 a         1659 c         1641 g          892 t
ORIGIN      
        1 aagaagtcgg acacggattt cccgggtacg gccgccgcgc cttcctcact caccgagcgc
       61 ttctcgcaga aattccttgc gcaatacatc tcgacgcgcg ggaccgctcc gccgacggaa
      121 gaagagatgt tgaatttcat caaggatgcg gttgccgacc tccaggcctc ccgcgatgcg
      181 tcgcctgaat tctccggacg ggacgtaacg gttagcggat caggacgcga cgcgctcgtt
      241 tcgtacgcag cggcggtcga ggcggcaatg atcggcaatg cctacgccgc cgataagccc
      301 gacctcgact acttcgcgct cgccatccag aacaatgatg cggacagcct cgcgaagctc
      361 gagaaggtct ccgagggata taggcgtgtc gcccgcgcgc tcatcaaggt gcgggcgccg
      421 gaagaggcgg cagcggccca cctcgccttc gtgaacgcgc tcttcaatat gggcctggcg
      481 accggagacc tcgccgcggt cgagggcgac ccgatccgtg ccctgctcgg gatcggcgga
      541 taccagcagt acgagggtga tctcgcgcgc gcgtatcagg cgctcaacgc ggtgttcgtc
      601 gccgagggcg tcacggtaac cgggcagggg agcgcgtttg ttgaagcggc agcgggcgcc
      661 aatcgcatcc tcacgcagcc cgcgccatag cgtatgaaca acctctcccg caaaacgctc
      721 gctctcaccc tcgccctcct catcatgctg ccgaccatgg ccggcgcctt cccgcgcagc
      781 gcgcacgcgc agctcatcgt gcattcgccg cttgagtacg aggccctcat gcagctgcgc
      841 ctcaaggagg cggggctcct gggcggcgcg ttcagcctcg acgggattgc ctgggtgctc
      901 gcgaagaccg ccatctccac gatgaccaag agcctcgtga actggatcaa ctcgggattc
      961 cagggttcgc cggcgtttgc gaccgacctc cggcagaacc tgctccggct cggcgatgcg
     1021 tacgccgcgc ggttcttcga tgagatcacc aatgaggtca tcgagtcgcc ctaccgcgac
     1081 gagatcgcga ccgcgctgcg cacccactac tacctctcga ccggcggcgc cttctacatc
     1141 cgcaatccgt ataccctcaa tcaatacagc accaacccgg tcgacttcct gaacggcaac
     1201 ttctcccagg gcggcctctc cgcatggttc tcgacggtga tgaacgcgca gaacaatcca
     1261 tacaccgcac tacagctcgc gcagcaggag ctcgatagcc ggcttgcctc tgcaggtggc
     1321 cgccgcatca ccgaactcaa ctgggggcag ggattcatgt cctggcgcgg cgactgtgcg
     1381 aggaccgcgg caggggattc cgcggcattc gcgaactcgt acgcgggcgg ccttgatacc
     1441 gatcttaacg agctcgtcgc ggcgagcaat gcgcagcttg cggacgagga agggtgcctt
     1501 gagtacggca tcaagactcc gggaacggtc atcgaatcgg cattcaataa cgcggttccg
     1561 aagggtatgg agggtctcat cactgcggac gagatcgacg aggtcgtcgc ggcgctcttc
     1621 cagcagctca tcacgcaggt ggtgggtaag accggcctcc tcgggatctc cgcgccttca
     1681 agcggcggcg gccgctcatt catagatcag gcgaccctgc ctgatctgca ggggaggccg
     1741 cccacgatcc agatcgaccc ggccgtcgag gcgaagcttg cggcattcga gtccggctgg
     1801 cggctcatcg gcgaggcggc gaccgcggcc tcgaagaatt gcccgcagtc agcggcggca
     1861 cagaacgcgc tcaagagcgc tgttgaggcg aaaacgcgcg ttgagggcgt tcgtaaggcg
     1921 cttaaggcgg gtgcgactct cgagcaggtg ggcacgctca tgccaagcga cgaggaggtt
     1981 gcggctgcgc aaacgtcttc tgcggaaggc gacgggtcgt tgcggcagca gttaacggtg
     2041 atcgcggggt tgcggagctg cccatcgagt tagcctatga cgctgctctc cgctccagag
     2101 gtctaccgga ttgcgaagat cgccgcggtg atcgtcgtcg ttcttttttc catactgatc
     2161 gcggcgccct cagtcgcgca ggcgcagtca acctgccctg aagggacgag tacggcactt
     2221 ggcacctgcc gctgcgagta ctcgacgtac ggaagcagag atgggaagtt aacgacctcg
     2281 tgtcgggcaa ccacctcgac cggtgaggcg actggagata ccaccaccac ggtgacttct
     2341 ggcctcggga cgggcggggt cacgggagcg caggttacgg acaccctgaa cagcgcgggg
     2401 gtagggacac gcagcgtcac acagccgaag ccggagccaa agagctgcag catcaccggc
     2461 ggcaccttcg atatcgggga atgtgtcgcc aaggcactcg gattgttcat gggctggatc
     2521 gtgggcgggc tcgcgcttgc tctgcttacg ctcgcgagct tcatcctctg gatcgtgggc
     2581 gggctcttca actgggtggt gatcaagacc atcttcgagt tcggcagcac attcggcacc
     2641 tccgaaggca tgctcatcgc ctggggcatc ctgcgagaca tcgggaacat cctgctcttg
     2701 ttcgcattca tcttcatggg gctcgcgacc attttaaata cgcatgcgct ggatgaatac
     2761 agcgcgcgca aggcgctccc gcgcctcatc atcttcgcgg tcctcctcaa tttcagcctc
     2821 tttgcggccc agatcgtcat cgacgtctcg aacagcttcg cggcggtctt taccgcgcag
     2881 gcggggctct cgggctgtac caccgcaaac gacaccgtcg atgtgcgcac cgggctcacc
     2941 ccgcgggagg cctgcgcgaa caacggcatc gccggacagg tcatccagat ggccggcatc
     3001 tcgagcatct tctcggtcca gggcgcgaag gatttcgccc tcaattccga tcgcgcagcg
     3061 cccgtataca tcgggctcat catcttcgtt acgataaccg cggtcgtgct cctcgccggg
     3121 gccatcatgc tcgtgatacg cgccgtcatg ctctgcctac tcatggtgct ttcgccgatc
     3181 gggttcgcgg gcatggcggt gccgccgctc caggaattcg cgaaccggtg gtggaagctg
     3241 ctcctgtcga acgcgttctt tgcgccgatt tacctgctca tggtgctcat cagcctcaag
     3301 atcgtcgagg ggcttgcggg cacgaattcc aatcgttcct tcgcggtcgc tctcgtcgag
     3361 gggagcacga acgcgccgca gatcttcgtc atcttcgcgg tggtgatcgg gttcatggtc
     3421 atggcgctcg tggtcgccaa gaacatggga gcgatgggcg cggatttcgc gacgaggacc
     3481 gcgggcgggg cggtgctggg gacgtatggg ttcgtggcgc gaaggactgc tgggcgctac
     3541 gccggcaagg ccgccgaagc cattcgttcc tcgccgtggg gccaaaaaga ccgcaggatc
     3601 ttcggcatcc ccggcacctc tgcccggaat atttccggcc tgctggagaa gacttcgaaa
     3661 tcgaacttga gcgtgcgcgg cgtcgccacc acgctcggga agggcgcgca tctggacttc
     3721 ggcaagccaa gtaaagcggt tgcgcacggc atccatggca tcgaggagca ggcggagaag
     3781 gaccgcgtcg aatacgaaaa gaagctcaag gcgacgggtg agcagaaagc gaacgcgaag
     3841 gaggtcgaga aggagcgcga cgcggtgaag caggagctcg aagaccgccg ggcgaaggaa
     3901 ttcgcggagc ttgaagcggc gcgactgaag gtcgcccagg cacgactggg caccgataag
     3961 gccgcgctcg aacaggcccg caaggaattc aacgaggcgc ttgataggta tcacaagagg
     4021 aacaatgata aagagggcct ggaccttaag gatcgattga agaagctcga ggcacgcctt
     4081 aaatactgga atgattgggc ggaccagcag aagaccgata cgtatctcaa cgtcggcctg
     4141 gagaacaatt ggtacccgtg gttctccgta ggcacgcacg ccgatcatga ggccaagaag
     4201 acgattaaga agaatcgcgg gaagagcaag atcgataagg cgctcgacga tttcaagaag
     4261 agcgtcaagg atgataagga cggcggcgaa gcggacgcag acgcagacgc tccgaaggat
     4321 gcaggcgcaa agccggatga tacgcactaa ggactatgga ggataaagat tttttcgaga
     4381 gtgtatcgcg gctcctgcgt caggcgcctg cgccggttcg cgatttcgtg ctgaatgagc
     4441 ttccgcaggc gaccgagcgg ctcatgcagc ggcatcagct ccatatcgac cagggcggcg
     4501 tgctcgagag cgagctcctg ctgctgctgc tcggacaatc gacacccgag gaattcgtca
     4561 tgtccctcaa agagggggga gtgcccgagg agaaactcaa ggcactcgtg ggcgatgtga
     4621 atacggagat atttatgcgt atccgcaagc aggagcagac acagaagccg cccgagcctc
     4681 gaccgacacc tcccgtgccc cctagcgctc cggctgcccg aacgcccgta cctatatata
     4741 gtccgctccc gtccgcgccg atccagatcc tgccgccccc gcacgtcatg caggcattgg
     4801 agaagccgca agcgcctaag caggtggctc cgcccgctaa tctgccgggc caagtgcccc
     4861 caacgcctcc gccgcgcccg gccccgccac ctccgccgca tatcgagatg ccggtaccac
     4921 cgccgccacc tccgcgagag gttccgcaca cagacgcgca atcccgcgtc atccacacca
     4981 tgtcccggga tatgcaggcg cttaagagcg gggtggatcc gctccgggtc gcgcaccccg
     5041 ttcctcccgg atgggcagaa gcgccgaatg caacgccgct tgcgcctcct cgtccgattc
     5101 cgccgccctc agcgcctccg ccgcagccgc ttccacctcc catgcccgcg catgaggcac
     5161 cggtgccgcc cccaatcatc gagaagccgc atccgcagca ggcgctc
//
LOCUS       DASPKG010000081         6820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_654164, whole genome shotgun sequence.
ACCESSION   DASPKG010000081 DASPKG010000000
VERSION     DASPKG010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654164"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VEA36_03230"
     CDS             <1..569
                     /locus_tag="VEA36_03230"
                     /inference="COORDINATES: protein motif:HMM:NF023166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HYF29348.1"
                     /translation="NGRAIVEGWLTQTVAQFKQDGRFDTLTPDEARFLGYTTDRKQSL
                     TITYGVNVMLGVPSSIDSYVYTITMDTFGAHPNTFFKTFNFDRETGAELALADLFLPG
                     TPYLQELSTIARRELPKEMGEFANYDSIVAGTEPTEQNFANWTTEGSTLTIIFPPYQV
                     AAYAAGPQYLDIPLGWLDDILKPEYRAP"
     gene            complement(544..1398)
                     /locus_tag="VEA36_03235"
     CDS             complement(544..1398)
                     /locus_tag="VEA36_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HYF29349.1"
                     /translation="MTDQVAKNLETIGVVNVMYPNALRTAVEQAVASWKDFCALPEKV
                     RAKFPYNPGEGMGVGYEIKKTPGATLDLKEDFHLTLASREWLLETAATCDEPTILKFV
                     TDAESLISIMRPTVEGFAEVIEQNFDLADFAKDIAGNSDWWFIRFLHYFGNREEGEEI
                     ATAHVDKSAFTLHLYESHPGLQHLTYEGNWEDMPVSEGETAIIPGMRLQYRSDNRLRA
                     TCHRVVATKTTAQEGRYSAVCFIHPKNTPVYDKARAGRLQEFAPGFNYTMSLEEFRNL
                     FTVPDIRA"
     gene            complement(1454..2299)
                     /gene="rplB"
                     /locus_tag="VEA36_03240"
     CDS             complement(1454..2299)
                     /gene="rplB"
                     /locus_tag="VEA36_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HYF29350.1"
                     /translation="MKSFKPTTKSRRQMTVVTYKGVLSGDKPMKSLLSTKKNRAGRNN
                     AGRITTRHQGGGHKRRLRDVDFSYNKKDIPARIETVEYDPYRTGFIARALFADGERRY
                     VLAPKDTKVGDTFIVSETAPIKQGNRMPLGKIPSGTFVYNIELQPGAGARIARSAGNY
                     AEIVAHDAGYAQLKMPSTEIRKISDLAWASIGAVGNEEQRLVNYGKAGRSRWKGIRPT
                     VRGTAMNPVDHPHGGGEGRQGRGLRRAKSMWGKPTGKGQKTRTPKKYSNKFIVARRKV
                     GKKRG"
     gene            complement(2310..2675)
                     /gene="rplW"
                     /locus_tag="VEA36_03245"
     CDS             complement(2310..2675)
                     /gene="rplW"
                     /locus_tag="VEA36_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HYF29351.1"
                     /translation="MALFSSDTKKQKAAKAPTVRRGALHPEDARIANVLKNPWFSEKA
                     LIGTENGVYVFEIPPAVTKHDVMNAIAKTYKVTPVKVNIVNLPAKKKAMRTKRGVGTR
                     ARRHKAYVYLKKGETIAFT"
     gene            complement(2683..3420)
                     /gene="rplD"
                     /locus_tag="VEA36_03250"
     CDS             complement(2683..3420)
                     /gene="rplD"
                     /locus_tag="VEA36_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HYF29352.1"
                     /translation="MAPVKKTVAKKAPAKKTVAKAPAASEAPVYTMAGESAGTVSLPA
                     ELFAQPWKADLVHQVVVGMQANARPTVANTKFRGEVSGGGKKPWKQKGTGRARHGSNR
                     SPIWVGGGTTHGPRAERVYAVKINRKMRSAALASVLSRKLRDGEVIFVDSLTFAAPKT
                     KDAKAALVAVAAAAGKPELVTKWKNAAVVAFAKKDAAAEKSFRNMGNLITEEVRNLNP
                     VDLVTKKFLVIENPKEALPVLASRLTK"
     gene            complement(3431..4042)
                     /gene="rplC"
                     /locus_tag="VEA36_03255"
     CDS             complement(3431..4042)
                     /gene="rplC"
                     /locus_tag="VEA36_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HYF29353.1"
                     /translation="MKFLIAKKGRMTQIFDTDGRVFAGTVLKTTPMTVTQVKTPDTDG
                     YAAVQVGFGTQKESRRGKAVLGHTKGKAYETLKEFRTEAGELTVGSEVSADTFSEGDV
                     VEVSGTVKGRGFAGVVKRHGFHGGRRSHGQKHSEREPGSIGGGGRAGGRVIKGMRMAG
                     RMGGNRITVKNLKVLKVDAASGEIIISGAVPGAPGSLVEIVAK"
     gene            complement(4411..4887)
                     /locus_tag="VEA36_03260"
     CDS             complement(4411..4887)
                     /locus_tag="VEA36_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HYF29354.1"
                     /translation="MGKVTGLFIAAKPGDPMRTVEAVRAFFNLGLEGDRYHYGRKHGD
                     EVCHGVTLFSEEAHTKANAALIHRGIGPYGPHEIRRNIMIAGIEDMTVLVGKDFSVGS
                     VRMRGIEPCEPCMRPARLLERGDGALFMINFAGRAGIRAQVLTDGEIRLEDEVRGA"
     gene            complement(4979..5485)
                     /locus_tag="VEA36_03265"
     CDS             complement(4979..5485)
                     /locus_tag="VEA36_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYF29355.1"
                     /translation="MYITIDRLAEEDLLSRAQLLNYLTEAWKEPREEIAANYIRPSFT
                     ESLPDVFIAFTDRGDVAGHVMLVIEEKWYLGIDGEPWICGLYVPEEYRGQGVGRRLME
                     TAENACRRHGFNHLYLDTAGARDYYLRLGWEEYGEAWWQAQGKTVTVMRKQVMVDRIR
                     RRQFRRSA"
     gene            complement(5573..5938)
                     /gene="rpsJ"
                     /locus_tag="VEA36_03270"
     CDS             complement(5573..5938)
                     /gene="rpsJ"
                     /locus_tag="VEA36_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HYF29356.1"
                     /translation="MATATKKVAKKKAPEAGPAAESKLRIRVRAYEHKILDLSVKQIM
                     DTANRFDAKIVGPIPLPTEVKRWTVNRSTFVHKDAREQFEMRIHKRLIDIMSPNPKVI
                     EALTNLNLPSGVTIDVKMV"
     gene            complement(5970..>6820)
                     /locus_tag="VEA36_03275"
     CDS             complement(5970..>6820)
                     /locus_tag="VEA36_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011233600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HYF29357.1"
                     /translation="TREHILLAKQVGVPRLIVFLNKVDMVDDKDMVDLVEEEIRELLT
                     KQGFDGANTPVIKGSGLKALEATSADDEWALKVKELVDTMDTYFPDPVRETDKPFLMA
                     IEDIFSIEGRGTVVTGRIDRGIAKVGEEVEIVGLKDTVKTTITGIEMFNKQLQEGMAG
                     DNAGILLRGTKKEDVHRGQVLVKPGSITPHTEFEAEVYILSKDEGGRHTPFFSGYKPQ
                     FYVRTTDVTGEVTLPDGKEMVMPGDTVTFKVKLTAPIAMEEQMRFAIREGGKTVGAGV
                     VTKITA"
BASE COUNT         1161 a         2150 c         2059 g         1450 t
ORIGIN      
        1 aaaacgggcg cgcaattgta gaagggtggc ttacgcaaac ggtggcgcaa ttcaagcagg
       61 acggcagatt cgacacgctt actccagacg aggcacgctt tctcggatac accaccgatc
      121 gaaagcagag cctcaccatc acctatgggg tgaacgtgat gctcggggtc ccctcctcca
      181 tcgattccta tgtctacacc atcaccatgg atacgttcgg ggcgcacccc aacaccttct
      241 tcaagacctt caacttcgac cgcgagaccg gcgccgagct tgccctcgcc gatctcttcc
      301 tgcccggcac tccctacctg caggaattga gtacgatcgc gcggcgcgag ctcccgaagg
      361 aaatgggtga attcgccaac tatgactcca tcgtcgccgg gaccgagccg acggagcaga
      421 acttcgccaa ctggacgacc gagggcagca cgctcaccat catcttcccg ccgtaccagg
      481 tcgctgcata cgcggcgggt ccgcagtatc tggatatccc gctcggatgg ctcgacgata
      541 tcctcaagcc cgaatatcgg gcaccgtaaa tagatttctg aactcctcga gcgacatcgt
      601 gtagttaaag ccgggagcaa actcctgtag acgccccgct cgtgccttgt catagaccgg
      661 cgtgttcttc ggatgaatga aacagaccgc agagtatcgc ccctcctgtg ctgtggtctt
      721 tgtcgctacg acacggtggc aggtcgcccg caaccggttg tccgagcggt actgaaggcg
      781 catgccggga ataatggctg tttcgccttc cgataccggc atatcctccc aattcccctc
      841 gtacgtaaga tgctgcaggc ctggatgact ctcgtacaga tggagggtga atgcgctttt
      901 atcgacatgc gccgtagcga tttcttcccc ttcttcccgg ttgccgaagt aatgcaggaa
      961 gcggataaac caccagtcgg agttcccagc gatatccttc gcgaagtccg caagatcgaa
     1021 attttgttca atcacttcgg caaatccctc cacggttggc cgcatgatac tgatgagcga
     1081 ttcggcatcc gtcacgaatt tcaggatagt cggttcatcg cacgtagccg cggtctcaag
     1141 cagccactcc ctcgaggcga gcgtaagatg gaaatcttct tttagatcaa gggtcgcgcc
     1201 tggtgtcttc ttgatttcat acccgacccc catgccttcg cccggattat acggaaactt
     1261 ggcgcgcacc ttttcaggga gcgcgcaaaa atctttccac gacgcaaccg cctgctcgac
     1321 cgcggttcgc agtgcgttcg gatacatgac attcactacc ccgattgttt ccagattctt
     1381 cgcgacctgg tcagtcatat gattcttagt atagctctcc taatcaaaaa gctcccggag
     1441 gggagctttt tgattatcct cgcttcttgc cgaccttccg gcgagccacg atgaacttgt
     1501 ttgaatactt cttcggcgta cgggtcttct ggcccttgcc ggtgggcttg ccccacatgc
     1561 tcttggcacg ccgcagaccg cggccctggc gaccctcacc gccgccgtgc gggtgatcca
     1621 ctgggttcat ggcggtaccg cgcaccgtcg ggcggatgcc cttccagcgc gaacggccgg
     1681 ccttgccgta gttcacgagg cgctgctcct cgttcccgac ggcgccgatg gacgcccagg
     1741 caaggtcgga gatcttgcgg atctcggtcg acggcatctt gagctgcgcg tacccggcgt
     1801 catgcgcaac gatctcggcg tagttgccgg cggagcgggc gatgcgggcg ccggcgcccg
     1861 gctgcagctc gatgttgtac acgaaggtgc ccgacgggat cttcccgagc ggcatgcggt
     1921 tgccctgctt gatcggtgcc gtctccgaga cgatgaaggt atcgcccacc ttggtgtcct
     1981 tgggtgccag cacgtagcgg cgctcgccgt cggcgaacag ggcgcgggcg atgaaccccg
     2041 tgcggtacgg gtcgtactcg acggtctcga tacgcgccgg gatgtccttc ttgttgtacg
     2101 agaagtcgac atcgcgcagg cggcgcttat ggcccccgcc ctggtggcgg gtcgtgatgc
     2161 ggcccgcgtt gttgcggccg gcgcggttct tcttcgttga aagaaggctc ttcatcggct
     2221 tgtcgcccga caggacaccc ttgtaggtga cgacggtcat ctggcgacgg ctcttggtgg
     2281 ttggcttgaa ggatttcata ggtcagggct taggtgaacg cgatggtctc gcccttcttg
     2341 aggtagacat aggccttgtg gcggcgggcg cgggtaccca cgccgcgctt ggtgcgcatc
     2401 gccttcttct tcgcggggag gttcacgatg ttcaccttca ccggggttac cttgtacgtc
     2461 ttcgcgatcg cgttcatgac gtcgtgcttc gtgaccgcag gcggtatctc gaacacgtag
     2521 acgccgtttt cggtgccgat cagggccttc tcggagaacc agggattctt gagcacgttg
     2581 gcgatgcgtg cgtcctccgg gtgcagggcg ccgcggcgga ccgtgggcgc cttcgcggcc
     2641 ttctgcttct tggtgtcgga tgaaaagagt gccatacgcg tattacttag tgaggcggga
     2701 tgcgagcacg gggagcgcct ccttcgggtt ctcgatcacc aggaacttct tagtgacgag
     2761 atcgaccggg ttgaggttcc gcacctcctc ggtgatgagg ttgcccatgt tccggaagga
     2821 cttctcggct gccgcatcct tcttcgcgaa cgcgacgacc gcggcgttct tccacttggt
     2881 gacgagctcg ggcttgccgg ctgcggcggc gaccgcaacg agcgccgcct tcgcgtcctt
     2941 ggtcttcggc gctgcgaagg tgagggagtc cacgaagatc acctcgccat cgcggagctt
     3001 gcgggacagg acggaggcaa gtgccgccga gcgcatctta cggttgatct tcacggcgta
     3061 gacacgctcg gcgcgggggc cgtgggtcgt gccgccgccc acccagatcg gggagcggtt
     3121 ggagccgtgg cgggccctgc cggtgccctt ctgcttccag ggcttcttgc cgccgccaga
     3181 gacctcgccg cggaacttcg tgttcgcgac cgtcgggcgt gcgttcgcct gcatgccgac
     3241 taccacctgg tgcacgaggt ccgctttcca aggctgggcg aagagttccg ccggaagcga
     3301 aacggtgcct gccgactcgc ccgccatggt atagacgggc gcctcgctag ccgctggtgc
     3361 ctttgcaaca gtcttcttag caggagcctt cttcgcgacg gttttcttta ctggtgccat
     3421 ggtattgaaa ttacttcgcg acgatctcga ccagcgagcc cggggcaccc ggcaccgcgc
     3481 ccgagatgat gatctcgccg ctagctgcat cgaccttcag caccttgagg ttcttcaccg
     3541 tgatgcggtt gccgcccatg cggccggcca tgcgcatgcc cttgatgacc cggccaccag
     3601 cgcggccacc gccaccgatc gagcccggct cgcgctcgga gtgcttctgg ccatgcgagc
     3661 ggcgaccgcc atggaagccg tggcgcttca ccacgcccgc gaacccgcgt cccttcacgg
     3721 tgcccgagac ctcgaccaca tccccttccg agaaggtgtc cgcggatacc tccgaaccga
     3781 ccgtaagctc ccctgcctcg gtgcggaact ccttcagggt ctcgtatgcc ttgcccttgg
     3841 tatggcccaa gaccgccttg ccgcggcgag actccttctg cgtgccaaag cccacctgaa
     3901 cggctgcgta gccgtctgta tcaggcgtct tcacctgggt cacggtcatc ggggtggtct
     3961 tcaggacggt accggcaaac acccgaccgt cggtatcgaa gatctgggtc atgcggccct
     4021 tcttggcgat gaggaatttc ataaaaagtt gaatccgcgc ctgcaacagc gcgggagggg
     4081 gtcccttccg taccattggt ccggccgtat gccgtacgta gcgtgtaggc actctacacg
     4141 agggcttcat ggattgcaaa tgcaggtggt atagtccgga cgtcagggga acctgaccga
     4201 aaggaggtta gttccatgtt ggaacccaag tgggtgactg gcgacccctt caatggagtt
     4261 cagcccgtcg gcttcgtggg gccggtcaag gacgactaga tcgggtcgtt tggacagctc
     4321 tgccgggggc tccgcggttt cgtaccgcgg agccctatcg ctttatatac atgcatggtg
     4381 cggggcggtc ccggaaagag accgccccaa tcacgcccct cgcacctcat cctcgagacg
     4441 gatctctccg tcggtgagaa cctgcgcccg gatcccggca cggcccgcga agttgatcat
     4501 gaacaacgcg ccgtcgcccc gttcgaggag tctcgcgggc cgcatgcacg gctcgcaggg
     4561 ctcaatgccc cgcatccgca ccgagccgac ggaaaaatcc ttgccgacga gcacggtcat
     4621 gtcctcgatc ccggcgatca tgatgttccg tcggatctcg tgcgggccgt aaggcccaat
     4681 gcctcggtgg atgagcgctg cgttcgcctt ggtgtgcgcc tcttcggaga agagggttac
     4741 cccatggcaa acctcgtcgc cgtgcttcct gccgtagtgg taccggtcgc cttcgagacc
     4801 gaggttgaag aacgctcgca ccgcctcgac ggtccgcatc ggatcgccgg gcttcgcggc
     4861 gataaaaaga cctgtcacct tacccatcgt ccgtctccat caagggttgc tgggcggcat
     4921 ggtaccacac gctaaaacgg gccgcccgtg gggcggcccg tggcgcctgc gtgtggattt
     4981 acgcagagcg tcggaattga cggcggcgga tgcgatccac catgacctgc ttgcgcatga
     5041 cggtcaccgt cttaccctgc gcttgccacc aagcttctcc gtactcttcc caaccgagcc
     5101 gaaggtagta gtcgcgagcg cccgcggtgt cgagatagag gtggttgaag ccgtgccggc
     5161 gacaggcgtt ctcggcggtc tccatgagcc ggcggccaac accctggccc cggtactcct
     5221 ccggcacgta gaggccgcag atccagggct ccccatcgat cccgaggtac cacttctcct
     5281 cgatgaccaa catgacgtgc ccagcgacgt cgcctcgatc ggtgaacgcg atgaaaacgt
     5341 cgggcaggct ctcggtgaac gatgggcgga tgtagttcgc agcgatctct tcccttggct
     5401 ccttccaggc ctcggtgagg tagttcagga gctgagcgcg gctgaggagg tcttcctcgg
     5461 cgagccgatc aatggtgatg tacatgtgct gctctcctct gtttcgacac tatcgcactt
     5521 cccttaaaag caaaacgccc cgatcgctcg gggcgttttg ccttttcgat ccttacacca
     5581 ttttcacatc aatcgtgact cccgagggaa ggttgaggtt cgtgagagcc tcgatcacct
     5641 tggggttcgg cgacatgatg tcgatgaggc gcttgtggat gcgcatctcg aactgctcgc
     5701 gcgcatcctt atggacgaaa gtcgagcggt tcacggtcca gcgcttcacc tcggtcggga
     5761 gcgggatggg gccgacgatc ttcgcatcga agcgattcgc cgtatccatg atctgcttca
     5821 ccgagaggtc gaggatcttg tgctcgtagg cgcgcacgcg gatgcgcagc ttggactctg
     5881 cggcagggcc tgcctcagga gccttcttct ttgcgacttt cttggttgcg gttgccatac
     5941 gagtcaaggt gcgatgtgtg aatgatacct tatgcggtga tcttcgtcac cacaccggca
     6001 cccaccgtct tgccgccctc gcgaatcgcg aatcgcatct gctcttccat ggcgatcggg
     6061 gcggtgagct tgaccttgaa ggtcaccgtg tcgcccggca tcaccatctc cttgccgtcc
     6121 ggaagggtca cctcgccggt cacgtccgtg gtgcggacat agaactgcgg cttgtagccc
     6181 gagaagaacg gcgtgtggcg gccaccctcg tctttcgaga ggatgtacac ctctgcctcg
     6241 aactcggtgt gcggggtgat cgagcctggc ttcacgagca cctggccgcg atgcacgtcc
     6301 tccttcttcg ttccgcgaag caggatgccg gcgttgtcgc ctgccatacc ctcctggagc
     6361 tgcttgttga acatctcgat accggtgatg gtcgtcttga cggtgtcctt gaggcccacg
     6421 atctcgactt cctcgccgac cttcgcgatg ccgcggtcga tgcggcccgt cacgacggtg
     6481 ccgcgaccct cgatcgagaa gatgtcctcg atggccatga ggaacggctt gtcggtctcg
     6541 cgaaccggat ccgggaagta ggtgtccatc gtatcgacga gctccttgac cttgagcgcc
     6601 cactcgtcgt ctgccgaggt tgcctcaagc gccttcaggc ccgagccctt gataaccggc
     6661 gtgtttgcgc cgtcgaagcc ctgcttcgta aggagctcgc ggatctcctc ttcaacgagg
     6721 tccaccatgt ccttgtcgtc caccatgtcc accttgttga ggaagacgat gaggcgcggc
     6781 acaccgacct gcttcgcgag caggatgtgc tcgcgggtct
//
LOCUS       DASPKG010000082         5775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_654380, whole genome shotgun sequence.
ACCESSION   DASPKG010000082 DASPKG010000000
VERSION     DASPKG010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654380"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VEA36_03280"
     CDS             complement(<1..383)
                     /locus_tag="VEA36_03280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HYF29358.1"
                     /translation="MERTIFEKIVAREIPANIVYEDEETLAFLDIAPNNPGHTLVIPK
                     AHSRNLLDISEESWLAVMRAVRKLAPIIKEAVGADGINLAMNNEPAAGQIVFHTHVHI
                     IPRHEGDGFTHFPAGEYGEGEASAIA"
     gene            431..2152
                     /gene="recJ"
                     /locus_tag="VEA36_03285"
     CDS             431..2152
                     /gene="recJ"
                     /locus_tag="VEA36_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HYF29359.1"
                     /translation="MFPLHDVLDDTLRDALSAYDDFTAALLARRGITCEEEAEAFLAP
                     SYDEHIHDPMLMTDMPKAAERLARAISTKERITVWSDYDCDGIPGAVVLHDFLKKAGA
                     NFTNYIPHRHDEGYGVNIPGIEKLAKEGTTLVVTVDVGIVDTDAVLRANELGMEVIVT
                     DHHLPSGELPPAYAVVDPKARADEPYPFKELCGGGLAWKLVCATLQHGFDGRDQIPLG
                     WEKWLLDMAGLSTIADMVPLRGENRVIAKYGLFVMRKSPRIGLQRLCKVARVDQRRIT
                     EDDVGFMIAPRVNAASRMGDPRDAFRLFTTTDEGEADELAKKLEAANRSRRAAAGAIT
                     REAHEKLEMRKLEGELPPVIVLGNPEWRPGLLGLVANGIAEEYGRPVFLWGREGNETL
                     KGSCRAGGKVSVNALMHAAPDAFLEFGGHAASGGFSISAEQVPHLQERLVTALAQLPA
                     HTTDESRADQHLAPADVSARLLLSLEKFAPFGMGNEKPAFALHQVQIQEVSWFGKAGE
                     HLRLRILRDEFESETVEAICFYAKRELGVQCLKLVAGERASLLAHLERDTFSRRSPVR
                     LRIVDIK"
     gene            2197..2760
                     /locus_tag="VEA36_03290"
     CDS             2197..2760
                     /locus_tag="VEA36_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HYF29360.1"
                     /translation="MRVIRNVLFLGKPGSGKGTQGIRLAQATHRSHVSSGEYFRTLTA
                     RTDDIGERVRDVLKSGGLVLDPFMTYLLMGQLMGPDSARGTVFDGFGRTPPQAKEFNS
                     VHEWFGWGYLVVLLELPDEVARSRLLGRNAGREDDGSLEQIDRRLRIYEELTAPAIPI
                     FEAAGTLRRIDSNRSIEAVDADIRALL"
     gene            2826..3248
                     /locus_tag="VEA36_03295"
     CDS             2826..3248
                     /locus_tag="VEA36_03295"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HYF29361.1"
                     /translation="MQFTLYADGGARGNPGPAGAGAVVFDSLGKRVVEVSDYLGVATN
                     NIAEYEAVLRGLKKLVEEYPEDILKSAPLTIRMDSKLVIEQLKGAYKVKHPNLVPRYL
                     EVKNLLARSFGAVAFEHVYREKNRDADELANRAMDAGK"
     gene            3289..4731
                     /locus_tag="VEA36_03300"
     CDS             3289..4731
                     /locus_tag="VEA36_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HYF29362.1"
                     /translation="MDRVLLASIVSFACAVALASLQQVPWPAIALSGLVTVLLLLLGR
                     LRRTGAYIAASVVVFFFGLGLARVAFTPSIPEAFLPRFDAKVELVGVVRGDPDIRETT
                     QRVTVEVAQGEDRMRILAVLPLHPELRHGAVVEVSGKLTRPEPFATDGGRIFRYDRFL
                     AKDGIFGMVEFATVEVLAEPAGFDRILGTLYAVKHQFVRGLAAGIPEPHAALAAGLIT
                     GGKQGLGRELLDAFTAAGLVHIVVLSGYNVMIVAEGVLKGLAFLPRRIAASLAAGTIA
                     LFVLAAGAGTASVRAGLMAGLGLFARASGRTYNALRALLFVFFVLLLVNPLLLVYDPG
                     FQFSFAATLGLILGAPILENRLAFIRSTFLRDITATTIAAQLFVLPLLLYQTGNLSLV
                     SFPANIIALPAVPLAMLFSATAGFAGMVVPALAPVLSLPALAFLAYLVWLAETAAALP
                     LATVSLPAFSFLFVPVLYLLLGLLLLRLRR"
     gene            complement(4728..5324)
                     /locus_tag="VEA36_03305"
     CDS             complement(4728..5324)
                     /locus_tag="VEA36_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HYF29363.1"
                     /translation="MTYTPRTFTLPELKGLSKKQIDVHLALYEGYVKHVNLILDTIKQ
                     LRDADAEENAFVINELRRRLGFEFDGMRMHEYYFEQLEEGAVAADEHSPLSQAASERY
                     GNWPSFITHLKEVAGTRGIGWVVTYFDPRANSLHTAFVNDHELGQLAGLPIILAIDLW
                     EHAYMVDYVPAEKKSYVEAFLENVNWDLVNERLAKAAT"
     gene            5422..>5775
                     /locus_tag="VEA36_03310"
     CDS             5422..>5775
                     /locus_tag="VEA36_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012551614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HYF29364.1"
                     /translation="MTTDQAAQRIWDYMKLNQPLEKADAIFVLGSSDLRVPAYAAQLY
                     HEGWAPLIIFSGKEGKLATHRVWGMSEAEKFAEVARELGVPEEAMLLEKEATNTGENV
                     QFTKRLLERKGIAISK"
BASE COUNT          958 a         1794 c         1905 g         1118 t
ORIGIN      
        1 gcgatcgcgc tggcttcgcc ctcgccgtac tcgcccgcag gaaagtgcgt gaagccgtcg
       61 ccctcgtgcc gcgggatgat gtgcacgtgt gtgtggaaca caatctgtcc ggccgccggt
      121 tcgttgttca tcgcgaggtt aatgccgtcg gccccgacgg cttctttgat gatgggcgcg
      181 agcttccgga cagcccgcat aaccgcgagc caggactctt cggagatatc gaggaggttg
      241 cgcgaatggg ccttcgggat gacgagggta tggccgggat tgttcggcgc gatatcgagg
      301 aaggcgaggg tctcctcgtc ctcatacacg atgttcgccg gtatctcccg ggcgacgatc
      361 ttctcgaaga tggtgcgttc catatgccgc acagcatacc gcggactgct tctgatacaa
      421 tctccccacg atgttcccgc tccacgacgt tctcgacgat acgctgcgcg atgcgcttag
      481 cgcctatgac gatttcacgg ccgcgctcct ggcgcgcagg ggcatcacct gcgaggaaga
      541 ggccgaagcg ttcctcgcgc cctcctacga cgaacacatc cacgatccga tgctcatgac
      601 ggatatgccg aaggcggcgg agcgactcgc gcgcgcgatc tccactaagg agcggatcac
      661 cgtctggagc gattacgact gcgacggcat cccgggcgcc gtcgtgttgc acgattttct
      721 caagaaggcc ggcgcgaatt tcacgaacta cattccgcac cggcacgatg aggggtacgg
      781 ggtgaacatc ccgggcatcg agaagctcgc caaggaagga acgacgctcg tcgtcacggt
      841 cgacgtgggc atcgtcgata ccgatgcggt tctccgcgcg aacgaactcg gtatggaggt
      901 gatcgtcacc gaccaccatc ttccttcggg ggagctcccg cctgcgtacg cggtcgtgga
      961 cccgaaggcg cgcgcggacg agccgtatcc cttcaaggag ctctgcggcg gcgggctcgc
     1021 gtggaagctc gtatgcgcta cgctccagca tggctttgac gggcgcgacc agattccgct
     1081 cgggtgggag aagtggctcc tcgacatggc ggggctctcg accatcgcgg acatggtgcc
     1141 gcttcgcggg gagaaccggg tcatcgcgaa gtacgggctc ttcgtgatgc gcaagtcgcc
     1201 gcgcatcggg ctccagcggc tctgcaaggt cgcgcgggtc gaccagcggc gcatcaccga
     1261 ggacgacgtc ggtttcatga tagctccacg ggtgaacgcg gcgtcccgga tgggcgatcc
     1321 gcgcgatgcc ttccgcctct tcactacgac cgatgaggga gaagcggacg agctcgcgaa
     1381 gaagctcgag gccgcgaacc gctcacgacg cgcggccgcc ggcgccatca cgcgcgaggc
     1441 gcatgagaag ctcgagatgc ggaagctcga gggcgaattg cccccggtca tcgtgctcgg
     1501 gaatcccgaa tggcgtccgg gcctgctcgg cctcgttgcc aacggcatcg cggaagagta
     1561 cggccgaccg gtcttcctct ggggccgcga ggggaatgaa acgctgaagg gctcctgccg
     1621 cgccggcggg aaggtgagcg tgaacgcgct catgcatgcg gcgcccgatg cgttcctcga
     1681 gttcggcggg catgccgctt ccggcggctt ctccatctcc gctgagcagg tgccgcatct
     1741 gcaggagcgg ctcgttacgg cgctcgcgca acttcccgca catactaccg atgagtcgcg
     1801 tgcagaccag catcttgcgc ctgcagacgt atcggcgcgg cttcttctct cactcgagaa
     1861 gttcgcgccc tttggaatgg gaaatgagaa acccgcgttc gcgctgcatc aggtgcagat
     1921 ccaggaagtg tcctggttcg gaaaggcagg cgagcacctg cgcctccgga tcctgcggga
     1981 cgaattcgaa tcggagacgg tggaggccat ttgtttctat gcaaaacggg agctcggcgt
     2041 gcagtgtctg aagctcgtcg ccggcgagcg cgcatcgctg cttgcgcacc tcgagcggga
     2101 taccttctcg aggcgttccc ccgtgcggct gcgcatcgtt gacattaagt aggatcttag
     2161 tcatcctgtg cccggatcaa cagggaaggg cccgccatgc gcgtcatcag gaatgtcctg
     2221 ttcctcggga agccgggatc agggaagggc acgcagggaa tccggcttgc ccaagcgacc
     2281 catcggtcgc acgtctcgag cggtgagtac ttccggacgc tcaccgctcg taccgacgac
     2341 atcggcgaac gtgtccgtga cgtcctcaag agtggcggcc tcgtgctcga cccgttcatg
     2401 acctaccttc tcatggggca actcatgggg cccgattctg cgcgcggcac cgttttcgat
     2461 ggattcgggc ggaccccgcc gcaggctaag gagttcaaca gcgtgcatga gtggttcggg
     2521 tggggctatc tcgtcgtgct cctcgagctc cctgacgagg tggcccgatc acggctgctc
     2581 ggccgcaatg cgggccgtga ggatgacggc tcgctcgagc agatcgatcg gcgcctcagg
     2641 atctatgagg agctcaccgc tcccgcgatc ccaatcttcg aggccgcggg aacccttcgg
     2701 cggatcgatt cgaaccgttc gatcgaagcg gtcgacgcgg acatcagggc gctcctctag
     2761 gggaagcgag cagggcgtta cggggcgctc gtccatgacg agcgcccttc tgctatgcta
     2821 ggcccatgca attcaccctc tacgcggacg gtggcgcgcg cgggaatccg gggcccgccg
     2881 gcgccggcgc cgtcgtattc gatagtctcg gcaagcgcgt cgtcgaggtc tctgactatc
     2941 tcggcgttgc gaccaacaac atcgcggaat acgaggcggt tctccggggc ttgaagaagc
     3001 tcgtcgagga atacccggag gatatattga agagtgcacc gctcacgatc cgcatggact
     3061 cgaagctcgt catcgagcaa ttgaagggcg cgtacaaggt gaagcacccg aacctcgtcc
     3121 ctcggtacct cgaggtgaag aacctgctcg cgcgctcctt cggcgccgtc gcgttcgagc
     3181 acgtgtaccg cgagaagaac cgggacgcgg acgagctcgc caaccgcgcg atggacgcgg
     3241 ggaagtagtc tccagctttt tcatattcgg cattgctagg ctcgcggcat ggaccgcgtg
     3301 ctcctcgcct cgatcgtttc gttcgcctgc gcggttgcgc tcgcatcgct tcagcaggtc
     3361 ccctggcccg cgatcgcgct ttccgggctc gtcacggtcc tgctactgct cctcgggaga
     3421 ctgcggcgta ccggcgcgta catcgcggca tcggtggtcg ttttcttctt cggactcgga
     3481 cttgcgcgcg tcgcattcac gccgtccatt cccgaggcgt tcctcccgcg cttcgatgcg
     3541 aaggtcgagc tcgtgggcgt ggtccggggc gatccggaca tccgcgagac cacgcagcgc
     3601 gtgacggttg aggttgcgca gggcgaggac cgcatgcgca tcctcgccgt cctgccgctc
     3661 catccggagc tccggcatgg ggcggtcgtc gaagtgtcgg ggaagctcac gcgccccgaa
     3721 cccttcgcga ccgacggcgg ccgcatcttc cggtacgacc gcttcctcgc caaggatggg
     3781 atattcggga tggtggagtt cgccactgtg gaggtgctcg cggagcccgc agggttcgat
     3841 cgcatattgg gaacgctcta tgcggtgaaa caccagttcg tccggggtct tgcagcgggc
     3901 atcccggagc ctcatgccgc gcttgccgcg ggactcatca cgggcgggaa gcaggggctc
     3961 gggagggagc tcctcgatgc gttcaccgca gcgggcctcg tgcacatcgt ggtcctgtcc
     4021 gggtacaacg tgatgatcgt cgcggaaggg gtattgaagg ggctggcgtt cctgccgcgc
     4081 cggattgcgg cctcgcttgc agcgggcacg atcgcgctct ttgtgcttgc ggcgggcgcg
     4141 ggtactgcaa gcgtgcgcgc ggggctcatg gcggggctcg ggctcttcgc gcgggcctca
     4201 ggccgcacct acaacgcgct gcgcgcgctc ctcttcgtgt tcttcgtgct gctgctcgtg
     4261 aatccgctgc tcttggtcta tgatcctggc ttccagtttt cgttcgccgc taccctcggg
     4321 ctcatcctcg gtgcgcccat cctcgagaat cggctcgcgt tcatacgctc cacgttcctc
     4381 cgggacatca cggcgacgac gatcgcggcg cagctcttcg tgctgcccct attgctgtat
     4441 caaacgggga acctctcgct cgtgtcgttc ccggcgaaca tcattgcgct gccggcggtc
     4501 ccgctcgcga tgctcttctc cgcgaccgca ggattcgccg ggatggtcgt gcccgcgctt
     4561 gcgccggtgc tttcgctccc ggcgctcgcg ttcctcgcct acctggtatg gctggccgag
     4621 acggcggccg cgcttccgct tgcgaccgtc tcgctccccg cgttctcgtt cctcttcgtg
     4681 cccgtgctct atctgctgct tgggctcctg ctgctgcggc tgcgccgcta ggttgcggcc
     4741 ttggcgaggc gttcgttcac gaggtcccag ttcacgttct cgaggaaggc ctcgacgtac
     4801 gacttcttct cggcggggac gtagtcgacc atgtaggcgt gttcccagag gtcgatcgcg
     4861 aggatgatgg gaagccccgc gagctgcccg agctcatggt cgttcacgaa ggcggtatgg
     4921 agcgagttgg ccctcggatc gaagtaggtc acgacccagc cgatgccgcg ggtgccggcg
     4981 acctccttca ggtgcgtgat gaagcttggc cagttgccgt acctctcaga tgccgcctgc
     5041 gaaagcggcg aatgctcatc tgctgcgacc gcgccctcct cgagctgctc gaagtagtac
     5101 tcgtgcatgc gcatgccgtc gaactcgaag ccgaggcggc ggcgcagctc gttgatgacg
     5161 aaggcattct cctccgcgtc cgcatcgcgc agctgcttga tcgtatcgag gatgaggttc
     5221 acatgcttca cgtatccctc gtagagggcg aggtgcacgt cgatctgctt cttcgagagc
     5281 cctttgagct cgggaagggt gaaggtgcgg ggggtgtagg tcatggtaat aggattacgt
     5341 agtgagggca gtataccgca gagcatatgc tcgtgctccg cgtggggagg acggtagaac
     5401 caggggtgct atggtacgga tatgaccact gaccaggctg cacaacgcat ctgggactat
     5461 atgaagctca atcaaccgct tgagaaggcg gatgccatat tcgtcctggg gagcagcgac
     5521 ctgcgcgttc cggcctacgc cgcgcagctc taccacgagg ggtgggcacc gctcattatt
     5581 ttctcaggca aagagggcaa acttgcgacg catagagtgt ggggtatgag cgaggccgag
     5641 aagttcgctg aagtggcacg ggaactcggt gtcccagagg aggcgatgct tctggagaag
     5701 gaggcgacca atacgggaga gaacgtgcag ttcaccaaac gactacttga gaggaaggga
     5761 atagcgatat ccaaa
//
LOCUS       DASPKG010000083        11375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_2011624, whole genome shotgun sequence.
ACCESSION   DASPKG010000083 DASPKG010000000
VERSION     DASPKG010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11375)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11375
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011624"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VEA36_03315"
     CDS             complement(<1..429)
                     /locus_tag="VEA36_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29365.1"
                     /translation="MPRAASFTFGTYHVDTRRSIISFTYRVAFPDGKERTYTDRLFLR
                     DVPPEAWENVPPAVLEPTLQALLLMLGINYWAIFPTRDMRIEGFSLTREQAQFWDALY
                     LNGLGEYFYAMQMDFRDLIAFPYDDAAVAPAAARLTLPARA"
     gene            804..1529
                     /locus_tag="VEA36_03320"
     CDS             804..1529
                     /locus_tag="VEA36_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29366.1"
                     /translation="MRINKTEILLLAAFVLVAAVFYLVSFGAEETDRIFITISTFFFS
                     IFTGFFISRQGSRYSKLRETISTFDGKLSSIYRAAQNVSPEVQKQTGDVIRTHYERTL
                     HDRSWEHHFSDESVIISSIHQVLEDQVGSNKQESLRNQAVGRMLAGLSDAQVLRKNMV
                     MLYQERIPSFQWFLIIFFLVMLLMSISVIPSVGEFLGSILKAAFVISLISVVIILRSL
                     DNLHLFEGFIGENSAKDVLANIK"
     gene            complement(1627..2559)
                     /locus_tag="VEA36_03325"
     CDS             complement(1627..2559)
                     /locus_tag="VEA36_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HYF29367.1"
                     /translation="MQRGIIKHLNFEKGFGFIRVENGPDVFFHASQRHPDELGQPARD
                     REIFFELRQGQKGPVAWQWRLTPPQTPEIDPECDRREGYRPGIMVALRWSGYYFVGQR
                     RGSWAVLMDMMSDPLLEEIRKQTELPIESEADLLAALERPPHPDASKRIDELWTKELN
                     RPDWEFAYGVPQGGIEPGEHWEDAIRRELEEELGDYRKMFGEAQSWSDAIKGQPTFLF
                     KERSNFRVEKDGRVYRGKALYAFLVELNFIPDWVDTMIFGSRGDEHNSFPCPEFENGG
                     EFCDPNEAIQLIRATHKGAKGDQLVRLFEMAKAA"
     gene            complement(2709..3155)
                     /locus_tag="VEA36_03330"
     CDS             complement(2709..3155)
                     /locus_tag="VEA36_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29368.1"
                     /translation="MTAIARTSLEEFIGVTDPMPYGHAISEFQEQVLDAFAGKRLDKA
                     YHVFLYKGKMRADAFMEVHGGKSRGLVEACFQHAELTLRFLRLDHTTQDSFLECILTF
                     FAEHEGVRQLELRVKWGTNSTRTMLVEREDFDGQGGTWTNINFSLW"
     gene            4130..4495
                     /locus_tag="VEA36_03335"
     CDS             4130..4495
                     /locus_tag="VEA36_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HYF29369.1"
                     /translation="MQIHFKGTNYEIAPEISAHARKKLQSVRKILGRHKHNAYAYVDL
                     GKETEAHQNGRIWYADINFEVNGARFYAKATEETLENAVDRAVNELKSELLTARKREQ
                     SLVRKGGVAFKSMLQGFSS"
     gene            complement(4485..5321)
                     /locus_tag="VEA36_03340"
     CDS             complement(4485..5321)
                     /locus_tag="VEA36_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HYF29370.1"
                     /translation="MSFAYFGTPYVARDTLALLVERGFIPAVVVTSPDAPRGRGMALA
                     PSETKAWAQAHGLPVLTPEALTPEVIEELAGYGCTYAIVVAYGKILPQALIDAFPKGL
                     LNIHYSLLPRYRGASPVEAALLNGDAVTGVSIQKMVYKLDAGDLLAQQEVEILPTETT
                     RELRPRLIAIGATLLADILPAFERGELAPTPQDEAQATHCGKIDKREGELDLAGDAAL
                     NWSKYRAYAESPGTYFFVEKGGRRIRVKIVTARFERGQFMPDRVIPEGKKETSYADFA
                     RS"
     gene            complement(5318..5854)
                     /gene="def"
                     /locus_tag="VEA36_03345"
     CDS             complement(5318..5854)
                     /gene="def"
                     /locus_tag="VEA36_03345"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HYF29371.1"
                     /translation="MASILQDGEPVLRQVAKPVPEGLFGTAELKQLLDEMEAALDAEL
                     DGVALAAPQIGIPYRIFIARLDRTVAPKEERRANPEALPAPERVVFINPEFVRSSKRR
                     TQMDEGCLSVRGIYGKTMRHNRATVRARAFDGTTFERGGGGIIAQIYQHEIDHLNGIL
                     FIDHATDLVEMRQEEGTL"
     gene            complement(6327..6620)
                     /locus_tag="VEA36_03350"
     CDS             complement(6327..6620)
                     /locus_tag="VEA36_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29372.1"
                     /translation="MKPIGDFLPPVPEGQMPKKSRAHERGELMRFFVRHLNYARAQDG
                     LPKLTMSRMGKILEGIPTKDLYYLKSVCSRAKHFSKKFWWEVDPKKHEEENPF"
     gene            complement(6625..7932)
                     /locus_tag="VEA36_03355"
     CDS             complement(6625..7932)
                     /locus_tag="VEA36_03355"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HYF29373.1"
                     /translation="MAETGNFIVEGGRPLLGSIVTNRSKNGAVALLAASLLNKGTTTL
                     RNVPKIEEVHRLVEVLRSIGVSCDWSDRDLILQPPAEISLDAIDRAAAMRTRSILMFI
                     GPLLHRFRAFDIPQSGGCSLGLRSVQQHLWALEPFGVAISVLDDRFAVSHDGLAPADI
                     VLYEASDTATINALLAASRIPGRTRIKYASANYQVQEVCGYLRALGIGVEGIGTTTLT
                     VSGSADDIDMPIEYAVAEDPTDAMFFLAAAVTTNSRLTIRRVPIEFLEVELFVLGKMG
                     LRYTLTDAGVSENGITRLADLAIEPSELTAFPEKIHARPYPALNIDNLPFFAVMATQA
                     RGTTLIHDWSYEKRAIYYTELDRLGARTVLMDPHRFQVEGPTPLKGAEIVCPPALRPA
                     TILLIGMLAADGRSTLHNVYTINRGYEDLVPRLQSIGATIERS"
     gene            8000..9013
                     /locus_tag="VEA36_03360"
     CDS             8000..9013
                     /locus_tag="VEA36_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HYF29374.1"
                     /translation="MASFSPRLGIDLGTTNVLVFVPGKGVVLNEPSVVAVSTENNKVL
                     AIGSEAKAMVGRTPDTITAYRPMKDGVIADYRVTEAMLRYFIRKALGRFNLLKPIVLV
                     SVPAGVTSTEKRAVIEAAVKAGAQDAFVVKEPILAAIGAGIPIQEALGRMVADIGGGT
                     IDVAVISLGGIVSSTSVKVAGNKLDRAIVDYLKKQYNLAIGDKTAEEIKIQIGSAVPV
                     AEEISMHVKGRDLVSGLPRTVELKTNEIVQAMNRELREMMNAIRKVLQDTPPELASDI
                     IDNGIILTGGTSQLRNLPELVYRRTGVEAKLADQAYFCVARGTGIALKHLDTYKRSIV
                     SKR"
     gene            complement(9062..9538)
                     /locus_tag="VEA36_03365"
     CDS             complement(9062..9538)
                     /locus_tag="VEA36_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29375.1"
                     /translation="MRAVLFASFFAAALHYGPPALAQPSADLAARSALAQAVRPGLRA
                     PAEFAVAPPVDTRSANPINVRVVPHHDCFAQPFAPQEACEEEREFVLEYKPSTGPDTV
                     FGRLMFRGLKELYEGQRPKKEPRGGPKGPLLPTPDGWEVKGQIVLPLCPVGSGRKC"
     gene            complement(9633..10076)
                     /locus_tag="VEA36_03370"
     CDS             complement(9633..10076)
                     /locus_tag="VEA36_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29376.1"
                     /translation="MNQNVAIGIVVVLALLAGGYLWYQNQGALPLVEEAREAPSETPP
                     SSTAITQGIEGSWRSSADPKFTRSFGAGGTVTDRYEGQADVTGHYELDGDHVPAQVKS
                     LAGSAPVLAIEYPEEAQFFLVTKLTATELELLRVGTTETLRFTRM"
     gene            10171..11121
                     /locus_tag="VEA36_03375"
     CDS             10171..11121
                     /locus_tag="VEA36_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HYF29377.1"
                     /translation="MEFTRAFGGISKDDAHLAGGKGASLGEMTQAGIPVPPGYVVLAS
                     TFERFLEETDLNVEINAILGTVKHDEMHTVEHASEKIQALIHGAQMPEDIATEVRSEF
                     DKLGAEFVAVRSSATAEDSASAAWAGQLDSYLNTTEATLLANVQRCWASLFTPRAIFY
                     RFEKGLDTQKISVAVVVQKMVNSDAAGIAFSVHPVTEDHNQLIIEAGLGLGEAVVSGQ
                     VTPDSYVVTKEPRRIIDVNVSTQSRALYRAAGGGNEWRDIPEPTASAQVLSEAQIMAL
                     SDLVVLIEKHYGFPCDIEWAIEGEGMYIVQSRPITTLSTH"
     gene            11128..>11375
                     /locus_tag="VEA36_03380"
     CDS             11128..>11375
                     /locus_tag="VEA36_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29378.1"
                     /translation="MPIISPKPATKLYKQEGKTGMYQSMLWALSVARARMTHVPVSWG
                     EYFVYFKRNYLGIYWDEVNLEKVAQAVLERAPAGLPDD"
BASE COUNT         2172 a         3381 c         3602 g         2220 t
ORIGIN      
        1 cgctcgcgca gggagcgtga gccgtgcggc cgcaggcgca accgcagcat cgtcgtaggg
       61 gaacgcgatg agatcccgga agtccatctg catggcatag aagtactccc cgagcccatt
      121 gaggtagagc gcgtcccaga actgcgcctg ctcccgggtg agggagaacc cctcgatgcg
      181 catatcccgc gttgggaaga tggcccagta gttgatgccg agcatgagaa gcagcgcctg
      241 gagcgtcggc tcgagcacgg ccggcggtac gttctcccag gcctccgggg gcacgtcgcg
      301 caggaagagc cggtcggtat aggtcctctc cttcccgtcc gggaacgcga ctcggtaggt
      361 aaagctgatg atcgagcggc gcgtatcgac gtggtaggta ccgaaggtaa acgaggcagc
      421 gcggggcatg ttcgtgatca tagcagctcg caacgcctcg tggaggacat tagaaccttt
      481 gtataaaaac ggaagtcgct caacggggag cgcttccgtt aagcgactgc acagacctat
      541 aggacagcgt ccctacgagg tggcgactcc ccatacaaga gctaccgcga ggagaatgat
      601 ggctccaatc atcacaaact ccagaacctt ccccagaaaa gcctcgctag tgaaccgccg
      661 aaacccgcga ttatcggtca tgagactaat cctcttgttt cttacactga ccgaaccata
      721 acattgaatt aataactcgt cagctccgcc tcgtggaggc tgttagaacc ttgggtgtag
      781 aggtgaatgc tacactgtag ctcatgcgta taaacaagac tgagattctg cttttggcgg
      841 cattcgtact ggttgctgcc gtgttttacc tcgtctcttt cggggcagag gaaacggaca
      901 gaatatttat caccattagc accttcttct tctcgatctt cactgggttt ttcatttctc
      961 ggcagggatc tcgctacagc aagcttcggg agaccataag cacttttgat ggaaagctat
     1021 cgagcatata tcgagcagcc cagaatgtga gtcctgaagt gcagaaacaa acgggtgacg
     1081 tcatacgcac gcattacgaa cgcaccttgc atgatcggtc gtgggagcac catttctctg
     1141 atgaatcggt catcatttct tccatacatc aggtgctcga agatcaggtg ggatcaaaca
     1201 agcaagaatc gcttcggaat caggcagtgg gccgcatgct tgcgggtctc tctgacgccc
     1261 aggtgctcag aaagaacatg gttatgctgt atcaggagcg gatcccctca tttcaatggt
     1321 tcctcatcat tttctttctc gtcatgctcc tcatgtcgat cagcgttatc ccttcggttg
     1381 gtgagttcct cggatccatc cttaaggccg cctttgtcat ttcactcatt tctgtggtga
     1441 tcatcttgcg gagcctcgac aatctccacc tctttgaagg ttttatcggt gagaactcgg
     1501 ctaaggatgt gctggctaac atcaagtaac tttcgttagc tccgcctagt tgaggctgtt
     1561 agaacctgtt ttgtataaaa agaagccgcc cgggagcgcg gagatactcc gggcgactcc
     1621 aggcggctag gccgctttgg ccatttcgaa caggcgcacc agctggtctc ccttcgctcc
     1681 cttgtgggtt gcgcggatca gctggatggc ttcgttgggg tcacagaact cgccaccgtt
     1741 ctcgaactcg gggcagggga acgaattgtg ctcgtcgccc cggctgccga agatcatggt
     1801 gtcgacccag tccgggatga agttcagctc cacgaggaag gcgtacaatg ccttgccccg
     1861 atagaccctc ccgtctttct cgacccggaa gttactccgc tccttgaaga ggaacgtagg
     1921 ctggcctttg atggcgtcgc tccagctttg agcctcgccg aacatcttcc tgtagtcccc
     1981 gagctcctcc tcgagttcgc gcctaatggc atcttcccag tgttcgccgg gctcgatgcc
     2041 gccctgcgga acgccgtacg caaattccca gtcggggcga ttcagctctt tggtccagag
     2101 ctcatcgatc cgcttggatg cgtccggatg tggtggccgt tcaagggccg ccagcaagtc
     2161 cgcttcgctc tcgatcggga gctcggtctg tttccgtatc tcctcgagta gcgggtcgct
     2221 catcatgtcc atcaacacgg cccatgagcc ccgacgctga ccgacgaagt agtagccgga
     2281 ccaccgcaac gcgaccatga tgcccgggcg gtagccctct cggcgatcgc attccggatc
     2341 gatttccggc gtctgcggcg gcgtaagacg ccactgccac gcgaccggtc ccttttgtcc
     2401 ctgcctcagt tcgaagaaga tctcgcgatc tcgtgcaggc tgaccaagtt cgtccgggtg
     2461 ccgctgactg gcgtggaaga agacatcggg accgttctcg acacggatga agccgaagcc
     2521 tttttcgaag ttaaggtgct taatgattcc gcgctgcata gcgccctcca ttcctgagtg
     2581 acactattcg cgaatatgaa agcacactat tctatgaaag tcaacgtgct ccgcctagtg
     2641 gaggacgtta gaacttgttt tgtataaaag gttcagggcg cagacgtttc cgtctgcgcc
     2701 cctcctcctc accacagtga gaagttgatg ttggtccagg tgccaccttg gccgtcgaag
     2761 tcttcgcgtt cgacgagcat cgtgcgtgtg gagttcgtgc cccatttcac ccgcagttcg
     2821 agctggcgta caccctcgtg ttccgcgaag aacgtgagta tgcattcgag gaaactgtcc
     2881 tgcgtcgtat ggtcgagacg caggaagcga agggtcagtt ccgcgtgttg aaagcacgct
     2941 tcgaccagtc ctctgctttt gccgccgtgc acctccatga aggcgtcagc cctcattttc
     3001 cccttgtaga ggaacacgtg gtaggccttg tcgagcctct tgcctgcgaa ggcgtcgagg
     3061 acctgttcct ggaactcgct aatggcgtgt ccgtagggca tggggtcggt gaccccgatg
     3121 aattcctcga gggaggtccg ggcaatggcg gtcatttcga ctcgcatggt cgttctcctt
     3181 ttccggattt tcacacgcgg atacccgggt tgcgtgattt ccggaaatgc ttaggtatcc
     3241 tgaaggataa tgtactattt ttatgtattt gtcaaactca ggctccgcgg gtaggattcg
     3301 aacctacgac caatcggtta cacgtgtctt ctcgtctctg aaaagggtgg actatatcat
     3361 cgcccgttcc ccgaagggtt gtgggcggga ggcgcttcca gctccttaga gctgtactcc
     3421 catcacggga tagtctctga accttcaatc ggatcacccg aaagcttggc tgctgattac
     3481 cctcgtcttt ctcgttaggg cttccagaca attcacctcc ttttcatccc tgagtttcct
     3541 cgagggagct gcgtaatgcc atcgtgcact tcggcgtggc agttggcaca aagcaacacg
     3601 catttgtcca actcggtacg catcttctcc cacgatcttg taagtccccg gacagaaaga
     3661 ccgaaacctt tcttcgaggg atcaagatga tggaatacca ttgcgcgccc gcatcgtttg
     3721 tacccacata cgatgcattt gccgccgcca tattcgagag ccatatcacg aagcttcttt
     3781 cgtcgcttca tcactgcctg cttcaaatat tcggcacgat cagcataggt tcgtgtctct
     3841 ggcatacaca tgagtatacc aaggacacac gtaccacagc cgaccgctct accactgagc
     3901 taccgcggaa tatcaatgtc ctctcgcgag gactcaggca ttctagcgga aatccagcat
     3961 tcggcaacgc ttgaattcat gcgaacttcc gcctcgagcg ggtatgtata ggaacgtggc
     4021 ggtactacca cgtgcacata tataaaacga tcacgggacg acggatcgtg cgtttcccat
     4081 agccgcccca ggacctatac tggtgagtag cggataaccc tacacgcata tgcaaataca
     4141 cttcaaaggc accaattatg agatagcgcc cgagataagc gcacatgcga gaaagaagct
     4201 gcagagcgtc cggaagatcc tcgggcggca caagcacaac gcctacgcgt atgtcgacct
     4261 cggcaaggag accgaggcgc accagaacgg gcgcatctgg tatgcggaca tcaatttcga
     4321 ggtgaacggc gcccgcttct atgcgaaagc caccgaagag accttggaga acgccgttga
     4381 ccgggcggtg aacgagctca agagcgagct cctcaccgcg cgcaagcggg agcagagcct
     4441 cgtccggaag ggcggcgtcg cattcaaatc aatgctgcag gggttcagct cctagcgaag
     4501 tccgcgtagc tcgtctcctt cttcccctcc ggtatcacgc gatcgggcat aaactgaccg
     4561 cgctcgaacc gtgcagtaac aatcttcacc cgtatccgcc tcccgccctt ctcgacgaag
     4621 aagtaggtgc cgggcgactc tgcatacgcg cggtacttgc tccagttgag agccgcatcc
     4681 ccggcgagat cgagctcgcc ctcgcgcttg tcgatcttcc cgcaatgcgt cgcctgcgct
     4741 tcgtcctggg gcgtgggcgc gagctcgccc cgctcgaagg cagggaggat gtccgcaagc
     4801 agcgtagccc cgatcgcaat aaggcgtggc cggagctctc gcgtcgtctc agtcggcagt
     4861 atctcgactt cctgctgcgc gagcaggtcg cccgcatcga gcttatagac cattttctgt
     4921 atggaaacgc cggtgaccgc atcgccgttg aggagcgccg cttcgaccgg ggaggcgccg
     4981 cggtacctcg ggaggaggga atagtggatg ttgaggaggc ccttggggaa cgcatcgatc
     5041 agggcctgcg ggaggatctt cccgtacgca acgacgatgg cgtacgtgca gccgtagccc
     5101 gcgagctcct cgatcacctc gggggtgagc gcttcgggag tgaggaccgg gagaccgtgg
     5161 gcctgcgccc acgctttcgt ctccgacggg gcgagcgcca tgccgcgccc gcggggagcg
     5221 tccggcgagg tcacgacgac cgccgggatg aagccgcgtt caacgaggag ggcgagcgta
     5281 tcccgcgcca cgtacggcgt gccgaagtat gcaaagctca tagcgtccct tcctcctgcc
     5341 gcatctccac gagatcggtc gcatgatcga tgaagaggat gccgttcaag tgatcgatct
     5401 cgtgctggta gatctgcgcg atgatgccgc ccccgccgcg ctcgaaggtc gtcccgtcga
     5461 aggcgcgcgc ccggaccgtc gcccggttat ggcgcatggt cttgccgtag atgccgcgga
     5521 ccgataagca cccctcgtcc atctgggtgc ggcgcttcga gctccgcacg aactcgggat
     5581 tgatgaacac gacgcgctcg ggagcaggga gcgcctcggg gttggcgcgc cgctcttctt
     5641 tgggcgccac ggtccggtcc agccgggcga tgaagatgcg gtacgggatg ccgatctgcg
     5701 gggcggcgag cgcgacgccg tcgagctctg catcgagcgc ggcctccatc tcgtcgagca
     5761 gctgcttgag ctccgcggtc ccgaacagcc cctcgggcac gggcttggcc acctgccgga
     5821 gcacgggttc gccgtcttgg agtatggacg ccatatgcag gtacggtagc aagtctgtag
     5881 gcaaaaagaa acgcccgggc atgagccgcg ggcgggtgga gctccatccg gtcggagccg
     5941 gagcgacgat gggtgagggg gtcttcctca cttctggcgg ccggtaccag atatcgtgct
     6001 ctgtctcaaa cgaccggatg gagtccgggg tgcagggacc ggcaagatgc cgtgcccctg
     6061 cggggaaaac ctacctcgta tcgcgaacgc aacggataac cacatctttc tctttcgagt
     6121 gtacctcgat gcggcagggc cgaacgtatc gtgaccgtgt ggtaggcggc gatggaaaat
     6181 aacatgcgcg ggaccgatgc agcgtctggg gttggagggt agacgctatc cgtgtgggga
     6241 cacgacactg gtcccgcgcc ggccttcggg gaacgggaac ccctaggccc tgggagaagt
     6301 aaaccaccgt ccggcagagc cgtcaatcag aaggggttct cctcttcatg tttcttcggg
     6361 tccacctccc accagaactt cttgctgaag tgcttggcgc ggctgcacac ggacttaagg
     6421 taatagaggt ccttcgtggg gatgccctcg aggatcttgc ccatgcggga catggtgagc
     6481 ttgggaagcc cgtcctgcgc gcgggcgtag ttgaggtgcc ggacgaagaa gcgcatgagt
     6541 tcgccccgct cgtgggcccg gcttttcttc ggcatctggc cctccgggac cggggggagg
     6601 aaatcgccga tcggcttcat atccctagga gcgctcgatc gtcgcgccga tggattgcaa
     6661 ccgcggcacg aggtcctcgt agccgcggtt gatggtgtag acgttatgca gggtcgagcg
     6721 cccgtcggca gcgagcatgc caatgaggag gatggtcgcg ggccggagcg ccggcgggca
     6781 gacgatctcc gcgcccttga gcggggtcgg gccctccacc tggaagcggt gcggatccat
     6841 gagcacggtg cgggcgccga ggcgatcgag ctcggtgtag tagatggcgc gcttctcgta
     6901 ggaccagtcg tggatcagcg tcgtgccgcg cgcctgcgtc gccatgacgg cgaagaaggg
     6961 gagattatcg atattgagcg cggggtaggg acgcgcatgg atcttctcgg ggaaagcggt
     7021 cagctccgac ggctcgatcg cgaggtccgc caggcgcgtg attccgttct cggagacgcc
     7081 ggcatcggtc agggtgtacc ggaggcccat cttgccgagc acgaagagct cgacctcgag
     7141 gaactcgatc ggcacgcggc ggatggtcag tcgggaattg gtcgtgaccg cggccgcaag
     7201 gaagaacatc gcgtcggtgg ggtcctccgc gaccgcgtac tcgatcggca tatcgatatc
     7261 gtccgcgctc cccgataccg tgagcgtcgt ggtaccgatg ccctccacac cgatgccgag
     7321 cgcgcgcagg tacccgcaga cctcctgcac ctggtagttc gccgaagcgt atttgatccg
     7381 cgttcgcccg gggatgcggg aggccgcaag gagcgcattg atggtggcgg tatcgctcgc
     7441 ctcgtagagg acgatgtcgg cgggggcgag cccgtcgtgg ctcacggcga agcggtcgtc
     7501 gagcacgctg atcgcgacgc cgaagggctc gagcgcccag agatgctgct gcacgctgcg
     7561 cagcccgagc gagcagccgc ccgactgcgg gatgtcgaag gcgcggaacc gatggagcag
     7621 gggcccgatg aacatcagga tgctgcgggt gcgcatggcc gctgcccggt cgatggcatc
     7681 gagcgagatc tcggcgggcg gctggaggat gaggtcccgg tcgctccaat cgcaggagac
     7741 gccgatggag cggagcacct cgacgaggcg gtgcacctcc tcgatcttcg gcacgttcct
     7801 gagggtggtc gttcctttat taaggaggga ggcggccagg agcgccaccg cgccgttctt
     7861 cgagcgattg gtcacgatcg agcccaatag gggacgcccg ccctcgacga tgaagttgcc
     7921 ggtctccgcc atggtgcggt cattgtacca gagccgtatt ttgaggcgga gcggtgagcg
     7981 ggtatgatac ccgagcgtta tggcttcatt ctcaccgcgt cttggcatcg atcttggcac
     8041 cacgaacgtg ctcgtgttcg tgccggggaa gggggtggta ttgaacgagc cctcggtcgt
     8101 cgcggtctcg accgagaaca acaaggtgct cgcgatcggc agcgaggcca aggcgatggt
     8161 ggggaggacg cccgacacca tcactgccta ccgccccatg aaggacgggg tcatcgctga
     8221 ctaccgcgtc accgaggcca tgctccggta cttcatccgc aaggcgctcg gccggttcaa
     8281 tctcctgaag cccatcgtgc tcgtgtcggt gcctgcgggc gtgacctcga ccgagaagcg
     8341 cgcagtcatc gaggcggcgg tgaaagctgg cgcgcaggac gccttcgtgg tgaaggagcc
     8401 catcctcgcg gcgatcggcg cgggcatccc catccaggaa gcgctcgggc gcatggtcgc
     8461 ggacatcggc ggcggcacca tcgacgtcgc ggtgatctcg ttaggcggca tcgtctcctc
     8521 gacctcggtg aaggtggcgg ggaacaagct cgaccgcgcc atcgtcgact atttgaagaa
     8581 gcagtacaac ctcgcgatcg gggacaagac cgcagaggag atcaagatac agatcgggtc
     8641 ggcggtgccg gtcgccgagg agatttccat gcacgtgaag ggccgggacc tcgtgtcggg
     8701 actgccgagg acggtggaac tcaagacgaa cgagatcgtc caggcgatga accgggagct
     8761 ccgcgagatg atgaacgcga tccgcaaggt gctgcaggat acgccgcccg agctcgcctc
     8821 cgacatcatc gataacggca tcatcctcac gggcgggacg agccagctcc ggaaccttcc
     8881 ggaattggtg taccgtcgaa ccggcgtgga ggccaagctc gcggaccagg cgtacttctg
     8941 cgtcgcgcgg gggaccggca tcgcgctcaa gcatttggat acgtacaagc ggagcatcgt
     9001 ctctaagcgg taattcgaaa gggcctgcgc ttgcgcgcag gcccccgaac gtttgtgctc
     9061 ttcagcactt cctgccgctg ccgaccgggc agagcgggag cacgatctgg cccttcacct
     9121 cccacccgtc cggagtcggg aggagcggtc ccttcgggcc gccgcggggc tccttcttcg
     9181 ggcgttggcc ctcgtagagc tccttgaggc cgcggaacat gagccggccg aagaccgtgt
     9241 ccgggcccgt ggacggcttg tactcgagga cgaactcacg ttcctcctcg caggcctcct
     9301 gcggcgcgaa cggctgggcg aagcaatcgt ggtgcgggac gacccgcaca ttgatcgggt
     9361 tcgctgaccg cgtgtcgacc ggcggcgcca ccgcgaactc tgcgggcgcg cgaaggccgg
     9421 gacgcaccgc ctgcgcaagg gcgcttctag cggcgagatc cgctgacggc tgagctaggg
     9481 cgggagggcc atagtgcagc gcggcggcga agaaggaagc gaagagaact gcgcgcatct
     9541 atggaccctc cagttggagc gcgaccgccg ggttggcgtg tctgtagcaa gtatcgctct
     9601 ggtacagaac ttgtcaagtg tcaagggtgc gcttacatcc gggtgaagcg aagcgtctcg
     9661 gtcgtgccca cccgcaggag ctcgagctcg gtggcggtca gttttgtcac gaggaagaac
     9721 tgtgcctcct ctgggtactc gatcgcgagc accggcgcgg agccggcgag gctcttcacc
     9781 tgggcgggaa cgtggtcccc atcgagctcg tagtgcccgg tgacgtccgc ctggccttcg
     9841 tagcgatcgg ttacggtgcc gcccgcgccg aacgagcgag tgaactttgg atccgctgaa
     9901 ctgcgccagc ttccctcgat gccctgcgtg atcgcggtcg aggagggcgg ggtctcggac
     9961 ggagcctccc gcgcctcctc gacaagcggg agcgctccct ggttctgata ccagaggtac
    10021 ccgcccgcaa ggagcgcgag gaccacgacg ataccgatgg cgacgttctg attcataggg
    10081 atggattaga ggatatgcat aaagtgtaca cccctgccgc accccttcat gaagctcgac
    10141 aaatctgcta tggtgtggct atacagggcc atggaattca cccgcgcatt cggggggata
    10201 tcgaaggacg acgcgcacct cgccggcggg aaaggtgcgt ccctcgggga gatgacccag
    10261 gcgggtatcc cggtcccacc ggggtacgtc gtgctcgcct ccaccttcga gcggttcctc
    10321 gaggagacgg acctgaacgt ggagataaac gccatcctcg gcaccgtgaa gcacgacgag
    10381 atgcataccg tggagcatgc ctcagagaag atccaggcct tgatccatgg cgcgcagatg
    10441 cccgaggata tcgcgacgga ggtcaggagc gagttcgata agctcggcgc cgagttcgtc
    10501 gcggtccgct cgtccgcgac cgccgaagac agcgcgtcgg cggcctgggc cgggcagctc
    10561 gattcctatt tgaatacgac tgaggcgacc ctgcttgcga acgtgcagcg ctgctgggcg
    10621 tctttgttca cccctcgcgc catcttctac cgcttcgaga aggggctcga tacccagaag
    10681 atctcggtcg cggtcgtggt gcagaagatg gtgaactcgg atgcggccgg gatcgcgttc
    10741 tcggtgcatc ccgtgaccga ggaccacaac cagctcatca ttgaggcggg gctggggctc
    10801 ggggaggcgg tggtctcggg gcaggtgacg cccgactcgt acgtggtgac gaaggagccg
    10861 cgcaggatca tcgatgtgaa cgtgagcacg cagagccgcg cgctgtaccg ggcggccggc
    10921 ggcgggaacg agtggcgaga cattccggaa cccaccgcct ccgcgcaggt gctctccgag
    10981 gcgcagatca tggcgctctc ggatctcgtc gtgctcattg agaagcacta cggcttcccc
    11041 tgcgatatcg agtgggccat tgagggggag ggtatgtaca ttgtgcagag tcggccgatc
    11101 acgacgctct cgactcattg atcatccatg ccgatcatct cgcccaagcc cgccacgaag
    11161 ctctataagc aggaagggaa gaccggcatg taccagagca tgctctgggc gctctcggtc
    11221 gcgcgggcgc gcatgaccca cgtaccggtt agctggggcg aatatttcgt gtacttcaag
    11281 cgcaactacc tcggcatcta ttgggacgag gtgaacctcg agaaggtggc gcaggcggtc
    11341 ctcgagcgtg cgcccgcagg gctcccggac gactg
//
LOCUS       DASPKG010000084         5740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1199125, whole genome shotgun sequence.
ACCESSION   DASPKG010000084 DASPKG010000000
VERSION     DASPKG010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199125"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..737)
                     /locus_tag="VEA36_03385"
     CDS             complement(291..737)
                     /locus_tag="VEA36_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29379.1"
                     /translation="MRRGIRIRLRLLSGILIVFAALIVLRLYFVQVVHGADYALRAEK
                     QYISASQELFNRGTIYFTRKDGSLLSAATLSTGFTIAMNPEQVVDAEEAYTALAAHIP
                     VDRAEFLTLAAKEDDPYEVLARRVPEEVGEAIEQAKIPGIRVERER"
     gene            complement(748..1026)
                     /locus_tag="VEA36_03390"
     CDS             complement(748..1026)
                     /locus_tag="VEA36_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29380.1"
                     /translation="MPDRTISILSLALGILVFAYLSLMVVTVSLAAWRTDLAAEVRDT
                     EVRIAQLEQRYYDEIERIGATDPAVVGLAKPSRVTYATMVEAPSVTRR"
     gene            complement(1019..1957)
                     /gene="rsmH"
                     /locus_tag="VEA36_03395"
     CDS             complement(1019..1957)
                     /gene="rsmH"
                     /locus_tag="VEA36_03395"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HYF29381.1"
                     /translation="MEAKHDSVLLHEAIEALSVSPADTVVDATIGGAGHFRVLLEKLG
                     PEGTLIGIDADADAIERAKEAATGATPRVELVHDNFRNLASVLEGKGIASIDRALFDL
                     GWSGFHLASGRGFSFKAEEPLLMTYGDPVDTMSAADLVNDLSEESLADLIYTLGEERF
                     SRGIARSIVAAREKDRIETTTQLVAAIEAGVPGWYRARRIHPATKTFQALRIAVNDEL
                     GALREGLSAALASLSDGGRVALITFHSIEDRIVKQMLREAAHDGMGTVVTKKPIVPTS
                     DELAANPRARSAKLRVFERSPRASTYMPRSTFALYA"
     gene            complement(1962..2396)
                     /gene="mraZ"
                     /locus_tag="VEA36_03400"
     CDS             complement(1962..2396)
                     /gene="mraZ"
                     /locus_tag="VEA36_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HYF29382.1"
                     /translation="MFIGEYRHTFDAKNRISLPAKFRKELGGSVIVTRGLDHCLFVYP
                     KTAWKKQAEKFAAYSTGSAAGRGLSRLMLAGASEADLDSAGRILVPDYLKSFAKLGVK
                     SVVAGVSDRVEIWDEKAWEIYTKSIERDADEFAERLGSAGAL"
     gene            2775..3272
                     /locus_tag="VEA36_03405"
     CDS             2775..3272
                     /locus_tag="VEA36_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29383.1"
                     /translation="MQNSREHSRAKIVVYGGVPEAVIARHVLSELGGNVIELSDFERD
                     PRAAYGLFFVDGPFKREGHRGDSRPCDGLLRVRTDAEELRRVGITTMPQILAPALGFI
                     VCRRLTTSELNDKEMRVRFTDALRQGERVCTRRWRALTQPKGAPLRAAPKRLLKVGGM
                     ERSFC"
     gene            complement(3348..3815)
                     /gene="greA"
                     /locus_tag="VEA36_03410"
     CDS             complement(3348..3815)
                     /gene="greA"
                     /locus_tag="VEA36_03410"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HYF29384.1"
                     /translation="MSEQETLVSQEKFDELLSELDTLKTVRRSEIAKNLEYARSLGDL
                     SENAEYQEARELQAATEERIRKLEELVKRAKIATDNKKKDVVGFGSTVTIRKEGGESH
                     AYTIVGSEEADMRVHKLSHVSPLGAALMGKKKGDIFTFETPAGKQTYTVEKVG"
     gene            complement(3875..5551)
                     /locus_tag="VEA36_03415"
     CDS             complement(3875..5551)
                     /locus_tag="VEA36_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HYF29385.1"
                     /translation="MTGRRFGRRRGEREIAPDEIFLDSSNLPEFNQGRLEGKLEKPVS
                     RAAYAGILLAVALTFFGLIAQAANLQIREGERYALQSERNRVRPEVMFAKRGAIVDRN
                     GNPLVYNDESEEGFVKRHYESPGFGHLLGYVSYPKKDSSGNYYDTEIKGLAGVEAAFN
                     DVLAGRNGTLLIEENALGEVQSQGQVAAAKDGATLTLSIDARAQKAFASSIAELAQRI
                     PFTGAAAILMDVETGEIHALASYPEYDPNILSSGGPGEVIAGYQNDSRRPYLDRAIAG
                     LYAPGSIVKPMEAAGALHDGIIDPEKTIFSSGQLVVPNPYDPSKPTIFKDWKALGAMN
                     MRSAIAYSSDVYFYTVGGGFGDQRGLGIKRLGEWFRAFGFDTPTGIELEGEAEGFIPS
                     PEWKERTYDEPWRIGNTYHTAIGQYAMQITPIEAARAVAAVANGGKLLRPTLLKSSMT
                     PAGERIDISPYALQVAREGMRMGVTEGTSVGLSALSFTTIAGKTGTAQLGANNEYHHA
                     WAIGFFPYEKPKYAYVVLMEKGPAGNVIGGIYATYHALSRLHQAAPEYFE"
     gene            complement(5548..>5740)
                     /locus_tag="VEA36_03420"
     CDS             complement(5548..>5740)
                     /locus_tag="VEA36_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29386.1"
                     /translation="AAAFALIAALFTPPVALLFGVLVDLIYFVPGAYPVPLGTILGLA
                     GFMLAELVHGFVKTRIMSV"
BASE COUNT          985 a         1806 c         1875 g         1074 t
ORIGIN      
        1 gagccgtagc gcccattcac ctccaagagg acctgctgga gcttttcctg gaccatgggc
       61 tcgatggtgg tcacaaggtc cccctcgcgg gccgcccggg catccaccat gacgctgtcg
      121 aggttggcga acaattccgc gaagaagttc ccgaagagcc cctcgttctc ccgggagagg
      181 acgtcctcgt agtagcgctc gaggccgtag cgcccctcga tctcgttgtc gttatcgtag
      241 gcgacgaacc cgatcgattg cgcggcctgg gtgccggccg gatagctccg ctatcgctcg
      301 cgctccacgc ggatgccggg aatcttggcc tgctcgatcg cctcgcccac ctcctcgggg
      361 acgcgcctcg cgagcacctc gtacggatcg tcctccttcg cggcaagcgt aaggaactcg
      421 gcccgatcga ccgggatatg ggcggcaagg gcggtgtacg cctcctccgc gtcgacgacc
      481 tgctccgggt tcatcgcgat ggtgaagccg gtggagaggg tcgcggcgga cagcagcgac
      541 ccgtccttcc gggtgaagta gatggtgccg cggttgaaga gctcctggct cgcggagatg
      601 tactgcttct cggcccgcag cgcgtagtcg gcgccatgga ccacctgcac gaaatagagc
      661 cgcaggacaa tcaaggcggc gaagacgatg aggatgcccg agaggaggcg gaggcgtatg
      721 cggatcccgc ggcgcatact cccgattcta ccgccttgtg accgaaggcg cttcgaccat
      781 ggtcgcgtag gtgacgcgtg agggcttcgc gagccctacg accgcgggat cggtcgcgcc
      841 gatgcgctcg atctcgtcgt agtagcgctg ctcgagctgc gcaatccgca cctcggtatc
      901 gcggacctcg gcagcgagat cggtccgcca tgcggcaagc gacaccgtca cgaccatgag
      961 cgataggtac gcgaatacca ggatgccaag cgcaagcgac aggatggaaa tggtgcggtc
     1021 aggcatagag ggcgaaggtg gagcgcggca tataggtcga agcgcgcggc gagcgctcga
     1081 agacccgcag cttggcgctc cgtgcgcgcg ggtttgccgc aagctcgtct gaggtaggca
     1141 cgatgggctt cttggtgacg acggtcccca tcccgtcatg ggccgcctcg cggagcatct
     1201 gcttcacgat gcggtcctcg atggagtgga aggtgatgag cgcgacccgg ccgccgtcgg
     1261 acaaggaagc gagcgcggcg gaaaggccct cgcgcagcgc accaagctcg tcgttcactg
     1321 cgatgcgcag cgcctggaag gtcttcgtcg ctggatggat gcgccgcgcg cggtaccatc
     1381 cggggacacc tgcctcgatc gccgcaacga gctgggtcgt cgtctcgatg cgatcctttt
     1441 cgcgcgccgc gacgatcgag cgcgcgatgc cgcgcgagaa ccgctcctcc ccgagggtgt
     1501 agatgaggtc ggcgagcgat tcctcggaga gatcgttcac gaggtccgca gcgctcatgg
     1561 tgtcgaccgg gtccccatag gtcatgagta gcggctcctc ggccttgaag gagaagccgc
     1621 ggccgcttgc gagatggaat ccggaccagc cgaggtcaaa gagggcccgg tcgatcgagg
     1681 cgatgccctt cccttcgagc acgctcgcga ggttgcggaa gttgtcgtgg acgagctcga
     1741 cgcggggcgt tgcgcccgtt gcagcctcct tggcgcgctc gatcgcgtcc gcgtccgcgt
     1801 cgatgccgat caaggtcccc tcagggccga gcttctcgag gaggacccgg aagtgccctg
     1861 ccccgccgat ggtggcgtcc accacggtat cagccgggga taccgaaagc gcttcgatgg
     1921 cctcatgcaa caggacgctg tcgtgcttcg cttccatacg attagagggc gcctgcgctc
     1981 ccgaggcgct ccgcgaactc gtcggcatcg cgctcgatgg acttggtgta gatctcccag
     2041 gccttctcgt cccagatctc gacgcggtcc gagacgccgg cgacgaccga cttcaccccg
     2101 agcttggcga aggacttgag gtaatccggc acgaggatgc gcccggcgct gtcgaggtcg
     2161 gcttcggaag cgcccgcgag catgaggcgg gagaggccgc gtcctgcggc gctcccggtc
     2221 gagtatgcgg cgaacttctc ggcctgcttc ttccatgcgg ttttcggata cacgaagagg
     2281 cagtggtcga gaccgcgggt cacgatgacg cttccaccga gctccttccg gaacttcgcc
     2341 ggaagtgaga tgcggttctt tgcatcgaag gtgtgacggt attcgccgat gaacatgtaa
     2401 aaaatggatt ccagtgggct tcggtgagcg atggaagaag aggaaaaacc atcctcccac
     2461 ttcatcccac tagtatccaa gtatatgaca ctttgcccca ctgcatgggt cgttatcccc
     2521 aggaatttat tgctcagttt gactcctcgc ctcggcgcgc tatcatgatt cctacatgaa
     2581 cttcttctcg cgttggatcc tcggcatctt tatctacttc ggccttctag aaccggtcgg
     2641 ggtctcgctt gaggtttctg agtaaataga ctagaaactt gaaggaaggc cctgaccaaa
     2701 agtcagggtc cttcacgttt tggggcgacg aacagtcctt ttcaccgccg ataacggcaa
     2761 ttagggagtg agagatgcag aattcacgtg agcattcgcg tgcgaagatc gtcgtgtacg
     2821 gcggcgtccc cgaagccgtc atcgcccgcc acgtcctcag cgaattggga ggcaacgtca
     2881 tcgagctctc ggacttcgag agggacccca gggcggcata cggcctgttc ttcgtcgacg
     2941 gccccttcaa acgggaaggc catcggggcg actcccggcc gtgcgacggc ctgctcaggg
     3001 tccgtacgga tgccgaggag ctccggcggg tcggcataac gaccatgccg cagatcctcg
     3061 ctccggcgct cggcttcatc gtctgccggc ggctcacgac cagtgagctc aacgacaagg
     3121 agatgcgagt gcgcttcacc gacgcgctgc ggcagggaga acgggtctgc acacgccggt
     3181 ggcgggccct cacccagccg aagggagcgc cactgcgggc ggcgccgaag cggttgctga
     3241 aggtcggcgg catggagcgc agcttctgct aacgggcgcg ggcggaaata cgaatcgggg
     3301 cggcccacgc agggccgccc ctcgcttatt tatataaggc agaccgacta ccctaccttc
     3361 tccaccgtat acgtctgctt gcctgctggc gtctcgaagg tgaagatgtc gcccttcttc
     3421 ttgcccatga gggcagcgcc caagggagag acgtgcgaga gcttgtgcac gcgcatatcc
     3481 gcctcctcgc ttcccacgat ggtgtaggcg tggctctcgc cgccctcctt gcggatggtg
     3541 acggtcgagc cgaagcccac cacgtccttc ttcttgttgt cggtcgcgat cttcgcccgc
     3601 ttcacgagct cctcgagctt gcggatgcgc tcctcggtcg cggcctggag ttcccgggcc
     3661 tcctggtatt ccgcgttctc ggagaggtcg ccgagcgaac gggcatactc gagattcttc
     3721 gcgatctcgg aacggcgcac ggtcttcagg gtatcgagtt cactgaggag ttcatcgaac
     3781 ttctcctggc taacgagggt ctcttgctca ctcatgatgt gcgggaactg aatgggtagt
     3841 ggccccattg tacgtgaata ggggtggggg tcaattattc gaaatactcg ggggccgcct
     3901 ggtggagcct gctcaaggcg tggtacgtgg cgtagatgcc gccgatgacg ttcccggcag
     3961 gccccttctc catgagcacg acgtacgcat acttgggctt ctcgtacggg aagaagccga
     4021 tggcccaggc gtggtggtac tcgttgttcg cgccaagctg ggccgtgccg gtcttgccgg
     4081 cgatggtcgt gaaggagagt gcactcagcc ccaccgacgt tccctcggta acccccatcc
     4141 gcatgccctc acgggccacc tggagcgcgt aggggctgat atcgatgcgc tccccggcag
     4201 gcgtcatgga ggacttcagg agggtgggac gcaggagctt gccaccgttt gcgaccgcgg
     4261 cgaccgcacg cgccgcttcg atcggcgtta tctgcatggc gtactggccg atcgcggtgt
     4321 ggtacgtgtt cccgatccgc cacggctcgt cgtaggtccg ttccttccat tcggggctcg
     4381 gaatgaagcc ctccgcctcc ccttcgagct cgatgccggt cggggtatcg aacccgaagg
     4441 cgcggaacca ttcgccgagc cgtttaatgc cgagcccgcg ctggtccccg aacccgccgc
     4501 ccaccgtata gaaatagacg tcggaggagt aggcgatggc gctccgcatg ttcatggcgc
     4561 cgagcgcctt ccagtccttg aagatggtcg gctttgaggg gtcgtacggg ttcgggacga
     4621 cgagctgccc cgaggagaag atggtcttct cgggatcaat gatgccgtcg tggagcgctc
     4681 ctgccgcctc catcggcttc acgatggagc ctggggcgta gagcccggct atggcgcgat
     4741 cgaggtaggg acgcctcgaa tcgttctggt atcccgcgat cacctcgccg gggcccccgg
     4801 aggagaggat gtttggatcg tactcggggt aggacgcgag cgcatgtatc tcccccgtct
     4861 ccacgtccat gaggatggcc gccgctccgg tgaacggaat gcgctgcgca agctccgcga
     4921 tcgaggaggc gaaggccttc tgcgcgcgcg cgtctatgga cagggtcagc gtcgcgccgt
     4981 ctttggcggc cgccacctgc ccctggctct gcacctcccc gagcgcgttc tcctcgataa
     5041 gcagcgtgcc gtttctcccc gcgagtacat cgttgaaggc cgcctcgacg cccgcgagcc
     5101 ccttgatctc ggtatcgtag tagttgccgg aggagtcctt cttcgggtag gagacgtacc
     5161 ccaagaggtg tccaaacccc ggcgactcgt agtggcgctt cacgaacccc tcctcgcttt
     5221 cgtcgttgta gacgagcgga ttcccgttgc ggtccacgat ggcgccgcgc ttggcgaaca
     5281 tcacctccgg gcgcacccgg ttcctctcgc tctggagcgc gtaccgctcc ccttccctaa
     5341 tctggaggtt tgcggcctgc gcgatgagcc cgaagaaggt cagcgccacc gcaagcagga
     5401 tcccggcgta cgccgcgcgt gacaccggct tctcgagctt cccttcgagg cgtccctggt
     5461 tgaattcagg aagattggag gagtcgagga agatctcgtc cggcgcgatc tcgcgctccc
     5521 ctcgcctcct gccgaaccgc ctccccgtca tacggacatg atacgcgtct tcacgaagcc
     5581 gtgcacgagt tccgcgagca tgaagcccgc aagcccgagg atggtgccga gcggaacggg
     5641 atacgcccca ggtacgaaat agatgaggtc gacgagcacc ccgaagagga gggcgacggg
     5701 cggggtgaag agggctgcga tgagcgcaaa ggcggcagcc
//
LOCUS       DASPKG010000085         4356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_657285, whole genome shotgun sequence.
ACCESSION   DASPKG010000085 DASPKG010000000
VERSION     DASPKG010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657285"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VEA36_03425"
     CDS             <1..599
                     /locus_tag="VEA36_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29387.1"
                     /translation="PTGRPEWLDMRDPNVASAVFATHGASGVQEIVDASLGECSDITE
                     LDTLINDLDNATKVHEADTKKIRKDQPLHQTWQGVLTVADGATQVVPGISVGGPLGTG
                     YAIGGGIRTVKQLGNENWQGRLDRNYLSGARLQVKGMQVFLASERIYHRHMKVYCPVL
                     SGYVKKFGAQISFTPVATTVTTPQPGPTAPSPSDWNQP"
     gene            complement(669..1166)
                     /locus_tag="VEA36_03430"
     CDS             complement(669..1166)
                     /locus_tag="VEA36_03430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HYF29388.1"
                     /translation="MNVTGTGIAVGLAVVIALGFLYFGPAVFTPFAPAEPVALEQSAT
                     TTMQSFLDPNQPLPTELAMRDVVVGTGAEAAAGKQVTVNYIGMLPDGTVFDASDRHGR
                     PFTFTLGAGEVIPGWDQGVAGMKVGGKRQLVIPPALAYGERGAGGVIPPNATLLFEVE
                     LVEVR"
     gene            complement(1224..2987)
                     /locus_tag="VEA36_03435"
     CDS             complement(1224..2987)
                     /locus_tag="VEA36_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29389.1"
                     /translation="MSNAEQGPKKAQPKTESPPQGTRVPEAEQQRAAELIAQARADWQ
                     SFVDQVQAAGISKEEISEDWKRAYDAHDAIVRNLSRITDEPGSKRREALLKRHEKLSK
                     ESAKAYANVVGADRARGSDGDQEKIAAIRNRLGGKEKDVAQPAPALEEQLGALERASK
                     RQDGPEPDPKPETHAEHEEQLAEHIARLEYDEGVEAKQPEPGLAYDEGGVWGQFSDEM
                     EQHRQAGRAPQEPKPKLEPVPTPIAQAPEATPAPEAAPAEEPRAVMVNSVLTRQAMGW
                     NSWDKKRLAKFGPFPADPRYSDTAAPAPNAAISEKRPWWKPGKRTAWGAGLGGAGLAA
                     GLFAAIFANQGSDQERSTVKASTGPAAAAKNAEDAGVARATADAPESITLPYDPMQWP
                     AGAQIQSLREWAGTGATTNPAAEAPAQEAAQEVEKEVKKKAPKVRKHRSITPQQHKAW
                     ERIFGLYAATYYPKGYRPEDEKKVRERMVKELGYPNGTFFDGKRAAWSPVPEPAPAAA
                     SPEAQAVPPIAAKPRPFSSESVTAPVAKPRPFSAEPAYDAAPFETTVTMTRPEPKELT
                     DEEIRKAFREQLGRPKAREFK"
     gene            complement(2993..>4356)
                     /locus_tag="VEA36_03440"
     CDS             complement(2993..>4356)
                     /locus_tag="VEA36_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HYF29390.1"
                     /translation="KGIESAPHLTQARFTMAAAPLELSKTGILFGTNAFRGATTEVRL
                     SPEDRLRHLYVIGQTGTGKTSFMKTLIEQDIKAGNGVCFIDPHGNDILDVLASVPPER
                     YDDVIYFDPAYLDRPLGLNLLEYDPAHPEQKTFIVNELLAIFRRLYGDVPESMGPAFE
                     QYFRNATLLVMEDPSSGSTLMDIGRVLSNASFRNLKLAKSMNPVVNQFWTEIATQAEG
                     EASLANIVPYITNKFDDFTANDFMRPIIGQQESSFKFREVIDNKKILLVNLSKGRLGE
                     RNANLLGLILVGKLFMAALSRADNPRGDFPPFYLYIDEFQNITTDSIPGILSEARKYK
                     LSLTIAHQFLQQVDEKIRNAVFGNVGSMAVFRVGEEDGEYFAKQFAPVFSALDFVNIE
                     NYNAYARLLANGVPQKPFNFKTLPPSAGNPGQIDDLRQLSYLTYGRDRGTVESMIRSR
                     YLT"
BASE COUNT          689 a         1411 c         1420 g          836 t
ORIGIN      
        1 cgcccacggg acggccggaa tggctcgaca tgcgcgaccc gaacgtggcc tcggccgtct
       61 tcgcgaccca tggcgccagc ggcgtccagg agatcgtcga cgccagcctc ggcgaatgct
      121 cggacatcac cgagctggat acgctgatca acgacctcga caacgccacc aaggtgcacg
      181 aggccgacac caagaagatc cggaaggacc agccgctgca ccagacctgg cagggcgtcc
      241 tgaccgtcgc tgacggcgcc acgcaggtgg tgcccggcat cagcgtcggc gggcccctcg
      301 ggaccggcta cgcgatcggc ggcggcatcc gcaccgtgaa gcagctcggc aacgagaact
      361 ggcagggccg gctcgaccgg aactacctga gcggcgctcg gctgcaggtc aaggggatgc
      421 aagtcttcct ggcctcggag cggatctatc accggcatat gaaggtgtac tgccccgtcc
      481 tcagcggcta cgtcaagaag ttcggggccc agatctcctt cacgccggtc gccaccacgg
      541 tgaccacccc gcagcctggg ccgacggctc cctcaccctc agactggaac cagccgtagc
      601 cactacggcg gccgccgggc gcagcatctc acgatgcagc gcgcccggcg gttttccttt
      661 ttatatacct agcgaacctc gacgagctcg acctcgaaga ggagcgtcgc gttcggcggg
      721 atgacgcctc cggcgccccg ctcgccgtac gcgagcgcgg gcgggatcac gagctgccgc
      781 ttcccgccca ctttcatgcc cgccacgccc tggtcccatc cggggatcac ctcgcccgca
      841 ccgagggtga aggtgaaggg tcgaccatgg cggtcggagg cgtcgaagac ggtgccgtcg
      901 ggcagcatgc cgatgtaatt cacggtgacc tgcttcccgg cggccgcttc ggcgcccgtg
      961 ccgacaacga cgtcgcgcat cgcgagctcg gtcgggagcg gctggttggg atcgaggaag
     1021 gattgcatgg tggtggttgc cgactgttca agggcgaccg gctccgcagg cgcaaacggc
     1081 gtgaagaccg ccggtccgaa gtagaggaag ccgagtgcga tcacgacggc gagcccgacc
     1141 gcgatacctg tacccgtgac attcatagac taggtggtga ttgagtaagc gacagcgacg
     1201 atggcgatgt cccggccagt atatcacttg aattcccggg ccttgggccg gccgagctgc
     1261 tccctgaagg ccttacggat ctcttcgtcc gtgagctcct tcggttctgg gcgggtcatg
     1321 gtgacggtcg tctcgaacgg ggccgcatcg tatgccggct ccgcggagaa tggccttggc
     1381 tttgccacgg gcgccgtgac tgactccgac gagaagggcc tgggcttcgc agcaatgggc
     1441 gggaccgcct gcgcttccgg ggatgctgcg gccggtgccg gttcaggcac cggactccag
     1501 gcggcccgct tcccatcgaa gaaggtgccg ttcgggtacc cgagctcctt caccatgcgc
     1561 tcgcggacct tcttctcgtc ctcagggcga taccctttgg gatagtaggt agcggcatag
     1621 agaccgaata tccgctccca cgccttgtgc tgctggggcg tgatgctccg gtgcttgcgt
     1681 accttgggcg ccttcttctt tacctccttc tcgacctcct gagccgcctc ctgcgctggc
     1741 gcctctgcgg cggggttcgt cgtcgccccc gttccggccc attcccgaag ggactggatc
     1801 tgggctcccg caggccactg catgggatcg tacgggagcg tgatggactc cggggcatcg
     1861 gcggtggcgc gcgccacacc tgcatcttcc gcgttcttcg ccgcagccgc cgggccggtc
     1921 gatgccttta ccgtggatcg ctcctgatcg ctgccctgat tcgcgaagat tgccgcgaag
     1981 agaccagcgg cgagcccggc gcccccaagg cccgctcccc atgcggtcct cttacccggc
     2041 ttccaccagg gtcgtttctc acttatcgct gcgttgggtg caggcgccgc ggtatccgag
     2101 taccgcggat ctgccgggaa gggcccgaac ttcgcaagcc gcttcttatc ccaagagttc
     2161 cagcccatag cctgcctggt gaggacggag ttgaccatga ccgcgcgagg ttcctcggcc
     2221 ggagcagctt ctggcgccgg tgtcgcttct ggagcttgag cgatgggagt cgggacgggt
     2281 tccagcttcg gcttaggctc ctgcggcgct cgccccgcct gcctatgctg ctccatctcg
     2341 tccgagaact gtccccagac gcctccttcg tcatacgcga ggccgggttc cggctgcttc
     2401 gcttcgacac cctcgtcata ttcgaggcgc gcgatatgct cagcgagctg ctcctcatgc
     2461 tcggcatgcg tttcgggttt cggatcgggc tccggtccgt cctggcgctt tgaggcgcgt
     2521 tcgagcgctc cgagctgctc ttcaagcgca ggggccggct gcgccacgtc cttctccttc
     2581 ccgccgagac ggttgcggat cgctgcgatc ttttcctggt cgccatcgct gccgcgggct
     2641 cgatcggctc caacgacgtt tgcatacgcc ttcgcggact ccttggagag tttttcatgg
     2701 cgcttcagga gggcctcccg ccgcttcgat ccgggctcat cagtgatgcg gctgagattc
     2761 cgcacgattg catcgtgcgc atcgtacgcc cgcttccagt cctcggagat ctcctctttc
     2821 gagatcccgg ctgcctggac ttgatcgaca aagctctgcc agtcggcgcg ggcctgcgcg
     2881 atcagctcag cggcgcgctg ctgctccgct tcgggaacac gcgtaccctg cggaggtgat
     2941 tccgtctttg gctgtgcctt tttgggaccc tgttcagcgt tcgacataga gcttaggtaa
     3001 gatagcggct cctgatcatt gactccacgg taccacggtc ccggccgtac gtgaggtagg
     3061 acaactggcg aaggtcatcg atctgtcctg gattgcctgc ggaaggcggg agcgtcttga
     3121 agttgaaggg cttctgcggc accccgttgg cgagcagccg ggcgtacgcg ttgtagttct
     3181 cgatgttcac gaaatcgagc gcggagaaga ccggcgcgaa ctgcttcgcg aagtattccc
     3241 cgtcctcttc gcccacccga aagaccgcca tgctgcccac gttgccgaag actgcgttcc
     3301 ggatcttctc gtccacctgc tggaggaact ggtgcgcgat cgtgagcgag agcttgtact
     3361 tgcgcgcctc cgagaggatg cccgggatgg aatcggtggt gatgttctgg aactcgtcga
     3421 tgtacaggta gaagggcggg aagtcgccgc ggggattatc ggcgcgggac agggccgcca
     3481 tgaacaactt cccgacgagg atgagcccga gcaggttcgc gttgcgctcc cctaaccgcc
     3541 ccttcgagag gttcaccagc aggatcttct tgttgtcgat cacctcccgg aacttgaagg
     3601 aggattcctg ctgcccgatg atcgggcgca tgaagtcgtt tgcggtgaag tcatcgaatt
     3661 tgttcgtgat gtagggcacg atgttcgcca gggacgcctc gccctccgcc tgcgtcgcga
     3721 tctccgtcca gaactggttc acgaccggat tcatcgactt cgcgagcttc aggttccgga
     3781 aggacgcgtt gctgagcacc cgcccgatgt ccatcaaggt ggaacccgag gagggatcct
     3841 ccatgacgag cagggtcgcg ttccggaagt actgctcgaa cgcggggccc atggactccg
     3901 gcacgtcccc gtacaggcgc cggaagatgg cgaggagctc gttcacgatg aaggtcttct
     3961 gctcggggtg cgcgggatcg tactccagca ggttgaggcc cagcggacga tcgaggtacg
     4021 cgggatcgaa gtagatgacg tcgtcatagc gctcgggcgg gaccgaggcg agcacatcga
     4081 ggatgtcgtt gccgtgcggg tcgatgaagc agacgccgtt gcccgccttg atgtcctgct
     4141 cgatcagggt cttcataaag gacgtcttgc cggtgcccgt ctgcccgatg acgtagaggt
     4201 gccgcagccg gtcctccggc gagagccgga cctcggtggt cgcgccccgg aaggcattgg
     4261 tcccgaagag gatgcccgtc ttcgaaagct ccaggggcgc cgccgccatg gtgaagcggg
     4321 cctgggtgag gtgcggcgcc gattcgatgc ccttcg
//
LOCUS       DASPKG010000086         6095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_524710, whole genome shotgun sequence.
ACCESSION   DASPKG010000086 DASPKG010000000
VERSION     DASPKG010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524710"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1805)
                     /locus_tag="VEA36_03445"
     CDS             complement(66..1805)
                     /locus_tag="VEA36_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29391.1"
                     /translation="MGRRALVLWGEVPTRPPAWWREFDTVFARRTHTEVLEQLGVAYE
                     DLDAILDPGSIFEATDMVRALARITLPDGSRLAKSVLYKGYELWWIHYDDLHLKFCLP
                     FTQYEPLLKALTAYEEVHSYNPPSSTLFGYYLEAHGVRYEAIDDPSPDRLPFGMVLQI
                     ILSLPFLIALMIRRPGLMLWTSDRFDPPRDHDFRTRFLYEELHNRRIPFVEFIRSQET
                     WKTMLEHAKVRRRPVIYSHAITTALRLLAGVLVKKDDHRVRALVTQQGGTPEETFRFL
                     LATHYLRNVTGDIWAIRAMQLVLWAIGIRAAYVPAALSRNFHEVLAAKRSRIPIIGIL
                     HGVSSKSYNVYDFMPEYDGTLSLSVDRYGLWSPWWREYYLENSKVYKPEQLAVSGPMR
                     PVERGSLPEKKAERTPGEPIKVLFIAEQLGIPKEILPYLTALLDAPDLSFHMKVRSYK
                     DGFEEWLAANRPDLIERMDQERIFRGGMNEAIAACDVVVGSHSTAVLESLLQLKPCIF
                     YDTKKWGDYFDLRSFRSDHKLIGDTPEEFLGLVRQATDIPEATLKELQYRFFGDPYEN
                     GSAWVVDELAKRL"
     gene            complement(1809..3602)
                     /locus_tag="VEA36_03450"
     CDS             complement(1809..3602)
                     /locus_tag="VEA36_03450"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYF29392.1"
                     /translation="MPKTSFVIRTFNEEKHLGNLLGAIAAQDYDDYEVVIVDSGSSDT
                     TLEIARKFKARIVQIESKDFTFGHSLNVGVEAATGEYIICASAHVLPLGKDWIRSMIE
                     PFADEKVAMVYGRQVGSPTSKYSEKRDFERLFGESEFDSRVFLEYANNANSAIRKALW
                     QKHQFDPTLPGLEDLEWAKHMTGEGYVVRYAPRAAVYHIHEEKWHQVLNRYRREAVAA
                     QHMQLPHPPQARIEFPWLVKMLLGDLLSALPVLTPALFEEILRFRYYQWKGTRLGWYG
                     NPDLKVQAERYKLYYAPLTYKVLITAPYFQLALDDYRDFFATHGIELIVPTVNERMEE
                     ADLLPLMKDVDGILCGDDRITARVLDQAPKLKAIVKWGTGIDSIDKEAAKARGITVRN
                     TPNAFTEPVADTVMSYILCFARNTIGLDRLMHQGGWEKQLNPALHECTLGIIGMGNIG
                     QALARRAQAFGMRVIATDIVPKSFPNMVPLDELLAEADFVSVNCDLNPTSEHLMNAER
                     FAKMKPTAYFLNAARGPIMDETALIEALQNKTIAGAGLDVFEHEPLPVDSPLRAMDNV
                     ILSPHNSNAGSLAWKRVHENSLRSLFEELSK"
     gene            complement(3604..4392)
                     /locus_tag="VEA36_03455"
     CDS             complement(3604..4392)
                     /locus_tag="VEA36_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HYF29393.1"
                     /translation="MDHQPIAIGIIPARMGSTRFPGKPLAPILGIPMVGHVYHRAQMS
                     TALAEVYIATCDQEIGDYADSIGAKWVMTKDTHKCAPDRAAEAMLKIEAETGTKTDIV
                     VMIQGDEPMITPEMIDMAVAPFSADASIEVLNLMAPITSEEEHNDPNCPKVVVDRNNF
                     ALYFSREPIPSKKKWNGKDAFPKYKQVCIMPFKRDFLLTFNALPYTPLELVESIDMNR
                     VLEHGHRVKMVLEEYETYCVDTPQDLVRVEAHMQGDELMKTYAA"
     gene            complement(4518..4979)
                     /locus_tag="VEA36_03460"
     CDS             complement(4518..4979)
                     /locus_tag="VEA36_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29394.1"
                     /translation="MRTPRTNTHVYGEHAFWLKDSEVKMARDGTLSVIVRGGDRSTPE
                     KAPKQFLRTNEPVPCYMIAVPGDQDKGISAQFEADGGTTVEVVERIVKPLGEFTDDEL
                     VFEGGRAVPENAAGLKRYLEEELAPGKTFGPDAVITVYHVRRLPNEEPATD"
     gene            complement(5043..>6095)
                     /locus_tag="VEA36_03465"
     CDS             complement(5043..>6095)
                     /locus_tag="VEA36_03465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYF29395.1"
                     /translation="GLTVHTLYSSYPVWRRAWVSLRNPQVLPEVRRILTEFRPEIVHA
                     HNVHTHLSYASLKVAHELGARVVLTAHDAMTVEYGKLVPRGGDLRVSPLRQFLTQRHR
                     YNPLRNATIKRYLKYVDRIVAVSHALKRALEANGITNVEVIYNGIRVEDWEVAESAVA
                     AFRERFELANKKTILFGGRLSKLKGAETAVRVLAEVAKTVPNATLLIMGRQGPYAKEI
                     KALARTLGVVDRLIFTGWISGDELRAAYHASDVVAVLSIYLDPFPTITLEAMACGKPV
                     IGTSLGGTSEAIEDGVTGYVVDPCNTAEAGKKTAELLTDDARSAAFGQQGRRRVSQVF
                     SLESAVDAYSAICTNI"
BASE COUNT         1078 a         1820 c         1964 g         1233 t
ORIGIN      
        1 ggccgatacg tgagccggct cgtgacggtg gccgcaaatg cggtggcgct ccactacctt
       61 acccgctaga gccgcttcgc gagttcgtcc actacccagg cgctcccgtt ctcgtacggg
      121 tcgccgaaga agcggtactg caattccttg agggtcgctt ccgggatgtc ggtcgcctgc
      181 cggacgagcc cgaggaattc ctcgggcgta tcgccgatga gtttatgatc cgaacggaag
      241 gagcggagat cgaagtagtc gccccacttc ttggtgtcgt agaagatgca gggcttgagt
      301 tgcagcaggg actcgagcac cgcggtgctg tgcgacccca ccaccacgtc gcaggccgcg
      361 atcgcctcat tcatgccgcc ccggaagatg cgctcctgat ccatgcgctc gatgaggtcc
      421 ggacggttgg ccgcgagcca ctcctcgaac ccgtccttgt acgagcgcac cttcatatgg
      481 aaggagaggt cgggagcatc cagcagcgcc gtcaggtacg ggaggatctc cttcgggatg
      541 ccgagctgct cggcgatgaa gagcaccttg atgggttcgc ccggtgtgcg ctccgccttc
      601 ttctccggaa ggctcccccg ctcaacggga cgcatgggac ctgagaccgc gagctgctcg
      661 ggcttgtata ccttgctgtt ctcgaggtag tactcccgcc accacggact ccagagcccg
      721 tagcggtcca ccgagagcga aagggtgccg tcgtactccg gcatgaagtc gtagacgtta
      781 taggacttcg aggagacgcc gtgcaggatg ccgatgatgg gaattcgcga gcgctttgcg
      841 gcgagtacct cgtggaaatt gcggctgagc gcagccggga cgtaggctgc gcggatgccg
      901 atcgcccaga gcacgagctg catcgcgcga atcgcccaga tgtcgcccgt cacgttccgg
      961 agataatggg tcgcaagcag gaagcggaac gtctcttcgg gagtacctcc ctgctgcgta
     1021 acgagcgcgc gtacccgatg gtcgtccttc ttcactagca ctcctgcgag gagccggagc
     1081 gcggtcgtga tcgcatgcga gtagatgacc ggacgtcggc gcaccttcgc atgctcgagc
     1141 atggtcttcc aggtctcctg gctgcggatg aattcgacga acgggatcct gcgattatgc
     1201 agctcttcgt agaggaagcg ggtacggaag tcatggtcgc gcggcggatc gaaccgatcg
     1261 ctcgtccaga gcatgaggcc cgggcgccgg atcatgagtg cgatgaggaa tggtagcgac
     1321 agaatgatct gcagcaccat accaaacggc aaccgatcgg gagacgggtc gtcgatggct
     1381 tcgtagcgca ctccatgcgc ctcaaggtaa tacccgaaga gtgtgctcga gggcgggtta
     1441 tagctatgta cctcctcgta ggccgtaagc gctttcagca ggggctcgta ctgcgtgaac
     1501 gggaggcaga acttgagatg gaggtcgtcg tagtgtatcc accagagctc gtagccttta
     1561 tagaggacgc ttttcgcgag ccggctgccg tcgggaaggg tgatgcgcgc gagcgcgcgg
     1621 accatatcgg tcgcttcgaa gatgcttccc ggatcgagga tggcatcgag gtcctcgtac
     1681 gctacgccga gctgctcgag gacttccgta tgcgtacgcc gcgcgaagac ggtatcgaac
     1741 tcgcgccacc atgcaggcgg gcgcgtcggt acctctcccc aaaggacgag cgcgcggcgt
     1801 cccatgcgtt atttcgagag ctcctcgaag aggctccgga ggctgttctc gtgcacgcgc
     1861 ttccaggcga gcgatcccgc gttcgagtta tgcggggaga ggatgacatt gtccatggcg
     1921 cgcagcgggc tatcgaccgg gagcggctcg tgctcgaata cgtcgaggcc cgcgcccgcg
     1981 atggtcttgt tctgcagggc ctcgatgagc gcggtctcgt ccatgatcgg gccgcgcgcg
     2041 gcgttcaaga agtaggcggt aggtttcatc ttcgcaaagc gctcggcgtt catgaggtgc
     2101 tcgctcgtcg ggttgaggtc gcagttgacg ctcacgaagt cggcctcggc caacagctcg
     2161 tcgagcggga ccatattcgg gaaggacttc ggcacgatgt cggttgcgat gacgcgcatg
     2221 ccgaacgcct gcgcccttcg tgcaagcgcc tgcccgatgt tccccatgcc gataatgccg
     2281 agcgtgcact cgtggagcgc cgggttgagc tgcttctccc agccgccctg gtgcatgagg
     2341 cggtcgagcc cgatggtgtt gcgcgcaaag cagaggatgt agctcatcac ggtgtccgcg
     2401 acgggctcgg tgaaggcgtt cggcgtattg cgcaccgtga tgccgcgggc cttcgccgcc
     2461 tccttatcga tggagtcgat gccggtgccc cacttgacga tggctttcag tttcggcgcc
     2521 tggtcgagca cccgtgccgt gatccggtca tccccgcaca gaatgccgtc gacatccttc
     2581 atgagcggga ggaggtccgc ttcctccatg cgctcgttga cggtcgggac gatgagctcg
     2641 atgccgtgcg tcgcgaagaa gtcgcggtaa tcatcgagcg ctaattggaa gtacggcgcg
     2701 gtgatgagga ccttataggt cagcggcgcg tagtacagct tgtagcgctc cgcctgcacc
     2761 ttgaggtccg gattgccgta ccagccgagc cgcgtcccct tccactggta atagcggaac
     2821 cggaggatct cctcgaaaag cgcgggggtg agtacgggaa gcgcgctcag gaggtcgccg
     2881 aggagcatct tgacgagcca ggggaattcg atgcgcgcct ggggcggatg cggcagctgc
     2941 atgtgctgcg ccgcaacggc ctcgcggcgg tagcggttca ggacctggtg ccacttctcc
     3001 tcgtggatgt ggtagacggc agcgcgcggc gcgtagcgca ccacgtaccc ctccccggtc
     3061 atgtgcttgg cccattcgag atcctcaagg ccgggcaggg tcgggtcgaa ctggtgcttc
     3121 tgccagagcg ccttgcggat cgcggagttg gcgttgttcg cgtattcgag gaagacccgg
     3181 gaatcgaact cggactcgcc gaagaggcgc tcgaaatccc gcttctcgga atacttggag
     3241 gtagggctgc cgacctggcg gccgtacacc atcgcaacct tctcgtccgc aaacggctcg
     3301 atcatcgaac ggatccagtc cttgccaagt ggcagcacgt gcgcggaggc gcagatgatg
     3361 tactcgcccg ttgccgcctc cacgccgacg ttgagcgaat ggccgaaggt gaagtccttg
     3421 ctttcgatct gtacgatccg ggccttgaac tttcgtgcga tctcgagcgt tgtatcgctc
     3481 gagcccgagt ccacgatcac cacctcgtag tcatcgtagt cctgcgcggc gatggcaccg
     3541 agcaggttcc cgagatgctt ctcctcgttg aaggtgcgga tgacgaaact ggtcttgggc
     3601 atattaggca gcgtaggtct tcatgagctc gtcgccctgc atatgcgcct cgacgcggac
     3661 gaggtcctgg ggcgtatcga cgcagtacgt ctcgtattcc tcgagtacca ttttcacgcg
     3721 atgcccgtgc tcgagcacgc ggttcatgtc gattgattcc acgagctcga gcggagtgta
     3781 cggcagcgca ttgaaggtca ggaggaaatc gcgcttgaac ggcatgatgc agacctgctt
     3841 gtacttcggg aaggcgtcct tgccgttcca tttcttcttg gaggggatcg gctcgcggga
     3901 gaagtagagc gcgaagttgt tgcggtccac caccaccttc gggcagttcg ggtcgttatg
     3961 ctcctcctcg ctcgtgatcg gtgccatgag gttgagcacc tcgatcgagg catcggccga
     4021 aaacggcgcg accgccatat cgatcatctc gggggtgatc attggctcat cgccctggat
     4081 cattacgacg atgtccgttt tcgttcccgt ctccgcttct atcttcagca tcgcctcggc
     4141 agcacggtcg ggggcgcact tatgtgtatc cttggtcatc acccatttgg cgccgatgga
     4201 gtcggcataa tcgccgatct cctggtcgca ggtggcgatg tagacctcgg caagcgcagt
     4261 gctcatctgc gcccggtggt agacgtgccc caccataggg ataccaagaa tgggcgcaag
     4321 cggcttcccg gggaaccggg tcgagcccat gcgggcgggg atgatgccga tagcaatcgg
     4381 ttgatggtcc atatagtact gaaacgcctc gtggtaataa aaagtagcgc attttagggc
     4441 atcctccaag gcccggttgc attaaacagg gcccgccttg cagcgggccc tgtggcactg
     4501 gggacggtcg cgccgttcta gtcggtggct ggctcctcgt tcggcaggcg ccggacgtgg
     4561 tagacggtga tgacggcgtc cgggccgaag gtcttgccgg gcgcgagctc ctcttcgagg
     4621 tagcgcttca ggccggccgc gttctcgggc actgccctgc cgccctcgaa cacgagctcg
     4681 tcgtcggtga actcgccgag gggcttcacg atccgctcga cgacttcgac ggtggtgccg
     4741 ccgtcggcct cgaactgggc gctgatgccc ttgtcctggt cgccgggcac cgcgatcatg
     4801 tagcagggca ccggctcgtt cgtgcggagg aactgcttcg gcgccttctc gggcgtcgag
     4861 cggtcgccgc cgcggacgat cacgctgagc gtgccgtccc tggccatctt cacctccgag
     4921 tccttgagcc agaaggcgtg ctcgccgtag acgtgggtat tcgtcctggg tgtgcgcatc
     4981 ggtctcccct attgcgcggg ttgaacttac cggaaatagt atgacagatg acgaacctta
     5041 tgtcaaatat tggtacagat cgcactataa gcgtcgacgg ctgattccag gctgaatacc
     5101 tgggataccc gccgtcttcc ctgctggccg aatgcggcgc ttcgagcgtc gtccgtaaga
     5161 agctcggcag ttttcttccc tgcttctgcc gtattgcatg gatcgaccac gtatcccgtc
     5221 accccgtcct cgatcgcttc agacgtaccg ccaagcgacg ttccgatgac cggcttaccg
     5281 caggccattg cttcgagtgt tatggtcggg aatggatcga ggtatatgga gaggacggca
     5341 accacatcgc ttgcgtggta tgcagcacgc agctcgtcgc ctgagatcca tccagtgaat
     5401 ataagacgat ctacgacgcc gagtgtgcgc gcgagcgcct tgatctcctt cgcgtaggga
     5461 ccctgcctgc ccatgataag gagcgtcgca ttcggaacgg tcttcgcgac ctcggcgagc
     5521 acccggacgg ccgtttcggc gcccttcaac ttcgagagcc gcccgccgaa gaggatggtt
     5581 ttcttattcg cgagttcgaa gcgctcccgg aacgctgcaa cagcggattc cgccacttcc
     5641 caatcctcca ctctgatccc gttatagatc acctctacgt tcgtgatccc attggcttca
     5701 agcgcacgct tgagtgcatg actcaccgcg acgatgcgat cgacgtactt gagataccgt
     5761 ttgatggtcg cattgcgcag cggattgtac cggtggcgct gggtaaggaa ttgccgcagg
     5821 ggcgacacgc gaagatcccc tccgcgcggc acgagctttc cgtactcgac cgtcatcgcg
     5881 tcatgcgcag tgagcacgac ccgggcaccg agctcgtgcg ccaccttgag tgatgcatac
     5941 gagagatgcg tgtggacgtt gtgcgcatgc acgatctccg gacgaaactc ggtgaggatt
     6001 cgccggacct cgggaaggac ctgcgggttc cggaggctca cccacgcgcg tcgccatacg
     6061 ggatacgagg aatagagcgt atgaacggtg agacc
//
LOCUS       DASPKG010000087        20218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_928865, whole genome shotgun sequence.
ACCESSION   DASPKG010000087 DASPKG010000000
VERSION     DASPKG010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 20218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20218)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20218
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928865"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VEA36_03470"
     CDS             <1..820
                     /locus_tag="VEA36_03470"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HYF29396.1"
                     /translation="ERGKASSPDRILPTVDVAVVALHGAYGQDGEVQKLLERFGVAYT
                     GADPWACYLSAHKLMAKKHVEEVGLLTPRYHFIDQVTEIDRIVHEAIRTFHQPVVVKP
                     VDSGSSVGVSIVGGYAPVHLAVTNLFLAGTKGVLIEELVKGTEATVGIVEGLRGEELY
                     ALPPVEIVPSAAFFSYEAKYSGQSREICPGRFDRRVSEALMDAARRAHEALGLRHYSR
                     SDFIVSPKGIYYLETNNAAAVGMTGESLFPKSLAAVGVGLPEFLEHLIERAKRH"
     gene            822..1157
                     /locus_tag="VEA36_03475"
     CDS             822..1157
                     /locus_tag="VEA36_03475"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HYF29397.1"
                     /translation="MEELSKKKCKPCEDDGFPALTKEQAGDLMQHIPEWTLSERGTSI
                     TRQFPFKDFKEALAFANKVGELADEEWHHPDLIVAWGRVEVLLTTHSVKGLSENDFIL
                     AAKIDLLEK"
     gene            1205..1277
                     /locus_tag="VEA36_03480"
     tRNA            1205..1277
                     /locus_tag="VEA36_03480"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1238..1240,aa:Ala,seq:tgc)
     gene            complement(1369..1527)
                     /locus_tag="VEA36_03485"
     CDS             complement(1369..1527)
                     /locus_tag="VEA36_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29398.1"
                     /translation="MNRPFFTPAEPVVSPEALLLARLYVAADVLDEAIAAYAEDLGLG
                     LPLTEDFD"
     gene            1662..1928
                     /locus_tag="VEA36_03490"
     CDS             1662..1928
                     /locus_tag="VEA36_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29399.1"
                     /translation="MDNLHPQVAKRVGITTLKDGMGIEDRGILGVNFYSSFTSANIHP
                     SIEDRMLTVGVRKSALGELYLILKQLIEESPREYGFEIKVEVVE"
     gene            1951..2520
                     /locus_tag="VEA36_03495"
     CDS             1951..2520
                     /locus_tag="VEA36_03495"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYF29400.1"
                     /translation="MSTIYILHGCPSDEEKAMNPETRTYDKHWIPWLRAKLESEGRSV
                     KTPLMPNPWAPDYDAFKTEFEKNAIGEKDVLVGHSCGGAFLVRWLGDTKQKMAKLILV
                     APWKIPALGDELRRKYYGYEIDRTIPDRVGEIVIFTSDDEEEDGKRSAHIFHEALGGD
                     LVELPKHGHFTMGDMGTVEFPELYQTVIT"
     gene            complement(2752..3177)
                     /locus_tag="VEA36_03500"
     CDS             complement(2752..3177)
                     /locus_tag="VEA36_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29401.1"
                     /translation="MGFLERLGLGVPTPEDRARLQQSIDANHEAEANLAQAMRHRGVA
                     VGKLDLLPEYRERTRLEIALQQAETKLNGARDRVAHLDELRRLRTQLAAMRGQTVPAD
                     GQEAAAARAELATEQAAYDSVKTELDRLNVEITQKESRL"
     gene            3275..3646
                     /locus_tag="VEA36_03505"
     CDS             3275..3646
                     /locus_tag="VEA36_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HYF29402.1"
                     /translation="MATYVDGFVLTVPKKQLAAYKKIATEARDIWMKYGALDYKECVG
                     EDLKPKQVKLTFPKMIAAKPTEVVFFSFIVYKSKADRNKINKQVMAHFEKKYGKDMKM
                     PVDMRRFAYGGFTSLVEAKKK"
     gene            3752..4300
                     /locus_tag="VEA36_03510"
     CDS             3752..4300
                     /locus_tag="VEA36_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29403.1"
                     /translation="MILDIVTGDITTPENPSDIIIGMNSQLSDVTGIGLPFVKDVIPM
                     RQIQLGSVITFEFDIHRRLHMLICHHLGKGGWAYADRHVRYGLDHLEYLDREQQAGGS
                     DRTYSIVKIGTGRVGVRDGADAPALHRAMADSFLPLTLFLYNRETVEAVTRESRAPLV
                     PISTWSPDHGRQRIHAPAVAMT"
     gene            complement(4366..4439)
                     /locus_tag="VEA36_03515"
     tRNA            complement(4366..4439)
                     /locus_tag="VEA36_03515"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4405..4407),aa:Pro,seq:cgg)
     gene            complement(4441..4514)
                     /locus_tag="VEA36_03520"
     tRNA            complement(4441..4514)
                     /locus_tag="VEA36_03520"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4478..4480),aa:Val,seq:cac)
     gene            4580..4987
                     /locus_tag="VEA36_03525"
     CDS             4580..4987
                     /locus_tag="VEA36_03525"
                     /inference="COORDINATES: protein motif:HMM:NF021462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HYF29404.1"
                     /translation="MKNTLVLALLIVPTLIILDLLWIGLIAQGIYQDQIGYLLATEVT
                     WWAALLFYVIYGVALAHFVVLPAIKLGDMRRALYNGLLFGLAAFATYDLTNLATVRDW
                     PVALTFIDMVYGTIVAGVTSATAYVLGKKLVRG"
     gene            5004..5074
                     /locus_tag="VEA36_03530"
     tRNA            5004..5074
                     /locus_tag="VEA36_03530"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5036..5038,aa:Pro,seq:ggg)
     gene            complement(5249..6325)
                     /gene="mltG"
                     /locus_tag="VEA36_03535"
     CDS             complement(5249..6325)
                     /gene="mltG"
                     /locus_tag="VEA36_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HYF29405.1"
                     /translation="MPGTPPKSLMPHPAASSKKAPQGVFKKLAIQKKSIGPVAGATLL
                     LALGFFALPSVLAVDLHTAESPKPDFPVTVDPIARVIIEDPEVEALLAEDNPELAAAA
                     GALDMAYTWLAATIANSGLYRSLASAVGADTAFVTVLPGYREEEVAAAFGKTLGWSAA
                     ERREFLKQLHSTPPTLTEGELVPGTYAVPTNAEREDVQALLNERFSREILSRYSTSTA
                     EVVPLDDALTIASMLERETRDPAEMRMISGIIWNRLFTGMNLQIDATLQYAKATNTDT
                     KSWWPKVVPNDKYIKSPYNTYRHKGLPPGPIANPSTEAVIAALNPKKTDCLFYFHDKY
                     GGFHCAPTYEEHVRLLKEKYGRGR"
     gene            6419..6928
                     /locus_tag="VEA36_03540"
     CDS             6419..6928
                     /locus_tag="VEA36_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29406.1"
                     /translation="MQRITPRQYTGVDRIVSAIGSVPSLVIHSLAFAGFFLIAVFGIA
                     SWEIVLLVLTTIVSLEAIYLAIFIQITVNRHTQSLREVEEDIDEIQEDVEELGEDVED
                     IQEDIEEIQEDFEEMSDEEKGEHLIREEKKRVKKVADEAELYEKLTKDVERVLRDLET
                     LKRASGDKR"
     gene            6968..9367
                     /locus_tag="VEA36_03545"
     CDS             6968..9367
                     /locus_tag="VEA36_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HYF29407.1"
                     /translation="MPRLLPALLIGFIVLLVPFKSDAILLTSDLSIGSRGAEVTQLQL
                     ILFQFGYSTAAPNGVYSETTADGVRALQKKYGLAVTGTLDARTRALLNPFISRNPTSG
                     ATRPAAASSGIASGGTAALGLGSSGPAVLEVQQKLAALGYFKETPTGYFGPITRAAVI
                     TFQAAKGLEQVGQVGPLTRAALAGAGPVVASSGGTSSSSIGVTPAATQTKTTGERPPA
                     LTFRIDRKFALVGDYIYLNWSAKRADRCTASGGWSGEKGTRGTDSFAVAGDTTYTLTC
                     TGSGGSVTKSVWVSTTLVARKAEEPEEIAETPVVTVPTTPTTPTTPATPTTPTTPKPT
                     PILTTLPGPSKSYQVYAGCEAPAKSYARVIYVDPVNGTDAGDGSAAAPLKTASQALAS
                     KKIKPGDHVVLLPGNHGRLYADGVYGSAEFKDAANWTWLDFQEGAYISNLDFRNGMSR
                     WLITNAEASNQFSTSARTMIATSGGIHFVFADGELYSIKNASSWSASDWLNKAASGIS
                     TRNTRCVSILDNDIRNVAGGITLQIDANVSDNSIKGLVARNTIFGNQADGLRPLGSDI
                     TMRDNRVIESMLGSEHGDGNHDDGLQIFPIGSHVFNNIRIEGNWIQESTASGRPLIGG
                     QQGIGHFGGPLKNSVIRNNVILSSVWHGISTYDPENVIIEHNTVLNTSGNKRATWIAA
                     WKGGKAENPTSGVIVRNNIAHSFILGNGVASSSNIVIAPDAPAFVQFDHVNGRFDLRP
                     KPGGPLDGKNAGASGVSSGLGSQAASGNPYAQTAAAAQALPTLEQLYLQLQNLLNTLR
                     N"
     gene            9420..11810
                     /locus_tag="VEA36_03550"
     CDS             9420..11810
                     /locus_tag="VEA36_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HYF29408.1"
                     /translation="MNLTRPFFLFLVAAALFMGAAHHAHAALLVSDLSIGSRGAEVTQ
                     LQLILFQFGYSTAAPNGVYSEATADGVRALQKKYGLPQTGIVDAKVRGFLNPFIARNP
                     TSGSTRPASPTAGSVGGGTAALAFGARGAAVLELQQKLAALGYFKETPTGYFGPITRA
                     AVAAFQAARGLETVGYVGPRTRAALAEVPTGTATAAGSGTSPRVSGTTTTGERPPALT
                     FRIDRKFAIVGDYVYLSWSAKRADTCTASGAWSGDKGTKGSDALTVAGDATYTLTCTG
                     SGGSVTKSVWVSTTLVARKAEEPEDVTSTPVAVTPPSSTPGSSAPPSPTPTPTPAPVP
                     TPLPGPSKSYQVYPGCEAPARSYARVVYLDPRNGSEAGDGSAAAPLKTLATALAAKKL
                     RAGDHVILMPGEHGAFVGDTSFSQKGLFFRDAPNWTWFDFQEGATISKSDLSGFSRIL
                     ITNPEVTPGGILVTGGSQVVVADGEFYLARNTADWTAEKWLAQGKGNTLMSRNTRCVS
                     FIDNELRNIPFGISISVDGLPTSDNSARALVARNTISRFRGDGIRPNGSDVIVRDNRI
                     VDSMLGREHGDGNHDDGIQLFALNGHRFKNVRIENNWIQETTSATRPYIGTLQGIGHF
                     DGVIEDVVVRNNVVLIGAYHGIMLNSVGGTVENNTVAYVGTEGRNTWIRMGASSQNIT
                     FRNNVARFFEYPTSAGRIQIRAPQQDAIGNNITVKDNESAAFMKFDPANGAFDLRPKP
                     GGPLDGKGVGAAGLTSGLGSQAVLKDPSAQTAGAGNALPSLADLYEQLMNLLKTIR"
     gene            11822..14233
                     /locus_tag="VEA36_03555"
     CDS             11822..14233
                     /locus_tag="VEA36_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HYF29409.1"
                     /translation="MHTRLIALLIAACMVMPVPAHAVTLTSDLSIGSRGAEVTQLQLI
                     LFQFGYSTAAPNGVYSETTADGVRALQKKYGLAVTGTVGAKTRALLNPFIARNPTSGS
                     TRPAPSASGGGSPLMLGSSGPAVLEVQQKLAALGYFKETPTGYFGPITRAAVIAFQAA
                     RGLEQVGSVGPLTRAALAGAGPASATSGSVSSSSTGVSSASTRTATTGERPPALTFRI
                     DRKFALVGDYVYLSWSAKRADTCTASGGWTGNKGVKGSESFSVPGDTTYTLTCTGSGG
                     SITKSVWVSTTLVARKVEEPEEVTETPVVVAPVTPATPATPTTPSNPALPAVPATPAV
                     PKPPIPTTLPGPSKNYQVYQGCEAPAKSYARVVYIDPQHGLDTGDGSSAAPLRTLQAA
                     FSAKKLRAGDHVVLMPGNHGEYTGDGRYNQSGLFTTPNWIWLDFQEGAVMGRLELRNI
                     SRILITNPEFNPSAKVGQMLLVSGGTDLVVADGEFYSIKNSGNWSASDWLGKTSSGIF
                     VRNHRCASVLDNKLRNVAFGISISTDGFPTSDNAARALVARNEIIGFQGDGIRPNASD
                     VSVRDNRIIESMLGAADGDANHDDGIQIFALNGHEFSNIRIENNWIQETTSGSRPMIG
                     SLQGIGHFDGIIKDSVVRNNVVLVSAYWGIALSGPKDSIIEKNTAVNSASFSRGVRIA
                     ANATKATWGSVPPQNTIVRDNVAQVFFTTTGTVTASNNVVVGQDMSNVFVTFDKANGT
                     YDLRPKPGGPLDGKGAGASGISSSLGSQAVLSDSYAQTAGAANALPSLADLYQQLVDL
                     LKTLK"
     gene            14283..15053
                     /locus_tag="VEA36_03560"
     CDS             14283..15053
                     /locus_tag="VEA36_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HYF29410.1"
                     /translation="MIRSNSSVTSLALPRYLTVAAALVFLFAPSFAQAASLSPSTLTK
                     LVNAERTAAGLTALSTNAALTRAAQAKANDMAAKGYFAHTTPDGKSFSHWVVNAGYAY
                     KSAGENLAQGFSTESGTVGAWMNSPGHRTNIMASKFSETGIAVASGTYNGKSVIFVVQ
                     MFGAPSGSAGAVAAKPAPAPKVAAQAVPKARVLGAIEKPTALPNELEAKDASPEKTPV
                     SRTEMVAATAEADAQGRLLTLLMELRDLLLAAAGMVRA"
     gene            15097..15960
                     /gene="atpB"
                     /locus_tag="VEA36_03565"
     CDS             15097..15960
                     /gene="atpB"
                     /locus_tag="VEA36_03565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HYF29411.1"
                     /translation="MGGVLTQAGSFLHMVLGGGIARAAEDASHAVEGTEHSSAIHVVL
                     KAEEVFNLFGFPITNSLLLTWVTMAVLIGFAFYFRSRLALIPGKLQAGVEWAFEAVLN
                     YMEETLESRELARRFFPLIATIFLFILVANELAFLPGVGTSLGVDNGHGGLTPLFRAP
                     AADLNTTLALAIISFLTIEITGVLTLGLLKYGGKFVNFKSPVDFAVGLIELVSNLGRL
                     ISFSFRLFGNIFAGEVMILVAAFFLPYLLPVPLMAFEVFVGFIQAVVFAMLTLFFIKL
                     AIMEPHGETAH"
     gene            16015..16245
                     /locus_tag="VEA36_03570"
     CDS             16015..16245
                     /locus_tag="VEA36_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HYF29412.1"
                     /translation="MDVEAATQLGMALAAGLGVIGPGIGIGLIGGGAMQAIGRNPEAS
                     GKIVSNMILAIVFTEALGILALVVAFIIKFVG"
     gene            16252..16692
                     /locus_tag="VEA36_03575"
     CDS             16252..16692
                     /locus_tag="VEA36_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HYF29413.1"
                     /translation="MDALFAAFGIDWRLLLVQAVNFGVLFVALTYLLYKPTMKTLDER
                     RAKVAKGVEDAQAAEAKLAEADEVALGVRRGAEQEAEGIVASARESASAEKARIVKEA
                     ELRAAAVAADADARAKEAAAKALRESEKEIARLTILAAEKAMRT"
     gene            16694..17062
                     /locus_tag="VEA36_03580"
     CDS             16694..17062
                     /locus_tag="VEA36_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29414.1"
                     /translation="MEKVYAEALERSVLKGGDEGQLFAGLMRHLKAVGRLKLLPGILR
                     ELRARQSRLAKLAPSVEVASTAEKAVALKEAKAAGIEAADAVVNDALISGWRGRSGAQ
                     LVDRSGKQALIEIYRNIVTK"
     gene            17065..18612
                     /gene="atpA"
                     /locus_tag="VEA36_03585"
     CDS             17065..18612
                     /gene="atpA"
                     /locus_tag="VEA36_03585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HYF29415.1"
                     /translation="MESIIKRIESEIASYVPKGGTEETGIVRATGDGIAEIDGLDNAI
                     MTEMVLFDPTFDRTLEEALKDAAPIYGLVLNLEEDGVRAVILGDASKVFEGMLVRRVG
                     RLLSIPVGDSLVGRVVNPLGEPVDGKGPTGVKDIRPIEREAYGVIDRAPVNTPLHSGI
                     KAIDAMTPIGRGQRQLIIGDRGTGKTTIAIDTILNQKSEDTATRPICVYVAIGQKESK
                     TAKIVAQLAEAGAMDYTIVVTAPASSPAAFQFLAPFAGATIAEHFMEQGKDALVIYDD
                     LSKQAVAYRQMSLLLRRPPGREAYPGDIFYLHSRLLERAAKLSKEKGGGSITALPIIE
                     TQENDVSAYIPTNVISITDGQIFLETDLFNKGLRPAMNVGISVSRVGSAAQTKAMKKV
                     SGKIKLELAQFRELEAFMQFSSDLDADTKKRIDAGQRMTEMLKQPNGKPLPFEMQAAV
                     IFAATNGYFDTFTPSETAAAEERLQDFLRRGGTGVLEKIRTSREIAEETEKELRELLD
                     SFVERST"
     gene            18617..19141
                     /gene="yjjX"
                     /locus_tag="VEA36_03590"
     CDS             18617..19141
                     /gene="yjjX"
                     /locus_tag="VEA36_03590"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011250698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0046128 - purine ribonucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HYF29416.1"
                     /translation="MKIVVGSRNPAKVEAVREILADYPRFKDAEIVGIEVPSGVPDQP
                     TSFEEVTGGAVNRAQAAFIDCDYAIGLEAGFMRVPNAKSGYMNVSACAIHDGTDTHLG
                     LSSAFETPDKEVMRLVVEEGLNFAEAANRTGLDPDPELGKKSGVIGKLTGGKINRKEY
                     VQQSLVTALIHIDN"
     gene            19143..20051
                     /gene="atpG"
                     /locus_tag="VEA36_03595"
     CDS             19143..20051
                     /gene="atpG"
                     /locus_tag="VEA36_03595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HYF29417.1"
                     /translation="MALKDIKTKIKATERMHKVTRAMEAVSAVKMRKTQASALAGRPY
                     ARAAVSILSRLSATRHITGHPLAEHREVKKVALVIITSDKGLAGALNSGVLKKALEAL
                     EGRAKEEVIVYAYGRKGEEFFARRGYEIRKSIENKRDDVPLSVMEEIAAEVTKGFLAR
                     DFDRVVAVYSAFKSTFEQVPTWRRILPLSLGALTDIVSDITPAKGKWSEEEAPQAPAS
                     YEVEPSEEEVLGILIPRLVAVALYHMLLEGKASEHSARMVAMKNASDNSKEVVHDLNR
                     VYNKVRQAAITREVSEIVGGREALAS"
BASE COUNT         3926 a         6200 c         6369 g         3723 t
ORIGIN      
        1 tgaacggggg aaggcgtcct cgccggatcg catcctgccg accgtcgacg tcgccgtggt
       61 ggcgctccat ggcgcctacg gccaggatgg cgaggtgcag aagctccttg agcgcttcgg
      121 ggtagcgtac acgggagcgg acccttgggc ctgctacctt tctgcccaca agctcatggc
      181 aaagaagcat gtggaggagg tggggctcct gaccccgcgc taccacttca tcgatcaggt
      241 gacggagatc gaccgcatcg tgcacgaggc catccgcacc ttccaccagc cggtggtggt
      301 gaagccggtc gactccgggt cttccgtcgg ggtgtcgatc gtgggtgggt acgcgcccgt
      361 gcatcttgcg gtgacgaacc tcttcctcgc gggcaccaag ggggtcctca tcgaggagct
      421 cgtcaagggc accgaagcaa cggtgggcat cgtcgaagga ctgcgcgggg aggagctgta
      481 tgcgctcccg ccggtggaga tcgtgccgtc cgcggcgttc ttctcatacg aggcgaagta
      541 ttcggggcag agccgggaga tctgcccggg gcgcttcgac cggagggtga gcgaagcgtt
      601 gatggatgcg gcgcgcaggg cgcatgaggc gctcgggctc cggcactact cgcgctcgga
      661 cttcatcgtc tcgccgaagg gcatctacta cctcgagacg aacaacgccg ccgcggtcgg
      721 catgacgggg gaatcgctct ttccgaagtc cttagcggcc gttggggtgg ggctaccgga
      781 gtttctcgag cacctcatcg aacgcgcgaa acggcattag catggaggag ctctcgaaga
      841 agaagtgcaa gccctgcgag gacgacggct tccccgcgct caccaaggag caggccgggg
      901 atctcatgca gcacataccg gaatggacgc tctccgagcg cggcacgagc atcacccggc
      961 agttcccctt caaggatttc aaggaggcgc ttgcctttgc aaacaaggtg ggcgagctcg
     1021 cggacgagga gtggcaccat ccggacctca tcgttgcctg gggccgggtg gaggtcctcc
     1081 tgaccacgca ttcggtgaag gggctctccg aaaatgattt catcctggcc gcgaaaatag
     1141 acttgttaga gaaatagggg ccatggtata acagtgccta cggggagacc cgcgtttttc
     1201 gttcgggccg ttagctcagt tggtagagca cctcttttgc aaagaggggg tcgcaggttc
     1261 gaatcctgta cggtccactc tcgagaaatc ccttggaatc ccaggggatt tctctttgac
     1321 cgttgaggca ggttcgaact gcgatcccct cgactaggtt agaggagcct agtcaaaatc
     1381 ctcggtgagc ggcaggccaa gcccaagatc ctctgcatac gctgcgatcg cctcgtcgag
     1441 cacatcggca gcgacgtaga ggcgagcaag caacagagcc tccgggctca ccacgggctc
     1501 agccggggtg aagaagggtc ggttcataga actcgatatt agagctgaca aagatcgggt
     1561 gcgcacccag gtctagaatc tactaaggcg aaatagaacg cacggatgcg gaatattcgc
     1621 cctaggaata gcagaaattc aatcgatggt atccttactt catggacaat cttcatccac
     1681 aggtggcgaa acgcgtaggt ataaccacgc ttaaggacgg tatgggcatc gaagatagag
     1741 gcatactggg tgtcaatttt tacagctcct tcaccagcgc caacattcac ccctcaattg
     1801 aagatcgtat gcttacagta ggagtgagga aatcagccct cggtgagttg tatctgatct
     1861 taaagcaact tattgaagaa agtccgaggg aatacggctt tgaaattaaa gtagaggtcg
     1921 tagagtgatg tcggctgatc gcatccttgt atgtctacga tctacattct tcacggttgc
     1981 ccatctgacg aagaaaaggc aatgaatccg gagactcgca cctacgataa gcactggatt
     2041 ccgtggcttc gggcgaagct cgagtcggag ggtcgctcag tgaagacacc gctgatgcca
     2101 aatccctggg cgccagatta cgatgcgttc aagacagaat ttgagaagaa tgcgattggt
     2161 gagaaggatg tcctcgtcgg ccacagctgt ggcggtgcct ttctcgtgcg ttggctggga
     2221 gataccaaac aaaagatggc gaagttaatc cttgttgcac cctggaagat tcccgctcta
     2281 ggagacgagc tccgtaggaa gtactacggc tacgaaatcg atcggaccat cccagatcgt
     2341 gtgggtgaaa tcgttatatt cacttcagat gacgaagagg aagacgggaa gcggagcgcg
     2401 cacatattcc acgaagcact gggcggagat ctcgttgaac tccctaagca tggacacttc
     2461 acaatggggg acatgggcac ggtggagttt ccggagctgt accagaccgt cataacgtag
     2521 cctgtgttct ggcactcgcc acattgaaac cgggcttagg gaaggccgcc cgctttcatg
     2581 cggcgagtgc caacccgtca tcagtggacg tgagggatgt aggggacgtt ggggacttct
     2641 cactcaagtc gaagaccatc tctaccgagc gaccctccac gatcattttc acgagttcag
     2701 agaagttcgt tcgaacgtgc ccctgaagct gtccaccgca atattacatc gctatagccg
     2761 actttccttc tgcgtaattt ccacgtttag tcgatccaac tcggttttca cgctgtcata
     2821 ggcagcctgc tcagtggcaa gctcggctct agcagcagct gcctcttgac cgtctgcggg
     2881 aacagtctgg cctctcatcg cggctagctg ggtccgcagg cgccgaagtt catcaagatg
     2941 cgccacacga tctcgcgcac cattaagttt tgtttctgcc tgctgcaggg cgatttcaag
     3001 acgggtgcgc tcgcggtatt cagggaggag atccagcttt ccgaccgcaa cgcctcgatg
     3061 cctcatagct tgggcaagat tcgcctccgc ctcgtggttg gcatcgatgg actgctgtaa
     3121 tctcgcccta tcctcgggcg ttggcacacc cagtccaagt cgttctagaa aacccataca
     3181 tcaatcataa tgcccggcag tcaatgccag ggcaaggtgt gggataacgc tgaagggggg
     3241 ctatactttt actcataaaa ctaaacgatt agctatggct acctacgttg atggattcgt
     3301 gctcaccgtg ccgaagaagc agctcgctgc gtataagaag atcgcgaccg aggcccgcga
     3361 tatctggatg aagtatggtg cgctcgatta caaagagtgc gtcggcgagg acctgaaacc
     3421 caagcaggtg aagctcacct tcccgaagat gatagcggcg aagccgacgg aggtcgtgtt
     3481 cttctcattc attgtctata agtcaaaggc agaccggaac aagatcaaca agcaggtcat
     3541 ggcgcacttc gagaagaagt acggcaagga catgaagatg ccggtggata tgcggcgctt
     3601 tgcgtacggc gggttcacga gcctcgtgga ggcgaagaag aagtagtcga cgaaagcgcg
     3661 actatttgac gcgagccgtg tacgattttt agatcggtgg tatagtcctt ccgggcaaga
     3721 ccgccttggc tctataacca cgggggatac cgtgatccta gacatcgtca ccggcgacat
     3781 tactacgccg gagaatccgt ccgacatcat catcgggatg aacagccagc tctcggacgt
     3841 caccggtatc ggccttccct tcgtgaagga cgtcatcccg atgcgtcaga tccagctcgg
     3901 ctccgtcatc actttcgagt tcgacatcca ccggcgcctg cacatgctga tttgccacca
     3961 tctcggcaag ggcggctggg cgtacgccga ccggcacgtc cggtacggcc tcgaccacct
     4021 cgagtatctc gaccgtgagc agcaggccgg cggcagcgac cggacctact ccatcgtgaa
     4081 gatcggcacc gggcgtgtgg gcgtgcgcga cggtgcggac gctccggcgc tgcatcgcgc
     4141 gatggccgac agcttcctgc cgctgaccct cttcctctac aatcgcgaga cggtggaggc
     4201 ggtcacacgg gagagccgcg caccgctcgt tcccatctcg acatggagcc cggaccacgg
     4261 ccgccagcgc atccacgcac ctgcggtcgc catgacctag gtcgcgcacc accttcggca
     4321 accccgtcgg gtcccttcac aggggctcgg cgggtctttt tataatggtc gggccgccgg
     4381 gaattgaacc cggtctatac gcacccgaag cgtatgtact accggtatac tacggcccgg
     4441 tgggcgacac aggattcgaa cctgtgacct ttcgcgtgtg aagcgaacgc tctaaccaac
     4501 tgagctagtc gcccgtagtc cgcacgatac caaatgcgtg cgcagatggc catggatcat
     4561 cggtatatac tgatgaggta tgaaaaacac gctcgtgctt gcgctcctca tcgtgccgac
     4621 tctgatcatc ctcgatctcc tctggatcgg gctcatcgcc cagggcatct accaggacca
     4681 gatcgggtat ctcttggcga ccgaagtaac ctggtgggct gcgctcctct tctacgtcat
     4741 ctacggtgtc gcgcttgcgc acttcgtcgt gctgccggca atcaagcttg gggacatgcg
     4801 gcgcgcgctc tataacggcc tcctgttcgg cctcgctgcg ttcgctactt acgacctcac
     4861 gaacctcgcg acggtgcggg actggccggt ggcgctcacc ttcatcgata tggtgtacgg
     4921 gaccatcgtt gcgggcgtca cgtcggcaac ggcatatgta cttggaaaga aactcgtgag
     4981 agggtaatct agaatttcgt tcgcgggctg tagtataccg gtagtacgca tccatggggt
     5041 ggatgtagac cgggttcaat tcccggcagc ccgaccgatg aatagcgtta tgaacatccg
     5101 acatgcgttc ggatgtcgcc tcgcgtctat acttactgtg gtgagcgatt agcgatggct
     5161 aatcggaacc ctaactgatc cgttggggaa gggagggttg gcgcagacgc tgcgacaacc
     5221 ctcctacact tctgcctcca tcttcccgct agcggccccg cccgtatttc tccttcagga
     5281 gccgcacgtg ctcctcgtac gtcggcgcgc agtggaagcc gccgtactta tcatggaaat
     5341 agaagaggca atccgtcttc ttgggattca gggcggcgat caccgcctct gttgaagggt
     5401 tcgcgatggg gccgggcggc agtcccttat gccggtacgt gttgtagggc gattttatgt
     5461 acttgtcgtt cgggacgacc ttcggccacc agctcttggt gtccgtattg gtcgcctttg
     5521 catactgcag cgtcgcatcg atctggaggt tcatgcccgt gaagaggcgg ttccagatga
     5581 tgccggagat catgcgcatc tcggcggggt cccgcgtctc gcgctcgagc atggaggcga
     5641 tggtgagggc gtcgtccaag gggacgacct ccgccgtcga ggtgctgtac cgggagagga
     5701 tctcgcggga gaagcgctcg ttaaggaggg cctgcacgtc ctcgcgctcg gcgttcgtcg
     5761 ggacggcata ggtgccgggg acgagctcgc cctcggtcag agtcggcggg gtcgaatgga
     5821 gctgtttcag gaactcgcgg cgctcggcgg cgctccagcc gagcgtcttg ccgaaggcgg
     5881 ctgcgacctc ctcctcgcga tatcccggga gcaccgtgac gaaggccgtg tccgcgccga
     5941 ccgcgcttgc aagcgaccgg tagagccctg agttagcgat cgtggccgca agccaggtgt
     6001 aggccatatc gagcgcgcct gcggcggccg caagctcggg attgtcctcg gcgagcaggg
     6061 cctccacttc cgggtcctcg atgatgacgc gggcgatcgg gtcgaccgtg acggggaagt
     6121 cgggcttcgg gctctctgcg gtatggagat cgaccgcgag cacggagggg agcgcgaaga
     6181 agccgagcgc aagcaggagg gtggcgcccg cgaccggccc gatgctcttc ttctggatag
     6241 cgagcttctt gaagacgccc tggggcgcct tcttcgatga agctgccgga tgcggcatca
     6301 ggctcttggg gggagtgcca ggcactccag tagtgtagcg ggattcccgt cgttaggaaa
     6361 ctccccgctg cgtgcataag cgtgcgtgct tcagcatccc tgtggtaggg tctatcgcat
     6421 gcagcgcatc acgccgcgcc agtacacggg ggtggaccgc atcgtttcgg cgatcggctc
     6481 cgtcccctcg ctcgtcatcc actcgctcgc cttcgcgggg ttcttcctca tcgccgtctt
     6541 cggcatcgcc tcgtgggaga tcgtgctcct cgtgctcacg acgatcgtct cgctcgaagc
     6601 gatctacctc gccatcttca tccagatcac ggtgaaccgg catacccagt cgctgcgcga
     6661 agtcgaggag gacatcgacg agatccagga ggacgttgag gagctcgggg aggacgtgga
     6721 ggacatccag gaggacatcg aggagatcca ggaggatttc gaggagatga gcgacgagga
     6781 gaagggcgag cacctcatcc gcgaggagaa gaagcgggtg aagaaggtcg cggatgaggc
     6841 cgagctctac gagaagctca ccaaggacgt ggagcgggtg ctccgggatc tcgagaccct
     6901 gaagcgcgcc agtggggata agcgataggg taaaccgaac ggtatcggac tagactgggg
     6961 tatgcatatg ccccgcctcc tcccggccct cctcatcggg ttcatcgtgc ttctcgtgcc
     7021 gttcaaaagc gacgccatcc tcctgacctc cgacctttcc atcggctccc gcggcgcgga
     7081 ggtcacccag ctccagctca tcctcttcca gttcgggtac tccaccgcag caccgaacgg
     7141 ggtgtattcg gagacgaccg ccgatggggt gcgggcgctc cagaagaaat acgggcttgc
     7201 ggtgaccggg acgcttgacg cccggacgcg cgcgctcctg aatccattca ttagccgcaa
     7261 cccgacctcg ggcgctacgc ggcctgcggc cgcctcctcc gggatcgcgt caggcgggac
     7321 ggcagcgctc gggctcggct cctcgggccc tgcggtcctc gaggtccagc agaagctcgc
     7381 ggcgctcggg tacttcaagg agacgccgac ggggtacttc ggcccgatca cccgcgcggc
     7441 ggtcatcacc ttccaggctg cgaagggcct cgagcaggtg gggcaggtgg gtcccttgac
     7501 ccgcgcggcg ctcgccggcg cgggtcccgt ggtcgcgtcc tcgggaggca cgagctcgtc
     7561 gagcatcggg gtgacgcctg cggcgaccca gacgaagacc accggcgagc gccctcccgc
     7621 gctcaccttc cgcatcgatc gcaagttcgc tctcgtcggc gactacatct acctgaactg
     7681 gtcggcgaag cgcgccgata gatgcactgc atcgggcggt tggtccggcg agaagggcac
     7741 caggggcacg gactcatttg cagttgcggg cgatacgacg tacaccctca cctgcacggg
     7801 ctcgggcggc tcggtgacga agtccgtgtg ggtctcgacc acgctcgttg cgcgcaaggc
     7861 ggaggaaccc gaggagatcg ctgagacacc ggtcgtgacc gtaccgacca cgccgacgac
     7921 accgaccacg cctgctactc cgaccactcc tacgacgccg aagcccactc ccatcctgac
     7981 cacgctcccc ggaccctcca aaagctatca ggtgtatgcg ggctgcgagg cgcccgcgaa
     8041 gtcctatgcg cgcgtcatct atgtggatcc ggtgaacggc acggacgcag gcgatggttc
     8101 ggccgcggct cctttgaaga ccgcttcgca ggcccttgcg tcgaagaaga tcaagcccgg
     8161 cgaccacgtc gtgctcctac cgggtaacca cggccgcctc tacgcggacg gcgtgtacgg
     8221 cagcgccgag ttcaaggatg cggcgaactg gacctggctc gacttccagg agggcgcgta
     8281 catctccaat cttgacttcc ggaacggcat gtcccggtgg ctcatcacca acgcggaggc
     8341 ctccaaccag ttcagcacct ctgcacggac catgatcgcg acctccggcg gcatacattt
     8401 cgtctttgcc gacggcgaac tgtattccat caagaatgcc tcgagctgga gcgcgagcga
     8461 ttggctgaat aaggcggcga gcggtatttc gacgcgcaat acccgttgcg tttccattct
     8521 cgataacgac atcaggaacg tggccggagg catcacgctg cagatcgatg cgaacgtatc
     8581 agataacagc atcaaggggc tcgtggcgcg caacaccatc ttcggcaacc aggccgatgg
     8641 gctccggccg cttggctcgg acatcaccat gcgcgacaac cgggtcatcg aatccatgct
     8701 cgggtccgag cacggcgacg gcaaccacga cgacggcctc cagatcttcc cgatcggcag
     8761 ccatgttttc aacaacatcc gcatcgaggg caactggatc caggagtcga cggcttccgg
     8821 ccggccgctc atcggcgggc agcagggcat cgggcacttc ggcggccccc tgaagaacag
     8881 cgtcatccgg aacaacgtca tcctttcgag cgtctggcac ggcatctcga cctacgatcc
     8941 cgagaacgtg atcatcgagc acaatacggt cctcaatacc tccggcaaca agcgcgcgac
     9001 ctggatcgcg gcgtggaagg gcggcaaggc ggagaacccg accagcggcg tgatcgtgcg
     9061 caacaacatc gcgcactcct tcatcctcgg caatggtgtg gcgagctcgt ccaacatcgt
     9121 catcgcgccc gatgccccgg cattcgtgca gttcgatcat gtgaacggcc ggttcgacct
     9181 gcgcccgaag ccgggaggac ccttggacgg gaagaacgca ggcgcatccg gcgtctcatc
     9241 ggggctcgga tcccaggcag cctccgggaa tccgtatgca cagacggcgg cggcggcgca
     9301 ggcccttcca accctcgagc agctctacct ccagcttcaa aacctcctga atacgctgcg
     9361 gaattgatgc gggtggggat tagcggcacg gtacgcgcgc agtcctctag aatgaacgta
     9421 tgaatctcac ccgacccttc ttcctgttcc tcgttgccgc cgcactcttt atgggtgcgg
     9481 cgcaccatgc ccatgcggcc ctgcttgttt cggatctctc catcggctcg cgcggcgcgg
     9541 aggtcaccca gctccagctc atcctcttcc agttcgggta ctccaccgca gcaccgaacg
     9601 gggtgtactc ggaagcgacg gccgatgggg tgcgggcgct ccagaagaaa tacggactgc
     9661 cgcagactgg catcgtcgac gcgaaggttc gcggattcct gaacccgttc attgcgcgta
     9721 acccaacctc cggctcgacc cgtccggcgt cgccgaccgc aggatccgtg ggagggggaa
     9781 cggccgctct tgcgtttggc gcccgtggcg cggccgtgct cgagctccag cagaagctcg
     9841 ccgcgctcgg gtacttcaag gagacgccga cggggtactt cggcccgatc acccgcgcgg
     9901 cggttgccgc gttccaggca gctcgcggac tcgagaccgt cgggtacgtg ggcccgcgaa
     9961 cgcgcgcggc gcttgccgag gttcccaccg gaaccgctac tgcggccgga tccggcacat
    10021 cgccccgggt ctccggtacc accaccaccg gcgagcgtcc tcccgcgctc accttccgca
    10081 tcgatcgcaa gttcgccatc gtgggcgatt acgtatacct cagctggtcc gccaagcgcg
    10141 cggacacctg cacggcgtct ggcgcctggt ccggcgacaa gggcacgaag ggctcggatg
    10201 ccctcaccgt tgcgggggac gcgacctata cccttacctg cacgggctcg ggcggctcgg
    10261 tgacgaagtc cgtgtgggtc tcgacgacgc tcgttgcgcg caaggcggag gaacccgagg
    10321 atgtgacgtc gacgccagtg gcggtcacgc cgccgagctc gactccgggc tcctctgcgc
    10381 caccttcgcc caccccaacg ccgacaccgg ccccagtccc gaccccgctt cccgggccct
    10441 cgaagagcta ccaggtatat ccagggtgcg aggcgccggc tcggtcctac gcacgcgtcg
    10501 tctaccttga tccacggaac ggatcggaag cgggcgacgg atctgcggcg gcacccctta
    10561 agacgctcgc gacggcgctt gccgcgaaga agctgcgcgc gggcgaccac gtcatcctca
    10621 tgccgggcga gcacggcgcg ttcgtcggcg acaccagctt tagccaaaag ggactcttct
    10681 tcagggatgc gccgaactgg acctggttcg atttccagga aggcgcgacc atctcgaaat
    10741 ccgatctgag cggcttctcg cgcatcctga tcaccaaccc cgaggtgacg cccggtggca
    10801 tcctcgttac gggcggcagc caggtggtgg ttgccgatgg cgagttctac ctagcgcgga
    10861 acaccgcaga ctggacggcc gagaaatggc tcgcgcaagg gaagggcaac acactcatgt
    10921 cccgcaatac ccgctgcgtc tcgttcatcg ataacgagct gcgcaacatc ccgttcggga
    10981 tctccatatc ggtggatgga ctaccgacca gcgacaatag cgccagggcg ctcgtcgcgc
    11041 gcaataccat ctcccgcttc cggggggacg gcatccggcc gaacggctcc gacgtcatcg
    11101 tgcgggacaa ccgcatcgtc gattcgatgc tcggccgcga gcacggcgac ggcaatcatg
    11161 atgatggcat ccagctcttc gccctgaatg gccataggtt caagaacgtg cgcatcgaga
    11221 acaactggat ccaggagaca acgagcgcca cccgtcccta tatcggtacg ctgcagggca
    11281 tcggccactt cgatggggtg atcgaggatg tcgtcgtgcg caacaatgtc gtgctgatcg
    11341 gggcgtacca cggcatcatg ctcaactcgg tcggcggtac cgtcgagaac aataccgttg
    11401 cgtatgtcgg caccgaggga cgtaatacct ggatccgcat gggcgcatcg agccagaaca
    11461 ttacgttccg caataacgtt gcgcgcttct tcgagtatcc gacgagtgcg ggtcgaatcc
    11521 agatccgtgc cccgcagcag gatgcgatcg gcaacaacat cacggtgaag gacaatgaga
    11581 gcgctgcatt catgaagttc gatcccgcaa atggcgcctt cgacctgcgc ccgaagccag
    11641 gcggcccgct cgacgggaag ggtgtcggcg ctgcagggct tacctcgggc ctcggttcgc
    11701 aggcggtact caaggacccc tccgcacaga ccgcaggcgc aggcaatgcg ctcccgtcgc
    11761 tcgccgacct ctacgaacag ctcatgaatc tcctcaagac gattcgctaa cccatcacgc
    11821 catgcatacc cgcctcatcg ctctgctcat agccgcctgc atggtcatgc cggttccggc
    11881 gcatgcggtg acactcacct ccgacctctc catcggctcg cgcggcgccg aggtcaccca
    11941 gctccagctc atcctcttcc agttcgggta ctcgaccgca gcaccgaacg gggtgtattc
    12001 ggagacgacc gccgacgggg tgcgggcgct ccagaagaaa tacgggcttg cggtgaccgg
    12061 gacggttggt gcaaagaccc gtgcgcttct gaatccgttc atcgctcgca atcccacgtc
    12121 cggctcgacc cgtcctgcgc catctgcttc gggtgggggt tcccccctta tgctcggctc
    12181 ctcgggccct gcggtcctcg aggtccagca gaagctcgcg gcgctcgggt acttcaagga
    12241 gacgccgacg gggtacttcg gcccgatcac ccgcgcggcg gtcatcgcct tccaggccgc
    12301 caggggattg gagcaggtcg gctcggtcgg tcctctcacg cgtgcagcgc tcgcaggagc
    12361 aggaccggcc agcgcgacct cagggagcgt cagctcgagc tcgaccggcg tatcaagcgc
    12421 aagcacgcgg acagccacca ccggcgagcg ccctcccgcg ctcaccttcc gcatcgatcg
    12481 gaagttcgcg ctcgtgggcg attatgtgta cctcagttgg tcggccaagc gcgcggacac
    12541 ttgcacggcg tctggtggct ggaccggcaa caagggcgtg aagggatcgg aatcattttc
    12601 ggtccctggt gatactacct atacgctcac ctgtaccggt tccggtggtt cgattacgaa
    12661 gtcggtatgg gtatcgacca cacttgtggc gcgtaaggtg gaggagcccg aggaagtgac
    12721 cgagacgccg gttgtggtcg cgccagttac tccggcgaca ccagcgacac ctacgacccc
    12781 gagcaaccct gcattgccgg ctgttcctgc aacaccagcg gtaccaaaac ccccgatccc
    12841 aacgacgctt ccgggcccct ctaagaacta tcaggtgtac cagggttgcg aggccccagc
    12901 gaagagctat gcgcgcgtgg tttatattga tccgcagcat gggctcgata cgggcgacgg
    12961 ctcgtcggca gctccgcttc gcaccctcca ggcggcattt tccgctaaaa aactcagggc
    13021 tggagatcac gtggtcctta tgcccggtaa tcatggggag tataccggcg acggaagata
    13081 caaccagagc ggactcttta cgaccccgaa ctggatttgg ctcgatttcc aggaaggagc
    13141 cgtcatgggc cggcttgagc tccgcaacat ctcccgcatc ctcatcacga atcccgagtt
    13201 caatccctca gcaaaggtcg gccaaatgct cctcgtttcc ggcggaacgg atctggttgt
    13261 cgctgacggt gagttctatt ccataaagaa ctcgggcaac tggagcgcga gcgattggct
    13321 cggtaaaaca tcaagtggca tcttcgtgcg taatcaccgc tgcgcatcag tactggataa
    13381 caagctgagg aatgttgcat tcggcatcag cattagcacc gacggattcc cgacgagcga
    13441 taatgccgca agagcacttg tagcgcgaaa cgagattatc ggcttccagg gagacgggat
    13501 acgacccaat gcgtctgacg tgtccgtgcg tgataaccgc atcatagaat cgatgctcgg
    13561 tgccgcagac ggggacgcca accacgatga cggcattcag atattcgcgc tcaatggcca
    13621 tgagttcagc aatatccgga tcgagaataa ctggatccag gagacgacga gcggctcgcg
    13681 gccgatgatc ggctcgctcc agggcatcgg ccatttcgac ggcattatta aggattcggt
    13741 ggtgcgcaac aacgtggtgc tcgtgagcgc gtactggggc atcgcgctca gcgggccgaa
    13801 ggattcgatc atcgagaaga acaccgccgt gaactcagcc tcgttctcgc gcggcgtgcg
    13861 catcgccgcg aacgccacca aggccacctg gggctctgtc ccgcctcaga acaccattgt
    13921 ccgggacaac gtcgcgcagg tgttcttcac aacgaccggt acggtaaccg cgtcgaacaa
    13981 cgtggtcgtt ggccaggaca tgtcgaacgt gttcgtaaca ttcgacaagg cgaacggtac
    14041 gtacgatctc cggcctaagc cgggcgggcc gctcgacggg aagggtgccg gcgcatccgg
    14101 catctcctcg agcctcggct cgcaggcggt cctcagcgat tcctacgccc agaccgcggg
    14161 cgcggctaat gcactcccgt ccctcgctga tctctaccag cagctcgtag atctccttaa
    14221 aacgttgaaa taggtaatgg ctcagcttgt acttcatttg cggttcatgt agccttgatg
    14281 aagtgatccg cagtaactcc tccgttacct cactcgcgct tcctcgctac ctgaccgtag
    14341 cggctgcgct tgtgttcctc ttcgcaccgt cgtttgcgca ggcggcgtcg ctctccccct
    14401 cgaccctcac gaagctcgtg aacgcagagc gcaccgcagc cggactcacg gcgcttagca
    14461 ccaacgctgc attgacccgc gccgcgcagg cgaaggcgaa cgacatggcg gcgaagggct
    14521 acttcgcgca tacgacccca gatgggaagt ccttctcgca ctgggtggtg aatgctggct
    14581 atgcatacaa gagcgcgggc gagaacctcg cgcagggctt ctcgacggag tccggcaccg
    14641 tgggcgcttg gatgaactcc cccgggcacc gcacgaacat catggcgtcc aagttcagcg
    14701 agaccggcat cgcggtcgca agcggtacgt acaatggcaa gtcggtgatc ttcgtggtgc
    14761 agatgttcgg ggcaccgagc ggctcggcag gggcggtagc ggcaaagcct gcaccggcac
    14821 cgaaggtcgc cgcgcaggct gttcctaagg cgcgcgtact cggcgcgatc gagaagccga
    14881 cggcgctgcc gaacgagctc gaagcaaagg acgcttcacc ggagaagacg ccggtatcgc
    14941 ggaccgagat ggtcgcggcc acggccgagg cggatgcgca gggacgcctg ctgaccctcc
    15001 tcatggagct ccgggacctc ctccttgcgg cggcgggcat ggtccgcgcg taggggattt
    15061 ggagcgttcc agacgctctg gtaaactgga cggcatatgg gaggggtact gacccaggca
    15121 gggtcttttt tacatatggt gcttggcggc ggcattgcgc gcgcggccga ggatgcttcg
    15181 catgccgtcg agggaacgga gcattcctcg gcgatccatg tggtcctgaa ggccgaggag
    15241 gtgttcaacc tcttcgggtt tccgatcacc aactcgctcc tgctgacctg ggtgacgatg
    15301 gcagtactga tcgggtttgc cttctatttc cggagccgcc tcgcgctcat cccggggaag
    15361 ctccaggcgg gcgtcgagtg ggcattcgag gccgtcctga attatatgga ggagaccttg
    15421 gagagccggg agctcgcgcg ccggttcttt ccgctcatcg cgaccatctt cctcttcatc
    15481 ttggtggcga acgaactggc cttcctgccg ggcgtaggta cgtcgcttgg ggtggataac
    15541 gggcatgggg ggctcacgcc cctcttccgc gcgcctgcgg cggacctcaa caccacgctt
    15601 gcgctcgcca tcatctcgtt cctgacgatc gagatcaccg gggtgctcac cctgggcctt
    15661 ttgaagtacg gcgggaagtt tgttaatttt aagtcacccg ttgattttgc ggtgggcttg
    15721 atcgagctgg tctccaacct ggggcgcctc atctccttct ccttccgtct cttcggcaac
    15781 atcttcgcgg gtgaggtcat gatcctcgtg gcggcgttct tcctgccgta cctcctcccg
    15841 gtacccttga tggccttcga ggtcttcgtc gggttcatcc aggcggtggt gttcgcgatg
    15901 ctgaccctgt tcttcatcaa gctggcaatc atggaaccgc acggggagac tgcgcattag
    15961 tctctcggtg cggctccggg atggagcagt aattattcga actcacactt atctatggac
    16021 gtagaagcag caacacagct gggcatggcg ctcgccgccg gtctcggcgt tatcggccct
    16081 ggcatcggca tcgggctcat cggcggtggc gccatgcagg cgatcgggag gaacccggag
    16141 gcctcgggca agatcgtgtc gaacatgatc ctcgcgatcg tgttcaccga ggcgctcggc
    16201 atcctcgcgc tcgtagtcgc cttcatcatc aagttcgtcg gctaacggat catggacgca
    16261 ctcttcgccg ccttcggcat cgactggaga ctcctgctgg tacaggcggt caacttcggg
    16321 gtgctcttcg tggcgctcac ctacctcctc tacaagccca cgatgaagac cctggacgag
    16381 cgccgggcga aggtcgccaa gggcgtcgag gacgcgcagg cggccgaggc gaagctcgcg
    16441 gaggcggacg aggtggcgct cggggtgcgc aggggcgcgg agcaggaagc ggaaggaatc
    16501 gtcgcgtccg cgcgcgaatc ggcgagcgcc gagaaggcgc gcatcgtgaa ggaggcggag
    16561 ctgcgtgccg cggccgtcgc tgcagatgcg gacgcccgcg ccaaggaggc agccgccaag
    16621 gcgctccggg agagcgagaa ggagatcgcg cggctcacga tcctcgccgc cgagaaggcg
    16681 atgcgcacct agcatggaga aggtctacgc agaggcgctc gaacggtccg tccttaaggg
    16741 cggggacgag ggacagctct ttgcggggct catgcggcac ctgaaggccg tgggccgcct
    16801 gaagctcctc ccgggcatac tgcgggagct tcgcgcacgg cagtcgcggc ttgcgaagct
    16861 cgcgccctcg gtggaggtcg cgagtaccgc cgagaaggcc gtggcgctca aagaggcgaa
    16921 ggccgcaggc atcgaggcag cggacgccgt agtgaacgac gcgctcattt ccggatggcg
    16981 cgggagaagc ggggcgcagc ttgtcgaccg ttcgggcaag caggcgctga tcgagattta
    17041 taggaacatc gttacaaaat aatcatggag agcatcatca agcggatcga gagtgagatc
    17101 gcatcctacg tccccaaggg aggcacggag gagaccggca tcgtgcgcgc gaccggcgac
    17161 ggcatcgcgg agatcgacgg cctcgacaac gccatcatga cggagatggt gctcttcgac
    17221 ccgaccttcg accgcaccct cgaggaggcg ctgaaggacg ccgcgcccat ctacgggctc
    17281 gtcctcaacc tcgaggagga cggggtgcgc gccgtgatct tgggcgacgc atcgaaggtg
    17341 ttcgagggca tgctggtgcg ccgcgtgggt cgcctccttt ccatcccggt aggcgattcg
    17401 cttgttggcc gcgtggtgaa tccgctcggc gagccggtgg acggcaaggg cccgaccggg
    17461 gtgaaggaca tccgccccat cgagcgcgag gcctacggcg tgatcgaccg cgccccggtg
    17521 aataccccgc tccattcggg catcaaggcg atcgatgcca tgaccccgat cggccgcggc
    17581 cagcgccagc tcatcatcgg cgaccgcgga acgggcaaga ccacgattgc gattgatacg
    17641 attctcaacc agaagtcgga ggacacggcg acgcgcccca tctgcgtgta tgtggcgatc
    17701 ggccagaagg aatcgaagac cgcgaagatc gtggcgcagc tcgctgaggc aggcgccatg
    17761 gactacacca tcgtggtgac ggcgccggca tcctcgcctg ccgccttcca gttccttgcg
    17821 ccgtttgcgg gcgcgaccat cgccgagcac ttcatggagc agggcaagga tgcgctcgtc
    17881 atctatgacg acctctcgaa gcaggcggtg gcgtaccgtc agatgtcgct gctgcttcgg
    17941 cgcccgccgg gccgcgaggc gtatccgggc gacatcttct acctgcactc gcgcctgctt
    18001 gagcgcgcgg ccaagctctc caaggagaag ggcggcggct ccatcaccgc gctcccgatc
    18061 atcgagacgc aggagaacga cgtctcggcc tacatcccga cgaacgtcat ttccatcacc
    18121 gacgggcaga tcttcctcga gaccgatctc ttcaacaagg gacttcgtcc cgcgatgaac
    18181 gtgggcatct cggtgtcgcg cgtgggtagt gctgcgcaga cgaaagccat gaagaaggtg
    18241 tcgggcaaga tcaagctcga gctcgcccag ttccgcgagc tcgaggcctt catgcagttc
    18301 tcctccgacc tcgatgcgga caccaaaaag cgcatcgatg ccggccagcg catgaccgag
    18361 atgttgaagc agcccaacgg caagccgctg cccttcgaga tgcaggcggc agtcatcttc
    18421 gcagccacca acggatactt cgataccttc accccgtccg agaccgctgc ggccgaggag
    18481 cgcctccagg atttcctacg ccgtgggggg acgggcgtgc tcgagaagat ccgcaccagc
    18541 cgcgagattg ctgaggagac ggagaaggaa ctgcgcgagc tgctcgattc gttcgtcgag
    18601 cggtcgacat aagcatatga agatcgtggt cggctccagg aacccagcga aagtagaagc
    18661 ggtacgcgag attctcgcgg actatccgcg cttcaaggac gcggaaatcg ttgggatcga
    18721 agtcccgtca ggcgtgcccg atcaaccaac ctcgtttgag gaagtgacag ggggagcggt
    18781 taatcgggca caggcagcgt tcattgactg tgattacgct atcgggcttg aggccggatt
    18841 catgcgggtg ccgaatgcga agagcgggta catgaacgtg agtgcgtgcg cgatccacga
    18901 cggcaccgat acgcatctcg gactttcttc ggcatttgag acgccggaca aggaggtgat
    18961 gcggctcgtg gtcgaggagg gtctcaattt cgccgaagca gcgaaccgga ccgggctcga
    19021 tccggatcct gagctcggca agaaaagtgg ggtgatcggc aagctcactg gaggcaagat
    19081 caaccggaag gagtacgtgc agcagtctct ggtgaccgcg ctcattcaca tcgacaacta
    19141 atatggcgct gaaggacatc aagacgaaga tcaaggcgac cgagcgcatg cacaaggtga
    19201 cgcgcgccat ggaggcggtg tcagccgtga agatgaggaa gacgcaggcg tctgcccttg
    19261 cgggacgccc ctatgcgcgt gccgccgttt ccatactctc gcgcctttcc gcgacccgcc
    19321 acatcaccgg gcaccccttg gccgagcacc gggaggtgaa gaaggtcgcg ctcgtcatca
    19381 tcacgagcga taaggggctc gcgggtgcct tgaactcggg tgtgttgaag aaggcgctcg
    19441 aggcgctcga gggccgagcg aaggaggagg tcatcgtgta tgcctatggg cgcaagggcg
    19501 aggagttctt cgcgcgccgc gggtacgaga tccgcaagtc catcgagaac aagcgcgacg
    19561 acgtgccgct ctcggtcatg gaggagatcg cggccgaggt gacgaaggga ttcctcgcga
    19621 gggatttcga tcgcgtggtc gcggtctaca gcgcattcaa gtccaccttc gaacaggtcc
    19681 cgacctggcg gcgcatcctc ccgctttcct tgggggcgct gaccgatatc gtctcggata
    19741 ttacgcccgc caagggcaag tggagcgagg aggaggcgcc tcaagctcct gcctcctacg
    19801 aagtggagcc atcagaggag gaggtgctcg ggatactcat cccccggctc gtcgccgtcg
    19861 cgctctacca catgctcctt gagggcaagg cctccgagca ctcggcacgc atggtggcga
    19921 tgaagaacgc ctcggacaac tcgaaggagg tagtgcatga cctcaatcgc gtgtacaaca
    19981 aggtgcggca ggcggccatc acgcgggagg tgtcagagat cgtgggcgga agggaggcat
    20041 tagcaagtta acttttagta ctaactacga gcgtatgaac acaggaacca tcacagagat
    20101 catcgggccg gtcgtcgacg tgcacttcga gaaggatcgc ccggcgattc aggaggcgct
    20161 cgttatcgag aagggcgggg cgcacgaacg catcgtgctc gaggtggcaa gccatgtc
//
LOCUS       DASPKG010000088         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_393721, whole genome shotgun sequence.
ACCESSION   DASPKG010000088 DASPKG010000000
VERSION     DASPKG010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393721"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VEA36_03600"
     CDS             <1..759
                     /locus_tag="VEA36_03600"
                     /inference="COORDINATES: protein motif:HMM:NF025806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HYF29418.1"
                     /translation="YRVDGAKTLGEPVGLKGTRLEADYLFITCLSQHAELMESVTEAA
                     DVEVIDRMASPLAGSYVTLTKDQKMKGCVLANIGSETASIVIYDEGLPVSVKVFPMGS
                     SHITDDLALGFKVSLEDAERLKLGRLSGSMYPRKRVEEIITSRFRSIFDLIDKHLKQL
                     GRGRTLPAGIIIAGGGAGQGLITDIAKGALNLPSRVADMRLTTDVKVRDATWSVAYGL
                     ALWGLTGDTDTPKKKRGMKEFAEGLKEFFRQFLP"
     gene            842..1213
                     /locus_tag="VEA36_03605"
     CDS             842..1213
                     /locus_tag="VEA36_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29419.1"
                     /translation="MQKLRKLGTTVLTVIGALSVAGFANDLAATARIRAQPDNPLTRM
                     EQALERCHALKQPYVEICMSDTWNRLRDDMDFYIVSCDISKVGHFEFGLNEYQRPFLG
                     TPIFYNVPFYFFLGSCHSWPG"
     gene            1503..>2005
                     /locus_tag="VEA36_03610"
     CDS             1503..>2005
                     /locus_tag="VEA36_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29420.1"
                     /translation="MSKRVDTEIETFARIRVVGVGGSGCNAVNHMVNSKVKGVEFIAV
                     NSDAQDLHRSLAKKKIHIGKNLTKGLGTGMNPELGKRAAEETRQEIQEALQGSDMVFI
                     TCGMGGGTGTGAAPVVAKIAKEIGALTVGVVTRLQAGRGVLRAARVVRPLPPGLGLHP
                     APHPLGRR"
BASE COUNT          395 a          610 c          609 g          391 t
ORIGIN      
        1 taccgggtgg atggggcgaa gacgctcggg gagccggtcg gtctcaaggg gacgcgcctt
       61 gaagcggact acctcttcat cacctgcctt tcccagcatg cagagctcat ggaaagtgtg
      121 acggaggccg ccgacgttga ggtcatcgac cgcatggcgt cccccttagc aggctcctac
      181 gtgaccctca ccaaggacca gaagatgaag ggatgcgtgc tcgcgaacat cggctcggag
      241 acggcgtcca tcgtcatcta cgacgagggg ctccccgtct cggtgaaggt cttccccatg
      301 ggctcctcgc atattaccga tgatctcgcg ctcgggttca aggtgtcgct tgaggacgcg
      361 gagcgcctca agctcggccg actctccgga agcatgtacc cccgcaagcg cgtcgaggag
      421 atcatcacga gccggttccg cagtatcttc gatttgatag acaaacacct gaagcagctc
      481 gggcgcggca ggaccctgcc ggccgggatc atcatcgcgg gcggaggcgc cggccagggg
      541 ctcatcacgg atatcgcgaa gggcgccttg aacctccctt cccgggtcgc tgacatgcgc
      601 ctcacgaccg acgtgaaggt ccgggacgcc acctggtccg ttgcctatgg cctcgccctc
      661 tggggcctca ccggagacac ggatacgccc aagaagaagc gcggcatgaa ggagttcgcg
      721 gaggggctga aggagttctt ccggcagttc ttgccgtagt tggttggtgt ttctatacaa
      781 ctgtgttagc ttttgtccag cttccggata tcaatcgttg aacctttgag ggagagtaga
      841 gatgcagaag cttcgtaagc tcgggacgac ggtcctgacg gtgatcggcg cgctctcggt
      901 ggcgggcttc gcgaatgatc tggccgccac ggcccgcatc cgcgcacaac cggataatcc
      961 gctgacccgg atggagcagg cgctcgaacg gtgccatgcg ctcaagcagc cgtacgtgga
     1021 gatctgcatg agcgacacct ggaaccgact cagggacgac atggacttct atatcgtctc
     1081 ctgcgatatc agcaaggtcg gccacttcga gttcggcctg aacgagtacc aacggccttt
     1141 cctggggacg ccgatcttct acaacgtgcc cttctatttc ttcctcggca gctgccatag
     1201 ctggccgggc tagcgtcctg actgcggcga ccgaaggaga gcgaatcggc accaaaagtt
     1261 gacgttcgaa ttccgtatcg ccaaacgagc cctcggtcca tagaccgggg gttcttcgta
     1321 cctcgtagtc atcgatacag tcataaaagt gagcaacgag gccgttgtta gataattttt
     1381 gggaactctc ggcaaatttt cacacacaag caagacacgt ctgaaaaatt tgtcgatatt
     1441 tgcatgccgt ccccagctgg tatcatggcc ccagcctttt tatccggctc tagcttcctc
     1501 atatgtccaa gcgcgtagat acagagatcg agacgttcgc acgcatccgc gtggtcggcg
     1561 tcggtggttc cggctgcaac gccgtcaacc acatggtgaa ttcgaaagtg aagggcgtag
     1621 agttcatcgc cgtgaactcg gacgcccagg accttcaccg gtccctcgcg aagaagaaga
     1681 tccacatcgg caagaacctc accaaggggc tcggtaccgg catgaacccg gagctcggca
     1741 agcgcgcggc cgaggagacg cggcaggaga tccaggaagc gctccagggc tcggacatgg
     1801 tgttcatcac ctgcggcatg ggcggcggca cgggtaccgg tgcggctcct gtcgtcgcga
     1861 agatcgcgaa ggagatcggc gcactcacgg tcggcgtcgt cacgcgcctt caagccggtc
     1921 gaggcgttct tcgagccgct cgtgtcgttc gcccgctacc tcccggcctc ggccttcatc
     1981 ccgctcctca tcctctgggc cggcg
//
LOCUS       DASPKG010000089         4314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1609676, whole genome shotgun sequence.
ACCESSION   DASPKG010000089 DASPKG010000000
VERSION     DASPKG010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609676"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..398
                     /locus_tag="VEA36_03615"
     CDS             156..398
                     /locus_tag="VEA36_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29421.1"
                     /translation="MYATGTAFTIGPALSFLDPGKVVSVIAWVLFVLWAIYTAIAAYH
                     WLRYSHQSSVAIPALIVHFIVSAALALFAVSGFAPS"
     gene            401..967
                     /locus_tag="VEA36_03620"
     CDS             401..967
                     /locus_tag="VEA36_03620"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HYF29422.1"
                     /translation="MEEFALEPGEKVTLSVRKHWFLFLVQLLPFALLAYLPLIFPDFI
                     EGVSNAAPQAALGLQGFTVENPWFRLGLGAWWLILWILAFNVFTSYFLNLWIITTTRI
                     VHIRQYGFFSRQVSSFLLSHIQDVTTTVDGFFATLLNYGSVRAETAGDASACFRMTGM
                     PDPTGIRDLIMKEIADLHLSDSPPNPKV"
     gene            988..2262
                     /gene="hisS"
                     /locus_tag="VEA36_03625"
     CDS             988..2262
                     /gene="hisS"
                     /locus_tag="VEA36_03625"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HYF29423.1"
                     /translation="MSDKEPLSAEPYKGVRDFYPEDWTRLQACFEVIRKVLRSYGFEE
                     YNASPLERAELYESKTSEEIVNEQTYTFTDRGDRRVTLRPEMTPTLARMVAAKRRELV
                     YPLRWFSIGNRFRYERPQKGRLREFYQVDVDIIGAADAKAEGEAVVMAYEILKAFGAQ
                     NADFTIKANSRKLLNAACEAVGLSADETTAYLGLVDRRNKMDAEKFEAARGEYRRNGK
                     DPLEVIEAHDTDAVEAEYQQLMGLIEALKARGMDNVVFDPSVVRGFLYYTGLVFEVFD
                     TNPENPRALIGGGRYDGLVALFGGDPIPAVGFAMGDVTLIDFLTTHNLLPSESSAPEL
                     YIGTPSEEDIGLAQGFAEIVRTAGVRAMVNITDRSLSDQIREAAKRGIRYFAAYGAGE
                     EESGTVRLKNLESGFEEERPIADVAAAIRSGS"
     gene            2265..2843
                     /locus_tag="VEA36_03630"
     CDS             2265..2843
                     /locus_tag="VEA36_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HYF29424.1"
                     /translation="MREITFDIETANAFPTFSRSDYAQLELSVVAIHDSQTDTYTAYT
                     KEELPQLWPILEKADVLIGFNSISFDTPILNKYYPGDLTKIRPLDLLVEVYNALGRRL
                     RLQSLAEATLGVGKSADGLKAVEWWQQGLVEKVKEYCIQDVRVTRGLYDYALEHGKLR
                     YKDIRDLRDIKLDVSKWRSEGPASAFTHTLPF"
     gene            2864..3556
                     /locus_tag="VEA36_03635"
     CDS             2864..3556
                     /locus_tag="VEA36_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYF29425.1"
                     /translation="MDNPTRSPWLIPSAIIIAGVILALTVFVIRSGSAGPPATPMPEM
                     IEPVAVADHIIGNPEAPVTIVEYSDIDSDDGKSFHAALAQLMTEYAPDGKVALVYRHL
                     PLTTVHEFAQRHAEAAECAASLGRADSFWRFIDQAHTLAPGGAELNPTSYPSIAAALG
                     IEEGTFTECVNSGRFSAKVRAQAHDALGAGAEGAPFTVLLIKGAKPVAINGALPYTAL
                     KELVDRSLAKVK"
     gene            3618..3806
                     /locus_tag="VEA36_03640"
     CDS             3618..3806
                     /locus_tag="VEA36_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29426.1"
                     /translation="MLEGLGWRKNPSEKRMFVDRKTGAPAEKIVQGGQGEVIGLAKTP
                     TDEIDLVQKNEEQWKEAA"
     gene            complement(3850..>4314)
                     /locus_tag="VEA36_03645"
     CDS             complement(3850..>4314)
                     /locus_tag="VEA36_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HYF29427.1"
                     /translation="GDTFDALLKHARASLASYVHEADSRIVLSIEEEFKASQGEVVLS
                     GRVDALFEGTGGECIVDFKTTGTIDKKKKEEYESQLAFYDHLLRENGRNPVEGLIIQI
                     GEDKVVEHAFPLTPELREAFKDNLTAVIAELENGKWRAGEPSEYDGLLTLFK"
BASE COUNT          785 a         1360 c         1347 g          822 t
ORIGIN      
        1 agccgggagg cgagctctgg atggagtgcg acgtggataa catcgagaag gccaggcggc
       61 tcgtgctcga ggacggcgct cgccgggccg acatacgcac cgaccagtat ggccgggccc
      121 gtaccctcgt gggatactac ccctaagctc atcctatgta cgcgaccggc actgccttca
      181 cgatcggccc cgcgctctcc ttcctcgatc ccggcaaggt ggtatcggtc attgcctggg
      241 tactgttcgt gctctgggcg atctatacgg cgatcgcggc gtaccactgg ctccggtaca
      301 gccaccagtc ctccgtcgcc atcccggcct tgatcgtgca cttcatcgtc tcggccgcgc
      361 tcgccctatt cgcggtgtcg ggcttcgcgc cctcgtaggt atggaggaat tcgcgcttga
      421 gcccggggag aaggtcacgc tgtcggtgcg aaagcactgg ttcctcttcc tcgtgcagct
      481 ccttcccttc gcccttctcg cctacctccc gctcatcttc ccggacttca tcgagggggt
      541 atctaatgcc gcgccgcagg cggcgctcgg gctccagggc ttcaccgtgg agaatccctg
      601 gttccggctc ggactcgggg cgtggtggct catcctttgg atactcgcct tcaatgtctt
      661 tacctcctat ttcttgaatc tgtggatcat caccacgacg cgcatcgtgc atatccgcca
      721 gtacgggttc ttctcccggc aggtatcgag cttcctcctt tcgcacatcc aggacgtgac
      781 gacgacggtg gatggcttct tcgcgacgct cctgaactac ggctcggtgc gcgccgagac
      841 cgcaggcgac gcttccgcct gcttccggat gacggggatg ccggacccga ccgggatccg
      901 tgacctcatc atgaaggaga tcgcggatct gcacctgtcg gatagcccgc cgaatcccaa
      961 ggtctagata tctggtatcc tagccctatg agcgacaagg agccgctgtc tgccgagccg
     1021 tacaagggcg tacgtgattt ctatcccgag gactggaccc gcctccaggc ctgcttcgag
     1081 gtgatccgca aggtgctgcg ctcctacggc ttcgaggaat acaacgcctc ccccttggag
     1141 cgcgccgagc tctatgagag caagacctcc gaagagatcg tgaacgagca gacgtatacc
     1201 ttcacggacc gcggagatag gcgggtgacg ctccgtcccg agatgacccc gaccctcgcc
     1261 cgcatggtgg ccgcaaagcg ccgcgagctc gtgtatccct tgcgctggtt ctcgatcggg
     1321 aaccggttcc ggtacgagcg gccgcagaaa ggacggctcc gcgagttcta ccaggtggac
     1381 gtggacatta tcggggcagc ggacgcgaag gcggagggcg aggccgtcgt gatggcgtac
     1441 gaaatattga aggccttcgg cgcgcagaat gcggacttca ccatcaaggc gaactcgagg
     1501 aagctcctga atgcggcctg cgaagcggtc gggctctccg ccgacgagac gaccgcgtac
     1561 ctcgggctcg tggaccgcag gaacaagatg gacgccgaga agttcgaggc agcgcgcggg
     1621 gagtaccgcc ggaacgggaa ggacccgctc gaggtcatcg aggcgcatga tactgatgcg
     1681 gtcgaggctg aataccagca gctcatgggg ctcatcgaag cgcttaaggc gcgtggcatg
     1741 gacaacgtcg tcttcgatcc ctcggtcgtg cgcggcttcc tctactatac gggcctcgtc
     1801 ttcgaggtct tcgatacgaa ccctgagaat ccccgtgcgc tcatcggggg cggccgctac
     1861 gacgggctcg ttgccctctt tggcggcgac cccatccctg ccgtcggctt tgcgatgggc
     1921 gacgtgaccc tcatcgattt ccttaccacc cataacctcc tcccgagcga gtcgagcgcg
     1981 cccgagctgt atatcgggac accttcagag gaggatatcg gtctagcgca gggattcgca
     2041 gagatcgtgc gcacggctgg cgtgcgcgcc atggtgaaca tcacggaccg atccctgtcg
     2101 gaccagatta gggaggccgc caagcgcggc atccggtact tcgccgcgta cggggcgggc
     2161 gaagaggaat cgggcacggt gcggctgaag aacctcgaga gcgggttcga ggaagagcgt
     2221 cccattgccg atgttgctgc cgccatccgt tcaggttcct agctatgcgc gagatcacgt
     2281 tcgatatcga gacggccaac gccttcccta ccttttcccg gagcgactac gcgcagctcg
     2341 agctctccgt ggtcgcgatc cacgattcgc agaccgatac ctataccgcc tacaccaagg
     2401 aggagctccc ccagctctgg cccatcctcg agaaggcgga tgtgctcatc gggttcaact
     2461 ccatctcctt cgataccccc atcctgaaca agtactatcc cggggacctc acgaagatcc
     2521 gcccgctcga tctcctcgtc gaggtgtaca acgcgctcgg gaggaggctt cggctccagt
     2581 ccctcgccga ggcgacgctt ggcgtcggga agtcggcgga tgggctcaag gcggtggagt
     2641 ggtggcagca gggtctcgtc gagaaggtga aggagtactg catccaggac gtgcgcgtca
     2701 cccgcggcct ctacgattac gcgctcgagc acgggaagct ccggtacaag gacatccggg
     2761 atctccggga tatcaagctc gacgtctcga aatggcggag cgagggcccg gcgagcgcgt
     2821 tcacgcatac gctgcccttc tagggcggtg ctaggatgag ggcatggata atccaacccg
     2881 gagcccctgg ctcatcccgt cggcgatcat catcgcgggc gtgatcctcg cgctgaccgt
     2941 attcgttatc cgatcgggca gcgccgggcc gcccgcgacc ccgatgcccg agatgatcga
     3001 gccggtcgcg gttgcggacc acatcatcgg gaaccccgag gcccctgtca cgatcgtgga
     3061 gtattccgat atcgactcgg acgacggcaa gagcttccat gcggcattgg cccagctcat
     3121 gacggaatac gcccctgacg ggaaggtcgc gctcgtgtat cgccacctcc cccttacgac
     3181 cgtccatgag ttcgcgcagc ggcatgcgga ggccgccgag tgcgcggcat ccctcgggcg
     3241 cgccgattcc ttctggcgct tcattgacca ggcgcacacc cttgcgccgg gcggcgcgga
     3301 gctcaatccc acgagctatc cctcgatcgc ggctgcgctc gggatagaag aagggacgtt
     3361 cacggaatgc gtgaactcgg gccgcttctc cgcgaaggtg cgcgcgcagg cgcatgacgc
     3421 cctaggggcc ggtgccgagg gcgcgccctt caccgtgctg ctgatcaagg gcgcaaagcc
     3481 ggtcgcgatc aacggcgcgc tgccgtatac ggcactcaag gagctcgtgg atcggtcgct
     3541 cgcgaaggtg aagtagcgtc tggacagttt ttgtagtaat gggagaattg ctgcgtagca
     3601 atagccgagt ctaaaatatg cttgaaggat tggggtggag aaagaacccg agcgagaagc
     3661 gcatgttcgt tgaccggaag actggtgcgc ctgctgaaaa gatcgtgcag ggaggccagg
     3721 gcgaggtcat cggcctcgcg aagacgccaa cggatgaaat cgatctcgtt cagaagaacg
     3781 aggagcagtg gaaagaggct gcgtagagcg gctcttcagc aaagcgcccg cgccgcgggc
     3841 gctttcgctc tatttgaaga gtgtgaggag cccatcgtat tcggacggct cacctgccct
     3901 ccacttccca ttctctaatt ccgcaatgac ggccgtaagg ttgtctttga atgcttctct
     3961 aagctccggc gtgagcggga acgcatgctc gaccaccttg tcctccccga tctggatgat
     4021 gagcccctcg acggggttcc ggccgttttc ccgcaggagg tggtcataga acgcgagctg
     4081 ggattcatac tcctccttct ttttcttatc gatggtcccg gtggtcttga aatcgacgat
     4141 gcactccccg ccggtcccct cgaagagcgc atccacgcgc cccgagagca ccacctcgcc
     4201 ctggcttgcc ttgaattcct cttcgatgct aagcacgatg cgcgaatcgg cctcatgtac
     4261 gtaggacgcg agggaggcgc gggcatgctt caggagcgca tcgaaggtat cgcc
//
LOCUS       DASPKG010000090         5823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_125264, whole genome shotgun sequence.
ACCESSION   DASPKG010000090 DASPKG010000000
VERSION     DASPKG010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125264"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1373)
                     /gene="pgk"
                     /locus_tag="VEA36_03650"
     CDS             complement(240..1373)
                     /gene="pgk"
                     /locus_tag="VEA36_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HYF29428.1"
                     /translation="MRTLRDIRHLENVPVLVRAALNVPVADGTVTNTFRLRSALPTIE
                     YLRSRHARVILIGHLGDKSTESFLPVYEALKGFIPGIKFCDSAVGKAAREAVRDLVPG
                     DVLLLENLRRHAGEKGNDPAFAAELASLADAFVMDSFDVCHRTHASVVGVPELLPSYA
                     GLQVEREVKELSKGLRPRRPALAIIGGAKFATKEALLSVLLDSYDHVFVGGALANDFM
                     QALGQSVGTSLVSGADHAAIEKLLHNQKLRLPIDYVVAPLGSEREEGHLAGVTIVSNT
                     EAILDNGPKTLEMLGALVADAKTILWNGPLGNYEKGFTDGTEALARLIATSKAHSIVG
                     GGDTIAAIEKLGLDEHFSFISTGGGAMLDFLADGSLPGIEALQ"
     gene            complement(1411..1626)
                     /locus_tag="VEA36_03655"
     CDS             complement(1411..1626)
                     /locus_tag="VEA36_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29429.1"
                     /translation="MHAHYAARVYGWHWRQEIRAAGSVHAARKRFKGIRKERPGIMLP
                     DFCERHELADLITVWWREQKAKRQAAE"
     gene            complement(1789..2529)
                     /gene="tpiA"
                     /locus_tag="VEA36_03660"
     CDS             complement(1789..2529)
                     /gene="tpiA"
                     /locus_tag="VEA36_03660"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HYF29430.1"
                     /translation="MLIVSNWKAYVEKLDKAKELVATANRVARKGGHELVLAPPAPFL
                     AFLASGKTRAKFAAQNLSLVTAGAHTGEVTAAAVVGAGASYAIVGHSERRAAGETDAE
                     VLEKVRHALAHKLTPIVCVGERERDADARYLSFVREQLAAVFSPLTPKERLAVVVAYE
                     PVWAIGKGAGDAITTLDLAEMIAYIRKVLGDYIPGRGPQKVRILYGGSVEPANIRDLA
                     GGGGIDGFLIGHASADVPTYAALLKALS"
     gene            complement(2568..3983)
                     /locus_tag="VEA36_03665"
     CDS             complement(2568..3983)
                     /locus_tag="VEA36_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29431.1"
                     /translation="MQRPPRGYLLLLGIVFGAIFLMVLGSLASFVLTTNKAQTASTDR
                     AKAFSIAEAGLEYYRWFLAHNPGDLTNGTGNPGPYAIPYEDPESDVAGTYTLDIQGNT
                     SCGQVTSIDIDSTGQPSGSTVNRTLTVRYTRPTVALYSYVLNDSVWAGADRVINGPYH
                     SNGGIHMDGTANAPVTSSVATWSCTGSYGCSPTQTVAGVWGSGPNQTLWSFPVPQVDF
                     AAISADFGTLKATAIASGAYLPRYSNGASNSSAYWRGYHLRFNADGTITVWRVTHATP
                     LSNVPINSSDGSTDRILIQNEVAHSTIALPAGCKLLFVEDHVWIEGTVGEKVTVVSAN
                     VANPGVETNAYLRGNLVYGTTDGTDGLTLIAERNILIAPDSPNNMTLNGIFIAQGGAF
                     GRNLYACPSAYEPKGTLTIRGTTVSNRRTGTKWVNGCGGGNAAGYQTRIDAFDRQLAT
                     DPPAFTPITSSDYQFVDWREE"
     gene            complement(3993..4568)
                     /locus_tag="VEA36_03670"
     CDS             complement(3993..4568)
                     /locus_tag="VEA36_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HYF29432.1"
                     /translation="MRGFTLVETMVVVAIVVTAGVALMGSIQYFYRSNAYILEQTAAL
                     ESARRGHAFTLQNIREASYGDDGSYLITDAQSNTITFYADIDKDGGIERIRAYLSNGT
                     LYRGITNSTGNPATYTGQPETIETIAVAVRNTTPIFRYYGADGLELTSPIDLAEIVSV
                     GVRLDVDLNPTRAPNVFTLSGTATMRNLPTE"
     gene            complement(4570..>5823)
                     /locus_tag="VEA36_03675"
     CDS             complement(4570..>5823)
                     /locus_tag="VEA36_03675"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYF29433.1"
                     /translation="TLQIIVVDALGGPVAGAEVHIENPSTVPAVDLTTFTNAEGLVYL
                     PGALPSTDYRIETTKSGYSTATTYARDATNQNPTPGYLTVVENQTTTGTFAIDLLADL
                     TLRTFSPPQTLSFADTFADLSQIAEAVNVGVSAGALALVDSGMGYPGSGHAVSVAVTP
                     TALHEWGELNATLNPSASTEALIQVVDASGAVLPNAVLPGNEAGFDTFPVSLAGIATS
                     TYPTLKLRANLTSTASAETPELRDWSLSYRRGATPLPNIAFTLRGAKTVGSTGAGAAL
                     YKTTVADSTNGSGVKALSLEWDAYELTLNGYDVVEACPSPGYALAPGSATDSSLYLGA
                     NTTNMLLVTVKDNTGAVVPGADVTLSRTGFSQTVETTSCGTAYFGNLTSSSAYTVSIA
                     KAGYTTANFANVNVAGHIFYVATFE"
BASE COUNT          948 a         1882 c         1960 g         1033 t
ORIGIN      
        1 aacgaaggag aactcggtgg tgatcttcgt gggcgagatg tcccgcatcg cgcgcgggca
       61 cggattccag acgctgacct cggggtgcgc gaggtcctcg gcggtgatga gcacgccctt
      121 gtgctcccat ggcgccgcgc agagattgcg gtgatggccg attgaccaga gcttcacgat
      181 cttattcctt gtgctgtggg atcttaccct acagtacgca ggaaggacgc cgagtcaacc
      241 tactggagtg cctcgatccc cggcaacgac ccgtccgcaa ggaagtcgag catcgcgccg
      301 ccgccggtcg agatgaacga gaagtgctcg tcgagcccga gcttctcgat cgcggcgatg
      361 gtgtcgcccc cgccaacgat cgagtgcgcc tttgatgtgg cgatcaaccg cgcgagcgcc
      421 tcggtaccgt ccgtgaaccc cttctcgtaa ttccccaggg gaccgttcca gaggatggtc
      481 ttcgcgtcgg cgacgagcgc gccgagcatc tccagcgtct tcggcccgtt atcgaggatg
      541 gcctcggtgt tcgagacgat ggtcacgccc gcaagatgcc cctcctcgcg ctcgctccct
      601 aagggcgcga cgacgtaatc gatcggaagc cggagcttct ggttatgcaa aagcttctcg
      661 atggccgcgt ggtcggcgcc cgagacgagc gacgttccga ccgactgccc caaggcctgc
      721 atgaaatcgt tggcgagcgc gccgcctacg aacacgtgat cgtaggaatc gaggaggacg
      781 gagagcaggg cctccttcgt cgcgaacttc gcgcccccga tgatcgcgag cgcggggcgc
      841 ctggggcgca gccccttcga gagctccttc acctcgcgct ccacctggag ccccgcgtag
      901 ctcggcagga gctccggcac gccgacgacc gatgcatgcg tgcggtggca gacgtcgaat
      961 gagtccatca cgaacgcgtc ggcgagcgag gcgagctcgg cggcgaatgc cgggtcgttg
     1021 cccttctcgc ccgcgtgccg ccggaggttc tcgaggagga gcacgtcgcc cgggacgaga
     1081 tcccgcaccg cttcgcgcgc cgccttcccg acggccgaat cgcagaactt gatgcccggg
     1141 atgaacccct ttaatgcttc gtagaccggc aggaagcttt cggtgctctt gtcgcccagg
     1201 tgcccgatca ggatcacccg cgcatggcgg gaccgcaggt actcgatcgt cgggagcgcg
     1261 ctgcgcaacc ggaaggtatt cgtcacggtc ccatccgcga ccgggacgtt gagcgcggcg
     1321 cgcacgagca ccggcacatt ctcgagatgg cggatatcgc ggagcgtacg catgcatgca
     1381 tcataacgca gatgcgaaaa gcggcgggag ttactccgcc gcctgccgtt tggccttctg
     1441 ttcccgccac cacaccgtga tgagatctgc gagctcatga cgctcgcaga aatccggcag
     1501 cattatgccg ggacgttcct tgcgaatgcc tttgaagcgc ttgcgtgcgg catggaccga
     1561 cccggccgca cgaatctcct gccgccagtg ccatccatag acccgtgctg catagtgcgc
     1621 gtgcatgagt gtcggccagg tagtgccgtc ccaggtaacg gggcctggga acgctttgtg
     1681 ggcgcgcttg cataccgcgc gtaaccgtcg gacctgttca cgaggctcaa tcatggccaa
     1741 cccctcctct ggggagcacc ataccgcacg aactacccgt tcagcaactt atgaaagcgc
     1801 cttcaggagc gccgcatacg tcggcacgtc cgcgctcgca tgcccgatca aaaagccatc
     1861 gatgcccccg ccgcccgcaa gatcccgaat gttcgccggc tccaccgatc ccccgtacaa
     1921 gatacggacc ttctggggac ctcggccggg aatgtagtcc ccgagaacct ttctgatgta
     1981 ggcgatcatc tcggcgagat cgagcgtggt gatcgcatcg cccgccccct tgccgatggc
     2041 ccagaccggc tcgtaggcga cgacgaccgc gagccgctcc ttgggtgtga gaggtgaaaa
     2101 gaccgccgcg agctgctcac gcacgaagct gagataccgc gcatccgcat cccgctcgcg
     2161 ctcgcccacg cagacgatcg gggtgagctt gtgcgcgagc gcatggcgga ccttctcgag
     2221 cacctcggcg tccgtctcgc ccgcggcacg gcgctcggag tgcccgacga tggcgtagct
     2281 tgcgccggcg ccgaccacag ccgcagccgt cacctcgccg gtgtgcgcgc ctgcggttac
     2341 caaggacaga ttttgggcgg cgaacttcgc cctcgtcttg cccgaagcca ggaacgcgag
     2401 aaacggcgcg ggcggcgcaa gcacgagctc gtgaccgccc ttcctcgcga cccggtttgc
     2461 tgttgcgacg agctccttcg ccttatccag cttctccacg tacgccttcc agttcgagac
     2521 gatcaacata ggttccatga taccaggccc gcccgaaggg ctcggtatta ctcctcccgc
     2581 caatccacga actggtaatc gctgctcgtg atcggggtga aggcgggcgg gtcggtagcg
     2641 agctggcgat cgaacgcatc gatgcgcgtc tggtagccgg cggcgttccc gcccccgcag
     2701 ccattgaccc acttcgtccc cgtacggcgg ttcgagaccg tggtaccgcg gatcgtgagc
     2761 gttccctttg gttcgtacgc ggacggacag gcgtagaggt tgcggccgaa ggcgccgccc
     2821 tgcgcgatga agatgccgtt taaggtcatg ttgttcggag agtccggcgc gatgagaatg
     2881 ttgcgctcgg cgatgagcgt gaggccgtcg gtgccatcgg tcgtgccgta gacgaggttg
     2941 ccgcgcaggt acgcgttcgt ctccacgccc gggttcgcga cgttcgccga gaccaccgtc
     3001 accttctcgc ccaccgtgcc ctcgatccat acatggtcct cgacgaagag gagcttgcag
     3061 ccggctggga gcgcgatggt gctgtgcgca acctcgttct ggatgaggat gcggtctgtg
     3121 ctcccgtccg agctgttgat cgggacattg ctcaaggggg tcgcatgagt cacgcgccac
     3181 accgtgatcg tgccgtcggc attgaaccgg aggtggtatc ccctccagta cgccgagctg
     3241 ttgctcgcgc cattggaata ccgcggtagg tacgcgccgc ttgcgatcgc ggtcgccttc
     3301 agggtcccga agtccgccga gatggcggca aagtccactt gcgggaccgg gaagctccag
     3361 agcgtctggt tggggcccga cccccacacg cccgcgaccg tctgggtcgg ggagcagccg
     3421 taggaacccg tgcaggacca ggtggcgacc gagctcgtca ccggggcgtt cgcggtcccg
     3481 tccatgtgga tgccgccgtt ggagtggtac ggcccgttga tcacgcggtc cgcgcccgcc
     3541 cacacgctat cgttgaggac ataggaatag agcgcgaccg tgggccgcgt gtagcgcacg
     3601 gtgagcgtgc ggttgacggt gctcccgctt ggctgccccg tcgagtcgat gtcgatcgag
     3661 gtcacctgac cgcaggaggt gttcccctgg atgtcgaggg tataggtgcc ggccacgtcg
     3721 ctctccgggt cctcgtacgg gatggcgtag ggacctgggt tcccggttcc gttcgtgagg
     3781 tctcccggat tgtgcgcgag gaaccagcgg tagtactcga ggccggcttc cgcgatcgag
     3841 aacgccttcg cgcggtcggt gctcgcggtc tgcgccttgt tggtcgtgag cacgaagctc
     3901 gcgaggctcc cgagcaccat gaggaagatc gcgccgaaca cgatcccgag gaggaggagg
     3961 tacccgcgcg gaggacgctg catgccccta ttctactccg tcggcaggtt gcgcatcgtc
     4021 gcggtgcccg agagtgtgaa cacgttcggc gcccgggtcg ggttgaggtc tacgtcgagg
     4081 cggaccccga ccgagacgat ctccgcgaga tcgatgggcg acgtgagctc caggccgtcg
     4141 gcgccgtagt accggaagat gggcgtcgtg ttgcggaccg cgaccgcgat ggtctcgatc
     4201 gtctccggct gtcccgtata ggtcgcgggg ttcccggtcg agttcgtgat gccgcggtag
     4261 agggtgccgt ttgaaagata cgcccggatg cgctcgatgc cgccatcctt gtcgatgtcc
     4321 gcatagaacg tgatggtgtt gctctgcgcg tccgtaatga ggtaggaccc gtcgtccccg
     4381 taggaggcct cgcggatgtt ctgcagggtg aacgcatggc cgcggcgggc gctttcgagg
     4441 gccgccgtct gctcgaggat gtaggcgttg ctcctataga aatactggat ggagcccatg
     4501 agcgcgacgc ccgcggttac gacgatcgcg acgacgacca tcgtctcgac gagggtgaag
     4561 ccccgcatat tactcgaagg tcgccacata gaagatatgt cccgcgacgt tcacgttcgc
     4621 gaagttcgcg gtcgtgtagc cggctttcgc gatcgagacc gtataggcgg acgaggacgt
     4681 gaggttcccg aagtaggccg tgccgcagga ggtcgtctcc accgtctggc tgaacccggt
     4741 ccgcgacagc gtcacgtccg cgcccggcac caccgcgcct gtgttgtcct tcaccgtgac
     4801 gagcagcatg ttcgtcgtat tggcgccgag gtagaggctc gagtcggtcg cgctccccgg
     4861 cgcaagcgcg tacccggggg acgggcaggc ctcgacgacg tcgtagccgt tcagggtaag
     4921 ctcatacgca tcccactcaa gcgagagcgc cttcaccccc gaaccgttcg tcgagtccgc
     4981 aacggtcgtc ttgtagagcg ccgcgccggc gccggtcgag cccacggtct tcgcgccccg
     5041 taaggtgaac gcgatgttcg gcaacggcgt cgcgccgcgg cggtaggaga gcgaccaatc
     5101 gcgcagctcc ggcgtctctg cggaggcggt gctcgtgaga ttggcgcgga gcttcagggt
     5161 cgggtaggtc gaggtcgcga tgccggcaag cgagaccggg aaggtgtcga agcccgcctc
     5221 gttcccgggc aggaccgcgt tcgggagcac ggcgcccgag gcgtccacta cctggatcag
     5281 cgcctcggtg ctcgcggacg ggttcagggt cgcattgagc tcgccccatt catggagcgc
     5341 cgtcggcgtg acggcgaccg agaccgcgtg cccggagccc gggtacccca tgccgctatc
     5401 cacgagcgcg agcgcgccgg cggacacccc gacattcacc gcctccgcga tctgcgagag
     5461 gtccgcgaac gtatctgcga acgagagcgt ctgcggcgga ctgaaggtac gaagcgtgag
     5521 gtcggcgagg aggtcgatcg cgaaggtgcc ggtggtggtc tggttctcga ccacggtcag
     5581 gtaccccggg gtcgggttct ggttcgtcgc gtcccgcgcg taggtggtcg cggtcgagta
     5641 gccgctcttg gtcgtctcga tgcggtagtc ggtcgacggc agcgcgcccg gcaggtacac
     5701 gagcccctcg gcgttcgtga acgtcgtgag gtcgaccgca ggcaccgtgc tcgggttctc
     5761 gatgtggacc tcggcgcctg cgaccgggcc cccgagcgcg tccaccacga tgatctggag
     5821 cgt
//
LOCUS       DASPKG010000091         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_399753, whole genome shotgun sequence.
ACCESSION   DASPKG010000091 DASPKG010000000
VERSION     DASPKG010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399753"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..659
                     /locus_tag="VEA36_03680"
     CDS             237..659
                     /locus_tag="VEA36_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29434.1"
                     /translation="MDERTLEALKGSIAKWEAIVAGTGDEKGVNNCPLCQLFNGPSEE
                     ANLGCKGCPVADASGDTGCLNTPYEHWTEAPDLIKSPDGGYMIGGIGSRILAQAELNF
                     LRSLLPASAIEAEGGDPQGLRAEHESAAPEGGDAQGQS"
     gene            724..954
                     /locus_tag="VEA36_03685"
     CDS             724..954
                     /locus_tag="VEA36_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29435.1"
                     /translation="MSNDFGSDWDRLLKAQQAQEPPLIEVTIWGADGQVVHHSFERGF
                     RMTEEQHQEAIRLIEGGMRACDAARLVTGATG"
     gene            951..1139
                     /locus_tag="VEA36_03690"
     CDS             951..1139
                     /locus_tag="VEA36_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29436.1"
                     /translation="MSDIAEGLLSLAEDLLFEVDHRPLCRDERMKAEKTRIIVDQILA
                     IDDGMKMQRVEDAARKVA"
     gene            1139..1783
                     /locus_tag="VEA36_03695"
     CDS             1139..1783
                     /locus_tag="VEA36_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HYF29437.1"
                     /translation="MPTAKKGYYLASGERVPSVTTVLSRFKDAGGLIHWAWALGKEGK
                     DYREERDKAADAGTMAHAAVEAWIHGQPVSFEGDPEVCAKARTAFGAFLEWARQTHLQ
                     VTHTEVPLVSEKYKFGGTFDALLIQGKRSMGDWKSSNNCYPEYLVQVAAYGKLWEENN
                     PEQPIEGGYHLLRFDKTYGDFHHHWWGELDRAWDAFLHLRALYEIQKELTKRAA"
     gene            1783..2499
                     /locus_tag="VEA36_03700"
     CDS             1783..2499
                     /locus_tag="VEA36_03700"
                     /inference="COORDINATES: protein motif:HMM:NF016024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAD52 family DNA repair protein"
                     /protein_id="HYF29438.1"
                     /translation="MFTDAQKAALSAKLDGAHVKQRKQAGRQLSYIEGWWAIAEANRI
                     FGYDAWDRETVELRLLSERATKIGANKDRDGHAVAYMARVRVRVIVGERAIIREGCGY
                     GSGIDVDLGSAHESAIKEAETDAMKRGLMTFGNPFGLALYDKDQENVEHNGNGHKPAE
                     DSPDVARAKEMAHKIKAGIDKATSASDAALGLKVAGTGKDREDVAEIRKHLGDGWANR
                     LHELAAFKIAELTKPKDKAA"
     gene            2496..2753
                     /locus_tag="VEA36_03705"
     CDS             2496..2753
                     /locus_tag="VEA36_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015717502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29439.1"
                     /translation="MSDRYDVMSGRNYTDRNGDSKTAWTKVGTMFTTERGFRIALDAL
                     PLPQLRDGKLECTLVCFPPKPREEKGGNRGGDDLGGDSIPF"
     gene            2757..2966
                     /locus_tag="VEA36_03710"
     CDS             2757..2966
                     /locus_tag="VEA36_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29440.1"
                     /translation="MSLYVRRKEGETWRECVARYAGRHGLATECLDIFDQEVGKGVPG
                     GKAAWDALCEWDCLDFRDDDVNPFI"
BASE COUNT          630 a          970 c         1037 g          483 t
ORIGIN      
        1 ctgactgtcc tcgattacgt gtgccgggtg gggcgggcga tctcacgcaa gcctgagccg
       61 acgaccgcgc agaagctgct cgccgtgcat ttgcacttcg cggacccgcg caacacagga
      121 gcgatgaagt gatgggcgtt ccggcttcgc cgtcgcgtgc ttgcccttcg ggtcgagccg
      181 ccaaggggcg tctcgatcgc ttcgcgacta acgcccgcta acgcaaggag actgtgatgg
      241 acgagcgcac gctggaagcc ctcaagggca gcatcgcgaa gtgggaggcg attgtcgcgg
      301 ggactggcga cgagaaggga gtcaataact gcccgctctg tcagctgttc aacggcccca
      361 gcgaggaagc gaacctcggc tgcaagggct gccctgttgc agacgcctcc ggcgacacgg
      421 gttgcctcaa cacgccgtat gagcactgga ccgaggcgcc cgacctcatc aagtccccag
      481 acgggggcta catgatcggc ggcatcggct cacgcatcct cgcccaagcc gaactcaact
      541 tcctccgttc gctcctcccc gcgtcagcga tcgaagccga aggcggagac ccgcaggggc
      601 tccgtgcgga gcacgagagc gcggcccccg aagggggaga cgcccaaggt caatcgtgaa
      661 gctccgctac ctcgtgattg ccgccctctc ctacctcctc accggcctta tggggatacc
      721 gatatgagca acgatttcgg atctgattgg gaccgattgc tgaaagcaca gcaggcgcag
      781 gagcctccgc ttatcgaggt gacgatctgg ggtgcggatg gacaggtcgt tcaccactcc
      841 ttcgagcgcg gcttccggat gaccgaagag cagcaccagg aagcgatccg cctcatcgag
      901 ggtgggatgc gcgcttgcga tgcggcgcgc ctcgttacgg gagccacagg atgagcgata
      961 tcgccgaggg cttgctttcg ctcgccgagg atctgctgtt cgaggtggac catcgccccc
     1021 tctgccgtga cgagcgaatg aaggccgaga aaacccgcat catcgtggac cagatcctcg
     1081 ccatcgacga cggcatgaag atgcagcgcg tggaagacgc tgcccggaag gtggcgtgat
     1141 gccgaccgcg aagaagggct actacctcgc gagcggcgag cgcgtcccgt ccgtaacgac
     1201 ggtgttgtcc cgctttaaag atgccggcgg acttattcac tgggcctggg cgctcggcaa
     1261 ggaggggaag gactaccgcg aggagcgaga caaggccgcc gacgccggca cgatggccca
     1321 cgctgccgtc gaggcgtgga tacacggcca gccggtctcg ttcgagggcg atcccgaggt
     1381 gtgcgcgaag gcgaggaccg cgttcggcgc cttccttgag tgggcgcggc agacgcacct
     1441 tcaagtaacg cacaccgagg ttccgcttgt atccgagaaa tacaagttcg gcgggacgtt
     1501 cgacgccctg cttattcaag gcaagcggtc catgggtgac tggaagtcat ctaacaactg
     1561 ctacccggaa tacttggttc aagtcgccgc ttatggtaag ctctgggaag aaaataatcc
     1621 agagcagcct atcgagggcg ggtatcactt gctccgcttc gataagacct atggtgattt
     1681 tcatcatcac tggtggggtg agcttgatag ggcgtgggat gccttcctgc atcttcgcgc
     1741 gctgtacgaa atccagaagg aactgacgaa gagggccgcg taatgttcac cgacgcacag
     1801 aaggccgcgc tctcggcgaa gctcgacggc gcgcacgtca agcagcgcaa gcaggctggg
     1861 cgacagctct cgtacatcga aggctggtgg gccatcgccg aggctaaccg gatcttcggg
     1921 tacgacgcat gggaccgcga gacggtcgag cttcggctgc tctcggagcg cgccacgaag
     1981 atcggcgcaa acaaggaccg cgacggccac gctgttgcgt acatggcgcg ggtccgcgtg
     2041 cgcgtcatcg tcggcgagcg ggccatcatc cgcgagggct gcggctacgg cagcggcatc
     2101 gacgtcgatc tcggctcggc gcacgagagc gccatcaaag aggctgagac cgacgcgatg
     2161 aagcgcgggc tgatgacctt cggcaacccc ttcggcctcg ccctgtacga caaggaccag
     2221 gagaacgtcg agcacaacgg caacggccac aagcccgccg aggactctcc cgacgtcgcc
     2281 cgcgccaagg agatggcgca caagatcaag gccggtatcg acaaggctac gagcgccagt
     2341 gacgccgcgc ttggcctgaa ggtcgccggc accggcaagg accgcgagga cgtcgccgag
     2401 atacgcaagc acctgggcga cggctgggcg aaccgcctgc acgaactcgc cgcattcaag
     2461 atcgcagaac tcaccaagcc gaaggacaaa gcagcatgag cgacagatac gacgtgatga
     2521 gcggccggaa ctacaccgac cgcaacggcg acagcaagac cgcctggacg aaggtgggga
     2581 ccatgttcac gaccgagcgc ggcttccgca tcgccctcga tgccctgccc ctcccgcagc
     2641 tccgcgacgg caagctcgaa tgcacgctgg tctgcttccc gccgaagccg cgcgaagaga
     2701 agggcggcaa ccgcggaggc gacgacctcg gcggggactc gattccgttt taggccatgt
     2761 cgctctacgt cagacgcaag gagggcgaga cgtggcggga gtgcgtggcg cgctatgccg
     2821 gccggcacgg gctcgccacg gaatgcctcg acatcttcga ccaggaagtc ggcaagggcg
     2881 tccccggagg caaggccgca tgggacgcgc tgtgcgagtg ggactgcctc gacttccgag
     2941 acgacgacgt gaacccattc atatgaagaa ggcgcgcgcc aagctcttcg actccccgca
     3001 cttcatggtg aggagggaga aggtcgagcg gaagccggat gcgtggttcc ggtcgagcgc
     3061 gcaccttgcc tacgtcagaa cgctccggtg cgtgtgctgc ggctccgaca gggagattgt
//
LOCUS       DASPKG010000092         3552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_401946, whole genome shotgun sequence.
ACCESSION   DASPKG010000092 DASPKG010000000
VERSION     DASPKG010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401946"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VEA36_03715"
     CDS             complement(<1..322)
                     /locus_tag="VEA36_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HYF29441.1"
                     /translation="MIITTLFALGSVLLVGAISLIGIFTLSLRESLIKSVTFVLVGVA
                     TGALLGDAFIHLIPEAFAETTDTVALSLTIIAGMLVFFVLEKYLHWHHHQGLESAEPE
                     VHPVG"
     gene            402..821
                     /locus_tag="VEA36_03720"
     CDS             402..821
                     /locus_tag="VEA36_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HYF29442.1"
                     /translation="MERISTVAADLRHARLRATRQRTSLLTVLRQATAPLGVEALVRQ
                     GRGAFDTATAYRMLEAFVAAGLARQMAFSQDRALYEAVGTHHHHATCRSCGKIVDIEA
                     CLPRTLNERVRNAAGFASIDDHALEFFGICTSCAQKD"
     gene            794..1912
                     /locus_tag="VEA36_03725"
     CDS             794..1912
                     /locus_tag="VEA36_03725"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HYF29443.1"
                     /translation="MHILRAKGLALTLATFVLAVIPTAAVAQSLQPDSETFAKARVIS
                     VAQAGTQQVPGTELTADVQTLTAEILEGPEQGKVVTFVNDFAQLEEGDVFYLRHLASP
                     LEGTEFYSVADPYRLPIIFGLVAVFLALLVLFGGIQGLRGLVSLAGSFILIFYLLLPG
                     IVAGYPPVLVAVGVSSLIIIAGSYITHGFNRTTTAAVLGMIATVAASSLAAWYVVGAA
                     ALSGFSGEESGYLHFQYGGTLDLVGILMSGIIIGLLGVLYDVAIGQAVAVEELMAAGQ
                     HLDKQTIYQRAIRIGREHIGALVNTLAIAYVGVALPLLLLLGNATAPLGYILNGEVIA
                     TELVRILIGASGIILAVPVTTLIAVFLVRKTSTVRHTH"
     gene            1978..2757
                     /locus_tag="VEA36_03730"
     CDS             1978..2757
                     /locus_tag="VEA36_03730"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HYF29444.1"
                     /translation="MNDRRMRIALLAVLVLLALFLLAKTWDAAFGRGPNEPVNVITVT
                     GTGEAVVVPDIAQIDFTVTEQAATVAEAQAKTTERTNSALEAVKQLGIEEQDVKTTYY
                     AINPRYEYANTVCMAGMPCPPPGSPRIIGYEASQAIQVKVRDTAKAGQVLETLGSLGV
                     QNISSPNFTVDNEDEVRAQAREEAIKEAREKAKVLAKQLGVRLGKTVSFYENEGPYPY
                     YGYGKGGGAMESDIRVSAVAPTLPTGQNETSVSISITYEIW"
     gene            2878..3063
                     /gene="secE"
                     /locus_tag="VEA36_03735"
     CDS             2878..3063
                     /gene="secE"
                     /locus_tag="VEA36_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HYF29445.1"
                     /translation="MNSFFTYLKNVRGELSHVVWPGRSQAILHTVLIVVISALVALLL
                     AGFDYVFTGVVNQFISY"
     gene            3087..>3552
                     /gene="nusG"
                     /locus_tag="VEA36_03740"
     CDS             3087..>3552
                     /gene="nusG"
                     /locus_tag="VEA36_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HYF29446.1"
                     /translation="MDMKSAPQFSDEGLSTEPRWYTIHTYAGYENAVERNLRQRIESL
                     GMENKIFEVVVPTEKKIRVKGGKRIVEEEKIYPGYILVRMIVDEDSWFVVRNTPRVTG
                     FVGSGTTPVPMEDAEVTSLMKRMGQEAPKHRIDLMKDDPVIIADGPFKDLEGK"
BASE COUNT          677 a         1105 c         1122 g          648 t
ORIGIN      
        1 tcccgaccgg gtgcacctcc ggttccgcgc tctcgagccc ctggtggtgg tgccagtgga
       61 ggtatttttc aaggacgaag aagacgagca tgcccgcgat gatggtcagc gagagtgcga
      121 cggtgtccgt cgtttccgcg aaggcctcgg ggatgaggtg gatgaaggcg tcgccgagca
      181 aggcgccggt tgcgacgccg acgagcacga acgtcacgct cttgatgagg ctctctctga
      241 gcgagagcgt aaagatgccg atgagggaga tggcgccgac gagcagaacg ctgccgagcg
      301 cgaacagggt agtaatgatc atgcgggaag catagcgtag gcacccgata cttgcaaatc
      361 gttcgcatgt ggaaatcagg cctatgcggt atcatgcaga tatggaacga atctcaacgg
      421 ttgcggccga cttgcgccat gccaggctgc gcgccacgcg tcaacgcacg agcctgctta
      481 cggtgctgag acaggcaacg gccccacttg gggttgaggc actcgtgcga cagggacggg
      541 gcgcgttcga cacggccacc gcgtatcgca tgctcgaggc gttcgttgcc gcgggcctcg
      601 cacggcagat ggcgttctcg caagatcgcg ccctttatga agcggtgggg acgcatcatc
      661 atcacgcaac atgtcgttcc tgcggcaaaa tcgtggatat cgaagcctgc cttccgcgca
      721 cactcaatga gcgggtgcgc aatgcggcag gattcgcgag catcgacgac cacgcgttag
      781 aattcttcgg catatgcaca tcctgcgcgc aaaaggacta gcgcttacac tcgccacgtt
      841 cgtgcttgcg gtaataccga cggcggcagt ggcgcagagc ctgcagcccg atagtgagac
      901 cttcgcgaag gctcgcgtca tatcggtcgc gcaggcgggc acgcagcagg tgccgggtac
      961 ggaactcacc gcggacgtgc agacgctgac cgctgagatc ctcgagggac ccgagcaggg
     1021 gaaggtggtc accttcgtaa acgacttcgc gcagcttgag gaaggggatg tcttctacct
     1081 ccggcacctc gcaagccccc tggaagggac cgaattctat tcagtcgccg atccctaccg
     1141 cctaccgatc atattcgggc tcgtcgccgt gttccttgcg ctgctcgtac tgttcggcgg
     1201 gatccagggt ctgcgggggc tggtgtccct tgccgggagc ttcatcctta tcttctacct
     1261 gctcctcccc ggcatcgtcg ccgggtaccc gccggtcctg gtcgcggtcg gggtctcgtc
     1321 gctcatcatc atcgcgggct cctacatcac ccatgggttc aaccgcacga cgaccgcggc
     1381 ggttctcggc atgatcgcga ccgttgccgc aagcagcctt gccgcctggt acgtggtcgg
     1441 ggccgcggcg ctttccggat tctcggggga ggagagcggc tacctgcact tccaatacgg
     1501 cggaacgctc gacctcgtcg gcatcctcat gagcgggatc atcatcggtc tcctgggcgt
     1561 cctctacgac gtcgcgatcg ggcaggcggt cgcggtcgag gagctcatgg ctgcggggca
     1621 gcatttggat aagcagacga tctaccagcg ggccatccgc atcggacgcg agcacatcgg
     1681 cgccctcgtg aatacgctcg cgatcgccta tgtcggcgtc gcgctcccgc tgctcctcct
     1741 gctggggaac gcgacggcgc cgcttggcta catcctcaac ggggaggtga tcgcgaccga
     1801 gctggtacgc atcttgatcg gcgcctcagg catcatcctt gcggtcccgg tgacgaccct
     1861 catcgcggta ttcctcgtca gaaagacctc taccgtacgt catacccact aagcgcgtgc
     1921 ccgcctcggg tatactcgct gtataaccct taatgacgta cgtacatgag tgaactaatg
     1981 aatgatcgac ggatgcggat cgcgctcctt gccgtgctcg tgctgctcgc gctcttcctg
     2041 ctcgcgaaga catgggatgc ggccttcggg cgcggcccga atgagccggt caacgtgatc
     2101 accgtcaccg ggaccgggga agcggtcgtg gtgcccgaca tcgcgcagat cgacttcacg
     2161 gtcaccgagc aggcggcaac cgtcgccgag gcgcaggcca agacgaccga gcggaccaac
     2221 tctgcgctcg aggccgtgaa gcagctcggg atcgaggagc aggacgtgaa gacgacctac
     2281 tatgcgatca acccgcgcta cgaatacgcc aatacggtct gcatggccgg catgccctgc
     2341 ccgccgcccg gctcgccccg catcatcggg tacgaggcgt cccaggcgat ccaggtgaag
     2401 gtgcgcgata ccgccaaggc aggccaggtg ctcgagaccc tcggttcgct cggggtgcag
     2461 aacatctcga gcccgaactt cacggttgat aacgaggacg aggtgcgggc gcaggcgcgc
     2521 gaagaggcca tcaaggaggc tcgcgagaag gcgaaggtgc tcgccaagca gctcggggtg
     2581 cgcctcggaa agaccgtctc cttctacgag aacgaaggtc cgtacccgta ctacggatac
     2641 gggaagggcg gtggcgcgat ggagtccgat atccgggtat ccgcggtcgc accgacgctt
     2701 ccgaccgggc agaacgagac ctcggtctcc atctcgatca cgtacgagat ctggtaatcg
     2761 ctcgtttccc gtccacaggc cccgcccccg cggggcctgt gccgtttgac agcgaaaaaa
     2821 gccagtagta tccttgcttc atacaaggcc cgcggggctt ttttatttga gccacatatg
     2881 aactcattct tcacctacct caagaacgtc cggggagagc tctcccatgt ggtgtggccc
     2941 ggccgcagcc aggccatcct ccataccgtg ctcatcgtgg tgatctctgc cctggtcgcg
     3001 ctccttctgg ccggcttcga ttacgtcttc acgggcgtcg tgaaccagtt catttcttac
     3061 taagcttccc taagtacact tggcgtatgg acatgaaatc agcaccgcag ttcagcgatg
     3121 aaggactctc gaccgagccg cgctggtaca ccatccatac ctacgcgggg tacgagaacg
     3181 cggtggagcg caacctccgc cagcgcatcg agtcgctcgg catggagaac aagatcttcg
     3241 aggtggtcgt cccgaccgag aagaagatcc gcgtgaaggg cggcaagcgc atcgtcgagg
     3301 aggagaagat ctatccgggc tacatcctcg tccggatgat cgtggacgag gactcctggt
     3361 tcgtggtccg caacacgccg agggtgacgg gcttcgtggg gagcggcacc acgcccgtgc
     3421 cgatggaaga cgccgaggtt acctcgctca tgaagcgcat gggccaggaa gcgcccaagc
     3481 accgcatcga cctcatgaag gacgatccgg tcatcatcgc cgatggcccc ttcaaggacc
     3541 tcgaggggaa gg
//
LOCUS       DASPKG010000093         4567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_671281, whole genome shotgun sequence.
ACCESSION   DASPKG010000093 DASPKG010000000
VERSION     DASPKG010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671281"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VEA36_03745"
     CDS             complement(<1..1135)
                     /locus_tag="VEA36_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HYF29447.1"
                     /translation="MQTLIDRLHGLLRRSERYTKTDMVYAVSGLFWTTSGQAVAILCA
                     IPLAVVLANLLTPVEYGIYRYVLTVAAVIGAFSLTGLPAVLTRAAARGFDGSLKEAFW
                     LTIKGSGLVILGSFAGAAYYYLQGNSILAGGMALIGIFWPWLQSATLFSAFLSGKLLV
                     RMNTAYGNRRTVIHTLVMLATAYLYPEPLAILLSYFVIQTATALYYYHKTRKLHVEND
                     ERDPELKFLGKHMSFLNIINVLIDKLDALLLFQFVGAAELAIYAFAEMVPDVLNRFTK
                     NVSALALPKFVTRGDAAGIWRKTFLISLLLLPVALLYCALAPFLFPIFLPAYTAAVPF
                     TQALALLAVINAALPSTYIDAQGAIKTRYVITFVTNITKVIAVG"
     gene            1172..3346
                     /locus_tag="VEA36_03750"
     CDS             1172..3346
                     /locus_tag="VEA36_03750"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HYF29448.1"
                     /translation="MAKVTKYIVLGALFLVPFLPLYVANAQFFPFITGKGFLFRILVE
                     IAFVGWVALAAMDKRYRPQFSWLLAIFGAFTLWIFIADLFAVNPHKALWSNFERMDGF
                     VTLIHVFLFFAVMGAMLSVEKLWRKWWLVFLAVAGIVTLIGVMQMLCAGQACGAPGQF
                     FAINQGGVRVDGRIGNAIYLAVYLMFAAFVAIWVAIESKGWLRYGLIVLSAFAVFILF
                     NTATRGAIVGLVAGSALSALLFLVTSGGRGRKIAGGLLVALLILIGGFLLVKDNPAVA
                     EHPVWGRIASISLKELEVRFTLWSMAAEGLTERPLTGYGQEGYNYIFNGYYRPALYTQ
                     EPWFDRAHSAYMDWLVAGGIPAFLLFVALLIAAFVLVLRTPNLSTATRIMLAGLIAAY
                     AVQALVVFDNLLSYLPLAAVLAYLHSRAMRPIDALEKLPELPRGSAEAIVIPVAGAAI
                     LFLILTVNVPNMRGANHLVYAISSSSGGPAANLERFRAALATHTFATQEVREQFVNFT
                     VSLIGQPGVPDQLKNDFAMLAITEIEKEVERAPADARVRMQAALLHRAVGNYPQALAH
                     LAAARAASPNRQLIMLEQGVLLAEVGEQEAARAVLEEAYALLPGNKDLAARVAAGLIT
                     AGADLRAEEILMEAHGARIVDHDVLVNAYAITKQYGKLIETVMLQVKNRPDAPEARYR
                     LAAAYALAGEYQKARVEAQTAMAKFPATVAQGQSFIASLPTQ"
     gene            3343..4380
                     /gene="murB"
                     /locus_tag="VEA36_03755"
     CDS             3343..4380
                     /gene="murB"
                     /locus_tag="VEA36_03755"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HYF29449.1"
                     /translation="MTIEESVPLATLTTLRVGGPARYVISIKKPADLPEAVAFAAERS
                     LPITVLGGGSNVLAPDAGYAGVVLHMQLPGISFADAGAAVLVTAGAGASWDVLVEKAV
                     ARGLWGIENLAGIPGTVGAAPVQNIGAYGMELEQVFVSADVYDRRTGEVRPYMHNECA
                     FEYRQSRFKSEPELIITSVTLELKKDGSPQLQYGDLAARAAVGEPLGTPAAVAAAVRS
                     IRARKFPDLAVCGTAGSFFKNPIISEEKYTKLAVRFGDITEIHGAIPRYPLQREGEVK
                     IPLAYILDKVLGMRGYRIGPVHLFNAQPLVLVADEGATQQDIDALANDVAQKVFEATG
                     IAIEREVRNIA"
BASE COUNT          841 a         1445 c         1461 g          820 t
ORIGIN      
        1 cgcccaccgc gatcaccttc gtgatattcg tgacgaaggt gatgacatag cgggtcttga
       61 tggcgccctg tgcatcgata taggtagaag gaagcgccgc attgataacc gcaaggagcg
      121 ccagcgcctg cgtgaacggc acggccgcgg tgtaggccgg caggaagatc gggaagagga
      181 agggtgcgag agcgcaatac aggagtgcga ccggcagcag gaggagtgag atcaggaagg
      241 tctttcgcca aatgcccgcc gcatcccccc gcgtcacgaa cttcgggagc gcgagcgccg
      301 agacattctt cgtgaatcta ttcagtacat caggcaccat ctccgcgaat gcatagatgg
      361 cgagctcggc cgcgcctacg aactggaaca agaggagcgc atcgagctta tcgatgagca
      421 cgttgatgat gttcaggaag ctcatgtgct tcccgaggaa cttcagctcc ggatcccgct
      481 catcgttctc gacgtggagc ttccgtgtct tgtggtagta gtagagagcg gtcgcggtct
      541 ggatgacgaa gtaggaaagg agaatcgcga gcggctcagg atagaggtaa gcggtcgcga
      601 gcatgacgag tgtatggatt accgtccggc gattgccata ggccgtattc atccggacga
      661 ggagcttccc cgagaggaag gcggaaaaga gggtggcgct ctgcagccac ggccagaaga
      721 tgccgatgag cgccatcccg cccgcgagga tggagttccc ctggagatag tagtaggcag
      781 cgccggcgaa ggaaccgagg atgacgagcc cgcttccttt gatcgtcagc cagaacgctt
      841 ccttgagcga cccatcgaag ccgcgggcag cagcgcgggt gaggaccgcg ggaagcccgg
      901 tcaacgagaa tgcgccgatg actgccgcca ccgtgagcac gtaccgatag atgccgtact
      961 cgacgggggt gaggaggttc gcgagcacca cggcaagcgg gatcgcgcag aggatcgcga
     1021 ccgcctgccc ggaagtagtc cagaaaaggc ccgagacggc gtacaccata tcggtcttgg
     1081 tgtagcgctc gctccggcgt aagaggccgt gaaggcggtc aataagggtc tgcatgggag
     1141 cggggcgtca gtactatata ctaccaacat cgtggccaag gttaccaagt acatcgtgct
     1201 cggggcgctc ttcctcgtcc ccttcctccc gctctacgtc gcgaacgcgc agttcttccc
     1261 cttcattacg gggaagggat tcctcttcag gatcctggtg gagatcgcct tcgtggggtg
     1321 ggtggcgctc gccgcgatgg acaaacgcta ccgcccgcag ttctcctggc tcctcgcgat
     1381 cttcggtgcc ttcacgctct ggatattcat cgcggacctc ttcgccgtga acccgcacaa
     1441 ggcgctatgg agcaacttcg agcgcatgga cgggttcgtg accctcatcc acgtcttcct
     1501 cttcttcgcg gtgatgggcg cgatgctctc cgtggagaag ctctggcgga agtggtggct
     1561 cgtcttcctt gcggtcgcgg gcatcgtgac gctgatcggc gtcatgcaga tgctctgcgc
     1621 ggggcaggcc tgcggcgcgc ccggccagtt cttcgccatc aaccagggcg gggtgcgcgt
     1681 cgatggccgc atcggcaacg cgatctacct cgccgtctac ctcatgttcg cggcattcgt
     1741 cgcgatctgg gtcgcgatcg aatcgaaggg gtggctgcgg tacggtctca tcgtcctctc
     1801 cgcgttcgcc gtcttcatcc tcttcaacac ggcgacgcgc ggcgcgatcg tggggcttgt
     1861 cgccggcagc gcgctttcgg cgctcctctt cctcgtcacg tcaggcggcc gcggcaggaa
     1921 gatcgcgggc gggctcctcg tcgcgctcct catcctcatc ggcgggttcc tcctcgtgaa
     1981 ggacaatccg gcggtcgccg agcacccggt gtgggggcgc atcgcctcca tctcgctcaa
     2041 ggagctcgag gtgcgcttca cgctctggag catggcggcc gaggggctca cggagcgtcc
     2101 gctgaccggt tacggccagg agggctacaa ctacatcttc aacggttact accgtcccgc
     2161 gctctatacg caggagccct ggttcgatcg ggcgcatagc gcgtacatgg attggctggt
     2221 cgcgggcggc atcccggcat tcctcctgtt cgtcgcgctc cttatcgccg cgttcgtcct
     2281 cgtgctgcgc acccccaatc tctcgaccgc gacccggatc atgctcgcgg ggctgattgc
     2341 cgcctacgcg gtccaggcgc tcgtcgtgtt cgataacctg ctttcgtacc tcccgcttgc
     2401 cgcggtcctc gcctatctgc atagccgcgc gatgcgcccg atcgatgcgc tcgagaagct
     2461 gccggaactc ccaagaggga gcgcagaggc gatcgtcatc ccggtcgcgg gcgccgccat
     2521 cctcttcctc atcctcacgg tgaacgtccc gaacatgcgc ggcgcgaacc acctcgtcta
     2581 cgcgatctcg tcctcgtcag gcggtcccgc cgcgaacctc gaacgattcc gcgcggcgct
     2641 tgcgacccat acctttgcca cccaggaggt gcgcgagcaa ttcgtgaact tcacggtgag
     2701 cctgatcggg cagccgggcg tcccggatca gctcaagaac gacttcgcga tgctcgcgat
     2761 caccgagata gagaaagagg tcgaacgggc accggccgat gcccgagtgc gtatgcaggc
     2821 ggcgctcctc catcgcgcgg tcggcaacta cccgcaggcg cttgcgcacc ttgcggcggc
     2881 tcgtgccgcc tccccgaacc gccagctcat catgctggag cagggcgtac tgctcgctga
     2941 agtgggagag caggaagctg ctcgcgcggt cctcgaggag gcgtacgcgc tcctgcccgg
     3001 caataaggac ctcgccgcgc gtgttgcggc cgggctcata accgcgggcg ccgacctgcg
     3061 cgccgaggag atcctcatgg aggcgcacgg cgcccgcatt gtggaccacg acgtcctcgt
     3121 gaacgcctac gccatcacga agcagtacgg gaagctcatc gaaaccgtga tgctgcaggt
     3181 caagaatcgc ccggatgcgc cggaggcccg ctaccgactc gccgccgcct acgcgctcgc
     3241 gggcgaatat cagaaggcgc gcgtcgaggc gcagaccgca atggcgaagt tcccggcgac
     3301 cgtcgcgcag ggccagtcgt tcatcgcgag cctcccgacg caatgacgat cgaggaatcg
     3361 gtaccgctcg ccacgctcac cacgctccgg gtcggcgggc ccgcgcgcta cgtcatttcc
     3421 ataaagaagc ccgccgatct tccggaagcc gtcgcgttcg ccgccgagcg ttcgctcccg
     3481 atcacggtgc ttggcggcgg cagtaacgtg ctcgcaccgg acgcggggta cgcgggcgtc
     3541 gttctccaca tgcagctgcc cggcatctcg tttgcagatg cgggagcagc cgtcctcgtc
     3601 accgcaggcg ccggtgcctc ctgggacgtc cttgtcgaga aggcggtcgc gcgcgggctc
     3661 tggggcatcg agaacctcgc gggtatcccg gggacggtcg gcgccgcacc tgtgcagaac
     3721 atcggcgcgt acggcatgga gctcgagcag gtgttcgtgt ctgccgacgt atatgaccga
     3781 cggaccggcg aggtgcgccc gtacatgcat aacgagtgcg ccttcgagta ccggcagagc
     3841 cgcttcaaga gcgagccgga gctcatcatc acgagcgtga cgctcgaact caagaaggac
     3901 ggcagcccgc agctccagta cggggatctc gccgcccgcg ccgcggtcgg cgagccgctt
     3961 ggcaccccgg ctgcggtcgc ggctgcggtt cgttcgatac gcgcgcgcaa gttcccggat
     4021 ctcgcggtct gcggcacggc cggctccttc ttcaagaacc ccatcatctc cgaggagaag
     4081 tacacgaagc tcgcggtgcg gttcggggac atcacggaga tacacggcgc gatcccgcgc
     4141 tatcccttgc agcgcgaagg cgaggtaaag ataccgctcg cgtacatcct ggataaggtg
     4201 ctcgggatgc gcggctaccg catcgggccg gtgcatctct tcaacgcgca gccgctcgtc
     4261 ttggtcgccg atgagggcgc gacgcagcag gacatcgatg cactcgcgaa tgatgtcgcg
     4321 caaaaagttt tcgaggcgac cggcatcgcg atcgaacgcg aagtgcgcaa catcgcatga
     4381 agatttgata aaaatatttt cgaaagttat ccacacatgc atgcatgaca cgcacaaatt
     4441 ttttctcgac gcgcaataat aaatagcgga acacacgtgc aagaaaatct ttcttgcgtg
     4501 tgggtcgaca gatactagtc atagcaaatt ttcattcaga cctatggcag caaagaagag
     4561 gaaggca
//
LOCUS       DASPKG010000094        10032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1483480, whole genome shotgun sequence.
ACCESSION   DASPKG010000094 DASPKG010000000
VERSION     DASPKG010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10032)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10032
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483480"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VEA36_03760"
     CDS             <1..780
                     /locus_tag="VEA36_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HYF29450.1"
                     /translation="GKRAETKDYFSLFLAELALALDGKWLSCSIEARMPVADRYTGTP
                     PASAYQFANDLPAIGRACDAVRVMTYDQQTADQKLNALRAGAPYFPVADAKWTEKVIN
                     LMSQDIPKSKLSIGVATYGRELEVTVSPTGKYTYKNLWSFNPGYAAQIEKKFKVKRSR
                     NAAGEMSLTYIDKKQKGGPTQKQLLALAPAGTPTGEQVAAGAKAYAAKNNTPVTFRFL
                     SWSDAGALQGHVALARALGVNGIAVFKFDGSEDKGIWTHMK"
     gene            862..2112
                     /locus_tag="VEA36_03765"
     CDS             862..2112
                     /locus_tag="VEA36_03765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYF29451.1"
                     /translation="MNTRPRWWETVTLPTYPKLRENLRTEVCIVGGGLAGLMSAYLLA
                     KAGRHVVLLEKKGIAWGATGATTAFLEQLIDTDVKDQIEMIGKEHTKHVWQSHGAAID
                     LIERIANDEGIECEFTRCPAYVFGTSRGEARDLKDEFAAMKGLGFPVFEYTKKLPFDQ
                     RYAFEIRNQAKYHPLKFINGLLPKLADMGVKLYEKSEVTKITGKGPFKVHTTGFLVRA
                     DATVTTTYQPFNNPKEVFLKKGMYKTYLVEFRVPKGKYPVAVYEDADNPYHYFRIDRN
                     GDHDLLLLGGEDHRIEIKMKEEKAYASLEEYAEEIFGTRYPVTRRWSGPILEPIDGFA
                     YIGRYAPGQYVATAFSGNGMTYSAISAMIFRDLITGKKNPYAHLYEPSRLPSMTHLWI
                     KGRDYTEELFRGALTDIFKNPKKR"
     gene            2115..2486
                     /locus_tag="VEA36_03770"
     CDS             2115..2486
                     /locus_tag="VEA36_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HYF29452.1"
                     /translation="MKTTIWSKYGYLWITLVLFLGSLIGHWVFAWYAFVDEQLAHGQA
                     IELNAYFIEVMRDTLENWQSEFLQLMWQVAGLAYLFYIGSPQSKEGDDRLEEKVDRIL
                     LAVDKDGEKTLKQIDKRHMRD"
     gene            complement(2483..3364)
                     /locus_tag="VEA36_03775"
     CDS             complement(2483..3364)
                     /locus_tag="VEA36_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HYF29453.1"
                     /translation="MTLHPSHFIALATACGVLILALGFGIYRYAALDRDADAYAAANL
                     EAHAAIKDLEERLGEAQAERLRLADALAAERAKTGLFERQIGELSGAVGTLEKLATTD
                     PELLAKYSKVYFLNENYSPSELALIDKSHLIDVTRPLEIHARVKPKLEALIDDAADDG
                     IELRIQSAYRSFGTQAALKSGYAVAYGAGANRFSADQGYSEHQLGTTVDFTTPALGSA
                     FTGFGRTEAYAWLTKNAHRYGFTLSYPEGNNYYIHEPWHWRYVGRELATKLHDEGKAF
                     YDLDQREINTYLASLFD"
     gene            3454..3741
                     /locus_tag="VEA36_03780"
     CDS             3454..3741
                     /locus_tag="VEA36_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYF29454.1"
                     /translation="MQAPAAATTAESRLPLRKKQGDRRVGIHVGKRIRGRRRLLGLSQ
                     RHVAARCGVTFQQIQKYECAASAINATMLWKLAQALDVPVHYFFDGLSDED"
     gene            3836..4243
                     /locus_tag="VEA36_03785"
     CDS             3836..4243
                     /locus_tag="VEA36_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HYF29455.1"
                     /translation="MKKGKFVPGAFDLRVRRSSAGLGLFTESAIPKGACIIEYVGKVI
                     TQKEADTSKSKYLFEISKNKTIDGKPKWNKAGYINHACKPNAESEIHNGRVFILATRN
                     ITPGEELTYDYGTEYFDEHIKPFGCRCVSCRKK"
     gene            complement(4250..4822)
                     /locus_tag="VEA36_03790"
     CDS             complement(4250..4822)
                     /locus_tag="VEA36_03790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYF29456.1"
                     /translation="MKLSLYYPVYPHIVTDAWGVEDPRYERFGFTHHNGIDIAAPAGE
                     AIHAPYAARVRQVSTGEDPRDLHITLLAPHAYEFPDTKCHVEITFMHVLLAIVLPGEQ
                     VAAGELIGFAGGNPLADYIHMAPKRVRAEGRQRFVDVDLNHASGTFDPLPYWNERYAQ
                     DLDSDGAKTLARLQRTLTNIERSLAEDVLS"
     gene            complement(4845..6140)
                     /locus_tag="VEA36_03795"
     CDS             complement(4845..6140)
                     /locus_tag="VEA36_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HYF29457.1"
                     /translation="MVHHDVLHTIKRAKQHESFIYPYYGAYSIAEIPPSVRLWLGLPP
                     DDRAALPFALRYNHAYRQVLFFFVDGLGFDHMVDYESVLPFFHRLKTRGDIYPLTTVF
                     PSTTPAALTTLHTNKTPQEHGLPEWTVYFEEFDRIIEPLPFRAHLSHEREGLSALGAD
                     PESLYEGRTFYEELADDGVCSYVYIKEEIAVSSYSAVSQRGSASRPFRDLDDLMQTLT
                     ATITEEAGPAYHFLYWDRVDSIEHVYGPRSKEHISALQELSGFLEAFTAKLSQEGVRD
                     TLFVLSSDHGQATIRNEDIIYLNDYVDLEANYTYGPNGKAIYPTGAPHDVFLHIYEGK
                     RAATINYLKRQLEGKAEVITTNEAIDRGLYGLGTPSARFMRRVGDVLILPYEGYHVWY
                     KHAPDVFFGQLGIHGGLSQREMIVPLAIAPLADLLHEVS"
     gene            complement(6143..7231)
                     /locus_tag="VEA36_03800"
     CDS             complement(6143..7231)
                     /locus_tag="VEA36_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HYF29458.1"
                     /translation="MPKFIPGLALTRDFYFDVVRPLIEQHCPGLQYSAGLVGHGSDVL
                     GFDTPTSTDHDWGPYLHIFFADLDFIVEKNRVDALLRKHLPTSYKGFPTHFTEGDYYL
                     KHQPKAKKRGPVNHIFRFWTIRSFFQHYLGFDISKQPSFRDWLLFPQQALIEVTGGQL
                     YRDDLDVEKYRRQFAWYPDDIWKYLLHIQWWKIADEIQSPSRSGEEGDEVGSMVVTAR
                     TVQKIMFMCFLMERRYAPYSKWFGTAFQRWLRSGPELYPLLLQILHEKRWLPRQKLLT
                     KAYRRLGEMHNALNLTPPVYPEETDFHGRGYPVLQLSGFFDGLKESIKNKKLKNMHYP
                     LGAIDQFIDHAHINHMDYVYKELGDIIR"
     gene            7320..7451
                     /locus_tag="VEA36_03805"
     CDS             7320..7451
                     /locus_tag="VEA36_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29459.1"
                     /translation="MKRSAIEVKQAKEASARKGTAGRRDHKQGAPSSNKGTGGKLAM"
     gene            complement(7508..7696)
                     /locus_tag="VEA36_03810"
     CDS             complement(7508..7696)
                     /locus_tag="VEA36_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HYF29460.1"
                     /translation="MASNNNGKSRRGFAAMDEEKQREIASKGGKAAHQKGTAHEFTSE
                     EAREAGRKGGQASRGGNR"
     gene            complement(7764..8339)
                     /locus_tag="VEA36_03815"
     CDS             complement(7764..8339)
                     /locus_tag="VEA36_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HYF29461.1"
                     /translation="MEKTRQKQAIRTKKRAIVTDPARTIEYFGVVNYATNERVIEEMR
                     ELVIAAPETPITLQVTSAGGPTGTAMSFYDTMRYILKPRLTTIGSGDVDSSGIIIFLA
                     GDTRYVTPHTTLLLHVAGRHFSEGKRYTADEMDAMLREDRLKDFQYASILAERSAGRL
                     DTDQVLRMMEKNTILTPLELVAYGLADRVLE"
     gene            complement(8447..8908)
                     /locus_tag="VEA36_03820"
     CDS             complement(8447..8908)
                     /locus_tag="VEA36_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HYF29462.1"
                     /translation="MTANDVLMQDHRELEDAYTDFVAAEGEEREELAKQILTDLTVHA
                     IVEEEFYYPLLEEAGEKALADEARAEHEGVKVYIAKLSMMDVDSEEYAPAMKAMMESV
                     LHHVAEEESTAMPRMEEIIGTERLEEMVPEMEARKEELRESTMKRLFAAIT"
     gene            complement(9047..9655)
                     /locus_tag="VEA36_03825"
     CDS             complement(9047..9655)
                     /locus_tag="VEA36_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEPxxWA-CTERM sorting domain-containing protein"
                     /protein_id="HYF29463.1"
                     /translation="MKISLAAAAAALALAASPASAVEYFHVTLTGFADNAIPSFVPSD
                     SNAFTFDLVFDPAVNRTTDDDNDTVAVSVTDNPFVSAELDITGIGTFDFTSSAQALGL
                     FRPDGILTFTTSCSGCAFTISALQPSLDSLDQVLGPVAATGGGNLIVGDDIDVNFRVT
                     GYHGRPFVATAPVPEPATWGLMILGFGFTGAALRARRRLAYA"
     gene            complement(9810..>10032)
                     /locus_tag="VEA36_03830"
     CDS             complement(9810..>10032)
                     /locus_tag="VEA36_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEPxxWA-CTERM sorting domain-containing protein"
                     /protein_id="HYF29464.1"
                     /translation="QASIADATSYFNQFAVTNFSVTYLLDGNVVGSGNLEILGAGAVP
                     EPATWAMMILGFGAVGSLVRRRRHGLAAA"
BASE COUNT         1897 a         3089 c         3264 g         1782 t
ORIGIN      
        1 ggcaagcgcg ccgagaccaa ggattacttc tcgctcttcc tcgcggagct cgccttggcg
       61 ctcgacggca aatggctctc gtgcagcata gaggcgagga tgcccgtcgc tgaccgctat
      121 accggcacgc cgcctgcaag cgcctaccaa ttcgccaacg acctgcccgc gatcggccgg
      181 gcctgcgacg cggtccgcgt catgacctac gaccagcaga ccgcggacca gaagctcaat
      241 gcgcttcgcg ccggcgcccc ctacttcccg gtggcggatg cgaagtggac cgagaaggtc
      301 atcaacctca tgtcccagga tatcccgaag agcaagctgt cgatcggcgt cgccacctac
      361 ggccgcgagc tcgaggtcac ggtctcgccg accggcaaat acacgtacaa gaacctctgg
      421 tccttcaatc cgggatacgc cgcccagatc gagaagaagt tcaaggtgaa gcgaagccgg
      481 aacgcggccg gcgagatgag tctcacgtat atcgataaga agcagaaggg cggccccacg
      541 cagaagcagc ttctcgcgct tgcgcccgcc ggcacgccta ccggcgagca ggtcgcggca
      601 ggcgccaagg cgtacgccgc caagaacaat acccccgtga ccttccggtt cctctcctgg
      661 agcgacgcgg gcgcgctcca aggccacgtt gcgctcgcgc gagcgctcgg ggtgaacggt
      721 atcgccgtct tcaagttcga cggcagcgag gacaagggca tctggaccca catgaagtaa
      781 cgctccgctg atgagaacgg ccggtctcgg gaccggccgt tctcatatgc tcttcacgac
      841 ccggagcgca gagttactgg catgaacacg agaccacgct ggtgggagac cgtgacgctc
      901 ccgacctacc cgaagctccg ggagaacctg cgcacggagg tctgcatcgt gggcggcggg
      961 ctcgccggcc tcatgagcgc atacctcctc gccaaggcag ggaggcatgt ggtcctgctc
     1021 gagaagaagg gcattgcctg gggggcaacc ggcgccacga ccgccttcct cgagcagctc
     1081 atcgacaccg acgtgaagga ccagatcgag atgatcggca aggagcatac caagcacgtc
     1141 tggcagtccc atggcgccgc gatcgacctc atcgagcgga tcgccaacga cgagggcatc
     1201 gagtgcgagt tcacccgctg tccggcctac gtcttcggca cctcccgcgg cgaggcccgc
     1261 gacctcaagg atgagttcgc cgccatgaaa ggcctcggat tcccggtctt cgaatacacc
     1321 aagaagctcc ccttcgacca gcgctacgct ttcgagatcc ggaaccaggc gaagtaccat
     1381 ccgctgaagt tcatcaatgg cctcctgccg aagctcgcgg atatgggcgt gaagctatac
     1441 gaaaagtccg aggtgacgaa gatcaccggc aagggcccct tcaaggtcca tacgaccggc
     1501 ttcttggtgc gtgcggacgc aaccgtcacg acgacgtacc agcccttcaa caacccgaag
     1561 gaggtcttcc tcaagaaggg gatgtacaag acgtacctcg tcgagttcag ggtcccgaag
     1621 gggaagtacc cggtcgcggt ctacgaagac gcggacaacc cctaccacta cttccgcatc
     1681 gatcgaaacg gcgatcacga tctcctgctg cttggcggcg aagaccatcg catcgagatc
     1741 aagatgaagg aggagaaagc gtacgcctcc ctcgaagaat acgcggagga gatcttcgga
     1801 acgcggtatc ccgtcactcg ccgctggtcc gggcccatcc tcgagcccat cgacgggttc
     1861 gcctacatcg gccgctatgc acccggccag tatgtcgcga ccgccttctc cggcaacggc
     1921 atgacctaca gcgcgatatc agccatgatc ttccgggacc tcatcaccgg gaagaagaat
     1981 ccgtatgcgc atctctacga gccatcgcga ttgccgagca tgacgcacct ctggatcaag
     2041 ggccgcgact acacggagga attgttccgt ggcgcgctta ccgatatctt caagaatcct
     2101 aagaaacgct aaccatgaaa accaccatct ggtccaagta cgggtacctt tggatcaccc
     2161 tcgtgctctt cctcgggagc ctcatcgggc actgggtctt cgcctggtac gccttcgtcg
     2221 acgagcagct cgcgcacggg caggccatcg aactgaacgc ctacttcatc gaggtgatgc
     2281 gcgacaccct cgagaactgg cagtccgagt tcctccagct catgtggcag gtcgccggcc
     2341 tcgcctacct cttctacatc ggctcgccgc agtccaagga aggggacgac cggctcgagg
     2401 agaaggtgga ccgcatcctg cttgcggtgg ataaggacgg cgagaagacc ctcaagcaga
     2461 tcgacaagcg ccacatgcgc gactaatcga agagggaagc gaggtacgta ttgatctccc
     2521 gctggtcgag gtcgtagaag gccttgccct cgtcgtggag cttggtcgcg agctcgcggc
     2581 ccacgtaccg ccagtgccag ggctcgtgga tgtagtagtt gttgccctca ggatacgaga
     2641 gggtgaagcc gtaccgatgc gcgttcttcg tgagccatgc gtacgcctcg gtcctcccga
     2701 agcccgtgaa cgcgctgccg agcgccgggg tcgtgaagtc gaccgtcgtg ccgagctggt
     2761 gctcggaata cccctggtcc gcggagaagc ggttggcgcc cgcgccgtac gcaaccgcgt
     2821 acccgctctt cagcgccgcc tgcgtcccga aggagcggta ggcggactgg atgcgcaact
     2881 cgatgccgtc atctgcagcg tcatcgatga gcgcctcgag cttgggcttc acgcgggcgt
     2941 gtatctcgag cgggcgagtg acatcaatga gatggctctt atcgatgagc gcgagctcgg
     3001 acggagagta gttctcattt aggaagtaca ccttcgaata cttagcgaga agctcgggat
     3061 cggtcgtggc gagcttctcg agcgtcccca ctgcccccga gagctccccg atctgccgct
     3121 cgaagaggcc cgtcttcgcg cgctcggcgg cgagcgcgtc cgcgagtcgg agccgctcgg
     3181 cctgtgcctc cccgagccgc tcctcgaggt ccttgatggc ggcatgcgcc tcgaggttcg
     3241 ctgcggcgta tgcatcggca tcgcggtcga gcgctgcgta tcggtagatg ccaaagccaa
     3301 gcgcgaggat gaggacgccg caggcggtcg ccagcgcgat gaaatgggag ggatggaggg
     3361 tcacgagggg gagtgtagca tacccgccgc acgctacagt tgatcttcat aattagtgtg
     3421 ttattacgct cgcaggcaac agggagagcg cccatgcagg caccggcggc agcaaccacg
     3481 gccgaatcgc gactaccgct acgcaagaag cagggagaca ggcgggtcgg gatccacgtc
     3541 ggcaagcgaa tcaggggccg ccgcaggctg ctcgggctca gccagcggca cgtcgccgcc
     3601 cgatgcggcg tcacgttcca gcagatccag aagtacgagt gcgccgcgag cgccatcaac
     3661 gcgaccatgc tctggaagct ggcacaggcg cttgacgtgc cggtgcacta cttcttcgat
     3721 gggctctccg acgaggacta agcacctctt cgcaatccgt cctttcgggg cccgacgatc
     3781 cgcgtcgggc ctacagcttt tccataccca aaaggtctgc tataagacta gggatatgaa
     3841 aaaaggaaag ttcgtgcccg gagccttcga tctccgagtc cgacgctcaa gcgccggact
     3901 cggccttttc accgagagcg ccatccctaa gggggcctgc atcatcgaat acgtggggaa
     3961 ggtgattacg cagaaggagg cggacacctc gaagagcaag tacctcttcg agatctcgaa
     4021 gaacaagacc atcgacggca agcccaaatg gaacaaggcg gggtacatca accatgcctg
     4081 caagccgaac gccgagagcg agatccataa cggccgcgtc ttcatcctcg cgacgcgcaa
     4141 catcacgccc ggcgaggaac tgacctacga ttacggcacg gagtacttcg acgagcacat
     4201 caagccgttt ggctgccggt gcgtgagctg caggaagaaa tagaggacgt tacgacagca
     4261 cgtcctcggc aagcgatcgc tcgatattcg tgagcgtgcg ctgcagccgc gcgagcgtct
     4321 tcgcgccatc gctatcgagg tcctgcgcgt accgctcgtt ccagtacggc aggggatcga
     4381 aggtgccgct cgcgtggttc aggtccacgt ccacgaagcg ctgccgtcct tcggcccgca
     4441 ctcgcttcgg ggccatatgg atataatcgg caagcggatt gccgcccgca aagccgatca
     4501 attcgcccgc cgctacctgc tccccgggca gcacgatcgc aagcagcacg tgcatgaagg
     4561 tgatctccac atggcacttc gtatccggga actcgtaggc atgcggcgca agcagcgtga
     4621 tgtgcaggtc cctcggatcc tcgcccgtcg atacctgccg tacgcgcgcg gcatagggcg
     4681 catggatcgc ttcgcctgcc ggggctgcga tatcgatccc gttatgatgc gtgaatccga
     4741 accgctcgta ccgcgggtcc tcgacgcccc aggcgtcggt cacgatatgc ggatagacag
     4801 ggtagtagag ggagagcttc atggagggat cctaagcgct cccgttagga gacttcgtga
     4861 agaaggtccg ccaagggcgc gatcgcgagc ggcacgatca tctcgcgctg cgagagcccg
     4921 ccgtggatgc cgagctgccc gaagaagacg tcgggcgcgt gcttgtacca cacgtggtac
     4981 ccctcgtagg ggaggatgag cacgtcgccg acgcgccgca tgaagcgcgc agaaggcgtc
     5041 ccgagcccgt agagcccccg atcgatcgcc tcattggtgg tgatcacctc cgccttgccc
     5101 tcgagctggc gcttcaggta gttgatggtc gccgcacgct tgccctcgta gatgtggagg
     5161 aagacgtcgt gcggggcgcc ggtcgggtag atggccttcc cgttcgggcc gtaggtgtag
     5221 ttggcttcga ggtccacgta gtcattcagg tagatgatgt cctcgttgcg gatggtcgcc
     5281 tggccgtggt ccgacgacag cacgaagagg gtatcgcgca ccccttcctg cgacagcttc
     5341 gcggtgaatg cctcgaggaa gcccgagagc tcctggagcg cgctgatgtg ctccttcgag
     5401 cgcgggccgt acacgtgctc gatgctgtcc acgcggtccc agtacaggaa gtggtaggcg
     5461 ggtcccgcct cctccgtgat cgtcgcggtc agcgtctgca tgaggtcgtc gaggtcacgg
     5521 aagggcctgc tcgccgagcc ccgctgcgag acggcggaat acgagctcac agcgatctcc
     5581 tccttgatat acacatagga gcacacaccg tcgtccgcga gctcctcgta gaacgtccgg
     5641 ccctcgtaga ggctctcggg gtccgcaccg agcgccgaga gcccctcgcg ctcgtgggag
     5701 aggtgcgcgc ggaagggcag gggctcgatg atccgatcga actcctcgaa gtacaccgtc
     5761 cactccggca ggccatgctc ctgcggggtc ttgttggtat gcagcgtggt gagcgcggcc
     5821 ggcgtcgtcg acgggaagac cgtggtgagg ggatagatgt cgcccctcgt cttcaggcgg
     5881 tggaagaagg gaagcacgga ctcatagtcc accatatgat cgaacccgag cccgtccacg
     5941 aagaagaaga gcacctgccg gtacgcgtgg ttgtaccgga gcgcgaaggg cagcgccgcg
     6001 cggtcgtcgg gaggcagccc gagccaaagc cggaccgagg gcgggatctc ggcgatggaa
     6061 tacgcgccgt agtacggata gatgaagctc tcgtgctgct tcgctcgctt tatggtgtgc
     6121 aggacatcgt ggtggaccat ggctagcgga tgatgtcgcc cagctccttg tacacgtaat
     6181 ccatgtggtt gatgtgcgcg tggtcgatga actggtcgat ggcgcccagg gggtagtgca
     6241 tgttcttcag cttcttattc ttgatggatt ccttgaggcc gtcgaagaag ccgctcaact
     6301 gcaggaccgg gtacccacgg ccatggaaat ccgtctcctc ggggtatacc ggcggggtga
     6361 ggttgagggc gttatgcatc tccccgagcc tgcggtacgc cttggtgagg agtttctggc
     6421 gcggcagcca tcgcttctcg tggagtatct ggaggaggag gggatacagc tcggggccgg
     6481 accggagcca cctctggaac gcggtcccga accatttcga atatggggcg taccggcgct
     6541 ccatcaggaa gcacatgaac atgatcttct gtacggtgcg cgcggtgacg accatcgacc
     6601 cgacctcatc cccctcctcg ccgctgcgcg agggcgactg gatctcgtcc gcgatcttcc
     6661 accactggat gtggagcagg tatttccata tatcgtccgg gtaccaggcg aactgcctcc
     6721 ggtacttctc gacgtccagg tcgtcgcggt agagctgccc gcccgtcacc tcgatcaggg
     6781 cctgctgcgg aaacagcagc caatcccgga acgagggctg tttcgagatg tcgaagccca
     6841 gatagtgctg gaagaaggag cggatggtcc agaaccggaa gatatggttc accggcccgc
     6901 gcttcttcgc cttcggctgg tgcttgaggt aatagtcgcc ctcggtgaag tgcgtcggga
     6961 atcctttata gctggtaggc aggtgcttcc gcaggagggc atcgacccgg ttcttctcga
     7021 cgatgaaatc gaggtccgcg aagaagatgt ggaggtaggg gccccagtcg tggtcggtcg
     7081 aggtcggggt atcgaacccg agcacgtccg agccgtgccc cacgagcccg gccgaatact
     7141 ggagccctgg gcagtgctgc tcgatgaggg gcctgaccac gtcgaaatag aagtcgcggg
     7201 tgagcgcgag ccccgggatg aatttgggca tagtccctct gtagtaacgc gtccgttgta
     7261 aagccctcgt gaagggccga ttcatgtgcc gctcaccccg tccctggtat gtaagggcta
     7321 tgaagcgatc cgcgatcgag gtgaagcagg cgaaggaggc gtccgcgagg aagggaaccg
     7381 caggacgccg cgaccataag cagggcgccc caagctcgaa caagggcacg ggcggcaagc
     7441 tcgcgatgta ggagcgatag cataaacccc gcctcgggcg gggttgtatg ccggtgcgaa
     7501 gcgcgcccta gcgattgccg ccgcggcttg cctggccacc cttcctgcct gcctcgcgtg
     7561 cttcctctga ggtgaactcg tgcgccgtcc ccttctggtg ggctgccttg ccgcccttgc
     7621 ttgcaatctc ccgctgcttc tcctcgtcca tcgccgcgaa tccgcgacga ctcttcccgt
     7681 tgttgttact tgccataccc ttacggttgg ttagattggt aggccacgac tatagcgggc
     7741 ctgccgtgaa ccgtaagtga aatctactcg agcacgcgat ccgcgagccc gtacgcgacg
     7801 agctcgagcg gcgtgaggat ggtgttcttc tccatcatgc gcagcacctg gtcggtatcg
     7861 aggcggccag cggagcgctc ggcgagtatc gaggcgtact ggaagtcctt caggcggtcc
     7921 tcgcgcagca tggcgtccat ttcgtcggcg gtgtagcgct tgccttcgct gaaatgccgg
     7981 cccgcaacat gaagcaggag cgtcgtgtgc ggggtgacgt agcgggtatc gcccgcaagg
     8041 aagatgatga tgccggagga atccacgtcc ccggagccga tcgtggtgag gcggggcttc
     8101 aggatatagc gcatggtgtc gtagaagctc atcgcggtgc cggtcgggcc tcccgcggag
     8161 gtcacctgga gcgtgatagg cgtctcggga gcggcaatga cgagctcgcg catctcctcg
     8221 atgacgcgct cattggtcgc gtagttgacc acgccgaagt actcgatggt gcgggccgga
     8281 tcggtgacga tcgcccgctt cttcgtacgg atggcctgtt tctgccgcgt tttctccatg
     8341 aacccagtat gaaggggaga tgagcgagga gcaagcagag ccattgcgga tggggaaaac
     8401 cgcacgaggt ccgtgcggtt catccgtgca cccggggcgc agcgccttag gtgatggccg
     8461 cgaagaggcg cttcatagtg gactcgcgga gctcttcctt gcgcgcctcc atctcaggca
     8521 ccatctcctc gagccgctcc gtcccgatga tctcctccat gcggggcatg gcggtgctct
     8581 cctcctcggc cacgtggtgc aggacggact ccatcatggc cttcatggcg ggcgcgtact
     8641 cctcgctgtc cacgtccatc atgctgagct tggcgatgta taccttcacg ccctcatgct
     8701 cggcgcgggc ctcgtccgcg agcgccttct cgccggcctc ctcgagcaag gggtaataga
     8761 actcctcctc gacgatggcg tggacggtga ggtcggtgag gatctgcttg gcgagctcct
     8821 cccgctcctc gccctcggcg gcgacgaagt cggtgtacgc gtcctcgagc tcgcggtgat
     8881 cctgcatcag tacatcatta gcagtcatgg tttgttgtta cgttcgtaat gcccgcagtc
     8941 tgcggcaggc gccgtgaggg ggcgatgaat tcaaggtata taaaaggaaa ggccgccgag
     9001 ctctggggag ctcgacggcc gatccgcgtc gtggtcgtag gctaggtcag gcgtaggcga
     9061 ggcgccgccg cgcacgaagc gcagcgccgg tgaagccgaa gcccaggatc atcaggcccc
     9121 aggtcgccgg ttcgggcacc ggagcggtgg ccacgaacgg gcggccgtgg tagccggtga
     9181 cccggaagtt cacgtcgatg tcgtcgccga cgatcaggtt cccgccgccg gtcgccgcca
     9241 cgggcccgag cacctggtcg aggctgtcga gcgacggttg cagggccgag atcgtgaacg
     9301 cgcagcccga gcagctcgtg gtgaacgtca ggatgccgtc gggccggaag aggccgagcg
     9361 cctgcgccga ggacgtgaag tcgaaggtgc cgatcccggt gatgtcgagc tccgccgaga
     9421 cgaacgggtt gtccgtcacg gagacggcga cggtgtcgtt gtcgtcgtcc gtcgtgcggt
     9481 tgacggccgg gtcgaagacc aggtcgaagg tgaacgcgtt gctgtcgctt ggcacgaagc
     9541 tcgggatggc gttgtcggcg aagccggtca gggtcacatg gaagtactca acggcgctcg
     9601 cgggcgaggc cgcaagcgcg agggcggcgg cagccgccgc gaggctgatc ttcatcgggg
     9661 ttgatccttg aagcctatgg gagacgcgga cgaacagatg tccgtcgtag ttctaccaca
     9721 tccgaggatg ttctgcaagg catggaaaag ccggaccctt gcgggcccgg ctggaagtcg
     9781 aacgctgtac ttgttgaggt cggagccgtc tacgcggctg ccagaccgtg ccggcgacgc
     9841 cggacgaggc tgcccacggc gccgaagccg aggatcatca tcgcccaggt ggcgggttcg
     9901 gggacggcgc cagccccgag gatctcgaga ttgccgctcc ccacgacgtt gccgtccagc
     9961 aggtaggtga cactgaagtt cgtcaccgcg aactggttga agtaggacgt cgcatcagcg
    10021 atggacgcct gc
//
LOCUS       DASPKG010000095         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_404115, whole genome shotgun sequence.
ACCESSION   DASPKG010000095 DASPKG010000000
VERSION     DASPKG010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404115"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VEA36_03835"
     CDS             complement(<1..795)
                     /locus_tag="VEA36_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HYF29465.1"
                     /translation="MIEEKNPPARPAPVATFTVDPGHPMADKLDAIAAPPRVGDLVEG
                     TIIELVRGRLYVDLPPFGTGIIYGREYLNAADVLRKANPGDTITAKVVDPSGIEGYIE
                     LSLKEARQAAIWSEAEQAIAAQTLYALTVEEANKGGLMLSWQGIQGFLPASQLSKEHY
                     PRVPEGDKDKIFAELKKLEGMPLSVRIITADAKEGKLIFSERAGAEEEEKASLIDKYQ
                     VGDVVEGEVTGAVDFGVFVKLEPGLEGLVHISELDWGLVEDPRALFK"
     gene            complement(970..1101)
                     /locus_tag="VEA36_03840"
     CDS             complement(970..1101)
                     /locus_tag="VEA36_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29466.1"
                     /translation="MAPTPPSDHMQRDWVRRNYRQDGGHDDDGGDRPKKKPPQPIRH"
     gene            1312..1692
                     /locus_tag="VEA36_03845"
     CDS             1312..1692
                     /locus_tag="VEA36_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29467.1"
                     /translation="MKKFSFVLALALMFSPLAASAATFSVNPDPIYRNETFDVTCSGL
                     GSSGNEMVTLVMFTPDQSMFMFTSSCDPIEQTMNESLLGYGTYTWSIYDDNSWGGDGS
                     YSSFMEGPWVHETGSFEMIDAERP"
     gene            1839..2483
                     /locus_tag="VEA36_03850"
     CDS             1839..2483
                     /locus_tag="VEA36_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYF29468.1"
                     /translation="MVITHHGAQCFKVTFGDLTLVFDPISKQGTLPAVRFGADIALSS
                     RNHPDMNGIEEVTFGNKEPFAITGPGEYEHSGVTIEGFLTKSQYGLPKGQMDAVNTVY
                     AVNLEGMTLVHLGALSDPVLSHEAREAIDEIDILFVPVGGDGVLSPSEAAKLAVSLEP
                     HIIIPMHWSGMGEDRALDAFLKESDSTKTEKVEKLTLKKKDAAEKDGAIVVITP"
     gene            2502..>2847
                     /locus_tag="VEA36_03855"
     CDS             2502..>2847
                     /locus_tag="VEA36_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29469.1"
                     /translation="MAALSEHLERIREKPHHVRRQIAFGTSLTITALVAIGWVGALVS
                     TPKLAIDPNLADEELSLANAPTMTGGSFSELVGAAGAAFGATTSPAKVMVVDTKTSST
                     LDVKRDNHNDTKE"
BASE COUNT          544 a          883 c          872 g          548 t
ORIGIN      
        1 cttgaagagg gcgcggggat cctcgacgag gccccaatcg agctcagaga tgtggacgag
       61 cccttcgagg ccgggctcga gcttcacgaa gacgccgaag tctaccgctc ccgtgacctc
      121 gccctcgacg acgtcgccca cctggtactt atcgatgagg gacgccttct cctcctcctc
      181 ggcgccggcg cgctcggaga agatgagctt gccctccttg gcgtcggccg tgatgatccg
      241 gaccgagagc ggcatgccct cgagcttctt cagctccgcg aagatcttgt ccttgtctcc
      301 ttcaggcacc cttggatagt gctccttgga gagctgggac gcggggagga agccctggat
      361 gccctgccac gagagcatga ggccgccctt gttcgcctcc tccaccgtga gggcgtagag
      421 ggtctgtgcg gcgatcgcct gctcggcctc gctccagatc gcggcctggc gcgcctcttt
      481 cagcgagagc tcgatgtagc cctcgatgcc ggagggatcg accaccttcg cggtgatggt
      541 gtcgccggga ttcgccttac gcaaaacgtc cgcggcgttc aagtactcgc ggccgtagat
      601 gatgccggtc ccgaacgggg gaaggtccac gtagaggcgg ccgcgcacca gctcgatgat
      661 ggtgccctcg accaaatcac ccacccgggg cggggcggcg atggcatcga gcttgtcggc
      721 catcgggtgg ccgggatcga cggtgaacgt ggcgaccgga gccggacggg ccggcgggtt
      781 cttctcttcg atcatgaata tgcgcatccg acttttgcgg tacgtactgg aatccttgcg
      841 gcttccaggg ttagcgtacg cgcgactcgt cgatgcgatg aacaggagga atatagcaat
      901 aaagcatcaa aaatggaagc ccccagcccg cgtatgcgga gccgggggct gggggcctgg
      961 ccctagagct cagtgccgta tcggctgcgg cggcttcttc ttcggacggt caccaccgtc
     1021 atcatcgtgg ccgccgtcct ggcggtagtt gcggcggacc cagtcacgct gcatgtggtc
     1081 gctcggtggc gtcggagcca tcttacctat ccctcattag gaaaacagaa ctgccctcac
     1141 gaagtgggac agccaggaga gcataccatt tgagcgctcc acccgctatg ccgaagcgaa
     1201 ccttcagggt ttctctatta aatattcatg aaagcagcat tacctccatg ctaccgtccg
     1261 accgcatgca cgccatgcaa attaccaccg ggcattagat atcccataat tatgaaaaag
     1321 ttctcctttg ttcttgcgct ggcgcttatg ttttctccac ttgctgcctc agccgcgact
     1381 tttagcgtga atccggaccc gatctaccgc aatgaaacct ttgacgtgac gtgctcgggt
     1441 ctgggaagct cggggaatga aatggtaacg ctcgtgatgt ttacacccga ccagtctatg
     1501 ttcatgttca cctcgagctg cgacccgata gagcaaacga tgaatgaatc cctgctcggc
     1561 tacgggacgt acacgtggtc gatctatgac gacaattcgt ggggcggcga cggcagctac
     1621 agctccttca tggaaggtcc gtgggtccac gagaccggct ccttcgagat gattgacgcg
     1681 gagcggccat aggcaccgtc ccgtgtgatg ccacacagag cccttcgggg ctctgtgtgt
     1741 ttgcataaag attccgtgtc ccgaggggcg cgctacggac aagcggcggt cggaaacggt
     1801 gggaaaacga tgagtttcgt gctataattt gcgcatctat ggtgatcacc caccacggcg
     1861 cccagtgctt caaggtcacc ttcggcgacc tcacgctggt cttcgacccc atctcgaagc
     1921 agggtacgtt gcctgcggtc cgcttcggcg cggacatcgc gctttcctcc cggaaccacc
     1981 ccgacatgaa cggcatcgag gaggtcacct tcgggaacaa ggagccgttt gcgatcaccg
     2041 gccccggcga atacgagcat tccggcgtta cgatcgaggg cttcctcacc aagagccagt
     2101 acgggctgcc caaggggcag atggatgcgg tcaatactgt gtacgcggtc aacctcgagg
     2161 gtatgacgct cgtgcacctc ggtgcgctct cggaccccgt gctctcgcac gaggcccggg
     2221 aagcgatcga tgagatcgac atcctcttcg tgccggtcgg cggggacggc gtgctctccc
     2281 cgtcggaggc cgccaagctc gcggtctcct tggagcccca catcatcatc cccatgcact
     2341 ggagcggcat gggggaggac agggcgcttg acgcctttct taaggagtcc gacagcacca
     2401 agaccgaaaa agtcgagaag ctgaccctca agaagaagga cgccgctgag aaggacgggg
     2461 ctattgtcgt tatcacgccc taggcgcata cttttgcata tatggccgcc ctctccgaac
     2521 acctcgagcg catccgcgag aagccgcacc acgtgcgtcg gcagatcgcg ttcgggacct
     2581 cgctcacgat cacggcgctc gtcgcgatcg gctgggtggg ggccttggtc tccaccccga
     2641 agctcgcgat cgaccccaat ctcgcggacg aggaactctc gctcgccaac gcgcccacca
     2701 tgactggggg aagcttctcg gagctcgtcg gcgccgcggg cgcggccttc ggggcgacca
     2761 cgagcccggc gaaggtcatg gtcgtggata cgaagacgtc gtccacgctc gacgtgaagc
     2821 gagacaacca taacgatacg aaggaga
//
LOCUS       DASPKG010000096         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_2027845, whole genome shotgun sequence.
ACCESSION   DASPKG010000096 DASPKG010000000
VERSION     DASPKG010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027845"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1741
                     /locus_tag="VEA36_03860"
     CDS             <1..1741
                     /locus_tag="VEA36_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HYF29470.1"
                     /translation="EKEAQINAEIDRLRHAATQALLTRRDVIIVASVSAIYGLGSPAE
                     YEKGHVKLAVGQEENRAALIRKFINIYFERTNADLEPGLIRAVGNAVELMPTNERAIY
                     RVTFDVDVIARIEILDPVSRARTGEPASAFIFPAKHFVTSEDQRNRALEDIRRELDER
                     LAALKREEKVLEHERLKRRTLHDISLIRELGYTSGIENYSRHFDGRAPGEPPHTLLSY
                     FPHTQDGSADFLTVIDESHVTIPQIGGMFAGDRSRKDVLIEHGFRLPSARDNRPLTFE
                     EFEERVGQCIYTSATPGPYEKEHSVPPLGQVAEQIIRPTGLIDPELIVRPIVETGDYK
                     GQVADFIEEAATVIAGGGRVLATTLTKQMAEDLAEFLREKKVKAEYLHSDIKTIERIE
                     ILTAFRKGEFDILVGVNLLREGLDLPEVELVGILDADKEGFLRSETSLIQTIGRAARN
                     VLGRVILYADVMTGSMERAIGETNRRRERQLAYNAEHGITPITIKKEIRDIAASMRTE
                     HQKTVDSLLAFDAKLYEEDPEAFLKEKRKQMADAVEALDFETAAILRDEIYALEGKEA
                     PAKKKAPARRRKR"
     gene            1762..>2197
                     /locus_tag="VEA36_03865"
     CDS             1762..>2197
                     /locus_tag="VEA36_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29471.1"
                     /translation="MSLRTIIFVLILGALVLTIGSMAIAYLLMIGQVEKLFPNSSAVQ
                     AVDSLKLLFIQYSSILLAFITVIATIAFLILSRYIVEPVQDMVTAIQAFADRSERIDV
                     AEFRDAPVEVAELAKVFNDFTIKVDDAHARDVEVSRMKSDFIS"
BASE COUNT          432 a          699 c          707 g          359 t
ORIGIN      
        1 cgagaaggag gcgcagatca atgcggagat cgatcggctg cggcatgcgg cgacccaggc
       61 gttgctgaca agacgggatg tgatcatcgt cgcgtccgtg tccgccatct acgggctcgg
      121 ctcgcccgcg gaatacgaga aggggcacgt gaagcttgcg gtggggcagg aggagaatcg
      181 cgcggcccta atccgcaagt tcatcaacat ctacttcgag cgcacgaacg cggacctcga
      241 gccaggcctc atccgggcgg tcggcaacgc ggtcgagctc atgccgacca acgagcgcgc
      301 catctaccgc gtcaccttcg atgtggacgt catcgcgcgc atcgagatcc tggatccggt
      361 ctcgcgcgcg aggacggggg agcccgcgtc cgccttcatc ttccccgcca agcacttcgt
      421 gacgagcgag gaccagcgca atcgcgcgct cgaggacatc cgccgcgagc tcgatgagcg
      481 ccttgcggcc ctgaagcgcg aggagaaggt gctcgagcac gagcggctga agcgccggac
      541 cctgcacgac atatcgctca tcagggagct cgggtacacc tcgggcatcg agaactattc
      601 gcggcacttc gacggccgcg cccccggtga gcccccgcat acgctgcttt cgtacttccc
      661 gcacacgcag gacggctcgg cggacttcct gacggtcatc gacgagtcgc acgtcaccat
      721 cccgcagatc ggcggcatgt tcgcgggcga ccgctcccgc aaggacgtcc tcatcgagca
      781 cgggttccgg ctgccctctg cccgcgataa ccgcccgctc accttcgagg agttcgagga
      841 gcgcgtcggg cagtgcatct atacctcggc gacgccagga ccctacgaga aggagcactc
      901 ggtgccgcct ctaggccagg tggccgagca gatcatccgg ccgacggggc tcatcgaccc
      961 ggagctcatc gtccgtccca tcgtcgagac gggcgactac aaggggcagg tcgcggactt
     1021 catcgaggag gcagcgaccg tgatcgcggg cggcgggcgc gtgctcgcga ccacgctcac
     1081 gaagcagatg gcggaagacc tcgcggagtt cctgcgggag aagaaggtga aggccgaata
     1141 cctgcattcg gatatcaaga cgatcgagcg catcgagata ctcacggcat ttagaaaggg
     1201 agagttcgat atcctcgtgg gcgtgaacct cctgcgcgag gggctcgatc tccccgaggt
     1261 ggaactggtc ggcatcctcg acgcggataa ggagggattc ttgcgcagcg agacctcgct
     1321 catccagacc atcggtcggg cggccaggaa cgtcctcggg cgcgtcatcc tgtacgcgga
     1381 cgtcatgacg ggctccatgg agcgcgcgat cggggagacg aaccgccgcc gcgagcggca
     1441 gcttgcctac aatgcggagc acggcatcac cccgatcacc ataaagaagg agattcggga
     1501 catcgccgcc tcgatgcgga ccgagcacca gaagaccgtg gattcgctcc ttgccttcga
     1561 tgcgaagctc tacgaggagg atccggaagc cttcctcaag gagaagcgca agcagatggc
     1621 ggatgcggtc gaggcgctcg acttcgagac cgccgcgatc ctgcgcgatg agatctacgc
     1681 gctcgaggga aaggaggcac cggcgaagaa gaaggcaccg gcacgcagaa ggaagcgata
     1741 gccgcgtgct agcatagcga catgagcctg cgtacgatca tcttcgtgct tattctgggc
     1801 gcgctcgtgc tcaccatcgg ctcgatggcg atcgcgtacc tcctgatgat cggccaggtg
     1861 gagaaattgt tcccgaattc ctccgcggtc caggccgtgg atagcctgaa gctcctcttc
     1921 atccagtatt cctcgatcct gctcgccttc attacggtca tcgcgaccat cgccttcctc
     1981 atcctctccc ggtacatcgt ggagcccgtg caggacatgg tcacggcgat ccaagcgttc
     2041 gcggaccgga gcgaacgcat cgatgtggcg gagttcaggg atgcgccggt cgaagtggcc
     2101 gagctcgcca aggtcttcaa cgacttcacc atcaaggtgg acgacgcgca tgcgcgcgat
     2161 gtcgaggtct cccgcatgaa gtcggacttc atctcga
//
LOCUS       DASPKG010000097         3276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U12030
            k141_1486807, whole genome shotgun sequence.
ACCESSION   DASPKG010000097 DASPKG010000000
VERSION     DASPKG010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944953
            Sequence Read Archive: SRR7003765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 01:34:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 777
            CDSs (total)                      :: 741
            Genes (coding)                    :: 740
            CDSs (with protein)               :: 740
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 32
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486807"
                     /isolate="SMAG_U12030"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /gene="uvrA"
                     /locus_tag="VEA36_03870"
     CDS             complement(<1..1323)
                     /gene="uvrA"
                     /locus_tag="VEA36_03870"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HYF29472.1"
                     /translation="MTKKKPDEGHTQARGEDWIRVKGARTHNLKNLTTEFPRGAMTVV
                     TGLSGSGKSSFAFDTIFAEGQRRYVESLSAYARQFLNQMQKPDVDEITGLSPAISIDQ
                     KSRSNNPRSTVATVTEIYDYLRVLYARAGQPYCVNHDVPISKLSKEEMIHLIMGKVAK
                     RAARADKQVMGVAVDRTQVIIYAPVVVGRKGEYYQLLYDLLGKGFERVVIDGKVQPLR
                     EKIVLDRNKRHDIDAIVDAIFVSEFEKSPDAARERLSEALEIALKESDGLVKIGHADG
                     SQETLSARFICPEDGMSFPEVEPRLFSFNSPYGACPTCNGLGTKSLFSSDPCPACEGK
                     RLRPESLRVYLTGKKEKGLGKNVVDFTNFSIQEAKDFMDHLELSEMRMEIARPIIREI
                     TSRLEFMLNVGLDYLTLNRSAATLSGGEAQRIRLASQLGTGLVGALYVL"
     gene            1486..2172
                     /locus_tag="VEA36_03875"
     CDS             1486..2172
                     /locus_tag="VEA36_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYF29473.1"
                     /translation="MSSIGVAAPATSAIVPAVPVVARNVIRDAGDIVSVRIPADARQG
                     CYEGDGLVIPYFRYSPTVPGGCVHCFVHTDDESLRGRSLRAQATIMEKVLADGRRYYY
                     VDLLPVQAEVPPTHRLVITGCALVEDGLIFATPAPIYGMIMLTAPSAKLIEFPRLPGR
                     KPEPKAKARKPEPVRKLEAKAAQPKDTQLDRLLSQGWEIKVDWGDRVVLTKLKKGEEV
                     EMTHHRPKKK"
     gene            complement(2240..2632)
                     /locus_tag="VEA36_03880"
     CDS             complement(2240..2632)
                     /locus_tag="VEA36_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYF29474.1"
                     /translation="MADTKFILVVEDDPVLKNLLGHTFAGKYQTLYASDGNEALALFE
                     SNKPALVLLDLMLPTMDGFAVLQAIRGRTDELKNVPVIVVSNLGQQADIEKAKSLGAN
                     DYLIKAEVPVEEIVAKIEGMLAQSKPAA"
     gene            complement(2648..>3276)
                     /locus_tag="VEA36_03885"
     CDS             complement(2648..>3276)
                     /locus_tag="VEA36_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYF29475.1"
                     /translation="GIRWALEALEKEQLTESQQALVKSAKDKSHQLVTIVGTLLDISA
                     IESGKNIYKFEGVDLATLAHEVAKDFGELAARSRVSLYFVPSEVPLPPAKADRERIKW
                     VMNNVIENAIRYTPAGGSVSLWLEEGAGRIFLHIKDTGIGIKPEDRGNIFERFYRAGN
                     AIAKENAGNGLGLYIARTIASDHGGDLNFEPNKEGPGTTFTLSLPVFA"
BASE COUNT          582 a         1068 c         1013 g          613 t
ORIGIN      
        1 gagcacatag agcgctccca cgagccccgt gccgagctgc gacgcgagcc gtatgcgctg
       61 cgcctccccg ccggacaagg tcgcggcgga gcgattcagg gtgagataat cgagccccac
      121 attcagcatg aactcgagcc gcgaagtgat ctcccggatg atgggccgcg cgatctccat
      181 gcgcatctcc gagagctcga ggtggtccat gaagtccttc gcctcctgga tggagaagtt
      241 ggtgaagtcc accacattct tgccgagccc cttctccttc tttcccgtga ggtacacccg
      301 cagggactcg ggccgcagcc gcttcccttc gcaggccggg cagggatcgc tcgagaagag
      361 ggacttggtc ccgagcccgt tacaggtcgg gcaggcgccg tacgggctat tgaaggagaa
      421 gaggcgcggc tccacctccg ggaaggacat gccgtcctcg gggcagatga agcgggcgga
      481 gagggtctcc tgcgacccgt ccgcatgccc gatcttcacg agcccgtcgg attccttgag
      541 cgcgatctcg agcgcctcgg aaagccgctc gcgggccgca tccggactct tctcgaactc
      601 ggagacgaag atggcgtcca cgatcgcgtc gatgtcgtgg cgcttgttgc ggtcaagcac
      661 gatcttctcc cgtaagggct gcaccttccc gtcgatgacg acccgctcga atcccttgcc
      721 caagagatcg tagaggagct ggtagtactc gcccttgcgg ccgaccacca ccggcgcgta
      781 aatgatgacc tgggtgcggt ccaccgccac ccccatcacc tgcttatcgg ccctggcagc
      841 acgcttcgcc accttaccca tgatgaggtg gatcatctcc tccttggaga gcttgctgat
      901 cggcacgtcg tggttcacgc agtagggctg gccggcgcgc gcatagagga cgcgcaggta
      961 gtcgtagatc tcggttacgg tcgccaccgt cgagcgtgga ttgttcgagc gcgacttctg
     1021 gtctatggaa attgccgggg aaaggcccgt gatctcatcc acgtccggct tctgcatctg
     1081 gttgaggaac tgccgggcat atgcggagag ggactccacg taccgacgct ggccctcggc
     1141 gaagatggta tcgaaggcaa atgatgactt tccggagccc gagagaccgg tgacgaccgt
     1201 catcgcgccc cgcgggaact cggtcgtgag gttcttgagg ttatgggtac gcgcgccctt
     1261 cacgcggatc cagtcctctc cgcgcgcctg cgtatgcccc tcatcaggtt tcttcttggt
     1321 catattacgg aaatgccccg gtccgtggcg ggaccggtac ggtatgacta tagcatttgt
     1381 gttgaagttc gcctgaagtg ccgacgcgta attgacatat tccaatagat atgtaaaata
     1441 tatatcgggt gctcatgccc agctctagaa acaaaggata ccgccatgtc gagcattggc
     1501 gttgccgcac cggcaacctc ggcaatcgtc cctgccgtcc ctgtcgtcgc ccggaacgtc
     1561 atccgagatg cgggggacat cgtgtccgtc cgcatccccg ctgacgcccg ccagggctgc
     1621 tacgagggcg acggcctcgt gatcccttac ttccggtact cgcccaccgt ccccggcggc
     1681 tgcgtgcact gcttcgtgca tacagacgac gagtcgctgc gcggccggtc cctgcgggcg
     1741 caggcgacca tcatggagaa ggtgctggcc gacggccggc gctactacta cgtcgacctg
     1801 cttccggtcc aggcggaggt cccgccgacg caccggctgg tcatcaccgg ctgcgcgctg
     1861 gtggaggacg gcctgatctt cgccaccccg gcgccgatct acggcatgat catgctgacg
     1921 gcgccgagcg ccaagctgat cgaattcccg cgattgccgg ggcgtaagcc cgaaccgaag
     1981 gccaaggcgc gcaagcccga gccagtccgc aagctggagg cgaaagccgc ccagccgaag
     2041 gacacccagc tcgaccgcct cctctcccag ggctgggaaa tcaaggtcga ctggggcgac
     2101 cgggtcgtcc tcacgaagct gaagaagggc gaagaggtcg agatgaccca ccaccgcccg
     2161 aagaagaaat aggagcaggc gcgagcccgc ttacactccc gccgggtacc tacgaggtac
     2221 accggcgggc tttttatacc tacgcagccg gcttggactg cgccagcatc ccctcgatct
     2281 tcgccacgat ctcctccacc ggcacctccg ccttgatgag gtagtcgttg gcgccgagcg
     2341 acttcgcctt ctcgatgtcc gcctgctgac cgaggttcga gaccacgatc accgggacgt
     2401 tcttcaactc atcggtgcgg ccccggatcg cctggaggac cgcgaacccg tccatggtcg
     2461 ggagcatgag gtccagcagc acgagcgcag gcttattgct ctcgaagagg gcgagcgctt
     2521 cgttgccatc ggaggcgtaa agcgtctggt acttcccggc gaaggtatgc ccgaggaggt
     2581 tcttcaggac cgggtcgtcc tcgaccacca ggatgaattt cgtgtctgcc atagctcgag
     2641 tatagcacta ggcgaatacc gggagcgaca aggtgaaggt ggtccccggg ccctccttgt
     2701 tgggctcgaa attcaagtcc ccaccatggt cgcttgcgat ggtgcgggcg atatagagcc
     2761 cgaggccatt ccccgcgttc tccttcgcga tggcgttccc cgcccgatag aaccgctcga
     2821 agatgttccc ccggtcctcc ggcttgatcc cgatgcccgt gtccttgatg tggaggaaga
     2881 tgcggcccgc tccctcctcg agccagaggc tcacgctccc gccggcaggc gtgtaccgaa
     2941 tcgcgttctc gatgacgttg ttcatcaccc acttgatgcg ctcgcggtcg gctttcgcgg
     3001 gcggcaaggg cacctcggac ggcacgaagt agagggaaac ccggctgcgc gccgcgagct
     3061 cgccgaaatc cttggccacc tcatgcgcca gggtcgcaag gtccaccccc tcgaacttgt
     3121 agatattctt ccccgactcg atggccgaga tatcgaggag cgtgcccacg atggtcacga
     3181 gctggtggct cttgtccttg gcggacttca ccaaggcctg ctggctctcg gtgagctgct
     3241 ccttctcgag ggcctcgagc gcccagcgga tgcccg
//
