LOCUS       DASPTF010000001         6128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1151701, whole genome shotgun sequence.
ACCESSION   DASPTF010000001 DASPTF010000000
VERSION     DASPTF010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151701"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VEH48_00005"
     CDS             <1..267
                     /locus_tag="VEH48_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17796.1"
                     /translation="QYAPLSVWQQVASVFSATEGAFDNVPIENLKAAQDAMLADIEQN
                     HEPVITELSKGDKPSEASKKAILAAAAKIAKQYRKVELKDEAES"
     gene            267..1127
                     /gene="atpG"
                     /locus_tag="VEH48_00010"
     CDS             267..1127
                     /gene="atpG"
                     /locus_tag="VEH48_00010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HXY17797.1"
                     /translation="MAQTRVLQRRIRSVRNAKQITKALEVVAASRMRKVIQLVAGSRS
                     YGELAAGIMRHIAANPEAAAHPYFNPEAGKPTLYVIINSDRGQAGAFNANIFSLAITS
                     FKAEKIRPQVVVFGRKGTRYFAHLAGIDLRGAYEDIGDMPDVNVFAPVMEMIQTELAG
                     GEIGRVVILYTQFLSSLSQKATSFQLLPIDLSAAGDAEPAKVYEFEPEVKDVLAEAIR
                     LYAEAKLMQAKIESAASEQAMRMVAMGNANRNASDLIDNLTLELNATRQAAITQEIAE
                     ITGGAAAIAQ"
     gene            1167..2639
                     /gene="atpD"
                     /locus_tag="VEH48_00015"
     CDS             1167..2639
                     /gene="atpD"
                     /locus_tag="VEH48_00015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXY17798.1"
                     /translation="MPETTKTKPATKKGVITQIVGVVIDVEFEGHLPSIYNALTVDLD
                     GKTLLMEVAQHLSRQAVRAVALGPTDGLARGTTVTDTGAPISVPIGEPTLGRMFNVIG
                     EPIDGKPVPAKAVLGPIHRQAPPLTDQNPKVEILETGIKVIDLICPITKGGKVGLFGG
                     AGVGKTIFITELINNIAKFHKGRSVFAGVGERTREGNDLYFEMQNSKVLDKTSLVFGQ
                     MNEPPGARLRVGLTGLAIAESFRDAGNDVLLFIDNIFRFTQAGSEVSALLGRLPSAVG
                     YQPNLATEMGTLQERITSTTKGSITSVQAVFVPADDLTDPAPATTFAHLDSTVVLSRA
                     LTELGLYPAVDPLDSTSTILDPETVGQEHYEVAREVQRVLQRYKDLQDIIAILGMEEL
                     SEEDKKLVARARRIQRFLTQPFFVGEVFTNIPGVYVPLAETIKGFKEILDGKHDSKNE
                     QAFYMKGAIDQVKDDKQSSKQPETSDKEIASNDKQPEETP"
     gene            2639..3061
                     /gene="atpC"
                     /locus_tag="VEH48_00020"
     CDS             2639..3061
                     /gene="atpC"
                     /locus_tag="VEH48_00020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HXY17799.1"
                     /translation="MRFQLVASSGLKFDDEAYEVMVPTKAGVISIFEDHMPLISSAQP
                     GVLSIRKKAGDNDSSLEHFAINGGMVEVDGKTVSFVADDVTTADEISEKDAEAALAFA
                     EDLVKNADTQSALHEAHRTLQHSSAQLHIARLKRRHHQ"
     gene            complement(3064..3387)
                     /locus_tag="VEH48_00025"
     CDS             complement(3064..3387)
                     /locus_tag="VEH48_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17800.1"
                     /translation="MSKAKVNPGFAAIEALLILAIVAIIGFVGYTVYHDRNNVNKLNN
                     ESAAQQVAVPKSSTSTSTSSPSSVPAAVNNTTDLNSAANTLNADNPNDNSSDLNQLNS
                     QSSAF"
     gene            complement(3387..3944)
                     /locus_tag="VEH48_00030"
     CDS             complement(3387..3944)
                     /locus_tag="VEH48_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17801.1"
                     /translation="MLKTSLKAKLLAGSGALAIAGSTLVVAIPAFAIATTSSGPAAVA
                     AGTRLDAAKLKLCTDRVNVIDSILKRVADRGNRHLTLISDVATKVETFYSKSGKTLDN
                     YDSLVATVNSDKTAAQAAVSQVQSDSTSFNCSGSDPVGNLQTFKADVQAEITALQNYK
                     AAVQALIAGVKSVVEPSSISGGGSQ"
     gene            complement(4145..5068)
                     /locus_tag="VEH48_00035"
     CDS             complement(4145..5068)
                     /locus_tag="VEH48_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17802.1"
                     /translation="MLALFAAVTAAMAFSATASNAGGWHHGGGTVTVTQTITVYSVVT
                     PGEFAPPGPQCPALLCLPIGISGTVLQWGHPLSGVTAISTPYDDAACTIPFATNYNKQ
                     GLTNGAGIYMIPGGPGLATLGWGPNISWLIENNSTSPGTFWFSVSADGAVSDCHPVTV
                     PGAPPPPVSPTAVPGQTYLSYSRYPISTPGLVLLSQADSLVAAGLSAVGSHGATPCAD
                     TTGVNCEGYTVPFAISPALAFLRGVPGPWTMMGGYVLVSNVQGLTYQNEAVDGSGMVQ
                     SQAQVDAFEAYYGQFGKDYNNNAPNLWKIYG"
     gene            complement(5220..>6128)
                     /locus_tag="VEH48_00040"
     CDS             complement(5220..>6128)
                     /locus_tag="VEH48_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXY17803.1"
                     /translation="SNARRVLLYVHINDIAGNPKKSLPLPMMNVLNGGKHASNSSDFQ
                     EFMIIPQRAGSYAQAIQIGSEVFHSLKNEIASAGASTAVGDEGGFTYGVKANTEMFDL
                     LSKATEAAGYKPGDDIGYGLDVAASELYNGGSYVLKAEDRQLNSAEMIDYLAAISQKY
                     PLLSIEDGLAQDEWDAWVELTKRLGQLQLVGDDLLVTNPVRLQRAIDLQAGNAILIKP
                     NQIGTLTETIRTLQLAKSHGWRTVISHRSGETEDVTIAHLAIGTGADQIKTGSLSRSE
                     RTAKHNEIMRIEAIDETLQLANHLKT"
BASE COUNT         1377 a         1780 c         1696 g         1275 t
ORIGIN      
        1 cagtacgcgc ctttaagtgt ctggcagcag gtcgccagcg tgttttcggc caccgaaggc
       61 gcctttgaca acgtaccaat agagaatctc aaggccgccc aggacgccat gttggctgac
      121 attgaacaaa accacgaacc ggtaattact gagctcagca aaggcgacaa accgtccgag
      181 gccagcaaaa aagctattct ggccgccgcc gccaaaattg ccaagcaata tcgtaaagtc
      241 gaactgaaag acgaggctga aagctgatgg cccagacccg cgtcttgcaa cgccgtatcc
      301 gctcggtgag aaacgccaag caaataacca aagctctaga ggtggtagcc gccagccgga
      361 tgcgcaaggt tatacagctg gtcgccggca gccgcagtta tggcgagctg gcggcaggca
      421 tcatgcggca tatcgccgcc aatcccgaag ccgccgcgca cccctacttc aatcctgaag
      481 ccggcaaacc gacgctgtac gttatcatca actccgaccg cgggcaagcc ggcgccttca
      541 atgccaacat cttcagcctg gccatcactt cattcaaagc cgaaaaaatc cgcccccaag
      601 tggtggtttt tggccgcaaa ggcacacgct actttgccca cctggccgga attgacctgc
      661 gtggtgccta cgaagacatc ggcgatatgc cggacgtcaa tgtcttcgcg ccggtaatgg
      721 aaatgatcca aaccgaacta gcgggcggtg aaatcggccg ggtggtaatc ctctacacgc
      781 agtttctgtc atcgctaagc caaaaagcta cctctttcca actgctgccg atcgacttga
      841 gcgctgccgg cgacgccgaa cctgccaaag tctacgaatt tgaaccggaa gtcaaggatg
      901 tactggccga agccattcgt ctttacgccg aagctaagtt gatgcaggct aagattgaat
      961 cggccgccag cgagcaagcc atgcggatgg tcgccatggg caacgccaac cgcaacgcca
     1021 gcgatttgat tgataacctg acgttggaac tgaatgccac ccgccaggcg gccatcaccc
     1081 aagagatcgc tgaaataacc ggtggcgccg cggctatcgc acaatagagt aataagcgga
     1141 cagtagtaag aagtaagggg aaacagatgc cagaaacgac caagactaaa ccagcaacca
     1201 agaagggcgt tatcacccag atcgtgggcg tagtcataga cgtggagttt gaaggccacc
     1261 ttccctctat ttataacgct ttgactgttg atctggacgg taagactttg ctgatggaag
     1321 tcgcccagca cctcagccgc caggctgtac gagccgtcgc tttagggccg accgacggat
     1381 tggcgcgtgg tactacggtt actgacaccg gcgcgcctat cagcgtgccg atcggcgagc
     1441 cgacgctggg ccggatgttt aatgtgatcg gcgagccgat tgatggcaag ccagtgccag
     1501 ccaaagctgt actcgggcct attcaccgcc aggcgccgcc gctgaccgac caaaacccca
     1561 aagtcgaaat cctggaaacc ggcatcaaag tcattgacct catctgcccg atcaccaaag
     1621 gcggcaaggt cggcctgttc ggcggcgccg gtgtcggcaa gaccattttt atcaccgagc
     1681 tgataaacaa tatcgccaaa ttccacaaag gccgcagcgt gttcgctggc gtcggcgagc
     1741 gtacccgcga aggcaacgac ctctactttg agatgcagaa ctccaaagta ttagacaaaa
     1801 cctcgttggt cttcggccag atgaacgaac cgccaggcgc ccgcttgcgc gtcggcctga
     1861 ccggtctggc tatcgccgag agtttccgcg acgctggcaa cgacgttttg ctgttcatag
     1921 ataatatttt ccgcttcacc caggccggtt ccgaagtatc agccctgctg ggccgtctgc
     1981 ccagcgccgt aggctaccag cccaacctgg ctaccgagat gggcaccctc caagagcgca
     2041 tcacttccac cactaaaggt tctatcacct ccgttcaagc ggtgttcgta ccggccgatg
     2101 acctcaccga cccggcgccg gctaccactt tcgcccatct ggactctacg gtcgtcctat
     2161 cgcgtgccct gaccgagctt ggcctctacc cggcggttga cccgctggat tctacatcca
     2221 caattttgga tccggaaacc gtcggccagg agcactacga ggtggcccgc gaggtccagc
     2281 gggtactgca gcgctataag gacctgcagg acatcatcgc tatcctgggc atggaagaac
     2341 tgtccgagga agacaaaaag ctggtggccc gcgcccggcg catccagcgt tttttgaccc
     2401 agcccttctt tgtcggcgaa gtcttcacta acattcccgg tgtttatgtg ccgctggccg
     2461 agaccatcaa aggcttcaaa gaaattttag acggcaagca cgatagcaaa aacgagcaag
     2521 ccttctatat gaaaggcgcc attgaccaag tcaaagacga caagcaaagc agcaagcaac
     2581 cagaaacaag cgataaggaa atagccagca atgataagca gccggaggag acgccgtgat
     2641 gcgttttcaa ctggtggcgt catccggcct taaatttgac gatgaagctt acgaggtcat
     2701 ggtgcccacc aaagccggcg tcatcagcat ttttgaagac cacatgccac tgatttcgtc
     2761 ggcccagccg ggagttttaa gcatacgcaa aaaagctggc gacaacgata gttctctgga
     2821 gcacttcgcc atcaacggcg gtatggtgga agttgacggc aaaaccgtca gttttgtagc
     2881 cgacgatgtc accaccgccg atgaaatcag cgaaaaagat gccgaagcgg ctttggcctt
     2941 cgccgaagat ctggttaaga atgctgatac ccaatcggcg ctgcacgaag cccaccgcac
     3001 gctgcagcac agcagcgccc agctgcacat cgcacgcctg aaacggcgcc accaccaata
     3061 aatttagaag gcgctggact ggctgttcag ttggttgaga tctgagctgt tatcgttggg
     3121 gttgtcggca ttcagggtgt tggcggcact attcaagtca gtggtgttat tgacggccgc
     3181 cggtaccgaa ctggggctgg aagtagaagt ggaagtggaa gatttgggca cggccacctg
     3241 ttgcgccgca ctctcgttgt taagtttatt gacattgttg cggtcgtggt aaaccgtgta
     3301 gcctacaaag ccgataatgg cgactattgc caaaatcaac aaggcctcta tggccgcgaa
     3361 cccgggattt actttggctt tactcattac tggctccctc cgccgcttat gctgctgggt
     3421 tcaacgaccg atttgacgcc ggctatcaat gcctggaccg ccgctttgta gttttgcagg
     3481 gcggtgattt cagcctgaac gtcggctttg aaagtctgta agttgccgac gggatcgctg
     3541 ccgctgcagt tgaagctggt actgtcggac tgcacctggc tgaccgccgc ctgggcggca
     3601 gttttatcgc tgttgaccgt agccaccagg ctgtcgtagt tatctaaagt cttgccgctt
     3661 ttgctgtaaa atgtttcaac tttagtggcc acatcgctga tcagcgtcag gtggcggttg
     3721 ccgcggtcgg caacccgctt taagatactg tcaatgacat ttacgcggtc ggtgcaaagt
     3781 ttgagtttgg cggcgtccag acgggtgccg gccgctacag cagccgggcc tgaagacgtg
     3841 gtagcgattg caaaggccgg gatggccacc accagcgtac tgccggcaat cgccaatgcc
     3901 ccgctaccag ccaggagttt tgcttttaaa gacgtcttta gcataaattc cttcttattt
     3961 aggttaatga agatacttct aaagcttatt atcgcagcat aaattagtca aatccaagat
     4021 ataaaaacag ccccgcgctt ggggcctctt ttttgagagc ccccatgggg cctggcggat
     4081 cgccaagccc catggggtag tgaacacgcg ggtgcgagtg aatccgcacc ccgcgtgtga
     4141 ccctctaccc gtagatcttc cacaggttgg gggcgttgtt gttgtagtcc ttgccgaact
     4201 gaccgtagta ggcctcgaag gcgtcgactt gtgcctggct ctgaaccatg ccagagccgt
     4261 cgactgcctc gttctgatac gtcagaccct ggacgttact gacgagtacg tagccaccca
     4321 tcatcgtcca cggaccggga acccctcgca ggaacgcgag agccggcgag atcgcgaagg
     4381 gaacggtgta cccctcgcag ttcactccag tcgtgtcggc acagggtgtc gcaccgtggg
     4441 aacccacggc cgatagccct gccgcaacga gagagtcggc ctgcgagagc agtacgagtc
     4501 ccggcgtcga gatcggatac cggctgtagc tgaggtacgt ttgccctgga accgccgtgg
     4561 gtgataccgg cggcggcggg gcgcccggca ctgtcaccgg gtggcagtcc gagactgcac
     4621 catcggcaga aacgctgaac cagaacgtcc ccgggctggt cgaattgttc tcgatcagcc
     4681 acgagatatt cggcccccag ccgagggtcg caagacccgg cccaccggga atcatataga
     4741 tccccgctcc gttcgtcagc ccctgcttgt tgtagttcgt ggcgaacgga atcgtgcatg
     4801 ccgcgtcgtc gtacggcgtg ctgattgctg tcacaccgga cagggggtgt ccccactgca
     4861 ggacggtgcc gctgatgccg atcggcaaac agagaagcgc cggacactgc ggcccaggag
     4921 gcgcgaactc cccgggcgtg acgacactgt agactgtgat ggtctgcgtg accgtcacgg
     4981 taccaccgcc atggtgccat ccgcctgcat tggaggccgt ggccgagaag gccatggccg
     5041 cggtcactgc ggcgaacaat gcgagcaatc gcttcgtaga cacgaaacga tcaccccctt
     5101 tcaggttgtt gatgtacgtt tcctcccaaa aaccaaacag acccctggga agctaaggca
     5161 tattgtacgc taaaaataca ctttagtcaa tgtgggcccc aagaactcct tataggtagt
     5221 tacgtcttta agtgattggc cagctgcaac gtttcgtcaa tggcctcaat acgcataatt
     5281 tcgttgtgtt tagctgtccg ttcgctgcgg ctcaaactgc cggtctttat ctggtccgcg
     5341 ccggtgccga tggccaggtg agcaatcgtc acatcttcgg tttcgccact tctgtgcgag
     5401 ataaccgtcc gccagccgtg gctttttgcc aactgcaagg tgcggattgt ctccgtcaac
     5461 gtgcctatct ggttgggctt gataagaatg gcgttgcccg cctgcaggtc gatggcccgc
     5521 tgcagtctga ccgggtttgt caccagcaag tcatcaccga caagctgcag ctgcccaagc
     5581 cgtttggtca gctccaccca ggcgtcccat tcatcctggg ccagcccgtc ttcaatcgac
     5641 agcaacggat atttttgcga aatagccgct aaataatcaa tcatttccgc gctgttcagc
     5701 tgccggtcct cggctttcaa gacatagctg ccgccgttat acagttccga cgccgcgaca
     5761 tccagcccgt agcctatgtc atcgcccggt ttgtagccgg cggcctccgt cgctttagat
     5821 aacaggtcaa acatctcggt attggctttg acgccgtagg tgaagccgcc ttcgtcgccg
     5881 acggccgtgg acgcgccggc cgaggcaatc tcattcttca gcgaatggaa tacctccgag
     5941 ccgatttgta ttgcctgggc ataactgccc gcgcgctgcg gaataatcat aaattcctga
     6001 aagtctgagg agttagaggc gtgtttgccg ccgttgagaa cgttcatcat cggcaacggc
     6061 agtgattttt tgggattgcc ggcgatgtca ttgatatgca cgtacagcaa cacccgccgg
     6121 gcgttgga
//
LOCUS       DASPTF010000002         7552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_723875, whole genome shotgun sequence.
ACCESSION   DASPTF010000002 DASPTF010000000
VERSION     DASPTF010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723875"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3296
                     /locus_tag="VEH48_00045"
     CDS             <1..3296
                     /locus_tag="VEH48_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17804.1"
                     /translation="SALTTIIASYSPAGGAPVSCTPTNQTVVTGGWTRYQCTLSSATA
                     PSTSGYIQITQGASDTTAHTFYIDGVQLEQASTASNYSDGTINLNGVISNPVVIQDGA
                     NSVNAFQVQNAAGNNFILADTTTATLSLGNTGIASTIQIGNTTGAVTQTIYIGNNATA
                     SSVTNVTIGSTIGASTTTIDSGTGNINLNPSGTSNTGVLVKPGANSTAAFQVQPAASA
                     TAVLAVDTTNNVVTISSGTSNVAGLKFGNLTSANAGGTQYTGILGLDTSGNIGLSQAS
                     VSLTSPALAYWDGLNDPTTSSQSYPLASVSGTGTGSVGSCSGTWAPAFVSGSGEELTP
                     ACTTTSGSINWSFSQVSFEETQFQFKAGGGTHADSTWFYSYADGVPTTEYGAGGAYTQ
                     GYLIYFSEWHHCVGIAWGPYTDGNQCGSGGGANPLTSASLWNIDDNNFHNVDIQLRYN
                     VITVRWDGNIVLNYTDTFGRTLSNQDFGFGSRTGGNTNIHYIKGLLVTKLGSDPSRYN
                     IDTVSPLASGLYWNNSNTRLGISTSSPDSTLEVDGTASYKTVVINGTGVSANNYVYQG
                     SGSAMPACTSTNESTGYTASTTVTGAGTSFPGFTSAMVGDTITLPDGVQDTITGYTNS
                     TTITVSGSRIECEGSYTVLTPALIVSGTTTPTISLGATGTSSTSSTTHIGDTSGSTGT
                     QAITIGSSANTANSLTLEAGTGTTALFNGATAHTIQIGTGAAIQTITIGSQNSTSDLT
                     LQGGVITTTNNQGGVDIGGGFSTSDTNLIPLVLDSTTTYSETANTCTATANDGALYFN
                     SNTSSMAIRACINGGWEDVVTTSGLGILTYGIVPDSGTTPGDLAGVNAADASKGPCHV
                     YMGSVANSVRWTGCTMYSQGRKQIIAAQATNYTTSITTTGSAYQNLCIFTAGSGPSFG
                     TASTTWTSATVPSFSPGSPAVCLATIKEISGGSGIGVIYDTRVFTDSTKAFVSLSTAV
                     SPGFAVKLNGTIGQSVPTAATTDPFYGVVAIWSGTTQTTAINGVVVTHGPIYVTASAG
                     SVGAYVKPTATSGVVNTSALVTTAQTDIPYTYLGLAQSPYNAPGTQCSTTANADTCRG
                     SILVDVNIR"
     gene            3339..3827
                     /locus_tag="VEH48_00050"
     CDS             3339..3827
                     /locus_tag="VEH48_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17805.1"
                     /translation="MKRLWYLLTGLLLLIGVIACVIWLWPKPLIPKDIQNKVTSVIFV
                     PKNPQVINKKGTVKYDSDLKLLSYQATIFGVNTVISEQPTPESFTDIPQVYQKVLDSW
                     NQYETFNTPQGTVYLTRPGKQSNEEVGVMNSKGTFLFIKPDSNFTDSQWRQLFNSLQT
                     DS"
     gene            complement(3875..4429)
                     /locus_tag="VEH48_00055"
     CDS             complement(3875..4429)
                     /locus_tag="VEH48_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17806.1"
                     /translation="MPQSPLLIGMLIGVSAAAAGWLAFGGTNSNKTAPPKPQAQSSPV
                     SQKKTVSTNNTSTLGASTNPASKPAPTPSYTSSLPTGFYLNNDAQNLGNGVITYSIND
                     SNGNTYSVVQEPLPANSNATVFAQKLTDTETFTAPVGNAVIGDSGSELLAGVNAPNNT
                     WIIIEAPNTSLRPQLEELVRSLRS"
     gene            complement(4613..5419)
                     /locus_tag="VEH48_00060"
     CDS             complement(4613..5419)
                     /locus_tag="VEH48_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17807.1"
                     /translation="MDQDFRGGLAAGQPAGAQPYRPPAPVHQPALQQPLTPPQPVVPA
                     APAAPAVQTQPAAQSQPAAFFPPAAPAAQPTPQQPPQPAQPFAPQPAPAFAQPEETKK
                     SGKFKPAAVSLAVVVLAVGGFTVIDHKINSNSSALSGYFPPSVSQSKLSIPAYYPVNL
                     PAGFSVGGYKVINQKMLNYVVANKDKDQFYVSVQPLPSTAELTAFSKKVSNQTLADTP
                     AGSGLVGTSGDTLVGSVQTTKNVWILISSTAISKAADLETIIHSFEQVNK"
     gene            complement(5462..5896)
                     /locus_tag="VEH48_00065"
     CDS             complement(5462..5896)
                     /locus_tag="VEH48_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17808.1"
                     /translation="MMVFAAAGTVYVFISDRDSKNTSPAIYNVPAEQSPLPKAVAPSP
                     NAPEGVAIETLVSPVAAGDNSTVSARTNAGSSCTISVTYNGIAATDSGLAPQKADAYG
                     SIQWSWTIPSSTPPGNWPIKIICVYNGRSGVVDGSLQVTKSQ"
     gene            6163..6238
                     /locus_tag="VEH48_00070"
     tRNA            6163..6238
                     /locus_tag="VEH48_00070"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6196..6198,aa:Asp,seq:gtc)
     gene            6470..7249
                     /locus_tag="VEH48_00075"
     CDS             6470..7249
                     /locus_tag="VEH48_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G1 family glutamic endopeptidase"
                     /protein_id="HXY17809.1"
                     /translation="MNTNTIFPNKLRHFKTSWLAAVLAAAYVAALALGLAFSSRAASL
                     PNHMPFRRLLHSTSSNWSGYAAYGAAGSFNSVSANWVQPLVTCSNRQNSYSSFWVGLD
                     GYSDSTVEQIGTEADCSRGHAQYYAWYEMYPHSTVLITTALKAGDSLSASVVYSAKNT
                     FSLKLSDSTSHLTYSTTQRMTSPQLSSAEAIAEAPWSRGVLPLANFGTANFSAALANG
                     QPLGGFTSLDPLTMIDPYGMKSTPTAFDSTKESFSVTWSAS"
     gene            7303..>7552
                     /locus_tag="VEH48_00080"
     CDS             7303..>7552
                     /locus_tag="VEH48_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17810.1"
                     /translation="MKASNTPGSDHESKDSLGPPRPDVVKSFVLSDIIIAPVSPDESD
                     FLRPVTELKKISRQLGGDEHVQPHHIGGGYLQLSISERA"
BASE COUNT         1785 a         2088 c         1856 g         1823 t
ORIGIN      
        1 ccagcgcgct taccaccatt attgccagtt actcgccggc cggcggtgcg cccgtaagct
       61 gtactcctac caatcaaacg gtagttaccg gcggctggac gcgctatcaa tgtacgctgt
      121 ccagcgcgac cgctcccagc acatccggct atatccagat tacccaaggc gcttcagata
      181 ccacggccca tactttctat attgacgggg ttcagcttga gcaggcaagt accgccagca
      241 actacagcga cggcacaatc aaccttaatg gggttatcag taatccggta gttatccagg
      301 acggcgccaa ttcggttaat gccttccagg tgcaaaatgc cgccggtaac aacttcatac
      361 tggctgacac gactaccgcc accctttctc ttggcaatac cggcatagcc agcactatcc
      421 aaattggtaa caccaccggc gctgtaaccc agacgattta cattggtaac aacgctacgg
      481 ccagttcggt aaccaatgtg accatcggct caactattgg cgccagcact accacaattg
      541 actccggtac aggcaacatt aatcttaatc cttcaggcac cagcaacacc ggcgtgttgg
      601 taaaaccggg cgccaactct acggccgcct tccaagtcca gccggccgct tccgcaactg
      661 cggtgctggc cgttgacacc actaacaatg tagtaacgat ctcctccggc accagcaatg
      721 ttgccggcct gaaatttggg aacctgactt ccgccaatgc cggcggcaca caatatacgg
      781 gtattttggg tcttgataca tcaggcaaca ttggtctatc gcaggcttcc gtatctttga
      841 cctcgccagc tttagcttac tgggacggtt taaatgatcc cacgacaagc agtcaatcct
      901 atccgctagc tagcgtatca ggcactggaa caggttccgt tggttcttgc tccggaacct
      961 gggcaccggc ctttgtcagc ggtagcggcg aagagttgac tccggcttgt accaccacct
     1021 cgggttcaat aaactggagc ttttcccagg tttcctttga ggagactcag ttccagttca
     1081 aagccggcgg cggtacacat gccgactcca cctggttcta tagctacgct gacggagtac
     1141 ctaccacaga atacggcgcc ggcggagcct acactcaggg ttatctaatc tacttcagcg
     1201 aatggcatca ctgtgtcggc attgcctggg gcccctatac cgacggcaac cagtgtggtt
     1261 ctggcggcgg cgctaacccg ttaacttcag cgtcactttg gaatattgac gacaacaact
     1321 tccacaacgt tgatatccag cttcgctaca acgtcatcac tgtccgctgg gatggcaata
     1381 tcgtcctgaa ttacacggac acatttggcc gcaccctttc caatcaggat ttcggctttg
     1441 gctcgcgcac cggcggcaat actaacatcc actatatcaa gggcttgctg gtaaccaagc
     1501 tcggttctga tccatccaga tataatattg ataccgtatc gccgctggcc agcggtcttt
     1561 attggaacaa ctcaaacacc cgtcttggta tcagtacttc ttcaccagac tccaccttgg
     1621 aagttgacgg tacggcttct tataagacag tcgttatcaa cggtactggc gtcagcgcca
     1681 ataactatgt ttatcaaggc agcggctccg ctatgcctgc gtgtacctca accaatgaat
     1741 ctaccggcta tacagccagt actaccgtta ccggcgctgg cacatcgttc ccggggttca
     1801 cctctgccat ggttggcgat accataacgc tgccggacgg cgtacaggat acaattacag
     1861 gctataccaa cagcacgaca attactgttt ctggctcgcg aatagagtgc gaaggatcat
     1921 atacagtgct tacccctgct ctaattgtct ctggaacaac gacgccaaca attagcctag
     1981 gcgctacggg tacctcttca accagctcga ccacccatat aggcgatacc tcgggcagca
     2041 ccgggaccca agctatcaca attggctcgt cagctaacac tgctaacagc ttgactttag
     2101 aagccggcac cggcaccacg gctttgttca acggcgccac cgcccacacc atccaaattg
     2161 gtaccggtgc cgctattcaa actataacca tcggttcgca aaactctacc agcgatttga
     2221 ccctgcaggg cggcgtaata accaccacca acaaccaggg cggcgtagac attggcggcg
     2281 gcttttccac ctctgacacc aatcttatac ctttggtcct agactcaaca acgacctata
     2341 gcgagacggc caatacctgt acggcaacgg ctaatgacgg agccttgtac tttaactcca
     2401 acacctcctc tatggctatc cgggcttgta taaatggagg ttgggaagat gttgttacaa
     2461 ccagcggtct gggcatactc acctatggta tagtgccaga ttccggcaca acgcccggcg
     2521 acttagccgg cgtaaatgct gctgatgcct caaagggacc atgccacgtc tatatgggta
     2581 gcgtcgctaa ttctgttcgc tggactggct gcactatgta tagccaaggc cgtaaacaaa
     2641 ttattgcggc gcaggccacg aactacacta caagcattac taccacagga agtgcttatc
     2701 aaaatctctg tatatttacg gccggaagtg ggccgagctt tggtaccgcc agcactactt
     2761 ggacgtcagc taccgtgccg tctttctcgc cagggagccc agccgtttgc ctagcgacaa
     2821 ttaaagaaat ctccggaggc agcggcatag gagttatcta tgatacccgt gtatttacag
     2881 attccactaa agcattcgtt tcactttcta cagccgttag ccccggcttc gcggttaaac
     2941 ttaatggcac gattggacag tcagttccta cggctgccac caccgacccg ttctacggtg
     3001 tggtagctat ctggagcggc accacgcaaa ccactgccat caacggcgta gtcgtcaccc
     3061 atggaccgat ctatgtaaca gcttcagctg gatcggttgg tgcctatgtc aagccgaccg
     3121 ctacctccgg cgtggtaaat acatctgcgc ttgttacaac ggcgcagacg gatataccgt
     3181 atacctacct cggtttagct caatcaccat ataacgcccc cggtacgcaa tgcagtacta
     3241 cagctaatgc cgacacctgc cgtggctcaa tattggtaga tgttaacatt agataatctg
     3301 gttccataaa tgttgccagg gaatggtatg gtattgtcat gaaacgctta tggtacttac
     3361 taacgggtct gttgctgctt ataggagtta ttgcgtgtgt gatatggctt tggcccaaac
     3421 cgctaatacc aaaggacatt caaaataaag tgacgtctgt catttttgta cccaaaaatc
     3481 ctcaagtgat aaacaaaaag gggacggtta aatatgatag tgatctaaag cttttaagtt
     3541 accaggcaac aatcttcggt gtgaacactg taattagcga acagccgacg cccgaaagct
     3601 ttacagatat tccgcaggtc tatcagaaag tgctggatag ttggaatcaa tacgaaacgt
     3661 ttaatacgcc gcagggaact gtatacctga ctcgcccagg caaacaaagt aatgaagaag
     3721 tgggagtaat gaattcaaaa ggcacctttc tatttattaa gccggacagt aattttaccg
     3781 attcccaatg gcgccaacta tttaactccc tccaaaccga cagctaaacc gcaaccagcc
     3841 ggccaaattt gttatatcta tatcctgttt taacttagct tctcaaactt cggaccagct
     3901 cctctagctg cgggcgtaag cttgtgtttg gagcttcaat tataatccag gtgttattcg
     3961 gagcgtttac gccagccaga agctcagaac cactatcacc aatcaccgca ttgcccaccg
     4021 gagcagtgaa tgtttccgta tcagtgagtt tctgggcaaa aaccgtggcg ttggaattgg
     4081 ccggcaaagg ttcctgcact acagaataag tgttgccgtt actgtcattt attgagtagg
     4141 ttataacccc gttacccagg ttttgtgcat cattattaag gtagaaacca gtaggcaggg
     4201 agctggtgta gctgggcgta ggagcaggct tggatgctgg attggtgctt gcgcccagag
     4261 ttgaagtatt attggttgaa acggttttct tctggctgac gggggaggat tgagcctgcg
     4321 gtttcggagg ggctgtttta tttgaattgg tgccgccaaa cgccagccag ccggcagcag
     4381 ccgcggatac accaatcaac atacctatta ataaaggtga ttgcggcatt ttttttaaaa
     4441 acttaaagcg taagcttcct aaacccaatg gctttgcatc tgcgcgataa tcagatgctc
     4501 gcggcgccga actagcagta tttacttgtt tgttcagacg tgcgggcccg attctttcag
     4561 ctgtaaagtt gcgtctgtag cggtttctta agttatcatc ggtcattagg gtttatttat
     4621 tcacttgttc aaaggaatgg atgatcgttt ccaggtcggc ggctttgctg atagccgtag
     4681 aactgatcag tatccagaca ttcttagtag tctggacgct gccaaccaga gtatcgccgg
     4741 acgtcccgac cagcccgctg cctgccgggg tgtcagccag tgtctgattg ctcactttct
     4801 tactaaaagc cgttagttca gccgttgaag gcagcggttg tacgctcacg taaaattggt
     4861 ctttatcctt gttagcgacg acataattaa gcattttctg gttgatgact ttatagccgc
     4921 cgacgctgaa accggccggc aggttcaccg gataataagc cgggatgcta agctttgatt
     4981 ggctgacgct tggcgggaag taaccggaca gcgccgaact attagagttg atcttatggt
     5041 caattaccgt aaaaccgccg actgctagaa ccactaccgc caaagaaacg gccgccggtt
     5101 tgaacttacc gcttttttta gtttcttcgg gctgggcgaa cgcaggtgcc ggttggggcg
     5161 cgaacggctg ggccggctgt ggcggttgct gcggggtagg ctgcgcggcg ggcgccgctg
     5221 gcgggaaaaa cgccgccggc tgtgattgtg cggctggctg cgtctgcaca gccggcgcgg
     5281 caggcgccgc tggcacaact ggctgaggcg gcgttaacgg ctgttgcagg gctggctggt
     5341 gcactggtgc cggcggccga tatggctggg cgcccgccgg ctggccggct gccaaaccgc
     5401 ccctaaaatc ttggtccatt acttatataa gatatagcat tcctgtcttt tggcacaagc
     5461 tttactggct tttagtcacc tgtaaactgc catcgaccac accgctgcgg ccgttataca
     5521 cacagatgat tttaattggc caattgccgg gaggtgtaga actaggaatc gtccacgacc
     5581 actgtatact gccgtaagcg tcggcttttt ggggcgccaa accggaatca gtagcggcaa
     5641 tgccgttata agtgactgat atggtacagc ttgagccggc gttggtcctg gccgaaactg
     5701 tagaattgtc gccggcggcc accggtgata ccaaagtctc tatggccacg ccttccggcg
     5761 cgttcggcga tggcgccaca gccttgggca gcggcgactg ctcggcaggc acattataaa
     5821 tcgccggcga tgtatttttg ctgtcgcggt cgctgataaa cacgtaaacc gtcccggctg
     5881 ctgcaaaaac catcatcatc acgactacag tgataccgat ttttttagta gttctaagga
     5941 tgttctctcc tatatgtttt agcggatttc taactattta agatacacta tccgccgctt
     6001 tattttgact cccgtatctg ttagaataca tctgttcttt tcttgtttta ttacaaaaac
     6061 gcgagatctg tccgagtcgc gtttttgaga ggaagaggcg ggccaaggcc ggaagtaagt
     6121 cacacaagtt acttacggag gccggcgtcc cgactctgac gaggggcggt ggagcagcgg
     6181 agtgctcgtc tccctgtcac ggagaaggtc gcgggttcaa atcccgtccg ccccgccaac
     6241 aaaaatgtct caccgctagg tggggctttt ttgttggcgg tagttgggcg cggcgatttg
     6301 aacccgcggc gggttagccc cgcgcggcga tcgggaggtc ggaaagccgg agcgctatcc
     6361 cgtccgcccc gccaagtata tagcttttat agtttaaaaa cgggggtaaa aaacaaaaat
     6421 tccaccactt gacgaagtgt tttataaagc gtaaagtaat ttttatatta tgaataccaa
     6481 taccattttc cccaataaac tccgccattt taaaactagt tggctggcgg cagttctggc
     6541 cgctgcctac gtcgccgccc tggctcttgg cctggctttc agctcccggg ccgccagcct
     6601 gcccaaccac atgccattcc gccgcctgct gcacagcacc agctccaact ggtccggcta
     6661 cgccgcttat ggcgccgccg gcagttttaa cagcgtttcg gccaactggg ttcagccgct
     6721 ggtaacctgt tccaaccgcc aaaacagtta ttcatctttc tgggtcggcc tggacggcta
     6781 cagcgactcc acagtagagc aaatcggcac cgaagccgac tgcagccgcg gccacgccca
     6841 atactatgcc tggtacgaaa tgtacccgca ttccactgtt ctgatcacca cggccctgaa
     6901 agccggcgac agcctcagcg cttcagtggt ttacagcgcc aaaaatactt tttctttgaa
     6961 acttagcgat agcaccagcc acctcaccta ctccaccacc caaaggatga cctcgcccca
     7021 actaagctcg gctgaggcta tcgccgaggc gccgtggagc aggggagtac tgccgctggc
     7081 caacttcggc accgccaact ttagcgccgc tctggccaac ggccagccgc tgggcggttt
     7141 taccagcctt gatccgctga ctatgattga cccctacggc atgaaatcca cgcccacagc
     7201 ctttgacagc accaaagaaa gcttctcggt cacttggtcc gccagctaaa ccaaattcgc
     7261 cacttcaatt tccgctaagg tctctgctac aatggctttt tagtgaaagc ctccaatacg
     7321 ccgggctccg accatgagtc caaagattct cttggtcctc cgcgccccga tgttgttaaa
     7381 tcgtttgtat tatcagatat aataattgcg cctgtttctc ctgacgaaag cgattttctg
     7441 agacccgtta ctgaacttaa aaaaatatcc aggcaactag gtggagacga gcatgtccag
     7501 cctcatcaca tcggtggcgg ctaccttcag ttatcaataa gcgagagagc tg
//
LOCUS       DASPTF010000003         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1441303, whole genome shotgun sequence.
ACCESSION   DASPTF010000003 DASPTF010000000
VERSION     DASPTF010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441303"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..162
                     /locus_tag="VEH48_00085"
     CDS             40..162
                     /locus_tag="VEH48_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17811.1"
                     /translation="MAFTPSEEILDKYAKVLVNFALGGSKGIKPGEVVYLQSPL"
     gene            166..1251
                     /locus_tag="VEH48_00090"
     CDS             166..1251
                     /locus_tag="VEH48_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXY17812.1"
                     /translation="MPFYRALRKNILDSGGLAINNLADDMSGAAKYFYDNANQEQLTF
                     FMDKYFRGLVDQVDHRIAVLADYNVHEMDKVDPKKMMLVQKTAKPLIQWFDKKEHDGR
                     YTWTLALYGTPSMAKEAGLSEKEYWDQIIKACYLDYADPIAQWRKVSKEVQRVANKLT
                     ELNIQKVRIKGPDVDLHIAIGEKRKWLAGGGRNIPSFEVFTSPDWRGTDGWIRFNVPL
                     YYYGPKVEGIELEFKSGRVLKATAKKNQKLLREMLATDTGAAQLGEFSLTDKRISRIT
                     KFMADTLFDENIGGPYGNTHVAVGRSYLDTFQGNAAKMTKSQKRHLGYNESVIHVDMF
                     STTKRTVTATLSDGSQKIIYKDGQFTI"
     gene            1268..1531
                     /locus_tag="VEH48_00095"
     CDS             1268..1531
                     /locus_tag="VEH48_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17813.1"
                     /translation="MAKRPTNQEPDALYILKIVLYLIVGSQWLKIQTKTGSQIPIPYG
                     ALVGLLFALHDHFQIDRKVEYAVLLVAMFIGFWVPIGLVLTFH"
     gene            2033..>2238
                     /locus_tag="VEH48_00100"
     CDS             2033..>2238
                     /locus_tag="VEH48_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17814.1"
                     /translation="MKALITRKLGMTSVISEDGALLPVTLLSASPNVVSQIKSADKDG
                     YFAIQLGFETAKKPAKPQTGHFKGL"
BASE COUNT          585 a          568 c          560 g          525 t
ORIGIN      
        1 aacaacaaaa agagcaaact atccctataa ttgatgccaa tggctttcac acctagcgag
       61 gagattctgg ataaatatgc caaggttctg gtcaatttcg ccttgggcgg tagcaagggc
      121 atcaagcccg gcgaggttgt atatctgcag tcgccgcttt gagcgctgcc gttttaccgc
      181 gcgctgcgca agaacatctt agatagcggc ggcctggcta tcaacaattt agccgatgat
      241 atgtcaggtg ccgccaaata cttttatgat aacgccaatc aggaacaatt gactttcttc
      301 atggacaaat attttagagg cctggtagac caagttgacc accgaattgc agttttggcc
      361 gactacaacg tgcacgagat ggataaagtc gaccctaaaa aaatgatgtt ggtacaaaag
      421 actgccaaac cgcttatcca atggtttgat aagaaagagc acgatggccg ttacacctgg
      481 acgttagctc tttatggcac gcccagtatg gccaaagaag ccggcttgag cgagaaagaa
      541 tattgggacc agatcataaa agcctgttat ctggattacg ccgacccgat tgcccagtgg
      601 cgtaaagtca gcaaagaggt ccagcgggtg gccaataaac ttaccgaact gaacatccag
      661 aaggtgcgca tcaagggtcc ggacgttgac ctgcacattg ccatcggcga aaaacgcaag
      721 tggctggccg gcggcggacg caacatcccc agttttgagg ttttcacctc tccggattgg
      781 cgcggcaccg acggctggat ccgctttaac gtgccactat attattacgg gccgaaagtt
      841 gagggtatag aattggaatt taagagcggc cgagtgctca aagctaccgc caaaaagaac
      901 cagaaactgc tgcgggaaat gctggctacg gataccggcg ccgcccagct gggcgagttc
      961 tcgctgaccg ataaacgcat cagccgtatc actaaattca tggccgatac tctgtttgac
     1021 gagaacatcg gcggcccgta cggtaacacc catgttgccg ttggccggag ttatctggac
     1081 accttccaag gcaacgcggc aaaaatgacc aaatcccaga aaagacactt aggctacaac
     1141 gaatcggtca tccacgtgga tatgttctct accaccaagc gcacagtcac cgcaactttg
     1201 tcggatggca gccaaaaaat tatctacaaa gacggccaat tcacgatttg atattatatt
     1261 agcgcttatg gcgaagagac caaccaatca ggaacccgat gctctctaca tcctaaaaat
     1321 cgtgctatat ctgattgtcg gttcgcagtg gctgaaaata cagaccaaga ccggttcgca
     1381 aatccctata ccctacggcg cgctggtcgg gcttctgttc gccctgcacg accactttca
     1441 gattgaccgc aaagttgagt acgccgttct gctggtcgcg atgtttattg gtttctgggt
     1501 gcccatcggc ctggttctga ccttccacta aaccaagaat tttttctacg gccttccgca
     1561 ccatttccct aaagaacccg ttccgcgcgg ggcactcagg tatggtgctc cggctgctac
     1621 ttaagcggct cacgttcttt cggtgaatgg tgctcgcggc ccttatatat gaactttttt
     1681 ctcgggactt gagccaaaca gactagttcg ggcctctccc gcagacctga ggtggatgat
     1741 gtggaaaaca ggcgtgcttg gccgctaaag catgtttgac gaccccgcac ctaagggctt
     1801 aggtgcgggg gaggagctct ttagcgagcc tgtttttgac tatcagacac ctcaaggcaa
     1861 gggagccggc ccgagagttt tggttacttt ggcggtccaa agtaacagaa gtagtttata
     1921 caacagtttg acagaggttt gttcggtgtg ttaatctgtt agtaagagtt gggcttggtg
     1981 ctcatattct gaatccctat gcttttagta aaacaaccat taaggactaa gcgtgaaggc
     2041 attaattacc cgaaaactcg gcatgaccag cgtcatcagc gaagatggcg cactgctgcc
     2101 tgtgaccttg ctttccgcca gtcccaacgt tgtctcacaa atcaaatcgg ccgataaaga
     2161 cggttatttt gcaattcaat taggctttga aacggccaaa aagccggcca agcctcagac
     2221 cggccacttt aagggcct
//
LOCUS       DASPTF010000004         3543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_578914, whole genome shotgun sequence.
ACCESSION   DASPTF010000004 DASPTF010000000
VERSION     DASPTF010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578914"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..664)
                     /locus_tag="VEH48_00105"
     CDS             complement(5..664)
                     /locus_tag="VEH48_00105"
                     /inference="COORDINATES: protein motif:HMM:NF020022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXY17815.1"
                     /translation="MNKTALNKPVLICLYGFPGAGKSYIARNLTGALQTALISADRIR
                     MELFERPRFDAQENAVINHLMNYMADEFLAAGISAIYDTNLIRTAQRHKLRELARKHK
                     AGFLLLWIQIDAESAFARTQERDRRTSDDRYAQPQTRTSFDQYLAGMQNPQDEDYLVL
                     SGKHSFITQKAAILNRLYQMGLVDSGSLQGTMTKPGLINLVPNPQAGRVDLSRRNINI
                     S"
     gene            complement(949..1023)
                     /locus_tag="VEH48_00110"
     tRNA            complement(949..1023)
                     /locus_tag="VEH48_00110"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(988..990),aa:Trp,seq:cca)
     gene            1209..2411
                     /locus_tag="VEH48_00115"
     CDS             1209..2411
                     /locus_tag="VEH48_00115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXY17816.1"
                     /translation="MRVLMLGWELPPNNSGGLGVVCLQLCSALAKAGADIDFVLPYYH
                     EPRYDFMRVISAPRRAGQEVFTFGNYDSRRYLLDDGSFVDYSLQDEQSAYTHMVGRLA
                     EYLEFDVIHAHDWLTFRAALLAKQKKNRPLILHVHSIERDRAGGQPGNPWVREIEATA
                     MLLADRVVAVSQRTKNMIVSDYHIPADKIDVVHNSLDLSDMAPLDEQNAYHYLTDLKD
                     DGWKVVTNVGRLTIQKGLPHLISAAGEVVKRFPKVIFLIVGHGEQRDELLELAAEKNI
                     SRNVLFAGFQRGKQWRDAYAISDLFVMPSVSEPFGLTPYEAIAYGSPSLISRQSGVAE
                     VFSNCLKVDFWDENEMANKIVAVLRSGPLRDELRSNAQEEYVKLSWSDAAERIMDHYR
                     QFTQRVAV"
     gene            2408..>3543
                     /locus_tag="VEH48_00120"
     CDS             2408..>3543
                     /locus_tag="VEH48_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HXY17817.1"
                     /translation="MKSIVFYLHAHQPWRLRHYTIFDISQGHNYFDTDPAHDNDNQRV
                     LQKVAQKSYGPTNAKLKMLLDKYPDFKLNISITGTLLDQLERWAPDVLASFQELAATG
                     RVEIATETYYHTLAFFYDREEFDRQVKQHKDKIEQLFGQTPVVFRNTELAYNNELAQW
                     AEAAGFKGILAEGWDPVLGWRSPNFMYRPAGTHSIKLLLKNYRISDDIAFRFGDSNWQ
                     EWPLTSEKFSHWLDQTHPAEMFNLFMDYETFGEHQWEDKGIFDFLEYLPEKWLSQDGN
                     TFMTFTEAIDKYEPKDEVDMPYTVTWADTERDLTAWVGNRMQQQAINAVYSLADKVAH
                     CGDQKLIDDWRRLQTSDHFYYMCTKWFNDGDVHAYFSPYESPYEA"
BASE COUNT          842 a          953 c          935 g          813 t
ORIGIN      
        1 ggcattagct gatgtttatg ttgcggcggc ttaagtctac ccggccggcc tgaggattcg
       61 gcaccaggtt aataagaccg ggtttggtca tcgttccctg cagcgaaccg ctgtcgacca
      121 gacccatctg atagaggcga ttcaagatag cggccttctg ggtgatgaac gaatgtttgc
      181 cgcttagcac caagtaatct tcatcttggg gattttgcat gccggccagg tattggtcga
      241 agctggtgcg cgtctgcggt tgggcgtagc ggtcatcgct ggtgcggcgg tcccgttcct
      301 gggtgcgggc aaaggcgctt tcggcgtcta tctgtatcca taacaataag aaaccggctt
      361 tgtgctttct ggccagttcg cgcaatttgt gccgttgagc cgttcggata aggttggtgt
      421 cataaatagc gctgattccg gcggccaaaa actcatcggc catgtaattc atcaagtggt
      481 tgataacagc gttctcctgg gcgtcaaagc gcggtcgttc aaacagttcc atcctgatac
      541 ggtcggcgct aataagggcg gtttgcaatg cgccagtcag gttgcgggca atataacttt
      601 taccggcacc ggggaaaccg tacaggcata tcaggaccgg tttatttaag gcagtcttat
      661 tcactcacta gatactacca ctctgttagc ccgccataaa gtgcctaagc taatattttg
      721 acatcttttg gggtccccat tacgcggggc tacgacctcc gggttcccat tatgctgagc
      781 tggaaatgta atttgtgggc tgttcccgtt ccgcgcggag ctgtggtcac ggggccccca
      841 ttacgcggag ctgcgtgatg gggtggagat ttgagccaac cgtaaatgag aaagcgacag
      901 ctatcgttta gtagctgtcg tttttgaata aaaggttgtg ctcaaatctg gcaggggcac
      961 gtggaatcga accacgatct agggttttgg agacccttat actagccgtt gtactatgcc
     1021 cctaagactt caaactcccc ccttatatta aattatgacg ggcaaaaaga ctacttctga
     1081 acaggttagc gtatctgtaa ctaaaaaaat tcaatatttc gcttgtgctt aagctgttaa
     1141 ggccttataa tcaaacctga tagcaaacag acagataaaa attaaaaaag aagggctata
     1201 agggacgtat gcgggtactc atgctaggct gggagctgcc gcccaacaac agcggcggcc
     1261 tcggggtagt ctgcttgcag ctgtgcagcg ccctcgccaa agccggtgcc gatattgatt
     1321 tcgtcctgcc ttattatcac gagccgcgct acgactttat gagggtgatt tcggcgccca
     1381 gacgcgccgg acaagaagtt tttacattcg gtaactatga cagccgccgt tatcttctgg
     1441 acgatggctc tttcgttgat tatagtctgc aagacgagca gtcagcctac acccatatgg
     1501 tcggacggct ggcggagtac ctggaattcg atgttattca cgcccacgac tggctgactt
     1561 tccgggccgc cctgttggcc aagcagaaga aaaaccgccc gctgatcttg catgtccatt
     1621 ccattgagcg cgatcgggct ggcggacagc cgggcaaccc gtgggtacgg gaaatcgaag
     1681 ccaccgctat gctgttagcc gaccgggtag tggccgtcag ccagcgtact aaaaacatga
     1741 tcgtctccga ttaccatatc ccggccgaca aaatagatgt tgtccacaac agcttggacc
     1801 tatccgatat ggcgccgctt gacgagcaaa acgcttatca ctatcttact gacttgaagg
     1861 atgacggctg gaaagtcgtt accaacgtcg gccggctgac catccagaaa ggactgccgc
     1921 acctgatcag cgccgccggc gaagtcgtca aacgcttccc caaagttatt ttcctgatcg
     1981 tcggtcacgg cgagcagcgc gatgagctgc tagagctggc ggccgagaaa aacatctcac
     2041 ggaacgtact gtttgccggt ttccagcgtg gtaaacaatg gcgcgacgct tacgccatct
     2101 ccgacctgtt tgtcatgcca tcggtttccg aacctttcgg cttgacgcct tacgaagcca
     2161 tcgcttacgg ttcaccgtca ctcataagcc ggcaatcagg agtcgcggaa gtgttcagca
     2221 actgcctgaa agttgacttc tgggacgaga atgaaatggc caacaaaatt gtcgcggtac
     2281 tgcgcagcgg gcccctgcgt gacgagctgc gctccaacgc ccaggaagaa tacgtcaagc
     2341 tcagctggag cgacgccgcg gaacgcatca tggaccacta ccggcaattt acccagcggg
     2401 tggccgtatg aaatcgattg tcttttacct gcacgcccac cagccctggc ggctgcgtca
     2461 ttacacgatc tttgatatct cccaaggcca caattatttc gacaccgacc cggcccacga
     2521 taacgacaac cagcgtgtcc tgcagaaagt ggcccaaaag tcctacggac cgaccaatgc
     2581 caaacttaag atgttgctgg ataaataccc cgattttaag ctcaacattt ccatcaccgg
     2641 cactttgctg gaccagctgg agcgctgggc gccggatgta ctggccagtt tccaggagct
     2701 ggcggccacc ggccgggtgg aaatcgccac cgaaacttac tatcacacat tggctttttt
     2761 ctatgaccgc gaggagtttg atcgccaagt caagcagcac aaagacaaga ttgaacagct
     2821 tttcggccaa acgcccgtgg tttttcgcaa taccgagcta gcttataaca atgagctggc
     2881 ccagtgggcc gaagccgccg gtttcaaagg catcttagcc gagggctggg acccggtctt
     2941 gggctggcgc agcccgaatt tcatgtaccg tccagccggc acccacagca tcaaactatt
     3001 gcttaaaaac taccggatca gcgacgacat cgctttccgt ttcggcgaca gcaactggca
     3061 ggaatggccc ttgaccagcg aaaaattcag ccactggctg gaccagaccc accctgccga
     3121 gatgttcaac ttattcatgg attacgagac tttcggcgaa caccaatggg aggacaaagg
     3181 catttttgat tttctggagt acttgccgga aaaatggctg tcgcaagacg gcaacacttt
     3241 catgaccttc accgaggcta ttgacaaata cgaacccaag gacgaagttg atatgcccta
     3301 caccgtgacc tgggccgaca ccgagcgtga tctgacagct tgggtcggca accgtatgca
     3361 gcagcaggca atcaatgccg tttacagcct cgctgataag gtagcgcatt gcggtgacca
     3421 aaaactgatt gacgattggc gccggctgca gaccagcgac cacttttact atatgtgcac
     3481 caagtggttc aatgacggcg atgttcacgc ctatttcagc ccctatgaat cgccctatga
     3541 ggc
//
LOCUS       DASPTF010000005         3688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_582039, whole genome shotgun sequence.
ACCESSION   DASPTF010000005 DASPTF010000000
VERSION     DASPTF010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582039"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="VEH48_00125"
     CDS             complement(<1..1328)
                     /locus_tag="VEH48_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXY17818.1"
                     /translation="MDRTAPGRSRGNPPSSRRRINWWYGLLLAVCVLFIGRLFYLQVV
                     RHDYYHKAALSDQLKQYTIAPQRGIIEAQDGSGTVPLVLNQELYTLYADPTLVKDPTS
                     ESAKLGPIAGVSEGQYVNLMKTRNTRYVVLAKRLSEAQNQQITQLKLPGIGTQGQDYR
                     TYPDGDLASQLLGFVNDNGVGEYGIEQALNSELTGKAGTLKAITDVNGVPLAASKDNV
                     QISPVAGDNIVLTINLAMQQQLETILQQGLKNAESPSGSALIMDPYTGAIKAMANWPT
                     YDPSQYFNVTDPSLFQNAAVSSPLEVGSVMKTLTTSAALNLGVITPTTSYDDPGSWTL
                     DGSTIHNVEEDGGPGVHDIAQVLNLSMNTGATWMLMQMGGQTGVVTQKGRDIWHDYMV
                     NHFQLGKPTGIQQGYEASGYIPDPDSGYALQLTYANTAFGQAMTATPLQMG"
     gene            complement(1345..1689)
                     /locus_tag="VEH48_00130"
     CDS             complement(1345..1689)
                     /locus_tag="VEH48_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17819.1"
                     /translation="MTYSSSVAMSRRGYARRNQNSVAFTDTSRTLGPVSNTIILIVLA
                     CLIGLLYLTQVTKTNSYGYIINNLQQQQSQLQSQKSDLEISAARLQSLDRVAASSAAK
                     SLVSVAPAGSIQ"
     gene            complement(1837..2757)
                     /gene="rsmH"
                     /locus_tag="VEH48_00135"
     CDS             complement(1837..2757)
                     /gene="rsmH"
                     /locus_tag="VEH48_00135"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HXY17820.1"
                     /translation="MPKHHHQTSAASGPHLPVLLTAVLSVLDPQKGESYLDLTAGYGG
                     HAQALLERTSKPEAAVLVDRDDQAVAHLRKQFKSAPPEIVHSDFLGACRTLSEQGRKF
                     DLILADLGASSPHFDQASRGFGIMQSGPLDMRMDQSQALTAEAIVNKWKEADIAKVLR
                     DFGEEPRAHSIARRIVAARPLRTTDELAAVVGRGWRRPSRLHPATRTFQALRIAVNSE
                     LSQLEQSLPLIERLLRPGGRMAVISFHSLEDRIVKRFLAEQAGGNYDSPLKLLTKKPI
                     TAGRDEIVSNPRARSAKLRAAAKIKTNRKE"
     gene            complement(3086..3442)
                     /locus_tag="VEH48_00140"
     CDS             complement(3086..3442)
                     /locus_tag="VEH48_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17821.1"
                     /translation="MGDYFERKLDEKRRLSIPAEFRDEFADGVVLTIGFKNYLHLYPK
                     AVWDQQVEPQLKGNILDEATADLNVRFRMGKAESALDAKQGRIQIPQHLLKHAGIGRE
                     IVAVRAGSYWRLSRKE"
BASE COUNT          688 a          987 c         1069 g          944 t
ORIGIN      
        1 cccatctgca gcggcgtggc agtcatggct tggccgaaag cggtattggc gtaagtcagc
       61 tgcagggcgt agccgctgtc tgggtcaggg atatagccgc tggcctcgta gccctgctgt
      121 atgccggtcg gcttgccaag ctggaaatgg ttgaccatat aatcgtgcca tatgtcacgg
      181 cccttttggg taaccacacc tgtctggccg cccatctgca tcagcatcca ggtagcgccg
      241 gtgttcatgg acagattcag gacttgggcg atgtcatgga cgcccggacc gccgtcttcc
      301 tccacgttat ggatcgtact gccgtccagc gtccaggatc ccgggtcgtc atagctggtg
      361 gtcggcgtta tcactccaag attcaacgcc gcgctggtgg tcagagtttt catgaccgaa
      421 ccgacttcca aaggtgagct gacggcggcg ttttggaaaa gactgggatc ggtgacattg
      481 aagtactgcg atggatcgta agtcggccag ttggccatgg ctttgatagc gccggtgtat
      541 ggatccataa taagcgcgct gccggacggg ctttcggcgt ttttcaaacc ctgctgcagg
      601 atagtttcca gctgctgctg catcgccagg ttgatggtca ggacaatgtt atcgccagcc
      661 accgggctga tttggacatt atctttactg gccgccagcg gcacgccgtt aacgtcagtt
      721 atggctttca gagtgccggc cttgccggtc agttcgctgt tcagcgcttg ctctatgccg
      781 tactcgccta ccccgttatc attgacgaag cccagcagct gcgaagccag atcgccgtcc
      841 ggataagtcc ggtagtcctg cccctgggtg ccgatgccgg gcaatttcag ctgggtaatc
      901 tgctggttct gtgcttcgct caagcgttta gccagcacca catagcgcgt gttcctggtt
      961 ttcatcagat tgacgtactg cccttcgctt acgccggcaa tcggccccag cttagccgat
     1021 tcgctggtcg gatctttgac cagagtcggg tcggcgtaca aagtataaag ttcctgattg
     1081 agcaccagcg gcacggtgcc gctgccgtct tgggcttcaa tgatgcctct ctgcggtgcg
     1141 atggtgtact gcttgagctg gtcactgagc gcggccttgt gataataatc gtgtcgtacg
     1201 acctgcagat aaaacagccg gccgataaaa aggacgcaga cagccaaaag cagcccgtac
     1261 caccaattga tccggcggcg ggagctgggc ggattgccgc ggctgcggcc cggagcggtt
     1321 ctatccatcg gcttagccgg tttactactg gatcgaccct gctggcgcga ccgaaaccag
     1381 gcttttagct gccgagctgg cggccactct gtccagcgat tgcaggcggg cggcgctgat
     1441 ttccaaatcg gacttttggc tctgcagctg tgactgctgc tgctgcaaat tattgatgat
     1501 gtagccgtag ctgttggtct tagtcacctg ggtcaaataa agcaggccta tcaaacaggc
     1561 caggacaatt aggatgatag tgttgcttac cggtcccagg gtgcgggagg tatcggtgaa
     1621 ggctactgag ttttggttgc gccgggcgta gccgcggcgg ctcatggcaa cggagctgga
     1681 gtatgtcata tttttgtttt ttggacttgg gcggcgtccg ccggaggcgg acgccgggat
     1741 cccggcaaag ccgcttaagt gccctttaag accctaacgt ggactttgta ggcccgggaa
     1801 gtactcttta cctgaatagg catgagcttt gtccttttat tcctttctat ttgtttttat
     1861 tttcgctgcg gcgcgcagct tggcgctgcg ggctcgcgga ttggaaacga tttcatcacg
     1921 gccggcagtt atcggttttt tggtcagaag tttcaatgga ctgtcgtagt tgccgccggc
     1981 ctgctcggcc aggaagcgct tgacgatgcg gtcttccaaa ctatggaagc tgattaccgc
     2041 catccggccg cccggtctca acagccgctc tataagcggc aggctctgct ccagctggct
     2101 gagttcggag ttgacggcga tgcgcagggc ctggaaggtc ctggtggccg gatgcaaccg
     2161 gctgggccgg cgccatccgc ggccaacgac ggccgccagt tcatcggtgg tgcgcagcgg
     2221 ccgcgccgcc acgattcgtc tggcaatgct atgcgccctg ggctcttcgc caaaatccct
     2281 caggaccttg gcgatgtcgg cctctttcca cttatttaca atggcctcgg cggtcaaagc
     2341 ttgcgactgg tccatgcgca tgtccagcgg cccgctctgc atgatgccga aaccacggct
     2401 agcttggtca aagtgcggtg aacttgcgcc caaatctgcc aaaatcaggt caaatttccg
     2461 gccctgctcg cttaaggtcc ggcaagcacc taaaaagtcg ctatgtacta tttccggtgg
     2521 cgcagactta aattgcttac gcaaatgcgc taccgcctgg tcatcgcggt ccaccaggac
     2581 ggcggcttca ggcttagacg tgcgctccag cagcgcctgg gcgtggccgc cgtaaccggc
     2641 ggtcagatcc aggtagctct cccctttttg gggatccagc acgcttaaaa ctgctgtgag
     2701 taatactgga aggtgcggtc cgctcgcggc ggacgtttgg tggtgatgtt ttggcatttt
     2761 tgtttttgtt tttgcaaggt cacctaatta gcagtcagct ttttgttttg gttggttttt
     2821 tgtttttgtt taccccgtca gatgacaggg agttgaggcg aagaagcgcg aacaaaaagt
     2881 tcttaccttt tcgcttcgaa agttcttgtg agagactttt gtgaggtgga gttttgggag
     2941 gtgttacgat tgagaaatgt gcgagggttt tgataacggt ggttaccctt aatttccatc
     3001 ggctgactgc caaatagctg acctcgaacc gctattggct cgtctttttg cgaacgccgg
     3061 attgtcaagg tgcggcttgc cgcctctact ctttgcgcga cagacgccaa tagctgccgg
     3121 cgcgcacggc cacgatttcg cggccgattc cggcgtgctt cagcaggtgc tgcggaattt
     3181 gtatccgacc ctgtttggcg tctagcgcgc tttccgcttt ccccatccgg aagcggacat
     3241 tcaggtcggc tgtggcttca tctaagatgt tgcctttcag ctgcggttct acctgttggt
     3301 cccaaacggc tttggggtag aggtgcaagt agtttttgaa accgatggtc agaactacgc
     3361 cgtccgcgaa ctcgtcgcga aattcggccg gtattgataa ccgccgtttc tcgtccagct
     3421 tgcgttcgaa gtagtctccc atggcttctc ttttttaaat agtggttttt gttttgaagg
     3481 ggcgcctgtt ttgggctacc cacttttccc cactaacatc aatatataac ccactcctac
     3541 ccacacgcaa gtgttttata aaaaaagaga ccgctacttg cggactcttt tccacagatt
     3601 ggcgataact atagcttacg cacaatatat aggggagtaa gctatcccgc agacgctagg
     3661 gttgttcaat gtgagaaggt gtgtttgg
//
LOCUS       DASPTF010000006        11806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_583462, whole genome shotgun sequence.
ACCESSION   DASPTF010000006 DASPTF010000000
VERSION     DASPTF010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 11806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11806)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11806
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583462"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..3236
                     /locus_tag="VEH48_00145"
     CDS             117..3236
                     /locus_tag="VEH48_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HXY17822.1"
                     /translation="MQSTSGSTSTSTTLQFASCSGGGSSLAGDYNSSGTTGNTITYTT
                     AGAGIVIQDASTPLSGNLLTIQNNAATFNYLTLNLSSSVPHLKVYGASSTNYADIYYD
                     AGSSTAYFTASSGTTSVGSGSGPVTINAGSGAAVTITGHATSTWSLDAGNLTIDVTSG
                     STPTLNLGTGAQAKNIFVGSATSGVAVDVEGATMDVGDSTLAKTIHIGGVDFAATETI
                     AIGSNASSVETITVGTTNSTSSLALNSGSNNITLISNGASAGTVVKSNTNSSSAFQVD
                     NSSGIGLFRVNTSSNNSNNLLTNPSIETGGSPPSGWAAKNGSGTSTPTQVSSPAYDGS
                     YSMQVVTSGTATNAGVTQSLSLSTSTAYVLAVYVNSLSDFVSTTGAGTFEMGYGTGGS
                     DNLCLTAQKVPAASWTRLTCSFTTAASLTSPYIYFKQTDSTPRTFFIDSAVLETDPNA
                     TSKWQAGQITLNGVINSPVIISPQQDSTNTFLVQNSEGINVFGVDTTDTNLVANPGLE
                     VNTTGWGYNGTPSGSAGIFRDTSTPYLGVASLKLITTSTATDGAKFTFSPNTLLQAST
                     TYAMSFYAKLSSGSLATLTFGRSENGSQTSCVTTGSINTTWQRYSCSFTVGGTIGATP
                     YIYISPGTGTQTMWIDAVSLEPGSTVTPYGSSNIYLNGVITSPVNFQNKADSTTAFQI
                     ENAGSAVLFTADTADSRVVIGSNIQSNTTITLFQLNSDSNFNSDETTVGCSTSGSGTN
                     LGAMYYNSTTEAIRACINGSWEDIVSTAALGLQLFGVVPDSGATPGDLLGITSQGSGP
                     CKVYMGSATTAIRWTGCIAYSGGRKIVVPAQSSNQSVTLTSGHWEHVCLSGTAGAVTI
                     TDSTSETANLGTVSFPSATAPILCLADITVNGTAITAIYDVRTFTTTTKEFVNVITTA
                     APLGTIVQQTTTPGSVTKAAATANLASLKGVIVASAGTTTANTINAIIATNGPAYVKS
                     SAGTVQAFVTEGATTAGYAITTTTQTSTIKDQNQYSVLGVAESTRSTTCSTTADDCRT
                     SLLVNLSIQ"
     gene            3266..4966
                     /locus_tag="VEH48_00150"
     CDS             3266..4966
                     /locus_tag="VEH48_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXY17823.1"
                     /translation="MGAVVRKMVRSPYFWLAVIGLLLFSITFFSGFVDDDLAQVVNQN
                     HVHNLHGLIWSFRIGEGLFRPLMFSYFTLCYALFGLHAWGYHLFEVAFGLANVLLVYW
                     VLRQFFSQNLAFWLAVVFLVSPLNVDAVSYVSHIQDVLFLFFGLLCLRVVKSGVRNWL
                     KTLLVIVLLLASLLSKETGALFLPMIIIFTWWFTQRGQKAIYAGAGLAIVIYVFLREI
                     APNDHSILPPFAPVSHFGLGMRLLQIPSIIWFYLSNFIFPRHLSISRLNVATDVSLVH
                     FWLPLIAELIILGFIIWLGFFIYRGHKKHWRTFCFFFIWLALGLGFHSQIKPIFDATY
                     SNWWFYFPMVGLLGLAGVAITAFWPRGYDWKHQPWLVGFLVIWLLFLLSQNVYRQLNF
                     HSEKNLVTHDISVNSNSYLLQNEYGSLLISEGRYAEAQIPLYKSISLLTDRNSAWDDL
                     GVAYYSQGNAIEARDYFKKAVEYYPDDVEGYYNYASSLINSFQPDGIKDFLSMAIKRF
                     PDDVFLWQLRAAAYDLYGDSVESARAQTKAYQLAREQHVQLSVEHRPGEPFNIKLQTQ
                     "
     gene            4963..5499
                     /locus_tag="VEH48_00155"
     CDS             4963..5499
                     /locus_tag="VEH48_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17824.1"
                     /translation="MTEEQIQSSKTVNRRSLPKWSLLILVIFLATSGFLAWRFWPRPL
                     ISPAEKQKISFLIFVPSSPYQVVRKTVAYDSQKKILTYQAKDSTGSSVFVVTEQTEPD
                     LLSLGGQDFYNKFVGALGDYDDIDTAAGPVSLTRPSKLGSQTAVLVAQNTLVFVRPNH
                     NLDENDWQGFFNTMQTYH"
     gene            5561..6217
                     /locus_tag="VEH48_00160"
     CDS             5561..6217
                     /locus_tag="VEH48_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HXY17825.1"
                     /translation="MSSPESNPDAQNQTRVDLLVVLGKNIGVESGPPEILRDNFSLSH
                     ESRFDALAAGELWHPGMEIIFSSGSTVPGLPSEAAAMKKYMQTHVNNRFGRPKVPEDA
                     ILLEEKSIDTAGNAEEVARLLKERTYRCIGLVSVGYHVNNAARLFKNYGVPINQKFAS
                     EEIVRQISPRHERIVENWKKTKRIKDEHRKEMLRTILLLTRIDRKGKLLRKITSRSRG
                     "
     gene            complement(6214..6972)
                     /locus_tag="VEH48_00165"
     CDS             complement(6214..6972)
                     /locus_tag="VEH48_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXY17826.1"
                     /translation="MQKVSIVIPVYNEIATLAKVVKRVEAANVGGLKKEIILVNDGST
                     DGTEERLKLYRNRHRVINNSRNRGKGYALRCGFANASGDIIITQDADMEYDPNNYSRL
                     LKPILSGECDVVYGSRFKLGRRYRLSVPASALNLWANKLLTKLTNLILGTGLTDMETG
                     YKVFKSEIVQKVDLRQDGFGFEPEFTSRIAQLGYPIQEVPISYDPRSRADGKKIKWRH
                     GLQAVWVLLNAGVDGSRLLERSAPIPDKKVSTSL"
     gene            7024..7464
                     /locus_tag="VEH48_00170"
     CDS             7024..7464
                     /locus_tag="VEH48_00170"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HXY17827.1"
                     /translation="MKWEAYWRRRSLKHDFSRFVVVGCLGFGINLLILSICYKMLGWP
                     IFWSQLLGSELALFSNFILHHNWTYQGHNSQKSIKRLLIEFHTTSWMAIIGTTVLVSV
                     GVDVLKLHYILALVIASLIALGWNFGWTRFYIWQRHQPNVLKDS"
     gene            7489..11802
                     /locus_tag="VEH48_00175"
     CDS             7489..11802
                     /locus_tag="VEH48_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17828.1"
                     /translation="MARGKSLVLSVKYLLMAAFKFTRLGGLGKKRLAALGLVILIAGV
                     AGSQLYRAHHRYDLSPAELSLLSKPSIQLSKVKLSKTAVSYNAQPDADIKTVSVNITN
                     PENSTGQFPYTATINNKASAGMSFGDSQGDFSFNIIPLDSLSSGKYGQGQTIFAGSHN
                     SRQVLTFMRNGVKEDLLLPKAPGNSFSFHWQLQLGSELQARLMPNGSVGIFSPDPNLY
                     GNLQIEDAKSQALINTARQNGPRDNLVFILPQPYIKQANGRIDTADVSFSLAGNILTL
                     NAYKLKAADYPLTIDPSVVVTTTADFQTDNLDDGMIDYSISGQINRGAISLGKIGTWA
                     YTHNSTNDGATFSAGFIDQRNGDTVVAYNGYLYVIGGIIGSSIDDCTASGYCNGVQFA
                     PINSNGTVGAWHYTHNSTDDGTTFVSGFNTARYIFTAVAYNGYLYVIGGRSNTTANDC
                     TSGGTGCSGVQFAPINSNGTVGAWHYTHNSTDDGTTFVSGFIQARNIHSSVVYNGYLY
                     IIGGHTSGGSNDCSSGSTCSGVEYAPINSNGTVGAWHYTHNSTDDGTSYVSGFSQTRY
                     GMAAVAYNGYLYVSGGISGTSTNDCTATNNYCNGVLFAPINSNGTIGAWNYTYNGNSC
                     STWTSPCNNGFTQARSESASVAYNGYLYVIGGQSGTTGNDCPATINYCSGVQFAPINS
                     DGTVGAWHYTHNSTDDGTTFVSGFTQVRNLPGTTAYNGYLYVIGGYSGSSTGSDCTSN
                     LGCNGVQFTSINTGTPSYTGLATAGTWAYTHNSTNDGGTFSAGFTQARSDQTSVAYNG
                     YLYVIGGYSGSTSGNDCTDGSSAFDCNGVQFAPINSNGTIGSWNYTYNSNSCSTYISP
                     CNGGFTQARADQTSVAYNGYLYVIGGRSRGTGADCTTLKSGLYYCNGVQFAPINSDGT
                     IGAWHYTHNSTDDGATFSAGFTQTRAGQTSVAYNGYLYVIGGRSGTSANDCTASAANY
                     CNGVQFAPINSNGTIGSWNYTHNSGNDGTSFVAGFTQGREGQTSVAYNGYLYVIGGQS
                     LTSTNDCTATSDYCNGVQFAPINSDGTIGAWHYTHNSTDDGTSFVSGFTQARYYHTTV
                     AYNGYLYVTGGASGSSANDCTATTNYCSGVQFAPINSNGTIGSWNYTHNSTNDGGTFS
                     AGFTQARYSQTSVAYNGYLYVIGGFSNAGANDCVSGVCNGVQFAPLNSPAQRAHYERV
                     VDTGSSGNTVNSVVYNGTAVCGAQLSYALAGSSNAFGSFTTIYNAIPGITYTINQTNE
                     RYVLLDITLDDTQCGTASNITDITVNYSASNSPPAAPTLSAPSSGVTGVSVTPLFQMS
                     TTDPDGDYVEYDIKLYQSDCSTLVRDVDETSSQTGWSGQNANSGNAYTSGTTASHTYQ
                     APGLSLSTTYCWAARAIDPGGSNTWSSYSATQTFTTTNGTSAPVNINGGVNINGGTTI
                     Q"
BASE COUNT         2853 a         3140 c         2872 g         2941 t
ORIGIN      
        1 ttatatttaa taatacggcg gggtctaata ctattacctt aaccgctcaa ggcagtaacc
       61 ccaccagctc ggtaacattg tatctaccgc ttgcgggtgc caccggcgac cagtgcttgc
      121 agtcaactag tggttctacc agcaccagca ctaccttgca gtttgcttcg tgcagcggcg
      181 gcggctcgtc tttagctggc gactacaact ccagcgggac tacaggcaac actataactt
      241 atacgacggc cggtgccggc atagtcattc aggacgcctc tacgccgctg tctggtaatt
      301 tattaactat tcaaaacaac gccgccactt tcaattactt gacacttaat ctgtccagta
      361 gcgtgccaca cctgaaagtt tacggtgcca gcagcaccaa ttacgccgat atttattacg
      421 atgcgggctc ttccacggcc tattttacag cctctagcgg caccaccagc gttggcagcg
      481 gcagcgggcc cgtaactata aatgccggct ccggcgcggc ggttaccatt accgggcacg
      541 ccacttccac ctggtcgtta gacgccggca acttaacgat tgatgtgacc agcggctcaa
      601 ctccaacgct taaccttggc accggcgccc aggctaaaaa tatttttgtc ggctcggcca
      661 ccagcggtgt tgccgttgat gttgaaggcg caacgatgga tgtcggcgac agtacactgg
      721 ccaagacgat tcacatcggc ggggtagatt ttgccgccac cgaaactata gctataggct
      781 ctaatgccag cagtgtagaa accatcactg ttggtaccac caacagcacc agcagcctgg
      841 cactgaactc cggcagtaat aacataaccc ttatttcaaa tggagccagc gccggcacgg
      901 tagttaaaag caacaccaac tcttcatcgg cctttcaagt agacaattcg tcgggaatcg
      961 gactattccg tgttaacact agctccaaca actcaaataa tttgctgact aacccaagta
     1021 tagaaaccgg cggtagtccg ccgagcggat gggcagccaa aaacggctcg ggtacttcta
     1081 cgcctaccca ggtttcatca ccagcttacg acggttcgta ctctatgcag gtagtgactt
     1141 caggtaccgc caccaacgcc ggcgtgacac aaagtctttc tttatcaact tccacggcct
     1201 acgtactggc ggtctatgtc aactcattaa gcgactttgt aagcacaacg ggggcgggca
     1261 cctttgaaat gggctatggc actggtggtt cagataatct ctgcctgacc gcgcaaaaag
     1321 ttccagccgc tagctggacg cgcctgacct gttcgttcac gaccgcggcc tcgttaactt
     1381 cgccctatat ttactttaag cagaccgata gtacaccccg tactttcttt attgattcag
     1441 cggtcttaga aaccgatccg aacgccacca gcaagtggca agctggtcag attaccctaa
     1501 acggggtaat caacagtccg gtaattattt caccccagca agatagtaca aatactttcc
     1561 tggtgcaaaa ttccgaaggc atcaatgtct ttggagtaga tacgaccgac actaatctgg
     1621 tcgcgaaccc gggcctggaa gtcaatacta cgggctgggg ctataatggc acaccgtccg
     1681 gcagcgccgg catattccgc gacacttcta cgccttacct tggtgttgcc agcctgaaac
     1741 ttattactac atcaacagcc accgatggcg ccaagttcac gttctcgcct aacacgctgc
     1801 tgcaagccag caccacgtac gctatgagct tttatgccaa gcttagtagc ggcagcctgg
     1861 ctacgctgac ttttggccgt tcagagaacg gctcgcaaac ctcctgcgta acgaccggct
     1921 ccattaatac gacttggcag aggtacagct gctcattcac ggttggcggc actataggcg
     1981 ctacaccata catatatatt tcgccgggca ccggcactca gacaatgtgg atagacgcgg
     2041 tgtccttgga gccgggctct acggtcacgc catacggctc atccaatatc tatcttaatg
     2101 gcgtcataac ttcacctgtc aacttccaga acaaggccga ctcaaccacg gcttttcaga
     2161 tagagaacgc tggttctgcc gttttattca ctgcagatac agccgacagc cgagtggtga
     2221 ttggctcaaa catccaatca aacacgacta ttaccctgtt ccagctcaac tccgatagta
     2281 actttaacag cgacgagaca actgtgggtt gttcgacatc gggcagcggc actaacttag
     2341 gggcgatgta ttacaactca acaactgaag ctatccgcgc ttgcattaat ggcagctggg
     2401 aagacattgt ctcaactgcg gctctggggc tacagttgtt tggcgttgta ccagattctg
     2461 gcgccacccc gggcgactta ctgggtataa ccagccaggg ctcgggcccc tgcaaggttt
     2521 acatgggttc tgcaacgacc gcgatacgat ggaccggctg tatagcatac tccggagggc
     2581 gtaagattgt cgtgccggcc caaagcagca accaaagcgt aactttgact agcggtcact
     2641 gggagcatgt ttgcttgagc ggcaccgccg gggccgtaac cataaccgac agcactagcg
     2701 aaacggccaa cctgggcaca gtttcgttcc cctcagcaac tgcaccgata ctctgcctgg
     2761 ctgacataac cgtcaacgga acggctatca ctgccatcta tgacgtacgc acttttacca
     2821 ccacaactaa agagtttgta aatgtaatca caaccgctgc gccgctggga acaatagtcc
     2881 agcagacaac cactcccggt tcagtcacca aagccgcggc aaccgcaaac ctggcctctc
     2941 ttaagggtgt aatcgtggct tccgccggaa caacaactgc caacactatt aacgccatta
     3001 tcgccaccaa cggcccggct tatgtcaaat cgtcggctgg tacggtccaa gcctttgtca
     3061 cagagggagc cacgacagcc ggctatgcca taaccaccac cactcagacg agtacgataa
     3121 aagaccaaaa ccaatactcg gtacttggcg tcgcggaaag cacgcgtagc actacctgct
     3181 caacaacagc cgatgactgt cgcacctccc tgctggtcaa tctctcaatc cagtaggctt
     3241 taggtttttt gctatagtgg ggacaatggg cgcagttgtt aggaagatgg tgcgttcgcc
     3301 ttatttttgg ctggctgtaa tcggccttct tttgttcagc attaccttct tcagcggctt
     3361 cgttgacgac gacctggcgc aagtcgttaa tcagaatcat gtccacaatc tacacggttt
     3421 aatctggagt tttcgaatcg gtgaggggct cttccggcca cttatgttca gctacttcac
     3481 cctgtgctat gccctattcg gcctgcatgc ctggggttat catttgtttg aggttgcttt
     3541 cggtctagcc aacgtactcc tggtttactg ggttctgcgc cagtttttta gccagaatct
     3601 ggccttttgg ctggcggtcg tcttcctggt tagtccgttg aatgttgacg ccgtatctta
     3661 cgtatcgcac atccaggacg ttcttttctt attcttcggc ctgttgtgtc taagagttgt
     3721 gaagagtggt gttaggaact ggctcaaaac attgctggtt atagtgctac tgctggcttc
     3781 gctgctttcc aaagaaaccg gcgcgttatt tttgccgatg ataattattt ttacctggtg
     3841 gttcacccag cgcgggcaaa aagcaatcta cgctggcgcg ggtttggcaa ttgtaattta
     3901 tgttttctta agagagatcg ctccgaatga tcattctatt ctgccacctt tcgcgccagt
     3961 atcccatttt ggtcttggga tgcgcttgct gcagattccg tcaatcatct ggttttacct
     4021 gagcaatttc atttttccgc gccatttatc aatatcgcgc cttaatgtgg caacagacgt
     4081 gagcttggtt catttctggc tacccttaat cgctgaattg atcatcttgg gattcattat
     4141 ctggcttggt ttctttatat accgcggcca caaaaaacac tggcggactt tttgtttctt
     4201 ttttatctgg ctggcactgg gtcttggctt ccactcacag atcaaaccaa tctttgacgc
     4261 cacctattcc aactggtggt tttatttccc gatggttggt ttgctgggat tagccggagt
     4321 ggcaataaca gccttctggc ctcgaggtta tgattggaag caccagccat ggctagttgg
     4381 cttcctggtg atttggcttt tatttctgct aagccaaaac gtttatcgtc agcttaattt
     4441 ccacagcgaa aagaacttgg tgactcatga catctcagtt aattcaaata gctatctttt
     4501 gcagaacgaa tacggctcct tgctgataag cgagggcagg tatgccgagg cccagatacc
     4561 gctatataag tcaatctctc ttttgaccga ccgcaattcg gcctgggatg atcttggagt
     4621 ggcctactat agtcagggta acgccatcga agcccgggat tatttcaaga aagcggtgga
     4681 atattatcct gatgatgtag aaggatacta caattatgcc agcagtttga tcaactcctt
     4741 ccagccggat ggaattaagg atttcttgag tatggctatc aagcggttcc ccgatgatgt
     4801 tttcttgtgg cagctgcgcg ccgcggctta cgacctgtac ggcgattcag tagaatcggc
     4861 gcgggcgcag accaaggctt accagttggc ccgcgagcag catgtacaat tgagtgtgga
     4921 acatcggccc ggagaaccgt tcaatatcaa actgcaaacg caatgacaga agaacagata
     4981 caatcctcaa agacggtaaa cagacgttct ctgcccaagt ggtctctctt gatcctcgta
     5041 atcttcttgg cgaccagcgg tttcctggca tggcgcttct ggccccgacc attaatcagt
     5101 ccggcagaaa agcaaaaaat ttcctttctt atctttgtgc ctagctcgcc atatcaggtg
     5161 gtacgcaaga ccgttgccta tgattcacaa aaaaagattc tgacttacca ggccaaggac
     5221 agcacaggaa gttcggtttt tgttgttaca gagcagacgg agccggacct gttgagcctt
     5281 ggcgggcagg atttttacaa taaatttgtc ggggccttgg gtgattatga cgatattgac
     5341 accgcggccg gcccggtcag cttaacccgg cctagcaaat tgggcagtca aacggctgta
     5401 ctggttgccc aaaacacttt ggtgtttgtc cggcctaacc ataatcttga tgaaaacgac
     5461 tggcagggat ttttcaacac catgcagact tatcactaaa tcaagcctcc caagccggtt
     5521 tttgaccccc gccctttatg tgctatggta atacttaatt atgtcgtcgc ctgagagcaa
     5581 tccggacgcg caaaaccaaa ctagggtaga tttattggtc gttcttggca agaacatagg
     5641 tgttgaatca ggcccacccg aaattcttag agacaacttt agcttgagcc acgagagccg
     5701 ttttgacgct ctggcggcgg gtgaattatg gcacccgggg atggagatca ttttttcctc
     5761 gggttctaca gtacccggtc tgccatccga agcagctgcc atgaaaaaat atatgcagac
     5821 gcatgtaaat aatcggttcg gcaggcccaa agttccagaa gacgccatat tgctggaaga
     5881 aaagtctatt gacactgccg gcaacgccga agaggtagcg cgtttattaa aggaaagaac
     5941 atatcgatgc atcggtttag tatccgtggg gtaccatgtg aacaacgctg ccagactgtt
     6001 caaaaattac ggagttccga ttaaccaaaa attcgcttca gaggaaatcg ttcggcagat
     6061 ttcgcccaga cacgaaagaa tcgttgaaaa ttggaaaaaa acaaaacgga ttaaagacga
     6121 gcaccgcaag gagatgctcc ggacaatctt actactaacc cgaatcgacc ggaaagggaa
     6181 gctacttaga aaaatcacca gccggtcgcg cggctaaaga gaggtggaaa ctttcttatc
     6241 gggtattggc gccgatcttt ctaatagccg cgatccatct actccggcat tcaacaaaac
     6301 ccagactgcc tgcaagccat ggcgccattt gattttcttg ccgtcggcac ggctgcgcgg
     6361 gtcatatgaa atcggcactt cttggattgg ataaccaagc tgggctatcc gtgacgtaaa
     6421 ttccggctca aagccaaaac cgtcttgccg gaggtccact ttttgaacta tttcgctttt
     6481 aaaaactttg tagccggtct ccatgtccgt taggccagtg cccaaaatta aattggtcag
     6541 cttggtcagt aatttgttgg cccataagtt caaggctgat gcgggcacgg atagcctgta
     6601 tctacgccca agtttgaagc ggctgccgta gaccacatcg cattcaccgc ttaggatcgg
     6661 ctttaagagc ctggagtaat tgttggggtc gtattccatg tcggcgtcct gggtgataat
     6721 gatatcgccg ctggcattgg cgaagccgca gcgcagagcg taacctttgc cccggttgcg
     6781 tgaattattt ataacccggt gccggtttct atatagctta agccgttcct ctgtcccatc
     6841 ggtgctgccg tcgttgacca gaatgatctc ttttttgagc ccgccgacat tggcggcttc
     6901 aactctctta actaccttcg ctaaggtggc aatttcgtta tacaccggaa ttacgatgga
     6961 gactttttgc atttttgtct ggtttttata tcatcccttt acacttatgc taccataacc
     7021 gttatgaaat gggaagccta ttggcggcga aggtccttaa agcacgattt tagccggttt
     7081 gtggtagtag gctgcctggg ttttggcata aacctgttga ttttgtcaat ctgttataaa
     7141 atgctgggct ggccgatttt ttggtctcag cttcttggca gcgagctggc gcttttcagt
     7201 aactttattc ttcaccataa ctggacgtat cagggtcata attcccaaaa atcaatcaag
     7261 cgtttgctta tagaatttca cactacatct tggatggcta taatcggcac caccgtactc
     7321 gtgtcggttg gcgttgacgt acttaagctg cactacattc tggcgctggt gattgcttcg
     7381 ttaatcgctc tgggctggaa tttcggctgg acgcgctttt acatttggca acgccaccaa
     7441 ccaaatgtat taaaagattc ctagtgctat tttgctataa taacacttat ggctaggggg
     7501 aagtcgctag tgctaagcgt gaagtattta ctgatggctg cttttaagtt tacgcggctt
     7561 ggcgggttgg gcaaaaagcg gctggcggca cttgggctgg taatattaat cgccggtgtc
     7621 gccggcagcc agctttaccg ggcgcatcac cgctacgacc taagcccggc agagctaagt
     7681 ttacttagca aaccctcaat ccagctaagt aaagtaaaac taagcaaaac cgcggtcagc
     7741 tataatgccc agccggacgc cgacattaag accgtcagcg ttaacataac taacccggaa
     7801 aatagcacag gccagttccc ctacaccgcc accattaata ataaagcttc agccggtatg
     7861 agtttcggcg acagccaagg cgattttagc tttaacatca tacctttgga tagcttgtcc
     7921 agcggaaaat acggtcaagg gcagacgatt tttgccggct cccataattc ccgccaggtg
     7981 ctgactttta tgcgcaatgg cgtcaaagaa gatttactgc tgcccaaagc tcccggaaac
     8041 agttttagtt tccactggca gctgcagctg ggcagcgaac ttcaggcgcg gctgatgccc
     8101 aacggcagtg ttggtatctt ctcgcccgac cctaaccttt acggtaattt acagatagag
     8161 gacgctaaaa gccaggcttt aataaatacc gcccggcaga acggccctag ggacaatttg
     8221 gtattcatcc tgcctcaacc ttatatcaag caggccaacg gccggattga taccgcagat
     8281 gtttcattca gcctagccgg taacattctg actctaaatg cctataaact aaaggctgct
     8341 gattatccac tgactattga cccttcggta gtggtaacaa caactgcgga ctttcaaacc
     8401 gataaccttg acgacggcat gatagattat tccatttctg gtcaaatcaa tcggggggcc
     8461 atatctttgg gcaagatagg cacttgggct tatacccaca attccactaa cgatggtgcc
     8521 acattttcgg ccggatttat tgatcagcgc aatggggata ctgtagtagc ttataacggc
     8581 tacttgtacg tgataggcgg gattattggc agcagtatcg atgactgtac ggctagtggt
     8641 tactgtaacg gcgtgcagtt tgcgccgata aattctaacg gcaccgtcgg cgcctggcac
     8701 tacacccata acagtactga tgacggcacg acgtttgtgt ccgggttcaa cacggctcga
     8761 tatattttta ccgcggtagc ctataacggt tatctgtatg ttattggggg gagaagcaac
     8821 acaaccgcca atgactgcac ttccggtggt acaggctgct ccggcgtgca gtttgcgccg
     8881 ataaattcta acggcaccgt cggcgcctgg cactacaccc ataacagtac tgatgacggc
     8941 acgacgtttg tgtccgggtt catacaagcg cgtaatatac attcctccgt cgtatataac
     9001 ggctacctgt acatcatagg cggccacacg tctggcggca gcaacgactg ctcctcgggt
     9061 agtacttgct ccggcgtgga gtatgcgccg ataaactcca atggcaccgt cggcgcctgg
     9121 cactataccc ataattctac tgatgatggc actagttacg tatctggatt cagccagacg
     9181 cgatatggca tggcggctgt ggcctacaat ggttacttgt acgtgagcgg tggaatctcc
     9241 ggaaccagta cgaatgactg tacggctacc aataactact gcaacggcgt gctgtttgcg
     9301 ccgataaact ccaatggcac catcggcgca tggaactata cctataatgg caattcctgc
     9361 tccacctgga cctcaccgtg caataatgga ttcacccagg cgcgaagtga atcagcctcc
     9421 gtggcatata acggatatct gtatgtgatt ggcggtcaga gcggcacaac cggcaatgac
     9481 tgtccggcta ccatcaacta ttgctccggc gtgcagttcg cgccaataaa cagcgacggc
     9541 acagtcggcg cctggcacta tacccataac agtactgatg acggcacgac gtttgtgtcc
     9601 gggtttacac aagtgcgcaa tctgcctggc acgacagcat ataacggcta cttgtatgtt
     9661 atcggggggt atagcggcag ttccactggc agtgattgta cgtctaatct tgggtgcaat
     9721 ggggtgcagt ttacttctat aaacactggg acgccgagct acaccggact ggctacggca
     9781 ggcacctggg cctacaccca caattccacc aacgacggcg gaactttttc agccggcttt
     9841 acccaggcgc gttccgacca aacttcggtg gcatataacg gctatctgta tgtgattggc
     9901 gggtatagcg gctctaccag tggcaatgac tgtacagacg gatcaagtgc atttgactgt
     9961 aatggtgtgc agttcgcgcc aatcaactcc aacggcacta ttggcagctg gaactacacc
    10021 tataacagca actcctgctc cacctacatt agcccctgca atggtggctt cacccaggcg
    10081 cgtgccgacc aaacttcggt ggcatataac ggctatctgt atgtgattgg cgggcgaagt
    10141 cgtggtaccg gtgctgattg caccacttta aaatccggtc tctattactg caacggcgtg
    10201 cagtttgcgc cgattaattc cgacggtact atcggtgcct ggcactatac ccataattcc
    10261 actgacgatg gcgcgacttt ttcagccggc ttcacccaaa cgcgtgccgg ccaaacttcg
    10321 gtggcatata acggctatct gtatgtgatt ggcgggcgaa gcggtacaag tgccaatgac
    10381 tgtacggcta gtgccgccaa ctactgcaac ggcgtgcagt ttgcgcctat aaactcaaac
    10441 ggcactatcg gcagctggaa ctatacccac aactccggta acgacggcac cagttttgtg
    10501 gctggtttca cccaaggtcg tgaaggccaa acttcggtgg catataacgg ctatctgtat
    10561 gtgattggcg ggcaaagcct tacaagtacc aatgactgta cggctacttc agactattgc
    10621 aacggcgtgc agtttgcgcc gattaattcc gacggtacta tcggtgcctg gcactatacc
    10681 cataattcca ctgacgatgg gactagtttt gtatccggtt ttactcaggc gcggtactat
    10741 cataccacgg ttgcctataa cggctatttg tatgtgaccg gtggagcgag cggcagcagt
    10801 gccaatgact gtacggctac cactaactac tgcagtggcg tgcagtttgc gccaatcaac
    10861 tccaacggca ctattggcag ctggaactac acccataact ccaccaatga cggcgggacg
    10921 ttttccgccg gctttaccca ggctcgttac agccagacct cagtggccta caacggctac
    10981 ctgtatgtga tcgggggttt tagcaatgcc ggcgccaatg actgcgtctc tggtgtctgc
    11041 aacggtgtac agtttgcgcc gcttaactcc ccggcacaaa gagcccatta cgagcgggtg
    11101 gtagacaccg gcagttctgg caacaccgta aattcagtgg tctataacgg cacggccgtt
    11161 tgtggcgccc aactcagcta cgccttggcc ggctctagca acgcttttgg ttcgttcacc
    11221 acaatctaca atgccattcc tggaataacc tacacgatta atcaaactaa cgaacgttat
    11281 gttctactag acattacgct ggatgatacc caatgcggca ctgcctctaa catcaccgac
    11341 attactgtaa actattctgc cagcaacagc ccgccggccg caccaacact cagtgcgcca
    11401 tcttctggcg tcacgggcgt atctgtcact ccgctgttcc agatgagtac aactgatccg
    11461 gatggcgact atgtagaata tgacattaaa ctttaccaga gtgactgctc gacattagtg
    11521 cgcgatgttg acgaaacttc cagccagacc ggctggagcg gccagaacgc caatagcggc
    11581 aacgcctaca cttccggtac aaccgccagc catacttacc aagcgccggg attatcgtta
    11641 agtacaactt attgctgggc ggcacgggca attgaccccg gcgggtctaa cacctggagc
    11701 agttattcgg cgacccagac ctttaccacc accaacggca cctcggcacc ggtcaatata
    11761 aacggcggcg tgaacatcaa cggcggcacc accatacagt gagccg
//
LOCUS       DASPTF010000007         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1592870, whole genome shotgun sequence.
ACCESSION   DASPTF010000007 DASPTF010000000
VERSION     DASPTF010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592870"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..2016
                     /locus_tag="VEH48_00180"
     CDS             91..2016
                     /locus_tag="VEH48_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXY17829.1"
                     /translation="MGRPVILSNGHILAGLDDFASVHDFYYPYIGLENLAHARNLSHK
                     IGLWVDGDFSWLDDGSWQINMGFEEEALVSDITAENQRLGLKLHFQDFIDHHHPAFCR
                     IIDVTNLSDKPREIRLFMHQAFQISRTGRSDTALYIPAGPYILDYKGWYSLLIYLEDQ
                     DGQPFDQYAIGNCGIEGKSGTFKDAEDGELSGNPVEHGGVDSVARLSLEIAPGQSKTA
                     RYWVVASDSQLSAEKSHKLLLNGGITERLAHTRQSWKDWLAVAAERLDHIEDKYRPLA
                     KKSLLIVKSHIDKHGGVIASCDSSIYNYGRDYYSYVWPRDGAYVLLPLIELGYKDEPK
                     RFFQFCVDTMDQSGYMLHKYQPDRAIGSTWHPQLHLSYPELPIQEDETAIVLYAISRY
                     LDMSNDEDFIRNIYPGFIKRCTDFIAEFVDEATSLPHGSYDLWEQKFATFSYTVFMTM
                     LALEASASIADRLNKPEDRDKWLAAVERIKEGMKLLVKPEGSYRKSVLLTPGGDLVFD
                     DTVDASSFQGAQLYGKADPGILENSVKAVEEKLLKDPPIGGVPRYEHDDYFLGDRSRL
                     GNPWMITTLWLAQYYLKNNRVEDAKKLMDWVVARAGVSGMLSEQVDPNTGIGVGVTPL
                     IWSHSTFLETALMLAGS"
BASE COUNT          492 a          609 c          583 g          454 t
ORIGIN      
        1 tttatcaact tcatgaatgt ctaccacgac ctaagctggc gggcttcaaa ggccaaggcg
       61 gcctgacaat gatcaaagag ggtggcgggc atgggtaggc cggtgatctt aagcaacggc
      121 cacattctgg ccggcctgga cgacttcgct tcggtccacg acttttatta cccctacatc
      181 gggttggaga acctggcgca cgcgcgcaac ctcagccata agatcggcct gtgggtagac
      241 ggcgactttt cctggctgga cgacggcagc tggcagataa atatgggctt tgaagaggag
      301 gcgctggtca gcgacatcac agccgagaac caaaggcttg ggctcaagct gcattttcag
      361 gacttcatcg accaccacca cccggcgttc tgccgcatca tagatgttac taatttgtca
      421 gacaagccgc gcgaaatcag gttgttcatg caccaggcct tccagatttc gcgcaccggc
      481 cgcagcgaca ccgcgctgta tattcccgcc gggccgtaca ttctggacta caaaggttgg
      541 tactcgctgc tgatttatct ggaagaccag gacggccagc cgttcgacca atacgccatc
      601 ggcaactgcg gcattgaggg aaaaagcggt actttcaagg acgccgagga cggcgagctg
      661 tccggcaacc cggtagaaca cggcggcgtg gacagtgtcg cgcgcttatc gctggaaatc
      721 gcgcccggcc aatctaaaac cgcccgttat tgggtggtgg cctcggactc gcagctgtcg
      781 gccgaaaaaa gtcacaagct gctgcttaac ggcggcatta cggagcggct ggcccacacc
      841 cggcagtctt ggaaagattg gctggcggta gccgcggaaa gactagacca tatcgaagac
      901 aagtaccggc cgctggcaaa aaaatcgctg ctaatcgtta aatcgcacat tgataaacac
      961 ggcggagtaa tcgccagctg tgattcgtcc atttataact acggccggga ttattacagc
     1021 tatgtctggc cgcgcgacgg cgcctacgtc ctgctgccgc ttatcgaact gggctacaaa
     1081 gacgagccga aacgattttt ccagttctgc gttgacacca tggatcaaag tggctacatg
     1141 ctgcacaaat accagcccga ccgggctatc ggcagtacct ggcatcctca attacacctc
     1201 agttaccccg agctgccaat ccaggaagac gagaccgcca tagtgctcta tgccatcagc
     1261 cgatatctgg acatgtccaa cgacgaggac tttatccgca acatctaccc tggttttatc
     1321 aagcgctgca ccgacttcat agccgagttt gtcgatgagg ccaccagtct accgcacggc
     1381 agttacgacc tctgggaaca gaagttcgca accttcagct acacggtttt tatgaccatg
     1441 ctggctctgg aagcttccgc ctccatcgct gaccgcttga acaagcccga agaccgcgac
     1501 aaatggctgg ccgccgtgga gcgcatcaaa gagggcatga aattattggt caaaccggaa
     1561 ggttcctacc gcaaaagcgt cctgctcact cccggcggtg atttggtgtt tgacgacacc
     1621 gttgacgcgt ccagtttcca gggagcccag ctttacggca aggccgaccc gggaattctg
     1681 gaaaattcgg tcaaagcggt cgaagagaag ctcttaaaag acccgccgat aggcggcgtg
     1741 cccagatacg aacacgacga ttatttcctc ggcgaccgca gccgcctggg caacccttgg
     1801 atgataacta ctttatggct ggcgcaatat tatcttaaaa acaaccgggt tgaggacgcc
     1861 aaaaagctga tggactgggt ggtagcccgg gccggcgtca gcggcatgct ttccgaacag
     1921 gttgacccca ataccggtat cggcgtgggc gtaacgcccc tgatctggag ccattccact
     1981 tttctagaaa ccgcgctgat gctggcgggc agctaacgcc gcggtttgac ttcgttgcgg
     2041 cctaagctct ttttggtttc ggtctgtttg gccatcaccc gcttgaccgg gcaatatttc
     2101 ttaagttcta ccttatccgg cgtgttgacg ctgttttt
//
LOCUS       DASPTF010000008         5817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1304807, whole genome shotgun sequence.
ACCESSION   DASPTF010000008 DASPTF010000000
VERSION     DASPTF010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304807"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VEH48_00185"
     CDS             complement(<1..1151)
                     /locus_tag="VEH48_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXY17830.1"
                     /translation="MWVGNFKQAVRSLGRAKARSFFTMLGIIIGISSVVTVVSLGEGL
                     KQQIVGQINHLGRDVITVRSGKLVNGGGNGISSLNLLAFLGASTLTARDAETISQLPS
                     VSAVAPMDFVTSSAKAGGKELDNLFIIGTSPDASEVLPETVEYGNFFNNDSSGQNFAV
                     IGTDVALQLFGEINPVGHSLMISGQNFVVSGVLARSSGSLLSVAETDFNSAILIPLTP
                     AEQLASGQTNILQILVKAKDANNLDATVSDVNSALLKNHQGQQNFTVLKQYELLHIAG
                     GVINQLTAFISGIAAISLLVGGIGIMDIMLVSVSERNREIGIRKAIGATNRQILNHFL
                     VEGLVLSIGGGIFGVVASLIVFAILRIYTNLNPVITVPIMILAVGVSVIV"
     gene            complement(1290..2168)
                     /locus_tag="VEH48_00190"
     CDS             complement(1290..2168)
                     /locus_tag="VEH48_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXY17831.1"
                     /translation="MPLIELQEVSKLYGFGDATTLALDEVFLSVEKGEFLAVMGPSGS
                     GKTTLMNIIGLLDRPTNGTYLLDGRNVARLRPNRQAKMRRDRIGFVFQFYNLLPRINV
                     LENVALPLAYRGITPVRRAKQASDMLERVGMRDREYFMPRHLSGGQAQRAAIARALIN
                     RPSILIADEPTGNLDSHDSKVVMELLSDIHRQGNTVLLVTHNPELTRYANRVIYMRDG
                     IIVADESKPIGRMAKGARRTYFRKRKTTDSDIVAGVSALLKDAPGKELSHTPKRRKIR
                     RPPRIIKKLVRIRKVK"
     gene            2337..2426
                     /locus_tag="VEH48_00195"
     tRNA            2337..2426
                     /locus_tag="VEH48_00195"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2371..2373,aa:Ser,seq:gga)
     gene            2527..2976
                     /locus_tag="VEH48_00200"
     CDS             2527..2976
                     /locus_tag="VEH48_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXY17832.1"
                     /translation="MDAKSDKQQITEVIKDVSMLQDLKQWERLENFFVKKPFVDNKAL
                     SGETPAIVPKKRLIDGWRREIGSYFYATRHFIRRLGVRMVSSRRAKVTTNVEHVHYVA
                     DRGDRYVWTVRGTIDYIVVKTSTGSWKISQMIFRLQDQAVRPLGLPA"
     gene            3066..3737
                     /locus_tag="VEH48_00205"
     CDS             3066..3737
                     /locus_tag="VEH48_00205"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXY17833.1"
                     /translation="MNVLIVYAHDEPASFTAAMKNLAISVLSSKGHSVVVSDLYGLGF
                     SAVAQKWDFVTTSGKHYNYMLEQRHAARLDMAFSPDILGEIEKLQVAELVLFITPIWW
                     FSVPAILKGWFDRVLAMGVAWDGGKIYEKGLLRSKQAILIAAAGGPEGYYQEKGRHKA
                     TTLQVLHPINHGTLAFCGFNVHEPFVALNVLGKDLAGRSQTLKELEFRLNNLTESPEW
                     LEFFG"
     gene            complement(3734..4303)
                     /locus_tag="VEH48_00210"
     CDS             complement(3734..4303)
                     /locus_tag="VEH48_00210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HXY17834.1"
                     /translation="MRLMIIWGSSRQGRNGGVVADWVKRHAAEDSRFEVDFVDLRELN
                     LPFFDEPISPFSMAGNNTDYLHPEGKAWAQRVAEAGGVIIVTPEYNHGPTGVLKNALD
                     WVGPEWGDKPVAFVSYGGAAGGARAVEQLRSITVELGLVQVANAIHFVYYRKAFNEQG
                     EPLREETNESLKKMFDEVVRLQEIFNRAG"
     gene            4352..5017
                     /locus_tag="VEH48_00215"
     CDS             4352..5017
                     /locus_tag="VEH48_00215"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HXY17835.1"
                     /translation="MLVLRETEKRYLQAMGLLSGLCLAFFVVRVILTDSSSFDFIPGN
                     LVLAWLGLVFGWLLIRQLKVTRWASWQNISLSVLWLFFLPNTWYMLTDFIHVYPTGEI
                     SELYDIAMIGSLVITGFILGFTSLYLVHKELFKRFGAKPSAYLVAGVILLSSFAIYLG
                     RDLRWNSWDVIANPSGLILNVSDRIIDPLGHLRAFNVTGLFFVLISVLYTAVWLVFRP
                     AKK"
     gene            5039..>5817
                     /gene="argS"
                     /locus_tag="VEH48_00220"
     CDS             5039..>5817
                     /gene="argS"
                     /locus_tag="VEH48_00220"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXY17836.1"
                     /translation="MQEIARALKDACQKLFNTEINPELSRPEEQFGDFATNAALQLGQ
                     KLGKNPREIAMALAGQLHGVAGVADVQIAGPGFINLKLTDEALAKTAFAATDLPKPLA
                     GQQILVEFGDPNPFKEMHIGHLYSYIVGDAIASLLETAGAEVKRLSYHGDVGLHVAKA
                     IWAMQRNGGSNIGQAYATGAKAYEEDQQAKDEIDGINQHVYQMDNPQINDLYSQGTQQ
                     SFAAFDKILDLLSISINKRYLETDSAKQGIEIVRQNTGKVF"
BASE COUNT         1341 a         1530 c         1595 g         1351 t
ORIGIN      
        1 acgatcacag atacgccgac cgctagaatc ataatcggca cggtgataac cggattcagg
       61 ttggtgtaga tcctgaggat ggcgaacact atcaacgaag cgacgacacc gaagataccg
      121 ccgccgatgc tcaaaaccaa gccttccacc aaaaaatggt tcagaatctg gcggttggtg
      181 gcgccgatgg cctttctgat gccgatctcc cggttgcgct cgctgacgct gaccagcatg
      241 atatccatga ttccaatacc gccgaccagt agtgaaatgg cggcgatgcc agagatgaaa
      301 gcggtcagct gattaataac tccgccggcg atgtgcagaa gctcgtattg tttaagcacg
      361 gtaaagttct gctggccctg atgattttta agcagcgcgg agttcacgtc gctgacagtc
      421 gcatccagat tgttggcgtc tttggccttg accaggatct gcagaatgtt agtctggccg
      481 ctggccagtt gttcagccgg cgtcaaaggg ataagaatag ccgagttgaa gtcggtttcg
      541 gccaccgaca gcagactgcc ggatgagcgc gccagcacgc cgctgacaac gaagttctgg
      601 ccggagatca tcagcgaatg gccgaccgga ttgatttccc cgaacagctg cagggcaacg
      661 tccgtgccga tgacagcgaa gttctggccg gagctatcat tgttaaaaaa gttaccatac
      721 tccacggttt ccggcagaac ttcgctggca tcgggactgg taccgataat gaaaaggtta
      781 tccagttctt tgccgccggc tttggcactg ctggtcacga aatccatcgg cgccacggcg
      841 ctgactgacg gcagctggct gatggtctcg gcatcgcggg cagtcagggt gctggcgcct
      901 aggaaggcca gcaggttgag actgctgata ccattgccgc cgccattgac gagcttgccg
      961 gaacgtacgg ttatgacatc gcggccgagg tggttgatct ggccgacaat ctgttgtttc
     1021 aggccttcac ccagactgac cactgtaact actgagctga tgccgatgat gatacccagc
     1081 atggtgaaga agctccgggc tttggcgcgg cccaaactgc ggacagcttg cttgaaatta
     1141 cctacccaca tgcgtgcctc cggcgccgtt taccgttagc cttttaccct ttaccttgct
     1201 tttactttta acccttaacc ctatgtcagg gtaaacagaa aacggaaaac gcagagtaaa
     1261 cggtgaaagg taaacggtga aaagagcgtt catttaacct tccttatgcg gactaatttt
     1321 ttgatgatgc ggggcgggcg gcggattttg cggcgtttcg gcgtgtggct gagttctttg
     1381 ccgggagcat ctttaagcag ggccgaaacg ccggcgacaa tatcgctgtc ggtagtcttg
     1441 cgcttgcgga agtaggtgcg gcgggcgccc ttggccatcc ggccgatagg cttggattca
     1501 tcggccacga tgatgccgtc gcgcatataa attacccggt tggcatagcg ggtaagttcc
     1561 gggttgtgtg tgaccagcag gacggtgttg ccctggcggt ggatgtcact tagaagctcc
     1621 atgaccactt tgctgtcatg gctatctaaa ttgccggtcg gttcgtcggc gataaggatg
     1681 ctgggccggt tgattagcgc ccgggcgatg gcggcgcgct gggcctggcc gccggataga
     1741 tggcgcggca tgaaatattc ccggtcgcgc atgcccacgc gctccagcat atcgctggcc
     1801 tgcttggcgc gccgcaccgg tgttataccc ctataggcca gaggcagagc cacattttcc
     1861 aagacattga tccgcggcag cagattataa aactggaaaa cgaagccgat gcggtcgcgg
     1921 cgcattttgg cctggcgatt aggccgaagc cgggcgacat tgcgaccgtc cagtagataa
     1981 gtgccgttgg tcgggcggtc cagcagcccg atgatattca tcagcgtggt tttgccgctg
     2041 ccggacggac ccataaccgc caggaattcc cctttttcca cgctcaaaaa gacttcgtcc
     2101 agcgccagcg tagtggcgtc accgaagccg taaagcttgg acacttcttg cagttcaatt
     2161 aacggcattg accgctttct gatttttaat attaaaaaag aggcgcgcca cctccttatc
     2221 tgaccctcgc gatttaagtc tctgtgttta ttagtttacg ctgtccggaa tgcttaaacc
     2281 aaaaatatta agttgtaaaa aatgaaaaag ggtcattttg ttagaataag aaggaaggag
     2341 aggtcgcata gcggtctagt gcgccgcctt ggaaaggcgg tatagaggca actctatcga
     2401 gggttcaaat ccctccctct ccgccatttt ttggtgataa aatataagta ggctggtaac
     2461 agccgattct cagttagttt ccgtacgctg agaacatcag gtaaagaacc gaaaggaaaa
     2521 taccagatgg atgccaaatc cgacaaacaa cagattaccg aggtcatcaa agatgtttct
     2581 atgcttcaag atctgaagca gtgggagcgg ctggaaaact ttttcgtcaa gaaaccgttt
     2641 gtagacaata aagccctgag cggcgaaaca ccggcgatcg tgcccaaaaa gcgtttaatt
     2701 gacggctggc gccgcgaaat cggcagctat ttttacgcca cccggcactt tatccgccgc
     2761 ttgggtgtgc gcatggtcag ttcgcgccgt gctaaggtga ctaccaacgt cgagcacgtg
     2821 cactatgtgg ccgaccgcgg agaccgttac gtctggacgg tccgcggcac cattgactac
     2881 atcgttgtca agacttccac cggcagctgg aaaatcagcc agatgatttt cagattacaa
     2941 gaccaagccg tccgccccct cggtctaccc gcctaattac ttgactagtc aagtgtttta
     3001 agattgaagg gtttctataa ccctctacca ctacgaccct aaaacccttt atactatgcg
     3061 taagcatgaa cgttttgatt gtttacgccc acgatgagcc cgccagcttt acagcggcca
     3121 tgaaaaactt agcgatatcc gtgctgagtt ccaaaggtca cagcgtggtg gtcagcgatc
     3181 tctacggcct gggcttcagc gccgtggctc aaaaatggga ttttgtgacc accagcggta
     3241 aacactacaa ctacatgctg gaacagcgcc acgccgcccg gctggacatg gccttttcgc
     3301 cggatatttt aggcgagata gagaagctgc aggtggccga gctggtcctg tttataaccc
     3361 cgatctggtg gttcagtgtg ccggcaattt taaagggctg gtttgaccgg gtgctggcca
     3421 tgggcgtggc ttgggacggc ggcaagattt atgaaaaggg gctgctgcgc agcaagcagg
     3481 ccatattgat tgccgccgcc ggtggcccgg aaggttatta ccaggaaaag ggccggcaca
     3541 aagctaccac actgcaggtt ttgcacccga taaaccacgg cacgctggct ttttgcggtt
     3601 ttaacgtcca cgagccgttc gtggcgctta atgttctagg caaagacttg gccggccgca
     3661 gccagactct taaagagcta gagttcagac taaataattt gaccgaaagt ccggagtggc
     3721 tggaattttt cggctagccg gcccggttga atatctcttg caggcgaact acttcatcaa
     3781 acattttttt cagtgattca ttggtttctt cacgcagcgg ttcaccctgt tcattgaagg
     3841 ctttgcggta ataaacaaaa tgaatggcgt tggcgacctg gaccagaccc aattcaacgg
     3901 tgatggagcg cagctgctca accgccctgg cgccgccggc cgcgccgccg tagctgacaa
     3961 acgccaccgg tttgtcgccc cactcggggc ctacccaatc cagggcgttt tttaatactc
     4021 ccgtcggtcc gtggttgtac tccggcgtga cgataatcac gcccccggct tcggccaccc
     4081 gctgtgccca ggccttgccc tcaggatgta agtagtccgt gttgttgccc gccatactga
     4141 acgggctgat aggctcgtca aaaaagggca ggttgagttc gcgcaaatcg acgaaatcca
     4201 cttcaaaacg cgagtcttcg gcggcgtgcc gtttgaccca atcggccact acgccaccat
     4261 tgcgaccctg cctggaactg ccccaaatga tcatcaatct catggttgtc atattagtat
     4321 accatctgtt ttgggatata atttaggggt catgttggtg ctcagggaga ccgagaaacg
     4381 ctacctgcag gcgatgggat tgcttagcgg tttatgcttg gcgtttttcg tggtgagggt
     4441 gatattgacc gattcttcca gctttgattt catccccggt aacttggtac tggcttggct
     4501 gggcctggtc ttcggctggc tattgatccg ccagctgaaa gtcacccgct gggccagctg
     4561 gcaaaacatt tcgctgagtg ttttgtggtt gttttttttg cccaacacct ggtatatgct
     4621 gaccgatttc atccacgttt acccaacggg cgagatcagc gaactttatg acatcgcgat
     4681 gattggcagc ctggtgataa ccggctttat tttaggcttt accagcttat atctggtgca
     4741 taaagagctg ttcaagcggt ttggtgccaa gcccagcgca tatcttgtgg cgggggtgat
     4801 cctgctttcc agtttcgcca tctacctggg gcgagattta cgctggaaca gctgggacgt
     4861 gatagctaac cccagcggtc ttatcttgaa cgtttcggac aggattatcg acccgctggg
     4921 ccacctgcgg gcctttaacg tcaccggctt gttttttgtg ttaatcagcg tgctgtacac
     4981 ggcagtgtgg ctggtattcc ggccggccaa aaaataagct tcttgatatg attagagcat
     5041 gcaggagata gcgcgggcgt taaaagacgc ctgccaaaag ttattcaata ctgaaattaa
     5101 cccggagctg agccggccgg aagagcagtt cggcgatttc gctaccaacg ccgcgctgca
     5161 gctcggccaa aaactcggca agaatccgcg cgaaatcgcc atggcgctgg ccggccagct
     5221 gcacggggtg gccggtgtgg ccgatgtcca aatcgccgga ccgggcttta taaacctgaa
     5281 attaacagat gaagccctgg ccaaaacggc ctttgcggcc accgacttgc ccaaaccgct
     5341 ggccggccag caaatcctgg tggagttcgg cgaccctaac cccttcaaag agatgcacat
     5401 cggccatctt tacagctata tcgtcggcga cgctatcgcc agtctgttag agaccgccgg
     5461 tgccgaggtc aaacgcctca gctaccacgg cgacgtcggc ctgcacgtcg ccaaagccat
     5521 ctgggcaatg cagcggaacg gcggcagcaa tatcggccag gcctacgcca ccggcgccaa
     5581 agcttatgag gaagaccaac aggctaaaga tgagatagac ggcatcaatc agcatgttta
     5641 ccagatggat aatccgcaaa taaatgactt atatagccag ggaacccagc aaagtttcgc
     5701 cgcctttgat aaaatacttg acttgttaag tatttctata aataagcgct atcttgaaac
     5761 tgattctgcc aagcagggaa tagaaatagt ccggcagaat accggcaagg tcttcaa
//
LOCUS       DASPTF010000009         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_589579, whole genome shotgun sequence.
ACCESSION   DASPTF010000009 DASPTF010000000
VERSION     DASPTF010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589579"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VEH48_00225"
     CDS             complement(<1..488)
                     /locus_tag="VEH48_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HXY17837.1"
                     /translation="MAKKRKPGRPPKKHAELPKPSANFWRGSGAVLMIVAGIVLAFGA
                     IISAPLPHNLWNGVWWAFGAATFLIPFALVYLGALKFVGEDQQIPLAKLAGTIGLTVF
                     LAGVLHTTFLHTDAATGNLIGGHGGQVGQAIGNVLTHAMGRFLASLVFLILAIFAVFF
                     TFG"
     gene            complement(561..965)
                     /locus_tag="VEH48_00230"
     CDS             complement(561..965)
                     /locus_tag="VEH48_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17838.1"
                     /translation="MSNLSPELYWQELSELDHRTTPYGLDLTLRDVPSEDQLGILAMD
                     AEGVTEIIMDTSAALEAYSHPSVTPGLETAQSEINPWDVALDSINGLPERVRTATMED
                     VKRVARGKRIGPVVLLTAAEVQIALHRSQLQG"
     gene            complement(1122..>2286)
                     /locus_tag="VEH48_00235"
     CDS             complement(1122..>2286)
                     /locus_tag="VEH48_00235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HXY17839.1"
                     /translation="RIFVGLFSTNMNRVQMIINAAVHHGKKVAMDGRSMVSTLEMAVR
                     NGFMKIPKGTFVPIASVATMKADEVVVVSTGCQGEPNSALVRMAMGDHKHVQLREQDT
                     VIISATPIPYSGNDALVRAMVDDMLRKGVHVYRHESHDIDHVGPLHVSGHASQEEFRE
                     MIEMLKPKFFLPIYGDYTAKKYHIDLAIEVGLPRASTLNAENGQVILISENKMELDGE
                     VPHGTILVDQTGAIVSTVVVKDRVLLAEEGLVAVVLTVDKKSGQLLTSPDIISRGFIY
                     MKDNEELMNAFRAELRRAVTQRFKRVDLDRFKAELKDYVTHFLYEQTQRSPIVIPVVN
                     VIGGRTEKGNNVMHKPAANGQPVAKTPEEIALEQQRRFAQMRQRLLNQDARVD"
BASE COUNT          504 a          623 c          595 g          564 t
ORIGIN      
        1 ccaaaagtga agaaaaccgc gaaaatcgcc agaatcagga ataccagcga ggccaagaac
       61 cggcccatgg cgtgggtcaa aacgttgcca atggcttgcc caacctgacc gccgtggccg
      121 ccaatcagat tgccggtggc ggcgtcggta tgcaaaaagg tggtgtgcag cacgccggct
      181 aaaaataccg tcaaaccgat ggtaccggcc agcttggcca gcggaatctg ctggtcctcg
      241 ccgacgaatt tcagagcgcc cagatagact aaagcgaaag gtatcagaaa agtcgccgcg
      301 ccaaaagccc accagacgcc gttccagagg ttgtgcggca gcggcgctga aataatagcg
      361 ccaaaggcca gcacgatgcc ggccacgatc atcaagacgg cgccgctgcc gcgccagaaa
      421 ttggccgacg gcttgggcag ttcagcgtgt tttttgggcg gccgcccggg tttgcgtttt
      481 ttagccatta cagattgatt ataccttagc actaactcta aagttagtgt tgtaattata
      541 acacctttat gcttagtctg tcaaccttgg agttgagacc tatggagcgc tatctgaact
      601 tctgctgcgg ttaacaggac tactggccca attctttttc ctcgagcaac tcttttgaca
      661 tcttccattg tagcagtacg aaccctttca ggtagaccgt ttatagagtc tagagctaca
      721 tcccaagggt taatttcaga ttgagctgtt tccaaaccag gggttacact aggatgagag
      781 taggcttcca aagcggcaga ggtatccatg ataatctcag ttacaccctc agcatccata
      841 gctaaaatac ctaattgatc ttcggaagga acatctctaa gtgttaggtc aagtccgtaa
      901 ggtgtcgttc tgtggtctaa ttcgcttagt tcttgccaat ataattctgg agataaatta
      961 ctcattgtgt gcccttttgt tttcagtctt tattttcacc ctaaggatta cccttatgct
     1021 gactgaataa catccgttat acgcccgctg gcccgccaaa gtcaagctcc atgccaaaat
     1081 acttgactag tcaagtattt taaatctctg ttgggcgagc gttagtcaac ccgggcatcc
     1141 tggttcagca ggcgctggcg catttgggcg aagcgacgtt gctgctccag ggcgatttct
     1201 tccggtgttt tggcgactgg ctggccgtta gcggccggct tgtgcatcac gttgttgccc
     1261 ttttctgtcc ggccgccaat cacattgact accggaatga cgatcgggct gcgctgggtc
     1321 tgctcgtaca aaaagtgcgt gacgtaatct ttcagctcgg ctttgaagcg gtccagatcc
     1381 acgcgcttaa agcgctgggt cacggcccgg cgcagttcgg cgcggaaagc gttcatcagt
     1441 tcctcgttgt ccttcatata gatgaagccg cggctgatga tgtccggact ggtcagcagc
     1501 tggccggatt tcttatccac cgttaagacc acggccacca agccttcctc ggccagcagc
     1561 acccggtctt tgaccaccac ggtgctgaca atggcgccgg tctggtctac cagaatcgtt
     1621 ccgtgcggca cttcgccgtc caactccatc ttgttctcgc tgatcaagat tacctgacca
     1681 ttttccgcgt taagcgtgct ggctcttggc aagccgacct caatcgccaa atcgatgtgg
     1741 tatttcttgg cggtgtagtc gccgtagatt ggcaaaaaga acttgggctt gagcatctca
     1801 atcatctcac ggaactcttc ctggctggcg tggccggaaa cgtgcagcgg gccgacatgg
     1861 tcaatatcgt gcgactcatg ccggtaaaca tgcacgccct tgcgcagcat atcgtctacc
     1921 atagctctga ccaaagcgtc gttgccgcta taggggatag gtgtggccga aattatcacc
     1981 gtatcctgtt ctctcagctg gacgtgcttg tgatcgccca tggccatgcg caccagcgcc
     2041 gaattgggct cgccctggca gcccgtagag accactacca cttcatcggc tttcatggtg
     2101 gccacgctgg ctatcggcac aaaggtccct ttggggattt tcatgaaacc gttgcggacg
     2161 gccatttcca gcgtactaac catgctgcgg ccgtccatgg cgactttttt gccatggtgc
     2221 acggcggcgt tgatgatcat ctgcacgcgg ttcatgttgg tgctgaacag tccgacaaag
     2281 atgcga
//
LOCUS       DASPTF010000010         3571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1162857, whole genome shotgun sequence.
ACCESSION   DASPTF010000010 DASPTF010000000
VERSION     DASPTF010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162857"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..882)
                     /locus_tag="VEH48_00240"
     CDS             complement(283..882)
                     /locus_tag="VEH48_00240"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HXY17840.1"
                     /translation="MFKLPDLGYAYDALEPYIDAKTMEIHHDKHHGAYVDKLNAALEG
                     HGDLQSDTIEEILANLNDVPEDIRTAVRNNGGGHANHTMFWETMKPGGAKEPAGELAG
                     AIKSTFGSFNDFKAKLAEAGTTRFGSGWAWLVKNGDKLEVYSTANQDSPLSEGKTPIL
                     GLDVWEHAYYLKYQNKRPDYIEAWWNVVNWDIVASRLNT"
     gene            complement(919..1566)
                     /gene="trmD"
                     /locus_tag="VEH48_00245"
     CDS             complement(919..1566)
                     /gene="trmD"
                     /locus_tag="VEH48_00245"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXY17841.1"
                     /translation="MKIQIITLFPEMFEGVLNSSMLWKAQKERLVEYKLINLRDFGIG
                     PRKTVDDTPYGGGDGMVLRPEPLFAAVEKAKKNDPEARVLLMTPRGELFDQEMAREFA
                     ATEAGLIIICGRYEGYDERITTLVDKQISVGDYVLTGGELPAMMITDAVTRLIPGVLG
                     GATSAEKESHSEAGVIEHPHYTRPEIFEGLKVPQVLLSGDHAAIAKWRAAQSKKP"
     gene            1590..1853
                     /locus_tag="VEH48_00250"
     CDS             1590..1853
                     /locus_tag="VEH48_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17842.1"
                     /translation="MERDQSGRLVLIDDTDVLNSYEVLPNSDDLEEQELLELLSGEVH
                     AFPIVLSAVQSEILMKRIQETASNPTESPSIIASAQELLDSLG"
     gene            complement(2081..2560)
                     /locus_tag="VEH48_00255"
     CDS             complement(2081..2560)
                     /locus_tag="VEH48_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HXY17843.1"
                     /translation="MAASIDQQFIEYIIKSIVNKPDAVKIDRRVDEKGVLLELTVDQE
                     DLGRVIGKRGATAQSLRTLLRALGTKNDVRYNLKIIDVGPPPAKRDDSATVAADQPVK
                     TDEPLAKAPVKDDQAAKPADDIDITEDEELPEAPSVDHSSVIDKTRKELAELDDLDV"
     gene            complement(2610..3167)
                     /gene="rpsP"
                     /locus_tag="VEH48_00260"
     CDS             complement(2610..3167)
                     /gene="rpsP"
                     /locus_tag="VEH48_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HXY17844.1"
                     /translation="MLAIRLQRTGRSGHAQFRVIVQDDRFSPKSGKVVNYVGSYNPHT
                     KETKLNGEKIIQYLDNGAQPSERVAQLLKKEGIKLPAWVKVGEKQKRAIRHPEKLRRN
                     RPAGAPAPVAEAAEEQPAVAVPTETPSETASPAEAPTEKPSAEEAAAEEVAAKEPVAE
                     EKPETPAPEPTAQADQSAEFDGPAA"
     gene            complement(3210..>3571)
                     /locus_tag="VEH48_00265"
     CDS             complement(3210..>3571)
                     /locus_tag="VEH48_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HXY17845.1"
                     /translation="ERARQQILANCFEAVIGALYLDRGYQAAQKYIQKTLLPSLDDIL
                     HSGSWMDPKSRLQETAQSREGFTPVYKVLKEEGPDHDKTFVVGVYVDNKLRGEGTGAS
                     KQAAQVAAAEAALKDYR"
BASE COUNT          696 a          957 c          945 g          973 t
ORIGIN      
        1 gacccacggt gccaaactac tgatgggctt gcaggctttt atttcacttt tgactgtcgt
       61 cctagtaacc gcccgcgccg tcaacatcct cggctaggcc tgcgccgcct tcagcctgcc
      121 tgctagcaga gccagcagct ccgctgaagg cttttggcgc attacggcgt cagcggctcc
      181 agtactcctt caccgtactt ctctgtacgg cttagtccgt acttcgccgc tttcttgtac
      241 tgcatccaaa cgcaggccta gccgcaataa atacttgact tgttaagtat ttaggcgaga
      301 agcaacaatg tcccagttga cgacgttcca ccaggcttca atgtagtctg gccttttgtt
      361 ctggtatttc aggtagtaag cgtgctccca gacgtccagg cccagtatcg gcgtcttgcc
      421 ttccgatagc gggctatcct ggttggcggt ggaataaacc tccagcttgt cgccgttttt
      481 gaccagccag gcccagccgg aaccaaagcg cgtagtgcca gcctcggcca acttggcctt
      541 aaaatcatta aagctgccga atgtgctttt gatagcccct gccaattcgc cggctggttc
      601 tttggcgcca ccgggtttca tggtctccca aaacatcgta tggttggcgt ggcctccacc
      661 gttattgcgc acagctgtac ggatatcttc aggcacgtca ttcaggttgg ccagtatctc
      721 ttctatggta tcgctttgca agtccccgtg cccctccagc gcggcgttga gtttgtctac
      781 gtaggcgccg tggtgtttgt catggtggat ttccatggtt ttggcgtcaa tatacggttc
      841 cagcgcgtca taggcgtagc ctaaatccgg taatttaaac atctctgttc cctcgtttaa
      901 tctatatcta taataacact acggtttttt ggactgggcg gcacgccatt tggcgattgc
      961 cgcgtggtca ccgctaagca ggacctgcgg aactttcaag ccttcaaaga tttccggccg
     1021 ggtatagtgc gggtgttcaa tgacgccggc ttccgaatga ctttctttct cggcgctggt
     1081 ggctccaccc aaaactcccg ggatgaggcg cgtgacggcg tcggtgatca tcatggccgg
     1141 cagctcgccg ccagtcagca cgtagtcgcc gacggaaatt tgtttgtcaa ctagggtagt
     1201 gatgcgttcg tcgtagcctt cgtagcggcc gcagattatg attaatccgg cttcggttgc
     1261 ggcgaattcg cgggccattt cctggtcaaa aagttcgccg cggggagtca ttaacaaaac
     1321 ccgggcttcg gggtcgtttt ttttagcctt ttccacagcg gcgaacagcg gctccggccg
     1381 cagcaccatg ccgtcaccgc cgccataagg cgtatcatct acggttttac gcggtccgat
     1441 gccaaaatcc cgcagattta tcaatttata ttcaactaat ctttctttct gcgctttcca
     1501 taacatactg gaattcagca cgccttcaaa catctccgga aacaaagtaa tgatttgaat
     1561 tttcatccta ttaatgatat caaataaaca tggaaagaga tcagtcgggt agattagttc
     1621 ttattgatga tacggatgtt ctgaacagct acgaggttct tcctaattcc gatgatctgg
     1681 aggaacaaga attattagag ttacttagcg gagaggttca tgcgtttcct attgttctca
     1741 gcgccgttca gtcagaaatt ctcatgaagc gaattcaaga aaccgcgagc aatcctacgg
     1801 agtcgccttc cataatagca tcggctcagg aattgctaga tagcttgggc taagatattg
     1861 accgctgctg gggggtgcag cgggagaaat gcgagtctct ggttcttgcg aacatccagg
     1921 agccctgatt agatgttgta caaaataggc gcccttgggt gtgagagttg tttgatgagc
     1981 aaatatgcta ggagcatatt tgcgaggagt tcttgagcaa gcccgttttg cactaacagc
     2041 tactcacggc gacgcagtgt tcgcaaaaac cagagacgag tcagacatcg agatcgtcga
     2101 gttcggccag ttccttgcga gtcttgtcaa ttacgctgga atggtccaca gacggcgcct
     2161 ccggcagctc ctcatcctcg gtgatatcga tatcgtcagc gggtttcgcc gcctggtcgt
     2221 ctttgaccgg agccttcgct agcggttcgt cggttttgac cggctggtca gcggccacgg
     2281 tggcgctgtc atcgcgcttg gccggcggcg ggccgacatc aataattttc aggttgtagc
     2341 ggacgtcgtt cttggtgccc agcgcgcgca gcaaagtgcg cagactttgg gcagtggcgc
     2401 cgcgcttgcc aattacccgc cccagatctt cctggtcaac ggtcagctcc agtaaaacgc
     2461 ctttttcgtc tacccggcga tcaattttta cagcgtctgg cttattgact attgatttga
     2521 tgatatattc tatgaattgc tggtctatac tcgcggccat tgcggtctcc tcacctttca
     2581 gtaatttggt agtttaatta tagcagtttt caggccgcag ggccgtcgaa ctcagcggat
     2641 tggtcagcct gggccgttgg ctccggcgcc ggggtctcgg gtttttcttc tgctaccggt
     2701 tctttagctg cgacttcctc tgccgctgcc tcttctgcag aaggtttttc ggttggagct
     2761 tcagctgggg atgcggtttc agatggagtt tcggttggca cggcgaccgc cggctgttct
     2821 tcggcggctt cagcaactgg agccggtgcg cctgccggac ggttacggcg taatttttcc
     2881 gggtggcgga tggcccgttt ctgtttttca cccactttga cccatgccgg caatttaata
     2941 ccctcttttt taagcagttg ggcgactcgt tctgaaggct gggcaccatt atctaaatat
     3001 tgaatgattt tttcgccgtt cagcttggtc tctttggtgt gcgggttgta gctgcccacg
     3061 taattcacca ctttgccgct cttggggctg aaacggtcgt cttgaacaat cacccggaat
     3121 tgggcgtggc cgctgcggcc ggtcctctgt aagcggatag ctagcatgtc ttggtcattt
     3181 tacagatata aatgcgttcc gtcaaggtgt tagcggtagt ccttcaaggc ggcttcggcg
     3241 gcggccacct gggcggcctg cttgctggcg ccagtgcctt caccgcgcaa tttattgtcc
     3301 acgtaaacgc ccaccacgaa agtcttgtcg tgatccggcc cttcttcttt caacacctta
     3361 taaaccggtg taaagccttc gcggctttgg gcggtttcct gcaggcgcga tttaggatcc
     3421 atccacgagc cgctgtgcaa gatatcgtcc aggcttggca acagagtttt ttggatatat
     3481 ttctgagccg cctgatagcc tctatccaga tacagggcgc caatcaccgc ctcaaaacaa
     3541 ttggccagaa tctgctggcg ggcgcgctcg c
//
LOCUS       DASPTF010000011         3528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1449281, whole genome shotgun sequence.
ACCESSION   DASPTF010000011 DASPTF010000000
VERSION     DASPTF010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449281"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VEH48_00270"
     CDS             complement(<1..483)
                     /locus_tag="VEH48_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HXY17846.1"
                     /translation="MPELPEVETVRTGLAKLLPGLVIKDVWDDWAKSFPNAPADVARF
                     MVGASVSGVRRRAKVLIINLSSGYSLVIHLKMTGQLVFVGRQRFGAGHPTDSLINKLP
                     DKSTRVIIDFTDGSQLFFNDQRKFGWMRLLPTIEVPEIDFLKTVGPEPLEDDFTVDRF
                     I"
     gene            complement(509..1126)
                     /locus_tag="VEH48_00275"
     CDS             complement(509..1126)
                     /locus_tag="VEH48_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17847.1"
                     /translation="MATPAEHLHGSPDFVSGEQARESVPYLRSVPDEELELPVKERDP
                     IRIDADATQVIKLENPDNEGNTHVFRRDGNLIGAAEVSVESKNIDPDLQIQTARIHFL
                     GTDPSNYKSVYLALLENVIKSDHLINQVEGTEGTAMTLAAADLSLGGYLPPGWSCEYT
                     LIGQFSSFNKGYSDDEATTFDPRYMLAHVNRGQPVEHSFKRTYEL"
     gene            1193..1432
                     /locus_tag="VEH48_00280"
     CDS             1193..1432
                     /locus_tag="VEH48_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17848.1"
                     /translation="MDSLQDILADKKFAPPDEMTAVKDYVLRHYNRPSTVKLERGALI
                     LSVRGSALAAMLQLEREKIIEACGLKQKLVIRNGS"
     gene            complement(1409..1993)
                     /locus_tag="VEH48_00285"
     CDS             complement(1409..1993)
                     /locus_tag="VEH48_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXY17849.1"
                     /translation="MIKAVIFDFFGVLATEGVTSFNKDFFAGDKEKLKQIDQLVDKLN
                     MATLGYNDFVAKVAQLAGVNEQIVREYMDVNHPNRPLLDYIRTALKPHYKLGIISNAG
                     GNWVEEILGNNDAVMFDDVVLSHKVGFAKPQAEIYEIALDKLGLKAAECVFIDDIERY
                     CDAARGLGMRVIHYQDFEQMKTELEKILATVADN"
     gene            complement(1990..2874)
                     /locus_tag="VEH48_00290"
     CDS             complement(1990..2874)
                     /locus_tag="VEH48_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXY17850.1"
                     /translation="MTDSKGEVLQDDENFRLEKIDWHGKPAIKKSVKDTTPPSRTVRI
                     QNDVLGMKFFAGLSAANPKLEIYVPEVYSLNSGFYVREYIESEPLLEKTATLDEAKPR
                     LDKLARLLADIDRIPPGREVGYVGSSNYRNLEQSIPRWADENVCDKLITDEQAKKVKL
                     ISKGLGKYLKPRIAHGDMSAYKHSYLMADGKIALIDFENFTSGAAGYFDVAWTYTRLY
                     SFASSIDVPKYFLSSFIDKAAPADHRSEQLMAVLIQRTLGMQKDAEGDAKKGMEYRSR
                     AAELLELVLQNKLELLHS"
     gene            complement(2893..3486)
                     /locus_tag="VEH48_00295"
     CDS             complement(2893..3486)
                     /locus_tag="VEH48_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17851.1"
                     /translation="MRRDALLFIGIFILVILGFLLIITSGHGKKPAAPKTTAPAVLAL
                     PDYATTDATTSMTIDGTINGDELHRAIRITIGNDSRELQIIQGYSGHVLSTQSFYDTQ
                     DAYGVFLRAINNTGFLTKLKNSTAPSDERGQCPQGNRYIFELDQNGTTLSRLWASDCG
                     AAVGTLGGNSSLLQSIFELQIPGYQNLVQNVNLAASQ"
BASE COUNT          840 a          873 c          869 g          946 t
ORIGIN      
        1 tatgaagcga tcaacggtaa agtcgtcctc cagcggctct ggcccaacgg ttttcagaaa
       61 atcaatctct ggcacctcga tagttggcaa taggcgcatc cagccgaatt tgcgctggtc
      121 gttgaaaaac agctggctgc catctgtaaa atctattatt acgcgagtgc ttttgtctgg
      181 caatttgttg atgagcgaat ccgtgggatg tcccgcgcca aagcgctgcc ggcccacaaa
      241 caccagctgt ccggtcattt taagatgaat caccaagctg tagccgctgg aaagattaat
      301 aatcagcact ttggcgcggc gccggacacc ggaaacagag gcaccgacca taaaccgggc
      361 cacgtccgcc ggcgcgttgg gaaaactttt ggcccaatca tcccacacat ctttaataac
      421 caggcccggc aacaacttcg ccaagccagt ccttacggtc tccacttctg gcaactccgg
      481 cataccccaa ttatacggcc agaaccgttt atagttcgta ggttcttttg aaagagtgtt
      541 ctaccggctg acctctgttt acatgcgcca gcatataccg ggggtcaaag gttgtagctt
      601 cgtcatctga gtaacctttg ttgaaagagc tgaactggcc aattagcgta tattcgcaag
      661 accagccggg cggcaaataa ccgccaaggc ttaagtcggc tgccgccaag gtcatggcag
      721 taccttcagt cccctccacc tgatttataa ggtggtcgct ttttataaca ttttcaagaa
      781 gcgctagata aacggacttg taattggatg ggtctgtacc taagaaatgt atcctggcgg
      841 tttgaatctg taagtccgga tcaatgtttt tgctttcaac tgatacttcg gcggcaccga
      901 tcaaattccc gtcgcgtcta aacacgtgtg tgttaccttc gttgtccgga ttttctaatt
      961 ttataacttg agttgcatcg gcatctatac gtataggatc tcgttccttg accggcaact
     1021 caagttcttc atcagggaca gaccttagat agggtacaga ttctctagct tgttcaccag
     1081 agacaaaatc tggggaaccg tgtaagtgtt cagctggagt tgccattttt gtgttagctt
     1141 atattgttaa cgttacgccc gaagatccaa tttggcaagc tatactggat atatggattc
     1201 gctgcaggac atattggcag ataaaaaatt tgcgccgccg gacgagatga ctgccgtcaa
     1261 agattacgtt ttgcgacatt ataaccggcc gtcaaccgta aagttagagc gcggcgcgct
     1321 tattttaagc gtccgcggtt cagcgctggc cgccatgctc cagttggaac gcgaaaaaat
     1381 cattgaagcc tgtggtctta aacagaaatt agttatccgc aacggtagct aggatttttt
     1441 ccagctcagt tttcatctgt tcgaaatcct ggtaatgaat aactcgcatt cccaatcccc
     1501 gcgcagcatc gcaatacctt tcaatatcgt ctataaaaac acactcagcc gctttgagcc
     1561 ctaacttatc caatgcaatt tcatagattt cggcttgggg tttggcgaac cccaccttat
     1621 gcgacaatac cacgtcatcg aacatgacag catcattatt gcccaaaatt tcttctaccc
     1681 agtttccgcc ggcattggat attattccca gtttgtaatg cggcttcagc gcagtcctta
     1741 tgtaatctaa caagggtcgg ttgggatggt ttacatccat gtattctctg actatttgct
     1801 cgttgacgcc agccagttgc gcgactttgg caacaaaatc gttgtaccca agagtagcca
     1861 tattaagttt gtctacaagt tggtcgattt gcttgagctt ttctttatcg ccggcgaaga
     1921 agtctttgtt aaagctggta acgccctcgg tggccagtac gccgaagaag tcaaaaataa
     1981 cggctttgat catgagtgca gcagctccaa tttgttttgc agcaccagct ccaaaagttc
     2041 cgcggcccgg ctacggtact ccattccctt tttagcgtcg ccctcggcgt ctttttgcat
     2101 accgagtgtc cgttgaatta agaccgccat taactgttct gaccgatggt cggctggggc
     2161 ggctttatcg ataaaagaag ataaaaagta tttagggaca tcaattgagg acgcaaagct
     2221 gtaaagccgc gtatatgtcc aagcgacatc aaaatagccg gccgcgccag aggtaaagtt
     2281 ttcaaaatca atcagtgcga tcttaccgtc agccattaaa taactgtgct tgtaagccga
     2341 catgtcgccg tgggcaatcc gcggcttcag gtacttgccg agccccttgg agattaactt
     2401 tactttttta gcctgttcat cagttataag tttgtcgcaa acattctcat ctgcccaacg
     2461 cgggatactt tgttccaggt tgcggtaatt agatgacccc acatagccga cttcccggcc
     2521 gggtggaatg cggtctatat ccgccaatag ccgggccagc ttatccagtc taggcttggc
     2581 ctcatctaaa gtcgcggttt tttcgagcag gggctcgctc tctatgtatt cacggacgta
     2641 aaaaccggag tttaaactat agacttccgg aacgtagatc tcaagtttcg ggttggcagc
     2701 agaaaggccg gcaaaaaatt tcatacccag aacgtcattt tgtatccgta cagtccgact
     2761 gggtggagtc gtgtctttga cgcttttttt gattgcgggt ttgccgtgcc aatcaatttt
     2821 ttccaggcgg aaattctcgt cgtcttgcag gacctcgcct tttgaatcag tcatcaactt
     2881 tatgataaca ggctattggc tggcggctag attaacgttt tggaccaggt tctggtagcc
     2941 cgggatctgg agttcaaata tgctttgcaa cagggaggaa ttaccgccca gcgtgccaac
     3001 ggcggcgccg cagtccgagg cccacagccg cgacagcgtg gtgccgtttt ggtccagctc
     3061 aaagatgtag cggttgcctt gcgggcattg gccgcgctcg tcactgggcg ccgtgctatt
     3121 tttgagcttg gtcaaaaaac cggtgttgtt gatggcgcgc aagaagacgc cgtaagcgtc
     3181 ctgggtgtca taaaagctct gggtgctgag cacgtggccg ctatagccct ggattatctg
     3241 taattcccgg ctgtcgttgc cgatggtgat gcggatggcg cggtgcagct cgtcgccgtt
     3301 aatggtgccg tcgatggtca ttgaggtggt ggcatcggta gtggcgtaat cgggcagtgc
     3361 caataccgcc ggcgcggtag tttttggcgc agcgggcttt ttgccgtggc cggaggtgat
     3421 aatcagcaag aaccccaata taacgaggat aaatataccg atgaaaagaa gtgcgtcgcg
     3481 tctcatggct caaatctctt ttccgagcct acaatatcat aatactga
//
LOCUS       DASPTF010000012         3201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_877083, whole genome shotgun sequence.
ACCESSION   DASPTF010000012 DASPTF010000000
VERSION     DASPTF010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877083"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VEH48_00300"
     CDS             complement(<1..344)
                     /locus_tag="VEH48_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17852.1"
                     /translation="MPLTIPELDKKWKTESEKYRTHEVGSGTQKFVKDVLESNNLFSL
                     SQGKLSTSLAKRKNEFTEESRAKAGRKADVLIYIDQDIIIPVEIEKYGHIEAGEEQLK
                     NYQTDFDKQYGIL"
     gene            complement(399..1364)
                     /locus_tag="VEH48_00305"
     CDS             complement(399..1364)
                     /locus_tag="VEH48_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HXY17853.1"
                     /translation="MPKYRTAESVSPKHPDKLCDQISDAILDAYLGVDPRARVAVETI
                     GGHGHVFVVGEVTTHNHNIDIEPIVRRLAGSEVDFRSKIVRQSPEIASGVDTGGAGDQ
                     GIMVGYATAETPEMLPEEVILARQLNQHLYAIWPNDGKTQVTLKDGKISAVVASFQHS
                     KTDELKAAVNNWLKDKPENHQIKIYTNPTGDWHQGGFEADTGLTGRKLVIDNYGPQIP
                     VGGGAFSGKDASKVDRSGAYMARRIAVDYLQKFKAKEVFCYLAYAIGVAEPLEATVIV
                     DGRPHTICGYDLTPKGIIDYLDLLKPQYEPTAQWGHFGHGFSWDK"
     regulatory      complement(1365..1462)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1533..1970
                     /locus_tag="VEH48_00310"
     CDS             1533..1970
                     /locus_tag="VEH48_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HXY17854.1"
                     /translation="MNITSPAFADNQPIPARYSCKGDNTSPPLAIAGVPPAAKSLALI
                     MHDPDAPSGDYVHWVVWNMLPSTTEIAPNSLPTSAEQGPNTSGRNSYMGPCPPSGTHR
                     YIFELYALSEKLQLAPSTGREQLVAAMDGRILAKATLTGLFSH"
     gene            complement(1989..2345)
                     /locus_tag="VEH48_00315"
     CDS             complement(1989..2345)
                     /locus_tag="VEH48_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17855.1"
                     /translation="MPAEIYCYDAATDHIFAVGNIESDGQCRVGVIAGKPESMAVICS
                     EQDDYTNVVLVTTDELLGKNTGSFSEASPKDRAARSRVVAQIAMNESRKFIFSNGYGQ
                     RVVLVRHGPRQNRELN"
     gene            complement(2401..2793)
                     /locus_tag="VEH48_00320"
     CDS             complement(2401..2793)
                     /locus_tag="VEH48_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17856.1"
                     /translation="MPAEQGYNFVQAGINFMSSNPETPESLFSKDEPVDDFVFKIGDR
                     EFSIPKQGSVIRRFEQGGGGLDYLLHATTEGTLVFMPGPEESKETFDRLVGDGFAVEY
                     LPEPDDEALRTIIELGLVDMTFPEAASA"
     gene            complement(2828..>3201)
                     /locus_tag="VEH48_00325"
     CDS             complement(2828..>3201)
                     /locus_tag="VEH48_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17857.1"
                     /translation="PFEVEHIPEGAGVRDPKRIIKLHELLRPWNFSDFSGGANGEKTG
                     SQKNLISVRRRPGENVVDIFELGNIVEFAQSKIHAVPARLYTADRIVASVGEGEEHEY
                     TRKVVDGGLTRSTVIIRYTAE"
BASE COUNT          701 a          864 c          788 g          848 t
ORIGIN      
        1 agaatgccat actgtttatc aaaatcagtc tggtaatttt ttaactgttc ttcaccagcc
       61 tcaatgtgac cgtatttctc tatttcgacc gggataataa tgtcttggtc gatgtagatg
      121 agaacatctg cttttcgccc agctttagcg cgactttcct ccgtaaattc atttttcctt
      181 ttggctaagg acgtcgagag cttaccctgt gaaagagaaa aaagattatt tgattcaaga
      241 acatccttaa cgaatttttg tgtaccgctc cctacctcat gagtgcggta tttctcagac
      301 tcagttttcc atttcttgtc tagctcagga atagtcagtg gcattcaaaa ctccaattgc
      361 acaacattat acccctgttg gatcaggttg ggaaggggtt atttatccca actaaatccg
      421 tggccgaaat gcccccactg ggcagtcggt tcatattggg gtttaagcag gtccaagtaa
      481 tctattatgc ctttgggagt cagatcgtaa ccgcagattg tatgcggccg gccgtcaact
      541 atcaccgttg cttccagcgg ctcggctacc ccgatggcat aagccaggta gcaaaaaact
      601 tctttggctt tgaatttttg taaataatca acggcgatgc gccgggccat gtaggcgccg
      661 gagcggtcca ccttggaggc gtctttgccg gaaaaggcgc cgccgccaac cgggatttgc
      721 ggcccgtaat tgtctatcac cagcttgcgg ccggtcaggc cggtgtcggc ctcaaaaccg
      781 ccttggtgcc agtcgcccgt agggttggtg taaattttta tctggtgatt ttccggctta
      841 tccttcagcc aattgttgac ggccgcttta agctcgtcgg ttttggaatg ctggaagctg
      901 gcgaccaccg cgctgatttt gccgtcttta agcgtcacct gggttttgcc gtcgttgggc
      961 cagatggcgt acaggtgctg gttgagttgg cgggccagga tgacctcttc tggcagcatt
     1021 tcgggcgttt cggcggtggc ataaccgacc atgatgcctt ggtcgccggc accgccggta
     1081 tctacgccgc tggcgatttc cgggctttgg cggacaatct tgctgcgaaa atcaacctcg
     1141 ctgccggcca agcgacggac gatcggctct atgtcaatat tgtggttgtg ggtagtaact
     1201 tcgccaacta cgaacacgtg gccgtggccg ccgatggttt ctacggccac ccgggcgcgt
     1261 gggtcaacgc ctaaataggc gtctaaaatg gcgtctgata tctgatcgca cagtttgtcc
     1321 gggtgcttgg gcgaaacact ttcggcggtt ctgtattttg gcatgaaata tccttccttt
     1381 gggctaggtt tagcacctgg ctttttaagc tggttgctgg caggtcgtcg ggctaggccc
     1441 ctggctgcac tcttgatatt taattgttaa tgaattgatt atatcttgcg agagggacag
     1501 ttgcctagat gcagggttat aattagtcag agatgaatat tactagtccg gcgtttgccg
     1561 acaaccagcc gattccggcc cgctacagct gcaagggcga taacacttcg ccgccgctgg
     1621 ctatcgccgg tgtgccgccg gcggccaaga gcctggcgct gataatgcac gacccggacg
     1681 cgcccagcgg cgattacgtg cactgggttg tctggaacat gctgccctca acaaccgaaa
     1741 ttgcgccaaa tagcctgccg accagtgcag agcaggggcc aaacaccagc ggccgcaact
     1801 cctacatggg cccgtgcccg ccttccggca cccaccgcta catttttgaa ctttacgcgc
     1861 tgagtgaaaa gctgcagctg gcgccaagca ccggccgcga acagcttgtc gcggccatgg
     1921 acggccgtat cctagccaaa gccaccctga ccggcctctt cagccactag ggtcaaagga
     1981 caggccagtt aattcagctc cctattttgg cgcggtccat gtctaaccag aacaactctt
     2041 tggccataac cattgctgaa gataaatttt ctagactcgt tcatcgcgat ctgggcaaca
     2101 acccttgacc tcgccgccct gtctttgggt gaagcctcac taaaagagcc tgtgtttttg
     2161 cccaataatt catccgttgt caccagcact acgttcgtat aatcatcttg ctcggaacaa
     2221 attaccgcca ttgactctgg tttgcccgcg ataaccccga cccggcattg tccgtcagat
     2281 tcgatgttgc ctacggcaaa aatgtgatct gtagcggcat cgtaacaata tatttcggca
     2341 ggcatttagt tttaagtccg ttggggcaaa ttataccccc gttaaggcga ctgtgccagg
     2401 ttatgcgcta gctgcctcag ggaatgtcat atccactaat cccagttcta tgatagttcg
     2461 cagagcctcg tcgtccggtt ccggcagata ttctaccgca aatccgtccc cgaccaatcg
     2521 atcaaaggtc tccttgctct cttcaggacc aggcataaat accaatgttc cttccgtggt
     2581 tgcgtgcagc aggtaatcta atccgccacc gccttgttca aaccttctta ttacactgcc
     2641 ttgctttgga attgaaaact ctctgtcgcc gattttgaat acaaaatcgt caaccggttc
     2701 atcttttgag aatagtgact ctggtgtttc tggattgctg gacataaagt tgattcccgc
     2761 ttggacaaaa ttatacccct gttcggctgg catagccagc ggtagatatc tagtagttag
     2821 tagatgatta ctcagctgtg tagcgaataa tcacagtcga cctggtcaag ccgccatcga
     2881 caactttgcg cgtatactca tgttcttcgc cctcgccgac agacgcgact atcctgtcag
     2941 ccgtataaag cctggccggt acagcatgga tcttagattg agcgaattcc acaatattgc
     3001 ccagctcaaa aatgtctacc acattttccc ccggccggcg acggacagaa atcaggttct
     3061 tctgactgcc ggttttttcg ccattagcgc caccggaaaa atcgctaaaa ttccatggcc
     3121 gtaaaagctc gtgtagcttt ataatgcgct tcgggtctct tacgcccgcc ccttcaggaa
     3181 tatgctcaac ttcaaacggc a
//
LOCUS       DASPTF010000013         5429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_880536, whole genome shotgun sequence.
ACCESSION   DASPTF010000013 DASPTF010000000
VERSION     DASPTF010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880536"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VEH48_00330"
     CDS             <1..433
                     /locus_tag="VEH48_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17858.1"
                     /translation="IHTTSTDLEMVPIFVSFGPKSKKILNVLDSKQVVIMPKSKDSLI
                     GAFGNGAVSLEFGDSYCRSNSALHEVHEFLLKLASRKERRPKTREIYRITSLAPPKTK
                     KTACANFQYSKLLGGYGFLIGERSYKDFGGFVAQKKQKLVV"
     gene            438..1565
                     /gene="trpS"
                     /locus_tag="VEH48_00335"
     CDS             438..1565
                     /gene="trpS"
                     /locus_tag="VEH48_00335"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXY17859.1"
                     /translation="MAKEVILTGIRSNKDLHIGNYFGALLPMIDMAKQHSGEYQINLF
                     VPDLHSFTTPIDHDQLQAQTMHNLKIFVAAGLPLDNPGIHIYRQSYIPAHSEMTWILD
                     CFTGFGEMSRMTQFKDKAASLLMKGSSVTPPVKNINGEFKFDEKQAQKNDELRRDRVS
                     VGLFNYPVLMAADILLYGAKYVPVGDDQTQHLEFTRDIAERMNKQFGDLFIVPEPVAK
                     QHEFFGKDQGLRIKDLADPAKKMSKSEDSDKGVIFMGDDPEAAAQKIMSATTDSYNRL
                     PSDDEERKKQPGITNLMLIKVLLTGNLNLPESNTQGYAYLKEQVANDVKAFLSDFQAK
                     MAQVDEEAIQQKLTADEAKMTEVANKTLLKVQQAVGLRPNS"
     gene            1568..2440
                     /locus_tag="VEH48_00340"
     CDS             1568..2440
                     /locus_tag="VEH48_00340"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXY17860.1"
                     /translation="MRRFKVGQAGAGQRADVFIAAKFPRFSRSFLEYLFDAGSVKSND
                     KIIKASHKLQTGDKIKVEDGLLKIKPAKIKLPVVHEDDDVVVIDKPAGVLAHSKGALN
                     NEATVASFIRSKIKDKTMTGNRAGIVHRLDRPTSGLIITARTAAALQHLQRQFSKRKT
                     DKTYLAVVEGWPEPAEALIDAPIERNPRRPQTFRVAAGGKPAQTHYKTLRQLQKNRKK
                     YAFLELKPATGRTHQLRIHLSYLGHPIVGDSLYGHTDGQLLLHASRLEITLPSGHRKV
                     FESPAPRRIKEFIA"
     gene            2440..3321
                     /locus_tag="VEH48_00345"
     CDS             2440..3321
                     /locus_tag="VEH48_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17861.1"
                     /translation="MTAFRHELLLHPRTQEQIGAFLAHPAHALLITGVAGSGKLSVAR
                     ATAANLLGLEPSKLEVYPYYSQIQTPEGKQEISIDAIRELIDRLKLKTPGTASIRRVV
                     VIENSQAMSIEAQNSILKTLEEPSAETVFILTAPAAKNVAPTIASRAQIIGVKPVARA
                     AANDYYGGRYEQKQIDANWRLSGGAAGLLEALIKNSAEHPLKLAVDDFKKFLAADTYG
                     RLLDGERLARDKAQMAYFLDAGGRVLRALQQTEAGQKRQNAITVGRKLILSLQAALQA
                     NANPRLVAAALALNLKL"
     gene            3346..3999
                     /locus_tag="VEH48_00350"
     CDS             3346..3999
                     /locus_tag="VEH48_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXY17862.1"
                     /translation="MYSLILLAVFGLIIFRHSRLAQRDLVIIPKRIGERLSHLWEIAH
                     HGMRENRFLRAEKALLTILKIDEKNAAAYNRLGILYAKQREYKDAIDCFEIASSIEPS
                     ASSLHNLGLIYYETENYQKAATAFEQALKLEDGLAARHVAYAKVQEKLGNDKLVIQEL
                     QKAVELEPNPETYGLLAKAYESRGMTAEAAAVQKKMGRLVVPSGRPKRILRPRRVVI"
     gene            4053..4129
                     /locus_tag="VEH48_00355"
     tRNA            4053..4129
                     /locus_tag="VEH48_00355"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4087..4089,aa:Thr,seq:tgt)
     gene            4177..4584
                     /locus_tag="VEH48_00360"
     CDS             4177..4584
                     /locus_tag="VEH48_00360"
                     /inference="COORDINATES: protein motif:HMM:NF023075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group I intron-associated PD-(D/E)XK
                     endonuclease"
                     /protein_id="HXY17863.1"
                     /translation="MRPKEKGDIALAYAIQHYLLSGYEVCLPVGDKRDYDLVIERDNV
                     LERVQVKYAGFYPAKNKCLVGLRITGGNQSYHYAKKYTDTAFDYLFVYTARGRSYSLP
                     WSEVKARSELSIENVKYNKYLTSSWDTQVVNEG"
     gene            4553..4638
                     /locus_tag="VEH48_00365"
     tRNA            4553..4638
                     /locus_tag="VEH48_00365"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4587..4589,aa:Tyr,seq:gta)
     gene            4678..5118
                     /locus_tag="VEH48_00370"
     CDS             4678..5118
                     /locus_tag="VEH48_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17864.1"
                     /translation="MSEGLGNHPLRTQILSELQLENDILGITDIFRAELTKKADGKKK
                     ELEAAVLDNELTRLQRESDQVQSAIDDIAPITPVIYQYGSSLQLCLRREYDSLGLVVP
                     GSTVKIASSEVGVGEYTPEGGFTAEQVDAVLGQLARYMSFSELN"
BASE COUNT         1516 a         1338 c         1356 g         1219 t
ORIGIN      
        1 cattcacact acctcgactg acttagaaat ggtgccgatt tttgtgagtt ttggccccaa
       61 aagcaagaag attctaaacg ttttggatag taaacaagtg gtcataatgc ctaaaagcaa
      121 agactctttg attggagctt ttggcaacgg ggcggtatct ttagaatttg gcgattcata
      181 ctgccgcagc aattcggctc ttcatgaagt ccacgaattt ctgttaaagc tggcctcgcg
      241 caaagaacgg agacctaaaa cacgggaaat ttatagaata acgtctttag cgccgccaaa
      301 aacaaagaag acggcctgtg caaacttcca gtactccaaa ctgctaggag gctatggctt
      361 cttaatcggc gagcgatcct acaaagattt tggcggtttt gttgctcaaa aaaagcaaaa
      421 actggtagtc taatactatg gctaaagagg tgattttaac tggcatcagg tccaataagg
      481 atctgcatat tggcaactat tttggcgcgc tgttgcctat gattgatatg gccaaacagc
      541 actcaggcga gtaccaaatt aacctgttcg tgcccgattt gcacagcttc acaacgccga
      601 ttgaccacga ccagctgcaa gctcagacca tgcacaacct gaagatattc gtcgcagccg
      661 gtctgccgct ggacaatccc ggcatccata tctaccgtca aagctatatt cctgcccact
      721 ccgaaatgac ttggatatta gattgtttta ccggctttgg cgaaatgagc cgcatgacgc
      781 agtttaaaga taaggccgcc agtttgttga tgaaggggtc atccgttact ccaccggtca
      841 aaaatattaa cggagaattt aaatttgacg agaagcaggc gcaaaaaaat gatgaactga
      901 ggcgtgatcg agttagtgtg ggacttttta actacccagt gctgatggcg gcggatatct
      961 tgctctatgg cgccaaatac gtgccagttg gtgatgacca gacccagcat ctggagttta
     1021 cgcgcgacat tgccgaacgt atgaataagc agttcggcga tctgttcata gtgcccgaac
     1081 cggtcgccaa acagcacgaa ttctttggca aagaccaggg gctacgtatc aaagacctgg
     1141 ccgacccggc taaaaaaatg agcaagagcg aagacagcga caagggcgta atcttcatgg
     1201 gcgacgatcc cgaggcggcg gcccaaaaga tcatgagcgc tacaactgat agctataacc
     1261 gcctacccag tgatgacgaa gaacgcaaaa agcaacccgg aattaccaac ctaatgctta
     1321 tcaaggtgct gctaaccggg aatctgaact tgccggaaag caacactcaa ggctacgcat
     1381 acttaaaaga gcaagtcgcc aatgatgtta aagctttctt gtctgacttt caggccaaaa
     1441 tggctcaggt tgacgaagag gctatccagc aaaaactgac agccgacgag gctaaaatga
     1501 ccgaagttgc gaataaaacc ctcctaaaag tccaacaagc cgtcggcctc cgacccaata
     1561 gttaagtatg cgcaggttca aagtcggcca agccggggcg gggcagaggg cggacgtttt
     1621 tatagccgcc aaattcccac gtttcagtcg ctccttcttg gagtacttat ttgacgctgg
     1681 atccgttaaa tccaacgaca agataattaa agccagccac aagctgcaga caggcgataa
     1741 aattaaagtt gaagacggtc tgctaaagat caagccggct aagattaagc tgccagttgt
     1801 tcacgaagat gacgacgtgg tggtgataga taaaccggcg ggagtattgg cccacagcaa
     1861 aggtgcgctg aataacgagg cgacggtcgc cagctttatc agaagcaaaa taaaggataa
     1921 gacaatgacc ggcaatcgcg ccggcatagt gcaccggctg gaccggccga ccagcggtct
     1981 gattatcacc gcccggaccg cagcggccct acagcacctg cagcgccagt tctccaagcg
     2041 caaaactgac aaaacttacc tggcggtagt agaaggctgg ccggaaccgg ccgaagcttt
     2101 gattgacgcg ccgattgagc gcaacccgcg gcggccgcag actttcaggg tggcagccgg
     2161 cggcaaacca gcccaaactc attataagac actccggcaa ctgcaaaaaa atcgcaaaaa
     2221 atacgcattt ttagagctta aaccggccac cggccgcact catcaattgc gcattcacct
     2281 cagttatctt ggccacccga tagtcggcga cagcttatac ggacacaccg atggccaact
     2341 gctactgcat gcttcgcggt tggagataac cctccccagc ggccatagga aagtatttga
     2401 atcaccggcg ccgcggcgca tcaaggagtt catagcctga tgacggcttt tagacacgag
     2461 ctgttactgc atccacgcac ccaggaacag attggcgctt tcctagcgca cccggcccac
     2521 gccttgctga ttacgggagt ggccggtagc ggcaaactgt ctgttgccag agccaccgca
     2581 gccaatctgc tgggtttgga gccatccaag cttgaggttt atccttatta ctcccagatt
     2641 caaactccag agggcaagca ggagatatca attgacgcca tcagggagct gatagatagg
     2701 cttaagctta agacaccggg tacggctagt atcagaaggg tggtggtgat tgaaaactcc
     2761 caggctatga gtattgaggc gcaaaattcc atcttaaaga cactggaaga gccgagcgct
     2821 gagacagtgt ttatcttaac ggcgcctgcg gccaaaaacg tcgcgccgac aatcgcttcg
     2881 cgggcgcaga ttatcggcgt aaagccggtt gctcgcgccg ccgccaatga ttattacggc
     2941 ggacgttacg aacaaaagca gatagatgcc aactggcgtt tgagcggcgg cgccgccgga
     3001 ctgctggaag cgctgataaa aaacagtgcc gaacaccctc tgaagctcgc agtagacgat
     3061 tttaagaagt ttttggccgc cgacacctac ggccggctgc ttgacggtga gcgcctggcg
     3121 cgcgacaaag cgcagatggc ctattttttg gacgccggcg gccgggtact gcgcgccctg
     3181 cagcagaccg aggctggcca aaaacggcaa aacgccataa ccgtcggccg caaactcatc
     3241 ttgtcactgc aggccgcgct gcaggctaac gccaaccctc ggctggtagc cgcggccctg
     3301 gcgcttaacc ttaaactgta aaattgctat aattgatact tagtaatgta ttcattgata
     3361 ctgttggctg tgttcggctt gattatcttt cggcatagcc ggttggctca gcgggatctg
     3421 gtcatcatcc ctaaacgcat tggcgagcgg ctgtcgcatc tttgggaaat tgcccaccac
     3481 ggcatgcgcg agaaccgttt tttacgtgcc gagaaggcct tgctgaccat acttaagata
     3541 gacgagaaaa acgccgcggc ctataaccgc ctgggcatct tatacgccaa acaacgcgag
     3601 tataaagacg ccatagactg ctttgagatc gccagcagta tagaacccag cgcctccagt
     3661 ctccacaacc tcggcttgat atattatgag accgagaatt atcaaaaagc cgctaccgcg
     3721 tttgaacagg cgctgaagct ggaagatggt ttagcggccc gccacgtagc ctacgccaaa
     3781 gtccaagaga agcttggcaa cgacaaactg gtcattcagg aactgcaaaa agccgtggaa
     3841 ctggaaccca accccgagac ttacggcctg ctggccaagg cttacgagtc gcgcggcatg
     3901 accgcagaag ccgccgccgt gcaaaagaaa atgggccggt tggttgtgcc ttccggccga
     3961 cccaaacgca ttctgcgccc ccgccgggtc gtcatctagc cgccgattgc tatttcaccc
     4021 ctgttatgct aaacttttta tggtcttttt gagccgcctt agctcagttg gctagagcag
     4081 ttgctttgta agcatcgggt cgtcggttcg agtccgacag gcggctccaa gaaattgcac
     4141 cttacaaact gaactatact cagctacact ttgtctgtga gaccgaaaga aaaaggcgat
     4201 atcgcgctag cttacgcaat ccagcactat ttactgtctg gatatgaagt ttgtcttccc
     4261 gtaggtgaca agcgggacta tgatcttgtg atcgagcgtg ataatgtcct tgaacgcgta
     4321 caggtgaagt atgcaggttt ctatcctgcg aaaaacaagt gcctggttgg attaagaatt
     4381 actggcggca accaatcata ccactatgcc aagaagtaca ctgatacggc atttgactac
     4441 ctgtttgtgt atacagctag agggaggagc tattcgctgc cttggagcga agtaaaagcg
     4501 aggagtgagc ttagtataga aaatgtcaag tataataaat acttgacttc aagctgggat
     4561 actcaagtgg tcaacgaggg gtgactgtaa accatctggc attagcctac gggggttcga
     4621 atccctctcc cagcaccaga tatttacaaa taccactaaa cgtggtatat taattatatg
     4681 agcgaaggtc taggaaatca cccgctgcgt actcagatat taagtgaatt acaacttgaa
     4741 aatgacatac ttggaattac tgatatcttt cgagcagaac ttactaaaaa agcagatggc
     4801 aaaaagaagg aattagaagc tgctgtgctg gataacgaac tcacacgatt gcagcgtgag
     4861 tcagaccaag tacaaagtgc cattgatgat attgcaccga ttacccctgt aatctatcaa
     4921 tatggaagtt ctttacaact atgcttgcgt cgtgagtatg acagtcttgg cctagtagtg
     4981 ccaggatcaa ctgtaaaaat tgcttccagt gaggtaggtg ttggtgaata tactcccgag
     5041 ggtggcttta ccgcagaaca agtggacgca gtattaggcc aacttgcgcg ctacatgtcg
     5101 ttttctgaat taaactagac taatatgaag tgagcagggt tcttacttcg tagagcacgc
     5161 tgcaccacag atgagagcga ggaggacgtt agaactaaat cagttctata atcccactcc
     5221 agctcttata tatttgattt ataaaaacta ttattgtaaa atactagtta tggttgaagc
     5281 tattgatgaa aaagctcaag gtaatcctga accatttaaa tacattaccc cactagagcc
     5341 atataacgat actgttttac agtatatggt tcttgagaag atgggctacc cagtaaggct
     5401 tgaagaggtt gaccgaaagt ccttacccg
//
LOCUS       DASPTF010000014         4463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_305849, whole genome shotgun sequence.
ACCESSION   DASPTF010000014 DASPTF010000000
VERSION     DASPTF010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305849"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VEH48_00375"
     CDS             complement(<1..292)
                     /locus_tag="VEH48_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXY17865.1"
                     /translation="MNIRENVELKNYSTMRLGGQSRYLAEAGSLEDVEQLGKWAREHN
                     LPVITVGGGSNIVWKDEGFAGLVVVNKIMGRELASDGLTIKLGAGENWDNAVG"
     gene            317..868
                     /locus_tag="VEH48_00380"
     CDS             317..868
                     /locus_tag="VEH48_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXY17866.1"
                     /translation="MNEPHQSSYRTLGKRLKELRTRANETLAEASGAVEIDVRQLASY
                     EMGRKRPAEEVLLLLISHFNVRDDEAVSIWELAGYGGGRLAAAIDSQPATAVTNEKKI
                     LFTDVVDIMVNNYGVVMNFMQSSGPNNQPSSVARVGMSREHAKSVLKILQVTLAQTEK
                     QSSSLPKKIIAPDTSTPENSPQN"
     gene            904..980
                     /locus_tag="VEH48_00385"
     tRNA            904..980
                     /locus_tag="VEH48_00385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:938..940,aa:Arg,seq:ccg)
     gene            1134..1209
                     /locus_tag="VEH48_00390"
     tRNA            1134..1209
                     /locus_tag="VEH48_00390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1168..1170,aa:Arg,seq:tcg)
     gene            1255..1563
                     /locus_tag="VEH48_00395"
     CDS             1255..1563
                     /locus_tag="VEH48_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17867.1"
                     /translation="MVKAKPTKSLDQIAITVVLLEVVFGTVAYKILVRPYHGELWLRI
                     IFWPAFIISAVAFAANLKMGWALIRRKHSIKNNKVAVILFCFGLLFILMINLLYWSQT
                     "
     gene            complement(1599..2354)
                     /locus_tag="VEH48_00400"
     CDS             complement(1599..2354)
                     /locus_tag="VEH48_00400"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HXY17868.1"
                     /translation="MTVKATKTRKVTAGSGSLEQLLDESLGELEDSSIVVITSKVVSL
                     CEKRVARTAGTDKEELIKSESDLYLPKASSKYSHHFTILRNTLVASAGIDESNAGDNY
                     ALWPLDPQLSANKARAHLAQKFGLKNIGVIITDSVSSPLRFGTRGTAIAYTGFAAMHD
                     YRGELDLFGYEFRLEQANIAEGLASAAVVVMGEGAEQTPLAIITNTPFVRFQDHNPTE
                     KELKKFHVERIENDLFEPFLRQPGWRKGGHRQD"
     gene            complement(2351..2845)
                     /locus_tag="VEH48_00405"
     CDS             complement(2351..2845)
                     /locus_tag="VEH48_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HXY17869.1"
                     /translation="MTDKRELRRTIRARRLAMTVNEVETKSRVIADKLISLVDWPRVR
                     AMHIYASRAGWNEVDTAPVIRLVRKHWPAVRIVQPPPRPDLVLPEGKFDLIIIPCLGF
                     AEDRHRLGLGGGWYDRFLVRQPQALKIGLAFQNGLIESGLPREPHDIRLDKIITEEVI
                     MEAR"
     gene            2903..4216
                     /gene="hisS"
                     /locus_tag="VEH48_00410"
     CDS             2903..4216
                     /gene="hisS"
                     /locus_tag="VEH48_00410"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HXY17870.1"
                     /translation="MAVNQGPLSGFRDMLAPQAIPRQQMLDVIKVVYENYGFTPLKTP
                     ALERYSTLSGKYGEEGDKLMYRFEDQGGREVALRYDLTVPLARVVAQYGSQIPLPYKR
                     YAVGEVWRGERAQAGRFREFTQFDADTIGSKSPLADAEIVAVMSDSMAALGARSVIRV
                     NNRRILDALAGKAGITDEKAVRAFMSSIDKVEKIGLGKALAEILKNFSQKATTLASGY
                     LEVQGSSNDKLQSLVRLMGDSEAIAEGVENLSEVFKILAAAGYSEEQVVFDQAIARGL
                     DYYTGIIYETTLVGAESLGSVCGGGRYDNLVKALGGPDLPAVGTSIGVDRLFDGLQLL
                     GKLQNNKTSTQVLIANFDSADSGEYLRIAAKLRAAGIAAEIYYDPAKLGKQISFADKM
                     SIPYVVLLGSEERAKSIATIRSLDTGNQEEVPIAKLSEALRKLVK"
     gene            4262..4338
                     /locus_tag="VEH48_00415"
     tRNA            4262..4338
                     /locus_tag="VEH48_00415"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4296..4298,aa:Lys,seq:ctt)
BASE COUNT         1076 a         1229 c         1155 g         1003 t
ORIGIN      
        1 agccgactgc gttgtcccag ttttcgccgg cgccaagctt gatggtcaga ccgtcactgg
       61 ccagctcgcg gcccataatc ttgttaacga ccaccaagcc ggcaaagcct tcgtccttcc
      121 aaacgatatt gctgccgcca ccgacggtaa tcaccggcag gttgtgttcc cgggcccatt
      181 tgcccaattg ctccacatcc tctaaagatc cggcctcggc caaatagcgg gattgcccgc
      241 ccaggcgcat ggtactgtaa tttttcagct caacattttc ccggatattc actagcttat
      301 tgtacaatag gagcaaatga acgaaccgca ccagagttct taccgtacgc ttggtaaacg
      361 cttgaaagaa cttcgtaccc gggccaatga aaccttggcc gaagcctcgg gggcggtaga
      421 gattgacgtt cgccaactgg ccagttacga gatgggccgc aagcgcccgg cagaagaggt
      481 actgctgctt ttaatcagcc attttaacgt gcgtgatgac gaagccgtat caatttggga
      541 gctggccggt tacggcggcg gccgcttagc cgctgccatt gacagccagc cagcaacagc
      601 ggtaactaac gaaaagaaaa tcctttttac cgatgtcgta gacatcatgg tcaacaacta
      661 cggcgtagtg atgaacttta tgcaaagcag cgggccaaat aaccagcctt catctgtcgc
      721 ccgcgtaggc atgagccgcg aacacgctaa aagcgtacta aaaatcctgc aagtcacctt
      781 ggcccaaact gaaaaacaat cgtcaagctt acccaagaaa ataatcgcgc cagacaccag
      841 cacccctgaa aattctcccc aaaactgata taatacccct gttcttacca aatttttaaa
      901 gcggggccct tagctcagtt ggatagagcg ctagcttccg gagctagagg ccagaggttc
      961 gaatcctcta gggctcacca taactgctac aattgggctt gttctggctc tttacaattt
     1021 tgcaaagcgg ggcgctagcg accgtttgca aaattgagag gagcaagcga gccgaggctg
     1081 aaagtgagcg ctggagtaca agctcacgga agccgctgcc tcgattgcga cgagcccccg
     1141 tagctcagtg gattagagca ctggtcttcg gaaccaggcg cgcaagttcg agtcttgccg
     1201 ggggtaccaa agcataagct ttttattacg gagtggccgt tgtatcctat ctatatggta
     1261 aaggctaaac cgactaagag cttggatcaa atagccataa cagtcgtatt gcttgaagtc
     1321 gtttttggga cggttgccta taagatactt gtaaggccgt accatgggga gctctggctt
     1381 aggataatct tttggccggc atttattatc agcgccgtgg ctttcgcggc caacctcaaa
     1441 atgggctggg cgctcatcag gcgcaaacac tccataaaaa acaataaagt ggccgttatt
     1501 ttgttctgct ttggtctgtt attcatctta atgatcaatc ttctctattg gtcgcagacc
     1561 taatttctaa gtaagcgtct tctaaaaccc tcgaaaaact agtcttggcg atggccgcct
     1621 tttcgccagc ccggctggcg caggaacggt tcgaacaggt cgttttctat acgttcaacg
     1681 tggaattttt ttagctcttt ctccgtcggg ttgtgatcct gaaaccgcac gaatggcgta
     1741 ttggtaatga tagccagagg agtctgctcg gctccctcgc ccatgacgac cacagccgcc
     1801 gaagccagcc cttcggcgat gttagcttgt tccagccgga attcataacc aaaaagatct
     1861 aactccccgc ggtaatcatg catggctgca aatccggtgt aagcaatagc cgtgcccctg
     1921 gtgccaaaac gcaaagggga agaaacactg tcggttatta tcacgccgat attttttagt
     1981 ccgaattttt gagcaagatg agcccgggct ttgttagccg acaattgggg gtcaagcggc
     2041 cacaaagcat aattgtcacc ggcgttggac tcgtctattc cggcgctggc tactagggta
     2101 ttgcgcaaaa tggtgaagtg gtggctgtat ttgctggaag cctttggcag gtacaaatcc
     2161 gattcactct ttatcagctc ctctttgtcc gtgcccgctg tcctggcaac ccgcttctcg
     2221 cacagcgata caaccttgga tgttataacc acgatgctgc tgtcttccaa ttcgcccaaa
     2281 gattcatcta acagctgctc cagcgagccg gaaccagcgg tcactttgcg ggtttttgtg
     2341 gccttgactg tcatcgagcc tccattataa cttcctcggt gatgatttta tcaaggcgga
     2401 tatcgtgggg ttcgcgcggc agcccgcttt ctatcagtcc gttttgaaaa gccaggccga
     2461 tttttagggc ctggggctgc cgcaccagaa aacggtcgta ccagccgccg cccagaccca
     2521 gccggtgacg gtcttcggca aagccgagac agggtataat tatcagatca aatttgcctt
     2581 cgggtaagac caggtccggc cggggcggcg gctggactat ccgaacagcg ggccagtgct
     2641 tgcgcaccag ccggataacc ggcgcggtat ccacttcatt ccacccggcc cgcgaggcgt
     2701 aaatgtgcat tgcccgtacg cgcggccagt ccactaagct tatcaattta tcggctatca
     2761 cccggctttt agtctctacc tcattgacgg tcatcgccag ccgtctggct ctaatagttc
     2821 gccttagttc ccgcttgtcc gtcattcggt aattgtacca acagttgcta aaatgtcctt
     2881 ataggagaaa gagcttaaac ttatggctgt taaccagggc ccgctatccg gcttcaggga
     2941 tatgctcgcg ccgcaggcga ttccgcgcca gcaaatgctg gacgttatta aagtcgtcta
     3001 cgaaaattat ggctttacac ctcttaaaac acctgcgcta gagcgctatt ccaccctaag
     3061 cggtaagtac ggcgaagaag gcgataagtt gatgtacaga tttgaggacc agggcggccg
     3121 cgaagtggcg ctgcgctatg acctgacagt gccgctggca agagtcgttg cccaatacgg
     3181 cagccagata cccttgccgt acaagcgcta cgccgtcggc gaagtctggc gcggcgagcg
     3241 cgcccaggcc gggcgcttcc gcgaattcac ccagtttgac gccgacacaa taggcagtaa
     3301 aagcccgctg gccgacgccg aaatcgtggc cgtgatgtcc gacagtatgg cggccctggg
     3361 agcccggtca gtcatccgtg tcaataaccg ccgcattctg gacgccctgg ccggaaaagc
     3421 cggaataacg gatgaaaaag ccgtccgcgc ctttatgagc tccattgaca aggtcgaaaa
     3481 aatcggcctc ggcaaagccc tggccgaaat cctcaaaaac ttcagccaaa aagccactac
     3541 acttgccagc ggatatctag aagtacaggg aagttctaat gataaactcc aaagcttggt
     3601 caggctgatg ggtgattccg aggccatagc cgaaggtgtg gagaacttaa gcgaggtctt
     3661 taaaatcctg gccgccgccg gctactctga agagcaggtg gtctttgacc aggccattgc
     3721 ccgcgggctg gattattata ccggcatcat ctacgaaact acgcttgtgg gcgccgaaag
     3781 cctgggcagc gtctgcggcg gcggccgcta tgataatctg gtcaaggccc ttggcggtcc
     3841 cgatctgccg gctgtcggta cctctatcgg agtagacaga ttgtttgacg gcctccagct
     3901 gctgggcaaa ctgcaaaaca ataagaccag cacccaagtg ctgatcgcca attttgacag
     3961 cgccgacagc ggcgaatacc tgcgtatagc tgccaaactg cgggccgccg gcatcgccgc
     4021 cgaaatctat tacgacccgg ccaaactcgg taaacagata agcttcgccg acaaaatgtc
     4081 aatcccctac gtagttctac tgggatccga agaacgcgcc aaaagcatcg ccaccatccg
     4141 ctcactagac accggtaatc aggaagaagt ccctatagcc aagctttccg aagccctgcg
     4201 caaactggtc aaatagttgc aataacaggg gtagcgtgct aatatttggg ggctcaaaca
     4261 cgggccatta gctcagctgg ttagagcagc ggcctcttaa gccgcgtgtc gtgggttcga
     4321 atcccacatg gccctccaca aaactatgaa attacttaat ggctcagagt tggctggatt
     4381 tataaaagag cgccacgcca ggcaggtgcg cagcctgcgc gggcagggca ttattcccaa
     4441 actggccata gtccaggtca aag
//
LOCUS       DASPTF010000015         7231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1311334, whole genome shotgun sequence.
ACCESSION   DASPTF010000015 DASPTF010000000
VERSION     DASPTF010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311334"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VEH48_00420"
     CDS             <1..444
                     /locus_tag="VEH48_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17871.1"
                     /translation="NIDRTHFDITAHAKVQNATVTNYVFTVVNSQGQAVDTATVTTSA
                     LSAVYHFNQSAAGTYTVNVVINTDQGSTKGKCDCSVKVTVKPAPTPPVTPPVTPTPPT
                     TPPTTPKVQAATTLPNTGPGDIGAMFVGTSAFGGVGHYLVSRRKR"
     gene            complement(635..709)
                     /locus_tag="VEH48_00425"
     tRNA            complement(635..709)
                     /locus_tag="VEH48_00425"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(674..676),aa:Glu,seq:ttc)
     gene            793..2094
                     /locus_tag="VEH48_00430"
     CDS             793..2094
                     /locus_tag="VEH48_00430"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXY17872.1"
                     /translation="MFNKKTIKDIELNGQRVLLRADYNVPVENGRITDDYRMKRSLPT
                     LRFILDQPGTSIVIISHLGRPKGPEDKDLSLKPVAEHLSKLLDKEVGFVGDCIGEEAK
                     RAADSLQAGGILMFENVRFHPEEEKNDPAFAKQLADAAGAQLFVQDGFGVVHRAHAST
                     EAIAKCLPAVAGLLLADEIETITKVMEQPQRPLVAVIGGAKISDKIEVLNRLIDVADC
                     IAVTGAMANNFLLAQGVKVGKSLVESDVTGTAQEILHRARETEKNRPFSFLIPADVVV
                     STKMDGTAATRIVDLSSHSIADIESYPKLPRPTAYTVEPDEMILDIGPLSALHIAGVI
                     KFCKTVIWNGPCGVTEVKGMAGAQNPFAHGTKIIADAMIGESNQDKNKPYSLVGGGDT
                     AAYVESQGLVEDFNFVSTGGGASLELMSGHKLPGVEVLQDK"
     gene            2131..2910
                     /gene="tpiA"
                     /locus_tag="VEH48_00435"
     CDS             2131..2910
                     /gene="tpiA"
                     /locus_tag="VEH48_00435"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXY17873.1"
                     /translation="MRKILIVGNWKMHLNASQASLLVHRLHERIMIHRDIEVVLAPSM
                     LTLQPMSVQIDRRKFRLAAQNAYFHDEGAFTGEVSFTMLQELVHYAIVGHSERRRVFH
                     EDLDMVRDKVAAAVRNNITPILCVGETKHERQEGETKQVLHDQLLTGLSNLTAGEVGA
                     MVVSYEPVWAIGTGDVAKPDQVETATTYIRGYVNDVFGPKAAKNLRILYGGSVVPDVV
                     GGFLNIPGVDGFLVGGASLNYHEFSSIVNAARRWQRDKGGE"
     gene            2914..3633
                     /locus_tag="VEH48_00440"
     CDS             2914..3633
                     /locus_tag="VEH48_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17874.1"
                     /translation="MPRDDEDRVFDVSKPHKITPSPTSKPVIVGHHPQAADPMVNQRP
                     SEPRHGTDGASSIRVPISVSESEPTGLGGEKPGVPLMEHHAPESKEPFVVPAVEPVPE
                     PKPAESVPAEPALETKTAGSPPPEPAAVYPGAEKDQSAAAEVAPTATHVTAPVPVVPA
                     PDAGPSLDDLPLPSHHGPHNGHSHAAHTPAQTKHGRDNGKLLWVIAILVLLAGVYAIL
                     DATTSSMPFHIFKKSTSSLTQ"
     gene            3696..5819
                     /locus_tag="VEH48_00445"
     CDS             3696..5819
                     /locus_tag="VEH48_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXY17875.1"
                     /translation="MHKELLAGLNPEQKRAVETTDGPLLILAGAGSGKTKTLTHRIAY
                     LLASGKATPFNILAVTFTNKAAGEMRRRIAKLAGQDPDNRGFMPYMGTFHSICVRLLR
                     RDGEAIGIPRNFVIWDELDRLAAVKQAAKQLSIDEKAFPGRLLASLISGAKNEMIGPT
                     EYAALASSPAQHAAAKVFPAYEKALKEAAALDFDDLISRAVQLLKSHNAVRERWQKQF
                     AYVMIDEYQDTNAAQYQLVKLLTGPARNIAVVGDDWQSIYSWRGADFRNILNFEKDYP
                     KCTVIKLEQNYRSSKNILDAAHSVISHNRQRSDKKLWTAAGAGAPVQILSVNSERAEA
                     EAIVRRIQMAADIKARNYRDFAVLYRTNAQSRALEEVFVHHGLPYRIVGGVRFYDRKE
                     IKDILAYLRLIYQPEDSASFERIVNVPVRGIGTKSLDVFHAWRLANNLGLLEGLKKVG
                     SCGKLTAKARLGFSQLGDIINSFRAQMDELAPSALIDGLLRRLDYLHLLEDGTPQGEA
                     RVENVRELLSVAAEYNDMGLDGFLEEVSLVSDLDNTDFGSNFVTLMTLHSAKGLEFPV
                     VFITGLEETVFPHSRALYDANELEEERRLMYVGMTRAREELYLAYATSRTLYGGRLSN
                     PPSRFLADVDGLAAAPVDLAPAGKITDEPRYVPELAEGDGVKHRTFGKGTVLELDGDI
                     ATIYFKNLGTKKLDVGFAPMVKLEE"
     gene            5816..7099
                     /locus_tag="VEH48_00450"
     CDS             5816..7099
                     /locus_tag="VEH48_00450"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HXY17876.1"
                     /translation="MNKIIMPKKARTTADYYQSVQDKGGSFLRRRPYLIPVFGLLIGA
                     VMVGGVLLAHGGRPARPSDAHVVFLFDEGKQETLDTKAATVGQLINKLPLNLIPQDVV
                     EPSQDTPIVEDNFRINVYRARPVTVVEGNSKTVTLTAQKSARQVAEQAGLHIIPEDIA
                     TFAQGNLKDNIIGEQVIVSPATPINLNLYGAQFPAYTQAKTVGGLLAEKHIKLENGEN
                     VSPAVTTEITPGMQIFVLNRGVQVATMEETIPAPVQDIADSSLSFGTTVVRQTGVPGK
                     ELDTYLISNGSGTPAGRKLIQQVIIQSPVPQIVAVGTVIDIDSAKIKLMEEAGISSSD
                     LQYVNYILSRESGWCATKLQGQVGYCPPSPPDSIPSYLGYGLGQATPGSKMASAGADW
                     ETNPVTQLIWCNGYAVTRYGSWAGAYEHWAAYHNW"
BASE COUNT         1699 a         2026 c         2100 g         1406 t
ORIGIN      
        1 aacattgacc gtacccactt tgacatcacg gcccacgcca aggtccagaa cgccactgtt
       61 accaactatg ttttcacggt tgttaacagc cagggccagg ctgtggacac cgctactgtc
      121 actaccagtg ctttaagcgc ggtctaccac tttaaccaaa gcgccgccgg cacctatacc
      181 gtaaacgtgg tcatcaacac cgaccaaggc agtactaagg gtaagtgtga ttgcagtgtt
      241 aaggtgaccg tcaaaccggc gcctacgccc ccggttacac cgcccgttac tccgacccct
      301 ccgactaccc ctccgactac ccctaaagtc caggccgcta ccactttacc gaataccggt
      361 cctggtgaca taggtgctat gttcgttggt acgtccgctt tcggtggcgt cggccactac
      421 ctggtcagcc gccgcaagcg ataagcgtgt ttgttcataa aaacctgtgg ataacaggtt
      481 gagaaaaatg tcagaatgtg ctagggtaaa tgttaggaac cagagcattg actgaccgct
      541 ccaatccaaa acaaaaattt gcaagcatcc aataaatccc ctgttggcta tggtggagtt
      601 agcccagggg atttttggtt ggattgaaaa acaatggtga ccccaagggg aatcgaaccc
      661 ctgttgccgg gatgaaaacc cggtgtccta gccgttagac gatggggcct tcattatctt
      721 tcaatcaccg ttattctagc atttgccgga taagtttgac atactgttta atagatacgg
      781 aaagggtggc aattgtttaa caaaaagacc ataaaagaca tagaactgaa cggccagcgc
      841 gtgctgctgc gcgccgatta taacgtgccg gtggaaaatg gccggataac cgatgattac
      901 cggatgaaac gctcgctgcc gaccctccgc ttcatcttag accagcccgg aaccagcata
      961 gtgattattt cgcatctggg gcggcccaag gggccagaag ataaagacct aagcctcaaa
     1021 ccggtagccg agcacctgag caagttgctg gacaaagaag tgggatttgt aggcgattgt
     1081 atcggtgaag aggccaaaag ggcggccgac agcttacaag ccggcggcat cttgatgttt
     1141 gagaatgtcc gctttcaccc ggaagaggag aagaatgacc cggctttcgc taagcagctg
     1201 gccgatgccg ccggcgccca gttgttcgtc caggacggct ttggcgtggt gcaccgggcg
     1261 cacgccagca ccgaggccat cgccaagtgc ttgccggcgg tggccggctt gctgctggct
     1321 gacgaaatag aaactattac caaagtcatg gaacagccgc agcgcccgct ggtggcggta
     1381 atcggcggtg ctaagatcag cgacaaaatt gaggttctga accgtttgat agatgtggct
     1441 gactgtattg ccgtgaccgg cgccatggcc aataactttc tactggccca aggagtgaaa
     1501 gtcggcaaaa gcttggtgga atcggacgtt actggtactg ctcaggagat tttgcaccgg
     1561 gcccgcgaga cagagaaaaa tcgcccgttc agctttttaa taccggcaga tgtggtggta
     1621 tctactaaaa tggacggcac ggcggccacg cggatagtgg atttatccag tcacagcatc
     1681 gccgacattg aatcttatcc caaattgccc aggccgacgg cttacaccgt ggagcccgac
     1741 gagatgatcc tggacatcgg tcctttgagc gctctgcata tcgccggtgt gattaaattc
     1801 tgcaaaacgg tgatttggaa cggcccgtgc ggcgtgaccg aggtaaaagg catggccgga
     1861 gcccagaatc ctttcgccca cggcactaag atcatcgccg acgctatgat cggcgagagc
     1921 aaccaggata agaacaagcc ctacagtttg gtgggcggtg gcgacaccgc ggcctacgtg
     1981 gaatctcagg gcctggtgga agacttcaat tttgtatcta ccggcggcgg cgccagccta
     2041 gagctgatga gcgggcacaa gttgccgggt gtggaggttt tgcaagataa atagatggta
     2101 caatagcgta aggattataa gggtggctag atgcgcaaga ttctaatagt cggtaattgg
     2161 aagatgcacc tgaacgcgtc gcaggccagc ctgctggtgc accggctaca tgagcgcatc
     2221 atgatccacc gcgacataga ggtggtgctg gcgcctagca tgctgactct gcagccgatg
     2281 agcgtgcaga tagaccggcg taaattccgg ctggccgctc aaaacgctta ttttcatgac
     2341 gagggggctt ttaccggcga agtcagcttt accatgctgc aggaactggt ccattacgcg
     2401 attgtcggcc acagtgagcg gcggcgggtt ttccacgagg acctggatat ggtccgcgac
     2461 aaggtcgccg cggccgtgcg taacaacatc acaccgattc tatgcgttgg cgagaccaag
     2521 cacgagcgcc aagaaggtga gaccaaacag gtactgcatg accaattgct gaccggtctg
     2581 tccaacctga cggccggtga agtcggggcg atggtggttt cttacgaacc ggtgtgggct
     2641 ataggtaccg gcgatgtggc caagcccgat caggtggaga cagccactac gtatattcgc
     2701 ggctacgtca acgacgtctt tggccccaaa gcggctaaga atctgcggat cttatacggc
     2761 ggcagcgtag tgccggatgt ggttggcggc tttttgaaca taccgggtgt agacggtttt
     2821 ttggtcggcg gcgccagcct gaattatcac gaattttcgt caatcgtgaa cgcagcccgg
     2881 cgctggcagc gggacaaggg aggcgaataa ccaatgccgc gcgacgatga agacagggta
     2941 tttgacgtta gcaaaccgca caaaatcacg cccagtccga ccagcaagcc ggtaattgtc
     3001 gggcaccacc cgcaggcggc agacccgatg gtcaaccagc ggcccagcga gccgcggcac
     3061 ggcaccgatg gcgccagcag catacgcgta cctatttcag tgagcgaaag cgaaccaacc
     3121 ggcttgggcg gtgagaaacc gggtgtgccg ctgatggagc atcacgcgcc cgagagcaaa
     3181 gaaccatttg ttgtgccggc cgtcgagccg gtaccagaac caaagcctgc cgagagcgtg
     3241 ccggctgagc cggcgctaga aacaaaaacc gcaggaagtc cgccccctga accggcggcg
     3301 gtttatcccg gagccgagaa agatcaatcc gcggcagcgg aagtagcgcc gacagccacc
     3361 cacgtgaccg caccggtgcc ggttgtgccg gcgcctgacg ccgggccgtc gctggatgat
     3421 ttacctctgc ccagccacca cgggccgcac aacggccaca gccacgcggc ccatacgccg
     3481 gcccagacca agcacggccg cgataatggc aaactgttat gggtgatagc gattctggtt
     3541 ttgttggcgg gggtttacgc gattttagac gccacaacct caagcatgcc cttccatatc
     3601 tttaaaaaat ccacatcctc gctcacccaa taaccctgga tactaccgag gggaacctgg
     3661 cagtaatcac tcttcgcacc tgttaaaata ggtgggtgca caaagagttg ttggcggggt
     3721 tgaaccccga gcaaaaacgg gcagtggaga ccaccgacgg cccgctgctg attttggctg
     3781 gcgccggcag cggcaaaacc aagactttga cccaccgtat cgcttatttg ttagccagcg
     3841 gcaaggccac accgttcaat atcttggctg tgactttcac taataaggcc gccggcgaga
     3901 tgcgccggcg gatagccaaa ctggccggcc aggacccgga caaccgcggt tttatgccct
     3961 acatggggac ttttcacagt atctgcgtac gtctgctgcg ccgcgacggc gaggccatcg
     4021 gtatcccgcg caactttgtt atctgggacg aattggaccg gctggcggcc gttaaacaag
     4081 cggcgaaaca gcttagtatt gatgaaaaag cttttcccgg gcggctgctg gccagcctga
     4141 tcagcggcgc caagaacgag atgatcggcc ccacggaata cgcggcgctg gccagcagcc
     4201 cggcgcagca cgccgcggcc aaggtgtttc cagcctacga aaaagccctg aaagaagccg
     4261 cggccctgga ctttgatgat ctcatcagcc gcgctgtcca actgcttaaa tcacacaatg
     4321 ccgtgcgtga gcgctggcag aaacagttcg cctacgtgat gattgacgag taccaagaca
     4381 ccaacgccgc ccagtatcag ttagtcaagc tgctgaccgg cccggcgcgg aatatcgctg
     4441 tggtgggcga cgactggcaa agcatttaca gctggcgcgg cgccgatttc cgcaacatct
     4501 tgaattttga gaaagattac cccaagtgca cagttatcaa gctggagcaa aactaccgca
     4561 gcagcaaaaa cattctggat gcggcgcaca gcgtcatcag tcacaatcgt cagcgcagcg
     4621 ataaaaaact ttggacagcc gccggcgccg gcgcgccggt acaaatccta tctgttaatt
     4681 ccgagcgcgc tgaggccgag gccatagtgc gccgtatcca gatggcggcc gacatcaaag
     4741 cccgtaatta ccgggatttc gccgtgctct accgcaccaa cgcccaaagc cgggcgctgg
     4801 aagaagtctt tgtacaccac ggcctgccgt accgcatcgt cggcggcgtc cgcttttacg
     4861 accgcaagga aatcaaagat atcctggctt atctaaggct gatttatcag ccggaggaca
     4921 gcgccagctt tgagcgcatc gttaacgtgc cagtacgcgg catcggcacg aaatctttag
     4981 atgtttttca cgcctggcgc ctggccaata atcttggcct gctggaaggc ctgaaaaagg
     5041 tcggcagctg tggcaagctg accgctaaag ccaggctggg ctttagccaa ctgggcgata
     5101 tcatcaatag ttttcgggcc cagatggacg agctggcgcc ttcagcgctg attgacggct
     5161 tgctgcgccg gctggattat ctgcacctgc tggaggacgg cacaccgcag ggcgaggccc
     5221 gggtggagaa cgtccgtgaa ctgctgagcg tggccgctga gtataacgat atgggtctgg
     5281 acggcttttt ggaggaagtg tcgctggtat ctgatctgga caacaccgat tttggcagta
     5341 atttcgtcac gctgatgaca ctgcattcgg ccaaaggcct ggagtttccg gtagtcttca
     5401 taaccggtct ggaagaaacg gtctttccgc acagccgggc actatacgac gccaacgagc
     5461 tggaggagga acgccgcttg atgtacgtcg gtatgacccg cgcccgcgaa gagctctatc
     5521 tggcctatgc cactagccgg acgctttacg gcggccggct gtccaatccg cccagccgtt
     5581 ttctggcgga cgtagacggc ctggccgccg cgccggtgga cctggcgcca gccggcaaaa
     5641 tcaccgacga gccgcgctat gtaccggagc tggccgaggg cgacggggtc aaacaccgga
     5701 ctttcggcaa gggaacggta cttgagctgg acggcgacat cgcgaccatc tattttaaga
     5761 acctcggcac aaaaaaactg gacgtaggtt tcgcgccgat ggtcaaacta gaggaatgaa
     5821 taagataata atgcccaaaa aggcccggac aaccgcggat tactaccagt ccgtacagga
     5881 caaaggcggt tcgtttctac gccggcggcc ttatctgata ccggttttcg gcctgcttat
     5941 cggcgcggtg atggtcggtg gtgtgctgct ggcccacggc ggccggccgg cgcggcccag
     6001 tgacgcccac gtggtttttc tgtttgacga gggtaagcag gaaactctgg acaccaaagc
     6061 cgccaccgtg ggccagctaa tcaataaatt gccgctgaac ctgataccgc aagatgtcgt
     6121 cgaaccgtcg caggatacgc cgatagttga ggataacttc cgcatcaacg tctaccgcgc
     6181 ccgaccggta acggtggtgg aaggcaacag caaaaccgtc accctgaccg cccaaaaaag
     6241 cgcccggcag gtcgccgagc aggccgggct gcatatcata ccggaagaca tcgccacttt
     6301 cgcccaaggt aatcttaagg ataatatcat cggcgagcag gtgattgtct cgccagccac
     6361 gcccatcaac ctcaaccttt acggcgccca atttccggcc tacacccagg ccaaaaccgt
     6421 cggcggtctg ctggcggaaa agcacatcaa gctggaaaac ggcgagaacg tatcgccggc
     6481 tgtcaccact gaaatcacgc ccggtatgca gatttttgtg ctcaaccgcg gcgtacaggt
     6541 ggcgaccatg gaagagacca tcccggcgcc ggtgcaggac atcgccgatt ccagcctgag
     6601 tttcggcacc accgtcgtac gccagaccgg cgtgccgggc aaagaactgg acacctactt
     6661 gatcagcaac ggcagcggca cgccggccgg ccgcaaactt atccagcagg taattatcca
     6721 gagcccggta ccacagatcg tagctgtggg tacggttatt gatattgata gcgccaaaat
     6781 caagctgatg gaagaggcgg gcatctccag ctccgacctg cagtatgtga actacatctt
     6841 aagccgcgag tctggctggt gcgccaccaa attgcagggc caggtcggct attgcccgcc
     6901 gagcccgccg gatagcattc ccagctatct cggctacggg ctgggacaag ccacgccggg
     6961 gtccaagatg gcctctgctg gcgccgactg ggaaaccaac ccggtgaccc agctcatatg
     7021 gtgtaacggt tacgctgtga ctcgttatgg aagctgggca ggggcctatg agcattgggc
     7081 ggcgtatcat aattggtgat tttagcaccg gtttctcttt tggagtaaaa taaaagtgtg
     7141 gaaattgcaa aaaaatcgct ggggcagcat tggctcgcgg acgccgaggc tctagaggcg
     7201 attactgaat acgccgagat tgagtccagc g
//
LOCUS       DASPTF010000016         3101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_740548, whole genome shotgun sequence.
ACCESSION   DASPTF010000016 DASPTF010000000
VERSION     DASPTF010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740548"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2172)
                     /locus_tag="VEH48_00455"
     CDS             complement(<1..2172)
                     /locus_tag="VEH48_00455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXY17877.1"
                     /translation="MWCRQKILQMSGVLTVGLAVLISIFALSVQADSSASWLGDAPLR
                     LQQESNLSSVPGSLNGNIDCQQEDGANCVVATLYGSASSSGSIRLNGTGNYYPVFTQI
                     DGQRHFLAVPNSDIGISYSITPQNGLYLYFNHGLSASLKSVEVNVPGGFSWEYHINSP
                     PDGRLQDQGGNLLAADYDSMSFSADGHWMVVTVPNITTVRVNLQTLEVLPFGPGFNYG
                     IGLAPAAQTAISNDGRYAVVASKNFGIFQIYDLSTCAAPPPHITAPVDCQLRDLQSYM
                     QGKITGFINASNIRFISDDVLGVYVSSGQLTEPTVTKYIMNTGANSWHQLDYLTLGDS
                     YISGEGAYDYLAGTDTSDNKCHVSAISYPYLLGRDLDYNSYHSVACSGAITRDILNRR
                     DDYPGQVQLKIARNKRNVSNILGSFLPGYIDQLDFVSQYQPKVVTLSIGGNDMGFSSI
                     LLACVEPGTCYNSYEDRLELVQQINNSVFPRLINTYQQIKNAGAPDARIYVIGYPQIA
                     EPGGDCADNVHLDTDEVEFSAQIINYLDNVIQAAAARAGVTYVDTQHALDGHRLCEAA
                     SKYEAAVNGLTAGNDRPRSISGPIADESYHPNFIGHELLEQAILQATNGLSEPMPPAD
                     PLAGLPAINGLDILNVPKNGRNVNTAEYDPTISDDVIYKDAADDLIINGADHALSPGT
                     NLQAEIHSDAVALGNFTTSPDGNLFAKVSVPDDIADGYHILH"
     gene            complement(2395..2486)
                     /locus_tag="VEH48_00460"
     tRNA            complement(2395..2486)
                     /locus_tag="VEH48_00460"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2448..2450),aa:Ser,seq:cga)
     gene            2573..>3101
                     /locus_tag="VEH48_00465"
     CDS             2573..>3101
                     /locus_tag="VEH48_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17878.1"
                     /translation="MQFVRKALLVLLAPLFTLLLYATAVDVGFVRVGTHPSTIKSILA
                     KSGIYDTVVPGLLNQGKQISSSGGSVSLSNSLVRTAAGQTFTPQLVQQNTEATIDGVY
                     DWLDGKTPLPDFQIDLTNTKTAFAANVAKAAQQQLQALPVCSSAASAENFDPLTASCL
                     PPGVTPASAAATLQSN"
BASE COUNT          743 a          782 c          819 g          757 t
ORIGIN      
        1 atgcagtatg tggtaaccgt ccgctatgtc gtcaggtaca ctaactttgg caaagagatt
       61 accatccgga ctcgtagtaa aattgcccag cgcaaccgca tcggaatgga tctcggcttg
      121 caaattagta cctggagata acgcatgatc agcaccatta atgattaaat cgtccgcggc
      181 gtccttataa atcacgtcat cggatatggt tgggtcgtat tccgcagtgt ttacgttccg
      241 gccgttcttg ggcacattca aaatatccaa gccattgatg gctggcagtc cagccaacgg
      301 gtccgctggc ggcattggtt cgctcagacc attggtggct tgcagaatgg cctgctccag
      361 cagttcgtgt ccgatgaagt tggggtggta gctttcatcg gcgattgggc cactaatact
      421 ccttggccgg tcgttgccgg cggtcaagcc gttaaccgca gcttcatatt tgctggcggc
      481 ctcacacagc cggtgaccat ccagtgcatg ttgcgtgtct acgtaagtta ccccggctct
      541 ggcagccgcg gcttgtataa cgttatccaa ataattgatt atctgggcag aaaactccac
      601 ttcatcggta tccaaatgaa cattgtcggc gcaatcgccg ccgggctcgg ctatttgcgg
      661 gtagccaatc acatagattc tggcatccgg cgcgccggca ttcttaattt gctggtaggt
      721 attgatcagc ctcgggaaaa ccgagttgtt gatttgctgg accagctcca aacggtcctc
      781 atagctgttg tagcaggttc cgggttcaac gcaggccagc aagattgaag aaaaacccat
      841 gtcgttgccg ccaatcgaaa gtgtcacgac cttaggctga tattggctga caaaatccag
      901 ctggtcaata tagccgggca gaaaagaacc taaaatgttt gagacgttcc ttttgttgcg
      961 tgcaatttta agctgaactt gcccaggata atcatccctt ctatttaaaa tgtctcttgt
     1021 tattgctcca gagcatgcga ccgagtgata agaattgtaa tccaaatctc gccccagtaa
     1081 atacgggtaa gatattgcgg acacgtggca tttattgtca ctggtgtctg tccccgccag
     1141 gtagtcgtag gcgccttcgc cggaaatata tgaatcgcca agcgtcaagt aatccagctg
     1201 gtgccagctg ttggcgccag tgttcattat gtatttggta accgtcggct cggtcagttg
     1261 gccggaactg acgtacactc ccaagacatc atcacttata aatctgatat tgctggcatt
     1321 aatgaaacca gtgatcttac cttgcatgta gctttgtaaa tctcgcaact ggcaatcaac
     1381 cggcgcggta atgtgcgggg gaggagcagc gcaagtgctt aaatcgtaaa tctgaaaaat
     1441 gccgaaattt ttggaggcca ctacagcgta gcgtccatca ttgctaatgg ctgtttgggc
     1501 ggccggcgcc agaccgatgc cgtagttaaa tcccgggcca aacggcagca cttccagagt
     1561 ctgtaaattc acccggactg tggtgatgtt cggtaccgtt accaccatcc agtggccgtc
     1621 agccgaaaag ctcatggagt catagtctgc cgccagcaaa ttgccacctt ggtcttgcag
     1681 ccgcccgtcc ggtggggagt taatgtgata ctcccaggaa aatccgccag gaacattgac
     1741 ctcaacagat ttaagcgacg cagatagccc gtggttaaaa tatagataaa gcccgttttg
     1801 cggggtgatg ctgtagctga taccgatgtc ggaattgggc acggctaaga agtgccgctg
     1861 accatcaatt tgcgtaaaaa ccgggtagta attgccggtg ccgtttaggc ggatagaacc
     1921 gctggagctg gcgctgccat agagggtagc gaccacgcag ttggcgccat cttcctgttg
     1981 gcagtcgatg ttaccgttga gagaaccggg cacggaagat aaatttgact cttgctgcag
     2041 ccgcaagggt gcatcgccta accaagaggc agaagaatcg gcctggaccg atagcgcaaa
     2101 aatactaatt aaaaccgcta gaccaacggt taaaacacca ctcatctgca ggattttttg
     2161 tctgcaccac ataaacataa acgcttattt gacaataagt caaattatat caacctgcgt
     2221 tattgatggc taatccggtt ttctgacgat ctagcgggaa atacttaaca agtcaagtaa
     2281 ctgggttccc cgttgcatgg ctctgatgca atggggtgga gcctaaggac gtgcgaaagc
     2341 tggaagtaaa atggccttaa gctgttttac ggaagctgta gtcacggcct aagctggcgg
     2401 aggaggcgag attcgaactc gcgacagagt tgcccctgta ccgcttttcg agagcggcgc
     2461 actagaccgg actatgcgac tcctccgtta actagtatac atctacctct gttactttta
     2521 gggggctggc cattttattg gttaaaaatc ttccttgccc tacaataggc ttatgcagtt
     2581 tgtgaggaaa gcgctgttag tgctgttggc accgttgttc acgttgttgc tttacgccac
     2641 ggcggttgac gtcggttttg tgcgggttgg tacgcacccg tcgaccatca aaagcattct
     2701 ggccaaaagc ggcatttacg acacagtggt gccgggcctg ctgaaccaag gcaaacaaat
     2761 ttccagcagc ggcggcagtg tatctttgag taattctctt gtccgtacgg cggccggaca
     2821 gacctttacg ccgcagctgg tacagcagaa caccgaagcg acgattgacg gcgtttacga
     2881 ctggctggac ggcaaaacgc ccttgccgga ttttcagatt gatttgacta acactaagac
     2941 ggcctttgcc gccaatgtgg ccaaggcggc gcagcagcag ctgcaggctt tgccggtttg
     3001 cagctctgcc gccagcgccg aaaattttga cccgctaacc gccagctgcc tgccgccggg
     3061 cgtgacgccg gccagcgccg ccgcgactct gcagagcaat a
//
LOCUS       DASPTF010000017         5584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1457699, whole genome shotgun sequence.
ACCESSION   DASPTF010000017 DASPTF010000000
VERSION     DASPTF010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457699"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VEH48_00470"
     CDS             complement(<1..540)
                     /locus_tag="VEH48_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17879.1"
                     /translation="MAKRKVIAFDLDGTLTASKSPVTDRMAELIDRLLDKYQVCVMSG
                     GKFEQFEEQFLAHLKSEPSKLARLHIMPTCGTRYYRYEKGKWKQKYAEDFTASQKTEI
                     IKALNAGIDHFGFREPKTWGDIIEDRGSQITFSALGQDIVAKLGEEGVRLKEAWDPDV
                     KKKKKLRDYVANLIPEFEVR"
     gene            complement(527..1060)
                     /locus_tag="VEH48_00475"
     CDS             complement(527..1060)
                     /locus_tag="VEH48_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HXY17880.1"
                     /translation="MKKPKELVNDTVDRDWGAPIVSFQEFAKLRPKLGKVVATSGGFD
                     PIHPGHISCIIDSKKYGDTLVVIVNGDAFLTAKKGRPFQNLETRCLIVSGIVGVDYVI
                     PFEIHGDQTVAKALEALKPDVFTKGGDRLAKAHLPEAEQAAIDKYKIKTVYGVGEDKK
                     WSSSWFLKEWDEGNGKT"
     gene            1175..1762
                     /locus_tag="VEH48_00480"
     CDS             1175..1762
                     /locus_tag="VEH48_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17881.1"
                     /translation="MARKSRTFKLTRKAGLISAAALVVVIASSLGAWHYHDRTNQETL
                     PVAPATNTTYNQTNSNNQSVGSSDKQAAPAGGYSATTTSNSPSNNTGQPPLTPSPPFV
                     SNHKPGLNGTPTTENSVCNTTPGASCYIEFTMGNKTIKLQAGTADTNGSVYWENWDVN
                     NPSYGFSSGSWTITAVATLNGQTQTATDSQNLVIQ"
     gene            1759..3792
                     /locus_tag="VEH48_00485"
     CDS             1759..3792
                     /locus_tag="VEH48_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17882.1"
                     /translation="MMKKILLSFTTMLALSVAAALIFHPAPAHADFNANNLMDDDIFT
                     AVNSMSAAGIDSWLNQFPNSCISTNNGFSAPDPTGYTPSNGFTYDGNVSAGQVIYDAA
                     QGYGINPQVLLVTLQKEQSLVTGSAGCSILQYAGATGYGCPDGGTTYSYNGVDLYSIN
                     GNEVTSVSGTCVNSSARAGFSQQVIHAAWLLMFGEQRSKGNISWDVQKTNSPEQGDVY
                     DDSDDPQTCYGGPMTQGTWVVCPGGSATFYDGSYTIDGTTVQITTGATAALYWYTPHF
                     AGNQNFDSLFQSWFGSFYVPQYSWQLLGFSYSTNTNSFGAGVAGTATLTAKNTGTVPW
                     YNYGANPVRLGTWLPGRVSPLYSPSWLSPTRPANMNQVEVDPGGTATFTIPIDITALG
                     TYVEGLNLVVENSQWMPWPGLSPTINIQPAYQWQVSSVTYSAGTGLMIPGTTQQITVT
                     AINSGFATWSNSSGPPIRLGTWLPGRVSGVAYNWLGSARAAGLTEASVAPGQTGTFQF
                     MVRTPGSGIFYERMNLVAEGQTWFNDAGLTLYLQGGTYSWKPLWSTYSTGGNANLPRG
                     TTMTVTIKALNTGNIPWTNTTSASFPWPIRLATDDPENRGSFLYDTSWINDTRPATLQ
                     ESTVQPGQQGTFVFTADIPSSASTGQHFEYFNLVAEGILWFNDPGFYLYLNVL"
     gene            3849..4778
                     /locus_tag="VEH48_00490"
     CDS             3849..4778
                     /locus_tag="VEH48_00490"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HXY17883.1"
                     /translation="MDKTVLVTGGHGFVGTHLVKRLNELGAKVIAPTQAEYDLVDGIQ
                     VRAMLKANPCEMVIHLAAVVGGIGANREHPGKFFYDNLMMGTQLMHEAYKSKIPKFVA
                     IGTICAYPKFTPIPFKEEDLWNGYPEETNAPYGLAKKMMLVQSQGYRQEYGFNSIFLL
                     PVNQYGPGDNFDPSSSHVIPALIRKCVEAQESNAEFIEAWGDGSPTREFLYVKDTVEA
                     IVLAAEKYNKSDPVNLGSSFEISIKDLVELIAKETGFEGEIKWDTSKPNGQPRRKLDT
                     SRAEKEFDFKSETTFQDGLRETIKWYKENKTSA"
     gene            4771..>5584
                     /locus_tag="VEH48_00495"
     CDS             4771..>5584
                     /locus_tag="VEH48_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXY17884.1"
                     /translation="MRSLDDTIVIEAGKTSRISIKELWLYRELFYFFAWRDIKVRYKQ
                     TVLGAGWAILQPLLAAGIFTLFFNHVAKISSGASNVPYPVFAYLGLMYWNAFAAALGT
                     VSNSLLSNSGVLTKIYFPRLIPPLAATALSIVDFFFAAIVFFIIMLGYRVSPGWQGFA
                     LIIPSLVLLTVIALGVGLFFAALNVKYRDVRSALPFIIQIWFFVSPVIYPVSYIPARF
                     QSLVYLNPVTGAISSVKQGLFHEANDWHGLLISWVVALIMLAIGLWYFKRTEK"
BASE COUNT         1396 a         1404 c         1312 g         1472 t
ORIGIN      
        1 tcttacttca aactccggga taagatttgc gacataatcg cgtagttttt tctttttctt
       61 aacgtctgga tcccacgctt ccttaagccg cacgccttcc tcgcccagct tggctacgat
      121 gtcctgcccc aacgccgaaa aagttatctg gctgccgcgg tcttctatga tatctcccca
      181 ggtttttggc tcgcgaaaac caaagtggtc gatgcctgcg ttcagggctt tgatgatttc
      241 ggtcttttga gaagcggtga aatcttcggc gtatttttgc ttccatttcc ccttttcata
      301 gcgataatag cgcgtgccgc aagtcggcat gatatgcagt ctggccagtt tggacggttc
      361 ggatttaaga tgggccagga actgctcctc aaactgttca aacttaccgc cgctcatcac
      421 acagacctgg tacttatcca ataaacggtc tatgagttcc gccatgcggt cggttaccgg
      481 cgacttgctg gcagtcagcg tgccgtccaa gtcaaaagct atgactttac gttttgccat
      541 tcccttcgtc ccattcttta agaaaccagc tgctggacca ttttttgtct tcgccgacac
      601 cgtaaacggt tttgatttta tatttatcaa tcgcagcctg ctcggcctcc ggcaagtggg
      661 ccttggctag ccggtcgcca cctttggtaa aaacatccgg cttcaaggct tccagggctt
      721 tggcgaccgt ttggtcgcca tggatctcaa atgggataac gtaatctacg ccaacgatac
      781 cgctgactat caagcagcgc gtttccaagt tttggaacgg ccggcctttt ttggcggtca
      841 aaaaggcgtc gccgttgaca atcaccacca gtgtatcacc gtactttttg ctatcgatga
      901 tacagctgat atggccgggg tgaatcggat caaaaccgcc gctggtggca actactttgc
      961 ccaattttgg ccgcagtttg gcaaattctt ggaacgaaac aatcggcgca ccccagtctc
     1021 tgtctactgt gtcgttgacc agctctttcg gttttttcat attgctacca aaacagtata
     1081 gcaggcgcaa atctacagat tgacataaga gataagaaag tcttagaata aatgcacaac
     1141 aaacacgtgc cacttggcac ggtaaaataa agacatggcc agaaaatcca gaacattcaa
     1201 gcttactaga aaagcgggcc tgatatccgc ggcggcgctg gtcgtagtta tagcctccag
     1261 tctcggcgct tggcattatc atgaccgtac taatcaagaa actctcccgg tggctccagc
     1321 tacaaatacc acttacaatc agaccaattc taataatcaa tccgttggtt cgtcagataa
     1381 gcaggctgcg ccagctggtg gttatagcgc aacaaccact tcaaatagtc ccagcaataa
     1441 cactggacag ccgccgctta ctccaagccc tccctttgtt agcaaccata aacctggact
     1501 aaatggcacg ccgactactg agaactcggt atgtaacaca acacccggag ccagctgtta
     1561 tatagaattt acaatgggta acaaaactat aaagctacaa gctggcacag cggatactaa
     1621 tggctccgta tactgggaga actgggatgt taataatcca tcttatggat tcagctcggg
     1681 tagttggact atcacagccg tagcaacact aaatgggcag acacaaacag caacggatag
     1741 tcagaatctg gtgatccaat gatgaaaaaa atcttgcttt cgtttactac aatgctcgct
     1801 ctttcagtcg ctgctgctct catcttccat cctgctccag cccatgcgga ctttaatgca
     1861 aataacctga tggatgatga catctttaca gctgtaaaca gtatgagcgc agcaggcatc
     1921 gattcgtggt taaaccagtt cccgaatagc tgtatcagca ccaacaacgg tttctctgcg
     1981 cccgatccga ccggctacac tccgtcaaat ggctttactt acgatggcaa tgtatctgct
     2041 ggccaagtca tttatgatgc cgcacagggc tacggcatta acccgcaggt gctgttagta
     2101 actcttcaaa aggagcaaag cctagtaaca ggcagcgcag gctgcagtat tttgcagtac
     2161 gcaggcgcta ccggttatgg ctgcccggat ggcggtacga cttatagcta taacggagtc
     2221 gatttataca gtatcaacgg caacgaggtg acgtcagtca gcggcacctg tgtgaacagc
     2281 agcgccagag ctggcttctc tcaacaggtg attcatgcgg cctggctact aatgttcggc
     2341 gagcagcgct caaaagggaa tatcagctgg gatgttcaaa aaactaattc accagaacag
     2401 ggagatgtgt atgacgattc agatgatcca caaacctgtt atggcggccc gatgactcag
     2461 ggcacctggg tagtttgtcc cggaggcagc gctacgtttt acgacggctc atacactatc
     2521 gatggaacta ctgtccagat aaccaccggc gccaccgcgg cactttattg gtacactccc
     2581 cactttgccg gcaaccaaaa ttttgacagt cttttccagt cctggttcgg ctcattttat
     2641 gttcctcagt atagttggca attgctcgga ttttcttaca gtactaatac taattcattc
     2701 ggagcgggtg ttgcaggtac ggcgactctc acagccaaga atactggcac ggtcccttgg
     2761 tacaactatg gcgccaatcc agttcggctt ggaacctggc tgcctggcag ggtaagccct
     2821 ctgtattcgc ctagctggct gtccccgact agaccggcta acatgaatca ggtcgaggtt
     2881 gaccccgggg ggacagctac gtttactatc cctattgaca ttactgcctt gggaacttat
     2941 gtagagggac tgaatctagt ggttgaaaac agccagtgga tgccctggcc cggtcttagc
     3001 ccaaccatca acatccagcc ggcataccag tggcaggtta gttccgtaac ctatagtgcc
     3061 ggtactgggc ttatgattcc cggaaccact cagcaaatta ccgttacggc gataaattct
     3121 ggttttgcca catggtctaa tagcagtggg ccgccaatac gtctaggcac ctggctgcct
     3181 ggcagggtaa gtggtgtagc ttataattgg ctaggttctg ccagagccgc cggtttgact
     3241 gaagcgagtg tcgctccagg tcaaactggc acattccaat tcatggtacg tacgcctggc
     3301 tctggtattt tctatgagcg catgaattta gtggccgagg gccaaacctg gtttaatgat
     3361 gccggtttaa cattgtatct acaaggtgga acttattcat ggaagccact atggtcgacc
     3421 tacagtactg gcggcaatgc taatctgccg cgtggcacca ccatgactgt gaccatcaaa
     3481 gcattaaaca ccgggaatat accttggact aatactacct ctgccagttt cccgtggcca
     3541 attcgcctag ctactgacga tccagaaaac cggggcagtt ttttgtacga tacaagttgg
     3601 attaacgaca ccaggccggc aactttgcag gaaagtacag ttcagcctgg tcaacaaggc
     3661 acattcgttt tcacagccga tattcccagt agcgcgtcta ctggacagca ctttgagtac
     3721 ttcaatttgg tcgccgaggg catcctttgg ttcaacgacc caggtttcta tctgtatctg
     3781 aacgtgctat agccatagac cttcgtaaca gatgaaacat gctatactct aaggagtaaa
     3841 tgttttggat ggataaaaca gtcttagtca ccggtgggca tggcttcgtt ggcacgcact
     3901 tggtaaagcg gctcaacgaa ttaggggcga aggtcattgc gccaactcaa gcggagtatg
     3961 atttggtgga tggaatccaa gtacgagcca tgctcaaggc taatccttgc gaaatggtca
     4021 tacatctggc agccgtcgtc ggcggtattg gcgccaaccg tgaacaccct gggaagttct
     4081 tttacgataa tctgatgatg ggaacccagc tgatgcatga ggcctacaaa agtaaaatcc
     4141 ctaaatttgt agctatcggc accatatgcg cttaccccaa atttacgcca atcccattta
     4201 aggaagagga tttgtggaat ggttatccgg aggaaactaa cgcgccctac ggcctagcca
     4261 aaaaaatgat gctggtccaa agccaaggct accgccaaga atacggcttt aactctattt
     4321 ttttgctgcc ggtaaaccag tatggcccgg gtgataattt tgacccgtcc agctcgcacg
     4381 ttatccctgc cctgattcgc aagtgtgttg aggctcaaga atcaaatgct gagtttatcg
     4441 aagcatgggg cgacggttcg ccaacccgcg aatttttgta tgtcaaggat acagtcgaag
     4501 ccattgtgct ggccgccgaa aaatacaaca aaagtgaccc ggtaaatctt ggctcgtctt
     4561 ttgaaatttc catcaaagat cttgtggagt taattgccaa agaaaccggc tttgagggcg
     4621 agatcaaatg ggatacctcc aagcccaacg gccagccgcg ccgcaaactg gacacttcgc
     4681 gcgccgagaa agaatttgat ttcaaatccg agacaacttt tcaggatggc ttgcgtgaaa
     4741 ctataaaatg gtataaagag aataaaacaa gtgcgtagtc tagacgatac aatagtcatt
     4801 gaggctggta agactagcag gatcagtatc aaagagctct ggctttaccg cgagcttttt
     4861 tacttttttg cttggcgcga catcaaggtc cgctacaagc aaacagttct gggcgccggc
     4921 tgggcgattt tacagccttt actggctgct ggaattttta cgttgttttt caaccacgtg
     4981 gctaaaataa gctccggcgc atctaatgtc ccttaccctg ttttcgcata cctgggcctg
     5041 atgtactgga acgcttttgc cgccgcgcta ggtacggtca gtaattcact tttatccaac
     5101 tccggcgtat tgactaaaat ttacttcccg cggcttatcc ctccgctagc agccacggca
     5161 cttagcatcg tggatttctt ttttgcagca attgtatttt tcattattat gctaggctat
     5221 cgcgtatcgc ctggatggca aggattcgct ttgataattc cgtccttggt attattaacg
     5281 gttatagctt taggcgttgg ccttttcttt gccgccttaa acgtaaagta ccgcgatgtc
     5341 cgttcagcgt tgcctttcat tatccaaatt tggttctttg tttcaccagt aatttaccct
     5401 gtttcttaca ttcccgcgcg ttttcagtca cttgtctatt taaacccggt aaccggcgct
     5461 atcagctccg ttaagcaagg actttttcat gaagccaatg attggcatgg tcttcttatc
     5521 tcatgggttg tagccttaat aatgctggcg ataggcttat ggtactttaa gcgaacagaa
     5581 aaga
//
LOCUS       DASPTF010000018         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1172228, whole genome shotgun sequence.
ACCESSION   DASPTF010000018 DASPTF010000000
VERSION     DASPTF010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172228"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VEH48_00500"
     CDS             complement(<1..1210)
                     /locus_tag="VEH48_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXY17885.1"
                     /translation="MNQLNLRSLRARKARLAASLRPYVYQVSLSLVLVGMAVAAWGGW
                     SGLNRLPFAAAALALLGLMLVFWYRLDLSQLPPRTPAASLDDILEDTLLGALRKNAPL
                     TPASAWQAACKLWPGRFLINHLLINEAEVGQLLDGAAEDMAVVWDSARQLAEKTSAAE
                     VDAGALAAALLTTSEAAKKYLAAHNLNAGEITETLDWQARLKRWLHQLRPYFGGIGRD
                     WAAGFTPELDHFGVNVSRAIESGRAGHYHTLAHADILDSVVHNLSQGSGALALVGEAG
                     AGKTSLAYALAQRLLEGRDESLLYYQLIELNAAQIISASGKELEKIAMTLFGEAAHAS
                     NVIIFLDDARLFFGQGTGAFDIGQVLMPLLQNRVIKIIAAFTPNEFQSLKAANEALAA
                     DFAIIAVKEPS"
     gene            complement(1207..1566)
                     /locus_tag="VEH48_00505"
     CDS             complement(1207..1566)
                     /locus_tag="VEH48_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17886.1"
                     /translation="MLFEFLGWWYGRGWLKAVQTARGWVREVEREFSAGILIKTLFSP
                     WKQIVSLPGQSIDEKFRATLDNLVSRSIGFIVRLITLLSAGVLLILALILGLLLVIAW
                     PFGPLAFVYLVFRSVKG"
     gene            complement(1632..2048)
                     /locus_tag="VEH48_00510"
     CDS             complement(1632..2048)
                     /locus_tag="VEH48_00510"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXY17887.1"
                     /translation="MSDYVILNADDAKDYYADSDVPGEFRRLTDKLGAEQLAITLIRI
                     PPHSDFEQGTGHYHDELEEIYIITKGTLTMRFGDDIKKVSAGSVVRVAPKTPRSHRNE
                     GDEPVDMWAISRQLGRRDATKIDDFWEASDQAKQHA"
     gene            complement(2066..>2265)
                     /locus_tag="VEH48_00515"
     CDS             complement(2066..>2265)
                     /locus_tag="VEH48_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17888.1"
                     /translation="TLTDFEDNTIKDTAEEDWTQDGDDYEPPKADTAEYAAGLIFKCI
                     QTGAAEIYAHEWMKDQDEGRA"
BASE COUNT          475 a          682 c          623 g          485 t
ORIGIN      
        1 ttgacggttc tttgacggca attatcgcga agtcagccgc cagagcttcg ttagcggcct
       61 tgaggctttg aaactcattg ggtgtaaagg cagcgatgat cttgatgacc cggttctgca
      121 gcagcggcat cagcacttgg ccgatatcaa aagcgccggt gccctggcca aaaaacaacc
      181 gggcatcatc cagaaagata atgacattgc tggcatgggc ggcctcaccg aacagcgtca
      241 tggcgatttt ttccagttcc ttgccggaag ccgaaataat ctgcgccgcg ttgagttcta
      301 tcaattggta ataaagaagg gattcgtcgc ggccttccag caatctttgc gccagcgcgt
      361 aggccaatga ggttttaccg gcaccggctt cgccgaccag cgccagcgcg ccactgccct
      421 ggctcaggtt gtgtacgacg ctatctaaga tgtcggcatg cgccagggtg tgatagtgcc
      481 cggcgcggcc ggattctatg gcccggctga cattgacgcc aaagtggtcc agttccggtg
      541 taaagccggc cgcccagtcg cgcccgatgc cgccaaagta cggccgcagc tggtgcagcc
      601 agcgtttgag ccgtgcctgc caatccaggg tctcagtaat ctccccggca ttcaaattgt
      661 gcgccgccag atattttttg gcggcctcgg aagtagtcag caaggccgcc gcaagggcgc
      721 cggcgtcaac ctcggccgcg ctggtttttt cggcgagctg ccgggcactg tcccagacca
      781 cggccatatc ttccgccgcg ccgtccagca gctgacctac ctcagcttca ttaattaata
      841 gatggtttat caagaaccgg cccggccaca gcttacaggc tgcctgccaa gcgctagcgg
      901 gcgttagcgg cgcgtttttc ctgagcgcgc ccagcagagt atcttccagg atgtcgtcca
      961 aactggccgc cggtgtgcgc ggaggtagtt ggcttaaatc cagccggtac caaaagacca
     1021 gcatcaggcc caatagcgcc agcgcggccg cggcgaaagg cagcctgttc aaaccggacc
     1081 agccgcccca cgcggcgacg gccatgccta ccagtaccag gctgagagat acttgataaa
     1141 cgtacggccg gaggctggcc gccaatcggg ccttgcgggc gcgcagactc cgcaggttaa
     1201 gctggttcat cccttcacac tcctgaacac cagataaaca aaggccagcg gtccgaacgg
     1261 ccaggcaatc accaacagca atccgaggat cagcgccagg atcagcagta ccccggcgct
     1321 gagcagtgtt atcagccgca cgataaagcc gatggagcgc gacaccagat tgtccagcgt
     1381 cgcccggaat ttttcatcta ttgattgccc gggcaggctg acaatctgtt tccagggaga
     1441 aaacaaggtt ttaatcagga tgccggccga gaactcccgc tccacttccc tgacccagcc
     1501 gcgggcggtc tgcacggcct tcagccagcc acggccatac caccatccca ggaactcaaa
     1561 aagcataaac atattgtatc ctaaaatcag ggatacggca gcagtttcaa tctatcggac
     1621 gtcaatccgt cttaggcgtg ctgtttggcc tggtctgaag cctcccagaa atcatcaatc
     1681 ttggtggcgt cgcggcggcc cagctggcgg gaaatcgccc acatatccac cggctcatca
     1741 ccctcgttac gatgcgagcg cggggtcttg ggtgccaccc gcactaccga accagctgaa
     1801 acttttttga tgtcgtcgcc aaagcgcatg gtcagcgtac ccttggtgat aatgtagatt
     1861 tcttcaagct cgtcgtggta atggccggtc ccttgctcaa agtccgaatg cggcggaatg
     1921 cgtatcaagg ttatagccag ctgttcggcg cccagcttgt ctgtcagccg cctgaactcg
     1981 cctggcacgt cgctatcagc gtaataatcc ttagcgtcat cggcgtttaa tatcacgtag
     2041 tcgctcatat agctatttta tcacctcagg cccggccttc gtcctggtct ttcatccatt
     2101 cgtgagcgta gatttcggcc gcgccggttt gaatgcattt gaatataagc ccggcggcgt
     2161 attcggcggt atcggctttg ggcggctcgt agtcatcacc gtcctgggtc cagtcctcct
     2221 ctgcggtatc cttgatggta ttatcttcaa aatcggtcag cgtta
//
LOCUS       DASPTF010000019        10064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_600889, whole genome shotgun sequence.
ACCESSION   DASPTF010000019 DASPTF010000000
VERSION     DASPTF010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10064
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600889"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VEH48_00520"
     CDS             complement(<1..183)
                     /locus_tag="VEH48_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HXY17889.1"
                     /translation="MAKIIGIDLGTTNSAMAAMQSGKPEIIANSEGGRTTPSVVAINK
                     NGERLVGQVANRQRVTN"
     gene            complement(202..609)
                     /locus_tag="VEH48_00525"
     CDS             complement(202..609)
                     /locus_tag="VEH48_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17890.1"
                     /translation="MATEETLRIHINPQNRSVSTTDPEYLEAWTAALEKRRAEIEAEL
                     RRQMENGELEIPVEEHSSGTVLKTGEAMQTTPEDALRFRAQSLAEGDMQNWAVMRGIG
                     MVGVTGGWDHGRTVEYVDDPEVPFLGYKFEEAA"
     gene            complement(609..1088)
                     /locus_tag="VEH48_00530"
     CDS             complement(609..1088)
                     /locus_tag="VEH48_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXY17891.1"
                     /translation="MSSKKPTIQDLQQQVAELTEALRRERADAMNVRRRTEEDKAKLG
                     DFYKAMILRELLPALDNLDRALKHYKRAWDLDDEKHEWIKGLRKVREQFEDCFAKLGV
                     QRIKTVGEPFDPKLHEAISVEDAGGQVEIVCEELQPGYRIGDEIIRHALVKVKMEKK"
     gene            complement(1078..1425)
                     /locus_tag="VEH48_00535"
     CDS             complement(1078..1425)
                     /locus_tag="VEH48_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17892.1"
                     /translation="MSDAERIDTTGIRWPIVPYLNPEDHEEDLFAGKAQFDEHPEMRE
                     LIDLGLDLLTAIMDGREGLNDLDRRYRERAIELCAELGIEDPDSWSLAASLAASHIWN
                     EHIAGGLRGSHVE"
     gene            complement(1418..2113)
                     /locus_tag="VEH48_00540"
     CDS             complement(1418..2113)
                     /locus_tag="VEH48_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17893.1"
                     /translation="MTDRQAKILQAIIEQYAEVASPVGSNLLARLFDVSSATIRAEMA
                     DLERMGYIDQPHTSAGRVPTDKGYRFYVNNLSEIGEKPAANRALTARVQPGGQPELTI
                     RNTVDTLVELTHNLGLATIGNELYMSGLSNLFGQPEFVHPAQVQEVARLLDNLQPWLY
                     EAAPNKPLSVFIGHENPIGRSAGASLIISRFRSPFSDRSYIGVLGPTRQSYRDVMGLV
                     GRAGKALEETLYV"
     gene            complement(2174..2866)
                     /locus_tag="VEH48_00545"
     CDS             complement(2174..2866)
                     /locus_tag="VEH48_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17894.1"
                     /translation="MDDTHSRLLDLLRHYKMPQAAAELLTAHPPLLVAGTTASGKDAV
                     ENFIQGISDWRHVVTHTTRQIRPGEVNGSDYWFVSEAEMLNLAEGQAFIEIKLIHDRQ
                     ISGTSIQAYNAVLAAGRKPMLRIDIQGVRDLRTRVPGLRPYFILPPSFDVWMERLDKR
                     GHMSHVERVQRLHSARLELETAIEDEHFILLINDEIARVAQEIVDGVTDIPSQQRNRQ
                     LARQLIDHIKAY"
     gene            complement(2872..3555)
                     /locus_tag="VEH48_00550"
     CDS             complement(2872..3555)
                     /locus_tag="VEH48_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17895.1"
                     /translation="MTRDEFHKQFPKLKQNYMPNVDVSRHIAQLTLLMTIGPSGVGKS
                     AVMKQLDYTYVPSDTTRLQTESEIDGMDYIFRQDYDQIFSEIQNGQFVQFVIGPAGEF
                     YGTKDSSYPSFGIATMAVVAKEVKNFRKLGFGQTISAFITPQSFEEWMRRFDNYRSLT
                     PEKRERRLVEGGQSLALSLSDSDTHFILNDEIDDAVRQIQKLVAGESDVERENTARRA
                     AREMLDGIN"
     gene            complement(3589..5364)
                     /gene="lepA"
                     /locus_tag="VEH48_00555"
     CDS             complement(3589..5364)
                     /gene="lepA"
                     /locus_tag="VEH48_00555"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXY17896.1"
                     /translation="MTQDHIRNFCIIAHIDHGKSTLADRLLELTGTVAKRDMKAQLLD
                     KMDLERERGITIKLAPVRMKYKDYELNLIDTPGHVDFSYEVSRSLAAVEGAILVVDAS
                     QGVQAQTLANVYLALAAELKIIPVLNKIDLPAADVGRTGAEVVSLLGCKKEDILQISA
                     KTGAGVEELLEKIISDIPPPAGKPEAPARALIFDSYYDDYRGVILYVRMVDGSINKND
                     GIKMLATSAQGLALEVGSLSPEMNPTAGLTSGEIGYIVTNLKTTREAKVGDTVTTTQE
                     KVQPLPGYKNVRPFVYAGFFPESNENYQALKDAVERLGLSDSALQYVPENSPVLGFGF
                     RLGFLGLLHMDIVRERLEREYNLELIITNPSTDYKVKQTSGKQLDIKSAAELPDASQI
                     AEISEPWIKGEIVAPSQFVGAVLQLIVSARGRQKHISYMDNLAVIDFEAPLANLLTDF
                     YDQLKSVTSGYGSFNYELADYRPEDLVRLDFYVAGDKVDALSIMVHRSEAERLARSAL
                     AKLKDVIPRQQFKVSLQAAIGGKFIAREDISSFRKDVTIGLYGGDVSRKKKVLAKQKR
                     GKERLKRFGKVDIPAEAFAVLLKRD"
     gene            complement(5388..6953)
                     /locus_tag="VEH48_00560"
     CDS             complement(5388..6953)
                     /locus_tag="VEH48_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HXY17897.1"
                     /translation="MSLTSAAALLIGASLFGQLLGFMRTQVVNGNFNTPNHVTTDAYF
                     AAFKIPDLFFYTIAAGALGVAFMPFLAEKLHQGDRRSVWRLTSSLLNLLGIIMAVVGV
                     VLFVFAPQMIRLVIGHNLSPQQTHDAVTIMRIIAFNPLLFTISGILTSLQQTFGRFFF
                     YALAPILYNGCIIASVYVFKNSIGLVGLGVGALAGAILQLLIVLFGLYGMRFRYEPVI
                     VLRNPDFRRILRNLPPRSIDQGMDSINSIVETNRAAVLGQGNITFYENAYTLHLVPIL
                     LIGTTIATAAFPRLNDRLAQGRPDLFKRDFLQVLRAMIWIILPIAVVAFFARAYFARL
                     IYKNIAPEIALILGFLVGAIVFRTLYAIISRYFYSNKDTRTPLFVSLFAIALNIYLAF
                     TLARPIVVGGYGVAGLAMAESIVATVEVFILVLIMVWRDHHLFTAQFWSGVIRTLSVT
                     GFTLITTYVMVNLVPLEASDRGFIILGTKILAVIIPTFIVHFAISSLFGLEEAQPVID
                     KIKQITLKPIRIQ"
     gene            complement(7211..8311)
                     /locus_tag="VEH48_00565"
     CDS             complement(7211..8311)
                     /locus_tag="VEH48_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXY17898.1"
                     /translation="MPKWLFTVLVFAKISVKRYFRDRLAIFFTVAFPLIFLLVFGALS
                     NGGGVSFHIGLINQSGSAFAQKFVKDTNASKMFKVDNSVTTLAQAKDKMSKSEIDATL
                     VLPPSFGVPKNGVPSGQALVYYDENNYDAAQTIESVIQGELEGINTQLIKISPPLSVT
                     SKSTNAQGLTKMDYTVSGLLGFAIIGLGIFGPVNYFPEMKKQGVLRRLHITPLRVWQF
                     FVSSVLSNAVIGLFSIAIMLLVASVAFGFHMVGNYFELAVFIILGILSIFGIGLAIGG
                     WAKNEQQAAPLANVIVFPMMFLSGTFFPRYAMPVWLQHVSAYLPLTPIIDGIRMIATE
                     GDHLTQIGPQLGWVVGWTVVIYAIAFRVFRWE"
     gene            complement(8313..9077)
                     /locus_tag="VEH48_00570"
     CDS             complement(8313..9077)
                     /locus_tag="VEH48_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXY17899.1"
                     /translation="MAEAILKVDGLTKSYGGRKVVKGVSFAVRTGEVFGILGPNGAGK
                     TTTLEMIETLRPIDAGTAVINGVNVSDNPQKVRYMIGVQTQSTAFMDKVKLTELLEQQ
                     AAAYGEKVDVLELLRDVNLENKAGSYIEDLSGGQKQRFSIAAALVHQPGIFFMDEPTT
                     GLDPQARRNLWSLVQKINDGGKGVTIILTTHYMEEAEVLCNRVAIMDEGRIVAMDTPK
                     ELIQHLLRSGFKKAHHVEQANLEDVFIDITGKKLGN"
     gene            complement(9170..9367)
                     /locus_tag="VEH48_00575"
     CDS             complement(9170..9367)
                     /locus_tag="VEH48_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17900.1"
                     /translation="MAYKHTNSKGVTYYLNSKKVTLRGGKVQTIYYFSKDERKDTGVD
                     LPNDRTVNENPRNGFLTLKRK"
     gene            9636..>10064
                     /locus_tag="VEH48_00580"
     CDS             9636..>10064
                     /locus_tag="VEH48_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17901.1"
                     /translation="MPPSQQIHQPQAAHPGSGRHRHIDWAGRAVRIEWFIFTSLIAVI
                     LLVVIGWMIYNNPTSSSESKLVDKSKYQAVFLNGGVTSGSVAYSTYFGHVTKLNDNYL
                     VLDNVYYLTDQSTTSGSSNPQLVKLGCQQLHSPYDEMVINR"
BASE COUNT         2329 a         2686 c         2559 g         2490 t
ORIGIN      
        1 gttggtgacg cgctggcggt tggccacctg cccgaccagc cgctcgccat ttttgttgat
       61 agctaccacg ctgggcgtgg tgcgcccgcc ctcgctgttg gcgatgattt ccggcttgcc
      121 agactgcatc gccgccatgg cgctgttagt tgttcctaaa tcgattccta tgattttagc
      181 catcttattc ctccttcttt tttaagctgc ttcttcgaat ttataaccca ggaagggcac
      241 ctcagggtcg tcgacatatt caacagttcg tccatgatcc cagccgcccg taacgcccac
      301 catgcctatt cctctcatga ccgcccagtt ttgcatgtct ccctcggcta gactttgtgc
      361 ccggaatctt agcgcatctt ccggtgtagt ttgcatggcc tcacccgttt taagaacggt
      421 gcccgagcta tgttcttcga ccgggatttc caactccccg ttctccatct gcctgcggag
      481 ttcagcctca atctctgctc tacgtttttc taaagcagca gtccacgctt ccaaatactc
      541 aggatccgtg gttgagacac tgcgattctg tggattgata tggattctta acgtttcttc
      601 agtagccatt actttttctc cattttaact ttaactaatg cgtggcggat gatttcgtcg
      661 cctatgcgat agcccggttg aagttcctcg caaacaattt caacctggcc gccagcgtct
      721 tctacgctga ttgcttcgtg cagtttgggg tcaaacggct caccaacggt tttaatgcgc
      781 tggaccccaa gcttggcaaa acaatcctca aactgctccc tgacctttct aagtccttta
      841 atccattcat gcttttcatc gtctaagtcc catgctcttt tgtagtgctt aagcgcgcgg
      901 tccagattat ccaatgccgg cagcagctcg cgcagaatca tggctttgta aaaatcgccc
      961 agcttagctt tgtcttcttc tgtgcgacgg cggacattca tggcatcggc ccgctcgcgc
     1021 cgcagcgctt cggtcagctc agcgacttgc tgttgcaaat cttggatagt tggtttttta
     1081 ctcgacatga ctacctctta aaccccccgc aatgtgttca ttccatatat ggctagcagc
     1141 tagagatgca gcaagactcc agctatctgg atcttcaata cccagctccg cacatagttc
     1201 tattgctctt tctctgtatc ttcgatcaag atcattcaaa ccttctcgtc catccataat
     1261 tgcagttaag agatctaatc ctagatcgat taattctctc atctcaggat gttcatcaaa
     1321 ctgtgctttg ccggcaaaca aatcttcttc atggtcttcg ggattcaaat aaggaacaat
     1381 cggccatctg atgccggttg tatcaattct ctctgcatca gacatacagg gtttcctcca
     1441 gggctttgcc ggcgcggccg accagaccca tgacatcgcg gtagctttgg cgggtcgggc
     1501 ctagcacacc gatgtaactg cgatcagaaa acgggctgcg gaagcggctg atgatcaggc
     1561 tggcaccggc gctgcggccg attggatttt cgtggccgat aaaaacgctc aacggcttat
     1621 tgggtgcggc ctcgtagagc cacggctgca ggttatctag aagtctcgcg acctcttgga
     1681 cttgggccgg gtgcacaaat tcgggctggc cgaacaaatt ggaaaggccg ctcatgtaaa
     1741 gttcgttgcc gatagtggcc aggcctaagt tatgagttag ctccaccaaa gtgtccacgg
     1801 tgttgcggat ggtcagttcc ggctggccgc ccggttggac gcgggccgtc agggcacggt
     1861 tggcggcagg cttttcccct atctcggaaa ggttattgac atagaaacgg tagcctttat
     1921 ctgttggtac gcgcccggcg ctggtatgcg gctggtcaat gtagcccatc cgttccaaat
     1981 cagccatctc ggcgcggatg gtggcgctgg atacgtcaaa aagtctggcc agtaagttac
     2041 tgccaaccgg gctggcaacc tcggcatatt gttcaattat ggcttgtagg attttagctt
     2101 gtctgtctgt catattgcac tccaggatgc taagtgctag tttaccgaag ttagcactct
     2161 catgtcaaga gtgctaatag gctttgatgt ggtctatgag ctgacgggcc agctgccggt
     2221 tacgttgctg tgagggtata tcggtcactc cgtccacaat ctcttgcgcc acccgcgcga
     2281 tttcatcatt gatcagcaag atgaagtgtt cgtcttcaat agctgtctcc agctccagcc
     2341 gggcgctatg caaccgctgg acgcgctcaa cgtggctcat atgcccgcgt ttatccagcc
     2401 tctccatcca aacatcaaaa ctgggcggca atataaagta cggccggagg ccgggcacac
     2461 gggtccgcaa atcgcgaacg ccttggatgt ctatgcgcag catcggcttg cggccggccg
     2521 ccagcacagc attataagct tgaatgctgg ttccggaaat ttgacggtcg tggataagtt
     2581 tgatctctat gaacgcttgg ccttcagcca agttgagcat ctcggcctcg cttacaaacc
     2641 aataatcgct accgttaacc tcaccggggc gtatctggcg ggtggtgtgg gtgactacgt
     2701 ggcgccagtc gctgattcct tggatgaagt tctctacggc gtctttgccg gaggcggtag
     2761 tgccggctac gagcagcggc ggatgcgcgg ttaaaagctc ggccgccgcc tggggcatct
     2821 tatagtgcct tagcagatcc agaagacggc tgtgggtatc atccattttg actagtttat
     2881 gccgtccagc atttcgcggg cggcccggcg ggcagtgttc tcgcgctcta cgtccgattc
     2941 gccggccacc aatttttgaa tttggcgtac cgcgtcgtca atctcgtcat taagaataaa
     3001 atgcgtatcg ctatcggaca gactcagggc caacgattgc ccgccctcaa ccagccggcg
     3061 ttcgcgcttt tccggtgtca ggctgcggta gttatcaaat cgccgcatcc attcttcaaa
     3121 agattgcgga gttataaagg cactgatagt ctggccaaag cccagtttcc gaaaattttt
     3181 gacttccttg gcaactacgg ccatcgtggc aatgccaaat gacggatagg agctgtcctt
     3241 agtgccgtaa aattcgccag ccgggccgat aacaaactgc acaaactggc cattttggat
     3301 ttcactgaaa atctggtcgt agtcctgacg aaagatgtaa tccataccgt caatttcgct
     3361 ctcggtttgt agtctggtgg tgtccgaggg cacgtaagta tagtccagct gtttcatgac
     3421 tgcgctcttg ccgactcccg aaggaccgat ggtcatcagc agagtaagct gagcaatgtg
     3481 ccttgataca tctacattgg gcatgtagtt ctgtttgagt ttgggaaatt gtttgtgaaa
     3541 ttcgtctcgt gtcattggct atagagtacc atcctgcaat gagaggggct agtctctctt
     3601 gagcaggacg gcaaaagcct cggcaggaat atctactttt ccaaaacgct tcaggcgctc
     3661 cttaccgcgt ttctgcttag ccagcacttt ctttttgcgg ctgacatcgc cgccgtacag
     3721 cccgatggtc acatctttac ggaaagagct gatatcttcg cgggcgatga atttgccgcc
     3781 gatggccgcc tgcaggctga ccttgaattg ctggcgcgga ataacatcct tcagtttggc
     3841 gagggcgcta cgggccagcc gctcggcctc tgagcggtga accatgatag ataatgcgtc
     3901 aactttgtcg ccagccacgt agaaatccag ccgcaccagg tcttccggcc ggtagtccgc
     3961 cagctcgtaa ttgaatgagc catagccgct ggtgacgctc ttgagctggt cgtaaaaatc
     4021 ggtcagcaaa ttggccagcg gcgcctcaaa atctatcacc gccaggttat ccatatagct
     4081 aatgtgtttt tgccggccgc gggcactgac aatcagctgc aagacagcgc cgacaaattg
     4141 gctgggggcg actatttccc ctttaatcca tggttcacta atttcagcga tttgactagc
     4201 atccggcagc tcggcggccg atttgatatc cagctgctta ccagaagttt gtttaacttt
     4261 gtaatcggta gaagggttgg tgatgataag ctccaggttg tactcgcgct ccagccgttc
     4321 gcgtacgatg tccatgtgca gcaggccgag gaaacccaga cggaagccaa agcccagtac
     4381 cggcgaattt tccggcacgt attgcaacgc tgaatcgctg agaccgagcc gctcaacggc
     4441 gtctttcagg gcctgataat tttcgttact ttccggaaag aacccggcat agacgaacgg
     4501 ccggacattc ttgtagccag gcagcggttg gactttttcc tgcgtagtgg taaccgtgtc
     4561 tccgactttg gcctcacggg tggttttgag gtttgtgacg atataaccga tttcgccgga
     4621 cgtcagcccg gcggttgggt tcatctccgg gctcaggctg ccgacctcca gtgccagtcc
     4681 ttgcgccgaa gtggccagca tcttgatccc atcgttctta ttgatgctgc cgtccaccat
     4741 acggacgtac agaatcacgc cgcggtagtc gtcgtaataa gaatcaaaaa ttaaagcccg
     4801 ggccggtgct tctggcttac ctgccggcgg cgggatgtca ctaataatct tctccagcag
     4861 ctcctcgacg ccagcaccag tcttggcgct aatttgtaaa atatcttctt ttttgcagcc
     4921 cagcaggctg acgacttcgg cgccggtgcg cccgacgtcc gccgccggca agtcaatctt
     4981 atttaagacc ggaataattt taagctcggc cgccaatgcc agataaacgt tggccagcgt
     5041 ctgcgcctgc acgccctggc tggcatccac tactaagatg gcgccctcga cagccgccag
     5101 actgcggctg acttcgtagc tgaagtccac atgccccggc gtatcaatca agttgagctc
     5161 gtaatctttg tacttcatac gtaccggcgc cagtttgatg gtgattcccc gctcgcgctc
     5221 taaatccatc ttgtccagca gctgggcttt catatcgcgt tttgctaccg tacctgtcag
     5281 ctccaataac cggtcagcta gcgtcgactt gccatggtca atatgcgcaa tgatacagaa
     5341 gttcctaatg tggtcctgag tcattaaccc tattgtaaca gaggtggcta ctgaatacgt
     5401 atcggcttaa gggtgatttg tttaatctta tcaatgaccg gttgggcttc ctccaggcca
     5461 aacagcgatg aaatcgcaaa atggacaata aatgtcggta tgatgacggc caggatttta
     5521 gtccccaaga taataaagcc gcggtcggag gcctccagcg gtaccagatt aaccattacg
     5581 taggtggtta tcagagtgaa gccggtgacc gacaaagtcc gtataactcc gctccaaaat
     5641 tgggcggtaa aaagatggtg atcacgccag accattatca aaaccaatat aaacacctcg
     5701 accgtagcga cgatggattc ggccatggcc aacccggcga caccgtaacc gcctacaaca
     5761 atcgggcggg ctaacgtaaa agccaggtaa atgttgagcg cgatggcgaa cagcgacacg
     5821 aacaacggtg tcctagtatc tttattagaa tagaagtaac gggagatgat ggcataaagc
     5881 gtccggaaaa cgatcgcccc gaccagaaaa cccagtatca aggcgatttc cggagcgata
     5941 ttcttgtaaa ttaagcgggc aaaataagcc cgggcgaaga aagctacgac agctataggc
     6001 aaaattatcc agatcatcgc ccgcaggacc tgcaaaaaat cacgtttaaa cagatcgggc
     6061 ctgccttgcg ccagcctgtc attaagcctc ggaaaggccg ccgtagcaat ggtggtgcca
     6121 atcaatagta tcggtaccag gtgcagcgtg taggcgtttt cataaaacgt gatgtttcct
     6181 tggcccagca cggcagcccg gttggtctca acaattgagt taatggagtc catgccttgg
     6241 tcaatggacc gcggcggcag gttgcgcaaa atccggcgga agtccggatt gcgcaggacg
     6301 ataaccggtt cgtagcggaa acgcataccg tacaggccga aaaggactat cagcaactgc
     6361 aaaatagcgc cggccagcgc cccaacgccc agtcccacca gccctatgct atttttgaaa
     6421 acataaacac tggcgataat gcagccgtta tatagtatcg gcgccaaggc gtaaaagaaa
     6481 aacctgccga aggtctgctg caagctggtc agaatacccg atatagtgaa cagtagcgga
     6541 ttaaaggcga tgatgcgcat tatggttacg gcgtcgtggg tctgttgcgg gctgaggttg
     6601 tgaccgatga ccagtcgtat catctgcggt gcaaaaacaa acagtacgac gcccacgaca
     6661 gccatgatga taccgagtaa gttaagcagg cttgaggtca ggcgccacac cgaacggcgg
     6721 tcgccctgat ggagtttctc ggccagaaag ggcatgaagg ccacgcctaa agcaccggcc
     6781 gcaatggtgt agaaaaagag atcgggaatc ttaaaggccg caaagtaggc gtccgtggtg
     6841 acatggttcg gagtattgaa gttaccattt accacctggg tgcgcatgaa acctaacagc
     6901 tggccgaaca gcgaggcccc gatcagcagc gccgcggcgc tagtcagaga cactctttgg
     6961 ttagctctat tgagccatct attcatcttg catttagtat aaaggctagc ggttagcggt
     7021 taaagcttaa aaatacttga ctagtcaagt ttttgactgt tgagcgagcc tataaaatct
     7081 gtcgggactc gagcgaaccc taactaagcg agtacctttc taaaaagaac gtgagctgcc
     7141 cagtgcagcc ggaggactat acctatgtcc cccgcgcgga acgggttctt tttagaaacg
     7201 gtacgagcga tcattcccag cggaaaaccc gaaacgcgat tgcataaata accaccgtcc
     7261 agccgacaac ccaccccagc tgcgggccga tctgtgtcag atgatcgccc tcggtagcaa
     7321 tcatcctgat accgtcaatg atgggcgtca gcggcaggta ggcgctgacg tgctgcagcc
     7381 ataccggcat ggcgtagcgc ggaaagaacg tgccggacag gaacatcatc gggaaaacta
     7441 tgacattggc cagtggcgcg gcctgttgct cgtttttagc ccagccgccg atggccagac
     7501 cgataccgaa gatggacaat atccccaaaa ttataaagac ggccagctca aaatagttgc
     7561 cgaccatgtg gaagccgaag gccacggacg ctaccagcag catgatagcg attgaaaaca
     7621 ggccgatcac agcgttggac agtacgctgg aaacaaaaaa ttgccaaacc cttagcggcg
     7681 taatgtgcag ccgccgcaat acgccttgct ttttcatctc agggaagtaa ttcaccggcc
     7741 caaagatgcc caggccgatg atcgcaaaac ccagtaaccc ggaaaccgtg tagtccatct
     7801 tggtcagtcc ctgggcattg gtggatttgc tggtcacact caagggaggg ctgattttga
     7861 taagttgagt gttgatgccc tccagctcgc cctgaatcac ggactcaatg gtctgggcgg
     7921 catcataatt attttcgtca tagtaaacca acgcctgccc ggaaggtacg ccgtttttgg
     7981 gcacgccgaa gcttggcggc aataccagcg tggcatcaat ctcgctcttg ctcatcttgt
     8041 ccttggcctg cgccagcgtg gtcacactat tgtcaacttt gaacatctta ctggcattgg
     8101 tatctttgac gaatttttgg gcgaaagcgc tgccgctttg gttgatcagg ccgatatgaa
     8161 aagacacgcc gccgccattg ctcagtgcgc caaacaccag aagaaaaatc agcggaaaag
     8221 ccactgtaaa gaaaatcgcc agacggtcgc gaaaatagcg cttgacgctg atcttggcaa
     8281 aaaccagtac cgtaaaaagc cacttcggca tgttagttcc ccaacttctt tccggttata
     8341 tcaatgaata cgtcttcaag gttggcttgc tcaacatggt gggctttctt gaaaccgctt
     8401 cttagcagat gctgtatcaa ttccttgggc gtatccatgg ctacgatccg gccctcatcc
     8461 atgatagcca cccggttaca aagtacttcc gcctcttcca tataatgcgt cgttaagatg
     8521 atggtcacac ctttgccacc gtcattaatt ttttggacca gactccagag gttgcgccgg
     8581 gcttgagggt ccaggccggt cgtgggttcg tccataaaga atatccccgg ctggtgtacc
     8641 agggcggcgg cgatggaaaa tcgctgtttt tggccgcctg agaggtcttc aatatagctg
     8701 ccggccttgt tttccaggtt gacgtccctt agcaattcca gcacatcgac tttctcgccg
     8761 taagccgccg cttgctgttc cagaagctcg gtaagcttga ccttatccat aaatgccgtg
     8821 gattgggtct gcacccctat catgtagcgg accttctgcg ggttgtctga tacatttacc
     8881 ccgttgataa cggctgtgcc ggcgtctatc gggcgcagag tttcaatcat ctccagcgtc
     8941 gtggtcttgc cggcgccgtt aggccctaga ataccgaaga cttcgccggt ccgtactgca
     9001 aagcttacac ccttaacaac cttacggccg ccataacttt tggtcaggcc gtctacttta
     9061 agaatcgctt ctgccactta aaattattat aacaaagagt aaagagggtt agtgccaaaa
     9121 tacccggctg ttttggccgg gtattctaaa ttttctggtt ggatttaaat tatttgcgct
     9181 ttaaggtcaa aaagccgttg cgcgggtttt cgttgacggt gcggtcgttg ggcagatcca
     9241 cgccggtgtc tttgcgctcg tccttggaaa agtagtaaat cgtctggact ttaccgccgc
     9301 gaagggtgac cttcttggaa tttaagtaat atgtaacgcc cttgctgtta gtgtgcttat
     9361 aagccatctc gtccctctct ccgttaggtt aatggtactc ctaacatact ccgtcagctt
     9421 ttcagttgtc aaccccttag ctatctatta atgtagtaaa atcaccaatg gttacccctg
     9481 ttatcgagct ttgatagcag ctgaaataac tagtgttaat ttgtgacggt ccaaggtccg
     9541 gcttgcatgc caaagcgctt atgcgttact atgtttaggt attaatctaa ggtgggataa
     9601 acacacaata aggattttca attagaggag gaaatatgcc cccatcacaa caaattcatc
     9661 aaccgcaagc ggcacacccg ggctccgggc gccatagaca catagactgg gccggccggg
     9721 ccgtacgtat tgagtggttc atttttacca gcctgattgc cgtgatactg ctggtagtta
     9781 tcggctggat gatttacaat aacccgactt ccagttcgga atccaagctg gtagacaaga
     9841 gcaagtacca agccgtgttc ttaaacggcg gcgtaaccag cggctcggtg gcatattcca
     9901 cctacttcgg ccacgttacc aaacttaacg ataactatct ggttttagat aacgtttact
     9961 acctgactga ccaatccacc accagcggca gcagcaatcc gcaactggtc aagctgggtt
    10021 gtcagcagct gcactcgcca tacgacgaga tggtcatcaa ccgt
//
LOCUS       DASPTF010000020         3355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1460193, whole genome shotgun sequence.
ACCESSION   DASPTF010000020 DASPTF010000000
VERSION     DASPTF010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460193"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VEH48_00585"
     CDS             <1..464
                     /locus_tag="VEH48_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17902.1"
                     /translation="FSVNHGGKTGSFFQSAPLNFVERLKNNGNVHLQPTGQVTITDMF
                     GRKLATLPVNVPPGNILPASIREFSQALDKSVIGNKKLFGHYTAKMSVTYGTDKKALN
                     SSLGFWVIPFKLVAGVIIVLIAGFFAIRYAIRRYNRYILEQANKRSGRQRK"
     gene            523..1497
                     /locus_tag="VEH48_00590"
     CDS             523..1497
                     /locus_tag="VEH48_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17903.1"
                     /translation="MYGPLKISQHQHSGRLRPHEHTSYLALAGLIIVAGLALMNSSLS
                     TVLADHPPPQAGSIGLTGTVPATPPNTAAVITSPTDQQHFSTSPITVSGTCPTGTLVE
                     IYKNDIFAGSAPCGSDGKFSMEIDLLYGTNSLTAQVYDVLNQAGPVSTAVTVYYNVSA
                     IQSAPLSLLNFEGTQLLLSTDSVYRGSFPDQNLNVPITIIGGTPPFAVNVQWGDANNK
                     VIPRGDNTIFNASHAYQRAGTYQIDLQAADSQQRVAFLTVAAIINGQPSVISASSNKS
                     STNKFLLLWPLYAIAATLVASFWMGERREKRILAATTTSQIPSSGLLQ"
     gene            complement(1529..1966)
                     /locus_tag="VEH48_00595"
     CDS             complement(1529..1966)
                     /locus_tag="VEH48_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17904.1"
                     /translation="MPSAKKYKSRRAAIETLRRFFAGKPDTDRLSVEEVFIATGRQKT
                     APRANMAWLSNKLTTLRYHNLIDSEYSYEGRRRLEAISLTLDGKRALGQIGGLAGTDS
                     GPSAAASPPLAGLSDALAIVSALQQQYPRFDIALTIKPKDSPQ"
     gene            2231..>3355
                     /locus_tag="VEH48_00600"
     CDS             2231..>3355
                     /locus_tag="VEH48_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17905.1"
                     /translation="MQPGSQKAKLWLFALGIFVGAFLALGLLKTPPAAASTGINQTIN
                     FQGRLLNAQGATVPDGYYNIEFKIYQDGDGQSAGDSTGSPSGSLKWTEDYLNQAGHGV
                     KVVNGYMSVNLGSITPFGSNINWNQNTLWLSMNIGGTGASCTPFSSCSPDGEMLPMQP
                     LTTAPYALNSGELGGLSSSQFVQLAQGVQTDTSNVTAIGVNKTGSGKIIDLQNNSTDV
                     FTVDTSGNTVGIGITGSTNANSTVHIADSSAGIQTVTIGSTSSSSATTIQAGSGGISL
                     GANTTLAANSNFTYASGSGGFDAHNSSGTFQTSTGANSLNGATTVTGTNSFTVNGGLS
                     SLKAGLSVNSGAAGTVGAIIQGASSQTAALLQLQDSTGANL"
BASE COUNT          802 a         1011 c          831 g          711 t
ORIGIN      
        1 agttttcggt caaccacggc ggcaagaccg gctcgttctt ccaatccgcg ccgttgaatt
       61 tcgtagagcg gttaaagaat aatggcaacg tccaccttca gccgaccggg caggtgacta
      121 taacggatat gttcggccgc aagctggcca ccttgccggt caatgtgccg ccgggtaaca
      181 tcctgccggc cagcatccgt gagttcagcc aggcgctgga taaatcggtc atcggcaaca
      241 aaaaactatt cggacactac accgccaaaa tgtcggttac ctatggtacc gataaaaaag
      301 ccttaaatag cagcctgggc ttttgggtga ttccgttcaa gctggtggcg ggcgtcataa
      361 tagtcctgat tgccggcttc ttcgccatcc gttacgccat ccgccgctac aaccgctaca
      421 tcttggagca ggccaacaag cgttcgggcc gacagcgcaa gtaaccgctg actccgtctt
      481 cttagtaacc ccttaaaaca caagtgttat ataattagtc tgatgtacgg tcctcttaag
      541 atcagtcagc accagcacag cggacgcttg cggccgcacg agcacacctc ttatctggca
      601 ttggccggcc tgataatagt agccggcctg gcgctgatga actcgtcgct atccactgtg
      661 ctggccgacc acccgccgcc gcaggccggc tctatcggcc tgaccggcac cgtgccggca
      721 acaccgccca acacggccgc tgtcatcacc agcccgaccg accagcagca cttctcaacc
      781 agcccgataa cggtctccgg tacttgcccg acgggaaccc tggtagaaat ctataaaaac
      841 gacattttcg ccggttccgc cccctgcggc agcgacggta aattctctat ggagattgac
      901 ctgctgtacg gcacgaacag cctgacggcc caggtttacg atgtgcttaa ccaggccggc
      961 ccggtatcaa ccgcggtgac cgtttattac aatgtttcgg ccatccagtc ggcgccgctg
     1021 agcctgttga actttgaagg cacccagctg ctgcttagta ccgactcggt ttaccgcggc
     1081 tcttttccgg accagaatct taacgtgccg ataaccatca tcggtggcac cccgccgttc
     1141 gccgtcaacg tccaatgggg cgacgccaac aataaagtca ttccgcgcgg cgacaacacc
     1201 atattcaacg cctcccacgc ctatcagaga gctggcactt atcagattga cctccaggcc
     1261 gccgacagcc agcagcgggt ggcttttcta acggtcgccg ccattatcaa tggccagcca
     1321 tcggtaatca gcgccagcag caataaatca tcaaccaata aattcttact gctctggccg
     1381 ctgtacgcca tcgccgccac gctggtggcc agtttctgga tgggcgagcg ccgcgaaaag
     1441 cgcatcctgg cggcgacaac cactagccag ataccgtctt ccggcttact ccagtagtta
     1501 aaacgagact ctatatctgt taataccgct actgcggcga atctttaggt ttgatggtca
     1561 gggcgatgtc aaagcgcgga tactgctgct gcagagcgct gactatggcc agagcatctg
     1621 ataagccggc tagcggcggg ctggcggctg cacttgggcc tgaatctgtg ccggccagcc
     1681 cgccaatctg gcccaaggct cgtttgccgt ctaaagtcag gctaatggcc tccagccgcc
     1741 gccggccttc gtaagaatat tcgctatcta tcagattgtg gtaacgcaga gtagtcaatt
     1801 tattggaaag ccaggccata ttggccctag gagcggtttt ttgccgcccg gtggctataa
     1861 atacctcttc aaccgataaa cggtcggtat cgggcttgcc ggcaaaaaaa cgccttaagg
     1921 tttctatggc tgcccggcgg gatttgtatt tcttagcgct cggcatcttc taatgtttaa
     1981 tcttatcacg cattatttaa ttgttcaata atttagggta ggagagggaa atgagataat
     2041 acaaaagaat taaattaact ccttaaaacg ccgccagcgc tttttttgaa tacaaaactg
     2101 tggataacgg ccaaaaacac cttgtaaagg acgcgcgcgc aaactaaaat gataactgat
     2161 agagaactcc tgtaaaacaa aaaccgcggc tctctaagaa caaaaaccaa aagaaaaaca
     2221 gataaaaaac atgcaaccgg gctcccaaaa agccaagctc tggctgttcg cattaggcat
     2281 ctttgtgggt gcttttttgg cgttgggcct gcttaagacc ccgccggcgg ctgcttcaac
     2341 cggtatcaac cagacgatca acttccaggg aaggctgctt aacgcccaag gggcgaccgt
     2401 ccccgacggc tattacaaca ttgagttcaa gatctaccag gacggcgacg gccagagcgc
     2461 cggtgacagc accggctcac cgagcggttc gctgaaatgg accgaggact atctgaacca
     2521 ggccggccac ggcgtcaaag tggtcaacgg ctatatgtcg gtgaatttgg gctcaatcac
     2581 accttttggc agcaacatta actggaacca aaacactctc tggctgtcaa tgaacatcgg
     2641 cggcaccggc gcttcctgta cgccgttctc ctcctgcagc ccggacggtg agatgctgcc
     2701 aatgcagccg ctgaccaccg cgccttatgc tcttaactcc ggcgagctgg gcggtttgag
     2761 cagttcccag tttgttcaac tagcccaagg agtgcagacc gacaccagca acgttacggc
     2821 tatcggcgtc aacaagaccg gttccggtaa aattattgac ctgcagaata acagcaccga
     2881 cgtctttacg gtagacactt ccggcaacac cgttggtata ggcattaccg gctcaaccaa
     2941 tgccaattca acggttcata tcgccgactc ttcggctggc atccaaacgg ttaccatcgg
     3001 ttcaaccagc tcctccagcg ctaccaccat ccaagctggc agcggcggca tctccttagg
     3061 cgccaataca accctggccg ccaactctaa ctttacttac gcttccggca gcggcggctt
     3121 tgacgcccat aattctagcg gaactttcca gacctctacc ggcgccaact cgctgaacgg
     3181 cgccaccacc gtcactggta ccaacagctt cacggtcaac ggcggcctaa gcagcctaaa
     3241 agccggtctg agcgttaact ccggagctgc cggcacagtt ggcgccatca tccagggagc
     3301 ttcttcccaa accgccgccc tgctgcagct tcaagactca acaggggcaa atctg
//
LOCUS       DASPTF010000021        11685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1462015, whole genome shotgun sequence.
ACCESSION   DASPTF010000021 DASPTF010000000
VERSION     DASPTF010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 11685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11685)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11685
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462015"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /gene="pilM"
                     /locus_tag="VEH48_00605"
     CDS             complement(<1..628)
                     /gene="pilM"
                     /locus_tag="VEH48_00605"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HXY17906.1"
                     /translation="MNLLSGISDFFGLDIGTTAIRVVQLKGSGPVKALDRYGETQLQG
                     TLALSDAKVDRQKVAQEIHNLVKQAGITTNNVAVNLPSSRVFTAVVDMNKMSEAELAK
                     TIRYQAQTFIPTPLARSKIDWAVIGPSPQDTAKVEVLLSSVPNDFIEDRLDMLEAAGL
                     DVVAFEPDSMALARAIIPSDSTTPEMILDLGASSTDLVITVAGVPRLTR"
     gene            complement(692..1711)
                     /locus_tag="VEH48_00610"
     CDS             complement(692..1711)
                     /locus_tag="VEH48_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17907.1"
                     /translation="MKLAAAKIRLLYAGAAVLGLFFSLTPLVAADTYPYLRVTGGDVW
                     TGGWFESGGSCSNSAPPYQDNRDSGGGISTYLGASGGSSGDFGVYALGSIASGGSPTG
                     GFASDVKAGGGKTLTFANTGSPLGLFQGGTPQQHCLPDYYDAQSSTTSLNGAASVNLG
                     GLGSQQYLDSLSAGFVSVSGAIAAGQKTTLFVKGDVHITGNITYAAGYTESTVPKFAL
                     VVKGNIFVEPNVTQIDGVYVAQPSSGSSDGVIWTCTDVANSSSPWGPDIKKNCTNSLT
                     VNGALLAKQVNYLRINGDAGTAGSPAAETVNYPAANIVGGPFFNPPSTGSPVIQSLIS
                     LPPLF"
     gene            1790..1963
                     /locus_tag="VEH48_00615"
     CDS             1790..1963
                     /locus_tag="VEH48_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17908.1"
                     /translation="MSNGIISFLVSISASTWIYTKFMKYSGNNSKTAIIGAIISAIII
                     FFIFFSILNLVLK"
     gene            1966..2367
                     /locus_tag="VEH48_00620"
     CDS             1966..2367
                     /locus_tag="VEH48_00620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HXY17909.1"
                     /translation="MADNVFTKIIKGEIPSHKIYEDEYTFAFMDIRPLNPGHVLVITK
                     EEEDHLWDLEDELYQHLMAATKKIALRIREVLNPPRVGMNVEGFGMPHVHVHVYPLYK
                     GLESTMSDFLSKPDKKADDATLAEMAQRLAF"
     gene            2464..3315
                     /locus_tag="VEH48_00625"
     CDS             2464..3315
                     /locus_tag="VEH48_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXY17910.1"
                     /translation="MDEKTLGKRLQLARKRAGLTQQELCQKAGLSYSTLAKIERGAIR
                     SPSVFTVASIAAATGTAIEDLLGTPAQNQASPAPPAKKRSKTGVTFVYFDVNGTLVHF
                     LHKAFSDVARVCGQPADLVETVFWRYNDAANSGQITPEEFNATLGKELELPDFDWQKY
                     YMANVEPTPGIGEFLQWAAENYEVGILSNNIPGYIHELTTAGKIPRVAFSAIVDSSKV
                     KLIKPDPKIYEIAQQMAAAEPREILLVDNERANLIAADRAGWQTIQFDELEPEASLTR
                     LRQTLEF"
     gene            complement(3370..3795)
                     /locus_tag="VEH48_00630"
     CDS             complement(3370..3795)
                     /locus_tag="VEH48_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17911.1"
                     /translation="MQWEDLHRQARHVVLDLYGAKEPTAEDVAKLVDRLRSDRYDFAV
                     DLVNIADWLAMNPRDLLAALDIQDFDSPVANMGKLAIIARVRSTLSSFGHSGTLPEIL
                     LKTELDLRPAITKWLIEAAVGLNRLRAQEIGTEVLRYLQ"
     gene            complement(3996..4379)
                     /locus_tag="VEH48_00635"
     CDS             complement(3996..4379)
                     /locus_tag="VEH48_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17912.1"
                     /translation="MPRLVLVVAELPQTPACIVALVTNELEYATHRDVRIEESESGLR
                     YPILVETDITLPAWDKQLDEPIGKLVEGFDWLLRWLPDICEAESHRIGLTLQGPWDSR
                     WAWKERELNQAQRLSAACYRELAGI"
     gene            complement(4613..4984)
                     /locus_tag="VEH48_00640"
     CDS             complement(4613..4984)
                     /locus_tag="VEH48_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17913.1"
                     /translation="MSFLDELAKAAHKIVWAAAIEALQSGETKIARFEIQVDAGSDTL
                     PEALSLTESIYGIYTHDYEAFILSPSRLMAYVLKSIATGASMYMQDEEDVFDGAHPRA
                     SLELVRLAIELAKIEHQKTIA"
     gene            5158..5904
                     /locus_tag="VEH48_00645"
     CDS             5158..5904
                     /locus_tag="VEH48_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXY17914.1"
                     /translation="MFNQLQALDLSPAERTAYETLITHGPMAPPQLAKAANLTRVNSY
                     AALRGLSQKELAAEKDVNKKRVYSPAPPTKLQELARQKTEVAKASQESIDAIIPTLMN
                     QFALVSGQPGISHYESLDGIKQIYEDSLRPPHHQESLVLRSIYDSKEIEPYILKYIRR
                     RAKLGIKNRMLTPYFDTSLDDKALLRERRFLPKEKFSLPTEISIYGDKVSLVSLRKDL
                     IGSVIQSKDVAQTFRILFELLWSQASTEKI"
     gene            complement(5901..8537)
                     /locus_tag="VEH48_00650"
     CDS             complement(5901..8537)
                     /locus_tag="VEH48_00650"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXY17915.1"
                     /translation="MKLAKAYEPNRYEADIYKLWEDSGVFKADPNSGKEHFSVSMPPP
                     NETSTLHMGGALFLTLQDIMVRHARQLGKDVLWLPGTDHAAIATNAVVERQLAEQGTD
                     KHQIGREAFIAKVKEFAGGNRETMISQMRAMGASCDWSRFRYTLDDSLNRSVNEVFNK
                     MYQDGLIYRGHRIVNWDPKLETTVSDDEVDYVEEKAPLYTLQYGPFQISTARPETKFG
                     DKYVVVHPEDKRYKNYSDGQTFECEWLNGKVTATIVKDEAVNPEFGTGVMTITPWHDR
                     TDFEIAERHGLEREQIIDYKGKLLPIAGEFSGMDIEEARPKIVDKLKAKGLLVKVDED
                     YTHNIALNSRGKGVIEPQIMLQWFVDVNRPAVDWKGQMRSFKEVMRAVVEDGDIRIIP
                     KRFEKIYYHWIDNLRDWCISRQIWWGHQVPVWYRQAQGDRAEGVGDSEETYVGTQAPE
                     GQGWKQDPDTLDTWFSSALWTWSTLIDPKLAADHSLSLDQLLEKSVDYRTYHPTSVME
                     TGWDILFFWVARMILATTYVTGQIPFRTVYLHGLVRTEEGKKMSKSDPDTIVDPLDMI
                     AKYGTDALRLALVSGTGAGNDQRLGLAKIVANRNFCNKLWNIARYVEDAVGEDSREGA
                     VPKSIADHWILNKLSIFSQQLGEDLTNFRFAEAYDKLYHFVWDDFADWYIEASKVELN
                     APLLAYVLEQTLKLTHPFAPFLTETIWRELGWQNSMLISQPWPEICEFEAQEAEDFQL
                     ILQLTIWSRAASTLADFVRPNLFFMESSLTLKDNANLLKRLARLKSVDETDTEKNSFI
                     SIEFPAQGIKVWLDIDVAKYRSALENQLKEVQGEESILRTRIENPEYKEKAPKELVEQ
                     TEKALADASQRAEQLQKILQNL"
     gene            complement(8830..9897)
                     /gene="tsaD"
                     /locus_tag="VEH48_00655"
     CDS             complement(8830..9897)
                     /gene="tsaD"
                     /locus_tag="VEH48_00655"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXY17916.1"
                     /translation="MRILGIETSCDETAAAVVEDGRKMLSNVVASSVDLHAAYGGVVP
                     EIAARSHIEAIIPVIDEALKTAKTDWGGIDAIGVTYGPGLAGSLLIGVLTARTLAIAK
                     NKPLYAVNHLEAHVYATFITKVSPALATIYSLPAAAPKFPILALIVSGGHSQLVLFKN
                     HFDYRVLGQTGDDAVGEAFDKVAKILGLPYPGGPSVSQAAKKGDPASVKLPIAKLDNP
                     YDYSFSGLKTAVLRAAQQMAGKDFTFPSTGLPKVLKAKQKADLAASFQATAIASLTDG
                     LKMAIEVYHPKSVVIVGGVAANQALRRAAAKLSPTPVYPDIKLCTDNAAMIAVQAYYQ
                     ASRKRPPADPYSLEINPSLQM"
     gene            9921..10202
                     /locus_tag="VEH48_00660"
     CDS             9921..10202
                     /locus_tag="VEH48_00660"
                     /inference="COORDINATES: protein motif:HMM:NF019481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HXY17917.1"
                     /translation="MADLKAGVYRHYKGLLVQVLGTARHSETDEKFVAYVPLGVKAGP
                     RITVRPYNMFFEEVEIEGKKQLRFEFIGETVSEEIAKDYDPYSQRRATL"
     gene            10199..11488
                     /locus_tag="VEH48_00665"
     CDS             10199..11488
                     /locus_tag="VEH48_00665"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXY17918.1"
                     /translation="MNSRKVVKSLIPKKLFEKVEPAGHGVEAALVNAKNRWPARGLNV
                     IGVTGTDGKTTTCMLIAKMLRNSGQKVAMITTVAVDYADGQGERPSPTHLTTASAGQL
                     QAMFKKIKANQPDWVVLEVSSHALAQYRVLGVPFSVAVMTNLTHEHLDYHKTFARYRD
                     AKVRLFKLAARNHRGLRTGVINADDPSAEYFIRAVPRSVTYGIKNGALQAKILKTTAA
                     GSEFQVGGLAINLNLPGEFNIYNALAAIGAGQVIGLSDEQIGQGIAALKAVPGRMMRL
                     EAGQRLEVLIDYAVTPAALENVLKAARKTTKGKVMLVFGATGDRDKAKRPVMGEVAAK
                     FADRIFLTDDETYREDPAAIRQAVMTGIAKAGGQAKTTEVNERAAAIKQAIAKAKAGD
                     TVLITGLGHQKDRNMGGKLIPWSDSEIAKQLLNRQHR"
BASE COUNT         2737 a         3282 c         2917 g         2749 t
ORIGIN      
        1 cccgtgtcag gcgcggcaca ccggccacgg tgattaccaa atccgtggag ctggcaccga
       61 gatccagtat catctccggt gtcgtggaat cgctggggat aatcgcccgt gccagcgcca
      121 tgctgtccgg ttcaaaagcc acgacgtcaa gcccggcggc ctccaacata tccagccggt
      181 cttctatgaa atcgttgggt acgctggaca gtaggacctc taccttagct gtgtcttgcg
      241 gcgaaggccc gataaccgcc caatctatct tggagcgcgc cagcggcgtg ggaataaagg
      301 tctgcgcttg gtaacggatg gtcttggcca gctcggcctc gctcatctta ttcatatcaa
      361 ccaccgctgt aaaaacccgg ctggacggca ggttgaccgc cacgttgttg gtagtgatac
      421 cggcctgctt gaccaggttg tgaatttcct gggcgacttt ttgacggtcc accttggcgt
      481 cgctgagcgc cagcgtgccc tgcagctgcg tttcgccgta acggtccaga gccttgaccg
      541 gcccggaacc tttgagttgc actactctga ttgccgtggt gccgatatcc agcccgaaga
      601 aatcagatat cccactcaga agattcataa ttatttccga ttatatcact taaacataag
      661 aacaaactac cgcgattttg ctttttagcg cttaaaataa cggcggcagg ctgatcagac
      721 tttggataac aggactgccg gtgcttggcg ggttaaagaa cggcccgccg acaatattag
      781 ccgccggata gttcacagtt tcagcggccg gtgagccggc agtgccggcg tcgccattga
      841 ttcgcaggta attgacttgt ttggccagca gtgccccgtt gaccgtcaag ctgttggtac
      901 aattcttttt tatatccgga ccccaaggcg aagaactgtt ggcaacgtcg gtgcaggtcc
      961 aaattacacc gtcgctgctg ccgctgctgg gctgggcgac atagacgccg tcaatctggg
     1021 tgacattcgg ctcaacaaag atgttgccct tcactaccag ggcgaacttg ggcacagtgg
     1081 attccgtata gccggcggcg taagttatgt tgccggtaat gtgcacatcg cctttgacaa
     1141 acagcgtggt tttttgcccg gcggcgatag cgccggaaac actgacaaat ccggccgaaa
     1201 gactgtcaag atactgctgg gagcccaggc cgccaaggtt aactgatgcg gccccgttga
     1261 gactggtcgt tgaactctgc gcgtcgtaat aatccggcag gcagtgctgc tggggtgtgc
     1321 cgccctggaa cagcccgagg gggctgccgg tattggcaaa ggtcagcgtc ttgccgccgc
     1381 cggccttaac gtcgctggca aaaccgccgg tcggagagcc gccgctggcg atgctgccca
     1441 gggcgtaaac gccaaaatca ccgctggaac cgccgctggc gcccaagtac gtggaaattc
     1501 cgccgccgct gtcccggtta tcctgataag gtggcgcgct gttgctgcag ctgccgccgc
     1561 tttcaaacca gccgccagtc cagacatcgc cgccggtaac tctaagatag ggataagtgt
     1621 cggctgccac cagcggggtt aggctgaaaa acaaacccag cactgcagcg ccagcgtata
     1681 gcagtcgtat cttcgcggcc gcgagtttca tacataagca gtatacaata atccgccgta
     1741 ttttgggcaa gcgccgtcta atctccggta tttttgctat catggtgaga tgagcaacgg
     1801 tatcatcagt tttctggttt caatcagcgc cagcacctgg atctacacca aattcatgaa
     1861 gtacagcggc aataacagca agaccgccat catcggcgcc ataatttccg caatcatcat
     1921 cttctttatc ttcttttcca tcctcaacct ggtcctcaaa tagccatggc cgacaacgtc
     1981 ttcaccaaaa tcatcaaagg cgaaattccc tcccacaaaa tttatgaaga cgaatacact
     2041 ttcgccttca tggatatccg gcccttaaat cccggacatg tcctagtaat cacgaaggaa
     2101 gaggaagatc atctctggga tctagaagac gaactttatc agcatctgat ggccgcaacc
     2161 aagaaaattg ccctacgcat cagagaggtt ctgaatccgc caagagtggg catgaacgta
     2221 gagggttttg gtatgccgca tgtccatgtc catgtctatc cgctttacaa gggtttggaa
     2281 tccacgatga gcgatttcct ttctaaaccc gacaagaagg ctgacgacgc taccctggcc
     2341 gaaatggccc agcgtctagc cttctagtac tagtccaaca ccttactatc ccctatcccc
     2401 tatcccctat cccctatttt ctggtactat atggctatat gaggtggagt tttgggcggc
     2461 gctatggatg agaagacttt aggcaaacgc cttcagctgg cacgcaagcg cgccggcttg
     2521 acccagcagg aactctgcca aaaggccggc ctctcctact ccacgctggc taagatcgag
     2581 cgcggcgcca tccgttcacc ctctgttttc accgtcgcgt caattgccgc ggcgacgggg
     2641 acggcaattg aagatttatt gggcacgccg gcgcaaaatc aagcttcccc ggccccgccc
     2701 gccaaaaaac gctccaaaac cggggttact ttcgtttatt tcgatgttaa cggcacttta
     2761 gtccattttt tacacaaggc tttcagcgac gtcgcccggg tctgcggcca gcccgccgac
     2821 ctggtggaaa ccgtcttctg gcgctataac gacgccgcta acagcggcca aatcactcct
     2881 gaggagttca atgccaccct cggcaaagaa ctggaactgc cggattttga ctggcagaaa
     2941 tattacatgg ctaatgtcga acctaccccc ggtattggtg agtttctcca atgggccgct
     3001 gaaaactatg aagtcggtat cctaagcaat aacatacccg gttacatcca cgagctgacc
     3061 accgccggca aaatcccgcg ggtagccttt tcggccattg ttgattcctc aaaagtcaag
     3121 ctcatcaagc cggaccccaa aatctatgag atcgcccaac agatggccgc cgccgaaccg
     3181 cgcgaaatcc tgctggtaga taacgagcgt gccaatctca tcgccgccga ccgcgccggc
     3241 tggcaaacca tccagtttga cgagcttgag cccgaagcaa gtctcacccg cctccgccaa
     3301 accctcgaat tttagataaa ttaataggcc ggagaggagc acgaatgctc aactctccgg
     3361 cctttctcgc tactgaaggt agcggagtac ctctgtacca atttcttgtg ccctcagacg
     3421 gttcagccct acagccgcct caatcagcca cttcgtaata gctggcctta gatcaagctc
     3481 agtcttgagc agaatttccg gcagcgtacc ggaatgacca aagctcgaca gagtgctccg
     3541 aactctggca atgatcgcca acttgcccat attggctaca gggctgtcga aatcctgaat
     3601 gtcgagcgca gccaacagat ctctcggatt catggcaagc caatcggcaa tgttgacaag
     3661 atcaacggca aagtcatagc ggtcactcct cagcctatcg acaagcttgg ccacgtcctc
     3721 ggctgtaggt tccttggcac cgtacaggtc gagcaccaca tgtctcgcct gacgatgcaa
     3781 atcttcccac tgcattattg cctcctcggt acgaatatcg atttttcaaa gtgctttctg
     3841 cgtttcgctg tttccagctc atcaggcgga gcattcactc cgcgacgcta aatgacaatc
     3901 acagctggcg ctaaacgcgc caagatgagg tctcgcccaa agttatagtg gccagcactt
     3961 cctttggatc ccctctcacg ccgtctctcg gcgacctaaa ttccggcgag ctcgcggtag
     4021 caggcagcgg acaagcgctg ggcttggttt agctcacgtt ccttccacgc ccaccgcgaa
     4081 tcccacggac cctgcagcgt caaaccaatt ctatggcttt cagcttcaca gatgtccggc
     4141 agccaccgca acagccagtc aaacccttcg acaagtttgc cgatgggttc atccagctgc
     4201 ttatcccagg caggcaacgt gatatccgtc tctacaagaa tcggatatct cagaccgctt
     4261 tcgctctcct cgattcggac gtcgcgatgc gtcgcgtatt ccaactcgtt cgtaactagc
     4321 gctacgatac aagccggcgt ctgcggcaac tcggcgacga ccagaacgag gcgaggcaac
     4381 gcgctctcga aacgtgccgc ccggaattct ccaggcttag gtttagccat ctcacctcct
     4441 tttaaaagtg atcgctattt accaaactca ttacgagcct gggaatgcgg acggccaaat
     4501 ttgctgtgat tgtcaatgta cttgcgattc gtcctcttgg actcatcagg gcggatacac
     4561 atccgccgtc gcttatgggt gggagctagt gctcccaccc ttaccgagag ttttatgcga
     4621 ttgtcttttg gtgctctatc ttggccaatt cgatggcaag acggacaagc tcgagagacg
     4681 ccctcggatg tgcgccgtca aagacgtctt cttcgtcctg catgtacata ctggcgcccg
     4741 tagctattga cttgaggaca tatgccatta acctggatgg tgacaaaatg aacgcctcgt
     4801 agtcgtgcgt atatatgcca tatatggact ccgtaagcga cagtgcttcc ggcagtgtgt
     4861 cgctcccggc gtcaacttgg atctcaaacc tggcaatctt cgtttcacca gactggagcg
     4921 cctcaattgc cgccgcccaa acaattttgt gggccgcttt ggcaagctcg tcaaggaagc
     4981 tcaagcttac tcctctcgat agtggtttta aggttcaggt tgatgacgtt ctatcttcaa
     5041 cctgcgacat aatacactaa tgcttagagt ttgtcaagac ttttttatca ttttatttag
     5101 cactgactat agataagtat ttacttttta tgtttaattt gttagactag aattaacatg
     5161 tttaatcaac ttcaagcact cgatttatcg cccgctgaac gcacagcgta cgagactttg
     5221 ataactcacg gccccatggc gccacctcaa ctggccaagg ccgctaacct gacccgcgtc
     5281 aacagctacg ccgccctgcg cggcttgtcg caaaaagagc tggcagcaga gaaagatgtt
     5341 aataaaaaac gtgtttactc tcctgctcca ccgaccaaat tacaggagtt agcccgccaa
     5401 aaaaccgagg tcgccaaagc cagccaggaa tctatcgacg ccattatccc gacgcttatg
     5461 aaccagttcg ccctggtctc cggccagccc ggcatcagcc actacgaaag cctggacggt
     5521 atcaagcaaa tctacgaaga cagcctgcgc ccgccgcacc accaagaaag cctggtgctg
     5581 cgctccattt acgacagcaa agaaatcgag ccctatattc tgaaatacat cagacgtcgt
     5641 gccaagctgg gtatcaaaaa ccgcatgcta acaccctatt ttgatacctc cttagacgat
     5701 aaagccctgc tgcgcgaacg ccgtttcttg cccaaagaaa aattctcgct gccgacagag
     5761 attagcatct acggcgacaa ggtttccctg gtcagtctgc gcaaagacct gatcggcagc
     5821 gtcatccaaa gtaaggacgt cgcccaaacc ttccgcatcc tattcgaact cctttggtcg
     5881 caggcctcaa cagagaaaat ctagagattt tgcaagattt tttgcagttg ttcggcacgc
     5941 tggctggcgt cggctagggc tttttctgtc tgctctacca attcctttgg ggccttctct
     6001 ttatactcag gattttctat gcgcgttctg agtattgatt cttcaccttg aacttccttt
     6061 agctgattct caagtgcgct tcggtacttt gcaacatcaa tgtctagcca aaccttaatt
     6121 ccctgagcag ggaattcaat actgataaaa ctgttttttt cagtatcagt ttcgtctaca
     6181 ctttttagtc tagctaacct tttcagcaag ttggcattat cctttaaggt taatgaagac
     6241 tccatgaaga aaaggttagg cctcacaaag tcggctaagg ttgaggccgc cctgctccat
     6301 attgtgagtt gaagaatcaa ctgaaaatct tctgcttctt gagcttcaaa ttcacaaatt
     6361 tctggccagg gctggcttat cagcattgag ttctgccagc ccagctcgcg ccagatggtt
     6421 tcggtcagaa acggcgcgaa aggatgggtt aacttaaggg tttgctccag cacatacgcc
     6481 agcagcggcg cgttaagttc aactttgctg gcttctatgt accagtcggc aaaatcatcc
     6541 catacaaaat gataaagttt gtcgtaagcc tcggcgaagc ggaagttggt taggtcctca
     6601 cccagctgtt gtgaaaaaat acttaacttg ttaagtatcc agtgatcagc gatgcttttc
     6661 ggtacagctc cttctctact gtcttcgcca accgcgtctt caacatagcg ggcgatgttc
     6721 caaagcttgt tgcagaagtt gcggttggcg acgattttgg ccaggcccag ccgctggtca
     6781 ttccctgccc cggtgccgct gaccagcgcc agccgcagcg cgtcggtgcc gtatttcgct
     6841 atcatgtcca gaggatcaac gatggtatcc gggtcgcttt tgctcatttt cttgccctct
     6901 tcagtgcgca ccaggccatg cagatagacc gtcctgaacg gtatctgacc cgtcacatag
     6961 gtggtggcca aaatcatgcg cgccacccag aaaaataaga tgtcccaacc ggtctccatc
     7021 acagaggtgg gatgataagt tctataatca acgctcttct ccagcagctg gtcaagcgac
     7081 agcgaatgat ccgcagccag cttcgggtca atcagcgtgc tccaggtcca caaggccgag
     7141 gaaaaccagg tgtccagcgt atccggatcc tgcttccacc cctgcccttc cggcgcctgc
     7201 gtgcctacat aggtttcttc actatcccct accccctcag ccctatcccc ttgtgcctgt
     7261 ctgtaccaga caggcacttg gtgcccccac cagatctggc ggctgatgca ccaatcgcgc
     7321 agattgtcta tccagtgata gtagattttc tcaaaacgct tggggatgat cctgatgtca
     7381 ccatcttcta ccaccgcccg cataacctct ttaaagctgc gcatctggcc cttccaatcc
     7441 acggccggcc ggttgacgtc cacgaaccac tgcagcataa tctgcggctc aataacacct
     7501 ttgcctcggc tgttcagggc gatattgtgg gtgtaatcct cgtctacttt gaccagcagg
     7561 cccttggcct taagcttgtc aacgattttt ggccgtgcct cctctatgtc cattccggaa
     7621 aattcgccgg ctatgggcag caatttacct ttgtaatcaa tgatttgttc ccgctccagc
     7681 ccgtggcgct ccgcgatttc aaagtcggtg cggtcgtgcc acggagtgat ggtcatcacc
     7741 cctgttccaa actccggatt gacggcctcg tccttaacga tagtggcggt cactttaccg
     7801 ttcagccatt cgcattcaaa agtctggccg tcgctgtagt tcttgtagcg cttgtcttcg
     7861 gggtggacca ccacgtactt gtcgccgaac ttggtctccg gccgggcggt gcttatctgg
     7921 aaaggaccgt attgtaaggt gtaaagcggg gccttctctt ctacataatc aacctcgtcg
     7981 tcagagacgg tggtttccag cttgggatcc cagtttacaa tgcgatgacc cctgtaaatc
     8041 aacccgtcct ggtacatttt gttaaagact tcattgaccg agcggttcag cgaatcatct
     8101 aaggtgtaac ggaaccggct ccagtcgcag ctggccccca tggcccgcat ctgtgaaatc
     8161 atggtttcgc ggttaccgcc ggcaaactcc ttgaccttag cgataaaagc ctcgcggccg
     8221 atctgatgct tgtcggtccc ctgctctgcc agctgccgct ccactacggc gttggtggcg
     8281 atagcggcgt ggtcggtacc aggcagccac agcacgtctt tacccagttg gcgggcgtgc
     8341 cggaccataa tgtcttgcag ggtcaaaaac agcgccccgc ccatatgcag cgtagaggtt
     8401 tcgttgggcg gcggcatgct aacagaaaag tgttccttac cactgttggg gtcggcttta
     8461 aaaacaccgc tgtcttccca taatttatag atgtcggctt cgtatcggtt gggctcgtag
     8521 gccttggcta gcttcataaa gtcttagtcg cattatacag aggtggagaa gtaaaatcac
     8581 gtcttccctg ttattcgctt cccttcacgt gaaaaattac actcaacaac aacctttcgc
     8641 gtgaaaaggc tgtctcaaaa actgcctgtc ataaacaggg actgtccgat accggaggtt
     8701 tgtctttacc acccaatctt tgttttcaat ctcaagcata acacgaatag gggagtaggg
     8761 taatagggtt aaagggttat agaaagagtc cttccaccct acacccctac aacccttcaa
     8821 ccctgttttt tacatctgta aactgggatt aatttccagt gaataggggt cggctggcgg
     8881 ccgtttgcgg ctagcctgat agtaagcctg gacggctatc atcgcggcgt tgtcggtgca
     8941 caattttata tcgggataaa ctggggtagg ggacagtttg gcggccgccc ggcgcagtgc
     9001 ctggttggcg gccacaccgc caacaatcac cacacttttg gggtgataaa cttctatcgc
     9061 catcttcagc ccatctgtta gtgatgcgat ggcggtggcc tggaagctgg cggctaaatc
     9121 ggccttttgc ttagccttta atacctttgg taggccggtc gaaggaaaag taaaatcttt
     9181 accggccatc tgttgggccg cccgcagaac ggcagtttta agtccggaaa aactgtagtc
     9241 gtaaggatta tccagcttgg ctataggtaa tttaacagaa gcggggtcgc cctttttggc
     9301 ggcttggctg accgacggcc cgccggggta gggcaggcct aagattttgg cgactttgtc
     9361 aaaggcctcg cccacggcgt catcgcctgt ctgacctaga acccggtaat caaagtggtt
     9421 cttgaacagc accagctggc tgtggccgcc ggaaactatc agcgctaaaa tcgggaattt
     9481 tggcgcagcg gcgggtagcg agtagatagt agcaagggca ggggaaactt ttgtgatgaa
     9541 tgtggcgtaa acgtgggctt ccaagtggtt gacggcgtag agtggcttgt tcttggcgat
     9601 agccagggtg cgcgcggtca ggacgcctat tagcaaactg ccggccaagc cagggccgta
     9661 ggtcacccct atggcgtcaa taccgcccca atctgtttta gcagttttta gcgcctcatc
     9721 tatcaccggg atgattgctt ctatgtggct gcgggcggca atctccggca ccacgccgcc
     9781 atacgcggca tgcaaatcca cgctgctggc cacaacgtta gacagcatct tccgaccgtc
     9841 ttccactacc gctgcggcgg tttcatcaca actggtttct attcccaaaa ttctcatcag
     9901 ttgtacagta tactagagtt atggctgatc tgaaggccgg cgtttaccgc cactacaagg
     9961 gattattggt ccaggtgctt ggcaccgccc ggcacagcga gaccgatgag aaatttgtag
    10021 cgtacgtacc cctgggcgtc aaagcaggcc cgcgcattac tgtccgaccg tacaatatgt
    10081 tttttgagga ggttgagatc gagggtaaaa agcagttgcg ctttgaattt ataggcgaaa
    10141 ctgtcagcga agaaattgcc aaagattatg acccgtattc tcagagaaga gcgacactat
    10201 gaactcacga aaagtcgtta aaagcttaat tcctaagaag ttgtttgaga aagttgagcc
    10261 ggctgggcac ggggtcgaag ctgcgctggt taacgcgaaa aaccgctggc cggcgcgggg
    10321 gttaaatgtg attggcgtaa ccggcaccga cggcaagact acaacctgta tgctaatcgc
    10381 taaaatgctc agaaacagtg gtcaaaaagt agcaatgatt acaacggtgg ccgttgatta
    10441 tgctgacggt cagggcgagc gtcctagccc cacgcacctg actaccgcca gcgccggcca
    10501 gctgcaagcc atgttcaaaa aaatcaaagc taaccagccg gactgggtag tgctggaagt
    10561 ttccagccat gccttggcgc agtaccgggt tttgggcgtg ccgttttctg ttgccgtcat
    10621 gaccaacctg acccacgagc acctggatta ccacaaaact tttgcgcgct accgcgacgc
    10681 caaagtcagg ctcttcaaac tggccgcccg caaccaccgc ggcctcagga ccggtgttat
    10741 aaacgccgat gatccgtcag ccgaatattt catccgtgcc gtaccccgat ccgtcaccta
    10801 cggaatcaaa aatggagcgc tgcaggcgaa aatccttaag acaaccgccg ccggtagtga
    10861 attccaagtc ggcggcctgg caatcaacct caacttaccg ggtgaattta acatttataa
    10921 cgccttggca gccataggag caggtcaagt catcggacta agtgatgaac agatagggca
    10981 ggggatcgcc gctttgaaag ccgtaccagg ccgtatgatg cgcctagaag ccggccaacg
    11041 gcttgaagtg ctgattgatt atgccgtgac gcccgcggcg ctggaaaacg tcctaaaagc
    11101 cgccagaaag acgaccaaag gcaaagtaat gctggtcttt ggcgccaccg gcgaccgcga
    11161 caaagccaaa cggccggtaa tgggcgaagt ggccgccaaa tttgccgaca ggatattcct
    11221 gacggatgat gaaacctatc gcgaagaccc ggcggccatc cgccaagcgg ttatgacggg
    11281 gattgccaaa gccggcggcc aagccaaaac aacagaggtg aacgagcgcg cagcggccat
    11341 caaacaagct attgccaaag ccaaagccgg cgacacggtt ttaataaccg gcttgggcca
    11401 ccaaaaagac cgaaacatgg gcggcaaact catcccttgg agcgacagcg aaattgccaa
    11461 acagttatta aaccgtcagc accgttgaca aaattaagct taagtggtat aatggcgcac
    11521 aattgatggc gactatccct tcatggcaag taaagaacta caggccttta ttatgcaagc
    11581 acatactgta gtcgctttca taaacactag cgtcataatg acccggctca taagctgagc
    11641 ctatttcgtt ttgcaaccat tgttccgaac cagtaatcgc ttgtt
//
LOCUS       DASPTF010000022         4522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1609803, whole genome shotgun sequence.
ACCESSION   DASPTF010000022 DASPTF010000000
VERSION     DASPTF010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609803"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /gene="pilM"
                     /locus_tag="VEH48_00670"
     CDS             <1..345
                     /gene="pilM"
                     /locus_tag="VEH48_00670"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HXY17919.1"
                     /translation="AEQFVYKFGLGKDKLEGQVYNAIIGTVDGLINEIEKSISFFQGR
                     YPTLKIERIVVTGGASSLPEFPLYIANKFGLNVEIGNAWRNVSFPPARQNELLTVSNH
                     FAVAVGLAERNG"
     gene            342..1037
                     /locus_tag="VEH48_00675"
     CDS             342..1037
                     /locus_tag="VEH48_00675"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXY17920.1"
                     /translation="MSGVQFNLLPDVKLAYVKAQRSRRLVTVVAVLLSSVAIAILLIM
                     VFTVEVVQRKQLNDAAAAVTTANKQLSGIAGLDQVMTVQNQLYALATLHQQKHISSRL
                     MLPYLPNITPSNVQISQVSLDLTKNTLSISGTAASQLAVNTFVDTLEFAQFKVGSQSA
                     RAAFTSVVESTFSLTNNGTTYTITANVDPGLFANTGQTPQIILNNQITTRSVLEDPSN
                     FLFTGTNSKQGSQ"
     gene            1037..1723
                     /locus_tag="VEH48_00680"
     CDS             1037..1723
                     /locus_tag="VEH48_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17921.1"
                     /translation="MVKLPETSKRARIDKTQSTMLAIISIAAVITIFCLMSAKALLSQ
                     AAYQRKVVKANQAAVKQLQANVTAAKALVTQYSQVFEGTNPSNIIGGQNTTSPDAVPP
                     NGDNARIVLDALPSKYDFPALISSVSNILTDDKITSPSVTGTDESATIDNNAATNPQP
                     QQIQLTVTGTGTYDNVLAFVQDLERSIRPFDVVSLQLAGPQDSLTFTLTVNTYFQPAK
                     SLNVVTQEVK"
     gene            1727..1990
                     /locus_tag="VEH48_00685"
     CDS             1727..1990
                     /locus_tag="VEH48_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17922.1"
                     /translation="MKRGDIVKVVLVAGMSAVISFIIAAAIFNSPAQHNRRVPVVQPI
                     TSTFPDITNDPNYNTIFNSKALDPTQPVQIGNSQNNSPFIGSQ"
     gene            2012..3820
                     /locus_tag="VEH48_00690"
     CDS             2012..3820
                     /locus_tag="VEH48_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXY17923.1"
                     /translation="MNILSPTAQKEVEEVLVADGLVTETQLEQIQDKAAQNHQPVFTT
                     LLSDGYINDEQLTKALAVVSKVPYVNLSKAHIDSKMLALLPRDVAEQYMAVPIGEMQH
                     RLVVAMLDADNVQAVDFLSNKINRPLKVYAASESGIRRVLKQYDQSINTGDTDIDFSA
                     LGTPGAVAEGQGAGITPVGTAERKVQTIVQDSPVSKALSAIMEYAAKNRASDIHMESL
                     EKEIRVRCRIDGVLREVMTLPKNAEAPIVSRVKILSNLKIDEHRIPQDGQFSVIADKR
                     PIDIRVAISPVVWGEQVVLRLLDKTGTSLKLEDMGYTGRALREIRKGLTRPHGMILTS
                     GPTGSGKSTSMYAMIQEIKNDTINIVTLEDPVEYKIEGVNQIQVNPDVGLTFANGLRS
                     ILRQDPDVVMVGEIRDKETAALAVQAALTGHLVFSTLHTNSAAGILPRLLDMSIEPFL
                     IASTVHTVLGQRLVRRVIAGGESYQSTKPETEAIHQTLGALLPKTKTDEEKAAKDLGY
                     SHLPLSNQNAYTLVKGKETPENPTGYEGRMGLYEVFEVTEAIQDLIIKRAPSSVIQRA
                     AQAQGMITMRQDGYLKALAGITTLAEVNRVAAMGDS"
     gene            3838..>4522
                     /locus_tag="VEH48_00695"
     CDS             3838..>4522
                     /locus_tag="VEH48_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXY17924.1"
                     /translation="MQQSPKIEVLLEEVVKRKASDLHLQVGLSPILRIDGALVSIAGQ
                     AKLSEETVENLVFSLLDEDQKQILLRDKEFDFSFAFGDLGRFRVNAFHERGNLAAAMR
                     LIPNQIRDIEQLGLPPVLNKFTGYPRGLVLVTGPTGSGKSTTLAALVNKINEERAAHI
                     ITVEDPIEYTHTSKKSVVVQREVHYDTYSFSTALRSVLREDPDVVLIGEMRDLETIAA
                     AITIAETGHL"
BASE COUNT         1095 a         1337 c         1215 g          875 t
ORIGIN      
        1 gccgagcagt tcgtttataa attcggcctg ggcaaagata agctggaagg ccaggtctac
       61 aatgccatta tcggcacggt ggacggtttg ataaacgaga ttgagaaatc cataagcttt
      121 ttccagggcc ggtatccgac gttgaaaatt gagcggatcg tagttaccgg cggggcgtca
      181 agcttgccgg agttcccgct ttatatcgcc aataaattcg gcctgaacgt agagatcggc
      241 aacgcctggc gcaacgtcag cttcccgccc gcgcgccaga atgaactgct gacggtttcc
      301 aaccactttg cggtggctgt cggcctagcg gagcgcaacg gatgagtgga gttcagttca
      361 acctgctgcc cgatgtcaag ctggcctatg tcaaggccca gcgcagccgc cgtttggtga
      421 ccgtcgtagc ggtcctgcta agcagtgtgg ccatcgctat tttgctgatc atggtcttta
      481 ccgttgaggt cgtacaaaga aaacagctta acgacgccgc cgcggcggtt acgaccgcca
      541 acaaacagct atcgggcatc gccggcttag accaggtgat gacagtgcag aaccagcttt
      601 acgcgctggc aacgctgcac cagcaaaagc acatcagctc gcgactgatg cttccttatc
      661 tgccgaacat aactcccagc aacgtccaga tcagccaagt cagcctggat ctgactaaaa
      721 ataccttgtc tatttccggc accgctgcca gccagctggc ggtcaacacc tttgtggata
      781 ccctggaatt cgcccagttc aaagtcggtt cgcaaagcgc cagggcggct tttacgtcgg
      841 tggtggagag taccttttcg ctgaccaaca acggcaccac ctataccatc accgccaacg
      901 ttgacccggg gctgtttgcc aataccggcc agacgccgca gataatcctg aataaccaga
      961 ttaccacccg ttcggtgctg gaagacccca gtaatttcct gttcaccgga actaatagca
     1021 aacaggggag ccaataatgg tcaaactgcc ggaaaccagc aagcgcgccc ggattgacaa
     1081 gacccagtcg acgatgctgg ccatcatcag tatcgccgcc gtgataacca ttttctgcct
     1141 gatgagcgcc aaagccctgc tatcccaggc cgcctaccag cgtaaagtgg tcaaggccaa
     1201 ccaggccgcg gtcaagcagc tgcaggctaa cgttacggcg gccaaagcgc tggtgacaca
     1261 gtacagccag gtatttgaag gcactaaccc cagtaatata attggcggtc aaaataccac
     1321 cagtcccgac gccgtaccgc ctaacggcga taacgcccgt atcgtgctgg acgccctgcc
     1381 cagcaaatac gactttccgg cgttaatcag cagtgtcagt aacattttga ctgacgacaa
     1441 gatcaccagt ccatcggtta ccggcactga cgagtcagcc accattgaca acaacgccgc
     1501 caccaatcct cagccccagc agatacagct cacagtcacc ggcaccggca cttacgataa
     1561 tgtcctggct ttcgtccagg acctggagcg ctccatccgg ccgtttgacg tcgtcagcct
     1621 gcagctggcc ggaccgcagg actcactgac attcacgctg acggtcaaca cctatttcca
     1681 gccggccaaa tcgctgaacg ttgttactca ggaggttaaa tagactatga aacgcggtga
     1741 tattgtcaag gtggtgctgg tagccggtat gtcggcggtt atttcgttta ttatcgccgc
     1801 tgcgatcttt aattcgccgg cgcagcacaa ccggcgggtg ccggtagtgc agcccattac
     1861 cagcactttt ccggacatta ccaacgaccc caattacaac acaatcttca attccaaagc
     1921 ccttgatcca actcagccgg ttcagatagg taactcgcag aataattcgc cgttcattgg
     1981 ctcacagtaa gatgatggca ggcaaacact catgaacatc ctatcgccga cggcccagaa
     2041 agaagttgag gaggtgctgg tagctgatgg gctggtcacc gaaacccaac tggaacagat
     2101 ccaggacaaa gccgcgcaga accatcagcc ggtgttcacg accctgctca gcgacggcta
     2161 catcaacgat gagcagctga caaaggccct ggcggtggtc agcaaggtgc cttatgtgaa
     2221 tttgtccaaa gcccatattg attccaagat gctggccctg ctgccgcgcg acgtcgccga
     2281 gcagtacatg gccgtaccta tcggtgagat gcagcaccgg ctggtggtag ccatgctgga
     2341 cgccgataac gtccaggccg tggatttttt aagcaacaaa atcaaccggc cgctcaaagt
     2401 ttatgccgcc agtgaatccg gcatcaggcg ggtacttaaa caatacgacc aaagcatcaa
     2461 caccggggat accgacatag acttcagcgc tttggggact ccgggcgcgg tcgccgaggg
     2521 gcagggcgcc ggtatcacgc cggtgggcac agccgagcgc aaggtccaga ccatcgtcca
     2581 ggattcaccg gtcagcaagg ccttaagcgc catcatggag tacgccgcca agaaccgcgc
     2641 ctccgacatc catatggaat cgctagaaaa ggaaatccgc gtccgctgcc gtatagacgg
     2701 cgtgctacgc gaagtcatga ctctgcccaa gaacgccgaa gcgccgattg tcagccgggt
     2761 gaagatttta tccaacctga aaattgatga gcaccgtatc cctcaggacg ggcagttcag
     2821 tgtcatcgcc gataagcggc cgatagatat ccgcgtggcc atcagcccgg tggtgtgggg
     2881 cgagcaggtt gtgctgcgcc tgctggacaa gaccggtacc agcctgaaat tggaagatat
     2941 gggctacacc ggccgggcac tgcgtgagat ccgcaagggc ctgacccggc cgcacggcat
     3001 gatactgacc tccggcccga ccggttccgg caaatccacc agcatgtacg ccatgatcca
     3061 ggaaatcaag aacgacacca tcaacatcgt tacactggaa gatccggtgg aatataaaat
     3121 agaaggcgtc aaccagatcc aggtcaaccc tgacgtcggc ctgacttttg ccaacggcct
     3181 gcgctctatc ttacgccagg acccggacgt ggtgatggtc ggcgagatcc gcgacaaaga
     3241 gaccgccgcg ctggcagtgc aggcggcgct gaccggacac ctggtttttt ccacgctgca
     3301 caccaacagc gccgccggca tcctgccgcg gcttctggac atgagcattg aaccgttcct
     3361 gattgccagc accgtgcata cggtacttgg ccagcggctg gtgcggcggg taatcgctgg
     3421 cggcgagagt taccaatcca ccaaaccgga gaccgaagcc atccaccaga ccttgggcgc
     3481 attgctgccc aagaccaaga ccgacgaaga aaaagccgcc aaagatctgg gctactcgca
     3541 cttgcccctg tccaaccaaa acgcttatac tttggtcaag gggaaagaaa cgcccgaaaa
     3601 tcctaccggc tacgaggggc gcatgggact ttacgaagtc tttgaagtca ctgaggccat
     3661 ccaggacctg ataatcaagc gtgcccccag ctcggtcatc caaagggctg cccaggccca
     3721 ggggatgatc accatgcgcc aggacggtta tttaaaagcc ttggccggca tcactacttt
     3781 agccgaagtc aaccgcgtag cggccatggg tgatagttag gggaatgggc taatattatg
     3841 cagcaatcgc cgaaaataga agtcttactg gaagaagtcg tcaagcgtaa agcttccgac
     3901 ctgcatctgc aagtcggcct gtcgccgata ctgcgcatcg acggcgcgct cgtcagcatc
     3961 gccggccagg ccaagctgtc cgaagaaacg gtggaaaatc tggttttcag cctgctggac
     4021 gaggaccaaa aacagatcct gctacgcgat aaagagtttg attttagctt tgcctttggc
     4081 gatctgggtc gcttccgggt caacgctttc catgagcgcg gcaacctggc cgccgccatg
     4141 cgcctgattc ctaatcagat ccgcgatatc gaacagctgg gattgccgcc ggtactcaat
     4201 aaattcaccg gctatccgcg cggcctggta ctggtcaccg gcccgaccgg ttccggtaaa
     4261 tccactacac tggccgcctt ggtcaacaag attaatgaag agcgggcggc gcacatcatc
     4321 accgtggagg acccgattga gtacacccac acttccaaaa aatcggtggt tgttcagcgc
     4381 gaagtgcatt acgacactta cagtttctct acggcgctgc gttcagtact gcgcgaagac
     4441 cccgacgtgg tactgatcgg tgaaatgcgt gacttggaga ccatagccgc ggccatcacc
     4501 atcgccgaga ccggccacct gg
//
LOCUS       DASPTF010000023         9922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1468120, whole genome shotgun sequence.
ACCESSION   DASPTF010000023 DASPTF010000000
VERSION     DASPTF010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9922)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9922
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468120"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="recO"
                     /locus_tag="VEH48_00700"
     CDS             complement(<1..681)
                     /gene="recO"
                     /locus_tag="VEH48_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXY17925.1"
                     /translation="MKQFTTQGIVLTRTDFGEADRIITFLTPDHGKVRAIAKAVRKSQ
                     SKLAGGIELFSVSNLTFVAGRGELHTLISSRLDKHYAGIVKDLGRTNAAYELIKLVNK
                     ATEEKNESGYFDVLHQAFAALDDSAVDPQITELWCRLRVLDLAGHAPNLQNGPDGQKL
                     PPAKTYNFDFEHMCFAPADAGQGSYSPQDIKFLRLGSAAAHPQLLQKVQGVDKVIPKT
                     SMLVDSLLD"
     gene            complement(678..1061)
                     /locus_tag="VEH48_00705"
     CDS             complement(678..1061)
                     /locus_tag="VEH48_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXY17926.1"
                     /translation="MAKILLIESDKLLADNLRDFLEAAGHSSHWQVEPQAALDAVDSF
                     KPNLIIMDLLLASRSGTEFLYELRSYPDWESLPVIIYSSLADGELGQWLPQGLEHLDI
                     AAYHPKTAAGLGELGRSINCLLQPA"
     gene            complement(1065..1811)
                     /locus_tag="VEH48_00710"
     CDS             complement(1065..1811)
                     /locus_tag="VEH48_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17927.1"
                     /translation="MKRFISDRGHEQLMQQRALLTALSDDLALPLVQIRSALEIMEGG
                     DFAKKIVRSQTANMGLSAQAGLQLIEAYRLALQAQDEQSLPLVPVSVAGVLQDVAHNL
                     SPYAKQYDTDLQVDVVGRLTPVLAHQPSLMAALEVLGASMVRAQAAQSRQKRYRLLLG
                     AHRTPDNAIAAGVFSSMDGLSDRTLRTARSLAGRARQPLLALPAGAASGILVADMLCA
                     AMWQPLRSAAHRNLHGLATAVPISKQLQFV"
     gene            complement(1904..2581)
                     /locus_tag="VEH48_00715"
     CDS             complement(1904..2581)
                     /locus_tag="VEH48_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17928.1"
                     /translation="MKRSESGSAGSTLVTVVLLVVAVGALAFGFWAYSGRQDYKNNAD
                     QKTAAAVAAAEAAQKTQLQAQFNQQLKSPYKTFVGSITYGSITFQYPNAWSSYVDTTN
                     SSEPINGYYNPGDVPGTGSGSAFALRVELTGNGYNSVIQNFNSQVQSGTVTATAYVPP
                     KMKSVANVQPGTLLTGAVSNSNGSPLNGAMLVIPVRDKTLQIYTLSNDYLSDFNNIVL
                     ASLTFVP"
     gene            complement(2677..3567)
                     /locus_tag="VEH48_00720"
     CDS             complement(2677..3567)
                     /locus_tag="VEH48_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17929.1"
                     /translation="MWPFNIRKKRREARERQASIKREAFRQRLLQLIEKVEARNITQD
                     EIREFRSISDNVLAKGGWLRWSDACDIIYAIERMDRDGKSVQHALDWLKDAENLDQQV
                     FRLADLLSKYNKDVDGLNAPLLEKEFGHDRKKLLTRLKRLTKKRYQELLGERTKGAKQ
                     LEDLGIFIRETHSRRNMNGRWEPNRLKAVSVEPLPYPEDWNELVALFYPTPTPDQFID
                     LAMNSGNVLLLAEEAGRMANAKEAFVNAQIVLAYCRAEANYRIHVGDILVTRMAEIVN
                     AYRESQSATAAGKGVADVAE"
     gene            complement(3650..4156)
                     /locus_tag="VEH48_00725"
     CDS             complement(3650..4156)
                     /locus_tag="VEH48_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXY17930.1"
                     /translation="MRWANDFFWVIWFGALLLILAYGTVLLFGAPYFPSLKPHLKAAL
                     KLLDLKKGQVVYDLGCGDGRFLKAAARSGYSAVGYELNPFVFAYAWLTTRRYSRRVRV
                     RYGNFWHADISQADAVFVFLLDRWMPRLDKKLKKEGKPGLKLASHTFKIPGKKPAAQN
                     YGVFLYEY"
     gene            complement(4257..4457)
                     /gene="xseB"
                     /locus_tag="VEH48_00730"
     CDS             complement(4257..4457)
                     /gene="xseB"
                     /locus_tag="VEH48_00730"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXY17931.1"
                     /translation="MAKKSTGGKSYAQLSAELGEVMDWFEGGEVDLDEALAKYQQATK
                     LIEQMENYLKTAANKIKKINKV"
     gene            complement(4450..5568)
                     /gene="xseA"
                     /locus_tag="VEH48_00735"
     CDS             complement(4450..5568)
                     /gene="xseA"
                     /locus_tag="VEH48_00735"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXY17932.1"
                     /translation="MQDVVLSPSDFVALLNQTLEFAYPVVTIEGELSGFRISKNRWVY
                     FDLQDEQATVKFFGTVYALPGPLQDGLKVRVVGAPRFHPRFGFSVNIQSITPTGEGSI
                     KKAFQLLRAKLEREGLFAPERKRPLPPVPGRVGLVTAANSAAYHDFIKILNERWGGVE
                     ILLADVYVQGDQAPAQITGAIEILNKSSILPEVIVITRGGGGADDLAAFNDERVVRAV
                     AASRAPTLVAVGHEVDLSLAELAADVRASTPSSAAQILVPDRTAELASLDVSCQSLKR
                     NLATVYDRAVMELVDSRDTLTVQMTSRLNTTAERLHTLSRLVALLDPGAALRRGYAII
                     RKGGKNIRSVRQVKIGDRLSLQLQDGTIGANTTEVING"
     gene            complement(5573..6622)
                     /locus_tag="VEH48_00740"
     CDS             complement(5573..6622)
                     /locus_tag="VEH48_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXY17933.1"
                     /translation="MSESNPLKQSRVKLFLTIATIIALAGLIYGLRKDIGGVIDNLRK
                     VNTLALLLMIPIMALSNDADARFFRNVFATLGQKVRYWPMFRMNLELNFVNHILPSGG
                     ISGISYFNVRMRGEGVSGAKSTLAQVVKLFLLYASFQPLLVLGVFLLAVRGHVNGLIL
                     VVASSLITLLIVGTFIGLYIIESRQRINSFLTFMTKAINRLIHFFRPHKPETIGIKNA
                     QKTFNELHENYEHIKNNWRELKKPFFYMMVTNASEVAAIYVVYIAFGRLVNVGAVILA
                     YAVANFAGLISVLPAGIGIYEGLMTAVLVATGIPAELSIPVTIMYRVVNMALQLTPGY
                     YFYQQAVKQGLTSKI"
     gene            6768..7964
                     /locus_tag="VEH48_00745"
     CDS             6768..7964
                     /locus_tag="VEH48_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17934.1"
                     /translation="MAGFEAKSLCILGRQPALGLAELESLYGPEHIQPLSGTALLDLE
                     AGDINFKRLGGCIKVAKVLASLPGAGWNDIKKYLLENVPKHLAHAPAGKFTLGLSFYG
                     LAPPVTEINKLALDIKRASGRPLRVVPNKSATLNSAQVLHNKLTSKGAWELLLVKNGQ
                     RTILAQTFFVQDIEAYGARDQARPKRDARVGMLPPKLAQIIINLAAGPLDHEIENKTK
                     IRVLDPFCGTGVVLQEALLMGYSVLGSDIEPRLVGYSEENLKWLVARQPKIEGNVALE
                     AADATSATWPRFTTVGSEVYLGRPLAKLPEPAKLKEIISDVDTIIKKFLKNLAPQLKS
                     GRPVCLALPAWRQPNGQLIKLPIIDQLTDMGYNYMDLKHVGRQDLVYFREDQVVARQL
                     IRIARK"
     gene            7965..8237
                     /locus_tag="VEH48_00750"
     CDS             7965..8237
                     /locus_tag="VEH48_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXY17935.1"
                     /translation="MSKVKAGGSSSNVHDSPGQRLGLKVFGGAKVKAGDVLVRQVGMT
                     KRSGAGTFMSRDFTIHAAKDGTVKFSKRRFKRFSGRTIPRTEVSVE"
     gene            complement(8239..8661)
                     /locus_tag="VEH48_00755"
     CDS             complement(8239..8661)
                     /locus_tag="VEH48_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXY17936.1"
                     /translation="MSLENSDSGQSQPVPAAKPKKILLVEDDNNLADVYLTRLKAEGF
                     DVRRVADGEEALSAAIEYKPDLVVLDIMMPKVGGFDVLDILRNTPETTNLKIIMVTAR
                     GQDSDRQRAENLGVDDYLVKSQVVIADIITRIKQQLNA"
     gene            complement(8695..>9922)
                     /locus_tag="VEH48_00760"
     CDS             complement(8695..>9922)
                     /locus_tag="VEH48_00760"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXY17937.1"
                     /translation="SRIAETGRGFDVHIVEIGGTVGDIESQSFLEAIREMGQRLGAEN
                     ALFVHVVYIPYLGTSEEFKTKPAQNSVRELRGLGVVPDVLIARSEQKPPKGILNKLSM
                     FTGVDKEAIALLPNAQTIYQVPLSLEEAKIAEVITGRLGLRRLQKPDLAAWRRVVKSA
                     LSKYRKTVRIGIIAKYVDNQDTYICVFEALRAAAWANEVNVDIVWVNAEALERDKKER
                     EILPTLDGIIVPGGFGERGLEGKIIAARYSLDNKVPYLGLCLGLQMAVIAAARNAGLN
                     DATTVELNPKSPRKVIDYMADQHDKKNTGGTMRLGDYPTVLAAGSKARKAYGQEKITE
                     RHRHRCECSNTYRDRYQNWGIVASGLSPDGHLVEVIEARDHPYFLATQAHPEFRSRPN
                     RPHPLFLGLVRACLKR"
BASE COUNT         2073 a         2751 c         2824 g         2274 t
ORIGIN      
        1 atccagtagg ctgtcgacca gcatgcttgt tttaggaatg actttatcta ccccttgcac
       61 tttttgtaat aactggggat gggcggcggc ggagccgaga cgcaggaatt ttatatcttg
      121 cggactgtag ctgccctggc ccgcgtccgc cggcgcaaag cacatgtgct caaaatcgaa
      181 attgtaggtt ttggccggcg gcagtttttg gccatcgggg ccgttttgca aatttggagc
      241 gtggccggct agatctaaca ctcgcaggcg gcaccacagt tcagtgattt gcgggtcaac
      301 ggccgaatcg tctagcgcgg caaaggcctg gtgcaatacg tcaaagtatc cgctttcgtt
      361 tttctcttcg gtggctttgt tgaccagttt gataagttcg taggcggcgt tggtgcggcc
      421 gaggtctttg acgataccgg cgtagtgttt gtccaggcgg ctggagatga gggtgtggag
      481 ctcgccgcgg ccggcgacga aagtcaagtt gctgacgctg aacagttcaa tgccgccggc
      541 cagtttgctt tggcttttgc ggacggcttt ggcgatggcc cggaccttgc cgtgatccgg
      601 tgtcaggaaa gttataatcc ggtcggcctc gccgaagtcg gtgcgcgtta gcacgatacc
      661 ctgggttgtg aattgtttca tgcgggttgg aggaggcagt tgatgctgcg gccaagttcg
      721 cccaggccgg cggcggtttt ggggtgatag gcggcgatat ctaaatgttc caaaccttgc
      781 ggcagccatt ggccaagttc cccgtcggcc aagctgctgt aaattatcac cggtaaactt
      841 tcccagtcgg ggtagctgcg caactcataa aggaactctg tgccgctgcg gctggccagc
      901 agcagatcca tgataatcag gttgggcttg aaagaatcta ccgcgtccaa ggcggcctgc
      961 ggctccacct gccagtgcga gctatggccg gccgcctcta ggaagtcacg taaattgtcc
     1021 gctagcagtt tgtctgattc tatcagcaga atcttggcca tggcttaaac aaattgcagt
     1081 tgcttgctga tgggcacggc ggtagccagg ccgtgcaggt tgcggtgggc ggctgagcgc
     1141 agtggctgcc acatggcggc gcagagcata tcggccacta aaatgccgct ggcggcgccg
     1201 gccggcagcg ccaacagcgg ctgccgggcg cgcccggcca gcgacctggc cgtacgcaaa
     1261 gtgcggtcgc taagtccatc catactgcta aagacaccgg cggcaatggc gttatcaggc
     1321 gtacggtgcg cgcccagaag cagccggtag cgcttttggc ggctttgggc ggcctgggcg
     1381 cgcaccatgc tggcgcccag tacttccagc gccgccatca ggctgggctg gtgcgccagt
     1441 accggcgtca gccggcccac gacgtctact tgcaaatcgg tatcgtattg cttggcataa
     1501 ggcgataaat tgtgggctac gtcttgcagc acgccggcca ccgataccgg taccagcggc
     1561 agtgattgct catcctgggc ctgcagcgcc agccggtaag cctcaatcaa ctgcaggccg
     1621 gcctgggcac tcaagcccat gttggccgtc tggctgcgga caattttttt ggcaaagtcc
     1681 ccgccctcca taatttccag ggccgacctt atctgcacca gcggcaaggc caaatcatcg
     1741 ctgagcgccg tcaaaagcgc ccgctgctgc atcagttgtt catgacccct atcgctgata
     1801 aaacgtttca caaaccctct ttaaagtccg gtcttaccct tcttagctct tagtataaca
     1861 ccctttaaat tgttcaactt ttagacagca tttattacaa tgcctacggc acaaatgtca
     1921 ggctggccag cacaatgttg ttgaaatcac ttagataatc gttagataaa gtgtaaatct
     1981 gaagggtttt gtcgcgcacc ggtatgacca gcatcgcgcc gttaagcggt gaaccgttgc
     2041 tattgctaac ggcacccgtt agcaacgtgc cgggctggac gttggccacg cttttcattt
     2101 tgggcggtac gtaggccgtg gcggtgacgg tgccgctttg gacctgcgag ttgaagttct
     2161 gaatcacgct gttgtagccg ttgccggtca gctcaacccg caacgcgaaa gcgctaccgc
     2221 tgcctgtccc cggcacatcg ccggggttat agtaaccgtt aatcggttcg ctggagttag
     2281 tcgtgtccac gtacgagctc caagcgttgg ggtactgaaa agtaatggag ccataggtga
     2341 tactgccgac gaaagttttg taagggcttt tgagctgctg gttaaattgg gcctgaagct
     2401 gggtcttttg ggcggcttcg gcggcggcga cggcggcggc agtcttttgg tcggcattgt
     2461 ttttataatc ctggcgcccg gaataggccc aaaagccaaa ggccaaggcg ccaacagcca
     2521 cgaccagcag aacaacggtg actaaggtcg agccggccga cccactctcg ctacgtttca
     2581 tactctgctt atgttaataa atttctaccc actaagcaat cggtcctggt tataatttgc
     2641 tgttgatgtg gggcggtcaa gaccgcccca catcagttac tcggcaacgt cagcgacgcc
     2701 tttaccagcg gcagttgcgc tctggctttc gcggtaggcg ttgacgatct cggccatcct
     2761 ggtgaccaga atgtcgccta cgtgaatgcg atagttcgct tccgcgcggc agtaggccag
     2821 cacgatctgg gcgttgacga acgcttcctt ggcattcgcc atgcggccgg cctcctccgc
     2881 caggagaagc acattgccgc tgttcatcgc cagatcgatg aactggtcgg gtgtgggcgt
     2941 tgggtagaac aacgccacga gttcgttcca gtcctcgggg tacggcagag gttccacgct
     3001 aaccgccttg agccggtttg gctcccagcg gccgttcatg ttcctcctgc tgtgcgtctc
     3061 acggatgaag atgccgaggt cctcaagctg cttggcaccc ttcgttcgct ccccaaggag
     3121 ctcctggtag cgcttcttgg tgaggcgctt caggcgggtg agcagcttct tgcgatcgtg
     3181 gccgaactcc ttctccagca gcggcgcgtt gagcccgtcc acatccttgt tgtacttgct
     3241 cagcaggtct gccaaacgga agacctgctg gtcgaggttc tcggcgtctt tgagccagtc
     3301 gagagcatgc tggacgctct tgccatccct gtccatccgc tcgattgcgt agatgatgtc
     3361 gcaagcgtcg ctccaccgca gccatccacc tttggccagc acgttatcgc tgatgctgcg
     3421 gaactcacgg atctcgtcct gcgtgatatt gcgcgcctcc actttctcga taagctggag
     3481 gaggcgctgt ctgaaagctt cacgcttgat gcttgcctgg cgctcgcgcg cttcacgccg
     3541 tttcttgcgg atattgaagg gccacatcag cccgtcacct cccggttgtc gaataacgtc
     3601 ttgggtcttc ccaaagatta cattattata atattattgt tcttatttat caatactcat
     3661 acaggaagac gccgtagttt tgggcggccg gttttttacc ggggattttg aaagtgtggc
     3721 tggccagctt taggcccggc ttgccttctt ttttaagctt tttgtccagc cggggcatcc
     3781 atctgtccag caaaaacaca aagacggcgt cggcctggga gatatcagcg tgccaaaagt
     3841 tgccgtagcg gacgcgtacc cggcggctgt agcggcgggt ggtcagccag gcgtaagcga
     3901 acacgaaagg gtttagctca taacccacgg cgctgtaacc gctccgtgcc gcggctttta
     3961 agaaccggcc gtcgccgcat cccaagtcat aaaccacctg gccttttttg aggtccaaaa
     4021 gtttcagcgc cgccttgaga tgcggcttga ggctggggaa atagggcgcg ccgaacagca
     4081 ggacagtacc ataggccaga atcagcagca gcgcgccgaa ccagatgacc cagaaaaaat
     4141 cgttagccca gcgcatgcgg cttacgcctt atgaggacaa accattggtt tttcctcatc
     4201 gcgccgggcg cggagtgaat ccgacgctcg ggctgctctt tttcctacca tcatcttcag
     4261 accttattga ttttcttgat tttgttggcg gcggttttga gatagttttc catctgctca
     4321 atcaacttgg tagcctgttg gtatttggcc agggcttcgt ccagatccac ttccccgccc
     4381 tcaaaccaat ccataacctc gcccagctcg gccgatagct gggcgtagga cttgccgccg
     4441 gttgattttt tagccattga taacctcggt agtgttcgcc ccaattgtac catcttgcag
     4501 ctgcaggctc aggcggtcgc cgattttgac ctggcggact gagcggatat ttttgccgcc
     4561 tttgcggatg atggcgtagc cgcgccggag ggcggccccg ggatcaagca atgcgaccaa
     4621 acggctgagg gtatgcagcc gctcggcggt ggtgttcagt ctggaggtca tttgcacggt
     4681 caatgtgtct cggctgtcga ccagctccat cacagccctg tcataaacag tggccaggtt
     4741 acgcttgagg ctttgacaag atacatccaa gctggccagt tcggccgtgc ggtcgggcac
     4801 aaggatctgg gcggcgctgc tgggcgtgct ggctcggaca tcggcggcca gttcggctaa
     4861 agataaatcc acctcgtgcc cgaccgccac cagcgtcggc gcccggctgg cggctaccgc
     4921 ccgcacaacc cgttcgtcat tgaaagccgc caggtcatcg gcaccgccgc cgccacgggt
     4981 tataacaatc acttccggca aaatacttga cttgttaagt atttctatgg cccctgttat
     5041 ttgcgccggc gcctggtcgc cctgcacgta gacatcagct agcaatatct ccacgccgcc
     5101 ccagcgctcg ttcaagattt ttataaagtc gtggtaagcg gcggaattgg cggccgtcac
     5161 cagccctacc ctgcccggca ccggcggcag tggacgtttg cgctccggtg caaacaggcc
     5221 ctctctttcc agcttggctc gcaagagctg aaaggctttt ttgattgaac cctcaccggt
     5281 cggtgtgatg ctctggatgt taacgctgaa gccgaagcgc gggtgaaagc gcggcgcgcc
     5341 gaccacccgg accttcaatc cgtcctgcag cggcccgggc aaagcgtaaa ccgtgccgaa
     5401 aaacttgacc gtggcctgtt cgtcctgcaa gtcaaagtag acccagcggt ttttactgat
     5461 acggaagccg ctaagctccc cttctatggt cactaccggg taagcgaatt ccagagtctg
     5521 gttcaaaaga gcgacgaaat cggacggcga aagcactacg tcttgcatgg cgctaaatct
     5581 tactggtaag gccctgcttg acggcctgtt gatagaaata gtagcccggc gtcagctgca
     5641 gcgccatatt gactacgcgg tacatgatgg tgaccgggat actgagttcg gccgggatgc
     5701 cggtggccac tagcacggcg gtcatcagac cttcgtaaat accgatgccg gccggcagta
     5761 cggaaatgag tccggcaaaa ttggccacgg cgtaagctaa aatcaccgcg ccgacgttaa
     5821 ccaggcggcc gaaagcgatg taaaccacgt agattgccgc gacctcggaa gcgttagtta
     5881 ccatcatgta gaaaaacggt tttttgagtt cgcgccagtt gttttttatg tgctcgtaat
     5941 tttcgtgcag ttcgttaaag gtcttttggg cgttctttat gccgatagtc tcgggcttgt
     6001 gcggccggaa aaaatgtatc agccggttga tggctttggt catgaacgtc aggaacgagt
     6061 tgatgcgctg gcggctttca atgatataaa gaccgatgaa agtgccgact atcagcaaag
     6121 taatgagtga actggcgacg accaggatca agccattaac gtggccgcgt acggccagca
     6181 aaaaaactcc aagaaccagg agcggctgga aactggcgta cagcaagaac agtttaacaa
     6241 cctgggccaa tgtggattta gcgccgctga cgccttcgcc gcgcatccgg acattaaaat
     6301 agctgatgcc gctgataccg ccgctgggca ggatatggtt aacaaagttt agctccaggt
     6361 tcatgcggaa catcggccag tagcgaactt tttgacctaa agtcgcgaag acgttacgga
     6421 aaaaacgggc gtcagcatca ttgctaagcg ccattatcgg gatcatcaac agcagcgcca
     6481 gggtattgac cttgcgcagg ttatcaatca cgccgccgat atctttgcgc aagccgtaaa
     6541 tcaaaccggc cagtgcgatg atggtggcta tggtcagaaa cagcttgaca cgcgactgtt
     6601 tgagcggatt actttcactc ataaactaac tactgaccat tatagaacgg cttcgcctta
     6661 taagacaccc cttcagggtt tcccatcgcg ccgggcgcgg aatgaatccg acgctcgggc
     6721 tgcccttccc gaagaacaag gtcagttttc gtattacaat agggactatg gcagggtttg
     6781 aggctaagag cctgtgcatt ttaggccgcc agccggcatt ggggctggcc gagctggagt
     6841 ctttatatgg gcctgagcac atccaaccgc tttccggcac agcgctgttg gatcttgagg
     6901 ccggagatat caattttaag cgcttgggcg gctgtatcaa ggttgccaaa gttctggcca
     6961 gcctgcccgg cgccggctgg aacgacatta agaaatacct gctggaaaac gtgcccaagc
     7021 acctggcgca cgcgccggcc ggaaaattca cactcggttt gagtttttat ggcttggctc
     7081 caccggtgac agaaatcaac aaactggcac tagatatcaa gcgggccagc ggccggccgc
     7141 tgcgcgtggt tcccaataaa tcggccaccc taaatagtgc ccaggtactg cacaataaac
     7201 tgaccagcaa aggcgcgtgg gaattgctgc tcgttaaaaa tggccagcgg accattctgg
     7261 cccagacttt ttttgtgcag gatattgagg cttacggcgc ccgcgaccag gcccggccta
     7321 agcgcgacgc ccgcgtaggt atgctgccgc cgaagctggc gcagataatt ataaatctgg
     7381 cggccggccc gctggaccat gaaattgaaa ataaaaccaa aatccgggtg ctggaccctt
     7441 tctgcggcac cggagtagtg cttcaggagg cgctgctgat gggctatagc gtgctgggca
     7501 gcgacattga gccgcggctg gtgggttaca gcgaagagaa tttaaagtgg ctggtagcta
     7561 gacagccgaa aattgaaggc aatgttgccc tggaagcagc agacgccacc agcgccacct
     7621 ggccgcgttt taccactgtc ggcagtgaag tttatctggg ccggccgctt gccaagctgc
     7681 ccgaaccggc caaacttaaa gagattatca gcgacgttga taccatcatc aaaaaattcc
     7741 tcaaaaacct ggcgccgcag ctaaaaagcg gccggcccgt ctgtctggca ctgccagctt
     7801 ggcgccaacc gaacggccag ctgatcaagc taccgatcat tgaccaatta acagatatgg
     7861 gatacaatta tatggattta aaacacgtgg gacgacaaga cttggtttat ttccgtgaag
     7921 accaggtcgt cgctcgccaa ttgataagga tagcaaggaa atagatgtca aaagtaaaag
     7981 ctggcggttc cagcagcaat gtccatgaca gccccggcca gcgcctgggc ctcaaggtgt
     8041 ttggcggcgc caaagtcaag gccggcgacg tgctggtgcg ccaggtcggc atgaccaagc
     8101 gctccggcgc cggtaccttt atgtcgcgtg attttaccat ccacgccgcc aaggacggca
     8161 ctgtcaaatt tagcaaacgg cgctttaagc gcttttccgg ccgcactatc ccccgcaccg
     8221 aagtctcggt agaataaatc aggcgttaag ctgctgtttg atgcgggtga tgatgtcggc
     8281 gataacaact tgtgatttca ccaaatagtc gtcgacgccg aggttttcgg cgcgctggcg
     8341 gtcagaatcc tggccgcggg cagtgaccat aatgattttc aaattggtag tttcaggcgt
     8401 gttgcgcaag atgtccagca catcaaaacc gccgactttg ggcatcatta tgtccagcac
     8461 cacgaggtcg ggtttgtatt caatggctgc ggatagagct tcttcgccgt cggccacccg
     8521 gcggacatca aaaccctcgg cttttagacg ggtcaaataa acatcggcca ggttgttatc
     8581 gtcctcaacc agcaggattt ttttgggctt ggcggccggt acgggctgtg attggccgct
     8641 gtcgctattt tctaaactca ttgaatttat gctagtttat ccgccgccga atgtctagcg
     8701 cttaagacag gctcgcacca gacccaaaaa cagcgggtgc ggccggttgg ggcgtgagcg
     8761 gaattccgga tgggcttggg tggccaggaa ataagggtgg tcacgagcct ctataacttc
     8821 taccagatgg ccgtccggac tcaagccgct ggccacaatt ccccagtttt ggtaacggtc
     8881 gcggtaggtg ttactgcact cgcagcggtg gcggtggcgc tcggttattt tctcctggcc
     8941 gtaggccttt cgggctttgc tgccggcggc tagaaccgtc gggtaatcgc ccagacgcat
     9001 agtgccgccg gtatttttct tatcgtgctg gtcggccata tagtcaatca ccttgcgcgg
     9061 gctcttggga ttcagctcca ccgttgtagc gtcatttagg ccggcgttgc gggcggcagc
     9121 gatgacagcc atctgcagcc ccaggcatag gccaagataa gggactttgt tatctaagct
     9181 gtagcgggcg gcgataattt taccttccag tccgcgttcg ccaaagccgc cgggaacgat
     9241 gatgccgtcc agagtcggaa gtatttcgcg ctcttttttg tccctttcca aagcctcggc
     9301 gttgacccag actatatcaa cgttaacctc gttagcccag gccgccgccc gcagagcctc
     9361 aaacacacag atgtaagtgt cctggttgtc tacgtatttg gcgatgatgc cgatacggac
     9421 agtcttgcgg tatttactga gagcgctttt aacgacccgc cgccaggccg ccaaatccgg
     9481 cttttgcagc cgccgcagac ccaggcggcc ggtgataacc tcggcaattt tagcttcttc
     9541 caagctcagc ggcacttggt aaatggtttg ggcgttgggc agcagggcga tggcttcttt
     9601 gtctacgccg gtgaacatac tcagcttgtt aagaatgcct ttgggaggct tttgctcgga
     9661 gcgggcgatc agcacatcgg gcacgacgcc caaaccgcgc agttcgcgca ccgagttttg
     9721 cgccggtttg gtcttgaact cttcactagt ccccaaatac gggatgtaaa ctacgtgcac
     9781 aaacagcgcg ttctcggcac ccaatctctg ccccatctcg cggatggctt ccaaaaagct
     9841 ctggctttct atgtccccta ccgtgccgcc gatttctacg atatgcacgt caaaccctcg
     9901 tccagtctcg gcgatcctgg ac
//
LOCUS       DASPTF010000024         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_463957, whole genome shotgun sequence.
ACCESSION   DASPTF010000024 DASPTF010000000
VERSION     DASPTF010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463957"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(504..788)
                     /locus_tag="VEH48_00765"
     CDS             complement(504..788)
                     /locus_tag="VEH48_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17938.1"
                     /translation="MNDTTAFIVVLAVFCTVITALLDWRLPASFRRYIAVSAAACWVA
                     TVWQFHDRISTGWQIAAYAGAAVLPFVIALIADVHYGSKEFDFILRGSRT"
     gene            complement(801..1058)
                     /locus_tag="VEH48_00770"
     CDS             complement(801..1058)
                     /locus_tag="VEH48_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17939.1"
                     /translation="MDSEPNTNRQREDTPRGTEKFLVGTGFLVFAALLAVAAVVAYFL
                     YFAASHARPLDIAAAIMLVAVILYGWQEPQLTAIWRRLRRR"
     gene            1183..1746
                     /locus_tag="VEH48_00775"
     CDS             1183..1746
                     /locus_tag="VEH48_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HXY17940.1"
                     /translation="MQPGQILDEAKTKLAAATEHFKQEIGKIRTGRAHPGMLDGLVIE
                     AYGQNMPLKSVAGISTPEPQLLQINPFDPNNLKAIADAIRNNESLGLTPTDDGRVVRV
                     AIPPLTEETRQQMVKILHQKLEDCLITARNARHEAIHQAEEAEKSKQIGKDQRFSLEK
                     QLDELLAKQKTELDGLAKAKEQEILTV"
     gene            1895..>2896
                     /locus_tag="VEH48_00780"
     CDS             1895..>2896
                     /locus_tag="VEH48_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXY17941.1"
                     /translation="MQVLLFALAIVLFIGLVIIHEWGHYIAAKRNGVQAEEFGLGIPP
                     RAWGKKLKNGVLFSVNWLPLGGFVKLKGEHDSDTKPGSFGAASLGAKTKIMLAGVFMN
                     LIAGLVILTILALTGLPKLLDTDYGAPANQFTVKSDTKVSKQEVLVGTVQAGSPASQL
                     GFASSDVIKSLESGGQTRQITSVDDLQNATSDFAGQNVVVTYQHNGKTLSKPVKLRSR
                     AEVLPSLKTANPKGYLGVAPVQLQIRRSTWSAPVVAVGFTGQLGWLTLQGLWHALSGL
                     GSAIAGTVTGNHAARENGQTQASSQVGGPVAIGAILWGYSYLGFSFVFTIIAVISLLL
                     "
BASE COUNT          676 a          785 c          828 g          607 t
ORIGIN      
        1 atttagggaa gccacacagc tacaagctga atagttatga ggggggcaag tacagactta
       61 acccaaactc ttcagcaacc accgcagcgg cttttgagac gggatttctc gatttcagta
      121 gtgcaattga tagctttcta gagatcgccg gcagcggcga cccaccgtcg tagcaaaata
      181 cttaacccct ctacctcgct cggggcaggt aagtcaagta tttttcaagg tatgaaaata
      241 gcggctgcgc ggccgcgagc aaataaatgc tgcaaacggc gcacatgccg ctatgggagc
      301 gagtactcta ttcggatttt aggtccgatt ttcgtcgctc ccgcgaacac gtcagatgcc
      361 ttgtgctagg catcttcatc tctggccgtg gatgaagccg gtgatgacca accacgcaag
      421 gaagagaagg acgagcaacg tcgcgaagtt gggcagcgtc agccgttttc gcttctgctc
      481 tcccgctggt gtctctggct cattcatgtg cggctccccc tgaggatgaa gtcgaactcc
      541 ttgctgccgt agtggacatc ggcgatgagt gcgatcacga atggcaggac ggccgctccg
      601 gcgtaggcag caatctgcca acccgtgctg atgcggtcgt ggaactgcca cacggtcgca
      661 acccagcagg ctgctgccga cactgcgatg tagcgccgga aactagccgg caatctccag
      721 tccaacagcg ccgtgatgac ggtgcagaaa accgccaaga cgacgatgaa tgccgttgta
      781 tcgttcatca caccctttcc ctagcgccgc cggaggcgcc gccagattgc agtgagctgg
      841 ggctcttgcc atccgtacag gatgacagca acaagcataa tcgccgcggc gatgtcgaga
      901 ggacgggcat ggctcgcggc aaagtatagg aaatacgcca cgactgccgc cacggctagt
      961 agtgccgcga agaccaggaa gccggtgccc acgaggaact tctccgtccc ccttggcgtg
     1021 tcttcacgct gccgattggt gttcggctca gaatccacag attcctccag gttttcaggt
     1081 gcgggccgac gggcccgcac gcttggccta tcaaccgatt gtcagattat aacacttatg
     1141 ttcggctttt tcaatacggg ccggccgttg tataatcagt gcatgcaacc tggccaaatt
     1201 ttggatgagg ctaagaccaa gctggcggcc gcgaccgagc attttaaaca ggagatcggc
     1261 aaaatccgca ccggccgggc acatccgggg atgttggacg gcttggtaat agaggcctac
     1321 ggccagaata tgccactgaa aagtgtcgcc ggcatcagca cgccagagcc gcagctgctg
     1381 cagatcaatc cttttgaccc gaataacctt aaagctatcg ccgacgccat ccgcaacaat
     1441 gaatcgttgg gtttgacgcc tacagatgac ggccgggtgg tgcgggtcgc tattccgcct
     1501 ttgaccgaag agacccgcca gcagatggtc aaaatcctgc accagaaact agaggactgt
     1561 ttgattaccg cccgcaacgc ccgtcacgaa gccatccacc aggccgagga ggctgagaaa
     1621 tccaagcaga tcggtaagga ccagcgtttc agcttggaaa aacagctgga cgagctattg
     1681 gctaaacaaa aaaccgagct ggacggcttg gccaaggcca aagagcagga gattttgaca
     1741 gtttaatcgt ttaccgtttg ccgttgaccg tttacagtgc gttcacagtt taccgtttac
     1801 aatggttaac ggaaaacgaa taacgaataa cggataacgg ataacgcaag gttaaaggtg
     1861 aagggtgaag ggataaaggt tatactataa cggtatgcaa gttttgttgt tcgctttggc
     1921 gatagttcta tttatcggct tggtgattat ccacgagtgg gggcactata tcgccgccaa
     1981 acgtaatggc gttcaggccg aggagttcgg cctgggcatc ccgccgcggg cttggggcaa
     2041 aaagctgaaa aacggcgtac ttttcagcgt taactggctg ccgctgggcg gcttcgtaaa
     2101 gcttaagggc gagcacgact cggacactaa gccgggcagt tttggcgcgg cttctttggg
     2161 cgccaaaacc aagattatgt tggccggcgt gttcatgaac ttgatagccg gtctggtgat
     2221 tttgactatc ctggctctga ccggcttgcc taaactcctg gatacggact acggcgcgcc
     2281 ggccaaccag ttcactgtca aaagcgatac taaagtctca aaacaagaag ttctggtcgg
     2341 cacggtgcaa gccggttcgc cggccagcca gctgggtttt gccagcagtg acgtcataaa
     2401 aagcctggaa tcgggtggtc aaactcggca aattacatcc gtggacgatc tgcaaaatgc
     2461 cactagcgat tttgccggcc agaacgtcgt agtgacctac cagcataacg gcaaaacttt
     2521 gagtaaacca gttaaactgc gcagtcgggc agaggtcctg cccagcctca agaccgccaa
     2581 ccctaaaggc tatctgggag tagcgccggt ccagctgcag atccgacgca gcacctggag
     2641 cgcgccggta gtggctgtgg gctttactgg ccagctgggg tggctgacct tgcagggtct
     2701 gtggcacgcc ctttcaggcc tgggctcggc catcgccggc accgtgaccg gtaatcacgc
     2761 ggcgcgcgaa aacggccaga cccaggctag cagccaggtc ggcgggccgg tggccatcgg
     2821 cgccatttta tggggctaca gttacttggg cttcagcttt gttttcacga ttatcgccgt
     2881 catttcgtta ttgcta
//
LOCUS       DASPTF010000025         8917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1902305, whole genome shotgun sequence.
ACCESSION   DASPTF010000025 DASPTF010000000
VERSION     DASPTF010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902305"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VEH48_00785"
     CDS             complement(<1..464)
                     /locus_tag="VEH48_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HXY17942.1"
                     /translation="MTERYSQPRTVAVTGLGTINSAGNNVKDYWDAVTSGRREGIITN
                     ERAWLLAQTPDTYHKKLRSSLIGRVKSDDLAGDLLALEELAYQHFDRKYIDRYLSESA
                     ILGAIAAAEAMRDAKAHRAVDPKRLAFVLGTGIGGGIEISTYQSVMEAGGSLP"
     gene            complement(473..1444)
                     /locus_tag="VEH48_00790"
     CDS             complement(473..1444)
                     /locus_tag="VEH48_00790"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HXY17943.1"
                     /translation="MANQTPVLGVEIAGWGGALPAESLTNDELIARYSLDSSDAWIRE
                     RTGIERRSICSEDENLVALATDAGRRALESTGLTPPRTIDKLFLGSTTPYRDVPGSHP
                     AITRALGELGYSFTSSKESNTACTGFVTSLLDGYRSFAVDGIDSALVIGADTLSSITD
                     YTDRSTAILFADGAGAVAMQRTDRDSGLLGWHEETDGTVEEILFCERGGSIQMDGQEV
                     FKRAVKVMIKAGEIALEKSETDVEALALTVPHQANLRIIELAGRKLGIPLHRMAVTIN
                     KHGNTSSASIPLALAEAADAGRIARGAKIMMVGFGAGMTAAAAVIEW"
     gene            complement(1503..2447)
                     /locus_tag="VEH48_00795"
     CDS             complement(1503..2447)
                     /locus_tag="VEH48_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXY17944.1"
                     /translation="MSENLPHPVIELLETRRQQIEDDHVLGLVVEGGGLRGVLTAGML
                     ISMEQNGYGPDIFDFVVGSSAGAFNAAYFLSGNVSEGATIYSEHSAQHGFIDLKAWLK
                     LRERRPIMDVSIHTNHLLEGVVPLDWENIIASEKLHIAVSDPHEIGTRVLPPPSDKDE
                     LKRNLRATAHIPILAGRPTLIDGLPYYDGSLTAPLPIEEAISLGATHVLALSSRSLKK
                     WRNKQTRIERLASRLYNLRYPGIDAAVSQGVETAIQRVQYLIEARDNPEDIPFIYTVD
                     APENIQIRQFEADAAKLADGVRLGKIALASCLPPASQR"
     gene            complement(2456..2532)
                     /locus_tag="VEH48_00800"
     tRNA            complement(2456..2532)
                     /locus_tag="VEH48_00800"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2496..2498),aa:Val,seq:gac)
     gene            2926..4161
                     /gene="thrS"
                     /locus_tag="VEH48_00805"
     CDS             2926..4161
                     /gene="thrS"
                     /locus_tag="VEH48_00805"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXY17945.1"
                     /translation="MDEAKTGDGPDHRRLGKELDLFVFSDLVGAGLPMFTPRGTVLRD
                     ELLGFSEELQRKAGYEKVAIPHITKDELYKKSGHWDKFGGELFLVKSQETKDQMVLKP
                     MNCPHHIQIYASRPRSYRELPIRYMENTIQYRDEKKGELNGLSRVRSISIDDAHIFCQ
                     LDHIEQEFTTIMEMIKEMYRVFGMEFRARLSFRDDSEGYLGDKEHWDQAQKIIEDVAK
                     KLGLKYDIAEGDAAFYGPKIDIMVTDSLGREWQCATEQLDFFMPERFGLTYIDKDGIE
                     KPVVMIHKALLGSFERFLSVYIEHTNGHFPLWLAPEQLRVATLNNEEPVEKLAKEIVD
                     RGKGLGLRVGLDDSNESVGKKVHDAEAMKVPYTVVVGANEVESSRLTPRHRSDLPELP
                     ESSVEELLEKLAQDAQARK"
     gene            4142..4756
                     /locus_tag="VEH48_00810"
     CDS             4142..4756
                     /locus_tag="VEH48_00810"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17946.1"
                     /translation="MPKPANKPVIAVDIDDVIAAHAPAFIAYTNEKWGTHLTINDYQD
                     HWGEVWKVDFEEREKRAEEYHRTDYIGTYGVIGGASATLGKLRDKYKLVILTTRRRSI
                     EQLTRDWIEKYYPGIFDDVVFAGFFDNPTRSSIHMTKGELAEQIGAGYLIDDQLKHCL
                     AATEAGIKALLFGDYPWNQTDNLPEDITRVRNWQEVLEYFNEKG"
     gene            4743..5087
                     /locus_tag="VEH48_00815"
     CDS             4743..5087
                     /locus_tag="VEH48_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HXY17947.1"
                     /translation="MKRVDADFRQRVYQIVGSIPRGRVMTYGQIAALAGAAWAAWEVG
                     QIAHTGPSELPWQRVVNKKGGLARGYTPGGYEAHKQDLEAEGVAVDSDYRVDIDRLLW
                     FPPHKEQNSLPL"
     gene            5045..5923
                     /gene="miaA"
                     /locus_tag="VEH48_00820"
     CDS             5045..5923
                     /gene="miaA"
                     /locus_tag="VEH48_00820"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXY17948.1"
                     /translation="MVPAAQRAKLIAIVGPTASGKSNLAMRLAKDFNGEIVSADSRMV
                     YTGMDIGTGKPTDREQKLVPHHLINIVNINQFFSAAEYKALAQKAIKSIQAREKTAIL
                     VGGSGLYVDAVLFDFDFRPPPSRSERRRLEAMTVEQLNAEIAKRKLTPPLNYRNKRHL
                     IRCIETGGAQNIRKAMRPDAIVIGILPGSEELKRKISSRNSKMINNGLEQEVRGLTQR
                     YGKEARSLDAIGYREWQDYFDGKISRQDVKNLITNNSIAYARRQKTWFKRNEHIKWFT
                     SPQAAYKYAKNSLLNT"
     gene            6073..6711
                     /locus_tag="VEH48_00825"
     CDS             6073..6711
                     /locus_tag="VEH48_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17949.1"
                     /translation="MIEQLFGSKTRVKLLQLFYANPNRSFYVREITRKVDEQINSVRR
                     ELSNLLSIGIITSDANNNRLYYEVNQKFKYYLPLAQIFGGLKAAPAPASKKSGAKSTA
                     EEPRIAAVGPEHPMAKGLRGTGRVDLALLTGQFTRDELSGVDLLVVGDINQSKLDRFM
                     EELEKAENKELRYTSMPLDTYQYRVQINDRFITNVLGAKKQVIINNQDLLVE"
     gene            6730..7359
                     /locus_tag="VEH48_00830"
     CDS             6730..7359
                     /locus_tag="VEH48_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXY17950.1"
                     /translation="MELSFYGANCLRLATRKATIAVDDNLAAVGLKAITKPDDISLRT
                     SVKLIPKHDSARFSADMPGEYEMSGVVIHGVAAKAHMDKEGEHTAVIYTVEADDLKVA
                     VIGHIYPELSDDQLEQIGLVDIAVIPVGDSGYTLDGAGALKIIKALEPKVVIPTHYAD
                     KAVKYEVPQVELAEALKNLGMEPAETLDKYRPKPAELTDTTRLIVLNRQ"
     gene            complement(7361..7549)
                     /gene="rpmB"
                     /locus_tag="VEH48_00835"
     CDS             complement(7361..7549)
                     /gene="rpmB"
                     /locus_tag="VEH48_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXY17951.1"
                     /translation="MKRLDFDTKRKQHGSNVSFSQRHTKRVWKPNLQVKTLTVDGKKV
                     KVRISTTAIRSLKKKGLL"
     gene            7617..8252
                     /locus_tag="VEH48_00840"
     CDS             7617..8252
                     /locus_tag="VEH48_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXY17952.1"
                     /translation="MTVDLQTIAILLVVLLFSMSIHEMMHAFTADWLGDDTARIMGRV
                     TLNPLAHIDPFFTVALPLLLLLSHSPYLFGAAKPVQVNFHRLKYDEFGGAIVGMVGPL
                     TNLVIAVVAALIFNHLSLVNGTAVYTIFEITILLNVGLFIFNSIPWPPLDGSRLLYAF
                     APRPLQELMESIEHLGMTGLVVFILIFYSFGGGIGNLMLHVVHYLAPTLIT"
     gene            complement(8556..8631)
                     /locus_tag="VEH48_00845"
     tRNA            complement(8556..8631)
                     /locus_tag="VEH48_00845"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8596..8598),aa:Pro,seq:ggg)
     gene            8677..>8917
                     /locus_tag="VEH48_00850"
     CDS             8677..>8917
                     /locus_tag="VEH48_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HXY17953.1"
                     /translation="MKTLKFDHEQARAIATGQKNSTWRLYDDKDLSVNDDIKIIDKID
                     PKSSGSWKIIGQARVTEIVEKLLGAVNAADIRLNDL"
BASE COUNT         2269 a         2223 c         2281 g         2144 t
ORIGIN      
        1 ggtaaactgc cacctgcttc catcactgac tggtaggtgc ttatctctat cccgcctccg
       61 atgccggtcc caagaacgaa agccaatctt ttcgggtcaa cggctctgtg ggcttttgcg
      121 tcgcgcatag cttctgcggc cgctatcgcc cccaatatgg cgctctcact caagtatcga
      181 tcaatatact tcctatcaaa gtgctggtat gccaattctt caagcgccag caaatcgcct
      241 gctaggtcat cacttttaac acgtcctatt aagctggatc gcaatttctt gtgataggta
      301 tccggggtct gagcaagcag ccacgcccgc tcattagtaa ttatgccttc tctgcggcct
      361 gatgttaccg catcccaata gtctttgacg ttgttgcctg cggaattaat tgtgccgaga
      421 ccggttacag ctactgttcg cggctgagag tatctttctg tcatatgtcc cctcaccact
      481 ctattactgc agcagcagct gtcatgcccg ccccaaaccc caccatcatt attttagctc
      541 cgcgtgcaat acgaccagcg tcggcggcct cggctagggc caaggggata gaagctgagg
      601 atgtattacc atgtttattg atcgtcaccg ccatccggtg caatgggatg cctaattttc
      661 tgcccgcgag ttctataatc cgtaaattag cttgatgggg aacggttagc gccaaggcct
      721 ctacgtcagt ttctgacttt tccagcgcta tctcgccagc cttaatcatt actttgaccg
      781 ccctcttaaa cacctcttgg ccgtccattt gtatactgcc gccgcgttcg caaaaaagga
      841 tttcctcaac cgtgccatca gtttcttcgt gccagccgag taatccgctg tcgcggtctg
      901 ttctttgcat agcgaccgca cctgcgccat cagcaaataa tatcgcggta ctgcggtcag
      961 tataatcagt tatagaggat agggtatcgg ctccgattac taaagcgctg tcaatgccat
     1021 cgaccgcaaa gctacggtac ccatccaata aactggttac gaatccggta caagcggtgt
     1081 tcgactcctt gctggaagta aacgaataac ccaattcacc aagcgcgcgg gtaatggcgg
     1141 ggtgtgatcc gggaacatca cggtaggggg ttgttgaccc aagaaacaac ttgtcaattg
     1201 tacggggcgg agtcaagccc gtggattcca gtgctcgccg tccggcgtcg gtagccaaag
     1261 ctactagatt ttcatcttca ctacagatgc tgcggcgttc tatgccggtg cgctcccgaa
     1321 tccaagcatc ggaagaatca agactataac gcgcaataag ttcatcattc gtcagcgatt
     1381 ccgctggcag tgctccaccc cagcccgcaa tctctactcc caaaaccggt gtttggttgg
     1441 ccatatgttc tcctaagtac tgatactata agattattaa gacagtttgg caaactaaaa
     1501 tcctatcttt gtgaagctgg cggcaaacag cttgctaagg caatcttgcc aagtcgcact
     1561 ccgtcagcca actttgcggc gtcagcttcg aactgcctaa tctgaatatt ctccggcgcg
     1621 tcaaccgtat atatgaatgg gatatcttcg ggattgtctc ttgcctcaat caagtattgg
     1681 actcgttgta tggctgtttc taccccttgg ctgacagctg cgtctatgcc ggggtatctt
     1741 agattgtaaa gacgagacgc cagtctctct atacgggttt gtttgttccg ccattttttt
     1801 agactccggc tagacaaagc taatacgtgt gtcgcgccta aacttatagc ttcttcgatg
     1861 ggcaacggag ccgttaaact accgtcataa taaggcagcc catcaattaa tgtcggccgg
     1921 ccagccagaa tcggaatatg cgccgtcgct ctcaaattac gcttcaactc gtctttatct
     1981 gatggcgggg gcaggactct tgtgccaatc tcgtgaggat cagaaactgc tatatgtaat
     2041 ttctcagagg caattatgtt ctcccaatcc agcgggacaa ctccttcaag tagatgattg
     2101 gtatggatgg aaacgtccat gatcggccgg cgctccctta actttagcca cgctttcaga
     2161 tctataaatc catgttgggc agagtgctcc gaatatatag tcgctccctc tgatacgttg
     2221 ccgcttaaaa aataggctgc attgaaagcg ccggcagagg agccgactac aaaatcaaag
     2281 atatccggtc cgtaaccatt ttgctccatg cttattagca tgcccgctgt tagcacccct
     2341 ctcaagccac ctccttcaac gactaaacct aaaacatggt cgtcttctat ctgctgtctt
     2401 ctggtttcca gcagttctat aacaggatgt ggtaagtttt cgctcataaa taaaatggtg
     2461 ggcggtacag gattcgaacc tgtcacctct tccacgtcaa ggaagcgctc tagccaaatg
     2521 agctaaccgc cctcgtcgga gccggggaaa cggctttcgt aacttcttag cagaagtcac
     2581 ttacagcctc gacccgtctc ctcctctcaa aaacgcaact cgctacagat cttacgtttt
     2641 tgtagaagac caagtcaatt atactatctt ggccaggtat aaattaaaag ttatcacaca
     2701 tggtataatt gaatggttga gggacctgtg gataaatggg taaaaaagta ctagccaaat
     2761 cctgctagta catatatact cctaaacagg ttgagttctg ccgatgtgtt aaccctcaca
     2821 agttggcttg aatgacgttg cgagatcatt caagcctttt tgtatggcaa gtcgcaaaac
     2881 gtaaagactt tttgtatcaa atctaaggtg ttatgataga cactaatgga cgaagcaaaa
     2941 acaggggacg gacccgatca ccgccggctc ggcaaggagt tagacttatt tgtattttcg
     3001 gatctagtcg gagccggttt accaatgttt actccccggg gaacggttct tagagacgaa
     3061 cttttaggtt tttctgaaga gttacagcgc aaagccggtt atgagaaggt cgcaataccg
     3121 cacattacca aagacgagct ttataagaaa tcgggccact gggacaagtt cggcggagag
     3181 ttgtttttgg tcaaaagcca ggagaccaag gaccagatgg tacttaagcc gatgaactgc
     3241 ccgcatcaca tacagatcta cgccagccgg cccaggagtt accgcgagct gcctatccgt
     3301 tatatggaaa acaccatcca gtacagggat gaaaagaaag gcgaactgaa cggcttgtcg
     3361 cgcgtgcgct ccatctctat agacgacgcc cacatcttct gccagctgga ccacattgaa
     3421 caggaattta ccaccatcat ggagatgata aaggagatgt atcgggtgtt tggcatggag
     3481 ttccgtgccc ggctgtcttt ccgcgatgat tccgaaggct atctgggcga taaggaacat
     3541 tgggaccagg cccagaagat tattgaagat gtagccaaga aactggggct gaaatacgat
     3601 atcgctgagg gcgacgcagc gttttatggg ccgaaaatag acattatggt taccgattct
     3661 ctgggacggg agtggcaatg cgccaccgag cagctggatt tcttcatgcc ggagcgtttt
     3721 ggactgactt acattgataa agacggtata gaaaagccgg tggtgatgat ccacaaggcc
     3781 ctgctgggtt cgtttgagag gttcttatct gtttatatag agcacaccaa tggtcatttc
     3841 ccgctgtggc tggcgccgga gcagctgcgg gtggctacgt tgaacaacga ggagccggta
     3901 gagaagttgg ctaaggagat agtagatagg ggaaagggtc tagggttgag ggtaggattg
     3961 gatgattcca atgagtcggt cggcaagaag gtccacgatg ccgaggccat gaaagtgccg
     4021 tatacggtgg tggttggcgc taatgaggtg gaatctagcc gcctgacccc gcggcaccgc
     4081 tcagacctgc cggaactgcc cgagtcttcc gttgaggagc tgctggaaaa gcttgcccaa
     4141 gatgcccaag cccgcaaata agcccgttat cgccgttgac attgatgacg tgatagccgc
     4201 gcatgccccg gcattcatcg cctataccaa tgaaaaatgg ggtacacacc tgacgattaa
     4261 cgactatcaa gaccactggg gcgaggtctg gaaagtagat tttgaagaga gagagaagcg
     4321 agctgaagag taccatagaa ctgattatat tgggacttac ggtgtcatcg gtggcgcttc
     4381 tgcgacgctg ggaaagcttc gggataagta caagctggtc attttgacca cacgcagaag
     4441 gtccattgaa cagctcacgc gggattggat agagaagtat taccctggta tcttcgatga
     4501 tgttgtcttt gccggatttt ttgataatcc gacacgatcc agcatccata tgactaaagg
     4561 tgagcttgcg gaacagattg gtgctggcta cctgatagat gatcaactaa aacactgcct
     4621 ggctgctaca gaggcaggaa ttaaggcctt gctgtttggt gattatcctt ggaatcagac
     4681 cgataatctg ccggaagaca tcacacgcgt tagaaactgg caggaggtgc tggaatactt
     4741 caatgaaaag ggttgacgcg gattttaggc agagggttta tcaaatagtc ggttcaatac
     4801 caagagggcg ggtgatgacg tatgggcaga ttgccgcact agctggtgcc gcttgggctg
     4861 cctgggaagt cggccagatc gcccacactg gcccatctga gctgccctgg cagcgggtgg
     4921 tgaataagaa aggtgggcta gcccggggct acacccctgg cggctacgaa gcccataaac
     4981 aggacttaga agctgagggc gtggctgttg atagcgatta ccgggtggat atagataggt
     5041 tattatggtt cccgccgcac aaagagcaaa actcattgcc attgtaggcc ctacagcctc
     5101 gggcaaatct aacctggcta tgcgcctggc taaagatttt aatggcgaga tagtctccgc
     5161 tgactcccgc atggtttata caggcatgga tattggtact ggtaagccta ccgacagaga
     5221 acaaaaacta gtgccgcatc atctgataaa tatagttaat atcaatcaat ttttttcagc
     5281 ggctgaatac aaagcattag cacaaaaagc cataaagagc attcaggcta gggaaaaaac
     5341 agccattttg gttggcggta gcggactata tgtagatgca gtgttatttg actttgattt
     5401 caggccaccc cccagtcggt ctgagcgacg ccggctagaa gctatgactg tcgaacagct
     5461 taatgcagaa atagcaaaga gaaaattaac accaccgctt aattacagaa ataaaaggca
     5521 ccttatccga tgtatagaaa ccggaggagc tcaaaacatc aggaaagcca tgcgtccaga
     5581 tgcaatagtg attggtatat tgccgggatc cgaagaactg aaacgaaaaa tatccagccg
     5641 taattccaag atgattaata acggtctaga acaagaagtt cgcggtttga cgcaaagata
     5701 tggaaaggaa gcccgatctc ttgacgccat aggctaccgt gaatggcagg attattttga
     5761 tggcaaaatt agtcgtcaag atgtcaagaa tctgatcact aacaacagta ttgcctacgc
     5821 ccgccggcaa aaaacctggt tcaaacgcaa cgagcacata aaatggttca ccagcccgca
     5881 agcagcctat aagtacgcca aaaacagttt attgaacacc taatttacaa tattttgcta
     5941 taattaggcg tgagatggtg agtaatattt ttcatcggga agggcagccc gagtgtcgga
     6001 ttcattccgc gcccggcgcg atgggaaacc ccgaaggggt gtcttataag gcggagcgaa
     6061 agcgtagccg ccgtgattga gcaactgttt gggtccaaga cgcgggtaaa gctgctgcag
     6121 ctgttttatg ccaatccgaa ccgttcattt tatgtgcgcg aaatcacccg caaagtggac
     6181 gagcagataa attcggtacg gcgtgagctg tccaacctgc tttctatcgg cattatcact
     6241 tccgacgcca acaacaaccg gctttattac gaagttaacc aaaagtttaa gtattatctg
     6301 ccgctagcgc agattttcgg cgggctgaag gccgcgccgg cgccggcaag caagaaaagc
     6361 ggggccaaat cgaccgccga ggaaccgcgg attgccgccg tggggcccga gcacccgatg
     6421 gccaaaggcc tgcgcggcac cggccgggtg gacctggcgc tgctgaccgg ccagttcacc
     6481 cgcgacgaac tgtcgggcgt ggacttgctg gtggtaggcg acatcaacca atccaaactg
     6541 gaccgcttca tggaagagct ggaaaaagcc gagaataaag agctgcgcta cacctccatg
     6601 ccgctggata cctatcagta ccgtgtgcaa attaacgacc gcttcataac caatgtgctg
     6661 ggtgccaaaa aacaggttat aattaataac caggacctgt tggttgaata aaaaggagcg
     6721 aagctaaaag tggaactgtc gttttacggt gctaactgcc tgcgtttggc taccagaaaa
     6781 gccaccatcg cggtggatga taatttggcg gccgtcggcc ttaaagccat caccaagccg
     6841 gatgacataa gtctgcgtac ctctgttaaa ttgattccta aacatgacag tgctcgcttt
     6901 agcgccgata tgcccggcga gtacgaaatg tccggcgtgg ttatccacgg cgtggctgcc
     6961 aaggcgcaca tggataaaga aggcgagcat acggccgtga tttacaccgt tgaagccgac
     7021 gacctcaagg tcgccgtaat cggccacatt taccccgaac tatcggatga tcagctggaa
     7081 cagatcgggc tggtggatat cgcggtgatt ccggtgggcg atagcggcta caccctggac
     7141 ggggcagggg cgctcaaaat catcaaagcc ctggagccca aagtggtgat accgacacat
     7201 tatgccgaca aagccgtgaa atacgaagtg ccgcaggttg agctggccga agccctcaag
     7261 aatctgggca tggagccggc cgaaaccctg gacaagtacc ggcccaaacc ggccgagcta
     7321 acagatacga cccggctgat tgtcctcaac cgccaataga ttaaagaagg ccttttttct
     7381 tgaggctgcg gatggcggtg gtgctgattc tgactttgac ctttttacca tccacggtca
     7441 gggttttgac ctgcaaatta ggcttccaca cccgcttggt gtgtcgctga gaaaaactga
     7501 cattactgcc gtgctgcttg cgcttggtgt caaaatctaa ccgtttcatg attcaaggtg
     7561 ttattttaca gatgtaaacg gctaaagtca atcaccttgg gttatactaa agcgatatga
     7621 ccgttgattt gcagaccatt gccattttgc tggtggtatt gctgttttcc atgtccatcc
     7681 acgagatgat gcacgccttt accgccgatt ggctgggcga tgacaccgcc cggattatgg
     7741 ggcgggtgac gcttaacccg ctggcgcaca tcgacccctt ttttacggtc gcgctgccgc
     7801 tgttgctgct gctttcccac agtccgtatc tgttcggcgc cgccaagccg gtccaggtga
     7861 acttccaccg gctaaaatac gatgagttcg gcggagccat tgtgggtatg gtcgggccgc
     7921 tgaccaatct ggtaatcgcc gtggtcgcgg cgctgatttt caatcacctg agcctcgtaa
     7981 acggcacggc agtctacacc attttcgaga taacaattct gctcaacgtc ggcctgttta
     8041 tctttaattc catcccgtgg ccgccgctgg acggctcgcg actgctgtac gctttcgcgc
     8101 cgcggccgct gcaggagctg atggaatcca tagaacatct gggaatgaca ggcttggtag
     8161 tttttatctt gattttttac tcctttggcg gcggtatcgg caacctgatg ctgcacgtcg
     8221 tccactacct cgcgcctacg cttataacct agccgtttac cgttcaccct tcaccgttta
     8281 ccatactttt tccgtttacc gttcaccatg gctcatggaa aacggatcac ggacaacgca
     8341 gggaaaacgg ataacggcta atggtataat ggaattggtc ggtgagagac tagcacaata
     8401 aattttctga acagttagtt ccttgaggga gttcaacatt tgcaccgggc cgtggcccgg
     8461 gcgtgaggac acccactttg caaacgcaga gtaaattgag tgctttctga ccgaccttca
     8521 tacaaaaaac tcttcaatct gaagagtttt ttgtatggtc ggggatgcgg gatttgaacc
     8581 cacgacctca cggcccccag ccgtgcgcgc taccagctgc gccaatcccc gtcgtcggaa
     8641 ccgaaacagt accattatat ctgttattat tggtgggtga aaaccttaaa atttgaccac
     8701 gagcaggccc gggctatcgc caccggtcag aaaaactcca cctggcggct gtatgacgac
     8761 aaagacctat ctgttaatga tgacatcaaa attatcgaca agattgatcc aaaatcgtcc
     8821 ggcagctgga aaatcatcgg ccaggccagg gttaccgaaa tcgtggagaa gttgctgggg
     8881 gcggtgaacg ccgcggacat acgactcaac gatctgg
//
LOCUS       DASPTF010000026         4687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1904012, whole genome shotgun sequence.
ACCESSION   DASPTF010000026 DASPTF010000000
VERSION     DASPTF010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904012"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VEH48_00855"
     CDS             complement(<1..271)
                     /locus_tag="VEH48_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17954.1"
                     /translation="MQDIIPPPGWQKPKRPKPQPMRSLRLRGQSVDDARNRLRARRTL
                     PEAKIDETIALDVPEKPAGAKKRRFGWWRKLNRNLRFAIIALVLLL"
     gene            complement(295..1719)
                     /gene="gltX"
                     /locus_tag="VEH48_00860"
     CDS             complement(295..1719)
                     /gene="gltX"
                     /locus_tag="VEH48_00860"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXY17955.1"
                     /translation="MVRTRFAPSPTGALHAGTVRTALFAWLTARHAGGQFLLRIEDTD
                     QTRGVEGAVSNIIDSLHYLGLDWDEGPDEDGEYGPYIQSQRLNIYKEWAQKLIDKGRA
                     YSDPYTKDELDEFRAQAVAQKKPFVYRDHRPADPPAWNGSTPLRFKSEPKAYKWHDEV
                     MGDLSTGPEVVDDFILIKSDSYPTYNFAHIIDDHLMQISHVVRSQEFISSMPIFLNLY
                     DALGFDYPVFATVPLVLATEGNKKLSKREGAKQLLDYQKMGILPEALMNCLATTGWND
                     GSEQEIFSRDELIAKFDLGRVQKSGARFDEQRLLWMDGHYIRQTPLEALYAASEGFWP
                     ASAAKYDNAYKKQVLGLIQERLKYLAEIPALSQFFFEDLPIDRKLIDENKQLQGLDRQ
                     QLRELLGKSQDKLAQTDFSVEKLTQTLNGLLQETGQKPGVLFSLIRVATTWAPASPGL
                     AETLSLLGKDKTLARLHQTIESLS"
     gene            complement(1775..2845)
                     /locus_tag="VEH48_00865"
     CDS             complement(1775..2845)
                     /locus_tag="VEH48_00865"
                     /inference="COORDINATES: protein motif:HMM:NF025643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HXY17956.1"
                     /translation="MAKNTSIIELNGNKYDAVTGQLLGAVKRVAGQVKPVAGVIDGFV
                     RKPVAAIAAKQPTPGRKVQKAGRRPQKSRTLMRGAVAKYAEARKTVPGLRLKARSDRP
                     KAGQLLRAKVVGRTDRISRFGAAGNVMKSEALVGQVLPPAVARSSRGSSSASSAQAMP
                     SLVASISPHRLERLLDEALLRADSHREMLKRQLAGNSPWGRIKLMPRWLSVGASLLVV
                     LLLGGFFAWQNIPQVSMRVAASKAHISAAVPAYTPSGFRFAAPASNIAKAVTISYQAA
                     GDSGQKYSISQQASSWDSSTLASSVNSQSDQVQTSQVNGTTVYIYGSDNNAAWVDNGI
                     LHTLKDQANLSVDQIKDIVGSM"
     gene            2970..4478
                     /locus_tag="VEH48_00870"
     CDS             2970..4478
                     /locus_tag="VEH48_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HXY17957.1"
                     /translation="MDFLDPKKKRNYKIRLIIGYVLVAIALVLATVVLVYGAYGYSIN
                     TKTGSVIQNGLLFVDSKPGGATIYLNSQRHGTTSARLVLPSGNYTLKLTKTGYRNWQR
                     SISLEAQTIDRFVYPFLFPTKPRVTNLKTYVSVPPLVTETPDRHWLLVQLPNSDPSVV
                     SFDQFDTSKLDQTQSPTVVNLPAGILTNTDLPDNSLTEVAWSTDNSHLLLKHGYQGGS
                     EFIVFNRSVPSDSFNVNKALKINPTDVELRNDSVSQLYIYDAAAESLSVADTTKDVVD
                     KPFLTHVLAFKGYGTSLITYVTDNKMPAGQVQARIWDNGPTYALATLAAGDHYLIDAA
                     QYQSDWYYVAGSSGNDHIFLYKNPLSSLTAGDKALSFISLRVAGADQVGFSANTRFVE
                     AESGQQFGVYDFETMTRYQYTVQAALAGPMHWMDGHRLIGASNGQVFVMDYDGTNQQS
                     LTITDFALGGYFSNDYNHLLTTAPASDGTSVILQNVDMRAGADLPPAPSSGS"
     gene            complement(4475..>4687)
                     /locus_tag="VEH48_00875"
     CDS             complement(4475..>4687)
                     /locus_tag="VEH48_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17958.1"
                     /translation="LGPTDKAATFSLITCDPPGTSNNRLVVIGEQISPDPGGNLVASG
                     QNKLATESAVIPGNSPSLWSRIVNLF"
BASE COUNT          985 a         1328 c         1280 g         1094 t
ORIGIN      
        1 aaagcagcag caccaacgca atgatagcga aacgcagatt gcggttgagt tttctccacc
       61 agccgaagcg ccgcttcttg gcaccggccg gcttctccgg gacgtccagt gcgatagtct
      121 catctatctt ggcctcgggc agcgttctgc gggctcttag ccggttccta gcgtcgtcaa
      181 cagattggcc gcgcagccgc aaagagcgca tcggctgcgg tttgggccgt tttggctttt
      241 gccacccggg cggcgggata atatcctgca tcgcacttat tgtaacagct taagctaact
      301 caaactctct attgtttggt gcagacgggc gagggttttg tctttgccca gcaggctgag
      361 agtttcggcc aagccgggac tggccggcgc ccaggtagtg gcgacgcgga tcagactgaa
      421 gagcacgccg ggtttctggc cggtttcctg cagcagaccg ttaagcgtct gggttaattt
      481 ctctaccgag aagtcggttt gcgccagctt gtcctgtgat ttacccaaaa gctctctcag
      541 ctgttgcctg tcaagacctt gcagctgctt gttctcatct attaatttcc tgtcaatggg
      601 caaatcttca aagaaaaatt ggcttaaagc cgggatttcc gccagatact ttagccgctc
      661 ctggataagg cccagaacct gttttttata agcattatca tatttggccg ccgaggccgg
      721 ccaaaagccc tcagaagccg cgtacagcgc ttccagcggt gtttggcgga tgtagtggcc
      781 gtccatccac agcaggcgct gttcgtcaaa acgggcgccg cttttttgca ctctgcccag
      841 gtcaaatttg gcaataagtt catcgcgtga aaaaatctcc tgctctgagc cgtcgttcca
      901 gccggtcgtc gccaaacaat tcatcaatgc ctcgggcaga atccccattt tctggtagtc
      961 aagcagctgt ttggcgcctt cacgcttact gagtttttta ttgccttcag tggctaaaac
     1021 gagcggaacc gtggcaaaga ccgggtaatc gaatccaagt gcgtcgtata ggtttaaaaa
     1081 aatcggcatt gaggagataa actcttgcga gcggaccaca tggctgattt gcatcagatg
     1141 atcatcgata atatgggcaa aattgtaggt gggatagcta tcggacttaa tcagtatgaa
     1201 atcgtccact acctctgggc cggtagataa atcgcccata acttcgtcgt gccatttgta
     1261 agctttgggc tcggatttaa agcggagcgg cgttgaaccg ttccaggcgg gcggatcagc
     1321 cgggcgatgg tcgcggtata caaacggttt tttctgggct accgcttggg cacggaattc
     1381 atccaactca tccttggtat aggggtcgct gtaagctcgt cctttatcaa ttagcttttg
     1441 cgcccactct ttatagatat taaggcgctg gctttggatg taagggccat attccccgtc
     1501 ttcgtccggt ccttcatccc agtccaggcc gagatagtgt aaggagtcaa taatattgct
     1561 gacagctccc tctacccccc tggtctggtc agtatcttcg atcctgagca aaaactgacc
     1621 gccggcgtgc ctagccgtta gccaggcaaa tagtgccgta cgaaccgtgc cagcgtgcaa
     1681 tgcgccggtg gggctgggag caaaacgggt cctgaccata tcatcagtat acttcggcta
     1741 ggggctagcg actaggggct ggggtctagc ccagctacat actgccgaca atatccttga
     1801 tctggtctac ggataaatta gcttggtcct ttaaggtgtg taagatgccg ttgtcaaccc
     1861 aggcggcgtt gttgtctgaa ccgtaaatat aaaccgtggt gccgttaact tgcgaggttt
     1921 gtacttggtc gctctgggag tttacgctgg aggctaaggt ggagctgtcc cagctggagg
     1981 cttgctggct gatggaatat ttttgaccgc tgtcgccggc ggcctgatag ctgatggtca
     2041 cggccttggc gatgttgctg gcgggagccg cgaaccggaa accggatggc gtataagccg
     2101 gcacggcggc gctgatgtgg gctttactgg ctgccacgcg catggatacc tgaggaatat
     2161 tctgccacgc aaaaaatccg cccagcagca gaacgactaa caaactggcg ccgacgctca
     2221 gccatctggg cataagtttg atacggcccc aagggctgtt gccggccagc tggcgcttca
     2281 gcatttcgcg gtgcgaatcg gcccgcagca aagcttcgtc cagcagccgc tccaaacggt
     2341 ggggcgagat actggctacc aagctgggca tggcctgggc gctgctggcg gatgaactgc
     2401 cgcgcgatga gcgggcgacc gcgggcggca gaacctggcc gaccaaggcc tcacttttca
     2461 taacgtttcc ggcggcgcca aaacggctga ttctgtcagt ccggcccacg actttggcgc
     2521 gcagcagctg gccggctttg ggacggtcgc tgcgggcttt gagcctcaaa ccggggacag
     2581 tcttacgagc ctcggcgtac tttgccacgg cgccgcgcat cagggtgcgt gatttctgcg
     2641 gccggcggcc ggctttttgc actttacgcc ctggcgtcgg ctgtttggcg gctatagccg
     2701 cgaccggctt tctgacaaag ccgtctatca caccggcaac cggtttaacc tggccggcta
     2761 ccctctttac ggctccgagc aactgcccgg tgaccgcgtc gtacttatta ccgttcaatt
     2821 ctataatgct ggtgttcttc gccacttggc tactgatctg cctctttttg gtctaacggc
     2881 catcatacac ccacaaagca gcggctgcaa gtattgggcc gcagccgttg tttgtgcgac
     2941 aatgtaatga ggcaaacggc aaacaaataa tggattttct cgaccccaag aagaaacgca
     3001 actataaaat ccggctgatt atcggctacg tgctggtggc tatcgcgctg gtattggcca
     3061 ccgtcgtatt ggtctatggc gcttatggct acagtatcaa caccaaaacc ggcagcgtca
     3121 tccaaaacgg cctgttgttc gtcgattcca agcccggagg cgccactatt tatcttaaca
     3181 gccagcgtca cggtacgacc tccgcccgat tagtgctgcc ctccggtaac tatactctta
     3241 aattgacgaa gaccggctac cgcaattggc agcggtctat cagcttggaa gcccagacca
     3301 tcgaccggtt cgtatatccg tttttattcc cgaccaaacc cagggtgacc aatcttaaga
     3361 cctatgtctc ggtgccgccg ctggttacag aaacgccaga taggcactgg ttgctggtac
     3421 aacttcccaa ttccgaccct tcggtcgtaa gcttcgacca atttgatact tctaaactgg
     3481 accagaccca atccccgact gtcgtcaatc tgcccgccgg tatcctgacc aataccgatt
     3541 tgccggataa ttccctgacg gaagtcgcct ggtctaccga caactcgcac ctgctgctga
     3601 agcacggcta tcagggaggc agcgagttta tcgtctttaa tcgctcagtg ccgtccgact
     3661 ccttcaacgt caataaagct ctgaaaatca accctacgga cgtagagctg cgcaatgaca
     3721 gcgtcagcca gctttatatc tacgatgccg ccgctgagtc gctcagcgtt gccgatacta
     3781 ccaaagacgt ggtggataag ccgtttttga cgcatgtgct ggcctttaag ggctatggta
     3841 cttcacttat cacctatgta acggataata agatgccagc cggccaggtc caggcgcgca
     3901 tctgggataa cggccccact tatgccctgg ctacgctggc ggcgggcgac cactatctga
     3961 tagacgccgc ccaataccag agcgactggt actacgtcgc cggcagcagc ggcaatgacc
     4021 acatcttcct ttataaaaat cccctgagca gtttgaccgc cggcgacaag gcgctgtcgt
     4081 ttatcagcct gcgggtggcc ggtgccgacc aggtcggctt ttctgccaac acccgcttcg
     4141 ttgaagcgga atccggccag cagttcggcg tttatgattt tgagaccatg acccgctatc
     4201 agtacaccgt gcaggcggcg ctggccggcc cgatgcactg gatggacggc caccgcctga
     4261 tcggcgccag caacggacaa gtctttgtaa tggactacga cggcaccaac cagcaatctt
     4321 taacaatcac cgatttcgcc ctgggcggtt atttcagcaa cgactataac catctgctga
     4381 ccacggcgcc ggccagcgac ggtacctctg ttattttgca aaacgttgac atgcgggccg
     4441 gcgccgacct gccgccggcg ccgagctccg gcagctaaaa aagattgacg attctggacc
     4501 acaaacttgg cgagtttccc gggatgaccg ccgattcagt tgccagctta ttctggccgc
     4561 tggccaccaa attgccgccg gggtcggggc tgatctgctc gccgataacc accagccggt
     4621 tgttactggt gcccggcggg tcgcaggtaa tcaagctgaa agtggcggct ttgtcggtcg
     4681 gccccaa
//
LOCUS       DASPTF010000027         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_905908, whole genome shotgun sequence.
ACCESSION   DASPTF010000027 DASPTF010000000
VERSION     DASPTF010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905908"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VEH48_00880"
     CDS             <1..261
                     /locus_tag="VEH48_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17959.1"
                     /translation="LIIATSVVYLGRWIYRSSVHTTKTPGKLKNISLKQTSQSPSGSQ
                     PAANSATNNLPNNGPGNLVAVFGVTAASAGSLHYVIKRRRGA"
     gene            complement(239..697)
                     /locus_tag="VEH48_00885"
     CDS             complement(239..697)
                     /locus_tag="VEH48_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17960.1"
                     /translation="MFGINDNRDTSYGTDTGQQSDSATAGQTYSLPPSHDDPVAMPTV
                     THQPAPASTPFPAAEPPAAAPDSSSDGGELLQIKQQALHSLTPLIDKLEQTPEEKFKT
                     TMMLLQASDNPTLVKDAYAAANQITDEKTRAQALLDVVNEINYFTHPGGA"
     gene            849..1880
                     /locus_tag="VEH48_00890"
     CDS             849..1880
                     /locus_tag="VEH48_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HXY17961.1"
                     /translation="MLDDLKYIHQRDPQDALGIAERQASQLEYKFEVPKISGDFQNIV
                     FAGMGGSALPALLSRSWPGYKIPFEVCRQYHIPAYVSDKTLFIACSYSGNTEETLSAL
                     AEAENRGAVIAVMAGGGKLEEIAGQKNYPFVKIPKAEQPRYAVFYMLVALVAVLESAG
                     LLDAAGVSEQLAQAASFLKESVKGWLPTVPQAQNPAKKLAMELAGKSGVIYAGPLLAP
                     AAYKWKIGFNENAKNVAWWDEYPEFNHNEFIGWSSHPIEKPYGVVDLRSNLEHPRVQQ
                     RFELSDRLLSGRRPAANVVQVQGSNILEQLVWAVAYGDFVTIYLALLNGVNPAPVDLV
                     EKFKQALGP"
BASE COUNT          541 a          607 c          669 g          531 t
ORIGIN      
        1 ctgattatcg ccacgtctgt ggtgtatttg ggccgctgga tttaccgctc gtcggtacac
       61 acaactaaaa cgccgggaaa gctgaaaaat atatctctta aacaaacttc gcagagccca
      121 tccggcagcc aaccggcggc caattcagca accaacaatc tgcccaacaa cgggccgggc
      181 aatttggtgg cggtatttgg cgtgacggcg gccagcgccg gcagtttaca ctacgttatt
      241 aagcgccgcc ggggtgcgtg aagtaattta tctcgttaac cacgtccagt agcgcctggg
      301 cgcgggtttt ttcatctgtt atttggttgg cggcggcgta ggcgtctttg accagcgtcg
      361 ggttgtccga agcctgcagc agcatcatgg tggtcttgaa tttttcttcg ggcgtttgtt
      421 ccagcttgtc aattaaaggc gtcaagctat gcaaggcttg ctgcttgatc tgtagtagtt
      481 cgccaccatc actgctggaa tcgggagcgg ctgccggtgg ttcggctgca ggaaaaggag
      541 tgcttgccgg tgctggctgg tgcgtgaccg tcggcatagc aactggatcg tcgtggctgg
      601 gaggcaggga ataagtctgg ccggcagtcg ctgaatctga ctgctgccca gtgtctgtgc
      661 cgtaacttgt gtctcggttg tcgtttatac caaacatacc acccttcggt ttgctaaaag
      721 caagcaatta gcaattttca atgatttaac ggttgacgtt tctcatttta aagcataagc
      781 gaagatttgg taaatgccaa atggcaaaat aaatgttcaa tgctaaatga taaatgtaaa
      841 atagggttgt gttagacgac ctgaagtata tccaccagcg cgacccccaa gacgcccttg
      901 gcatcgccga gcggcaggcc agccaactgg aatacaagtt tgaagtcccg aaaatcagcg
      961 gtgattttca gaatatcgtg ttcgccggca tgggcggatc ggccttaccg gcgttattaa
     1021 gccgcagctg gccgggctac aaaataccgt ttgaagtctg ccggcagtac catattccgg
     1081 cctacgtatc cgataaaacc ttgtttattg cctgcagcta ctccggtaat accgaagaga
     1141 ctttgtcggc tttggcggag gccgaaaata ggggtgcggt aatcgccgtg atggccggcg
     1201 gcggcaagct agaagagatt gccggccaaa aaaattatcc tttcgtaaaa atcccaaagg
     1261 ctgagcagcc gcgctatgcc gtgttttata tgctcgtcgc tctagtcgcc gttttagagt
     1321 ctgcgggatt gctagatgcc gcgggagtgt ctgaacagtt ggcccaggca gcatctttcc
     1381 tgaaagaatc ggtcaagggc tggctgccga cggtgccgca agcgcagaat ccagccaaaa
     1441 aactggctat ggagctggcc ggcaagtcgg gggtgattta cgccgggccg ctgctggcgc
     1501 cggcggctta taaatggaaa atcggcttca acgagaacgc caagaacgtc gcctggtggg
     1561 atgaatatcc ggaattcaac cacaacgaat tcatcggctg gagcagccac cccattgaga
     1621 aaccgtacgg cgtggtggac ctgcgttcca acttggagca tccgcgggta cagcagcgct
     1681 ttgaactgag cgaccgcctg ctttccggcc ggcgcccggc cgctaacgtg gtgcaagtac
     1741 aagggagcaa catcttggaa cagctggtct gggcagtcgc ttacggcgat ttcgtaacga
     1801 tttatctggc actgctcaac ggagtgaatc cagcgcccgt agatctggta gaaaaattca
     1861 agcaggcgct cggaccctaa cttttccgga cgcagcctca ccgtgagtag ccgttgttta
     1921 aaacaggctc gctgcggaac tcctcaaaaa gactgccgac tggcagtctt ttctcgttaa
     1981 acaagtcctc gcgcccaagg acgcctcttt taaacaacac ctaatcaggg ctcggcggat
     2041 gcccggaaaa gttagggcct cgctaaacag atcggtaaag atacttgact agtcaagtat
     2101 cttttgttta gtgagccctg tcgccaaaca ggctaaacct tgcccaagcg gagccgggcc
     2161 ggtcggctgt ggagatgcgg taatcattat tcgcagctca gacagccgac cgtccgcgcg
     2221 tgagcggcct gtttggcggc agggcgagcg gtcattcgcc gcgcaaagca tcaatcgggt
     2281 gtttgcgggc agctttgagg gccgggacga cgctgaagat cacacccacg atcacagata
     2341 cgccgacc
//
LOCUS       DASPTF010000028         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_39074, whole genome shotgun sequence.
ACCESSION   DASPTF010000028 DASPTF010000000
VERSION     DASPTF010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39074"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..942
                     /locus_tag="VEH48_00895"
     CDS             229..942
                     /locus_tag="VEH48_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17962.1"
                     /translation="MGVYSNTQIRQAIKDGHILCEPFNPRHVAHASLDITLGYYYYRT
                     ERVGDHTLYNPFDEYDVDRYFSGPHLAVGHREWCQENGMKPLYGVPASHPVIPIKPGE
                     RILAHSHEFFGILPPGAYELKSRSSWGRNGIAVCFDAGWVDPGYINRLTLEIYNLNQK
                     ETVLLPVGERIAQAVFHHTGPVKGTYGAGRGKGFSGKYQVGSDLKTLIKRWRPEQMLP
                     QAYKDKRSLPKPIKGLKTY"
     gene            964..2826
                     /locus_tag="VEH48_00900"
     CDS             964..2826
                     /locus_tag="VEH48_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HXY17963.1"
                     /translation="MQQYPGRFIVLEGSDGSGKTTQFNLLAERLRAVGYEVEVFDFPR
                     YDQPSSHFVRAYLNGDFGPAAKISPYAASIFYALDRYEAAPGIRRALEAGKIVLSNRY
                     VGSNMGHQGAKFSDEAEQRGFFVWNDSFEFELLGIPRPDINLYLRVPPPITLRLIKER
                     AKKTGVQPDQHEADKEHLAKAAAAYDSLCKLFPKDFVPVDCAKKGKLLTIPAINDKLW
                     GILKTILPPPPHRGRGTVVKLTNLSGPPPAKTPAAPAGEQKTQTLRLSLQSLLGLLAK
                     DQTGIRFELPESFSGKDGDYYLPAGLDSATSKQYRDGMRRLAELQRQMRSAVLEHLQK
                     RKSSASQAVMKRLADNAVLPVVPLAALSKVESALSKAELSQDGSQAVGVQQLVKERLP
                     GNLAAGGEELKLLDFWPRSEFSLLADGLYPYTDLSRQEILDGIDNWTYERKEKALRVI
                     LADRDSQVMDELKYRWDGLSAATLILKAWRRGLVKELRLQKPTVRFGYDMPALIEAAG
                     QENRYLDCFDLSLALFSSLQAAGHADDASYAALLGHKLRWQFSLNSAKLNNFGESDSD
                     YAVFIAMLKEKIAEVHPLIFQALNSRLPGVQPANPARNDDKTRQPAKKPRRRRL"
     gene            2857..3096
                     /locus_tag="VEH48_00905"
     CDS             2857..3096
                     /locus_tag="VEH48_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17964.1"
                     /translation="MAEDQFTKLFKYIESFRKDVDEKFDRVNNRLDSIEKLIDKVIKN
                     QEINDEERLVMGHQLDRLDRWVHEVADKIGYKLSI"
BASE COUNT          773 a          884 c          832 g          641 t
ORIGIN      
        1 tttagcgccg acaaattgct gcagaacatc gaggccgccc tggccagcct gcgtaccgcc
       61 cgcccggcca gcgtcaaggg tccgtacatc aaatcggtct atctgaccac caccatgggc
      121 ccgtcaatca ggctggcagt cgataactct taaaactaca acacatttcg gcttgccgcc
      181 tattgccaac ctctgtaaac tattattaaa gaggtaaaaa tcgaactaat gggggtttac
      241 agcaatacgc agatcagaca ggccatcaaa gacggccata ttctctgtga gccgttcaat
      301 cccagacacg tggcccacgc cagtttggat atcaccttag gctactacta ctatcgcacc
      361 gagcgggtcg gcgaccacac gctgtataat ccctttgacg agtacgatgt tgaccgctat
      421 ttcagcggcc cgcatctggc tgtcggtcac agagaatggt gccaggaaaa cggcatgaaa
      481 ccgctgtacg gcgtgccggc gtcccacccg gtcatcccta tcaaaccggg cgagcgcatc
      541 ctggcccaca gccacgaatt tttcggcatt ttgccgcccg gagcttacga gcttaaatcg
      601 cgctccagct ggggccggaa cggcatcgcg gtctgttttg acgccggttg ggtggatccc
      661 ggctacatca accgcctgac attagagatc tataacctca atcagaaaga aacggtactg
      721 ctgccggtag gcgagcgcat cgcccaggcc gtctttcacc acaccggccc ggtaaaaggc
      781 acttacggcg ccgggcgcgg caaaggcttc agtggtaaat accaggtggg tagtgatctt
      841 aagacattga tcaaacgctg gcgcccggaa cagatgttgc cgcaggccta caaggataag
      901 cgcagtctgc caaagccgat taagggttta aagacttatt gaggcgcagg cgggaccaca
      961 tttatgcagc aataccctgg cagattcata gttttggaag gttccgacgg ctccggtaag
     1021 accacgcagt ttaacctgct ggcagagcgt ttgcgagccg tcggctacga agttgaggtg
     1081 tttgactttc cgcgctacga ccagccctcc agccactttg tacgcgccta cctaaacggc
     1141 gatttcggtc cggcggccaa aatcagccct tacgcggcct ctatcttcta tgctttagac
     1201 cgctatgaag ccgcacctgg tatccgccgg gcgcttgagg ccggcaaaat cgtgcttagc
     1261 aaccgttacg ttggctccaa catgggtcac cagggagcca agttcagcga cgaagccgag
     1321 caacgcggct tttttgtctg gaacgacagc tttgagtttg agctgctggg tattccgcgc
     1381 cctgacatta atttgtatct gcgtgtacct cctcctataa cgctcaggct gattaaagag
     1441 cgcgccaaaa aaaccggcgt ccagcctgac cagcacgagg ccgacaagga gcatttagcc
     1501 aaagccgcgg cggcttacga tagcttatgc aaactgttcc ccaaggactt cgtgccggtt
     1561 gactgcgcca aaaagggtaa attgctgact atccccgcca tcaacgacaa gctatggggt
     1621 attttaaaaa ctattttacc gccaccaccg caccgcgggc ggggaacggt ggtcaagctc
     1681 accaatctgt cgggcccgcc gccggccaaa actccggctg caccggccgg cgaacaaaaa
     1741 acccagacac tgcgtctcag cctgcagtct ctacttggcc tgctggccaa agaccagacg
     1801 ggaatccgct ttgaactgcc ggaaagtttt agcggcaaag acggcgacta ctatctgccg
     1861 gccggtctgg attcggccac ctccaaacaa taccgcgacg gcatgcgccg cctggctgag
     1921 ctgcaaagac agatgaggag cgccgttctt gagcatttgc aaaaacgcaa aagcagtgca
     1981 tcgcaggcgg tgatgaaaag gctcgccgac aacgccgtat tgccagtcgt acctctagcc
     2041 gcgctgtcaa aagtggagtc agctttaagc aaagccgaac tttcgcaaga cggctcccag
     2101 gctgtgggcg tccagcagct ggtgaaagaa cgcctacccg gcaacttggc agccggcggc
     2161 gaggagttaa aattgctgga tttctggccg cgcagcgagt tcagcctgct ggcggacggc
     2221 ctgtatcctt acaccgacct cagccgtcag gaaatcttag atggtataga taactggacg
     2281 tatgagcgca aagagaaagc cctgcgggta atccttgctg acagggacag ccaggtcatg
     2341 gatgagctca agtaccgctg ggatggcctg agcgcggcaa ctttaatcct caaagcctgg
     2401 cggcgaggac tggtcaaaga gctaagattg caaaaaccga cggtccgttt cggctacgac
     2461 atgccggctt tgatagaggc cgccggccag gagaaccgct acctggattg cttcgacctc
     2521 agcttggcgc tattcagctc tttgcaggcc gccggccatg ccgatgatgc ctcttacgcc
     2581 gcgcttttgg gccacaaatt gcgctggcag ttcagcctaa acagtgccaa gctcaataat
     2641 tttggtgaat cagacagcga ctacgcggtc tttatcgcca tgcttaaaga gaaaatcgcc
     2701 gaggtgcatc ctctgatctt tcaggcgctc aatagccggc ttccgggtgt ccagcccgca
     2761 aaccctgcca gaaatgacga taagactcgc cagccggcca aaaaaccacg ccgccggcgg
     2821 ctgtaagccc tagccgttat ttggtataat ttatccgtgg cagaagatca attcacgaaa
     2881 ctattcaagt acattgaatc attccgtaaa gacgtagatg agaagtttga ccgagttaac
     2941 aataggctcg acagtattga aaaattgatc gataaagtta taaagaatca ggaaatcaac
     3001 gacgaagaac gattggtcat gggccatcaa ctggacaggc ttgaccgctg ggtgcacgag
     3061 gttgcagata agattggtta taaactctca atttaaatat tagcgacggg gcggcaactc
     3121 ccaaatccgg
//
LOCUS       DASPTF010000029         6388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1774575, whole genome shotgun sequence.
ACCESSION   DASPTF010000029 DASPTF010000000
VERSION     DASPTF010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774575"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /gene="rpsR"
                     /locus_tag="VEH48_00910"
     CDS             <1..185
                     /gene="rpsR"
                     /locus_tag="VEH48_00910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXY17965.1"
                     /translation="RTDVAYFDYKDCKTLQRYINQYGQIEQRKKTGLTESKQRQLARA
                     IKRARHIALLPFVANN"
     gene            189..512
                     /locus_tag="VEH48_00915"
     CDS             189..512
                     /locus_tag="VEH48_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17966.1"
                     /translation="MPPSRNRYTEIYAPLREQAVNLQNQFHDFTGGNFNDPKAHVIHH
                     EIQQLITDIDEKREPMGIDFRIKTIQNQLAESKNAPQTFMNREHYDYMHRNYEQMREG
                     LHKFE"
     gene            539..1099
                     /gene="efp"
                     /locus_tag="VEH48_00920"
     CDS             539..1099
                     /gene="efp"
                     /locus_tag="VEH48_00920"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXY17967.1"
                     /translation="MLSITSLKKGTVFALDGVPYRVLEYSQKVMGRGGSTVNVKIKSL
                     TDGKVLAKTFHGNDQVDNANINYADCQYLYKDAHQAHFMTNSNFDSIEIGLDSVKDQL
                     VFLKEGENVMIQLFDGRPIGIELPKNLPLKVTYTENAVKGDTSSAITKDAKLETGITI
                     KVPAFIKQGDIVSVDTATGEYRERQK"
     gene            1083..1583
                     /locus_tag="VEH48_00925"
     CDS             1083..1583
                     /locus_tag="VEH48_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HXY17968.1"
                     /translation="MNGKNKLDEYIYLAYLTEPREVGESTKRSPQHLTLVPPFRFGIP
                     LSELKEAVHEVAEGTSTFQIKVAKEAKFGPNHNINVFLVEPADVVKMLHFQLMYKLQS
                     AGVSLPTNYVYDAFVPHIRIRPVHKTNLAEGQLLNVDHLAIMHKNKGYRTLLAKEELR
                     SGQTKV"
     gene            1591..1911
                     /locus_tag="VEH48_00930"
     CDS             1591..1911
                     /locus_tag="VEH48_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXY17969.1"
                     /translation="MSRGGDKLASVANKLSLNFKHKTVLDVGSSTGGFTDFALKNGAK
                     KVVAVEIGTNQMHPSLRSDPRVELHEKTDIRSLRLQNAEGRMQNVAKKNLHSSFSILP
                     RILS"
     gene            1917..2192
                     /locus_tag="VEH48_00935"
     CDS             1917..2192
                     /locus_tag="VEH48_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17970.1"
                     /translation="MSFVSLREILPAVAKLSSISTQIVAMCKPQFEADDKDLNRGVIK
                     NDSIRRQILRDFEAWAKKRFAVQAKADSKLAGSKGNLERFYLLHNKT"
     gene            2447..3040
                     /locus_tag="VEH48_00940"
     CDS             2447..3040
                     /locus_tag="VEH48_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17971.1"
                     /translation="MPNLRRTSAALALCTLSAFGLTAYNNPPATAKTGAVVTAVKTSK
                     QTKQQQAEAVRWLEHRIVHFQHMAWHWKLVMGLPLTRPGRSLAGWSIQQLKQTVVWWQ
                     HQAAQAWLQATHVPHKAAWLCIHHYEGSWTDPNAPYYGGLQMNLAFQQRYGYWLYRTK
                     GTADHWTPLEQMWVAERAYKSRGFWPWPNTARYCGLL"
     gene            complement(3123..4193)
                     /gene="ychF"
                     /locus_tag="VEH48_00945"
     CDS             complement(3123..4193)
                     /gene="ychF"
                     /locus_tag="VEH48_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXY17972.1"
                     /translation="MALSIGIVGLPNVGKSTLFNALTDADALVANYPFATIEPNTGIV
                     PVPDPRLNKLAELYHTEKIIPAAVTFVDIAGLVAGASQGEGLGNQFLAHIRSCNAVCQ
                     VVRAFEDSNVESRGTNDPKQDIGIINIELALADLQTIDKRLPRLEKEAKADPKLQSVL
                     ASLNKVKDILQAGRPLAAEDIDPEHIADLFLLSAKPVIYVFNVDEKSLNDESRKQELA
                     KIVVPTQALFICAKLESELRGLDATDRQELLESYGQDESGLVQLIHAAYDTLGLQSFL
                     TAGEKEVRAWTIAKGATAPQAAGVIHGDFERGFIAAQVISYQDLIAAGSEAAARAAGK
                     VRTEGKNYIMQPDDVVEFKFNV"
     gene            complement(4245..5933)
                     /locus_tag="VEH48_00950"
     CDS             complement(4245..5933)
                     /locus_tag="VEH48_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXY17973.1"
                     /translation="MALEFAARQGGDEVTSRRFARKITRADISALLGYKRVLSVSLYD
                     PFELFVDPLSTTDQTEVGKPIFTDIGDGELGIEEAKTIRRKKAWRVPQIVEAPETDME
                     PSVLVDFYFPGTNDQKFTAIAQLAKDLEQDLPLAPRIVPNNLSRAAALLVQARTFLND
                     FKGYLLADQTINTAANALGAYMVKTAPYVEKIVSKYSKPEHSMSVSDLVAEGQIGLLK
                     GLEKYDLQTDSKNNVLIWCFNYIRGQAFDELRDSGYMIRQSRSDIEQAKALAAHSSGR
                     IKLNDKELKSANEAIARLDQRDVDYLDEPLGPDTDYTRLDLVADTYGVDDEAGDFDLP
                     DLDLESMISALSERNQKVIRMRFGFPPYNDKDGYTNSEIAQRLGLTKSRISQLLNHII
                     LPRLEGLAKRQSAEPVPMAPSTQAQPELGPSKKENLSSRELEVLKHVLDGLNNLEIAK
                     GLFVEEETVKSHLRHILAKLSAKNRTHAVRQAFELGVFLPSGGTASKKVSLPARSKEI
                     LGLVSYGLTNSEIAETLSLSKYTVNTYVRIVLEELHARNRTHAARIGFELNILE"
     gene            complement(6016..>6388)
                     /locus_tag="VEH48_00955"
     CDS             complement(6016..>6388)
                     /locus_tag="VEH48_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17974.1"
                     /translation="KHIERITKLVGLYSPPITINEMAQSAGEAGSASIGITIKGAKNK
                     LGPIIEPNVTNMVARARKTLKNQPPETLFAPTWKTIVAAETEEPDELRFQDTDRIGHS
                     VLVDDLQPELSGSARRSTVHS"
BASE COUNT         1641 a         1612 c         1525 g         1610 t
ORIGIN      
        1 accggaccga cgtagcgtat tttgactaca aggattgtaa gaccttgcag cgctacatca
       61 accaatacgg ccagatagaa cagcgcaaaa agaccggcct gaccgaaagt aaacagcgcc
      121 agttggcccg cgccatcaaa cgcgcccgcc acattgcatt gttgcccttt gtagctaaca
      181 attaagctat gcctccaagc agaaacaggt acaccgaaat ttacgccccc ctgcgcgagc
      241 aggcagtaaa tctgcagaac caattccacg atttcaccgg cggcaacttt aatgatccta
      301 aggctcacgt tatccaccac gagattcagc agctcatcac ggatatagat gagaaaaggg
      361 agccgatggg catagatttt cgtatcaaga caatccaaaa ccagctggct gaatctaaga
      421 atgctccgca gactttcatg aaccgcgagc actacgacta catgcaccgg aattatgaac
      481 agatgcgcga aggcttgcac aagttcgagt agcggaactt ggtcgggaga accaatcaat
      541 gttaagcatc accagcctta aaaaaggcac tgtttttgcc ctggacggcg taccgtaccg
      601 tgtgctggaa tacagccaaa aggtcatggg ccgcggcggt tcaaccgtta acgtcaaaat
      661 taagagtttg accgacggca aggtgctggc taaaactttt cacggtaacg accaagtgga
      721 caatgcaaac attaactatg ccgattgcca gtatttgtat aaagacgctc atcaagctca
      781 ctttatgacg aatagcaact tcgacagtat cgagattggc ctcgattcag taaaggacca
      841 gctagttttt cttaaagaag gcgagaatgt catgattcaa ctttttgatg gcagaccaat
      901 aggaatcgag ttgcctaaaa acttgccgct caaagttacc tacaccgaaa acgctgtcaa
      961 aggtgacacc agcagcgcca tcaccaaaga tgcgaaatta gaaaccggca tcaccatcaa
     1021 agtaccggct ttcatcaaac aaggtgatat tgtatccgtt gataccgcta ccggagagta
     1081 ccgtgaacgg caaaaataag ttggacgaat acatctatct tgcttactta actgagcccc
     1141 gagaggtggg cgaaagcact aaacgctcac cccaacatct gacgcttgta ccgccgtttc
     1201 gtttcggtat accactttcg gagctaaagg aggcagtgca tgaagtggcc gagggtactt
     1261 ctacgttcca gatcaaggta gccaaggaag ccaagttcgg gccaaaccat aatatcaacg
     1321 tgtttttagt cgaacccgcc gacgttgtta agatgctgca ttttcagctg atgtacaagc
     1381 tgcaatctgc cggggtttca ttacctacca attatgtcta cgacgcattc gtgccgcaca
     1441 ttagaatcag gcctgttcat aagacaaacc tggccgaagg ccagctttta aacgttgacc
     1501 acctggcgat aatgcataag aacaaaggct acaggacgct tctggccaaa gaggagctga
     1561 gaagtggaca aacgaaggtc tgaccattat gtgtcgcgcg gcggtgataa gttagcttca
     1621 gtagccaata aactaagctt gaatttcaag cataaaaccg tgctagatgt tggcagcagc
     1681 accggcggtt ttaccgactt cgccttaaaa aacggtgcca aaaaagtcgt cgccgtggag
     1741 atcggcacca accaaatgca tccgtctttg cgctccgacc cgcgcgtaga actgcacgaa
     1801 aaaacagaca tccggagcct ccggctccag aatgcagaag gcagaatgca gaatgtagcg
     1861 aagaaaaatc ttcattcgtc attctccatt cttccccgga tattatcctg atagatgtga
     1921 gttttgtctc gctgcgagaa atcctgccgg ctgtggctaa gttatcgtca ataagcaccc
     1981 agattgtcgc gatgtgtaaa ccgcagtttg aggccgacga taaagatctg aacagaggtg
     2041 taatcaaaaa tgattccatc cggcgccaga ttttgcggga ttttgaagcc tgggccaaaa
     2101 aacggttcgc agttcaggcc aaagccgaca gcaagttggc cggcagtaaa ggcaacctgg
     2161 aacggttcta tctgttgcac aacaaaactt aagatgttat tataaaaaat ggcggttgtt
     2221 agaccggtcc gaaaagtccc tctgagggag tgcgcttgaa aaaaagagtg tacgctaacc
     2281 tgagaggctc ggactttaag cctagtcgct ggggacacag cggttattaa atcacttgat
     2341 aatcactgcg tcttcagcgg ccacgatggc ttagaaactg acaaaccaca aaaacagatg
     2401 ggccagattc ggcctattgt acgttcagaa ggaggggtag cttgcgatgc ccaacctgcg
     2461 acgcacttca gccgcactgg cgctctgtac gttgagtgcc ttcggcttga cggcctacaa
     2521 caaccctccg gctaccgcga aaaccggtgc cgtggtaaca gccgtcaaga catcaaagca
     2581 aaccaagcag cagcaagccg aagccgtacg ctggctggag cacaggatcg tgcacttcca
     2641 acacatggct tggcactgga aactggtaat gggtctgccg ctaacaagac ctggccgcag
     2701 tctggccggc tggagtatcc aacagctcaa gcagacggtc gtttggtggc aacaccaagc
     2761 tgcccaggcg tggttgcaag ctacccacgt gccgcataag gcagcgtggc tgtgcatcca
     2821 tcactacgag ggcagctgga ctgatcccaa cgcaccgtac tacgggggac tccagatgaa
     2881 tctcgctttc cagcagaggt atggctactg gctgtaccgc accaaaggta cggctgatca
     2941 ttggacgccg ctggagcaga tgtgggtcgc ggaacgcgcc tacaagtccc gcggtttctg
     3001 gccgtggccg aataccgccc gctactgcgg gctgctgtaa gaggatctca gagatcgggc
     3061 gccccagcct ggaggggcca cggggcgccc gatatcagtc aggtttctaa taaccgccaa
     3121 tttcacacat tgaacttaaa ttcgaccaca tcatcaggtt gcatgatata gtttttgcct
     3181 tcggtacgga ctttgccggc ggcgcgggcc gcggcctccg aaccggcggc gattaagtct
     3241 tgataactaa taacctgggc ggcgataaaa ccgcgctcaa aatcgccgtg gattacgccg
     3301 gcggcctgcg gcgcggtggc gcctttggct attgtccagg cccggacctc tttttcgcca
     3361 gcggtcaaaa aactctgcaa acccagcgta tcgtaggctg cgtggatcag ctggacaagc
     3421 ccgctttcat cctgtccgta gctttccaga agttcttgtc tatctgtcgc atctagtccc
     3481 cgtaattcac tttccagctt ggcgcaaata aacagggctt gtgttggtac gacaattttc
     3541 gccagctctt gcttgcggct ttcatcattc aagctttttt cgtccacatt gaacacataa
     3601 attaccggct tggcggacag caaaaacaaa tcggcaatat gctccgggtc aatatcttcg
     3661 gcagccaacg gtctgccagc ctgcagtatg tctttaactt tatttaaact ggctaaaacc
     3721 gactgcaact ttgggtcggc tttggcttct ttttccaggc ggggtaggcg tttgtcgata
     3781 gtttgcaaat cggccagcgc cagctcaata ttgataatcc cgatgtcttg cttcgggtca
     3841 ttagtgcctc tactttcaac gttgctgtcc tcaaaagccc gcaccacctg gcaaacggca
     3901 ttgcagctgc ggatatgcgc taaaaactgg ttacccaggc cttcgccctg gctggcgccg
     3961 gccaccaagc cggcaatatc tacgaaagtt accgccgcgg ggatgatttt ttcagtatgg
     4021 taaagctccg ccagtttgtt taagcgaggg tcaggaaccg gcacaatgcc ggtgttcggc
     4081 tcaatcgtgg caaaagggta attagctacc agcgcgtcag cgtccgtcag cgcattaaac
     4141 aaagtagact tgccaacatt cggcaaccct acaatcccaa ttgacaaagc catgcctata
     4201 ttctaacaga ggaatagact ggaccagggg attggagccg cggactattc cagtatgttt
     4261 aattcaaacc cgatccgggc tgcatgagtt ctatttctag catgtaattc ctctaagact
     4321 attcgtacat atgtattgac ggtatatttg cttaaagaaa gggtctctgc aatttctgag
     4381 ttagttaagc cgtaggatac aaggcctaaa atttccttgg atctcgccgg caagctaacc
     4441 tttttgcttg ctgtaccgcc tgatggtaaa aatacaccca gttcaaaggc ttgtctaact
     4501 gcatgcgtcc tgttctttgc tgatagcttt gccaatatgt ggcgcaaatg actttttaca
     4561 gtttcttctt cgacaaatag gcccttagca atctctagat tattgagtcc atctaaaacg
     4621 tgtttcagaa cttcaagctc tcgacttgaa agattttctt tcttggatgg tccaagctca
     4681 ggctgggctt gagtggaagg ggccatagga acaggctcag cggattgtcg tttagctaat
     4741 ccttccaatc tgggcaatat tatatggtta agtaactggc tgatgcgcga ctttgttaga
     4801 ccaagccttt gggcaatttc actatttgta taaccatcct tatcattata gggaggaaaa
     4861 ccaaatctca ttcttataac cttttggttt ctttcagata aggctgaaat cattgactca
     4921 agatcaagat ccggcaaatc aaaatctcca gcttcgtcat ccactccata tgtatcggct
     4981 actagatcaa gcctggtata gtcagtgtcg ggacccagcg gttcatctag gtagtccacg
     5041 tccctttggt ccaagcgcgc gattgcttca tttgccgatt ttaattcttt atcattcagc
     5101 ttaattctgc cggaagaatg tgccgctaga gccttagcct gctctatatc agatctgctc
     5161 tggcgtatca tatagccact gtctcttagt tcatcaaagg cttgcccgcg tatatagttg
     5221 aagcaccata tcagtacgtt atttttagag tcagtttgaa ggtcgtattt ttctagaccc
     5281 ttaagtaagc cgatttgtcc ctcagctact aagtctgata cagacattga atgctcgggc
     5341 ttactatact tagagactat cttttctaca tagggtgcag tcttcaccat atatgcaccc
     5401 agagcgttcg ccgcagtatt gattgtttga tctgccagca ggtatccctt aaagtcattt
     5461 aagaatgtac gtgcttggac caaaagtgct gctgcccggc taaggttatt aggcacaatg
     5521 cgtggggcta ggggcaggtc ttgctcgaga tccttggcca gctgggcgat agctgtgaac
     5581 ttttggtcgt tggtgccagg aaaatagaag tctactaaca cactgggctc catatctgtt
     5641 tccggagctt ctacgatttg cggcactctc catgcttttt ttcttcgtat cgtttttgct
     5701 tcctcgattc caagctctcc atcaccaata tccgtaaata ttggtttccc gacttctgtt
     5761 tggtcggtcg ttgaaagagg atctacaaag agctcaaagg gatcgtataa tgaaacagac
     5821 agtactcttt tgtaacccag aagtgcactg atgtcagctc ttgtaatttt tctagcaaat
     5881 ctcctggaag ttacttcatc gccgccttga cgggccgcaa attccagagc cacggctcca
     5941 accgctagtc ttagcctcgc tacctcaaaa gcggtgtgag gtatataagg atcgtagttt
     6001 tgatccgcga agtttctagc tgtggacggt acttcgcctt gcactgcctg ataactccgg
     6061 ttgtagatca tccaccaaga cgctgtggcc aattcgatca gtgtcctgga atctaagctc
     6121 atcgggctcc tctgtttcag cagcaactat agttttccac gtcggagcaa ataaagtttc
     6181 aggtggctgg ttcttcaagg tcttgcgagc cctggcgacc atatttgtca catttggctc
     6241 tatgataggg ccaagcttgt tcttggcccc tttgattgta atcccaatgc tggcgctgcc
     6301 agcttcgccc gcggattgcg ccatttcgtt tatcgttata ggcggactat ataagcctac
     6361 tagtttagta attctttcaa tgtgctta
//
LOCUS       DASPTF010000030         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_480002, whole genome shotgun sequence.
ACCESSION   DASPTF010000030 DASPTF010000000
VERSION     DASPTF010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480002"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..500
                     /locus_tag="VEH48_00960"
     CDS             279..500
                     /locus_tag="VEH48_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17975.1"
                     /translation="MQPQATQEPPTQTAPEPTPPTPAPEPGPTTPPAGPPMPAAPAPA
                     GAAKSSSMKWLIVAVVVIVVAVIVWLLVK"
     gene            678..2213
                     /locus_tag="VEH48_00965"
     CDS             678..2213
                     /locus_tag="VEH48_00965"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HXY17976.1"
                     /translation="MLDLPTRQKIPIMLAVMSGMLLAALDQTIVATALPRIVSELHGL
                     KDLSWVVTAYLLTSTIAIPISGKLSDIYGRKKLLLIAIGVFVGGSALSGLSQNMTELI
                     LFRALQGVGAGMLMSNAIAVIGDLFSPAERGRWQGLIGGVFGLASVIGPLVGGYLTDH
                     ASWRWNFYINVPVGILAFFMISTLMPHIISDKKRINIDFLGAGLLAGGLSALLLAFVW
                     GGNQYAWGSAEVTGMLLVSMLLLGAFGWVEHRHAKDPILELDLFKNRSFSLSMVVVFL
                     IGMAMFGAILYIPLFAQDVLGRSATNSGVILTPMVLSLVVTSIAAGQYVSRSGRYKRI
                     VVVGMAVVTVALFWFSTMNLHTTNHQLAIRMIFAGLGLGVGLPIFNLIVQNAFDHSKI
                     GVVTASVQMFRNIGATVGVAVMGSILNNRLNHGLVHAAAISANELSNLSARNISLPVK
                     AALSSSISDVFMVSSIVVSLAFFASVFLPEIPLRSGRDHEPFLDELGKEVNAPGSGRA
                     LQR"
     gene            complement(2210..2689)
                     /locus_tag="VEH48_00970"
     CDS             complement(2210..2689)
                     /locus_tag="VEH48_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17977.1"
                     /translation="MEETATVRPTKKQKELLSFIEAFIAEHGYSPSYREIMAGTGHTS
                     VATVALHVNSLIKRGHLRKRDRSARSLEVVKPSLTAGPKIVPKQLRASEEKWLVEKVE
                     LFFKEAEHAAKPSERELDQLYVLIGALKVLGLDGAAQAFIPRLSNIKSKQKDSEYAA"
BASE COUNT          568 a          749 c          728 g          682 t
ORIGIN      
        1 aaatcaacgc cggatacttc ttagtggttc tggctgtcaa cggcggcctg ctggccgcct
       61 ggcgctaaaa acaatgaggg acaacccaag gttttccctc atcggctgtg agcaaagctc
      121 atcctcgcct acgctcggac aagcagcctg ctccttttcc tccagagaaa ccacatgaag
      181 catcaattta aaatgtcgat ttgagagtga tttctgctaa acaagcttta gcgtggtaat
      241 ataggtatat aagtatataa gtaagaggtt aaacgtttat gcaaccgcaa gccacccaag
      301 agccgccaac acaaacagcc cctgaaccaa cgccgccaac tccggcgccc gagcccgggc
      361 caaccacacc gcccgccggg ccgccaatgc cagcggcgcc tgcccccgcc ggtgcggcta
      421 aaagctcatc tatgaaatgg ctgatcgtgg ccgtagtagt gattgttgtg gccgtgatcg
      481 tctggctgct ggtcaaatag cgcggcgaaa aaacgaatct agatatttat tcctgttcat
      541 acattaaagc ggcggttccc cagttcctta gacggttata ctggctattg accccggctc
      601 agtagttaat ttagccagcc cgaggttcgt accttataat agttattaaa tcgtaataaa
      661 aatagggatc cgtttttatg ctggacttgc cgacgaggca aaaaattcca atcatgctgg
      721 ccgtgatgag cggcatgttg ctggcggcgc tggatcagac cattgtcgca accgccctgc
      781 cgcgcatcgt cagcgagttg cacggactaa aagaccttag ctgggtagtc accgcctatt
      841 tgctgacgtc taccatcgcc atcccgattt ccggcaaact gtctgatatc tatggccgca
      901 agaaactgct gttaatcgcc ataggcgtgt tcgtgggcgg ctcggcgctc agcggcctga
      961 gccagaatat gaccgagctg attcttttcc gggctcttca aggagttggc gccggcatgc
     1021 taatgagtaa tgccatagct gttattggtg atctgttctc gccggccgag cgcggtcgct
     1081 ggcagggact tatcggcggg gtattcggct tggcttcggt aatcgggccg ctggtaggag
     1141 gttacctgac cgaccacgcc tcctggcgct ggaatttcta tataaatgtc cctgtgggca
     1201 tcttagcctt tttcatgata agcaccctta tgccgcatat catctcggac aagaagcgca
     1261 taaatattga tttccttggc gctgggctgc tggcgggcgg gctttcggct ttactgctgg
     1321 cctttgtgtg gggaggcaac caatacgcct ggggttcggc agaggttacc ggcatgctgc
     1381 tggtcagtat gctgctgctg ggcgcttttg gctgggtgga acatcgccat gccaaagacc
     1441 cgattttgga acttgacctt tttaagaacc ggtctttcag cttgtcgatg gtcgtagtat
     1501 ttctgatcgg catggcgatg ttcggagcga tcttgtatat tccactattc gcccaggatg
     1561 tgctgggtcg cagcgccact aactctggcg taatcctaac tccgatggtc ttaagcctgg
     1621 tagtcaccag catcgccgcc ggccaatacg tatcacgcag cggccgctat aaacgcatag
     1681 tcgtggtagg catggccgtg gtgacggttg cgctcttttg gttcagcacc atgaacctgc
     1741 acaccaccaa ccaccagctg gccatccgaa tgatattcgc cggcctgggc ctgggtgtcg
     1801 ggctgccgat ttttaatctg atagtgcaaa acgcttttga ccacagtaaa atcggcgtgg
     1861 ttacggcctc ggtgcagatg ttccgcaata tcggcgccac cgtcggtgta gcggttatgg
     1921 gcagtatcct caacaaccgc ctaaatcacg ggcttgtgca cgccgcggca atatcggcca
     1981 acgagttatc aaaccttagt gcccggaaca tcagcctgcc ggtcaaggct gccctgtcca
     2041 gttcaatcag tgatgtgttc atggtctcca gtatcgtggt gtctttggcg ttcttcgcct
     2101 cggttttcct gccggaaata ccgctgcgtt ccggccgtga ccacgagcct tttctggacg
     2161 agctcggcaa agaggtcaac gccccgggca gcggacgggc cctgcagcgc taagcggcgt
     2221 attcgctatc tttttgtttg cttttgatat tactcagtct gggaataaac gcctgggcgg
     2281 ctccgtccaa ccccagcacc ttcaacgctc caatcagtac ataaagctgg tccagctcgc
     2341 gctccgatgg cttggcggcg tgttcggctt ctttaaaaaa cagctctact ttctctacca
     2401 gccacttttc ctcgctggcc ctcagctgtt tgggtacgat tttcggccct gccgtcaggc
     2461 tgggtttgac gacttccagc gagcgcgccg aacggtcgcg cttgcgcagg tgaccacgtt
     2521 ttatcaggct gttaacgtgc aaagctaccg tagcgaccga ggtatgaccg gtaccggcca
     2581 tgatctcgcg gtagctgggg ctgtagccat gctctgcaat aaaagcttca atgaagctca
     2641 gcaattcttt ttgcttctta gtcggtctga cagtagcggt ttcttccata aaatctaatt
     2701 taaatgatac gcgctatggc cgtgggc
//
LOCUS       DASPTF010000031         7835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_921243, whole genome shotgun sequence.
ACCESSION   DASPTF010000031 DASPTF010000000
VERSION     DASPTF010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7835)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921243"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1928
                     /gene="infB"
                     /locus_tag="VEH48_00975"
     CDS             168..1928
                     /gene="infB"
                     /locus_tag="VEH48_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXY17978.1"
                     /translation="MASTIEIDEQVTVGKLAELLSLPVSQLIAELFKNGVAATVNEKI
                     DFDTAQIITGELGLDVQLAKKQRAAPSRQKRPISDKATARPPVVAMMGHVDHGKTSLL
                     DAIRGADVVAGESGGITQHLSAYQIKHGDRYVTLLDTPGHEAFAALREHGARLTDLVV
                     IVVAADEGIKPQTVEAIRFAKQAGVKIIVAANKMDKATDADLNCLKQQLSENDLTPED
                     WGGETIVMPVSAKAKTGLNELLDMILLVADVEELKADHTGVAEGLVIESHMEKGRGPV
                     AIALVEQGLLRPGNFVAVGGTYGKIRNLEATDGRVLERAGPSMPVILTGLKALPEFGD
                     EFYEVENEKQARERAAQYNQNHTATAASAATSSSELLRIISRSHQLNELNVIIKADVQ
                     GSLTTVSDSLKTLDTEEVAVRIIASGVGTVSENDVHSAASSGAIIYGFHVNLPAHVKQ
                     LAGRDRVSIRLFDIIYELIDDAKAELEKLLSPEIIEEELGTLLVKGIFKISKTEVICG
                     GEVTKGTLKAPALARLVRDKKTIADNLEVVSLKRGPQDVTAVKTGEMCGLSFKSATRV
                     PVEENDRLELFTRQTKIRSL"
     gene            1996..3678
                     /locus_tag="VEH48_00980"
     CDS             1996..3678
                     /locus_tag="VEH48_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17979.1"
                     /translation="MPAETLQPDMASVAPELNADATNAPPAEVTSPKYRKGIWKSGRR
                     PEGQAKEEALEIIDERPGPEQKPHLVLRDPKNHGHTWFVPSNAVEVLPQESEPSPELR
                     LTESNPTPQTPQTTAEVTTVLTPESSVQPAQSEVNTEAAGQPEELEDSWTLATKTDSG
                     NYVVSRGHESKILSQAEYEAFSQPAGSTEQTTESNVNNEQRRRENELYEQWKDTNIDP
                     RVILKLKENLARKLATMGEVDASTQKVLGTYEVSGHLLSEGVPVEANGDFDTEGMKTL
                     HAMSAMMDLPDNVLQRLRAEKFGDEPYSEPEPWHKDLDSFIARMKSENTERLEQENRP
                     VRQKISEGFDIGFERATELIPDKKYLAVAKTAAKYAYYGTRYGFAKIEESVAGTIEYA
                     RSDEFSRRRRATLVWIGGMLATAGSGLVGIDRKIASDPDFMKPAEPELKTEEESAPQP
                     TAQEKQETGGDQPPRPFWEIKLPLKPDAGRERFSRMLREAYQRARHPGLARSDDFFHT
                     LTPREERELARAEKQIMARPRLLRRLLGAGMIWYGRQLYSKILNDSEGKDVS"
     gene            3710..4060
                     /locus_tag="VEH48_00985"
     CDS             3710..4060
                     /locus_tag="VEH48_00985"
                     /inference="COORDINATES: protein motif:HMM:NF039821.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HXY17980.1"
                     /translation="MFQLDDNLLKELGLGDLPAAEKNKMLAHIYETLELRVGMKLAEQ
                     MTDAQLDEFEAFIDKNDEAGALKWLETNFPHYKQVVADELEKLKTEIKAQAPAIMAAT
                     MAAIQPGAQPPPAQ"
     gene            complement(4118..5026)
                     /locus_tag="VEH48_00990"
     CDS             complement(4118..5026)
                     /locus_tag="VEH48_00990"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXY17981.1"
                     /translation="MSKLKKLVFFGNERLATSATTTAPTLRALIDAGYEIKAVIASHV
                     DGVSRQKRDLEIGLVAHSYRIPVILPGDKIDLAAKLERHRAEAAVLVAYGKIIPQEVI
                     DLFPKGIINIHPSLLPKLRGPTPVETAILDGLSETGVSLMKLTAQMDAGPVYAQKKIA
                     LSGRETKFELAEKLNQEGAKLLIECLGAIFDGSFEPAVQDESQATYSRLLTKDLGLVD
                     FKQPAELIERQVRAYAGWPKSRARLHGHEVVITKARVAQSTTDGPLVMKCQPGWLEIL
                     ELLAPSGRRMSGADFIRGYFKKGLKG"
     gene            complement(4989..5564)
                     /gene="def"
                     /locus_tag="VEH48_00995"
     CDS             complement(4989..5564)
                     /gene="def"
                     /locus_tag="VEH48_00995"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXY17982.1"
                     /translation="MATKDIIISLPNPHLYAKSRRVGLITPEIHKIIEDMRSSALDWE
                     ASREHELGVALAAIQIDQPLKIVIVRNDFENKKDHSFSVLINPEITKLEGEVEEDYEG
                     CLSVPDIYGKVPRYSKVRVRALNEDGREIRVRAEGFLARVLQHEVDHCGGKMFVDIIQ
                     NNPAAFYKLTEDGKLAKMPYEQAEKAGIFRE"
     gene            complement(5597..7540)
                     /gene="priA"
                     /locus_tag="VEH48_01000"
     CDS             complement(5597..7540)
                     /gene="priA"
                     /locus_tag="VEH48_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXY17983.1"
                     /translation="MRYYEVFVADGHYHGNTPLTYSSEETLKTRSVVTVQLRARMVTG
                     FVLREVKRPDFTAKPIKTLLSSQPLPPHCLELAKWLEDYYLCTAGEAMRQFAPSRPTI
                     RRSGREATEAETGPLQLEMDKPLTADQKKALTFIRKNTSVTTLLHGDTGTGKTRVYLE
                     LAHETLKAGKSVILLTPEIALTTQLAAAVEQQLPNPVYVLHSQLTAAQRKHIWLAILE
                     AKQPVVIIGPRSALFSPVKDLGLIVLDEAHEPAYKQEKAPRYHAARVASQLGALAGAK
                     VVLGTATPLVSDYYLAQKRGAIARMTHMAVSDVGGKVETEIVDLKERHNFSRNPYLSN
                     QLIDAITTTLSAKKQIMLYLNRRGTAHLILCRKCGWQLLCPNCDIPLVYHGDHHQVRC
                     HSCGYSAVPPLECPVCASPDIIYRSMGTKALLDNAAKLFPQFRLARFDSDNLAGERVH
                     ETYAKLRAGEIDILVGTQLLAKGFDLPKLGLVGVIAADTSMGLPDYSSEERTFQLLYQ
                     VMGRVGRGHGKGRVVLQTYEPDSPVVRSAISHDWQKFYKYLIRERQDFRWPPFAYLLQ
                     LTCRRATAEGAQKAANNLKAKLINLGLPVEIIGPTPAFYARRGKYYYYQLVAKSKNRE
                     HLVQLARNVPHDWTINLDPSDLL"
BASE COUNT         1940 a         2097 c         2152 g         1646 t
ORIGIN      
        1 aagcgctggt gcacgtttcg gaaatgagcg acgacgagag cgtggacccg gaaaaattgt
       61 tcaagctcaa cgagaagaag aagttcaaaa tcctggagat agacaccgat aaccgcaaga
      121 tcgccctatc gctaaaaaac gttaagtaag atgaaaggag caagctcatg gctagtacaa
      181 tcgagattga cgagcaggtc accgtcggca agctggccga gctgctttct ttgccggttt
      241 cccaactgat cgcagaactg ttcaaaaacg gcgtggccgc cacagtcaac gaaaaaattg
      301 attttgacac cgcccagatt attaccggtg aactgggctt ggatgtacaa ctggccaaaa
      361 aacagcgcgc cgcgcccagc cgccaaaagc ggcccattag cgataaagct acggcccggc
      421 cgccggtggt ggcgatgatg ggccacgtcg accacggtaa aacctctttg ctggacgcta
      481 tccgcggcgc cgacgtcgtg gccggcgaat ccggcggcat cacccagcac ctgtcggctt
      541 atcagatcaa acacggcgac cgatacgtga ctttactgga tacacccggc cacgaagcct
      601 tcgcggcgct gcgcgaacac ggcgcccggc tgactgatct ggtggtaatc gtagtggccg
      661 cggacgaagg cataaagccc cagaccgtag aagccatccg ttttgccaag caggccggcg
      721 ttaaaattat cgtggccgcc aacaagatgg ataaggccac cgatgccgac ctcaattgtc
      781 tcaagcagca gctgtccgaa aatgacttga cccctgaaga ttgggggggt gagacgattg
      841 tcatgccggt ctcggccaag gccaaaaccg gacttaacga acttttggat atgattctgc
      901 tggtggccga cgtagaggaa ttgaaggccg accataccgg ggtggccgag ggcttggtga
      961 ttgaatcgca catggaaaag gggcgcgggc cagtggctat cgcgctggta gagcaggggc
     1021 ttttgcggcc cggaaacttc gtagccgtgg gcggcacata tggcaaaatt cgcaacctgg
     1081 aagctaccga cggccgggtg ctggagcgtg ccggcccatc catgccggtc attctcaccg
     1141 gcttgaaagc cctgccggaa ttcggcgacg agttttatga agtggaaaat gagaaacagg
     1201 cccgtgaacg cgcggcgcag tacaaccaaa accacaccgc cactgccgcc agcgccgcca
     1261 ccagcagcag cgagctgctg cgcatcatca gccgcagtca tcagttaaat gagttgaatg
     1321 tcatcataaa ggccgatgta cagggttcgc tgaccacagt ttccgacagt ttgaaaaccc
     1381 tggacaccga ggaagtagcg gtgcggataa tcgcttccgg cgtcggtacc gtcagtgaaa
     1441 acgacgtcca cagcgccgcc agctccgggg ccataattta cggtttccac gttaacctgc
     1501 cggcgcacgt caaacagctt gccggccgcg atcgggtgtc aatcaggctg tttgacatca
     1561 tttacgagct gatagatgac gccaaggccg agctggaaaa gctgcttagc ccggaaatta
     1621 tagaggaaga gctgggcaca ctgctggtca aaggcatctt taagatcagc aaaaccgaag
     1681 ttatttgcgg tggcgaagtc accaagggga ctctgaaagc cccggcgctg gcgcggctgg
     1741 tgcgcgacaa aaagacgatt gccgataatc tggaagtggt cagcctaaag cgtggtccgc
     1801 aggatgtaac agcggtcaaa accggcgaaa tgtgcggctt gagctttaag agcgcaacgc
     1861 gcgtaccggt agaagaaaac gaccgcttag agttgttcac ccgccagact aaaatccgca
     1921 gcctttaaca ccttatgaac cccttgacat tcaattcgct ggtgctaacc tgtaaatagg
     1981 tcataaaaac aaaaaatgcc agccgaaacc ctacaacctg acatggcttc cgttgcacca
     2041 gaactaaacg cggatgcaac taatgcgccg ccggccgaag taacctctcc taaatacaga
     2101 aagggcatat ggaaaagcgg ccggcgtccg gaagggcaag ccaaagaaga agctcttgaa
     2161 ataatagatg aaagaccagg tcctgaacaa aaaccacacc ttgtattacg agatcccaag
     2221 aatcacggac atacgtggtt cgtgccaagt aatgcggtag aagttttacc acaagaatct
     2281 gaaccgtcgc ctgaattaag acttacagag tcaaatccta ccccccaaac accacaaact
     2341 acagcagaag ttaccaccgt actaacgcct gaatcctcgg ttcaaccagc tcaatccgag
     2401 gttaacacag aagccgcggg ccaaccggaa gagttggaag acagctggac gctggcgacg
     2461 aagaccgata gcggaaatta tgttgtcagc cgcggccatg aaagcaaaat tttgagccaa
     2521 gctgaatacg aagccttcag ccagccagca ggatccactg agcagacgac cgagtctaac
     2581 gtcaacaatg aacagcgccg gcgagagaat gagctttacg agcaatggaa ggatacaaac
     2641 attgaccctc gcgttatctt aaagctcaaa gaaaatctgg ccagaaagct ggcgaccatg
     2701 ggcgaagtcg atgcatcaac ccaaaaagtc ctaggaactt atgaggtctc cggccatctg
     2761 ttatccgagg gtgtgccagt ggaggctaac ggcgattttg acactgaagg catgaaaaca
     2821 ctgcacgcca tgtcggctat gatggacttg ccggacaatg tcctgcagcg gttgcgtgcc
     2881 gaaaaatttg gcgatgaacc ttacagcgag cccgaaccct ggcacaaaga tctggatagc
     2941 ttcattgcca gaatgaagag cgaaaacacc gagcgcttgg agcaagaaaa ccggccggtc
     3001 cgccaaaaaa taagtgaagg ttttgacatt ggtttcgaac gggccacgga actcattccc
     3061 gacaagaagt atctagcagt cgccaaaaca gccgctaaat acgcctatta tggtacgagg
     3121 tatggctttg cgaagattga agaaagtgtc gccggcacaa tagaatacgc gcgaagcgat
     3181 gaattcagcc gacggcgtcg ggcaactctt gtctggatag gtgggatgtt agcgaccgca
     3241 ggcagcgggc tggtcggtat tgatagaaag atagcttccg accctgattt tatgaaaccg
     3301 gccgaaccgg agctcaagac cgaagaagaa tcagcaccgc agccgacggc gcaagagaaa
     3361 caagagactg gaggagacca accgcccaga cctttctggg aaatcaagtt accgctcaag
     3421 cccgatgccg gtcgggaaag gttcagtcga atgttgcgcg aagcttacca aagggcaaga
     3481 catcccgggc tggcccgtag cgacgacttc ttccacacac ttacgcccag agaggaacgc
     3541 gaactagccc gcgccgaaaa acaaataatg gcccggccgc gtctgctacg gcggcttttg
     3601 ggcgctggga tgatctggta cggcagacag ctgtactcca aaatcctaaa tgacagcgag
     3661 ggaaaggacg tatcataaaa gcctgtggta tattattctt aagaggcata tgtttcagct
     3721 agatgacaat ttgctaaaag aactcggctt gggcgacctg ccggccgctg agaaaaacaa
     3781 gatgctggcg cacatctacg agacgctgga gctgcgcgtc ggcatgaaac tggccgaaca
     3841 gatgacagac gcccaactgg atgaatttga agctttcata gataagaatg atgaagccgg
     3901 ggcgcttaaa tggctggaaa ccaactttcc gcactataag caggtagttg ccgacgaatt
     3961 ggaaaagcta aaaaccgaaa ttaaagccca ggcgccggct atcatggctg cgaccatggc
     4021 cgcgattcaa cccggtgccc agccgccgcc agcgcagtag ggtcataggg gtgtagggtt
     4081 atagggttgt agaaacaccg tttaaccctc ttacccccta acccttcaac cctttcttga
     4141 agtaacctct tatgaagtca gcgccgctca tgcggcggcc gctgggcgcc aatagttcca
     4201 ggatttccag ccagccgggc tggcatttca taaccaacgg gccatcggtg gtagattgtg
     4261 cgacccgggc ttttgttatg acaacctcgt gcccgtgcag ccgggcccgg cttttaggcc
     4321 agccggcata agcccgcacc tgccgctcaa tgagttcagc cggctgctta aaatcaacta
     4381 ggcccaagtc ttttgtgagc aggcggctgt aggtcgcctg gctttcgtcc tgtacagccg
     4441 gctcaaacga gccgtcaaaa attgcgccca aacactcaat cagcaatttt gccccctctt
     4501 gatttagctt ttcggctagc tcaaacttgg tttctctacc gcttaaagcg atttttttct
     4561 gggcgtaaac cggcccggcg tccatttggg ctgtaagttt catcagcgaa acgccggttt
     4621 cggacaaacc atctaaaatg gctgtttcaa caggggtggg gccacgtagt tttggtagaa
     4681 gggaaggatg aatgttaatg attccttttg gaaagaggtc tataacctct tgagggataa
     4741 ttttgccata agccaccaaa accgccgctt cggcgcggtg gcgctccagc tttgcggcta
     4801 aatcgatttt atctccgggc agtatgacag gaatgcggta ggaatgggcc accagcccga
     4861 tttccaagtc ccgcttttgg cgggaaacgc cgtcaacgtg gctggctatt accgccttaa
     4921 tttcatagcc ggcgtctatc aatgcccgca aggttggcgc ggtcgttgta gcggaggtcg
     4981 ctagacgctc attcccgaaa aataccagct ttttcagctt gctcataagg cattttggcc
     5041 agtttgccgt cctcggttag tttgtaaaaa gccgcagggt tattttggat gatatcaacg
     5101 aacatcttac cgccgcagtg gtcgacctcg tgttgcagca cccgcgccaa aaagccctcg
     5161 gcccgcaccc gaatttcccg gccgtcttcg ttcagcgccc gcacccggac tttactgtag
     5221 cgcggtacct taccgtagat gtccggcaca ctcaaacaac cttcgtaatc ctcttcaacc
     5281 tcgccctcca gtttggtgat ttccgggttg atcagcacgc tgaaactgtg gtcttttttg
     5341 ttctcaaaat cgttgcggac gatgacgatt ttcagcggct ggtcaatctg gatggccgcc
     5401 aaagccacgc ccagctcgtg ctcgcggctg gcttcccagt ccagcgccga cgagcgcata
     5461 tcttctataa ttttatggat ttccggagta atcagaccta cccgccgcga cttggcatac
     5521 aaatgggggt ttggcaggga aataattata tctttagtag ccatgataat agggtatagg
     5581 gtttaaggga cagggtctag agtaaatctg acggatctaa atttatcgtc caatcgtgag
     5641 gtacgttcct ggcaagttgc actaagtgct cgcggttttt gcttttggcc accagctgat
     5701 agtagtaata cttccctcgg cgggcataaa aagccggggt agggccgatg atttccaccg
     5761 gcaggcccaa attgataagc ttcgccttga gattattagc ggccttttgg gcgccctccg
     5821 cggtggcccg gcggcatgtc agctgtaaca gataggcgaa cggcggccag cggaagtcct
     5881 ggcgctcgcg tatcaggtat ttataaaact tctgccagtc gtgactgatg gccgagcgca
     5941 caaccgggct atccggctcg taagtctgca gtaccacccg gcccttgccg tggccgcggc
     6001 caaccctccc catcacctgg taaagcagct ggaaggtccg ctcctcggat gaataatcgg
     6061 gcaggcccat agaggtatcg gcggcaatta ctcctaccag ccccagtttg ggcaagtcaa
     6121 agcctttggc cagcagctgg gtgcctacta ggatgtcaat ctcgccggct ctcagcttgg
     6181 cgtaagtctc atggacccgc tcaccagcca ggttatcgct gtcaaaacgt gccaggcgga
     6241 actgcgggaa cagtttggcg gcgttgtcca gcagtgcctt ggtgcccatg ctcctataga
     6301 tgatgtcagg gctggcgcaa accggacact ccagcggcgg cacggcgctg tagccgcagc
     6361 tgtggcagcg tacttggtgg tggtcgccat ggtagaccag cgggatatca cagtttgggc
     6421 acagcagctg ccagccgcat ttcctgcaca aaatcaaatg agccgtgccg cggcggttta
     6481 gatacaacat aatctgtttt ttggccgata acgttgtggt aatagcatca atcaattgat
     6541 ttgataagta tgggttgcgc gagaaattat ggcgctcttt aaggtccaca atctcagtct
     6601 caaccttacc gcccacatcg ctcactgcca tatgggtcat acgggctata gccccgcgtt
     6661 tttgggccag ataataatcg gacaccagag gtgtagcggt gcccagcacc actttggcgc
     6721 cagccaaagc ccctagctga ctggccacac gcgccgcgtg gtagcgcggc gctttttcct
     6781 gcttgtaggc tggttcgtgg gcctcgtcca gcactatcaa cccgaggtct ttaacagggg
     6841 aaaataacgc cgagcgcggg ccgattatga ctaccggctg tttggcctcc aaaatcgcca
     6901 gccagatgtg tttgcgctgg gcagcagtta gttgagaatg cagcacgtag accgggttgg
     6961 gcagctgctg ctccacggcg gccgctaact gcgtggtcag ggcgatttcc ggggtcagca
     7021 gtatcaccga cttgccggct ttgagggtct cgtgggccag ctctaaataa acccgggttt
     7081 tacccgtgcc ggtgtcgccg tgcagcaggg tagtaacaga ggtgtttttg cgtataaaag
     7141 tcagcgcctt tttttggtcg gcggtcagcg gtttgtccat ctccagctgt agcggtccgg
     7201 tctcggcctc agtcgcttcg cggccgctgc ggcggatggt cggccggctg ggggcaaatt
     7261 ggcgcatggc ctcgccggcc gtgcacaggt aataatcttc cagccacttc gccagttcca
     7321 ggcagtgggg cggtagcggc tggctgctta gcagggtttt gatgggcttg gccgtaaaat
     7381 ccggcctttt aacctctctc agcacaaaac cggttaccat ccgggctcgc agctgcacgg
     7441 ttaccacgct gcgggtcttc aaagtctctt ccgatgaata ggtcagaggc gtgttgccat
     7501 gatagtgccc gtcagcgaca aaaacctcgt agtatctcac cccctaattg tatctcacca
     7561 agagacctag cagagacagg gaatgacctg cctgttcgca gctgaagccc ggtttttaat
     7621 aacgtttccg cactcgcact atttacatat aagaataaat gtgctaaagt cgcattatgt
     7681 caaaccattt ggagcgtttt aacaaggtct atgaagaagt tagcagcttt ccccgtcccg
     7741 tacgtgtagg tgatacaatc tatgatggca agattgaatg cgatagctca cttatcgcaa
     7801 aggaagaaga gctcggcaag gtcgtcggcc gattt
//
LOCUS       DASPTF010000032         6548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1201147, whole genome shotgun sequence.
ACCESSION   DASPTF010000032 DASPTF010000000
VERSION     DASPTF010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201147"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VEH48_01005"
     CDS             complement(<1..338)
                     /locus_tag="VEH48_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXY17984.1"
                     /translation="MGQALYRKYRSRSFDEVIGQEHVTTTLKNALKNGRINHAYLFSG
                     PHGVGKTSVARILAHEVNKLPYENDSIHLDIIEIDAASNRRIDEIRELRERVHIAPTS
                     ARYKVYIIDEV"
     gene            complement(398..910)
                     /locus_tag="VEH48_01010"
     CDS             complement(398..910)
                     /locus_tag="VEH48_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17985.1"
                     /translation="MADIPEKQIKRLKALISEAETSLAAAKELIVSLVGEDTLLADKV
                     KDKALGKVIEGVFDGQNMVGSDGKTYPVPANYASKSKLVQGDILKLTIADDGAFMYKQ
                     IGPIPRKQLVGVLNQKDGHYFVEVNGKEYRVLLASVTYFKAKPGDQVSVNIPEDESVD
                     AEWAALEAAL"
     gene            complement(1040..1600)
                     /gene="rplL"
                     /locus_tag="VEH48_01015"
     CDS             complement(1040..1600)
                     /gene="rplL"
                     /locus_tag="VEH48_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HXY17986.1"
                     /translation="MADSEEDQVKTETAEAPAEAKEEPNEEVSKEEPKAEPKEEPKAE
                     AKEEPKAEKKADKVEVPKEFASLVEHLDKLSALEISKFVKFLEEHWGVSAAAPAVAVA
                     AAPAGGEGAAAAEAKSEYDVMLKDPGAQKVAVIKAVKDITGLGLGEAKAIVDSAPKVV
                     LEKASKDDAEAAKKTLEEAGATVELA"
     gene            complement(1624..2154)
                     /gene="rplJ"
                     /locus_tag="VEH48_01020"
     CDS             complement(1624..2154)
                     /gene="rplJ"
                     /locus_tag="VEH48_01020"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HXY17987.1"
                     /translation="MALSKDKKAEVVAEVAQLLKDSKLTVLARYPGTSVQAMQQLRRD
                     ARANGTTVKIVKNRLFKKALESSDIYKDLDKDQIQGQLMYAFNAQDEVMPAQDLAAFA
                     KSQPQIQFVGAITAEGRYLAAEDVQALADLPSKQQLRAMAVAVIAAPISGLVNVMSGN
                     VRGVLNVLSARAETLK"
     misc_feature    complement(2201..2315)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(2350..2931)
                     /locus_tag="VEH48_01025"
     CDS             complement(2350..2931)
                     /locus_tag="VEH48_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXY17988.1"
                     /translation="MIDPHEQEVTQSAPRDNPDTVFYLGRLSSKNIVELNVPYIISPD
                     GAIGVAASQVRKISAEIEADEILWVRSRGDTSEEDEESHGEIKPTELADGRIVIDHEK
                     KEVYVEGKNIHLQPKHYLVLELLATRPGFVFSRKQILEDCWGDSGFRSGATVNVAISN
                     VRKALGEDLARVIGTKRGFGFYVQESFRQTSAE"
     gene            complement(3269..3739)
                     /locus_tag="VEH48_01030"
     CDS             complement(3269..3739)
                     /locus_tag="VEH48_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HXY17989.1"
                     /translation="MPGNPRAISIKISGSGKARIGEVTQVVLNDARLSSRVRDLIRAA
                     GNYDRPISEATKVLESRIRKKANPPNKKMSGDALVAYAFNEDVTKTRLKMSDDPDEQR
                     GYTQIMRGVVAAFRNPTHHDIVDTFSQEDALKICAFIDLMLPIVDNAEDVNSSP"
     gene            complement(3978..4166)
                     /locus_tag="VEH48_01035"
     CDS             complement(3978..4166)
                     /locus_tag="VEH48_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17990.1"
                     /translation="MAEIQEEQSIIWLLRDLMTVELAKACCTNEEIRKIVKVQNNRES
                     GIAGPVRKAIKRSGQPEA"
     gene            complement(4159..4377)
                     /locus_tag="VEH48_01040"
     CDS             complement(4159..4377)
                     /locus_tag="VEH48_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17991.1"
                     /translation="MNEEILKELRSIKNLLAVEAIKGLSRKEQILILNGAGFDNKSVA
                     QMVGTTPGTVAVAVSQEKSKRRSGKKNG"
     gene            complement(4777..5538)
                     /gene="heR"
                     /locus_tag="VEH48_01045"
     CDS             complement(4777..5538)
                     /gene="heR"
                     /locus_tag="VEH48_01045"
                     /inference="COORDINATES: protein motif:HMM:NF037099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HXY17992.1"
                     /translation="MSNIFMAGLRRLNAIAGLLHLAQGIAVLILSTNFTLPITGGYLK
                     LNPATNHLEAGSNTLFHLSLPWLVAIFFFLSAVFHLAIATFYNRTYVANLARGINRAR
                     WFEYSLSASVMMVAIAMLTGIYDAMSLLMIFALVAIMNLTGLAMEVYNQGRQNINWLA
                     YKIGSLAGFVPWVAVAFYIANSSHVGSGAPTFVYFIFGSIFIFFSCFALNMYLQYKKV
                     GKWADYLYGERMYIILSLVAKSLLAWQVFAGTLRP"
     gene            complement(5597..6367)
                     /locus_tag="VEH48_01050"
     CDS             complement(5597..6367)
                     /locus_tag="VEH48_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17993.1"
                     /translation="MIELAPLFDLTAFHEGGRRSMELVRLPKTPEVTPDMWDIAVLPA
                     GENEPVYDALEDAYLPRAETLERRGRLQEAADAYIDFANQLQNAGFLLKAATVKVKAA
                     ELYEKLGGYEVAELELIDVTETLLRCARILKDHSRDLGFETNIRATYPNVFTFMPYSK
                     NGQVFPDFHDANLEKYTLEQYVTLCRVMHKAGHPDLAMTAMGICGTLSEEFKRKHYDP
                     KYVQNYSFDEYRTAAYHSAIGHYGPFEDGNLRYKITTH"
BASE COUNT         1493 a         1694 c         1468 g         1893 t
ORIGIN      
        1 acctcgtcaa taatgtagac cttgtatctg gctgaggtcg gcgcgatgtg tacccgttcg
       61 cgcaactcgc ggatctcgtc tatccggcgg ttgctggcgg cgtcaatctc aatgatgtcc
      121 aggtgtatgc tgtcattttc atagggtaat ttgttgacct cgtgcgctaa aatccgcgcc
      181 acactggtct taccgacgcc gtgcggcccg ctgaacagat aagcgtggtt gatgcgtccg
      241 tttttgagag cgttttttaa cgtcgttgtc acatgctcct gaccaatgac ctcatcaaac
      301 gaccttgaac gatatttccg atataaagcc tgccccatat acatttcatt ataatgaaat
      361 gtaccagctt atagctacga gcttttagct gttagcttta caacgcggct tccagggcgg
      421 cccattcggc gtccaccgat tcgtcctcag gtatgttcac actgacctgg tcgcccggct
      481 tggctttaaa gtaggtcaca ctagccagca gtacgcggta ttccttgccg ttgacttcca
      541 cgaaataatg tccatctttt tggttgagca cacccaccag ctgcttgcgc ggaatcggtc
      601 cgatttgctt atacataaag gcgccgtcat cggcgattgt cagttttaag atgtcgccct
      661 gcaccagctt ggacttagaa gcgtaattgg ccggtaccgg gtaagttttg ccatcggagc
      721 cgaccatatt ctggccgtca aaaactcctt ctatgacctt gcccaaagcc ttgtctttga
      781 ctttatcagc caacagcgtg tcttcaccca ccaggctgac aattaactct ttagcggccg
      841 ccaaactggt ctccgcttcg cttatcaacg cctttagccg tttgatttgt ttttccggga
      901 tatccgccat tttgtttctc caaaacccac tcgcctcgta aaaaaatctt tgtttgactc
      961 ttccctaggc tcgttctagc cccaaaacta acatagtctg gggcttaagt caaagctgct
     1021 gtggataagt aaccgctgct taggccagct caactgtcgc gccggcttct tccagagttt
     1081 tcttggcggc ttcggcgtca tctttgctgg ccttttcaag taccactttc ggcgcactgt
     1141 ctactatggc tttggcctca ccaagaccca ggccggtaat gtcttttacg gctttgatga
     1201 cggccacttt ttgggcgccg gggtctttta gcatgacgtc gtattcgctt ttggcttcag
     1261 cggcggctgc gccttctcca ccagccggcg cggcggctac ggctactgcc ggagcggcag
     1321 ccgaaacacc ccagtgttcc tctagaaatt taacgaattt gctgatttcc agggcgctga
     1381 gtttatccag atgctccacc aggctggcga actctttggg cacttctacc ttgtcagctt
     1441 ttttctcagc cttcggctct tctttggcct cggcctttgg ctcctctttg ggttccgctt
     1501 tcggctcttc tttactgact tcttcgtttg gctcttcttt ggcctcggcc ggcgcttctg
     1561 ccgtctcggt ctttacttgg tcttcttcgc tgtctgccat cttgttctcc tttacttaat
     1621 tgattacttg agcgtttcgg ccctggcact taaaacgttc aaaactccgc ggacgttgcc
     1681 gctcatgacg ttgaccaggc cgctgattgg cgcggcaata accgccactg ccatggcccg
     1741 caactgttgt ttgctgggca gatcggccag ggcttgcacg tcctcggccg ccagatagcg
     1801 gccttcggcg gtaatcgcgc ccacaaactg gatttgcggc tggcttttgg caaaagccgc
     1861 cagatcctgg gccggcatta cctcgtcctg ggcgttgaaa gcgtacatca gctgcccctg
     1921 gatctggtct ttgtccaggt ctttgtagat gtcgctggac tccagagctt tcttaaaaag
     1981 ccggtttttt acgattttta cagttgtgcc gttggcccgg gcatcgcgcc tcagctgctg
     2041 catcgcctgc accgacgtgc ccgggtagcg ggccagaaca gtcagcttgg aatctttcaa
     2101 aagctgggca acttcggcta caacttcggc tttcttatct ttggataatg ccatagataa
     2161 ttctccttgt tttaacaata tcgaccaatt tgttaagttg tcttgagcta aaaaagtctt
     2221 tggactttat ccaaagacat taagagagtt ttgcaacctc ggcgacatga ttaaggtttc
     2281 cccgtcgctg tctttggctc aagttcaaga ggaatattag cacagatagc gaactctgac
     2341 aagcagatct tactcggctg aggtttgccg aaaactctct tgaacataaa agccaaaccc
     2401 acgctttgtt ccaataacgc gggctaagtc ctcccctaat gcttttcgaa cgttcgatat
     2461 tgctacattc accgtcgcgc cacttcggaa cccagaatct ccccagcaat cttccagtat
     2521 ttgctttctc gaaaaaacaa aacccggtcg ggtggcaagc agttcgagta ctaggtagtg
     2581 ctttggttgc agatgtatgt tttttccttc tacatacact tccttttttt catgatcaat
     2641 cactatacgt ccgtccgcca actcagttgg ttttatctcg ccatggctct cctcgtcttc
     2701 ttcggaagtg tctccgcggc tccggaccca taggatctca tcggcctcga tttcggcaga
     2761 tattttcctt acttgagaag ctgctacccc tattgcgccg tccggagaga ttatgtacgg
     2821 cacgttcagt tcgactatat ttttactact aagtcttcca agataaaaaa ccgtatcggg
     2881 attgtcccgc ggcgctgatt gagttacttc ttgctcatgg gggtcgatca tgcagcatac
     2941 tataccatat tttgttccat tttgtgccgg caacacggaa cccaaaacct ctcggcactt
     3001 tctcgccctg acacattgct gtcgcaatca ggctcgcaaa agaacttgag acaaaacatg
     3061 cccccagcat tttttaactc ttcggacatg cttttgcgcc caagggtgcc taattccaca
     3121 acatcaaatc agggctcgaa agatgccgaa ctaattcagt aaatctagat aaaatcaaaa
     3181 aagcacctcg tttgaagtgc atatctagtc gctgtctttg gctcaagttc caaactgtat
     3241 tatcaaaact ctatccgtta atcaatggct atggggatga gttcacatcc tctgcgttat
     3301 cgacaatcgg tagcatcaag tctatgaaag cgcaaatctt taaagcatcc tcctgggaga
     3361 atgtatcaac tatatcgtgg tgagtcggat ttcgaaatgc cgccaccact ccgcgcatta
     3421 tttgcgtata acctcgctgt tcatctgggt cgtcactcat cttgagacga gtttttgtca
     3481 catcctcgtt aaatgcatat gctaccaatg cgtcccctga catttttttg ttcggaggat
     3541 tagctttttt tctgatgcga ctttctaata ctttagtggc ttcactaatt ggacggtcgt
     3601 agttcccggc ggccctgata agatctcgta cacggctgct gagccgagca tcattaagta
     3661 caacctgggt gacttcacct atcctagctt tacccgatcc ggatatttta atgctaatag
     3721 cgcggggatt accgggcacg tcttgatgat atgttgatga tgaggacaac gctgctctga
     3781 ttacttcggt acgctgatgg ctgtctaacc tcctaagagc tgactgatag ccaataccga
     3841 gatctcgaga tagaattaca agagccgctg ggctcgaaat tccagtagac ttcgcgagac
     3901 gcaaaattcg attccttaca tattgaagat cgccaggacg acccgacttc tgagctactt
     3961 tattgagaag attctggtca agcttcgggt tggccactac gctttattgc ctttctgacc
     4021 ggccccgcaa taccgctttc tctattgttt tgaaccttaa cgatttttcg gatttcctcg
     4081 ttagtacagc aggctttagc caattcaacc gtcattaaat cgcgcagtag ccatattatc
     4141 gattgctcct cctgaatctc agccattttt cttacccgac ctcctcttac tcttctcttg
     4201 agagacagct actgccacag tacctggtgt tgtgcctacc atttgggcaa cagacttatt
     4261 atcgaagccc gctccattca gaatcagtat ttgttccttg cgagatagcc ccttaattgc
     4321 ttcgacggct agcaaattct ttattgagcg cagttctttt agaatctctt cgttcacagt
     4381 tttctcctct tctttccttt cctttttacg tacagcgtct gactaactac agccggtgtt
     4441 gccccagtaa gatcagcaat tcgtttcgcc gatatgccta cgctgtccag ttcgactatt
     4501 agatcgcttt gagttttttg ctgcctaagc aaaagggaaa ttaaccgtga aagctcatcc
     4561 gttgaccctg cttctacgcc cccattactc ataacatatt agtataagca cgttcgtaat
     4621 taacgtcaac tgctaagttt aggctactct gttaattttg cttatgctta ttgatatttt
     4681 taatttgtta aattatttga cagatagagt agttttggaa atctatgctt tgattaaaca
     4741 ttaggatttt tggggttgcc accccgccat tttaggtcag ggacgcaggg tcccggcaaa
     4801 gacctgccag gctaggagtg atttggctac gagactgaga ataatgtaca ttctttcgcc
     4861 gtaaagataa tcggcccact tgccaacttt tttgtactgc aggtacatat taagtgcaaa
     4921 acagctaaag aaaataaaga tactgccgaa tataaagtaa acaaacgttg gcgcgccgct
     4981 gccgacgtgg ctgctattgg ctatgtaaaa cgccacggct acccaaggga caaagccggc
     5041 caacgagccg attttatacg ccagccagtt aatattttgc ctgccttggt tgtaaacctc
     5101 catggccagg ccggttaaat tcataatggc gaccagcgcg aagatcatca gcaggctcat
     5161 ggcgtcgtaa atgcctgtta gcatggctat agctaccatc atgacgctgg cgctcaaact
     5221 atattcaaac cagcgtgccc ggttgatgcc gcgggccagg ttggcaacat aagtacggtt
     5281 ataaaaggta gcgatggcca gatgaaaaac ggcgcttaag aagaagaaaa ttgctaccag
     5341 ccacggcaaa gacagatgaa aaagcgtgtt actgcccgcc tctaggtggt ttgtggcggg
     5401 gtttagctta aggtaaccgc cggttatggg caaagtgaag ttggttgaaa gaattaggac
     5461 ggctatgcct tgagccaaat gcagcagccc tgcgatcgcg ttcagccgcc gcagaccagc
     5521 catgaatata ttgctcatgc ttaaaagtat actccgccgc agtaaaattc aggatttttt
     5581 ggtttgccgc cccgcgctag tgagtggtga ttttataacg gagattaccg tcttcgaatg
     5641 gtccgtagtg acctatggcc gaatggtaag ctgctgtgcg gtattcatcg aagctatagt
     5701 tctgtacgta tttaggatca tagtgtttac gcttgaactc ctcgctcaag gtaccacaga
     5761 ttcccatggc agtcatggcc aaatcgggat ggccggcttt atgcatgact cggcagagcg
     5821 tgacatactg ttccagagta tatttttcca gattggcatc atggaaatct gggaaaactt
     5881 gtccattttt agaatacggc ataaaggtaa aaacattcgg ataggtggcg cggatatttg
     5941 tttcaaagcc gagatcgcgc gagtggtctt tcaatatacg agcgcaccgt agaagtgtct
     6001 cagtaacgtc tatgagctca agctccgcta cttcgtagcc cccgagtttc tcataaagtt
     6061 ctgctgcttt cacttttacg gttgcggctt tgagcagaaa tccggcattc tgcagctggt
     6121 tggcaaaatc tatataggca tcagcggctt cctggagcct acctcgccgc tctaaagtct
     6181 cggcacgagg caaataggcg tcttccaacg cgtcataaac gggttcattc tcgccggcag
     6241 gcaatacagc tatatcccac atgtcgggtg taacttccgg agtctttggt agcctaacca
     6301 gctccataga tcgtctgccg ccttcatgaa aggcggttaa atcaaacaga ggtgctagct
     6361 caatcataat ctattattta tacaacaaaa ccttaaataa ttcaataccg gatttgggag
     6421 ttgccgcccc gtcgctaata tttaaattga gagtttataa ccaatcttat ctgcaacctc
     6481 gtgcacccag cggtcaagcc tgtccagttg atggcccatg accaatcgtt cttcgtcgtt
     6541 gatttcct
//
LOCUS       DASPTF010000033         4146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1201320, whole genome shotgun sequence.
ACCESSION   DASPTF010000033 DASPTF010000000
VERSION     DASPTF010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201320"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VEH48_01055"
     CDS             <1..190
                     /locus_tag="VEH48_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY17994.1"
                     /translation="LFVMLIAMSKGYVYLWIFIGSSVGSYLPVLFHQGFFSASSIIAG
                     AVGAFAGIWIAVKTQDMF"
     gene            213..289
                     /locus_tag="VEH48_01060"
     tRNA            213..289
                     /locus_tag="VEH48_01060"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:247..249,aa:Val,seq:cac)
     gene            296..610
                     /locus_tag="VEH48_01065"
     CDS             296..610
                     /locus_tag="VEH48_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HXY17995.1"
                     /translation="MKKLPDKPTLADYQTYLKEVNTERGFKETVQDVFVLLVEEVGEF
                     AKAIRKRGVIKVDKNSKEHNVEEEAADVFYLLINLCNHLNIDLEKAFRNKEKKNQKRH
                     WS"
     gene            607..1113
                     /locus_tag="VEH48_01070"
     CDS             607..1113
                     /locus_tag="VEH48_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXY17996.1"
                     /translation="MKKHSAGLVVYRKKAGQLEVLLAHMGTPWWAKKDKGAWTIPKGE
                     YQENEDALAAAKREFKEELSHEVPDGKLLELGSIEQNNNKTVVAWALEADLDVSHISS
                     NTTTIEWPPRSGRTQEFPEIDRAAYWSLPEAAARIVPGQTGLLERLAEKLAVPFDPAA
                     EPKQGSLF"
     gene            1147..2592
                     /gene="metG"
                     /locus_tag="VEH48_01075"
     CDS             1147..2592
                     /gene="metG"
                     /locus_tag="VEH48_01075"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HXY17997.1"
                     /translation="MGKFYVTTAIPYVNDKPHLGHAMLHLYADVLARYHRQIGDKVLF
                     AAGTDEHGGKIAEKAAEAKLTPQQFADQVSRSFIALLKELNISNDRFIRTTDASHEHR
                     AQLIWKNLAKYIYKGTYEGWYCTGDEEFFSEQTVKANNGVCPDHNRPYEKLREENYLF
                     ALSKFTTQIKEAIETDAFKVIPDSRKHEILSLLKSGLEDISVSRPKDKISWGVPVPGD
                     KDQVMYVWFEALMNYITVLGYPDQPDFKLFWPADVQVIGKNILRFHAAIWPAMLIGLG
                     LPLPKQLYAHGFIEVDGKKMSKSLGNVVDPLDVIAKYGTDTFRYFFLRHISSYEDGDF
                     SWARLEAAYNDELADQLGNAVGRVAAMIKNYQKGLIGDIPAPDHDIAPFQQALADCRF
                     DRAVEEIWDQVKGLNQYIDETKPWEIAKAGDADHLREVLAYAAASLIEIATLLTPFLP
                     ETSANILAVFNTGVLKPLPGPLFPKHETKTG"
     gene            2602..3387
                     /locus_tag="VEH48_01080"
     CDS             2602..3387
                     /locus_tag="VEH48_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXY17998.1"
                     /translation="MELIDTHCHLHFDDYAADRQAVFQASAAAGVKRLICVGVSLADS
                     RRAIDLAQKQPGVWATAGAHPHDGADFLADGQAPAKLAAMLKEPKVVAVGEIGLDYYR
                     DITLKADQEKVLRTQLDVGAPSGLPFVFHVREAWDGFWPVFDSYRGITGVIHSFSAGP
                     EQLEQALSRGLHIALNGLMTYTKEASWLAAAKRVPLDRLVLETDAPFLTPKPYRGQRC
                     EPKHTADTCRFLAELRNEDPAVLAAATTANAVKLFRLGEERIG"
     gene            3384..>4146
                     /locus_tag="VEH48_01085"
     CDS             3384..>4146
                     /locus_tag="VEH48_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXY17999.1"
                     /translation="MKSIYLDYAAATPVDEKVIAAMQPFFSDQFYNPSATYLAGRAAR
                     RALDEARSSVAGWLGARPGEITFTAGATEANNLVISGLMRHFPDGEVLVSAIEHESVL
                     APASQFQCRQIPVTRQGITDLAKLEKMITPKTVLVSVMLVNNELGTVQPVKEVAKILN
                     KSSILRKGGLPLYLHSDAAQAASLFDLHVSRLGVDMLSFNGGKIYGPKQSGALYVRAG
                     IELQPLILGGGQERNLRSGTENLAAAAGLAKALELA"
BASE COUNT          999 a         1123 c         1171 g          853 t
ORIGIN      
        1 attatttgtt atgcttattg ctatgagcaa aggctacgtt tatctttgga tttttatcgg
       61 cagcagcgtc ggctcgtatt tgcccgtctt attccaccaa ggattttttt cggcctccag
      121 catcattgcc ggagctgttg gcgcgtttgc cggcatctgg atagccgtca aaacccaaga
      181 catgttttaa tcagatataa ttatttgtac aagggttctt agctcagctg gctagagcgc
      241 gtgattcaca ttcacgaggt cacaggttca agtcctgtag gacccaccac gaattatgaa
      301 aaagctacca gacaaaccaa ctttagctga ttatcaaacc tatcttaaag aggtgaatac
      361 tgagcggggt tttaaagaaa ccgtccagga cgtttttgtc ttacttgtgg aggaagtggg
      421 cgaatttgct aaagccataa gaaaaagggg cgtcattaaa gtcgacaaaa attccaagga
      481 acacaacgtt gaagaagaag cggccgacgt gttttatttg cttataaatc tctgcaacca
      541 cttgaatatt gacttagaga aagccttccg caacaaggag aagaaaaacc aaaaaaggca
      601 ctggtcgtga aaaaacatag cgcgggattg gtggtttaca ggaaaaaagc cgggcagctg
      661 gaagtgctgc tggcgcatat gggcacaccg tggtgggcca agaaagacaa gggcgcctgg
      721 actatcccca aaggcgagta tcaagagaac gaggacgcgc tggcggccgc caagagagag
      781 tttaaagagg agcttagcca cgaggtgccg gacggcaaat tgctggagct gggctcgatt
      841 gaacagaaca acaataagac agtggtcgcc tgggcgctgg aggccgactt ggacgtcagc
      901 catatcagca gcaataccac taccatagag tggccgccgc gctccggccg gacccaggaa
      961 ttcccggaaa tcgaccgggc ggcttattgg agcttgccgg aagcggcggc caggatcgtg
     1021 cccggccaga ccggcctgct ggagcgtctg gccgaaaaac tggccgtacc gttcgatccg
     1081 gcggccgaac caaagcaggg cagtcttttc tagcaacatt accccttatc agatacaatt
     1141 agacaaatgg gaaaatttta cgttactacg gctattcctt atgtgaatga caagccgcac
     1201 cttggccacg ccatgctgca cctgtacgct gacgtgctgg cccgctacca ccgccaaatc
     1261 ggcgataagg tcctgtttgc cgccgggacc gatgagcacg gcggcaaaat cgccgaaaag
     1321 gccgccgaag ccaagctgac gccgcagcaa ttcgccgacc aggtcagccg gagcttcata
     1381 gctctgctta aagagctgaa catcagcaat gaccgcttta tccgcaccac tgacgccagc
     1441 catgaacatc gcgcccagct catctggaaa aatctggcca agtatattta caaaggcact
     1501 tacgaaggct ggtactgcac cggcgacgaa gagtttttca gcgaacagac agtcaaagcc
     1561 aacaacggcg tctgtccgga ccacaaccgg ccatatgaaa aactgcgcga ggagaattat
     1621 ctgttcgccc tgagcaagtt cacgacccaa ataaaagagg ccatcgagac cgacgccttt
     1681 aaagtcatac cggatagccg caaacatgaa atcctgtcac tgcttaagag cggcctggaa
     1741 gatatcagcg tcagccggcc caaagacaag atttcctggg gtgttccggt gccaggcgat
     1801 aaagaccagg tgatgtacgt ctggtttgag gcgctgatga actacataac ggttttaggc
     1861 taccccgacc agccggattt taagctgttc tggccggcgg atgtacaggt aatcggcaag
     1921 aatattctgc gcttccacgc tgccatttgg ccggccatgc tcatcgggct gggcctgccg
     1981 cttcccaagc agctttatgc ccacggcttt attgaggtag acggcaaaaa aatgagtaaa
     2041 tcgctgggca acgtggtgga tccgctggac gttatcgcca aatacggcac cgacactttc
     2101 cgctactttt tcctgcgcca tatatcaagt tacgaagacg gcgatttcag ctgggcgcgc
     2161 ctggaagcgg cttataacga cgaattagcc gaccagctgg gcaacgccgt cgggcgcgtc
     2221 gccgcgatga ttaagaatta ccagaagggc ctgatcggcg acatcccggc gccggaccac
     2281 gacatcgcgc cctttcagca ggccctggcc gattgccgct ttgaccgggc ggtagaagag
     2341 atctgggacc aggtcaaagg cctcaaccag tatattgacg agaccaaacc ctgggagatt
     2401 gccaaagctg gtgacgccga ccacttgcgc gaggttctgg cctacgccgc cgccagcctg
     2461 atagagattg ccacgctgct gacgccattc ctgccggaaa cgtccgctaa tattctggca
     2521 gtgtttaaca ccggtgtgct gaagcctttg cccggaccgt tatttcccaa acacgaaaca
     2581 aaaaccggct gaacgactgc catggaattg atagataccc attgccactt gcattttgat
     2641 gattatgccg ccgaccgcca ggcagtgttt caggcctcgg cggctgccgg ggtcaagcgc
     2701 ttgatttgcg tcggcgtcag tctggccgac agccggcggg cgattgacct ggcccaaaaa
     2761 cagcccggcg tctgggccac ggccggcgcc cacccgcacg acggcgccga ttttttagcc
     2821 gacgggcaag caccagctaa gcttgcggca atgcttaaag agcccaaagt agtggccgtg
     2881 ggtgagatcg gtctggatta ttaccgcgac atcacgctta aagccgacca ggaaaaagtc
     2941 ttgcgaaccc agttggatgt gggcgcgccc agcggcttgc cctttgtctt ccacgtgcgc
     3001 gaagcttggg atggtttttg gccggtattt gactcgtata ggggaataac aggggttatt
     3061 catagcttca gtgccggtcc tgagcagctg gagcaggcct tgtcgcgcgg tctgcatata
     3121 gcgcttaatg gactaatgac ttataccaag gaagccagct ggctggcggc cgccaagcgg
     3181 gtgccgctgg acaggctggt gctggaaacc gacgcgccgt ttttgacgcc gaaaccctac
     3241 cgcggccagc gctgcgaacc caaacatacc gctgatacct gccgctttct ggcggagtta
     3301 aggaatgagg acccggcagt gctggccgcg gccaccaccg ccaacgccgt gaaacttttc
     3361 cgcctgggcg aagagcgtat cggatgaaaa gtatctatct ggattacgcg gctgccacac
     3421 ctgtggatga aaaagttata gcagccatgc aaccgttttt tagcgaccag ttctacaatc
     3481 cgtcggcgac ttatctggct gggcgggccg cgcgccgggc gctggatgaa gcccggtcgt
     3541 ccgtggccgg ctggttgggc gcgcggcccg gcgaaattac ctttacggcc ggcgccaccg
     3601 aagccaataa tctggtgatt tccggcctga tgcggcattt tccggatggc gaggtgctgg
     3661 tcagcgccat cgagcacgag tcggtactgg cgccggccag tcaatttcaa tgccgtcaaa
     3721 tcccagtcac cagacagggt ataaccgatt tggccaagct ggagaaaatg attacgccca
     3781 agacggtact ggtcagcgtg atgctggtca ataacgagct gggcactgtc cagccggtca
     3841 aagaagttgc caaaatactt aacaagtcaa gtattttacg caagggcgga ttacctctgt
     3901 atttacatag tgacgctgct caggcagcca gcctgtttga cctgcacgtt tcgcgcctgg
     3961 gtgttgacat gctaagcttt aacggcggta aaatttacgg ccccaaacaa agcggtgcgc
     4021 tgtacgtgcg ggccggtatt gagctgcagc cgctgatttt gggcggcggc caggagcgta
     4081 acctgcgcag cggcactgag aacctggcgg cggccgccgg cctggccaag gctttggagc
     4141 tcgccc
//
LOCUS       DASPTF010000034         4856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1343823, whole genome shotgun sequence.
ACCESSION   DASPTF010000034 DASPTF010000000
VERSION     DASPTF010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343823"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VEH48_01090"
     CDS             complement(<1..419)
                     /locus_tag="VEH48_01090"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXY18000.1"
                     /translation="MKIAVSGLGRMGGQIAQKLAEGGHEVIANNRSPEPIDQAVKHGA
                     KAAYKKEDVVAAFGGQPVVLWIMLPAEIIDAQLDEWLKLLPKGSLIIDGGNSDFRHDQ
                     ARAKRVQAAGSSFMDVGTSGGVWGYKNGFCMMAGGAEA"
     gene            complement(432..1097)
                     /locus_tag="VEH48_01095"
     CDS             complement(432..1097)
                     /locus_tag="VEH48_01095"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HXY18001.1"
                     /translation="MKFIKDASPQEAASYLADAINSELTGGQSVLWLVSGGSSVPVAV
                     ETIKLIPKTRWAGLCVGLVDERFGPPGHKDSNWQQLLTAGLPAVDLNILPILSGKDID
                     QTTAEYAAALQDRLAKANYKVGLFGIGADGHTAGILPLSVAIDSQQLVTDYITPEFQR
                     ITVTAKTIALLDLVVAYAVGAEKSEALQNLSRDLSVREQPAQALKLAKQLVIFNDQVG
                     DKL"
     gene            complement(1117..2442)
                     /locus_tag="VEH48_01100"
     CDS             complement(1117..2442)
                     /locus_tag="VEH48_01100"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXY18002.1"
                     /translation="MDGRPTILVVFGISGDLSHRYLLPALAVIKKAGQTPPDFKVLGL
                     SRKKLTAKDVYSGSTEPLATITEFLQMDYDDDADYLKLKKRIQAVSKDEHTQVIFYFA
                     VPPDATPAIIGHLGAARLNGPHAKLLLEKPFGNDLASARKLVRQTAKCFSEDQVYRID
                     HYLAKEVSQNVSTFLGSNVLFRKIWDKEFIEYIEVIVAEAIGLEGRANFYEKTGALRD
                     MVQSHSLQLAALTLMEPCPNPFDFSDLPQRRLAALKALSLAKLPTSVLRAQHEGYRQE
                     AGNPGSDVETFVALKLFSSSRRWRGVPVYLATGKKMDQKLTQIRVNFKKTNIAEANML
                     IIRIQPREGIELDLWVKKPGYEKKLEKKTLSFSFEQNFDRLPNAYEQVLVDAIRSDHS
                     LFTSSQEVLASWRILQPVLDYWQKTKGDLEFYRPGSSITDIVGGREVWN"
     gene            2497..3033
                     /locus_tag="VEH48_01105"
     CDS             2497..3033
                     /locus_tag="VEH48_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18003.1"
                     /translation="MARRLKSKYAIPGVILLVIVGLIVLVVRSLSAPAVGSVSQTPPS
                     QSQPTVPYNQPGTYKGQYVSFSYPAHYRPTPTGLSGSYLETVVYSSTDQSQKKIDVGI
                     SKGSPSTDGSVIYRQQHPELYRENTSAIWLEFIKLDGTEDTFFLQHGNLEASVSVSSP
                     YGDLGSDALYVASSLRWR"
     gene            3065..4126
                     /locus_tag="VEH48_01110"
     CDS             3065..4126
                     /locus_tag="VEH48_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18004.1"
                     /translation="MLRVIAGVLLGLGSLGGVFASLPTTTNYSLQSYGFGSGGGTSST
                     SNYSLEGITGELSGQSASTTNYSALPGFIQTQQANVPKVTLSNPSNYYDKLYFDIDQQ
                     NNPTDALYAMEISTTSDFSSGNNYVQMDHTIGPTLLLADYQTYSAWTTPSHNIIGLSG
                     STTYYLRALATQGEYTQSGWGPSSSAATVAQSVSFCLYTSGSCAGGGNTIAFGNIPVS
                     TITTSPNNIGTDFATNADYGGSVYIYSLNGGLKSASSGYTITSATADLSAVNEGFGAQ
                     VTSVSQTSGGPLSSQSPFNNSGNNVGILSAAVQILLNSNNPLVGGTAATQLQVKPSSI
                     AAAKSDYSETLTVIAAASF"
     gene            4203..>4856
                     /locus_tag="VEH48_01115"
     CDS             4203..>4856
                     /locus_tag="VEH48_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18005.1"
                     /translation="MRYLNLRLTKARIAGVIFAVALALQAVVPLLTPTASADQLTNTL
                     VRFDRMAQSQATTGTVCAKTTATVATEAVVSVFFPTGFTVSSTVGNWTVSTATTTGWP
                     TGANAWVGIGAPTGSGNFTISGQTVNFQSGDLAINTLYCFNWTSTAALTTGTAGAYTG
                     SVTTETSAPATISTGNYGVTTVGANADQITVTATVNPTFSMSLSANSDALGTLSTSSV
                     "
BASE COUNT         1072 a         1375 c         1260 g         1149 t
ORIGIN      
        1 gcctccgccc cgccggccat catgcagaag ccgtttttgt agccccaaac accgccgctg
       61 gtgccaacat ccatgaaact actgccggcg gcttggactc ttttggcgcg ggcttggtcg
      121 tgacggaagt ccgaattacc gccatctata atcagactgc ctttgggcag cagcttcagc
      181 cattcgtcca gctgggcgtc aatgatttcc gccggcagca taatccataa gaccacgggc
      241 tgaccgccaa aggccgctac cacgtcttct tttttatagg cggccttggc gccgtgcttg
      301 actgcctggt caatcggctc cgggctgcgg ttgttggcga ttacctcgtg gccgccctcg
      361 gccaattttt gggctatctg gccacccatc cgtcccaagc cgctaactgc tattttcatc
      421 tttgccctcc tttacaattt atcgcccact tggtcgttaa aaatcaccag ttgtttggcc
      481 agctttaagg cctgggccgg ctgctcgcgc accgaaaggt cacggctaag attttgaagc
      541 gcttcgctct tttcggcgcc gacagcgtaa gctaccacca agtcaagcaa agcgatggtc
      601 ttggcggtga cggtgatgcg ctggaactcg ggtgtgatgt aatcggtgac cagctgctgg
      661 ctgtctatgg cgacgcttag cggcaggatg cctgcggtgt ggccgtcggc gccgataccg
      721 aacagcccga ctttgtagtt ggctttggcc agccggtcct gcagcgcggc ggcgtattcg
      781 gccgtcgtct ggtctatgtc tttgcccgac aaaatcggca agatgttgag gtctaccgcc
      841 ggcaagccgg cagtcaacag ctgctgccag ttggaatctt tgtggccagg cgggccaaag
      901 cgctcatcta ccaaaccgac gcatagccca gcccatctgg ttttggggat aagtttgatg
      961 gtttcaacgg ccaccggaac ggatgagccg ccggagacca gccacaaaac gctctggcca
     1021 ccggtcaatt cgctgttgat ggcgtcggcc aggtagctgg cggcttcttg gggtgaggcg
     1081 tccttgataa atttcatggc atcaacgatt catagtttag ttccatactt cccgtccgcc
     1141 aactatatca gtaattgagc ttcccggccg gtaaaactcc aaatcgccct ttgtcttttg
     1201 ccaatagtcc aataccggct gcaaaatccg ccagctggcc aacacttcct ggctgctggt
     1261 aaataaactg tggtcggagc gaatggcgtc gaccagaact tgctcgtagg cgttgggcag
     1321 acggtcaaag ttctgttcaa aagaaaaact cagggtcttt ttttccagct ttttttcgta
     1381 gccgggcttt ttgacccaga ggtccagctc tatgccttca cgcggctgga tgcggataat
     1441 cagcatattg gcttcagcga tattggtctt tttgaagttg acccggatct gagtcagttt
     1501 ctgatccatc tttttgccgg tagccagata aaccggcact ccccgccagc gacggctgct
     1561 ggaaaataat ttgagcgcca cgaaggtttc aacatcagat cccgggttgc cggcttcttg
     1621 tctgtagcct tcgtgctggg cgcgcaggac cgaagttggc agtttggcga gtgacagtgc
     1681 ttttaaggcc gccaggcgcc gctggggcaa atcgctgaaa tcaaaagggt tggggcatgg
     1741 ttccataaga gtcagggccg ccagctgcag cgagtggctc tggaccatat cgcgcagggc
     1801 gccggttttt tcgtaaaaat tggcccggcc ttccaggcct atggcctcgg ccacaataac
     1861 ctcaatatat tcaatgaact ccttatccca gatttttcta aacagcacgt tgctgcccag
     1921 gaacgtactg acattttgcg atacttcttt ggccaggtag tggtctatcc tgtaaacctg
     1981 gtcctcgctg aaacacttgg cagtttggcg gacaagtttg cgggctgatg ccaaatcgtt
     2041 gccgaaaggc ttttccagta gcaatttagc gtgggggcca ttcagcctag ccgcacccaa
     2101 gtggccgata atcgccggtg tggcgtccgg cggcacggca aagtaaaaaa tgacttgcgt
     2161 atgctcatct ttactgactg cctgaatccg ctttttgagc ttcaggtagt cggcgtcgtc
     2221 atcgtagtcc atctgtaaaa attcggtgat ggtcgccagt ggttcggtgc tgccgctata
     2281 aacatctttg gcggtgagct ttttgcggct gaggcccaag accttgaaat ccggcggtgt
     2341 ctgcccggcc ttttttatca ccgccagcgc cggcaataga tagcggtggc ttaagtcgcc
     2401 gctgatacca aacaccacca ggattgttgg ccgcccgtcc atatatatta agtatatagc
     2461 tggcagtggc tttggtcgta gcgtaatatt taggtgatgg ctcgcaggct aaaaagtaaa
     2521 tacgctattc ctggggtgat actcctggta attgtcggtt tgattgtgct ggtcgtcaga
     2581 agtttaagcg cgccggccgt tggcagcgtc agccagacac caccgtcgca aagtcaaccg
     2641 accgtgccgt ataaccagcc ggggacctac aaggggcaat atgtctcatt ttcctacccg
     2701 gcgcactaca gacccacgcc caccggatta agcggcagtt atcttgaaac cgtcgtttac
     2761 agcagtaccg accaatcgca aaaaaagatt gacgtcggca tttctaaagg cagtccgagc
     2821 accgacggct ccgttattta ccgccagcaa catccggagc tataccgcga aaacaccagc
     2881 gccatatggt tggaattcat caaactggac ggcacagaag acacattctt cttacaacac
     2941 ggcaacctgg aggccagcgt atcagtcagt tcaccttacg gtgatctggg cagcgatgcg
     3001 ctatacgtgg ccagcagcct gcgctggcga taggtgttat aatttaggcc gatggggaaa
     3061 gctaatgctt agggttatag ccggtgtcct gttgggctta ggctctttgg gcggggtgtt
     3121 tgccagcctg cccacgacta ccaattacag cttgcaaagc tacggtttcg gcagcggcgg
     3181 cggcaccagc tccacctcta actactcgct ggaaggcatc actggcgaac ttagcggcca
     3241 aagcgcgtca acgaccaatt actcggcact gccgggcttt atacagaccc agcaagccaa
     3301 tgtgcccaag gtgactctgt ccaaccccag caattattac gataagctct actttgatat
     3361 tgaccagcag aataacccga ccgacgcgct gtatgccatg gagatcagca ccaccagcga
     3421 tttttcaagc ggcaacaatt acgtgcagat ggaccacacc attggcccga cattgctgct
     3481 agccgactat cagacctaca gcgcctggac aactcccagc cacaacatca tcggtctgag
     3541 tggaagcacc acttactacc tacgggcttt ggctacccag ggagaatata cccaaagcgg
     3601 ttggggaccg tccagcagtg cggccaccgt ggcccaatcc gtcagtttct gcctctatac
     3661 cagcggcagc tgtgccggcg gcggcaatac gatcgcgttc ggtaacattc cggtaagcac
     3721 aattactacg tcgccaaata atatcgggac cgattttgcc accaatgccg attacggcgg
     3781 cagtgtttac atatattcat taaacggcgg gctcaagagc gcctccagcg gctataccat
     3841 tacctctgcc actgcggact tatcggcggt aaatgaaggt ttcggggctc aggtcaccag
     3901 cgtaagccaa accagcggcg gaccgttaag cagccagtcg ccttttaata attccgggaa
     3961 taatgtcggc attctctccg ctgctgtcca aatcctgtta aattccaata atccactggt
     4021 cggcggtacc gcggccaccc agctccaggt caagccgtca tccatcgccg ccgccaaatc
     4081 cgactattca gaaactttaa cagttattgc cgccgccagt ttctgagatg gaagggttga
     4141 caaaacccta atgcttgctc acaatattgt ctaagggccg acaaagaaaa aaatatattc
     4201 taatgagata tcttaacctg cgacttacaa aggctagaat agccggtgtt atatttgcag
     4261 tagctttggc gctgcaggct gtcgtgccat tattaacacc tacggcttcc gcggatcagc
     4321 tcactaatac cttggtgcgc tttgaccgca tggcccagtc gcaagccacc actggtactg
     4381 tttgcgctaa aacaaccgct acagttgcta ccgaagccgt ggtctcggtg ttttttccca
     4441 ccggttttac cgttagcagc actgtcggga actggactgt cagtactgcc acgactaccg
     4501 gttggcccac tggggctaac gcctgggtag gtataggcgc ccccaccggc agcggtaact
     4561 ttactatctc cggtcaaact gtgaacttcc aaagcggcga ccttgctatt aataccttgt
     4621 actgtttcaa ctggaccagt accgccgcgc taaccaccgg gacggccggt gcttacaccg
     4681 gatcggtcac caccgaaacc agcgcgccgg caacaatcag tacgggtaat tacggcgtta
     4741 ccaccgtcgg cgctaacgct gaccagatta ctgtcacggc cactgttaat ccgactttca
     4801 gtatgtccct aagcgctaac tccgatgccc tgggtacgct aagtacatct tccgtg
//
LOCUS       DASPTF010000035         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_50336, whole genome shotgun sequence.
ACCESSION   DASPTF010000035 DASPTF010000000
VERSION     DASPTF010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50336"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VEH48_01120"
     CDS             complement(<1..260)
                     /locus_tag="VEH48_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18006.1"
                     /translation="MAHELPNPEETNAALTEEVWSAVEDFYKFVTEPERLAALGAALY
                     RQATSPDDIGIESILADIDPEQSRRALELEDVLKKLHGCVVT"
     gene            complement(303..1283)
                     /locus_tag="VEH48_01125"
     CDS             complement(303..1283)
                     /locus_tag="VEH48_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18007.1"
                     /translation="MSSFKRFFGIVILLALIAVPVVVWRQWNAIYDWSRLRDYSPPTD
                     VVSLADQDTFNAYTKHLFYVNHPQLLSTVTSFRQYCPENENTIVLGCYHPDQDGIYIY
                     NVQDPSLAGVAQVTAAHEVLHSIYARLSTKDRAALDSELQDYYQNDLHDQRVLAEVAL
                     YQQTEPNSVMDEMSCTFGTEIASLPSPLEAYYSRYFNNRQAIVAFEQNYEGEFTSRTN
                     QINAYDAQLAALKQQISNEESTLSAQLNQINSDRARLDSQRNSGDIGGYNAGVDSFNS
                     EVDSYNSGLNQLQANISDYNNLVTTRNSIAGELETLDQAIDTRLTTQSAQ"
     gene            complement(1313..>2140)
                     /locus_tag="VEH48_01130"
     CDS             complement(1313..>2140)
                     /locus_tag="VEH48_01130"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXY18008.1"
                     /translation="PTGVGKTELARVLAREFFGSEEALVKIDMSEFAEHHTVARLVGA
                     PAGYVGYDDGGQLTDKIRRQPYSVVLFDEIEKAHPEVFNTLLQVLEDGVLSDAKGRKI
                     DFTNTIVIMTSNIGAEKLQKEASFGFAAKKPSDLDNLDELHESNKSKVLDELKKMMRP
                     ELLNRIDKIIVFRALTKKDALQILDLQLEDLRARLVKKGIGLQVSADAKHHLLEKGYD
                     ALNGARPMRRLLQETLEDAIAGGLLDESYHKGDVVKVKTKKTAKTEEPELTYVSTPE"
BASE COUNT          417 a          567 c          597 g          559 t
ORIGIN      
        1 aatgtcacta cacagccatg cagttttttc agcacgtcct ctaattccaa agccctacgc
       61 gattgctccg gatcaatatc tgcaagtatg ctttcaatcc caatgtcatc tgggctcgtt
      121 gcctgccggt agagcgctgc cccaagagcg gctaatctct ctggctccgt aacaaactta
      181 tagaaatctt ctactgcgct ccagacttct tccgtcagtg ctgcgtttgt ttcctcggga
      241 ttaggtaatt cgtgcgccat tgcgatattt aagctaaaac tttctctgag agcaagcgct
      301 ggctactggg cggattgggt agttaggcgg gtgtctatgg cttgatccag cgtttccagt
      361 tcaccggcga tggagttcct ggtggtgact aggttgttgt agtctgaaat attggcctgc
      421 agctggttaa ggccgctgtt gtagctatct acttcactgt tgaagctgtc cacgccggca
      481 ttgtagccgc ctatatcacc agagttacgc tgggagtcca gacgagcccg gtcactgttg
      541 atttggttga gttgcgccga caaagtcgat tcttcgttgc tgatttgctg cttgagcgcc
      601 gccagctgag cgtcataggc gtttatctgg ttagtgcggc tggtaaattc gccttcgtaa
      661 ttttgctcaa aggccacgat ggcctggcgg ttgttgaagt aacggctgta gtaggcctcc
      721 agcggcgacg gcaggctggc gatttccgtg ccgaaagtgc agctcatctc gtccataaca
      781 ctattgggct cggtctgctg gtagagcgcc acctcggcca gcacccgctg gtcgtgtaaa
      841 tcgttttgat aataatcctg tagctcgctg tccagagcgg cccggtcttt ggtgctgagt
      901 ctggcgtaaa ttgagtgcag gacttcgtgg gcggccgtga cctgcgccac gccagccagc
      961 gagggatcct gaacattata gatgtaaatg ccgtcctggt cggggtgata acagcccaac
     1021 acgatggtat tttcgttttc cgggcaatat tgacggaagc tggtcacggt acttaaaagc
     1081 tgcggatggt tgacgtaaaa aaggtgtttg gtgtaagcgt tgaaagtatc ctggtcggcc
     1141 aggctgacaa cgtccgtcgg cgggctgtaa tcacgcagac gtgaccagtc atagatagcg
     1201 ttccactgcc gccaaaccac gaccggcacg gcgataagcg ccaacagtat tacaatccca
     1261 aaaaatcgct taaaggagct catctgtcct tcaagtataa caagccggta gcttactcag
     1321 gagtagaaac gtaggtcagt tcgggttctt cggttttggc ggtttttttg gtcttgacct
     1381 ttacgacgtc gcccttgtgg taactttcgt ccagcaggcc gccggcgatg gcgtcctcca
     1441 gcgtctcttg cagcaagcgg cgcatcggcc gagcaccgtt aagcgcatcg tagcccttct
     1501 ctaacagatg gtgcttggcg tcggcgctga cctgcagacc aatacccttc ttgaccagcc
     1561 gcgcccgcaa atcttccagc tgcaggtcca gtatctgcag ggcgtccttt ttagtcagcg
     1621 cccggaagac gatgatctta tcaatgcggt tcagcagttc cgggcgcatc atctttttga
     1681 gctcgtccag cactttgctt ttgttggatt cgtgcagttc gtccaggttg tccaggtcgg
     1741 acggtttttt ggcggcgaag ccaaagctgg cttctttttg cagcttttcg gcgccgatgt
     1801 tgctggtcat gatgacaatg gtgttggtga agtcaatctt gcggcccttg gcatccgaca
     1861 gcacgccgtc ttccagcacc tgcagtaagg tgttgaaaac ttcggggtgg gctttttcga
     1921 tctcgtcgaa aagtaccacg ctgtaaggct ggcgacggat tttgtcggtc agctggccgc
     1981 cgtcgtcata gccgacatag cctgccggcg cgccgaccag acgggcgaca gtgtggtgct
     2041 cggcgaattc gctcatatct atcttaacca gcgcctcttc cgaaccgaag aattcgcggg
     2101 ccagcacccg ggccagttcg gtcttgccaa ctccggtcgg
//
LOCUS       DASPTF010000036         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1487239, whole genome shotgun sequence.
ACCESSION   DASPTF010000036 DASPTF010000000
VERSION     DASPTF010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487239"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            454..539
                     /locus_tag="VEH48_01135"
     tRNA            454..539
                     /locus_tag="VEH48_01135"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:488..490,aa:Leu,seq:taa)
     gene            complement(541..789)
                     /locus_tag="VEH48_01140"
     CDS             complement(541..789)
                     /locus_tag="VEH48_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18009.1"
                     /translation="MMYTAIGIIGAVLVLFGFYRTSIGKWTGKSRLYELDNLVGASLI
                     TIYQWHYRVYVTVVVNIIWAIVALHGLTSYKQRRKAKK"
     gene            complement(789..>2308)
                     /locus_tag="VEH48_01145"
     CDS             complement(789..>2308)
                     /locus_tag="VEH48_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXY18010.1"
                     /translation="IVVYPRNESDVRKVARFSWQLAERKRVLPITARGGGSDTSGAAI
                     GSGIMLVFTAHMNRILSLDPRKEFVTLEPGVTYDKLEQTLYTHGLFLPPCPASKQFAT
                     IGGGLANNALGEKSVKYGATAQYVEQLRVVLANGEVIETGPLSKRQLNRKLGLSSLEG
                     QIYRGIDKLLEENADFIKREQEQIKARWNAIGYNIFDVKKKGNFNLTPLILGSQGSLG
                     IITEATLEVVGYNPRTTLLLASLNDLDSLNDVLPKILALKPSSLEMINQAAISQVTAI
                     NPHQLSGLMQNPGAAIHLFIEFDNLKEGAQKAGAKKARKITEQAGGVTQLANTAPEQE
                     KLWKIRHSVATILSEPQGHAKAIPITEDICVPVQSLADFLQKAEAIYRQNDLVASAWG
                     HAGDGIVRMQPVLDLAQLGDRQKLFRLTDDIANLVISLGGTLSASAGDGRVRAPYAGK
                     QYSDGLLKLMLEVKKIFDPHGILNPGVKTASMEDVRTLLRGDYNLNHRHEHLPRS"
BASE COUNT          496 a          587 c          638 g          587 t
ORIGIN      
        1 catctgttct tctatctcgg tgaagttctt gaccgcccag ctgaacagtg cgaaactgag
       61 cgccagaccg actataaaaa tggcgagcgc gatgggcgag ttccattgcc aaaccggttg
      121 cattagaccc tcctagccca ccgcttttta atgattgtac ctaaacaata gcgcttattt
      181 gcagcgaacg caacttatag atgtggatat cttgtataat tgtggttgtt ctttgtctta
      241 tccaaatttg caaagcggag gtctaatcac cgtttgcaaa tttgagagga gcaacatagc
      301 gctaagccga agcttttgct aaatcggcaa aacgaggcag gcgcgtaagt tgcgacgagg
      361 cgcgatggcc gaggagttag gcaagggtct gcaaaaccct gtacacgagt gcgaatctcg
      421 ttcgcgcctc cagaacatat ttgacaaaat atcgggaaga tggcggaatt ggtatacgcg
      481 tttgacttaa aatcaaattc ccaaaaggat tgagggttcg agtccctctc ttcccaccaa
      541 ttattttttg gctttgcggc gttgtttgta tgatgttaag ccgtgcaggg cgactatcgc
      601 ccaaatgata ttcaccacaa ctgttacgta gactctataa tgccattggt aaatggtaat
      661 caggctggcg ccgaccaggt tgtccagctc ataaagccgt gatttgccgg tccacttgcc
      721 gatgctggtg cggtagaaac cgaacagcac cagtaccgcc ccaatgatac cgatagccgt
      781 atacatcatt aacttctagg caggtgctcg tgccggtggt ttaggttgta gtcgccacgc
      841 agtagcgtgc gtacatcttc catggacgcc gttttgacgc cggggttgag gatgccgtgc
      901 ggatcaaaaa tcttttttac ctccagcatc agtttgagca ggccgtcgct gtattgtttg
      961 ccggcatacg gcgcgcgaac ccggccgtcg ccggccgatg cgctcagtgt cccgcccaaa
     1021 gagatcacca ggttggctat atcgtcggtc aggcggaaaa gtttttgcct atcgcccaac
     1081 tgcgccaaat ccaaaaccgg ctgcatgcgg actatgccgt cgccggcgtg tccccaggcg
     1141 ctggctacca ggtcgttctg gcggtaaatc gcttcagctt tttgtagaaa atccgccaga
     1201 ctttgtaccg gcacgcagat atcttcggtt atcggtatgg ccttggcgtg cccctggggc
     1261 tcgcttaaga tggttgccac gctgtgccgg attttccaga gcttttcctg ctcaggagct
     1321 gtgttagcca gctgggtaac gccgccggcc tgctccgtga tcttgcgggc ttttttggcg
     1381 ccggcttttt gcgcgccctc ttttaagttg tcaaactcaa tgaacagatg gatagccgca
     1441 ccgggatttt gcatcaggcc ggacaattgg tgcgggttga tagctgttac ctggctgatg
     1501 gccgcctggt tgatcatctc caggctgctg ggtttgagag ccagaatttt gggcaaaacg
     1561 tcatttagac tgtcaaggtc attcaggctg gccagcagca atgtggtgcg cgggttgtag
     1621 ccaacgactt ccagagtggc ctcggtgatg atgcccagac tgccctgaga gcccaaaatc
     1681 aggggtgtca gattaaaatt ccccttcttt ttgacgtcaa aaatgttgta gcctatggcg
     1741 ttccagcggg cttttatctg ttcctgctcg cgtttgatga aatcggcatt ttcttccagc
     1801 aatttatcta tgccgcggta gatttggcct tctaaagaag agaggcccag cttgcggttg
     1861 agctgacgct tgcttaaagg tccggtctct atgacctcgc cattggctaa aaccacccgt
     1921 aattgttcca cgtactgtgc cgtagcgccg tatttgacgg acttttcgcc gagcgcgttg
     1981 ttggccaggc cgccgccgat ggtggcgaat tgtttggaag cggggcaggg cggtaaaaac
     2041 aggccatggg tgtaaagtgt ttgctccaat ttgtcataag ttacgcctgg ctcgagagtg
     2101 acgaattcct tgcgcggatc caaagagagg atgcggttca tgtgagcggt aaagaccagc
     2161 atgataccgc tgccgatagc cgcgccggaa gtgtcactgc cgccgccgcg ggcggtgatg
     2221 ggcagaacac gtttacgctc ggccagctgc cagctgaagc gcgccacttt gcgcacgtcg
     2281 ctttcgttac gcgggtaaac gacaatag
//
LOCUS       DASPTF010000037         9301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1487521, whole genome shotgun sequence.
ACCESSION   DASPTF010000037 DASPTF010000000
VERSION     DASPTF010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487521"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VEH48_01150"
     CDS             complement(<1..540)
                     /locus_tag="VEH48_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18011.1"
                     /translation="MAENGQRLLLDVLRADQFTSDHIELLNNRADQFQKNPERLRAKE
                     ALSRLAVTQDLERLLPEELAPVLSEEVIELTASRLLEQVYTKLGSLAAYFWFDEPSTR
                     TVGSFDAARALLGMERLGHTNAKLASSTAKGETLHDSMRTANEHLKAFGGGVVIIRHP
                     EPGSAAEAAEIMDFPVINAG"
     gene            complement(594..1220)
                     /locus_tag="VEH48_01155"
     CDS             complement(594..1220)
                     /locus_tag="VEH48_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXY18012.1"
                     /translation="MTPTEPQPKPDYSQFDPRKQTPQRGDNRRNYAFIDSQNLNLGIQ
                     KVGWKMDWRKFRQFLKKEYGVEKAYMFIGYIADNISMYEQLHESGYLIVLKRTLEVKN
                     PDADKKPESAEPAKPTVKGNIDSELVLWAMKELPHYDKAIIVSGDGDFFALVEYLEQQ
                     HKLLHLLVPNWQYSSLLKDYEKYIVRLDKLRRQLSYRDRRHSHKPKQA"
     gene            complement(1268..1738)
                     /locus_tag="VEH48_01160"
     CDS             complement(1268..1738)
                     /locus_tag="VEH48_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18013.1"
                     /translation="MIGKWLHRLALAVLALLVVAGVAGLAAWRVSGLKLYVMSDNHLA
                     PGLQRGDLLLVAPNKTPKPGDFVNYVNPGNPRDVITEKLSGRVPDAGYVGVTVKAVPL
                     AGYALRQLRTPLGLGLLVYLPAAVIVGGEIKCLRPTASAYQFIAFTPVAKPATL"
     gene            complement(1745..2323)
                     /locus_tag="VEH48_01165"
     CDS             complement(1745..2323)
                     /locus_tag="VEH48_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18014.1"
                     /translation="MRHRRSKLAVIRSLLVILTVGVATTGISFAALQGTPVSLTGNTI
                     NTGTVDLRISKDGITFTTPSIAGFSFDGVVPGGAAVPAAGNTVYLKNNGNVNIALKAG
                     ISTTPLNNSGVDLTKTHLTFTRTDSGGPPVTVSVKALIDGYAGGGTSLGDTIAAGTTA
                     IYSLKASMDADAYSGASASISNITLVFSGTGA"
     gene            complement(2441..3061)
                     /locus_tag="VEH48_01170"
     CDS             complement(2441..3061)
                     /locus_tag="VEH48_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXY18015.1"
                     /translation="MRVTRYFQSCLLIEEGGVRILIDPSGQDAGNFDKFGKLDAVLYT
                     HEHMDHFDPELDEKFARAGVRRYANASTAKQMKTPPTVVKDGQELEINGLKVMAKELP
                     HCLMVTGETSAQNTGYLVAGRLFHSGDGTQISGLSAEAVAVPLTGPDISLKDAYDLIR
                     SLAAKIAVPVHYDFIGTKPEVFQRFSQRAQPTFELKILRRGETADL"
     gene            complement(3075..3452)
                     /locus_tag="VEH48_01175"
     CDS             complement(3075..3452)
                     /locus_tag="VEH48_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXY18016.1"
                     /translation="MLGDKTAVATIAVKDMEVAKKFYERTLGLTKSGDDPGGILYKSG
                     DSNVFVYQSEFAGSNKATAASWGVGGDMDKIMAGLKAKGVKFEQYDIPGVKREGDVHV
                     IGELKAAWFKDPDGNILNIVNQM"
     gene            complement(3524..3757)
                     /locus_tag="VEH48_01180"
     CDS             complement(3524..3757)
                     /locus_tag="VEH48_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18017.1"
                     /translation="MTSTGEDLQAEPLLETDRQYYYFPGIIMGFVLAPEVVQRVREAA
                     EAAEDNREKNQKPPKPPKRPNARIGYVALAGNT"
     gene            complement(3779..4150)
                     /locus_tag="VEH48_01185"
     CDS             complement(3779..4150)
                     /locus_tag="VEH48_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18018.1"
                     /translation="MITQEYRLGGSEHNRPTEDPDRVQACIEIAKEIAERRKDKNHNL
                     LDRVLEEIARGNPDLFGFTSRAIGNGKVIEERTKQIKLERAQDASSGYFIIPPEPEQL
                     ETTNPPSGDAEQEQPDAITNS"
     gene            complement(4198..4656)
                     /locus_tag="VEH48_01190"
     CDS             complement(4198..4656)
                     /locus_tag="VEH48_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXY18019.1"
                     /translation="MEIPELIKNYLQEVKVMQLATTRNNQPWACSVHFVFDDKFNLYW
                     MSTKERRHSREIIDNNLVAATIPIKYPVNPLVGISVEGGAEIVDRLQAQDLIKLYDDR
                     FGLSDKFKSDYFMGTQAHELFKLTPRLYVLYDEINFLDNPRQEWRPAGNP"
     gene            complement(4701..5066)
                     /locus_tag="VEH48_01195"
     CDS             complement(4701..5066)
                     /locus_tag="VEH48_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18020.1"
                     /translation="MERVLDIEDVEVEGILERIRDMGATVMSFSSRVAAVPLETDFQE
                     MMKIHGLGFQDRFREWPNVSPRIIELFGGNSLSLAERQGLATGVLDVALRHGRLRGNG
                     ILFIDHSDDPEGIILGDRF"
     gene            complement(5198..5980)
                     /locus_tag="VEH48_01200"
     CDS             complement(5198..5980)
                     /locus_tag="VEH48_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18021.1"
                     /translation="MSEIEQQSEILSVSEAYKKYPDQRNNVDLLAAVYHATRPRRFEM
                     SRAMKIYLQIWIALAGISMVRAYESRQIQGDVRPEVKAQFERVLKPEAISLGKIVVAE
                     AQDKRTMVKSIKKDKDVEGGVVVTVDEWDGLTDYQIQARMLQKAGNPEPGTTYEADAM
                     LFNCDNPKCKDPELTEVDIYQAEIDKVQGKSQIPDGHIWSADFSYFPLHSRSASRTFE
                     AGFSKLDSSLASAEVQVALSNAEFINKIAQVIGKSSVGAGNT"
     gene            complement(6044..6688)
                     /locus_tag="VEH48_01205"
     CDS             complement(6044..6688)
                     /locus_tag="VEH48_01205"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HXY18022.1"
                     /translation="MKLLLTSGGISNHSIAKAFGELVSKNPSEVKVGYIPIAANVEAG
                     NKDWVIKDFLNLWRYGYSQIDIVDPSAAGVNWETRLADVDVVALSGGNTFHLLDQVRK
                     TGLDGWLKTNLGNKVYVGGSASSILVTPSIGAAGIGSFHDENLPNLKDLAGLGFVDFE
                     IVPHVPGWGTYKDCEQYSAKTSNKLYALDDSSAIKIDGDKVEIISEGVWKLYNG"
     gene            complement(7330..7923)
                     /locus_tag="VEH48_01210"
     CDS             complement(7330..7923)
                     /locus_tag="VEH48_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18023.1"
                     /translation="MKTVFIIVSSLLTVVCTLPYILDVIKRKTKPREVTWLNWSLLAA
                     LAASASLSDHQYAAAALSFCGTIETLAVFVLGWRYGDKSIARFDIACQTAAIIGLALW
                     FIFNSPAIAVLASITIDFIATLPTIRHSWQKPTEETWITFFLAGIAAGFTVAAAKEAR
                     ITSLANPIYLVAINLLLAGIIIYRHPSIKFRYGAGNA"
     gene            complement(8047..8823)
                     /locus_tag="VEH48_01215"
     CDS             complement(8047..8823)
                     /locus_tag="VEH48_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18024.1"
                     /translation="MYKSVEAKEYKKHFEMPEDYRIDGVLISGTWQVDREFERVEQLL
                     KQSHPSAKIQPIPYRFVSIGREITTDKGRYWVFVMYGGAMLSEFLHLGSLFGSKKNIL
                     VGLAGGLKPGIKMADIILPSASFKDGSMSTMYDPSDNELVHADKGLTSSLKTRLDPKL
                     AIHEGKTMTCQAMMGESAEDVKKWSTAGYLAVEMEAATVFAVSKHFKVPAAAVLSIAD
                     NLIEEETVMHEKYEALAEIRSNVRNEQYRIALDELLAGNT"
     gene            complement(8838..>9301)
                     /locus_tag="VEH48_01220"
     CDS             complement(8838..>9301)
                     /locus_tag="VEH48_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXY18025.1"
                     /translation="MDGVIPPEKRFKLILSVYLILVQDGKTLLLRRQNTGYEDGNYGL
                     VAGHADGNEVAREALCREVLEESGLKFKPEDFRFVHLMHRKANDERIDVFFTADSWQG
                     EPEIMEPEKCDDLSWFSLDNLPKNTIPYIRQAIECSQKDIYYSEHGWTGNT"
BASE COUNT         2084 a         2395 c         2189 g         2633 t
ORIGIN      
        1 gccggcgtta attacgggga aatccataat ctctgccgct tccgcggcgc taccgggctc
       61 agggtgtcta attataacta cgccgccacc aaatgcctta agatgctcgt tagctgtacg
      121 catactgtcg tggagagttt cgcctttggc tgttgaggac gctagtttag cgttagtgtg
      181 tccgagccgc tccattccca gtaatgcccg cgctgcatca aagctaccca cagttctggt
      241 agacggttcg tcaaaccaaa agtaggcagc cagactccct agctttgtgt agacttgttc
      301 aagcagtctg ctagcggtta attctatgac ttcttcggat aatacaggcg ccagctcctc
      361 gggaagtaat cgctcgaggt cttgtgttac agctagtctg ctcagtgctt ctttggctct
      421 aagccgttca ggatttttct ggaactggtc agcgcgatta ttaagcaatt cgatatgatc
      481 ggacgtaaat tggtcagcgc gcagcacatc caatagaagt ctctgcccat tctcagccat
      541 actgcaacca tattcagcta tgtcacgcta aagtgcaagc ttaagcggtt tgctcaagct
      601 tgtttgggct tgtgggaatg ccgacggtcg cggtaggaca gctggcggcg cagtttgtcc
      661 aggcggacga tgtatttttc gtaatctttt agcaggctgg aatactgcca gtttggcacc
      721 agcaagtgca gcagtttgtg ctgttgttcc agatattcca ccagagcgaa aaagtcgcca
      781 tcgccggaaa ctatgatggc cttatcgtag tgcggcagtt ctttcatggc ccagagtacc
      841 agctcagagt caatgttccc cttcactgtc ggcttggccg gctcggcact ttccggtttc
      901 ttatcggcgt cggggttttt gacctccagc gtgcgcttca gcactattaa gtagccggat
      961 tcgtgcagct gctcgtacat cgagatgttg tcggctatat agccgatgaa catataggcc
     1021 ttttccacgc cgtactcttt tttgaggaat tggcggaatt tgcgccaatc catcttccag
     1081 ccgactttct ggatgcccag gttgaggttc tggctgtcta taaaggcgta gttgcgccgg
     1141 ttgtcgcctc tttggggcgt ctgttttcgg gggtcaaatt gtgagtaatc aggcttgggt
     1201 tgtggttcgg tgggtgtcat aaattcattc ttaacagagg ttaattatac tattttttaa
     1261 cgctatatta aagtgtcgct ggctttgcaa caggggtaaa agcgatgaat tggtaagcgc
     1321 tggcggttgg acgcagacac tttatttcgc ctcccacaat cactgcggcc ggtagatata
     1381 ccagcaggcc gaggcccagc ggtgtgcgca gctggcgcaa agcgtagccg gccagcggca
     1441 cggccttaac agttacgccc acgtaaccag catccgggac tctacccgat agcttttcag
     1501 taatcacgtc gcgtgggtta cccgggttaa cgtagttcac aaaatcgccc ggtttaggcg
     1561 ttttgttcgg cgccaccagc aataggtcgc cgcgctgcag ccctggcgca aggtggttat
     1621 cagacatgac gtataacttc aggccggaaa cccgccaggc cgccaagccc gctactccgg
     1681 cgaccaccag caaagccagg accgccagcg ccagccggtg caaccacttg ccgatcatcg
     1741 ctacctaagc tccggtgccg gaaaacacca gcgtaatgtt gctgatgctg gcgctggcac
     1801 cgctgtaggc gtcagcgtcc atcgaggcct ttaaactata gatagccgtg gtgccggcgg
     1861 cgatagtgtc gcccaaactg gtgccgccgc cggcatagcc gtctatcaga gcttttacgg
     1921 atacggtgac cggcggtccg ccggagtcgg tgcgagtaaa agtcaggtga gttttggtta
     1981 ggtcaactcc gctattatta agcggcgtgg tgctaattcc cgctttgaga gcaatgttga
     2041 cgttgccgtt gtttttaaga taaaccgtgt tgccggcggc cggcacggcg gctccgcccg
     2101 gaacaacgcc gtcaaaacta aaaccagcta ttgaaggcgt agtaaatgta atgccgtctt
     2161 tactaatgcg caggtcaacg gtgccggtgt tgatggtatt tccagtcaac gacaccggag
     2221 tgccctgcag agccgcgaaa ctgatgccag tggtcgctac gcccaccgtc aagatgacca
     2281 gcagtgaacg gataacggct aatttggacc ggcggtggcg catagtagct gagtttctcc
     2341 tgataattta ctaaatatgc caccgccgcc agcgccggtc aatatttttt tgcaagttac
     2401 ttttacaacg cgcggttttt atgttgaagc actagctttt tcagaggtcg gcggtctcac
     2461 cccgcctgag tattttgagt tcaaaggtcg gttgggcccg ctgcgaaaac ctttgaaaaa
     2521 cctccggctt ggtaccgata aagtcgtagt ggaccggcac ggcgattttc gcggccaagc
     2581 ttctaatcaa gtcataggcg tcttttagcg atatgtccgg gccggtcagc ggcacggcga
     2641 ccgcttcggc gctcaggccg cttatttgcg tgccgtcgcc gctatggaaa agccggccag
     2701 cgaccaaata gccggtattt tgagcgctgg tttcgccggt gaccatcaga caatgcggca
     2761 gttctttagc cataactttt aggccgttta tttctagctc ctggccatct ttaactacgg
     2821 ttggcggcgt cttcatctgt ttggcggtgc tggcgttggc atagcgccgt acgcccgccc
     2881 tggcgaactt ttcatccagc tccgggtcaa agtggtccat atgttcatgc gtgtatagta
     2941 cggcgtccag tttgccaaac ttgtcaaaat tgccggcatc ctggccgctg gggtcaatca
     3001 gaatccgtac cccgccctct tcaatcaaca aacaactctg aaaatatctt gttactctca
     3061 tgcgatactc ctagttacat ctggttgacg atgttcagta tgttgccgtc cggatccttg
     3121 aaccacgccg ccttgagctc gccgatgacg tgcacgtcgc cttccctttt aactccggga
     3181 atgtcgtact gttcaaactt cacgcccttg gccttaagac cggccatgat tttatccata
     3241 tccccgccca cgccccaact ggcagccgtg gctttattgc tgccggcaaa ctccgattgg
     3301 taaacaaaaa cgtttgagtc cccgcttttg tacaaaatgc cgcccgggtc atcgcccgac
     3361 tttgtcagcc ctaaagttct ttcgtaaaat tttttggcca cttccatatc cttgaccgca
     3421 atggtcgcga ccgctgtttt gtctcccagc atcccaaacc tcctttttat atccttatta
     3481 taccctagct gcgaatcttg attttagcaa aatacttaac ttgttaagta tttcctgcta
     3541 gcgctacata gccgattctg gcgttggggc gttttggcgg ttttggcggc ttttgatttt
     3601 tttcgcggtt atcttcagcc gcttccgctg cctctcgcac tctttgaacc acttccggcg
     3661 ctagtacaaa acccattata atgccgggga aataatagta ttgcctgtca gtctcaagca
     3721 gaggttcagc ctgtaaatct tcgccagtac tggtcatttg ctattgatct gatttgtttt
     3781 aggaattcgt gatcgcgtct ggttgttctt gctcggcatc tcctgatggt gggttagtag
     3841 tttctagctg ttccggctct ggggggataa taaaataacc agaggatgca tcttgcgccc
     3901 gctccagttt aatttgtttt gtccgttcct caatgacctt gccgttgccg atagcacggc
     3961 tggtaaagcc gaacaaatcg ggattacccc tggcaatttc ctcaagtacg cgatccagta
     4021 agttatggtt tttatcctta cgcctttcgg ctatctcttt cgctatttca atgcacgctt
     4081 gcactctatc cggatcttcc gtcggtctgt tatgctcaga cccgcccaaa cgatactctt
     4141 gtgtgatcat attttatatt ttacaactga tatcatcttt tgtcaatctc gtggggatca
     4201 gggatttccg gctgggcgcc attcctggcg gggattatcc aagaagttga tttcatcgta
     4261 aagtacgtac aatcttgggg tcagcttaaa taactcgtgg gcctgcgtgc ccataaagta
     4321 gtccgatttg aatttatctg ataaaccaaa ccggtcatca tacagtttta tcagatcttg
     4381 cgcctggaga cggtcaacaa tttctgcccc gccttcaacg gaaatcccca ccaaaggatt
     4441 aaccggatat tttataggga tggtcgctgc caccaggtta ttatcaatta tctcccggct
     4501 gtggcgccgc tctttagtgc tcatccagta taggttgaat ttgtcgtcaa aaacaaagtg
     4561 aacgctgcaa gcccatggct gattattcct ggttgtagcc agctgcatga ccttcacctc
     4621 ttgcaagtaa ttcttaatca gctcaggaat ttccataaat taattctaca ctggttactg
     4681 aattatattt tactccggtt ttagaatctg tcgcccagaa ttatgccttc cggatcgtcg
     4741 ctgtggtcaa taaaaagtat gccgtttccg cggagacggc cgtgccgaag cgcaacatcc
     4801 aggacaccgg tcgccaatcc ctgtctttcg gctaagctca ggctgttgcc gccgaataat
     4861 tctataatcc tgggcgatac attaggccat tcgcggaaac ggtcctgaaa accaagacca
     4921 tgaattttca tcatttcttg aaagtcggtc tccaggggca ccgcggctac tctgctggaa
     4981 aaactcatga cggttgcgcc catatctcgg atcctttcca ggatgccttc aacttcaacg
     5041 tcctcaatat ccaggaccct ttccattgca gccaggacaa tattaccctc tccgtatctt
     5101 atgcgcgcgt tcattaacct gcccccggta tctaaatatt atactctcaa caatatcccc
     5161 tgttgtttga gtcgcctgcc ttgaatttat cgaagggtta agtatttcct gccccgacgc
     5221 tagacttacc tataacctga gctatcttgt ttataaactc ggcattcgac aaagcgactt
     5281 gaacctcggc tgaagctaaa gagctatcta gcttactgaa acccgcttcg aaagttcggc
     5341 tggcgcttct tgaatgcagt gggaagtagc taaagtcagc ggaccagatg tgcccatctg
     5401 gtatctgtga tttaccttga actttatcga tttcagcttg gtatatatca acctcagtta
     5461 gttcggggtc tttgcatttg gggttatcgc agttaaatag catagcgtcc gcttcgtaag
     5521 ttgtgcctgg ctcaggattg cccgcctttt gtagcattct agcctgaatc tgatagtcgg
     5581 tcagaccatc ccattcgtca acggtaacta caactccacc ctctacatct ttgtccttct
     5641 tgattgattt aaccattgtt cttttatcct gtgcctctgc aacaacgatt ttgcctaagg
     5701 atatggcctc aggttttagg acccgttcaa attgggcttt tacttccggc cggacatcac
     5761 cttgaatctg cctagattca taggcgcgca ccatgctaat tccagcaagg gcgatccata
     5821 tctgaaggta tattttcatt gcccggctca tctcaaacct tcttggcctt gttgcgtgat
     5881 agacagctgc cagcaaatcg acgttgttcc gctgatccgg gtatttttta taggcttccg
     5941 atacggatag gatttcagac tgttgttcaa tttcagacat ggttttattt tacgctggta
     6001 tcgagaattt ggtaaaatac ttgacttgtt aagtattacc tgctcagccg ttgtagagtt
     6061 tccagacgcc ttcgctgata atctccacct tgtcgccgtc aatctttatg gccgatgaat
     6121 cgtccagcgc gtaaagttta ttggaggttt tagccgagta ctgctcacag tctttgtatg
     6181 tgccccagcc cggaacatgc gggacgattt caaaatctac gaaccccaaa cctgctaagt
     6241 ctttaaggtt aggcagattc tcgtcatgga acgaaccgat gcctgcggcg ccaatgctgg
     6301 gtgtaactaa aatgctgctg gcgctgccgc ccacgtaaac cttatttccc agattagttt
     6361 tcagccatcc atccagtccg gttttccgca cttggtctaa cagatgaaag gtgttgccgc
     6421 cgcttaacgc caccacatca acatctgcca atctggtttc ccagttcacg ccagcggccg
     6481 atggatcaac aatgtcaatt tggctgtagc cgtaacgcca caagttcaag aaatctttaa
     6541 taacccagtc cttgttcccc gcctcaacat tggcggctat cgggatgtag ccaactttga
     6601 cttcgctggg atttttactg accagttcgc cgaaggcttt ggcgatagag tgatttgata
     6661 tcccccctga ggtcagtaag agtttcatgg ttttatttta cgctggtttt gagaaatccg
     6721 aagtgcttag ctataaatag atttttttgc gccagggttt cccctggcgc gggggcggga
     6781 gcgttgactc ccgccccgga ctttattgag ttgcggctga acctagcgac taagaagcaa
     6841 atcaagcatg gcgtcgacct cccaaatctg gtctaggctc tcgggcaaaa atggcccctt
     6901 gcttcttagc cggttgccgg acatcaggac gccagtgtct agcaaacccg tctcgagcaa
     6961 tacaatctgc cgcgagtact gtccgacgct caccgtccct aagtaccagc cagtaagctc
     7021 caatacatca tctcggggcc ggaagaagaa ttgccaccaa cccctccaga ctggaaccgg
     7081 cgactgaacc atgactacgt ttgttcctgc cttacgcctg aacaagtccg cggcgccagt
     7141 ttccgccttc tttccgagat tttgtagctg ttgtagcacc actgcttcgt gagaggccga
     7201 aggcccagca gaactcaaag tccacctcca gattagattg tttcgcgact acctatagcg
     7261 gccgctagtc agatctttcg gattataaaa ttatacaatt tttaattaga ttggcaatta
     7321 cttgacttgt taagcattcc ctgctccgta tctgaatttt attgaaggat ggcgatatat
     7381 gataattcca gccagcaata aattaatcgc tactaggtat attggattag caagagatgt
     7441 gattctcgct tccttagccg cagctaccgt aaaacctgct gctatgcccg ccaggaaaaa
     7501 agttatccaa gtttcttcag ttggcttttg ccatgagtgt cttatagttg gaagagtcgc
     7561 tatgaaatca atcgttatgc tggctaaaac ggcgatggct ggcgaattaa aaataaacca
     7621 gagggcaaga ccaataatcg ctgctgtctg acaggcaata tcgaaccgtg caatgctttt
     7681 atcgccatac ctccagccaa gtacaaatac agctaatgtc tcaatagttc cacagaaaga
     7741 tagggcagct gctgcatatt ggtggtcgct taaagatgcc gatgcggcaa gagcggcgag
     7801 caaagaccaa ttcaaccacg ttacttcccg cggcttagtt tttcgtttaa taacatccaa
     7861 tatgtatggc aatgtgcaga caaccgtcag caaactgctg acgattataa aaactgtttt
     7921 catatgggtt ttattctacc ccgtagtagt taataacact atggggcggg ctctaaaatg
     7981 cggggtggat gagggcggcg ggttttattt tacgctggtt ttgggaaatc caggttgttt
     8041 gggttgttaa gtattccctg ccaaaagttc atcgagcgcg attctgtatt gttcattacg
     8101 gacattgctg cgaatttcgg ctagggcttc gtatttttcg tgcatgaccg tttcttcctc
     8161 aataaggttg tctgctattg acaacaccgc tgcagcggga actttgaagt gttttgatac
     8221 ggcaaacacg gtcgccgctt ccatttctac agctaaataa cccgcagttg accacttctt
     8281 aacgtcctca gcggactcgc ccatcatggc ctggcaggtc atggttttgc cttcatgaat
     8341 tgccagttta ggatcaagcc gggtttttag gctgctggtc aagcctttat ccgcatgcac
     8401 tagctcgtta tccgagggat cgtacatagt tgacatacta ccatctttaa atgaggccga
     8461 aggcaagata atgtctgcca tttttatacc tggttttaaa ccccctgcta ggccgactaa
     8521 aatattcttt ttgctgccaa acagcgaccc gaggtgcaaa aattcgctga gcattgcccc
     8581 accatacatc acaaacaccc aatagcgtcc cttgtctgtc gttatctccc tgccgatact
     8641 gacaaagcga tacggaatcg gctggatttt tgccgaagga tgactttgtt tgagcagttg
     8701 ttctactcgt tcgaattccc tgtctacttg ccatgtgcca gaaattaata cgccgtcaat
     8761 tcggtagtct tcgggcatct caaagtgttt tttatattct tttgcttcta ctgatttata
     8821 cataattgct tgacttgtta agtattccct gtccagccgt gttcggaata gtaaatgtct
     8881 ttctgtgagc attcaattgc ttggcggatg tatggaatgg tgtttttggg taagttgtcc
     8941 agcgaaaacc agcttaggtc atcgcacttt tctggttcca tgatttctgg ctcgccttgc
     9001 cagctgtcag cggtaaagaa aacgtctatc cgctcatcgt tggctttgcg gtgcatcaaa
     9061 tggacaaacc gaaaatcttc cggcttaaac ttcaggccgg attcttccag cacttccctg
     9121 cacagcgcct ctctggccac ttcgttgcca tcggcgtgcc cggctaccag gccataatta
     9181 ccgtcctcgt aacccgtgtt ttggcgtctg agcaatagag ttttaccgtc ttgtaccaat
     9241 attaaataaa ccgagagaat cagtttaaat cttttttccg gtgggataac cccgtccatc
     9301 t
//
LOCUS       DASPTF010000038         8738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_927533, whole genome shotgun sequence.
ACCESSION   DASPTF010000038 DASPTF010000000
VERSION     DASPTF010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8738)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927533"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VEH48_01225"
     CDS             <1..480
                     /locus_tag="VEH48_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18026.1"
                     /translation="NVPNQYQKVKKTPFLLGLVTIVLGLAIIFLSTARAAGLRRIGWP
                     LLVVGIVLLAFAWAINRVTVHNLAPKISVNNAVLQSSLRTLIVDVEQAVDKSYWWFGG
                     GYAALGLGAIGGAMYIKKHNGSKKPAAATEDSAPAEKPPAAPAKPAAPKPRRTIKVQ"
     gene            complement(580..1314)
                     /locus_tag="VEH48_01230"
     CDS             complement(580..1314)
                     /locus_tag="VEH48_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18027.1"
                     /translation="MSEQETGNPEKPSNKDFVQKNIEFLESRGFAGQLMIPTFKAALE
                     VEPRLKAVLFDVYRTVPNQHVLAGASAPWEDPERGLYTILWNPEFFGANEEVLTKALP
                     IELKGDPYIEKQCEMIGKDFHDTALSDIDQSILLHELGHIVYFLDNEDNFKEIEASRQ
                     RDLEGLPVVPGALIAELPDFKERYETNKDGVRDQLNEDYGVSSLEELYKVQMNAYHNM
                     PSEMIPDEFAAKVFEHIREKRNEEQG"
     gene            complement(1370..1446)
                     /locus_tag="VEH48_01235"
     tRNA            complement(1370..1446)
                     /locus_tag="VEH48_01235"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1410..1412),aa:Arg,seq:gcg)
     gene            complement(1490..2026)
                     /locus_tag="VEH48_01240"
     CDS             complement(1490..2026)
                     /locus_tag="VEH48_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18028.1"
                     /translation="MKPEQETNLYRPDDTAATDSGQKSRKTKAISWTASEYIEHQHGA
                     GWYLSLVVLTAALAAILYLLTKDYFAAGVIVVLGIIVGVSAGRKPRQMSFELSSSGLK
                     AGEKSYSYNLFKSFSVLHEGAQTSLNFIPVKRFMPPVSAYFEPKDEDKITSLVGEHLP
                     YEERRPSAIDRLSFRLRL"
     gene            complement(2107..2916)
                     /locus_tag="VEH48_01245"
     CDS             complement(2107..2916)
                     /locus_tag="VEH48_01245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXY18029.1"
                     /translation="MTAQLTNLSIQIINYNSKKYLESCLDSLSKDLETANFSYTISVL
                     DNNSGEDLRDLQARHKSKQVKFYESDKNLGFAAGHNWLAGRQKSQSILMLNPDVEFIQ
                     PQTIMRLLHRLQAQNNSKVVGPRLINAEGIQQLWDHGEISGHKAYVADKVNRSVWRER
                     DKPAKVAWVSGAVFLIERAIFDQLGGFDENFFMYGEEEELCYRLRKSGRDVIYDPTIT
                     ALHHGGVATSKNDRYAYHKRSQAYFIQKHAAKRFSYPLWRNLDKKINNRGE"
     gene            complement(2935..3027)
                     /locus_tag="VEH48_01250"
     tRNA            complement(2935..3027)
                     /locus_tag="VEH48_01250"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2991..2993),aa:Ser,seq:gct)
     gene            3243..4238
                     /locus_tag="VEH48_01255"
     CDS             3243..4238
                     /locus_tag="VEH48_01255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXY18030.1"
                     /translation="MRKTISIVLPVHNELGNLALIYKKLIKTLRSLKYDLELVFVNDG
                     SQDGSMAKLVKLADDDPRVMVIDLSRNFGHQLAITAGIDYAKGDAIITMDSDLQDPPE
                     ICPLLIKKWEEGYDVVYAKRRNRQGDSLFKRLSAQLFYGLLNKMSETSIPSNVGDFRL
                     IDKKVAVELRKLREHQRFLRGLVSYVGFKQTIVLFDRARRHSGKTGYPLKKMLGFARD
                     GIISFSTVPLRIITRLGYLVSLLSLAGIIYAVSIKLFDPARAVPGWAFVTVAIFFIGG
                     VQFIMLGLLGSYIARIYTEAQDRPLYIVAAIHGKRAARSPQSDSSLSLVAKTQRS"
     gene            complement(4190..4666)
                     /locus_tag="VEH48_01260"
     CDS             complement(4190..4666)
                     /locus_tag="VEH48_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HXY18031.1"
                     /translation="MPLPTKQLRFGLVGVFNTLFNLLLFNIFIFFLGISVAYANVLSL
                     LISILISYFLNAKFVFAPHPSRNSYTTFGLFMSVSLFSQLVVQQLVVVVLSQHVTGPG
                     RLMAAALRHLPLLVHLSERFYVVNTAKCLAIALSLLVNFLLYDRWVFATKLKEESD"
     gene            4833..6173
                     /locus_tag="VEH48_01265"
     CDS             4833..6173
                     /locus_tag="VEH48_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18032.1"
                     /translation="MHPRHLLVLKVAVVFGLLLGISWYRLDPDFGWHLAAGNYIRAHG
                     IPAHDIFTYTARNFPWIDHEWGNDVIVSWLYQLGGYFVLCILYAALWASALFVNGRRS
                     LKVLSLAAVAFLPYAGIRPIAWTVLGFALLLRFTSDKSRFPKLAIPLIFIIWANLHGG
                     FVIGLVYLAYLAIARRSRSWGLILLLSIFATFINVYGPRLYVEIARTLFDRSLHSQIS
                     EWEPFYILGPSWPYIILWMSGFWLFSRKKISHWLRFDTLLLVAGFSSSRNIPLFIVAS
                     LGNTDAYLKKALGMIPKRLDWPRKLVLGGLAIAVVISVGYGLKATYWPFAPRESAYPV
                     QAVAYLQTNACPGNLFNDYNYGGYLIWKLPSEPVYIDGRMPSWRAPDGIKYLDTYLQV
                     IKNKTVRQQQFTKYNIRCALLRNTVTELKMAKQLKSIGWRQVVQTNGSTLLIKR"
     gene            complement(6337..6423)
                     /locus_tag="VEH48_01270"
     tRNA            complement(6337..6423)
                     /locus_tag="VEH48_01270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6386..6388),aa:Ser,seq:tga)
     gene            complement(6455..7048)
                     /locus_tag="VEH48_01275"
     CDS             complement(6455..7048)
                     /locus_tag="VEH48_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXY18033.1"
                     /translation="MSAFGERCAYCSGLSPRGRVCIHCRQTGAPRFVWITANHETMAQ
                     HLVRVYKFGHSRSAVHPLADLMANTFLSYVSPEQARSADFLIVPLPTATSRIRQRGFD
                     HAALLAKALSKRLGLPYAQVLGRLGQSRQVGSSRAARLAQQSGNYFVRQPQRVAGRNL
                     LIIDDVITTGGSLLAASRALRAVGATHVDALVFAKRL"
     gene            7572..8051
                     /locus_tag="VEH48_01280"
     CDS             7572..8051
                     /locus_tag="VEH48_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXY18034.1"
                     /translation="MARGSQQQQNDDLLMGDDITAAFLSEEEVAAEPAAAGGAADDAA
                     NWDEEEVVAGQLAVDVYETKEKLVVKGRVAGVNKSELDVSIADNTLTIKGTLNAGEDE
                     GVENYFLQECYWGEFSRSLVLPVPVKEEEIEAVLKDGVLTISFSKLKQDTVKKIEVL"
     gene            complement(8117..8542)
                     /locus_tag="VEH48_01285"
     CDS             complement(8117..8542)
                     /locus_tag="VEH48_01285"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HXY18035.1"
                     /translation="MNNRVLVLRLLGPVLALVMSLGLVSSVAYAQNAQGQINNGLCTG
                     ANLQFTDNPSGASCGGTDATTSINNLVRTIVNLLSAIVGIVAVIMIIVGGLRYITSGG
                     NDTSVTGAKNTILYAIVGLIVVALAQLIVRFTLSKVTNS"
BASE COUNT         2180 a         2162 c         2216 g         2180 t
ORIGIN      
        1 aatgtgccca accaatatca aaaagttaag aaaacaccgt ttttgctggg tctggtgacc
       61 atcgtgctgg gactggcgat tatcttctta agtacggccc gggcggccgg gctacggcgg
      121 atcggctggc cgttactggt agtcgggatt gtacttctgg cctttgcctg ggccatcaac
      181 agggtcacag tccataatct ggcgcctaaa atcagtgtca ataacgccgt gctgcaaagc
      241 agcctgcgca ccctgatagt tgatgtggag caggcggttg ataaaagtta ctggtggttc
      301 ggcggcggct acgcggcgct gggcctggga gccatcggcg gagctatgta cataaagaag
      361 cataacggta gtaaaaagcc ggccgcggct accgaagatt cggcgccggc cgaaaaaccg
      421 ccggcagcgc cggccaaacc ggccgcaccc aaacccagac gaaccatcaa agtccagtag
      481 ttatactggt acaggatact ggaccaggtt agaaccattc tgattgttaa acctttaggc
      541 tcggattaaa agcactcgta cttcctatta gtctttttat tagccttgct cttcattcct
      601 cttttcccta atgtgctcaa atactttagc tgcaaattcg tccgggatca tttctgaagg
      661 catattatga taagcattca tttgaacctt ataaagttct tctaagctac ttacgccata
      721 atcttcgtta agctgatctc tgactccatc tttgttagtc tcgtaccgtt ccttgaaatc
      781 aggtagttct gctatgagtg ccccaggaac aactggtagg ccttctaagt ctctctgcct
      841 tgatgcttca atttctttaa agttatcctc attgtccaag aaataaacta tatgccccag
      901 ttcatgcaga agaatactct ggtcaatatc tgaaagtgct gtatcgtgaa aatctttacc
      961 tatcatttcg cactgctttt caatataagg atcacctttg agctctatag gtaaagcttt
     1021 agtaagcact tcttcatttg caccgaaaaa ttcgggattc cataaaatcg tataaagacc
     1081 tctttcggga tcttcccagg gtgctgatgc tccagctaaa acatgctgat taggaacagt
     1141 tctgtagacg tcaaacaata cagccttaag cctaggttct acttcaagag cagctttaaa
     1201 agtaggaatc ataagctgac ctgcaaatcc ccggctctca agaaattcta tatttttctg
     1261 aacaaagtct ttattggaag gcttttcggg attaccggtt tcttgttctg acatttctgt
     1321 ttgagattaa cccttctgta atagcataag ggatataata agtcaatgat ggtacgcgtg
     1381 gtaggatttg aacctgcgac cttcggctcc gcaagccgac gctctatcca gctgagctac
     1441 acgcgcgcaa caggggtaat tatagcattt acctctgtaa attaccagtc tagagtctta
     1501 ggcgaaaaga caggcggtcg atggcgctgg gacggcgctc ttcgtacggc aggtgctcgc
     1561 cgaccaggct ggtgatttta tcttcgtcct tgggctcaaa gtaggccgag accggcggca
     1621 taaaccgttt gaccggtatg aaatttaggc tggtctgggc gccttcgtgc aaaacgctga
     1681 aggatttgaa caaattgtag ctgtagctct tttcgccggc cttgaggccg gagctggaga
     1741 gttcaaaact catctgccgc ggtttgcgac cggcagacac gccgacaata attcccagaa
     1801 ccactatgac gccggcggcg aaatagtcct ttgtcaacag atataatatg gctgccaagg
     1861 ctgccgttaa gacgaccagc gatagatacc agccggcacc atgctggtgc tcaatatatt
     1921 ccgacgctgt ccagctgata gcttttgttt tgcgggattt ctggccgcta tcggttgcag
     1981 ctgtatcgtc gggcctataa agattggttt cttgctccgg cttcatgcta ctgcttattg
     2041 tagctaatcc ccaaaaataa aaatacttga cgattcgact acctcaggat attcagggtg
     2101 gtggctttat tcgcccctat tgttaatctt cttatccagg ttgcgccaaa gcggatagct
     2161 aaagcgcttg gcagcatgtt tttgtataaa gtaagcttgg gagcgcttgt gataagcgta
     2221 tctatcattt tttgaagttg cgactccacc gtgatgcaat gccgtaatgg tcgggtcata
     2281 tattacatcc cgccctgact ttcgtaaccg atagcagagc tcttcttcct cgccatacat
     2341 gaagaaattc tcgtcaaagc cgccaagctg atcaaaaatt gccctttcaa tcaaaaaaac
     2401 ggcgccggat acccaagcga ctttcgcggg cttatcccgt tcccgccaaa cactccgatt
     2461 cactttgtcg gccacatacg ccttatggcc acttatctcg ccgtggtccc agagctgctg
     2521 gatgccctca gcgtttatca atcttggccc gacgaccttt gagttatttt gggcctgcag
     2581 ccgatgaagc aagcgcatga ttgtttgcgg ttgaataaat tcgacatccg ggtttagcat
     2641 cagaattgac tgcgattttt gtcgaccggc taaccagttg tgtccggcgg caaaacctaa
     2701 atttttatcg gattcgtaaa atttgacctg cttactctta tgccttgctt gtagatccct
     2761 gaggtcctcc ccggaattat tgtccagcac actgatcgtg taagaaaaat tcgccgtctc
     2821 taaatctttg gatagtgaat caaggcagct ctcaagatat tttttgctgt tgtagttaat
     2881 gatctgaatc gacagattgg ttaattgtgc ggtcactgca gtttagattc ttcctggcgg
     2941 agagggaggg atttgaaccc tcgcggggac ttgcgccccc ctatcgattt agcaaaccga
     3001 ccccttcagc ctcttgggta cctctcctcg tcgcaacctg cgcgcttgcc tcgttttacc
     3061 gaatggggta aaagcttcgg cttagcgcta tgtcgctcct cccaatttca caaacggtcg
     3121 cttgccgccc cgcttggcaa aattgtagag aacagagaga attataacag attggagcgc
     3181 tgtttcgcat tctgcagaat gccgaaaata ttatatagta ggaacggcat caggaggttc
     3241 ttttgcgcaa aacaatcagc atagtcttac cggttcacaa cgagctgggc aaccttgccc
     3301 taatctacaa aaaacttatc aagactcttc gctcccttaa atatgacctc gagctggtct
     3361 tcgtcaacga tggcagccaa gacggctcaa tggccaagct ggtcaaactg gccgatgatg
     3421 atcctcgggt aatggtcata gacctatcca ggaatttcgg ccaccagctg gctataaccg
     3481 ctggcataga ttacgccaag ggcgatgcca tcataaccat ggacagcgac ttgcaggacc
     3541 cgccggaaat atgtcccctg ctgattaaga aatgggaaga aggctatgat gtggtctacg
     3601 ctaaaaggcg caaccggcag ggggacagct tatttaaacg gctatctgcc cagctctttt
     3661 acggtctact taacaagatg tccgaaacca gcatcccgtc aaacgtgggg gactttcggc
     3721 taattgacaa aaaggtggcg gttgagctgc gcaagcttcg tgagcaccag cgttttttac
     3781 gcggcttggt cagctatgtc ggatttaaac agacgatagt gctgtttgac agagcccgac
     3841 ggcacagcgg caaaaccggt tacccgctaa agaaaatgct gggctttgcc agagacggga
     3901 ttataagttt ctcaactgtg cccctaagga ttatcactcg cttgggctac ctggtatcgc
     3961 ttttaagcct ggccggaata atttatgcag tcagtattaa actttttgac ccggcacgag
     4021 cagttcctgg ctgggctttt gtgacagtag cgatattttt cattggcggc gtacaattta
     4081 ttatgctcgg gctcttgggc agttatatcg cccgcatcta tacggaagca caggaccgtc
     4141 ccctgtacat agtcgctgcg atccatggta agcgagcagc ccgcagtcct cagtctgact
     4201 cttctttaag tttagtagcg aaaacccagc ggtcataaag caaaaaattc accagcagcg
     4261 acaaagcaat ggccagacac ttggcggtgt tcaccacgta aaacctttct gagaggtgca
     4321 cgagtagagg caggtggcgc aaggcagcgg ccatcagccg gcccggcccg gtcacatgct
     4381 gactgagcac gacgactacc agctgctgga ccaccagctg gctgaacaga ctgactgaca
     4441 taaacagacc gaaagtagtg tagctgttgc ggctggggtg aggcgcaaag acaaatttag
     4501 cattcaggaa atagctgatc aggattgata tcaataatga taggacgttg gcataggcta
     4561 cagagattcc tagaaagaaa ataaatatat tgaagaggag caggttgaaa agcgtgttaa
     4621 acacgcctac taggccgaat ctcaattgtt tggtgggtaa cggcatcctc tccattcatt
     4681 ctagaccagg tccagtgtct tgggaccaat ttacgcatgg gttgaagtat aatgaccctt
     4741 aagtgaaggc caagaaacaa agcagggaaa ttactcgcgg ctggtttggc cgcttcaacg
     4801 cccttaaggc aaaggtttta ttatcgacta aaatgcatcc gcgccactta ctggtcctca
     4861 aagtggccgt ggtattcggg cttttactgg gtatctcgtg gtacaggtta gaccccgact
     4921 ttggctggca tctggcagcc ggcaattata tacgagccca cggtattcca gcccacgata
     4981 tctttaccta caccgctaga aatttccctt ggattgacca cgagtggggt aacgatgtca
     5041 ttgtttcttg gctgtaccag cttggcggct attttgtttt atgcattttg tatgcagctt
     5101 tgtgggcctc ggcgctattc gtcaatggcc gcaggagcct gaaggtgctg tctttggcgg
     5161 ccgtggcttt tctaccatat gccggcatta ggccgatcgc gtggacagtc ctcggttttg
     5221 ctttgctgtt aagattcacc tctgataaaa gccgtttccc caagcttgct atccctttga
     5281 tctttataat ctgggccaac ctgcatggcg gctttgtgat cggcctggta tatttagctt
     5341 atttggctat agcccggcgc tccagaagct ggggcctcat tttactgtta agtatctttg
     5401 caacatttat taatgtttac ggaccgcggc tgtacgtaga aatcgcccgg accttatttg
     5461 accgctccct gcattcacag atctccgaat gggaaccatt ttacatactg ggtccgtcgt
     5521 ggccctacat tatcctctgg atgtcaggtt tctggctctt ttccagaaaa aaaatcagcc
     5581 attggctgag attcgacacc ctgttgctag tcgccggatt ctccagtagc cgcaatatcc
     5641 ctctatttat agtcgcctca ctaggcaaca ccgacgctta tctcaaaaag gcgcttggca
     5701 tgattcccaa gcgcttggac tggccgcgta agttggtatt aggtgggtta gctatagcgg
     5761 tagttattag cgtgggttat ggactgaaag ccacttattg gccgttcgcg ccacgtgaaa
     5821 gcgcctatcc cgttcaggcc gtagcttact tgcaaaccaa tgcctgccct ggtaacttgt
     5881 tcaacgatta taactacggt ggatatttaa tttggaagct gccgtccgag ccggtctaca
     5941 ttgacggccg gatgcccagc tggcgggcgc cagatggcat caaatatcta gatacatacc
     6001 ttcaggttat aaaaaataaa actgtccggc agcagcagtt taccaaatac aacatccggt
     6061 gcgcgctctt aagaaacacg gttaccgagc taaaaatggc caaacagcta aaatccatcg
     6121 gctggcgaca agtcgtccaa accaacggct ccacgctatt gataaagcgc tagcctatgg
     6181 cctgagaggg cagaagatac ttaacaagtc aagtatttgc aactgatggg ggccccattg
     6241 catggctctg atgcaatggg gtgaagcgta agaacgtgcg gaagctgaaa gcaaattgct
     6301 taagctattt gcggaagctg aagtcacggc ctaagctggc ggaaggggtg ggattcgaac
     6361 ccacgaagct ttcgcttggc ggttttcaag accgctgcac tagaccaact atgcgaccct
     6421 tccttaccct gaatatttta ctcctcttta atggctagag gcgtttggcg aagacgaggg
     6481 catccacgtg ggtagcgccg acagcgcgca gagcgcggct ggcagccagc aggctgccgc
     6541 cggtagtaat gacgtcgtca atgattaaca gattacggcc ggccacccgc tgcggctggc
     6601 ggacaaagta gttgccggat tgctgcgcca ggcgcgccgc ccgcgaagaa ccgacctggc
     6661 ggctctggcc caagcggccg aggacttggg catacggtaa gcctaagcgt ttggataaag
     6721 ccttggccaa cagggcggcg tggtcaaatc cgcgctggcg gatacggcta gtggcggtag
     6781 gcaagggcac tatcaaaaaa tccgctgatc tggcttgctc cggtgagaca taacttaaga
     6841 atgtgtttgc catcaggtcg gccagaggat ggactgctga ccgggaatgg ccaaatttat
     6901 aaacccgcac cagatgttgg gccatggtct cgtgattggc ggttatccag acaaagcgcg
     6961 gcgcgccggt ctgccggcaa tgaatgcaga cgcgcccgcg ggggctgagg ccgctgcagt
     7021 aggcgcaacg ttctccgaaa gctgacatgc cagagccaga acaatcttgg cagagaaccc
     7081 ggccttcagc gccacagcca atgcactccg gcggcgcgat caacccaatc gctgtctcca
     7141 acaaactcat ccgtctatca tcaaaaatcg gccgacgata gtcaacctta agcgctttaa
     7201 tcgcacccta cacatttgtg cgcagtacaa aagcgagctg gcttaagcac tccgcgcttg
     7261 gatgcagcgc aaggaagaga tgagtaacgg actgagctgt attgcaatac agtgaaggag
     7321 taacgcaagc tctgacacag tgctgcgcca agagccgctg gaacagctgg ctgggccggc
     7381 tgtctagcgg cggcgcggag tgcgttgtaa ttgttctgtt gtaaatgttg ggtataacat
     7441 gttgcacgtc ttgccggcgc taagctatac taaaatcaac aaaaagatgc aaggtgcgcc
     7501 aaagcaaaat taactaaaac ctgcccgtgt tatagctgtt ctactttagc gggtacgagg
     7561 aggattcagg catggctcga ggcagccaac agcagcagaa tgacgattta ttgatgggcg
     7621 atgacattac cgccgctttt ttgagtgagg aagaagtagc cgcggaacca gcggcagccg
     7681 gcggagcggc agacgacgcc gcaaactggg atgaagaaga agtggtggcc gggcaactgg
     7741 ccgtagacgt ttacgagacc aaagaaaagt tagtcgtcaa gggccgcgta gccggcgtaa
     7801 acaagagcga attagacgtc agcatagccg ataacaccct gactatcaaa ggtacgctca
     7861 acgccggcga agatgaaggc gtggaaaact actttttaca agagtgttac tggggtgaat
     7921 ttagccgcag cctggtactt ccggtaccgg tcaaagaaga agagattgaa gctgtgctta
     7981 aagacggcgt actgaccatc agcttctcca agctcaagca ggacacagtc aagaagattg
     8041 aggtcttgta gttaagtctg gctttgacaa aacaaaaaag ccccggctcc ggggcttttt
     8101 tgttatcaga ctccgtctaa ctattagtca ctttgctaag cgtaaagcgg actattagct
     8161 gagccagggc gaccacaatc aggccgacga tggcgtataa aatggtgttc ttggctccgg
     8221 ttacactggt gtcgttgccg ccgctggtga tgtaacgcag tccaccgacg ataatcatga
     8281 taaccgccac tatgccgacg atagcggaaa gcaaattgac tatggtgcgg accaggttat
     8341 taatgctggt cgtggcatcg gtgccgccac agctggcgcc gctggggtta tctgtaaatt
     8401 gtaagttggc tcctgtgcag agtccgttgt taatctgccc ctgagcattc tgggcatagg
     8461 ccacggacga aaccaggcca agtgacatca ctaaagccaa cactggccca agcaatctca
     8521 gtaccaaaac tctattgttc atatcccaca ttatagatta agtcggcagt taatagctac
     8581 cccttggcca agaaggcgat tttatgtagt agctttattc agcacgaacc ggactatgat
     8641 ttgcgctaaa gcgacgatta tcagaccgat aatggcatag aggatggtat tcttggcccc
     8701 ttggacgctt tcctgcttgc caccgctggt gatgtagc
//
LOCUS       DASPTF010000039         3859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1208643, whole genome shotgun sequence.
ACCESSION   DASPTF010000039 DASPTF010000000
VERSION     DASPTF010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208643"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1943
                     /locus_tag="VEH48_01290"
     CDS             <1..1943
                     /locus_tag="VEH48_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18036.1"
                     /translation="INGLNIGLDQGANRTDTEYIDDVSVDTVSTGTASSLNVDDSLHV
                     GGTSNFGSNVNIQSAGTGSSPLTINDATTGINLLQVKDLNQNFGSAVTAGAFLQRTSY
                     FGEEFNKFLSTNTDSTTSTTTNTNSRRGDNTELSTSSLIGATAGSCAYSSNSNALGGT
                     ERIAAASSTGTQADCSELLSTATAGTSQKLWAANNLPIVQMKVQPSAAPTGNSNTRFF
                     YGLSDNNTSAAGVPTNGIFFTNCTTTTPTCTSAQWVGMVIVGGTVAGTVTCTAGAETG
                     TINTTGFNYLRIEVRSNTDIHFFVDTTTTDGIVETECGSGVQATGPGATALGLYMAGG
                     VNATGLTLNTDIDYVRVWQDDAPVDNSSDNSAASNNSTADTTDLSSAPPISPDSPDPS
                     IAGSFFNFLGATSEDTVINGNLFVHGTIYADKIEANQIEGLQIYTDELGSLQQQLNAV
                     KADTTTANGSGGSEDSSSSESSSGSDGALSLDISASGLTVGGDATFQGNVFFYKLVTF
                     VQKTVFNDDVTFGGHITTAGTTPTVNLETAAGITVAPPIDPNNLNAPAPNLAKDTISG
                     NDNSGQLSITFGDNASAGELLTVNFNKPFDNAPNIFLSPTNQAAAGLKYYITSTTTGF
                     KITVTDPLTAGTTMNFSYWVVQ"
     gene            1986..2480
                     /locus_tag="VEH48_01295"
     CDS             1986..2480
                     /locus_tag="VEH48_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18037.1"
                     /translation="MDRSNLRDYTRAPLREHPTSRTPAVAEQEPRPAARKRRKLPNVL
                     QWRYLAAVLLLAIVVYLAYGYIHTKHQLDKLSNPQVATQDETQQLVSEVGKLVQLPSG
                     ETPVLFTVNDASKLKSFSFFQDAQNGDKVLDYPKADKAVLYRPSANRVIVYTTVNLTN
                     APSQ"
     gene            2545..3309
                     /locus_tag="VEH48_01300"
     CDS             2545..3309
                     /locus_tag="VEH48_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXY18038.1"
                     /translation="MAKKIIAILNQKGGVGKTTTAINLAAYLAKAGHKVLLCDIDPQA
                     NSTSGLGLDKRALNSTLYDVLFSRAEAHKTIRKVTSHGIYLLPANPDLAGAEVELVEI
                     PYRELKLKSVLDNLNYDYILIDCPPSLGLLTVNALAAADEVLIPVQAEYYAMEGLTQL
                     LATIQQVRAALNPNLNILGVVVTLYDNRNSLAEQVRAELQNHFGDKIFVTVIPRNVRL
                     AEAPSFGKTIVEHDKWSKGARAYKSLAKEVEERTNG"
     gene            3302..>3859
                     /locus_tag="VEH48_01305"
     CDS             3302..>3859
                     /locus_tag="VEH48_01305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXY18039.1"
                     /translation="MAKKQALGRGFEALIPKDFDASMLEEDKHRVQKILIQDISPNPQ
                     QPRREMDEAALAELAESIKTHGVLQPIIVVKAGNGYQIVAGERRWRAAKAAKLSHMPA
                     IVRSLKELERIELSLIENIQRVDLSPLEQALSVFKLQQQFNLALEDIAKKLGKAPSTI
                     SNLTRLLQLPESARQALKDGRISEGH"
BASE COUNT         1052 a         1059 c          935 g          813 t
ORIGIN      
        1 acattaacgg cctgaacatt ggcttagacc agggcgccaa ccgtaccgat accgaataca
       61 tagatgacgt atcggttgac acggtcagta ctggcacggc aagctcgctt aatgttgatg
      121 acagcctaca tgtaggcggc acttccaact ttggcagcaa cgtaaacatc caaagcgccg
      181 gcacaggctc gtcaccgcta acgataaatg atgccaccac cggcataaat ctgttgcagg
      241 tcaaagatct caaccagaat tttggctcgg ctgtcacagc cggtgcattt ttgcaaagaa
      301 cttcatattt tggcgaagaa tttaataagt tcctgtctac taataccgat agcactacaa
      361 gtacaaccac caatacgaac tccaggaggg gcgataatac tgaactctca acaagctcac
      421 tgataggtgc gacagccggg tcctgtgcgt atagctctaa tagtaatgcc ttgggcggaa
      481 ccgagcgtat tgctgccgcc agttcaaccg gcacccaggc tgactgttcc gaactattaa
      541 gtacggctac agccggcacc agccagaaac tctgggcagc taataacctg ccaattgtac
      601 agatgaaagt tcaaccaagc gccgcgccga cgggcaactc caatacacgc ttcttctacg
      661 gacttagcga taacaatacc tcggcggctg gtgtcccaac caacggtatt ttctttacca
      721 actgcacaac cacaacccca acctgtacaa gtgcccagtg ggtcggcatg gttatagttg
      781 gaggcacggt tgcaggaacg gtaacttgta ccgctggtgc tgaaaccggc acaattaata
      841 ccaccggctt taactacctg cgcatagaag ttcgttctaa cactgatata catttcttcg
      901 ttgacacaac taccactgac ggaatcgttg agacagagtg cggttctggt gttcaagcaa
      961 caggaccagg ggctacggca cttggccttt acatggcggg cggtgttaat gccaccggat
     1021 taactctaaa cacggacata gactatgtca gggtctggca ggatgacgcg ccggttgaca
     1081 attcttcaga taactccgcg gcttcaaata attcaactgc cgacaccacg gaccttagct
     1141 ctgctccgcc aatttcacct gattcaccgg acccaagtat agcggggagc ttctttaact
     1201 tcttgggagc caccagtgaa gatacggtta tcaacggtaa cttgttcgtg cacggcacca
     1261 tttacgccga taagattgaa gccaaccaga tagaaggtct gcagatttac accgatgaat
     1321 tgggcagctt acagcagcag ctcaacgccg tcaaagccga taccacgact gccaacggct
     1381 ccggcggcag cgaggatagt agctccagtg aatcttccag cggcagtgac ggcgccctga
     1441 gcctggatat ttcggccagc ggcctgactg ttggcggcga tgccaccttc caaggcaacg
     1501 tcttcttcta taaactggtc acattcgtgc aaaaaactgt ctttaacgac gacgttacct
     1561 tcggcggtca tattactaca gccggcacta cgccaacggt caatctggaa actgccgccg
     1621 gcattactgt cgcaccgccg attgacccca acaatctaaa cgcaccagcg cccaacttgg
     1681 ccaaagacac aatttctggc aatgataact ccggccagct gagcataacc tttggcgata
     1741 atgcttctgc cggcgaactg ctgactgtca acttcaataa gccgttcgac aacgcgccca
     1801 atatcttctt gtcgccaacc aaccaagccg ccgccggcct caagtactac atcaccagta
     1861 ccaccactgg ctttaagata accgtaaccg acccgctgac tgccggcacc accatgaact
     1921 tcagttattg ggtcgtccag taggttgcca caagggttaa aactcgctaa acttaagcgg
     1981 taaacatgga taggtcaaat ctaagggact atacccgcgc tccactgcgg gaacacccca
     2041 cgtcgcgtac tccggctgtc gcagagcaag aaccgcgccc ggcggccaga aaacgccgca
     2101 agctgccaaa cgtcttacaa tggcgatact tggcggcagt tttgctgcta gccatcgttg
     2161 tttacctggc ttatggctac attcatacca agcaccagct agacaaactt tctaatcccc
     2221 aggtcgctac tcaagatgaa actcagcaat tagtaagtga agtcggtaaa cttgtgcagc
     2281 tgccatctgg tgaaaccccg gtgctcttta ccgttaatga cgcaagcaaa ctaaaaagct
     2341 tttcgttttt tcaagatgct cagaatgggg ataaggtgct agattaccct aaagcggata
     2401 aagctgtatt atatagaccg tctgcaaatc gtgtgatcgt ctacacgact gtcaacctca
     2461 ctaacgcccc tagtcaataa ttgtttacgc tagcctctcg ctcgtgatac ccttataggt
     2521 agaaaactca agagttaaac aaacatggcg aaaaagataa tcgcgatttt gaaccaaaag
     2581 ggcggggtgg gtaaaaccac cacggctatc aacctggcag cctacctggc taaggccggc
     2641 cataaggttc tgctgtgcga cattgacccg caggccaaca gcactagcgg cctgggcctg
     2701 gacaagcggg cgttaaactc gacattgtac gacgtactgt tttcgcgcgc ggaagcccat
     2761 aagactatcc gcaaagttac cagccacggc atctatttat tgccggctaa ccccgatctg
     2821 gccggcgccg aggtagaact ggtagaaata ccctaccgcg agctgaaact taaaagcgta
     2881 ctggacaatt tgaattacga ttatatcctg atagattgcc cgcccagctt ggggctgctg
     2941 acggtcaatg ccctggcggc cgccgacgaa gtgctgattc cggtacaggc cgagtactat
     3001 gccatggaag ggctaaccca gttactggca accatccagc aggtccgggc ggcgctcaac
     3061 ccgaacttga acatcctggg cgtggtggta acactgtacg ataaccgcaa ctcgctggcc
     3121 gaacaggtgc gggccgagct gcagaaccac tttggtgata aaatatttgt tacagtcata
     3181 ccgcgcaacg tccggctagc cgaagcgcca agctttggca agaccatcgt tgagcacgac
     3241 aaatggagca aaggcgcacg cgcttacaaa tcactggcaa aagaggtaga agagaggacc
     3301 aatggctaag aaacaggcac taggcagggg gtttgaagcc ttgattccca aggattttga
     3361 cgcctcgatg ttggaggaag acaaacaccg ggtgcagaaa atccttattc aagacatttc
     3421 gccgaatccg cagcagccca gaagggaaat ggatgaggcg gctttggcgg agctggcgga
     3481 atccatcaaa acccatggcg tactgcagcc gatcatcgtc gtcaaagccg gaaacggcta
     3541 tcagattgtc gcgggcgagc gtcgctggcg tgctgccaaa gccgccaagc taagccatat
     3601 gccggcaatc gtccgctcgc tcaaggaact ggagcggata gaactgtcgc ttatagaaaa
     3661 tatccaaaga gtggacctat cgccgttaga acaagcttta tctgtgttta agttacaaca
     3721 acaattcaat ctagcgctag aagacatcgc caaaaaactc ggtaaagccc ccagcaccat
     3781 aagcaatttg accagattac tacagttacc agagtctgcc cgacaagcgc taaaggacgg
     3841 caggatcagt gaaggtcat
//
LOCUS       DASPTF010000040         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1643961, whole genome shotgun sequence.
ACCESSION   DASPTF010000040 DASPTF010000000
VERSION     DASPTF010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643961"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..590)
                     /locus_tag="VEH48_01310"
     CDS             complement(21..590)
                     /locus_tag="VEH48_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXY18040.1"
                     /translation="MAFEDYKTKKAVVVVYTGEGKGKTSASVGLLARALGSGWRAAYI
                     QFIKTWDVGEHDFIYKIQPVFGKKLLFYKGGKGFYNAGDLSAKDVSEAEHKKAARRTY
                     NIAYKAATGGRFDIVICDEINNAVHHKLITKTDLKKLIKDKAPETSLCLTGRDFPQEL
                     LPLIDIATNMTKIKHHFDQKFLANKGIDF"
     gene            complement(617..1144)
                     /locus_tag="VEH48_01315"
     CDS             complement(617..1144)
                     /locus_tag="VEH48_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18041.1"
                     /translation="MGAEIPRENSSSSEDVQADAESSGANPGRGGEYDPAINPPDLSL
                     LNEIRYAVEAQTESSSPIDRNRFWARRVVNYARELADAPGARITPDDMRLLTTLSYTN
                     REESLRNLAAGLESDDLDFKSALEAILSAYETADTELKGIGDLRRFSLAFRRIGLMKN
                     AGRLAEVQGLASKRS"
     gene            complement(1210..1464)
                     /locus_tag="VEH48_01320"
     CDS             complement(1210..1464)
                     /locus_tag="VEH48_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HXY18042.1"
                     /translation="MSDKVGVTIYSASWCAFCHMARQYLTGLKVPFKDIDVEHDQDAA
                     RAIVQKTGQAGIPVIEIGDETIIGFDRPKIDLALRHYKLI"
     gene            1560..2207
                     /locus_tag="VEH48_01325"
     CDS             1560..2207
                     /locus_tag="VEH48_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HXY18043.1"
                     /translation="MNYNFNKRMAAKLGAELLGTGILAMMVLVMANTTAVSYFIATSA
                     AVTVAAIVMMFGAVSGAHINPAVTFGLWTARRIGTVKAVAYIVAQALGGLGAWLLYQY
                     FTNHSLASKTISFDTRLFIAEAVGAAIWVFGFTAVIARKAEALASAVALGTVVFASIM
                     IAATASLGYVNPAVAVAARSISVVYVVGPLVGGLVGANLYTMLFEGSAKKAAAKK"
     gene            complement(2255..>3088)
                     /locus_tag="VEH48_01330"
     CDS             complement(2255..>3088)
                     /locus_tag="VEH48_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXY18044.1"
                     /translation="LEQNLRWPDLRGGQEKDILKARLETYNLSVTHLLNFLDLTKGGP
                     EYFKQRNLLNLPEVKSASLSYGTAIHAAMDAAQKLTNRRAFSLAKVSAEFQAALRAEQ
                     LPAEQYKRYSSQGKRELLRLFKDLGYKLPKGSPSEQKFRDVMVGQARLGGKLDRVDNN
                     GERISIIDYKTGRPLKSFDTNDKSLQLKAYKHKLQLVFYSLLLGEQNLPVEGQMVYVE
                     AQNQRELLRTYQPTPEDIQRLKRMISVIWPMIINLNLPDTSAYSPDFDGIKSFEADLL
                     K"
BASE COUNT          676 a          778 c          818 g          816 t
ORIGIN      
        1 tttaaattgt tgtggataag ctagaaatca atgcctttgt tggcaaggaa cttttggtca
       61 aagtgatgct tgattttggt catgttggtg gcaatgtcga tcagcggcag aagttcttgg
      121 gggaagtcgc ggccggtcaa gcaaaggctg gtttcgggag ctttatcttt gatgagcttc
      181 ttgaggtcgg ttttagtaat cagtttgtgg tggacggcgt tgttgatttc gtcgcagatg
      241 acgatatcga atctaccgcc ggtggcggct ttgtaggcta tgttgtaggt tcggcgggcg
      301 gcctttttgt gctcggcctc gctgacatcc ttagccgaaa ggtcaccggc attgtaaaaa
      361 cctttaccgc ccttgtaaaa cagcaatttt ttgccaaaaa ccggctggat tttgtaaatg
      421 aaatcgtgct cgccgacatc ccaggttttg atgaactgga tataggccgc ccgccagccg
      481 ctgcccagcg cccgtgccag caggccgaca ctggcgctgg tcttgccctt cccctcgcct
      541 gtataaacga cgactacagc tttcttggtt ttataatcct caaacgccat cgggctttat
      601 tgtacaacgg acaaggctag ctgcgtttac tagccaaccc ctggacctca gctagtctgc
      661 cggcattttt catcaggccg atacgtctaa acgccaggct aaaacgcctg agatcaccga
      721 tgcctttgag ctcggtatca gcagtttcgt aagcactaag aattgcctcc agggctgatt
      781 tgaaatctaa atcgtcgctc tcaaggccgg ccgctaaatt cctcagactt tcttcacggt
      841 tagtgtaaga aagcgttgtc aataatctca tatcgtcagg cgtgatcctt gcccccggtg
      901 catctgcaag ctctcgtgcg taattaacca cccttctggc ccaaaaacgg tttctatcaa
      961 tcggcgatga agattcagtc tgcgcctcga cggcgtagcg gatctcattt aacaatgata
     1021 agtctggcgg gttgatcgct ggatcgtatt cgccgcccct acccgggttg gcgccgctcg
     1081 attctgcgtc agcttgaaca tcctcgcttg atgaactgtt ttctctcggt atttctgcgc
     1141 ccatgtcact agactataac accatatatg gtgttatggc aagcattagc ggcactatat
     1201 aacttatgat taaatcagct tatagtgacg gagagccagg tctatcttcg ggcggtcaaa
     1261 gccgataata gtctcatcgc cgatttctat aaccgggata ccggcctgac cggttttttg
     1321 cacgatggcc cgggcggcat cctggtcgtg ctcaacgtca atatctttaa agggcacttt
     1381 gaggccggtc aaatactgcc tggccatatg gcaaaaagcg caccatgatg cgctgtatat
     1441 agtaaccccg actttatcgc tcatcagatt aattatagca aattaaacag caatttggag
     1501 tttgggcaga ttagtaatat agtaactctc aggattaacc aaagggaggt ctaaggtaga
     1561 tgaattacaa ttttaacaaa aggatggccg ccaaacttgg cgccgagttg cttggaacgg
     1621 gcatcttggc gatgatggtg ctggtcatgg ccaacaccac ggcggtgtca tacttcattg
     1681 ccactagtgc ggctgtaact gtcgcggcga ttgtgatgat gttcggtgcg gtttccggcg
     1741 cccacatcaa cccggccgta acattcggtc tgtggacggc gcggcgcatc ggcacggtaa
     1801 aggccgtggc ctacatcgtg gcccaagctc tgggcggttt aggtgcctgg ctgctttacc
     1861 agtacttcac caaccatagc ttagcctcta agacgataag ctttgatact cggttgttca
     1921 tcgccgaggc cgttggtgcg gccatctggg tgttcggctt cacggcggtt atcgcccgaa
     1981 aggctgaagc cctggcctca gccgtggctc ttggaacggt agtgtttgcg tcaatcatga
     2041 ttgccgccac cgcttcattg ggttacgtca acccggcagt ggctgtcgcg gcccgcagca
     2101 tcagcgtagt ctatgtggtc ggcccgctgg tcggcggttt ggtcggagcc aacctctaca
     2161 ccatgttgtt tgagggcagc gccaaaaaag ccgcggccaa aaaataaaca cctaaacaga
     2221 ttcttgggaa gcgcccggca tcagctgggc gttttcattt gagcagatcg gcttcaaagg
     2281 acttgatgcc gtcaaaatcc ggcgaataag ccgaagtgtc cggaagattg aggttgataa
     2341 tcatcggcca gattaccgag atcatacgct taaggcgctg gatatcttct ggcgtcggct
     2401 gatatgtgcg cagcaactcc cgctggttct gagcttccac ataaaccatc tggccctcta
     2461 cgggcaggtt ctgttctccg agcagcaagg agtaaaacac cagttgcagt ttgtgcttgt
     2521 aagcttttag ctgtaacgat ttatcgttgg tgtcaaagct ctttaggggc cggccggttt
     2581 tataatcgat aatactgatt cgctcaccgt tgttatctac gcggtcaagc ttgccgccca
     2641 agcgggcttg gccgaccatg acgtcccgga acttctgctc gctgggactg cccttaggca
     2701 atttgtatcc cagatcttta aaaagccgta ataattccct tttgccctgg gagctataac
     2761 gcttgtattg ctcagccggc agctgttcag cccgtaaagc ggcttggaac tcagctgaga
     2821 ctttagccag agaaaaagcc ctgcggttag tcagtttctg agcggcgtcc atggcggcgt
     2881 ggatagcggt gccataagat aagctggccg atttgacctc gggcagattg agcaggttac
     2941 gttgtttaaa gtactctggt ccgcccttgg tcaggtcgag aaagttcaat agatgagtta
     3001 cgcttaggtt gtaagtctcc agccgggctt ttaagatatc tttttcctgg ccgccgcgca
     3061 ggtcgggcca acgcaagttt tgctccag
//
LOCUS       DASPTF010000041         8717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1074076, whole genome shotgun sequence.
ACCESSION   DASPTF010000041 DASPTF010000000
VERSION     DASPTF010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074076"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VEH48_01335"
     CDS             <1..294
                     /locus_tag="VEH48_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18045.1"
                     /translation="LEGNVAAFATQYFEMEAVDIYVPTNANATYVPLAVRRVLRQSWP
                     RFDVRRDQFWFSQDGFPLARTPEQAADPANYQRPMTVFSGLVRLVSELHKLNR"
     gene            446..3109
                     /gene="polA"
                     /locus_tag="VEH48_01340"
     CDS             446..3109
                     /gene="polA"
                     /locus_tag="VEH48_01340"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HXY18046.1"
                     /translation="MAEARKKLAIIDGKSVFYRGYYALPNLSTKEGVPTGGVFGFATM
                     AMELLKKLQPDYVAVAWDKPKTNIRKRLQMYPEYKAGRKPAPPDFYTQIPILHELLEA
                     FGWPLYELDDYEADDIMGTLAVQASKKGVETMLITSDLDALQLVNGHTKVYALKRGFS
                     NIEEFHPESFRAKYGLEPEQFLDLKALKGDSSDNIPGVPGVGEKTAIELLKDYKTLDG
                     VYENLPKIKESLRKKLEAGKKLAILSKKLAQIWTDAPMKLNLSEMDGRHIDAERLRAL
                     LQKLEFRTLLNNLPANMQTVIPKEDRVGGSKMKVGKNTVVASDKDLPKLGDSTWFYIH
                     SRSAGRHGKKPEVLIMSLDGKDTYTLDLRKLDQALIASHFKDVRPLVGYDVKSTLKML
                     MEMGVKDLPPVGHDVLVAAFLINSLLRQQTLTELATAELGYEGSSLDDLDTDELIARA
                     AEVMAVIRQLHEAQLKALEQTPKVNKLAKDIEWPLTPVLADMEYTGIQLDTGYLKKFN
                     RQLEDDISDLEQNIYGYADHEFNIGSPSQLADVLFVKLNLPKEGVKKGKTGYSTAANE
                     LAKLRGLHPIIDLITQYREVTKLKNTYVDTLPAQVDENSRLHTTFNLTVAPTGRLSSA
                     DPNLQNIPVKTDLGKNIRTAFVAGKDNVFVSADYSQFELRLAAALSGDKNMIDAFNRD
                     ADIHVETAAQLYDVPPDKVTKAQRSSVKAVNFGIMYGLGPHALSEQTGMSFGEARDFI
                     TRYFEIRPKLKAYIEKTREMTEKQGYVETLLGRRRPTPDVKSSNFQVREAAYRAAVNM
                     PLQGSAADIMKLAMIKVYEHFNQNGEWRMENEGSKPKILLQIHDSLLIECKKEDATAV
                     GKMVKQQMETAYKLPVKLTADISTGPNWGDL"
     gene            3106..3696
                     /locus_tag="VEH48_01345"
     CDS             3106..3696
                     /locus_tag="VEH48_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXY18047.1"
                     /translation="MNADLNTLLDFVKFTHEIRKIERTIYLEDDERQENDMEHSYQLA
                     LTTWFLIENDKLPLDKFRCVGMALLHDAIEVYAGDVSAHLPKYDSAKRKTDEKQAVQK
                     LKRRWPTFSSLHDLVDEYEACQTPEAKFVYALDKLIPVLNIYLYEGRSWHLMDISLQE
                     MKNIKVGKVDISKEIKDYYQQMVAVLEKQPQLFGKT"
     gene            3693..4100
                     /locus_tag="VEH48_01350"
     CDS             3693..4100
                     /locus_tag="VEH48_01350"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXY18048.1"
                     /translation="MIRLAGCVITDEKGRVLLLHRNNGKHSQWEIPGGKIDDGESAKQ
                     AAKREIKEELGVDVAIQEKIGEKEFQMDGKDFHYTWFTAKITDGKPKIAEPETFDNLK
                     YFPPGQMRQIYDELSLNTQNFLNAQGGGNLRPA"
     gene            complement(4168..5532)
                     /locus_tag="VEH48_01355"
     CDS             complement(4168..5532)
                     /locus_tag="VEH48_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18049.1"
                     /translation="MGRGSESRPNLTGSEPETVRLHKEALAALESWRQPLRFRDARSE
                     AAYDVVWGLLANRIDAVLQEHGLYEPADFSVLMEKDAAEENVRNKKKKKPPQRLVNVE
                     DVNNLAGWQDGIRQTLDPESSDILEPAYNSLLSKLRSEPAMAVSLAEEGEREVRTIKI
                     FISDTGARVEGSEKEISFIYNKTVDYSRERLWTLEYFADNPQAVIKFGDLWTRVLGMA
                     LEPNKHQERIIFSWLAQFTVDDDDETRQLFKIENKENQPKEAEISLNSPFRIVLERPQ
                     AAEYATKTFEQKLAMFDLTPAELHRAGSYLFSNRMVNSASFKKGPEGQVGLELFYYYD
                     SSARRLKEEDIRALEVYEEPVEIKAGESQESYENRRKRLAEASAEKLIKLIDDRKEFE
                     RFLEVMRPKPDEPIFGIYNFIQQFKESHPRYQLGRAFNLWMENHRNLRQVIRTTRSSR
                     TYSL"
     gene            5635..6450
                     /locus_tag="VEH48_01360"
     CDS             5635..6450
                     /locus_tag="VEH48_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXY18050.1"
                     /translation="MDVPGLKMNDELRIPQVGLGLWNIKGQSEMANAYNWALEAGYRL
                     FDSAQAYGNEEVLGRAVSAGKVPREEVFITTKISTSNMGHGLPESFDESLKKLRMDYV
                     DLLLLHFPMTITRRISWPLMEQIYASGRAKSIGVSNYTIKHLEELLAGCKVKPAVNQV
                     ELSVFLQQPELLDFCKQQGIVVEAYSPLTRSQRVNDPVLVEIAKKHDKTPAQVMIRWC
                     IEAGTVPIPKSSHHDRIIENIGVFDFELDADDMKQIAGLEEDFRTGWDPTHVE"
     gene            6455..6844
                     /locus_tag="VEH48_01365"
     CDS             6455..6844
                     /locus_tag="VEH48_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HXY18051.1"
                     /translation="MKTRTFKLNKLVRDKIVQSTEAQGGTVKYRTLQGKNLTEVLLTK
                     LIEEANELKSSDLKVGELADLQELVDALAKHLGMPKRELSKRQSEKRRKNGAFKKGHF
                     IDTVTLPANNKWAKYYAADPNRFPEIN"
     gene            complement(6877..7551)
                     /locus_tag="VEH48_01370"
     CDS             complement(6877..7551)
                     /locus_tag="VEH48_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18052.1"
                     /translation="MSDSRVEVIGQDEFNPREWLRRELLWASLMAPYRDQLDGWLDGR
                     AIAQHDGNVTRRDLYDIDGDTEAKALAFFNQGKNIAGIISSAILLRAGADGTIEKHPA
                     DMLEVQANGVDRVLGRIAANEVIIIEIPEDRAYEVCSRLVHIAMAGIDRQDHLTYASL
                     IMTELAIAETKSWLDTARQNEAGSLPILKISDGGYPRSAGARVDKLQPVFHKIIESEP
                     YQDLAA"
     gene            complement(7672..>8717)
                     /locus_tag="VEH48_01375"
     CDS             complement(7672..>8717)
                     /locus_tag="VEH48_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HXY18053.1"
                     /translation="WLEKIKHKDAEALRKEDNDKRERLQVLKDIIGLPYDKQVKFEAT
                     DLRDRTPRFVKYEKEHGNELCALRLIPKDHSLPKLRMRGKTVAKVYDWFLEQSVDSNK
                     YRAEYIPHAEVSDWSTIFIVNKHGMQGEIIPGGHYQLTQGFFDSVKPIVFSFDFKDWR
                     LSRDNPEAKKHLKSIARHIFVKDAAKRTLIRRDLRGDFASGYLTGYWETVSSKEFGLW
                     FVDYSRVLGEMYGDMQITPAVQKPGRVSGLSGSSGKAKGPVKIVQADKIGQGFPDGGV
                     LVCEVTTPNYVPLMQKAAAIVTDQGGILSHAAIVARELKKPCIVGTGNATRVLKNGQI
                     VSVDANVGLVKPA"
BASE COUNT         2273 a         2312 c         2124 g         2008 t
ORIGIN      
        1 ctcgagggca atgtcgcggc cttcgcgact cagtacttcg agatggaggc ggtggacatc
       61 tacgtcccta ccaacgccaa cgcgacatac gtaccgctcg ctgtgcggcg cgtcctgcgc
      121 caatcctggc cgagattcga cgtccggcgc gaccagttct ggttcagcca ggacggcttc
      181 cccctcgcgc gtacgcccga gcaggcagcc gacccggcca actaccagag gccgatgacc
      241 gtcttcagcg gcctggtgcg gctcgtcagt gagctgcaca agctcaaccg ctagtctctt
      301 cgttcggcga agagaaggaa aggcggcatg tccatgattc cttgttcgct caacaggcct
      361 gtggcgcttc ggcgctgcag gcctctttcc ttttataaac cataagcttt cgcggtttta
      421 acagatgcgg cgtataatct agcctatggc ggaggcgcgg aaaaagctgg cgattattga
      481 cggtaaaagt gtcttttacc ggggttatta cgcgctgccc aatttatcta ccaaagaagg
      541 tgttcccacc ggcggcgtgt tcggctttgc caccatggcc atggagctgc tcaaaaaact
      601 ccagccggac tacgtggccg tagcttggga taaacccaaa accaacatcc gcaagcgcct
      661 gcagatgtat cccgagtaca aagccgggcg caagccggcg ccgccggact tttacaccca
      721 gatcccgatt ttgcacgagc ttttggaagc ttttggctgg ccgctttatg agctggacga
      781 ttacgaagcc gacgatatca tgggcacgct ggcggtgcag gccagcaaaa agggcgtgga
      841 aaccatgctc atcacctctg atttggatgc actgcagctg gttaacggcc acacaaaggt
      901 ttacgctctc aagcgcggtt tttccaacat tgaagagttc cacccggaaa gtttcagggc
      961 caaatacggc ctggagccgg agcagttcct ggacctaaag gcattgaaag gcgacagcag
     1021 cgacaacatc cccggcgttc cgggcgtggg cgaaaagacc gccattgagc tgttaaaaga
     1081 ctacaaaacg ctggacggtg tctatgagaa tctgcccaaa atcaaagaat ctttgcgcaa
     1141 aaagttagaa gccggcaaaa agctggctat cttgagcaaa aaactggcgc agatttggac
     1201 cgacgcaccc atgaagctca atttatcgga gatggacggc cggcatattg acgccgaacg
     1261 cctgcgcgcg ctgctgcaaa aactggagtt ccgcacgctc ttaaacaacc tgccggccaa
     1321 catgcaaacc gttataccca aagaagaccg cgttggcggc agcaagatga aagtcgggaa
     1381 aaacactgtt gttgccagtg acaaagacct gcccaagctg ggcgattcta cgtggtttta
     1441 catccatagc cgctcggctg gcagacacgg caaaaaaccc gaggtcttaa taatgagctt
     1501 ggatggtaaa gacacttata ccctggacct gcgcaagctg gaccaggcat taattgccag
     1561 ccattttaaa gatgtacggc cgttggtggg ctacgatgtt aaatcaaccc taaaaatgtt
     1621 gatggaaatg ggcgtcaaag acctgccgcc ggtcggccac gacgtgctgg tcgcggcttt
     1681 tctaataaat tccttactgc gccagcagac tctaactgaa ctggcgactg cggagcttgg
     1741 ctacgaaggc tcttcacttg atgacctgga tacggacgag ttgattgccc gcgcggcaga
     1801 agtcatggct gtaatccgcc agttgcatga agcccaactt aaagccctgg aacaaacgcc
     1861 caaagttaac aaactggcca aagatattga atggccgctc acaccggtgc tggccgacat
     1921 ggaatacacc ggcatccagc tggacaccgg ctacctcaaa aaattcaacc gccagttaga
     1981 agacgacatt tccgaccttg aacaaaacat ttacggctac gccgaccacg aattcaacat
     2041 cggcagtccc agccagctgg ccgacgtgct gttcgtcaag ctcaacctgc caaaagaagg
     2101 tgtaaaaaag ggtaagaccg gctacagcac ggccgccaac gagctggcca aactacgcgg
     2161 cctgcacccc atcattgacc tcatcaccca gtaccgcgag gtcaccaagc tcaaaaatac
     2221 ctacgtggac acgctgccgg cgcaggtgga cgaaaacagc cgcctgcaca ccacttttaa
     2281 cctgaccgtg gcgccgaccg gccgcttaag cagcgccgac cccaacctgc aaaatatccc
     2341 ggtcaaaacc gatttgggca aaaatatccg cacggccttc gtggcgggca aggacaacgt
     2401 attcgtcagc gccgattata gccagtttga gctgcgcctg gcggcggctt tgtccggcga
     2461 caagaatatg atagacgcct ttaaccgcga cgccgacatc cacgttgaga ccgccgccca
     2521 gctttatgac gtgccgccgg acaaagtcac caaagcccaa cgctcatctg ttaaagccgt
     2581 gaattttggc attatgtacg ggctgggccc gcacgcgctc agcgagcaga ccggcatgag
     2641 ctttggcgag gcccgcgact ttataacccg ctattttgaa atccgcccca agctcaaagc
     2701 ctacatagaa aaaacccgcg aaatgactga aaagcaaggc tatgtggaaa cactgctggg
     2761 gcggcggcgc cccacgccgg atgtcaaatc gtccaacttc caggtgcgcg aggccgccta
     2821 ccgcgcggcc gtaaacatgc ctctgcaagg cagcgccgcc gacatcatga agctggccat
     2881 gattaaagtg tatgaacact ttaatcagaa tggagaatgg agaatggaga atgaagggag
     2941 taaaccaaag atacttttgc agattcacga ctcccttcta atcgagtgca aaaaagagga
     3001 cgccacggct gtgggcaaaa tggtcaaaca acagatggaa accgcctaca aactaccggt
     3061 caaactgacg gccgacattt ccaccggccc caactggggc gacctatgaa tgcggatttg
     3121 aatacactgt tggacttcgt aaaattcact cacgaaatta ggaagattga acgaactatc
     3181 tatcttgaag atgatgagcg ccaggaaaat gacatggagc acagctacca actggcgctc
     3241 actacctggt ttttgattga aaacgacaaa ctgccgctgg ataaattccg ctgcgtgggt
     3301 atggcgctgc ttcatgatgc tattgaagtt tacgccggcg acgtatcggc tcacttacca
     3361 aagtacgact cggcaaagcg taaaactgac gagaagcagg ccgtccaaaa actcaaaaga
     3421 cgctggccga cattcagctc gctgcatgat ttggtagatg aatatgaagc ctgccagacc
     3481 cccgaggcaa agttcgtcta tgccctggat aaactcatcc ctgtacttaa tatctatctt
     3541 tacgaaggcc ggtcctggca tttgatggat atctcgttgc aggagatgaa gaatataaaa
     3601 gtcggtaagg tcgacatctc caaagagatt aaagactatt accaacaaat ggtcgcggtt
     3661 ctagaaaaac agccccaact cttcggcaaa acatgatcag gctggcgggg tgcgttatta
     3721 cagatgaaaa gggcagggtt ttgctgctgc accgcaataa cggcaaacac tcgcagtggg
     3781 aaatcccggg cggcaagatt gatgatggcg aaagcgccaa acaagcggcc aagcgcgaaa
     3841 tcaaagaaga gttaggcgtt gatgtcgcta tccaagaaaa aatcggcgaa aaagaattcc
     3901 aaatggacgg caaagatttc cactacacct ggttcacagc caaaataaca gatggtaagc
     3961 caaaaattgc cgagccagaa acttttgaca acctcaaata ttttccgccc gggcaaatgc
     4021 gccaaattta tgacgaacta tcgctcaaca cccaaaattt ccttaatgct cagggcggcg
     4081 gaaacctccg accagcttag aaagtataat taagaagcga agaggcgact acggcgcgat
     4141 tgctgaggcc tagatacgca caatgagcta aagcgagtac gtacgagagg aacgggtcgt
     4201 ccggattacc tggcgcagat tcctatggtt ctccatccat agattgaacg ctctgccaag
     4261 ttggtaccgg ggatggcttt ctttgaattg ctgtataaaa ttatagatgc cgaaaatcgg
     4321 ctcatcgggc ttggggcgca ttacctctag gaaacgctcg aattccttcc ggtcgtcaat
     4381 aagcttaatt aatttttcag ccgacgcttc ggccagcctt ttccgacggt tctcgtaaga
     4441 ttcctggctt tcgccggctt ttatctcgac cggctcctca tatacttcca gcgccctgat
     4501 atcctcttcc ttcaatctcc gcgccgagct atcgtaatag taaaaaagtt ccagtcctac
     4561 ttggccctcc gggccctttt taaacgatgc actgttaacc atcctgttgc taaacaagta
     4621 gctaccggct ctatgcagtt ccgccggtgt caggtcaaac atggctaatt tttgttcgaa
     4681 ggtcttagtc gcatactcgg cagcctgggg cctttcaagg actatcctga acggactgtt
     4741 cagactgatc tcagcctctt taggttgatt ttccttattc tcgattttaa atagctgcct
     4801 ggtctcatca tcgtcgtcga ccgtaaactg ggccagccag ctgaatatga ttctctcctg
     4861 gtgtttgttg ggttctaaag ccattcctag aacacgggtc cataaatcgc caaatttgat
     4921 tacagcctgc ggattatctg caaagtattc aagagtccac agccgttcac gggaatagtc
     4981 gactgttttg ttatagataa aagagatttc tttctcactg ccctcgaccc gcgcgccggt
     5041 atccgatata aatattttga tagttctcac ttctcgttct ccctcctcag ccaagctgac
     5101 agccatagcc ggttcgctcc ttaacttaga cagcagcgaa ttgtaggccg gctccagtat
     5161 atctgaagac tccgggtcca gtgtctgccg aataccatcc tgccaaccgg ccagattatt
     5221 aacatcttca acattaacca gccgttgtgg cggctttttc tttttcttat tccgaacatt
     5281 ctcttcagcg gcatcctttt ccatcaacac gctaaaatca gccggctcgt acagcccgtg
     5341 ctcttgcaga accgcatcaa tcctattggc taataggccc cacacgacat cgtaagcggc
     5401 ttcagaacga gcatcacgga atcgtagcgg ctgacgccaa ctctccagcg ccgccaacgc
     5461 ttctttatgc aggcgtacag tttccggctc gctaccggtt aaattcggcc tggattcaga
     5521 gccccgtccc ataaaaaata ctacctcctt ggtagatcca agaccacatt atcatatttc
     5581 atgcaataat aacagattgg cgagtataat agatggtgta actaagatgt atttatggat
     5641 gtcccgggtt tgaagatgaa tgatgagttg cggattccgc aggttgggct ggggctttgg
     5701 aatatcaagg gccaaagcga aatggcaaat gcttataact gggcgctgga ggccggctac
     5761 cggctgtttg attcggcgca ggcctacggc aacgaagagg ttttaggccg ggctgtcagc
     5821 gccggcaaag tcccgcgcga agaagttttt atcaccacca aaatcagcac tagcaatatg
     5881 ggccacggct tgccggaaag ttttgatgaa tcgctcaaaa aactgcggat ggattatgtg
     5941 gatttgctgc tgctgcactt cccaatgacc atcacccgcc gcatatcctg gccgctgatg
     6001 gagcagattt atgccagcgg ccgagctaaa tccataggcg tcagcaacta cactatcaaa
     6061 catctggaag agcttttggc cggctgcaaa gttaaaccgg cggtcaacca ggtggagctg
     6121 agcgttttcc tgcagcagcc ggaactgctg gatttttgta aacaacaggg aatagttgtt
     6181 gaagcctaca gcccgctgac ccgctcgcag agagtcaacg accccgtgct ggtagaaatt
     6241 gccaaaaagc acgacaaaac tccggcgcag gtgatgatcc gctggtgcat agaggccggc
     6301 acagtaccga tccccaaatc gtcgcaccac gacaggatca tagagaacat cggcgttttt
     6361 gactttgagc tggacgccga tgacatgaaa caaatcgccg gcctggaaga agatttccgt
     6421 accggctggg accccaccca tgtagaataa gagcatgaaa acccgcactt ttaaactcaa
     6481 caaactggtg cgggacaaaa tcgtccaaag cacagaagca caaggcggaa ctgtcaaata
     6541 tcgcactctt caaggtaaaa atctcacgga agttttattg accaaactta tagaagaggc
     6601 gaatgagcta aaatccagcg acctcaaggt tggcgagcta gccgatttac aagaactggt
     6661 tgatgcgctg gccaagcact tgggcatgcc aaaacgtgaa ttatctaagc gccaatctga
     6721 gaaacgccgc aaaaatggag ctttcaaaaa aggccatttt atagatacgg ttacgctgcc
     6781 cgcaaataat aaatgggcta aatactacgc cgccgacccc aaccgctttc ctgaaattaa
     6841 ttgacgacgc ccgtattgac tgaataaatc aactaattaa gctgcgaggt cttggtaagg
     6901 ttcggattcg ataattttat gaaagaccgg ctgtagctta tctactcgtg ccccagcaga
     6961 gcgcggatag ccgccatctg agatttttaa tattggcagg gagcctgcct cgttttgtcg
     7021 tgccgtatcc aaccaagatt tagtctcggc gatagccaat tctgtcatga tcaggctggc
     7081 gtatgttagg tgatcttgcc tatcaattcc tgccatcgcg atatgtacca gccgtgaaca
     7141 tacttcataa gctctatctt caggtatttc tataattatg acttcgttag cagctatacg
     7201 acctaatact ctgtcgacac catttgcctg gacttctagc atgtcagccg ggtgtttttc
     7261 gatggttcca tcggccccag ctctaagtag tattgccgac gaaattattc ccgcaatatt
     7321 tttaccctga ttgaagaagg caagggcctt tgcttctgtg tcaccatcaa tatcatatag
     7381 atcccgcctg gtaacattac catcgtgctg cgctatggct cttccatcca accaaccgtc
     7441 cagctgatct ctgtagggtg ccattagtga ggcccaaagc agctctctgc gcagccactc
     7501 acggggattg aattcgtctt ggccaataac ctcaactcga ctatcagaca taagtttgag
     7561 tattataata taaacacagg ttattctcaa ggtgttgaat aggttacgcg gtttaccgat
     7621 actaaagata atacttggga ggcatctagg gtattgcttt gggttagtaa ctcaggctgg
     7681 cttaacaaga ccaacgttag catcaacaga tacgatttgg ccgtttttaa gaactctggt
     7741 ggcgttgccc gtacccacga tgcagggctt tttaagctcg cgagcaacga tggcggcgtg
     7801 cgataaaatg ccgccttggt ccgtgacaat ggccgcggcc ttttgcatca gcggcacgta
     7861 attgggtgtc gtgacctcac agaccagcac gccgccatca ggaaagccct ggccgatttt
     7921 gtccgcttgg acaattttta ccggcccttt agccttgccg gaactgccgc tcaggccaga
     7981 aaccctgcct ggtttttgaa ccgcaggcgt aatttgcata tcgccataca tttcacccag
     8041 aacgcggctg taatccacaa accacaaacc gaattccttg ctggaaactg tctcccagta
     8101 gcccgtgagg taaccgctgg caaaatctcc tctcagatct cgccggataa gcgtacgctt
     8161 ggcggcatct tttacaaaaa tgtgcctggc aatcgattta aggtgttttt tggcttccgg
     8221 attgtctctg gaaagacgcc aatctttaaa atcgaagcta aatacgatag gtttaaccga
     8281 gtcaaagaag ccctgcgtca gctgatagtg accgcccggg ataatctcgc cctgcatccc
     8341 gtgcttatta acaataaaaa tagtggacca atccgatacc tcggcgtgcg gaatgtattc
     8401 ggcgcggtat ttgtttgagt caacgctctg ctctaaaaac caatcataaa ctttggcgac
     8461 cgttttacca cgcatcctta gcttgggcag agagtgatct ttaggaatca gccgcagggc
     8521 acacagttcg ttgccgtgtt ccttttcgta tttaacaaac ctgggcgtcc ggtcgcgtaa
     8581 atcggtcgct tcaaacttga cttgcttgtc gtatggcagg ccaatgatgt cttttagtac
     8641 ttgtagacgt tcgcgcttgt cgttatcttc ttttctgagc gcctcggcgt ctttgtgctt
     8701 gattttttcc agccatt
//
LOCUS       DASPTF010000042         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1650275, whole genome shotgun sequence.
ACCESSION   DASPTF010000042 DASPTF010000000
VERSION     DASPTF010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650275"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..988
                     /locus_tag="VEH48_01380"
     CDS             284..988
                     /locus_tag="VEH48_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18054.1"
                     /translation="MRGESFRGSIDPVPEAGRDPEVEELLEFLDIYRKIQELLAWLIE
                     ASANSDTVIVGIRRARLSDLMQQLGLDPENPQRLTELEEINNELFNRIRRPQPRVDMP
                     EEDFFASRIKEIVAERAPQHLEYVSDINVNLVQFAEHGHTDWGTIASRTGAISLAAYT
                     RGLAQIMADPIAYHAEFGGLGIDPSRRAPDHLAVNLEWVIANGRHRALAIRSLGPEYV
                     AESDMSQWVPVIIEEM"
     gene            1061..2407
                     /gene="radA"
                     /locus_tag="VEH48_01385"
     CDS             1061..2407
                     /gene="radA"
                     /locus_tag="VEH48_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HXY18055.1"
                     /translation="MAKSLGVKYVCSNCGQGFSSWTGRCSSCGEWNTLQEQIEISTAT
                     AGASGKSLRTESVAAAAKTRMDRIGCGISEVDEVLGGGIVAGSVNLLAGQPGIGKSTL
                     LLQIAYNISAKHKVLYVSGEESARQIALRAERLGVANKALQLAVSNVANDIASTVSAG
                     GYDVVVVDSVQAVSVNEISSAAGTVSQITNSTQLLSAAAKSSNTALLLVGHVTKEGSI
                     AGPKVLEHIVDVVLQLEGDRYGGFKVLRAAKNRYGSTNEAGIFEMTDKGMQAVANPSA
                     ALLAERQVSDGSIVTATMEGTRPVLVEVQALVNTTSYGYPKRAASGIDLNRVNLLVAM
                     LERRTKLKLADKDIYVNIVGGIRLSEPAADLAVCMAIGSAAKGLQLKKNAVVFGEVGL
                     SGEVRHVPYLEKRIAEAKKLGFEAAIGPKVRSGKAPALLLPVTDVRSALNTFLEKD"
BASE COUNT          600 a          603 c          712 g          537 t
ORIGIN      
        1 aagagtatcg gctatatcaa aaaaaagctc acccactttc ggcagtctat cggcgtgaaa
       61 tatgtaggct tcacgagtca tatttgcaca acgataacat gaaaaataaa aatcagcaat
      121 gtagcgggaa ctagcaatgt cccaagtaca gagagggggt gtttgacttg ttgagtattt
      181 ttgacagatg ggttttggcg ggtttgcccc tgtgattgta ttatcattga acttatggta
      241 tagtaatcat aagttttaga gtttaaacat cacagttgaa attatgcgag gtgaaagttt
      301 tagaggatcg atagatcccg taccagaagc tggacgtgac ccagaagttg aagagctttt
      361 ggaattcctg gatatttatc gaaaaatcca agagttattg gcatggctca tagaagccag
      421 tgcgaactcc gacacagtta tcgtaggcat caggagagcc aggctgagtg atttaatgca
      481 gcaattagga ttagacccag aaaacccgca aagattaact gagcttgaag aaataaataa
      541 tgaacttttt aacagaatcc gtcgccccca accccgagta gatatgcccg aggaagactt
      601 ctttgcttcg cgtatcaagg aaatcgttgc ggagcgcgca ccccagcacc tcgagtacgt
      661 cagtgacatt aatgtgaact tagttcagtt tgctgaacac ggtcacaccg attgggggac
      721 tattgcgtca cgaactggcg ctatatccct ggccgcctat acgcggggat tggctcaaat
      781 tatggcagac ccgatagctt atcatgccga gttcggcggg cttgggattg acccgtcaag
      841 gcgtgcccct gatcacttag cggtaaatct ggaatgggtc atagccaatg gcaggcaccg
      901 tgcattagca atcaggagcc ttgggccgga gtatgttgca gaatcggaca tgtctcaatg
      961 ggtgccagtg attattgaag aaatgtgatt aacatcgcgt agttaagctc ttggtaccaa
     1021 gaaaacggat gggtttgggc gggtttgcta tgattgagtc atggctaaga gtttgggagt
     1081 caagtatgtg tgctccaatt gcgggcaggg attcagcagc tggaccggac gctgcagcag
     1141 ctgcggcgag tggaacaccc tgcaggaaca gatagaaatc agcaccgcca cggctggtgc
     1201 cagcggcaag agcctgcgca ccgagtcggt tgcggccgcc gccaaaacca ggatggaccg
     1261 catcggttgc ggcatcagcg aagtggatga ggttttgggc ggcggcatag tcgccggcag
     1321 tgtcaacctg ctggccggcc agcctggtat cggcaaaagc acactgttgc tgcagatcgc
     1381 ttataacatt tcagccaagc acaaagtact gtatgtcagc ggcgaggaat cagcccgtca
     1441 gatcgccctg cgcgccgaac gcctgggtgt ggccaacaaa gctttgcaat tggctgtatc
     1501 caatgtggcc aatgacatcg ccagcaccgt ctcggccggt ggctacgacg tcgtggtcgt
     1561 ggattccgtc caggccgtat ctgttaatga gatatcttcg gccgccggta ctgtcagcca
     1621 gattactaac agcacacagc ttttatccgc ggctgccaaa tccagcaaca ccgctctgct
     1681 gctggtcgga catgttacca aagaaggttc aatcgccggt cccaaggtgc tggagcacat
     1741 cgtggacgtg gtgctgcagc tggaaggcga ccgttacggc ggcttcaagg ttctgcgggc
     1801 ggccaaaaac cgctacggct ccaccaatga ggccggcatt tttgagatga ccgacaaggg
     1861 catgcaggcg gtagccaacc cgtcggcggc gctgctggcc gagcgccagg tcagcgacgg
     1921 gtccatcgtt accgccacca tggaaggcac ccggccggtg ctggtggaag tccaggcgct
     1981 ggtcaacact actagctatg gttacccaaa gcgggcggcc agcgggattg acctcaaccg
     2041 ggtgaatctg ctggtggcaa tgctggaacg ccgtaccaaa ttaaagctgg ctgacaaaga
     2101 catttatgtg aacatcgttg gcggcattcg gctcagtgag ccggcggcgg acctcgccgt
     2161 ctgcatggcc atcggcagcg ccgctaaagg cctgcagctg aagaaaaacg ctgtagtctt
     2221 tggcgaggtc ggcctgtccg gcgaggtccg ccacgtgccg tacttagaaa agcgtatcgc
     2281 cgaggctaaa aaactgggct ttgaggcagc tatcggcccg aaagtgcgca gcggcaaggc
     2341 gccggctctt ttgctgccgg taacggatgt gcgcagcgca ctcaacactt tcttagagaa
     2401 agattagggt ctgattacgg tcacttggtc gctgtcgcct gaaccgtcgt tg
//
LOCUS       DASPTF010000043         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_2080998, whole genome shotgun sequence.
ACCESSION   DASPTF010000043 DASPTF010000000
VERSION     DASPTF010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080998"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VEH48_01390"
     CDS             complement(<1..456)
                     /locus_tag="VEH48_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXY18056.1"
                     /translation="MIDSLRLQNFRSYADASFEFEPGVNIVVGPNASGKTNLLEAVLT
                     LARGSSFRARDSELVRFGKPWTRLDGYFGNQARTLKLIFQGGALETGQGPNKSFLIDD
                     KPLRRLSLERTVPAVLFEPNHLQLITRGPEQRREFIDDLLERSRPGFKTL"
     gene            complement(495..1586)
                     /gene="dnaN"
                     /locus_tag="VEH48_01395"
     CDS             complement(495..1586)
                     /gene="dnaN"
                     /locus_tag="VEH48_01395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXY18057.1"
                     /translation="MKLQVTQENLAKALNTTARVANTRNTLPILANVLIKTDKNRLAV
                     SATNLDIAISQTIGSKIEKDGAITVPARLMQDFVSSLPDGVLNLELVDNKLSITTDQY
                     QSTINGVAADDFPVMPAISGGSSWKVPAAGFKKALAQVVFAASADDARPVLTGVYFHR
                     LGGEVAAAATDSYRLAETRLARTKGGVNFLVPASAVADLLRIIDDTDKEVSVTHDDQQ
                     VQFQVGDVTLVARLIEGNYPDYRKLIPTKFATTAKLKKADFANIAKVSSLFARESAGS
                     ITIKVDKTAKEVSINAVASQLGENNAKASAAVSGSGEVTLNSRYLMDALNAFSSEDVE
                     FCFNGKLEPIILRASGEPSYLHLIMPLRS"
     gene            complement(1844..>2103)
                     /locus_tag="VEH48_01400"
     CDS             complement(1844..>2103)
                     /locus_tag="VEH48_01400"
                     /inference="COORDINATES: protein motif:HMM:NF019903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXY18058.1"
                     /translation="TAKHFHLSMEDILGPKRDKDIVVPRQIAMYMLRSELHLSFPKIA
                     RELGRKDHTTAIHSVEKIEKELGYDGPIKQYVGELKERLYA"
BASE COUNT          466 a          529 c          587 g          521 t
ORIGIN      
        1 caaagttttg aaccccgggc ggctgcgctc cagcaggtca tctatgaatt cccggcgctg
       61 ctccggaccg cgggtaataa gctgtaagtg gttgggttca aacagcacgg ccggcacggt
      121 gcgctccaag ctcaaccggc gtagcggctt gtcgtctatt aaaaagctct tattcggccc
      181 ctgacccgtt tccagggcgc caccctggaa aatgagtttt agtgttcggg cttgattgcc
      241 gaaatagccg tccagccggg tccagggctt gccgaaacgc accagttcgg agtcacgggc
      301 cctgaaagag ctaccgcggg ccagagtcaa aaccgcctcc agcaaattgg tcttgccgct
      361 ggcattcggc ccgaccacaa tatttacacc tggctcaaac tcaaaactgg catcggcgta
      421 tgaacggaaa ttttgtaaac gcaggctgtc aatcacaata atagggtata gggtttaggg
      481 gatagggtat agggttagct gcgcaagggc attatcaggt gcaggtaaga tggttcgccg
      541 ctggcgcgca gaataatcgg ctccaactta ccattaaaac aaaactccac gtcctcgctg
      601 gaaaatgcgt taagagcgtc cattaagtag cgagagttga gcgttacttc accggagccg
      661 ctgaccgcgg cgctggcttt agcgttattt tctcccagct gcgaggccac ggcgttgatg
      721 ctgacctctt tggcggtctt gtccactttg atggtgatac tgccggcgct ctcgcgggcg
      781 aataggctgg agactttggc gatattggca aagtcggctt ttttgagctt ggcggtagtg
      841 gcgaattttg tcgggataag tttacggtaa tcgggatagt tgccctcgat cagccgggcc
      901 accaaggtta cgtcgccgac ctggaactgc acctgttggt cgtcatgggt aacgctcact
      961 tctttgtcgg tatcatcgat gatgcgcagt aggtcggcga cagcgctggc cggcaccaga
     1021 aaattaacgc cgcctttagt tctggccagc cgggtttctg ccagccggta gctgtcggtg
     1081 gccgcggccg ccacttcacc gcccagccgg tgaaaataga cgccggtcag caccggacgg
     1141 gcgtcatcag cgctggcggc aaacaccacc tgggccaagg cttttttgaa accggcggcc
     1201 ggaaccttcc agctactgcc gccggagatt gccggcatca ccggaaaatc atcggcagcc
     1261 acaccgttaa tggtcgattg gtattggtcg gtggtgatac ttaatttgtt gtcaaccagc
     1321 tctagattca aaacgccgtc tggcaagctg ctgacgaaat cctgcatcag cctcgctggg
     1381 acagttatgg caccgtcttt ttcaatctta gagccgatgg tctgactgat ggcgatatcc
     1441 aagttggttg cagagacggc caagcggttc ttgtcagtct taatcaacac attagcgaga
     1501 atcggtagtg tattacgagt gttagctacg cgggcggtgg tgtttaaggc tttggctagg
     1561 ttttcctggg tgacttggag cttcatgtta attcctttat taatttagtt tagtagtaat
     1621 agtaggagcg ggggacacag tggataaagg atgtaataat agacaaaaat ctagtgtttt
     1681 agattgcgaa aaaatggtgc cgaactcagc caggcttgcg cacaaactgc gcataaaaaa
     1741 tacttgactt gttgagtatt ttgggctggc tgtggattgt gctgataact ccgggctttt
     1801 agcacagcct gtgcttaagg cctccacaaa ctgtccacag gccttaggca tacagccgct
     1861 ccttcagctc gccgacatat tgtttgatcg gtccgtcgta accaagttct ttctcaattt
     1921 tctctaccga gtggatggcg gtggtgtggt ctttacgacc caactcgcga gcaatcttcg
     1981 gaaagcttaa gtgcaactcg cttctaagca tatacatagc gatctgcctg gggaccacga
     2041 tgtctttgtc gcgcttaggc cccaagatat cctccattga aaggtggaaa tgcttggcgg
     2101 tgc
//
LOCUS       DASPTF010000044        15224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_211520, whole genome shotgun sequence.
ACCESSION   DASPTF010000044 DASPTF010000000
VERSION     DASPTF010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 15224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15224)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15224
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211520"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..683
                     /locus_tag="VEH48_01405"
     CDS             303..683
                     /locus_tag="VEH48_01405"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HXY18059.1"
                     /translation="MVEVTNNQFEEFIQEGIAAIPPLYRQRLQNVAFLLEDEPTPEQR
                     TKLNLYPHETLFGLYEGVPLPQRGGTTKILPDKITLFKKPLQNASRDLNDLKEAIRHT
                     VWHEVAHYYGLDHKRIHELDGTSS"
     gene            788..1090
                     /locus_tag="VEH48_01410"
     CDS             788..1090
                     /locus_tag="VEH48_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18060.1"
                     /translation="MQFSHKSSVAWMVHFFVGVAELLLALRVVLRFFNGNPDATFVHW
                     CYTSTAVLLEPFRNVFTSTGVVQRGWVVDYVALFAMAVYALTGYLALGLVNRWVKR"
     gene            1168..1932
                     /locus_tag="VEH48_01415"
     CDS             1168..1932
                     /locus_tag="VEH48_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXY18061.1"
                     /translation="MAYERSPAYKPGQKEPFKISRSKIDLFIQCPRCYWLDARLKISR
                     PSGPPFTLNSAVDQLLKQEFDSLRKAGQQHPLQIEYGIDARPVSHPKLNDWRHNFTGV
                     QALYKPANFLVFGAIDDLWQNSKGEFIVLDYKSTSKAGKITELGQESWHDQYRRQLGV
                     YQWLLRQNGLKVSNTGYWLYCNAIKNGKVFDNKLEFELTLIAEKIDDKWVEPTLAKIK
                     DCLEGEIPKESPDCEFCAYARSRTQLTLDSIKGARG"
     gene            complement(1929..2459)
                     /locus_tag="VEH48_01420"
     CDS             complement(1929..2459)
                     /locus_tag="VEH48_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXY18062.1"
                     /translation="MEIQKVFKFCPRCGSQFGNKDNLLKCPACGLSFYINPKPCTAVV
                     MINEKGEYLLVKRAVEPGKGLWDLPGGFVEENETFEENARREVYEELGIDLSNQLHYT
                     GSHTEPYSFQGVNYPTLAVCFVCKFPRGTKPKPADDVAEYKFFSPKSLPTESFAFEAM
                     KRDFADVALFLKKNRI"
     gene            complement(2479..3096)
                     /locus_tag="VEH48_01425"
     CDS             complement(2479..3096)
                     /locus_tag="VEH48_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18063.1"
                     /translation="MKKLKFQHPTNRVLIFLVVMLIGLLTAVIWDFGQKIDILWRQNI
                     ISANFYSSTGATTIDIKVYIQNAEKGLYSDQTVVDPIGERVYIPEARIYLPLTDLSRN
                     LLYNYEAADSTQNTPAQVSINTSFNINRLVNTFADVPCAERMAGFSITKKDGSLVGNH
                     FSGSMVLQDGRTLYLFTNVTDSCANYWGPDNPDAIVNLLKQAQSY"
     gene            3177..4481
                     /gene="serS"
                     /locus_tag="VEH48_01430"
     CDS             3177..4481
                     /gene="serS"
                     /locus_tag="VEH48_01430"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXY18064.1"
                     /translation="MLDIRFIRENAEQVQEKAKQKGYNVDIRKLLELDGQRRQIQADI
                     EVVRGERNKLAEAAKGQAPPADAVEKGKHLKQHLTDCEDRLKPLEEEFDKLLRGVPNV
                     FPDDTPLGGEEANAEVKKWGDTADKGFEVKDHLAWGEAGGLIDFERGAKVAGNKFYFT
                     RGTLVELELAVAQLGMELAKKHGFTPMMVPHLVNTRTLEGAGFSAKGEERQIYKVEDE
                     DLNLIATAEIPLTGYHADEILNEKDLPLLYAGLSPAYRVEGGAYGKHNRGLFRTHQFN
                     KLELYVFCKPEDSEDWHQKMVALEEEFCQTLGIPYRIVRIASRDLGAPAYKKFDVEYY
                     SPVDKTYRELMSCSNVTDYQARRLQIRYRHFVPAKETSGGVQPELAVKAGSKVDCVHS
                     LNGTLAAMSRIPIAIIENFQTSDGKVNVPKALQPYMHDQTII"
     gene            4575..4808
                     /locus_tag="VEH48_01435"
     CDS             4575..4808
                     /locus_tag="VEH48_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18065.1"
                     /translation="MMAVAQPVVQYMGGLAVVLVGAGLFASGVLEWCRLEGTKFSGTK
                     AHRWHTIVMPLFVVSCFLLGIGAAMMHSSSFGG"
     gene            4984..7566
                     /gene="secA"
                     /locus_tag="VEH48_01440"
     CDS             4984..7566
                     /gene="secA"
                     /locus_tag="VEH48_01440"
                     /inference="COORDINATES: protein motif:HMM:NF006630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HXY18066.1"
                     /translation="MGDPQAATIKRMRKKVKLINSLADKYQKMSDKELREQTAELKKR
                     LEKGAKLDELLPDAFAVVREAATRTLKQRHFDVQLIGGMVLNEGSVAEMKTGEGKTLV
                     ATLAVYLNALESQGVHVVTVNDYLAQRDAGWMGQIYDFLGMSVGVVIPDESFIYDKTY
                     ENKAHFDERMRHLRPATRQEAYNADITYGTNNEFGFDYLRDNMVRETDQLRQRELHYA
                     IVDEVDSILIDEARTPLIISAPATASGAAYAQFAKVVRQLKETEHYEKDEKRKSVVLT
                     DAGVELVEKILGITNLYATENLRTIYHLEQALRAQTLFHRDKDYVVTKEGEIVIVDDF
                     TGRLLRGRRYNEGLHQAIEAKEGVEVQEESMTLATISFQNLFRLYKKLAGMTGTAMTE
                     SEEFHQVYKMNVIDVPTNQPMVRDDLSDRIYKTELGKMHSIVKDVKQLHEKGQPVLLG
                     TVSIEKNELLSSLLKASNVPHEVLNAKNNEKEAAIIAKAGQKGAVTLATNIAGRGTDI
                     VLGEGITDLGGLFVLGSERHESRRIDNQLRGRAGRQGDPGSTQFYVSCQDDLMRIYGG
                     ERIAAIMDKLKVDDETPIESRMITKSLEGAQKKVEGFNFDQRKNVVQYDDVMNRHRKA
                     IYAMRREILRAQDISPRVKKLMEEEVEWLTKHPESTSENYEAILTETFPFDGDTLDKL
                     FDTAADKFQGELAGAAKKLYQEQEQRFAPETMRKVERDVYLQVLDNLWMQHLENMDHL
                     REGIHWISVGQRDPLVEYRRQGQKIFDEFQATLRHDIVRAIMYAQPLTEQEQVRAVET
                     ELTRAAAGSVDNASQITTAETFEETDFSPLAEEQAREAQLHAQRKKARKAERQRKKKA
                     RRKH"
     gene            7567..8850
                     /locus_tag="VEH48_01445"
     CDS             7567..8850
                     /locus_tag="VEH48_01445"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXY18067.1"
                     /translation="MKHTVSVVQLKNGAHGLFIDIPGATVTSYELNFRAGEYLLKAKN
                     KWEAPHIMEHVILGANEEYPDTQAFQAEFNKNGAFTNAYTSYYYINYIGETADFEWER
                     VLRLQLMALAKPLFLENEFQAEVGNIRDELVSYSNNYFRVLGSEMSKDFGFSIKTDKE
                     RAKLIDNVSRQDVIDHYKRTHFSKNMRFIIAGNLRGRRPALRKILESLELPAGKAGFA
                     LPNEQAKKPPRATFVPNKTVDNIYLTISSYLNDMLSIREDDSLGLSRIMLTDTLYSKI
                     FGQAREKGLVYAVFSGHSQMNKTAEWWLSAQVLPQNAPALCDIILSEIKKVQNGLIDD
                     ASLEAAKQYALGSFQRSMQTVGAVAGAYGRYFFDGYVEDMRSIPERIKAVSKKDMAAV
                     MRRMFSEDISGVGVLGGSDATIGPKLDEQLRPLWR"
     gene            8853..9941
                     /gene="prfB"
                     /locus_tag="VEH48_01450"
     CDS             8853..9941
                     /gene="prfB"
                     /locus_tag="VEH48_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HXY18068.1"
                     /translation="MEELKKRAEKLLHDINSAMTELGADMALQTRDEIRKKMNEPDFW
                     ASPLEAQEESKHESRLTKKYEPWQDIKKSVQDLVELASLNDSSMQTELSGQLDQTEKK
                     YSELKRELRFQGPYDDHDVILSIHAGAGGTDAQDWAQMLLRMYVRWAESQGLKVKTMD
                     QSAGEEAGIKSATIEISNGQFLYGKLAGEHGVHRLVRLSPFNADHLRQTSFAKVEILP
                     VTDKPGELQLDEKDLRIDVFRSGGHGGQSVNTTDSAVRVTHLPTGIVVSMQNERSQLQ
                     NKETALNILRSKLIQLQLEQHAKSLADIKGPNQSAEWGNQIRSYVLHPYKQVKDNRSG
                     YESSDVEKVLDGNLDPLITAWLEQNSVN"
     gene            complement(9938..10423)
                     /locus_tag="VEH48_01455"
     CDS             complement(9938..10423)
                     /locus_tag="VEH48_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18069.1"
                     /translation="MSNFKPVPMTGDQLSPLSSAFTELAVVPTDLTFPVTTVIKNQQT
                     GELVVDSQPDGPYLGPTQLLSTLRRIGSYRAENPELTTDNVHIVAHLGAVATKRLAAI
                     PWEDLPDILSSIQRDLRAVGQGLSIAFISSNLPREKTGKTLTTINSIVNANPRLKGKG
                     R"
     gene            10508..11191
                     /gene="ftsE"
                     /locus_tag="VEH48_01460"
     CDS             10508..11191
                     /gene="ftsE"
                     /locus_tag="VEH48_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HXY18070.1"
                     /translation="MILLDRVSKTYNRRGSALERVSLHIEPKEFVIIVGQSGAGKTTL
                     FRLLTREEKPTSGKIVVGGIDYDKLKDKDIPMLRRKIGVVFQDFKLLPAKTVYENVAF
                     ALEIAGLSNAEIKHTIPKVLDLVGLKGKENNYPLELSGGERQRVAIARAVVRQPKILI
                     ADEPTGNLDPKHAWDVIKVLEKVNKFGTTVLLTTHNQEIVNALRRRVVTIKDGKIVSD
                     RAVGQYKNA"
     gene            11188..12126
                     /locus_tag="VEH48_01465"
     CDS             11188..12126
                     /locus_tag="VEH48_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HXY18071.1"
                     /translation="MKEHRFITFERIIKNGVVNFGRNIWLALAAIAMMSITLTILLFG
                     VVANATFSHTVNDLTSHIDVSVYLKDNVSASQRQQLVQQLNATGNVKNIVYQDKATVL
                     KLYEQANAGNTDLLTAISETSNPLPAKLIISPIDPNNLETIKTVLDKPANANLQSDPT
                     SYSGDRKAAIDKITSATHFFEEAGIVGIAIFVLISMLIIFNTIRMAIFNRRDELVIMR
                     LLGASSRFIRGPFVVETMLYGAVAAAISLIICAALFAIASQTLQASSLGLLDISYSGH
                     FFRAHLWLILSGQIMAGIIIGAASSAFATRRYLRQH"
     gene            12211..13380
                     /locus_tag="VEH48_01470"
     CDS             12211..13380
                     /locus_tag="VEH48_01470"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HXY18072.1"
                     /translation="MLKQKPRTNLRYLLSALFAAAVFGGAAFYNGLARADQFDQQIQA
                     LQNQNAVDAQNAQQFGAQVSNYQDQVNQLEQQIGGLQQSIVTAQQQSDDLQTQINQKQ
                     VELDQQKKVLGEDIKAMYLEGQISTLEILASSKNLSDFVNREVDRSAVQNKLKTSVDQ
                     INSLKLELQQKQLQLQESIKSQQAQQAILQGDENQVSQLLAYNEEQQASLNQQISANN
                     ASIASLRAQQFAANHRLLSGGNVSIVYSGACGGGYPADAISAFGHWGCNYPQDNTLDN
                     WGMYNRECVSYTAWKVYQAYGYMPYWGGSGNANQWPGDADAYSIPRGSTPKVGSVAIG
                     TNPNYFGPVGHAMWVEAVNGDGTIVVSQYNFGEEGMYSEMTINASLIDTFIYFGS"
     gene            13404..14672
                     /locus_tag="VEH48_01475"
     CDS             13404..14672
                     /locus_tag="VEH48_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXY18073.1"
                     /translation="MISQRDNPEPGAAAAAPLSVKRRRPWALKKIFGFVLVLALMFGG
                     GLAVGRGDLHIQGLSVQKSVNSNLPGTLDYSSVNQVYNLLRNDYDGSLNTGKLLDGLK
                     SGLVSAAGDPYTEYFDAADAKTLNDELAGSITGIGAELGTDSDNDIVIVSPLSGYPAD
                     KAGLKPKDVIAAIDGKSTQGISIDTAVSKIRGPAGTKVTLTIVRGGGNPFDVTITRQK
                     ITIPSVTYEVDGDIGYIKISQFTNDTVGLADKTAQSFKAQGVKGVVLDLRGNPGGYLS
                     GAVDVSSLWLKQGQTVVSERRGSTIIDTEYASGDDPLQGLPTVVLIDGGSASAAEITA
                     GALHDNGAATLVGQTSFGKGSVQQVEDLSGGAELKVTIAHWYTPDGKNINQQGITPDV
                     VVSNPTDAQVQAGQDPQKDKAFQIVQNKIQ"
     gene            14906..>15224
                     /gene="pyrG"
                     /locus_tag="VEH48_01480"
     CDS             14906..>15224
                     /gene="pyrG"
                     /locus_tag="VEH48_01480"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF018158.4"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HXY18074.1"
                     /translation="MAAKARAETKYIFVTGGVLSGVGKGITAASIGNILKARGLSVNI
                     QKCDPYLNVDAGTLNPAEHGECFVTFDGAETDMDVGHYERFLDMELTSASSLMSGRIL
                     SEVI"
BASE COUNT         3990 a         4046 c         3987 g         3201 t
ORIGIN      
        1 gttttaagat aacgcagata tcttaaaccc agcttaagat aataaaaggt ccagaaatca
       61 aaattgcaaa tattactaca agcgggggac gatttcaagg agttaactac cttaagcgat
      121 atgtagtgaa aaatacttga cttcaacgcg ggagaggcct atagtggtag ttagtcatcg
      181 tgaggtgacc gtaacaaatc cagcaatgaa tttgttgctt ggtaacggac cttcgggcga
      241 gttatcaaat aaaaaagaga ccctcgtggc ctctttttta ttttctgtat aatgactagg
      301 ctatggtaga agttaccaat aatcagtttg aggagtttat ccaagagggg atagcagcca
      361 tcccgcctct gtaccgtcag cggctgcaaa acgtcgcttt tctgctggaa gacgaaccga
      421 ctccagaaca gcgcaccaaa cttaatcttt atccacacga aacgcttttt ggtctgtacg
      481 agggcgtgcc gctgccgcag cgcggcggta ccactaaaat cctgcccgac aaaatcaccc
      541 tctttaaaaa acctctgcaa aacgccagtc gcgatttaaa cgaccttaag gaagctatcc
      601 gtcacaccgt ctggcacgaa gtcgcccact actatggctt ggaccataaa cgcatccacg
      661 agctggacgg cacatcatcc taattttctg actgagaacg acctggcaaa ccgtggctgt
      721 ggacaaagac ttgaaaagtt gctgagcagg gcatattatg ggtttattaa caacttaagg
      781 aggaaatatg caattctcac ataaatcaag tgtggcctgg atggttcact ttttcgtggg
      841 ggtcgcagag ctgcttctag ccttgcgcgt ggtgctcaga ttctttaacg ggaatccgga
      901 cgctacgttc gtacactggt gctacaccag caccgctgta ctgctagagc cattccgcaa
      961 cgtattcact tctactggag tagtacagcg cggctgggtg gtggactacg tagccttgtt
     1021 cgccatggcg gtctacgcct tgaccggcta cctggctctt ggtctggtca accgctgggt
     1081 caaaagataa tagaaatctc gcataaaaat gaacgcggct tgttgaggca ggccgcgttt
     1141 tgtttattag ctgtacaata gatagttatg gcatatgaga ggtcgccggc ctataagccc
     1201 ggccaaaaag agccgtttaa gattagtcgc agcaagatag atctgtttat ccagtgtccg
     1261 cgctgttatt ggctggacgc ccggttgaag atatcacggc ccagcgggcc gccattcact
     1321 ctgaattcgg ccgtcgacca actgttgaag caagaatttg actctctgcg aaaagctggc
     1381 caacagcatc cgctgcagat tgaatatggc attgacgcca ggccggtcag ccaccccaag
     1441 ttgaatgatt ggcggcacaa cttcacaggg gttcaggcgc tgtataagcc ggctaatttc
     1501 ctagtttttg gcgccattga cgacctgtgg cagaactcca aaggcgaatt tatagtgctg
     1561 gactataagt caaccagcaa agccggcaag attaccgagc tgggacagga aagctggcac
     1621 gaccagtacc gccgccagct gggcgtctac cagtggctct tgcgccaaaa cggcctaaaa
     1681 gtcagcaata ccggctactg gctttactgc aacgccatca aaaacggcaa ggtttttgat
     1741 aataaactgg agtttgagct gacattgatc gccgaaaaaa tagatgacaa atgggttgaa
     1801 ccaacgctgg ccaagatcaa agattgcctg gagggcgaga ttcccaaaga atcacccgac
     1861 tgtgaattct gcgcctacgc ccgctcccgc acccaactaa cgctggactc tattaaagga
     1921 gcacgaggtt agattctatt ctttttcaga aacagggcga catctgcgaa gtctcgtttc
     1981 atggcttcaa aagcaaaact ttcagttgga aggctctttg ggctaaagaa tttatattcg
     2041 gcgacatcgt cagctggctt tggctttgtg ccgcgaggga atttacacac gaagcagaca
     2101 gccaaagttg gataattaac accctggaac gaatacggct ctgtatgcga cccggtgtag
     2161 tgtagctggt ttgacagatc aatacccaat tcttcgtata cttcacgcct ggcgttctct
     2221 tcaaatgttt cattctcctc tacgaatccc cctggcagat cccacaaacc ttttccaggt
     2281 tcaacggctc gtttgaccaa taaatattcg cccttttcgt taatcatgac caccgcagta
     2341 caaggcttag ggttgatata aaaactcaac ccacaagcag ggcacttcaa tagattgtct
     2401 ttgttcccaa attgacttcc gcagcgcgga caaaacttaa agaccttctg tatctccatc
     2461 tttaaatgat aacaggctct agtaggactg ggcttgtttg agtagattta caattgcatc
     2521 agggttgtca ggcccccagt aattcgcaca gctgtccgta acattagtga acaagtaaag
     2581 agtacggccg tcttgcaaga ccattgagcc agagaagtga ttaccgacaa gcgatccgtc
     2641 ttttttagtt atcgaaaagc cagccatccg ttccgcgcag ggaacatctg cgaatgtgtt
     2701 caccaggcga ttaatattga agctggtgtt gatcgacacc tgggcagggg tgttttgggt
     2761 actgtcggcg gcttcgtaat tgtaaagcag attccgcgac aagtcagtca agggcaagta
     2821 tatgcgggct tccggtatat atacccgctc tccaataggg tcgaccactg tttggtcgga
     2881 gtaaagacct ttctcggcgt tttggatata gacctttata tctatggttg tagcgccagt
     2941 tgaactatag aaattggcac tgataatatt ctggcgccat aaaatatcga ttttttggcc
     3001 gaaatcccaa attacggcag tcaatagccc gatcaacatg acaaccagga aaattagtac
     3061 tctgttggtc gggtgttgga actttaattt tttcatatgc tttgctccaa gctaatctta
     3121 agcggaatta tacaccagaa atacaaaatc gactggcaag cgatataata caagctatgc
     3181 tagatattcg gtttatccga gagaatgccg agcaggtgca ggagaaagcc aaacagaaag
     3241 gctacaacgt tgatatccgc aagcttttgg agctggatgg ccagcgccgg caaatccaag
     3301 cggatattga agtagtccgc ggcgagcgta acaagctagc cgaggccgcg aagggtcagg
     3361 cgccgccagc tgacgccgta gaaaaaggca agcatctcaa gcaacatctg accgactgcg
     3421 aagaccgcct caaaccgctg gaagaagaat tcgataagct actgcgcggg gtgcctaacg
     3481 tttttccgga tgacacgccc ttgggcgggg aagaagctaa cgcggaggtc aaaaaatggg
     3541 gcgacaccgc cgacaaagga tttgaagtca aagatcacct ggcttggggc gaggccgggg
     3601 gattaataga ttttgagcgc ggcgccaaag tcgccggcaa caaattctac tttacaaggg
     3661 gaacccttgt agagctggag ctggcggttg cccagctggg catggagctg gccaaaaaac
     3721 acggatttac acccatgatg gtaccgcacc tggtcaatac ccggacgctg gaaggcgccg
     3781 gcttttcggc caagggtgag gaacgacaga tatataaagt tgaagatgaa gacctcaact
     3841 taatcgccac ggccgaaatt ccgctgaccg gttaccatgc cgatgaaatc ttgaacgaga
     3901 aagatctgcc gctgctgtac gccggcctta gcccggctta ccgcgttgag ggtggagctt
     3961 acggcaagca caaccgcgga ctgttccgca cccaccagtt caacaagttg gaactgtatg
     4021 ttttttgtaa gccggaagac agcgaagact ggcaccaaaa gatggtggcg ctagaggaag
     4081 aattctgcca gacgctggga atcccctacc ggatagtccg gattgcctcg cgtgatttag
     4141 gcgccccggc gtacaaaaaa tttgacgtgg aatactacag cccggtagat aagacctacc
     4201 gcgagctgat gagctgctca aacgttaccg attatcaggc gagaagattg caaatccgtt
     4261 atcgtcattt tgttccagct aaagaaacta gtggaggtgt tcaaccagaa cttgcagtaa
     4321 aagcgggaag taaagttgac tgtgtgcaca gtctgaacgg cacgctggcc gccatgagcc
     4381 gcatccccat tgccattatt gagaacttcc agacctccga cggcaaggtc aacgttccca
     4441 aagccttgca gccctacatg cacgaccaaa ccatcattta gctttacaaa agtatacgta
     4501 gatgatataa ttatgatgga ttttactctt aggggtaggg tttccgtcct tcgagagaaa
     4561 ggcaagacat gcacatgatg gctgtagctc agccggtcgt ccagtacatg ggtggcctgg
     4621 ccgtcgtcct agtcggggcc ggtctattcg cctcgggagt tctcgagtgg tgtcgccttg
     4681 agggcacgaa gttctcaggc acgaaggctc accgctggca caccatcgtc atgccacttt
     4741 tcgtcgtcag ctgtttcctg ctgggaattg gcgcggcgat gatgcacagc agcagcttcg
     4801 gtggctgacg tgtaccacat agcctggccg gcgatcgccg gccaggccca gcggcagtaa
     4861 ctctgccgct tttttcatgc gaatttttag agtaaaatat aggttgaccc gtaaagtagt
     4921 tttggcaggt ttttcagttt taatatggtt aagttcttaa atacaaaatc tataaaacgc
     4981 gttttgggtg acccgcaggc cgccaccatt aagcgtatgc gcaaaaaggt caagctcatc
     5041 aatagcctgg ccgataaata ccaaaaaatg tccgataagg aactgcgcga acaaacggcg
     5101 gagcttaaga agcggctgga aaagggcgct aagctggacg agctgttgcc cgacgccttt
     5161 gccgtggtgc gcgaggcggc cacccgcacg ctgaagcagc ggcactttga cgtgcagctg
     5221 atcggcggca tggtgctgaa cgaaggctcg gtggccgaga tgaaaaccgg cgaaggcaaa
     5281 acgctcgtag cgaccctggc ggtatatctc aacgcgctgg aaagccaggg cgtgcacgta
     5341 gtgaccgtca acgactacct ggcgcagcgc gacgccggct ggatgggcca gatttatgac
     5401 tttttgggca tgagcgtcgg cgtggtcatc cccgatgaat cattcattta tgacaaaact
     5461 tacgagaaca aggcccactt tgacgagcgc atgcgccacc tgcgcccggc cacccgccag
     5521 gaagcctaca acgccgacat cacctacggt accaacaacg aattcggctt tgactacctg
     5581 cgcgacaaca tggtgcgcga gaccgaccag ctgcgccagc gcgaactgca ctatgccatc
     5641 gtggacgaag tggacagcat cttgattgac gaagcccgca ctcccctgat catcagcgcg
     5701 ccggccaccg ccagcggcgc ggcttatgcc cagttcgcca aagttgtccg ccagctcaaa
     5761 gaaaccgaac attatgaaaa ggacgaaaag cgcaaaagcg tggttctgac tgacgccggc
     5821 gtggaactgg tagaaaaaat cctgggcatc actaatttat acgccaccga aaatctacgc
     5881 actatttacc acctggaaca ggccctgcgg gcgcaaacgc tgttccaccg cgacaaagat
     5941 tacgtagtta ccaaagaggg cgagatcgtc atcgttgacg actttaccgg ccgcctgctg
     6001 cgcggccggc gctacaacga aggtctacac caggctatcg aagccaaaga gggcgtggaa
     6061 gtccaggaag aatccatgac cctggccact atctctttcc aaaacctctt ccgcctgtat
     6121 aaaaaattgg ccggcatgac cggcacggcc atgaccgaaa gcgaagagtt ccaccaggtt
     6181 tacaagatga acgtcataga tgtgccgacc aaccagccga tggtccgcga cgacctgtca
     6241 gacaggattt acaaaaccga gctgggcaag atgcattcca tcgtcaaaga tgtcaagcag
     6301 ctgcacgaaa aaggccagcc ggtactgctg ggcacggtca gcattgagaa gaacgaactg
     6361 ctgagctctt tgctgaaggc ctccaatgtg ccgcacgaag tgttgaacgc caagaataac
     6421 gagaaagaag cggcgattat cgccaaagcc ggccaaaaag gcgctgtgac cttggcgacc
     6481 aacatcgccg gccgcggtac cgacatcgtc ttgggcgagg gcataaccga cttgggcggc
     6541 ctgttcgtgc tgggcagcga gcgccacgaa tcgcggcgca ttgacaacca gcttcgcggc
     6601 cgggccggtc gtcagggcga tcccggcagc acgcagtttt acgtatcttg ccaagacgac
     6661 ctgatgcgca tttatggcgg cgaacgcatc gccgccatca tggacaagct taaagtggac
     6721 gacgagacac cgatagaatc gcgcatgatt accaaaagcc tggaaggcgc ccagaaaaaa
     6781 gttgagggct tcaactttga ccagcgcaaa aacgtcgtgc agtacgacga cgtcatgaac
     6841 cgccaccgca aagccattta cgccatgcgc cgcgagattc tgcgcgccca ggacatcagc
     6901 ccgcgcgtta aaaaactcat ggaagaggaa gtggaatggc tgaccaagca ccccgaatca
     6961 accagcgaaa attacgaggc catccttacc gagactttcc cgtttgacgg cgatactctg
     7021 gacaaactgt tcgataccgc cgccgacaaa ttccagggcg agctggctgg ggccgccaag
     7081 aaactctacc aagagcagga acagcgtttt gcgccggaaa ccatgcgcaa agtggagcgc
     7141 gacgtttacc tgcaagtgct ggataacttg tggatgcagc acctagagaa tatggaccac
     7201 ctgcgcgagg gcatccattg gataagcgtc ggccagcgcg atccgctggt ggaataccgc
     7261 cgccaaggcc agaaaatatt tgatgagttc caagccacgc tgcgccacga tatcgtccgg
     7321 gctatcatgt acgcccagcc gctgaccgag caggaacagg tccgggcggt agagaccgaa
     7381 ctaactcgtg ccgccgccgg ctcggtagac aacgccagcc aaattaccac cgccgaaact
     7441 tttgaagaaa ccgactttag cccgctggcc gaggaacagg ctcgcgaagc ccagctgcac
     7501 gcccagcgca aaaaagcccg taaggccgaa cgccaacgca agaaaaaagc ccggaggaag
     7561 cactagatga aacataccgt tagtgtggtt caactgaaaa atggagccca cgggcttttt
     7621 attgacattc ccggcgccac tgttaccagc tacgaattaa acttccgcgc cggcgaatac
     7681 ctgctgaagg ccaagaacaa atgggaagcg ccgcacatta tggagcacgt cattttgggc
     7741 gccaacgaag agtatcctga cacccaggct ttccaagccg aattcaataa aaacggcgct
     7801 tttaccaacg cctataccag ctattattac atcaattata tcggcgaaac cgccgatttt
     7861 gagtgggaac gggtgctgcg cctgcagctg atggccctgg ccaagccgct gtttctggaa
     7921 aatgaattcc aagccgaggt tggcaatatc cgtgatgagc tggtcagcta ctccaacaac
     7981 tatttccggg tgctgggcag tgagatgagc aaggattttg gctttagcat caagaccgac
     8041 aaagaacgtg ccaagctgat tgacaatgtc agccgccaag atgtcataga ccactacaag
     8101 cgtacgcatt tcagcaaaaa tatgcgtttt attatcgccg gcaatctgcg cggccgccgg
     8161 ccggctctgc gaaaaattct ggaatcgctg gaactgccgg caggcaaagc cggctttgcg
     8221 ctgcccaacg agcaagctaa aaaacccccg cgggcgactt ttgtgcccaa caaaacagta
     8281 gacaatattt atctgaccat cagcagctac ttgaatgata tgctcagcat ccgcgaggac
     8341 gattcgctag gcttgtcgcg catcatgctt accgacacgc tgtattccaa aattttcggc
     8401 caggcgcgcg aaaaagggct ggtttacgcc gtattttccg gccacagcca gatgaataaa
     8461 accgctgaat ggtggctttc cgcccaggta ttgccgcaaa acgccccggc actatgcgac
     8521 atcatcctat cagaaatcaa aaaggtccaa aacggcctga tcgacgacgc cagcctggag
     8581 gccgccaaac agtacgcgct gggttcattc cagcgctcaa tgcagacagt cggcgccgtg
     8641 gccggcgctt acggccgcta cttttttgac ggctacgtag aagatatgcg gtccatcccc
     8701 gagcgtataa aggccgtcag caaaaaggac atggcggcgg ttatgcgccg gatgttcagc
     8761 gaagatatca gcggcgtggg cgtgcttggc ggcagcgacg cgacaatcgg cccgaagctc
     8821 gatgagcagt tacgccctct ttggaggtga agatggaaga gctaaaaaag cgagctgaaa
     8881 aactgctcca tgacatcaat tctgctatga cggagcttgg ggctgatatg gctcttcaaa
     8941 ccagggatga aattcgtaaa aaaatgaatg aaccagattt ttgggctagc ccgctggagg
     9001 cccaggagga aagtaaacat gaaagtcgct taacgaaaaa atatgagccg tggcaagata
     9061 taaaaaaatc agttcaagac cttgttgagc tggcaagttt aaatgattct tcaatgcaaa
     9121 ctgaactttc aggccagctt gatcaaaccg aaaaaaagta ctctgaactt aaaagagaac
     9181 tacggtttca aggaccatac gacgaccacg acgtcatcct gagtatccac gccggcgccg
     9241 gcggcaccga cgcccaggac tgggcgcaga tgctgctgcg aatgtacgtg cgctgggccg
     9301 aaagccaagg actaaaggtc aaaaccatgg accagagcgc cggcgaagaa gccggcatca
     9361 aaagtgccac cattgagatt tccaacggcc aattcctgta cggtaagcta gccggcgagc
     9421 acggcgtgca ccggctggtt cgtttaagtc cgttcaatgc cgaccatctg cgccaaacca
     9481 gctttgccaa ggtggaaata ttgccggtga ctgataagcc cggtgagctt caactggacg
     9541 agaaagattt gcggattgac gtttttcgct ccggcgggca cggcggccag tcggtcaaca
     9601 ccaccgactc ggccgttagg gtcacgcact tgccgaccgg cattgttgtc agcatgcaga
     9661 acgagcgcag ccagctgcaa aacaaggaaa ccgcgctgaa tattttgcgc tcaaaactta
     9721 tacagctgca gctggaacag cacgccaaaa gcctggccga tatcaaaggc cccaaccaat
     9781 cggcggagtg ggggaaccag atccgcagct acgtcctgca cccgtataag caggtcaagg
     9841 acaaccgcag cggctacgaa tcctccgatg ttgaaaaagt actggacggc aacctggacc
     9901 cgctcatcac cgcctggctc gaacagaact cagtcaatta acgaccttta cctttaagac
     9961 gagggttggc gttcacgata gaattgatcg tagtgagcgt ttttccagtt ttttctcttg
    10021 gcaaattgct gctaataaaa gctatgctaa gtccctggcc gactgccctt aagtctcttt
    10081 gtatcgatga caatatgtcg gggagatctt cccacggtat ggcggcaagt ctttttgtag
    10141 caactgctcc aagatgagcc acgatatgta cattgtctgt ggtaagttca ggattctcag
    10201 ctctgtagct gccaattctt cgaagcgtac ttaagagctg cgtaggaccc agatagggcc
    10261 catcaggttg gctatcaaca accaactcgc cagtttgctg gttttttatg actgtagtga
    10321 ctgggaaagt caggtccgtt gggactacag cgagttcggt aaacgcactt gacaaaggac
    10381 tgagttggtc gccggtcatg ggcaccggtt taaaattgga catcgcatcg ttattatagc
    10441 ataaaatgtg tatagattgg gtcattgaac tcaatctgat aatgtttgtc tggtatactt
    10501 attaacgatg attttgctcg acagggtcag taaaacctac aaccgcagag ggtcggcgct
    10561 ggagcgcgtc tcgctgcaca ttgagcccaa ggaattcgtg attattgtcg gccagagcgg
    10621 cgctggtaag accacgctgt tccgcttact gacgcgcgaa gaaaaaccga ccagtggcaa
    10681 aatcgtggtc ggcggcatag actacgacaa gctcaaagat aaggacatcc cgatgctgcg
    10741 gcgcaaaatt ggtgtggtct tccaggattt taagctcctg ccggcaaaaa cagtttatga
    10801 gaacgtggct ttcgcgctgg aaattgccgg cctatccaac gccgaaatca aacacaccat
    10861 tccaaaagtg ttggatctgg tcggcctgaa aggcaaggag aacaattatc cgctggagct
    10921 gtccggcggt gagcgccagc gcgtggctat agcccgggcc gtggtgcgcc agcccaaaat
    10981 cctgatagcc gacgagccca ccggtaacct ggaccccaaa cacgcctggg acgttattaa
    11041 agttttagaa aaagttaata agtttggcac tacggtactg ctgaccaccc acaaccagga
    11101 aatcgttaac gccctgcgcc gtcgggtggt tactatcaag gacggtaaaa tcgtatccga
    11161 ccgggcggta gggcagtata aaaacgcatg aaagagcacc ggtttataac ttttgagcgg
    11221 ataatcaaaa acggcgtggt taattttggc cgcaacatct ggctggcgct ggcggcaatc
    11281 gccatgatga gtattaccct gaccattttg ttgttcggtg tggtggccaa cgccaccttc
    11341 agtcatacag ttaacgatct gaccagtcac atcgacgttt cggtttatct gaaagacaac
    11401 gtcagcgcca gccagcgcca gcagctggtc cagcagctca atgccaccgg caacgtcaaa
    11461 aatatcgtct accaagacaa ggccactgtg cttaagcttt atgagcaggc caacgccggc
    11521 aacaccgacc tgctgaccgc catatccgag acttccaacc ccctgccggc taagctgata
    11581 atcagcccga ttgaccccaa taatctggag accatcaaga cagtgctgga caagccggcc
    11641 aacgcaaatc tgcagtctga ccccaccagc tacagcggcg accgtaaggc cgcgattgat
    11701 aaaatcactt ccgccaccca cttttttgag gaagccggta tcgtcggcat cgccatattc
    11761 gtgctgattt ccatgctgat aatcttcaac accatccgta tggccatctt caaccgccgc
    11821 gacgaactgg tcatcatgcg cctgctgggc gccagcagcc gtttcatccg cgggccgttc
    11881 gtggtggaga ccatgctcta cggcgcggtg gccgcggcaa tctcactgat aatctgcgcc
    11941 gcgctgttcg ccattgccag ccagacgctg caagccagca gcctgggcct tctggacatt
    12001 agttattccg gccatttttt ccgcgctcac ctctggttga tcctgtcggg gcagattatg
    12061 gctggtataa taattggagc cgcttcttcg gcctttgcca cgcggcgcta tctgcggcag
    12121 cactagaggc tgtcccctag actaaataaa gattttttaa aggtgggcag gttgacttaa
    12181 ccccgtggag ggattatatt gaaagcgttc atgctaaaac aaaaacctag aactaatctg
    12241 agatatttat tatcggcgct ttttgcggcg gccgttttcg gcggcgcggc gttttataac
    12301 ggtttggcgc gcgccgatca attcgaccag caaatacaag ccctgcaaaa tcaaaacgcc
    12361 gttgatgccc aaaacgcaca gcagttcggc gcccaggtca gcaattacca ggaccaggtc
    12421 aaccagctgg aacaacagat aggcggcctg cagcaatcaa tcgtgaccgc ccagcagcag
    12481 agcgacgacc tgcagacaca aattaatcaa aaacaagttg agcttgacca gcagaaaaag
    12541 gtcttgggcg aagacatcaa agccatgtat ctggaaggcc agatttccac cctggagatt
    12601 ttggccagca gcaagaacct gagtgatttt gtcaacaggg aagtggaccg cagtgccgtc
    12661 cagaataagc tcaagacctc ggttgatcag attaatagtt tgaaactgga gctgcagcaa
    12721 aagcagctgc agctgcagga atcaatcaag agccagcagg cccagcaggc cattctgcag
    12781 ggcgacgaga accaggtgag ccagctgctg gcctacaacg aagaacagca ggccagctta
    12841 aaccagcaga tcagcgccaa taacgcctcc atcgcttcac tacgggccca acagttcgcc
    12901 gccaaccacc gtctgctgag cggcggcaac gtgtcaatcg tctacagcgg cgcctgcggc
    12961 ggtggttatc cggcggacgc cattagcgct tttggtcact ggggctgcaa ctatccgcag
    13021 gataacaccc tggacaattg gggtatgtat aatcgcgaat gcgtcagcta caccgcctgg
    13081 aaggtttacc aggcatacgg ttatatgccg tattggggag gttcgggcaa cgccaaccag
    13141 tggcccggcg acgccgacgc ttatagcatc ccgcggggtt caacccccaa agtcggttca
    13201 gtggccattg gcaccaatcc gaattatttc ggccccgtcg gccacgccat gtgggtagag
    13261 gccgtcaatg gtgacggcac catcgtcgtc agccaataca acttcggcga agaaggaatg
    13321 tacagcgaga tgaccatcaa tgccagcctg attgacacct tcatatactt cggcagttaa
    13381 agcttacgct ataataattc ctagtgataa gtcagcgaga taatccggag cccggcgccg
    13441 cggccgccgc gcctttgtcc gtcaaacgcc gccggccctg ggcgttgaaa aagattttcg
    13501 gctttgtgtt ggtcttggcg ctgatgtttg gcggcgggct ggcggtcggc cgcggcgacc
    13561 tgcacatcca ggggctgagt gtccagaaga gcgtcaactc caatctgccg ggcacgctgg
    13621 attattcatc ggttaaccag gtctataact tgctgagaaa cgactacgac ggcagtctga
    13681 acaccggcaa actgctggac gggctgaaaa gcggcctggt cagcgccgcc ggcgatcctt
    13741 acaccgaata ttttgatgcc gccgacgcca aaacgctcaa tgacgagctg gccggcagca
    13801 tcaccggcat cggtgccgag ctgggcaccg acagcgataa cgacatagtg attgtctcgc
    13861 cgcttagcgg ctacccggca gacaaggccg gcctaaagcc caaagatgtt atcgccgcca
    13921 tagacggtaa atccacccaa ggcattagca tagacaccgc ggtcagcaaa atccgcggtc
    13981 ccgcaggcac caaagtgaca ctgaccatcg tccgcggcgg cggcaacccc tttgacgtta
    14041 ctatcacccg ccagaaaatc accatcccca gcgtcaccta cgaagtagac ggcgacatcg
    14101 gctacatcaa aatcagccag tttaccaacg ataccgtcgg cctggccgac aaaaccgccc
    14161 agtccttcaa agcccaaggc gtaaaaggtg tggtgctgga cctgcgcggc aaccccggcg
    14221 gctacctctc cggcgccgtg gatgtctcca gcctatggct caaacagggg cagacggtgg
    14281 tgtccgagcg ccgcggcagc acaatcattg acaccgaata cgccagcggc gacgaccctc
    14341 tgcagggcct gcccacagta gtgctaatag atggcggcag cgccagcgcg gcggaaatta
    14401 ccgccggcgc actgcacgac aatggcgccg ccactttggt cggccagaca agcttcggca
    14461 agggcagcgt ccagcaggtg gaagacctca gtggcggcgc cgaactcaaa gtcaccatcg
    14521 cccactggta cacccccgac ggcaaaaaca tcaaccagca gggcatcacg ccggacgtgg
    14581 ttgtcagcaa ccccacggac gctcaggtcc aggctggcca ggacccgcaa aaagacaaag
    14641 ccttccagat cgtgcaaaac aaaatccagt aataattcct aattgttcta attgctctaa
    14701 tttctaagaa aatcccaatt accaaatccc aagtttttag tactttgtgc ttggttaatt
    14761 atttaggtta attagaaatt aggtcaatta ggtaaatttt ttaaattact ttacttgtca
    14821 agtatttttc tactgcagcg ctcgccatag ttttcatgtt aatataagcc gtaattaagg
    14881 cttgagggag cgggcaggtt taaccatggc ggctaaagcc agggcagaga ctaaatatat
    14941 attcgtaacc ggcggtgtac tatccggcgt cggcaaaggt atcaccgccg cttctatcgg
    15001 caatatctta aaggcccgcg gcctatcggt taatatccaa aagtgcgatc cctatcttaa
    15061 cgttgacgcc ggtacgctca atccggccga acacggcgag tgctttgtga cgtttgacgg
    15121 cgccgagacc gatatggacg tcggacacta tgagcgtttt ttggatatgg agctgaccag
    15181 cgccagttca ctgatgagcg gccgaattct aagcgaggtt atca
//
LOCUS       DASPTF010000045         2685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1222104, whole genome shotgun sequence.
ACCESSION   DASPTF010000045 DASPTF010000000
VERSION     DASPTF010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222104"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..564
                     /gene="greA"
                     /locus_tag="VEH48_01485"
     CDS             121..564
                     /gene="greA"
                     /locus_tag="VEH48_01485"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HXY18075.1"
                     /translation="MRKLFHLTQSGIDELKVELDGLLSQRGAVAERIRQARELGDLSE
                     NAEYQTAREEQDRLETRISEVEHVLNNIQPIKKPKSNGEVKLGSTVTIKDDKILHFQV
                     VGTMEADPLNGKVSDESPIGKMLMGKKVGDKVALNGITYKITHIG"
     gene            595..2121
                     /gene="lysS"
                     /locus_tag="VEH48_01490"
     CDS             595..2121
                     /gene="lysS"
                     /locus_tag="VEH48_01490"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HXY18076.1"
                     /translation="MQWLNKVADEVMAAHPDGEILIESGVSPSGTYHVGTLREVLTCD
                     AVMLELRRRGRSARHIHYVDDLDVFRKVPNNVPPTHEKFLGKPLCDVPAPDGSDQSYA
                     DYFLNDFLAAAKKLHMDMEVIRSHEKYRAGAMTEVIEIALTNVDKIRTAIETISGRQL
                     EKDWAPIQVLEDGYLKNRRFVSIDKAAKTVVYLDAEGQQQTTSYAKGEVKLNWRVDWP
                     ARWWQLGVDVEPFGRDHATKGGSYDTGAEIVKQVFGGTAPYPVPYNFINLTGETKKMS
                     KSTGSIIAISDFTKVMPAEVVRYFTLRYAPEKLLFFDPSEGVVKLIDDYAALLAGQGS
                     DDDKQTIDICSVGTDTTVSSVPFSHLVASYQAALKDPVKTLEIIKRTEHGDTVTAQEE
                     IIKKELGFIEQWLAKWAPEDVKFELAEKIDSGRFTDEQKQLLAQLADKIANAPADADG
                     EWFHKAIYELKEAGSLSPEQVFKPLYQALIGKDSGPRAGWFLSILPRDWLIKRLRLES
                     "
     gene            2165..>2685
                     /locus_tag="VEH48_01495"
     CDS             2165..>2685
                     /locus_tag="VEH48_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HXY18077.1"
                     /translation="MLQSARGGVPSSRVTPGILGLAAIRAAPQLLGWVLCRRLPEGVV
                     KLKIVETEAYHQDDPASHSFRGQTARTAPMFRVGGHLYVYFTYGRHYALNIVTGPAGR
                     GEAVLLRAGEPLASIDIMQRNRKITDVRKLAAGPGRLAQALGITNTSLSGTELLNMLS
                     NSKISKPAPLQNL"
BASE COUNT          693 a          745 c          745 g          502 t
ORIGIN      
        1 gaccagctag acaaactact ggccaacctc accgcggatt gatataattc atcaaatcct
       61 caggtggtta aagctaaaga aagcgaataa gagatagcta acgtgaaagc gaacttagcg
      121 atgagaaaac tgttccattt aacccagtct ggtatagatg agctcaaggt agagctagat
      181 gggctgttgt cccagcgcgg ggcggtggcc gagcgcatcc gccaagcacg cgagctgggc
      241 gatttatcgg aaaatgccga ataccagacc gcccgcgaag agcaggaccg gctggaaacg
      301 cgtatatcag aggtggagca cgtattaaat aatatccaac ccatcaaaaa gccaaaatcc
      361 aacggcgagg tcaaactggg ctccacagtc accatcaaag atgataaaat cctgcacttc
      421 caagtggtcg gcaccatgga agccgacccg ctgaacggta aggtcagcga cgaatcgcct
      481 atcggcaaaa tgctcatggg caaaaaagtc ggtgacaaag tcgcgctcaa cggcatcacc
      541 tacaaaatca cccacatcgg ctaaaagcta aaagctttaa gctataattc ttgtatgcag
      601 tggcttaata aagtcgccga tgaggtgatg gccgcccacc cggacggcga gatattgatt
      661 gaatcgggcg tttcgccctc cggtacttac cacgtaggta ctttgcgcga ggtcctgact
      721 tgcgacgccg ttatgctgga actgaggcgc cgcggccgga gtgcccgcca catccactac
      781 gtggatgatc tggacgtttt ccgcaaagtg ccgaacaacg tgccgccgac tcacgagaaa
      841 ttcctcggta aaccgctgtg cgacgtgcca gcacccgacg gctccgacca aagctacgcc
      901 gactattttt tgaacgattt tctggcggcc gctaaaaagc tgcatatgga tatggaagtt
      961 atccgcagcc acgaaaaata ccgcgccggc gccatgacgg aagttataga gatcgccctg
     1021 accaacgttg acaaaattcg cacggccata gagacgatct ccggccgcca gctggaaaaa
     1081 gactgggcgc cgatccaggt gctggaagac ggctatctga aaaaccgccg cttcgtatca
     1141 atagataaag ccgccaaaac ggttgtttat ctggatgccg agggccagca gcagacgacc
     1201 agctacgcca agggtgaagt gaaactaaac tggcgcgtgg actggccggc gcgctggtgg
     1261 cagctgggcg tggatgtgga gccgttcggc cgcgaccacg ccaccaaagg cggcagctac
     1321 gataccggcg ccgaaatcgt caagcaggtc tttggcggca cggccccgta cccggtgccc
     1381 tataacttta taaatctcac cggcgagact aaaaaaatga gcaaaagcac cggcagcatc
     1441 atagctatct cagactttac caaagttatg ccggccgagg tcgtacgcta tttcacactg
     1501 cgctacgcgc cggaaaaact gctgtttttc gaccccagcg agggcgtggt caaactgata
     1561 gacgattacg cggcgctgct ggccgggcag gggtcggacg acgacaaaca gaccatagac
     1621 atttgcagcg tcggtacgga tacgacagtc agctcagtac cgttctcgca tctggtggcc
     1681 agctatcagg cggccctaaa agacccggtg aaaaccttgg aaatcatcaa gcgcaccgag
     1741 cacggcgata cggttaccgc ccaggaagaa attatcaaaa aagagctggg gtttattgaa
     1801 caatggttgg ccaaatgggc gccggaagac gttaaattcg agctggccga aaaaatcgat
     1861 agtggtaggt ttacagatga gcagaagcag ctgctggccc aattggctga taaaatcgcc
     1921 aatgctccgg ctgacgccga cggcgagtgg ttccacaagg ccatttacga gcttaaggag
     1981 gccggcagcc tgtcgcccga acaagttttt aaaccactct accaagcgtt aatcggcaaa
     2041 gactccggcc cgcgcgccgg ctggttccta tcaatactgc cgcgtgactg gttaatcaag
     2101 cgcctccgtc ttgaaagcta agcgccaaaa tcctggggtc ctgccgctcg aggcacctcc
     2161 caccgtgctc caaagtgcgc gcggcggggt gccctcctcc cgcgtcaccc ccggtatttt
     2221 gggcttagcc gctatcagag cggcgccgca gctgctgggg tgggttttgt gccggcggct
     2281 gccggaaggt gtagttaagc ttaaaatcgt ggaaaccgag gcttaccatc aggatgaccc
     2341 ggccagccac agcttccgcg gccagacggc gcgcacggcg ccgatgttca gagtcggcgg
     2401 acatctttac gtctatttca cttacggcag gcactacgcg ctcaacatcg tgaccgggcc
     2461 ggcgggcagg ggagaggcgg ttttgctgcg ggccggcgaa ccactggcga gcatagatat
     2521 tatgcaacgg aaccgcaaaa taacagatgt tagaaagtta gccgccggtc cgggccgttt
     2581 ggcccaggcc cttggcatca ccaacaccag cctctctggc acggaattac ttaacatgtt
     2641 aagtaattct aaaatcagca agcctgcccc gttgcaaaac ctgag
//
LOCUS       DASPTF010000046         3578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_944941, whole genome shotgun sequence.
ACCESSION   DASPTF010000046 DASPTF010000000
VERSION     DASPTF010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944941"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VEH48_01500"
     CDS             <1..201
                     /locus_tag="VEH48_01500"
                     /inference="COORDINATES: protein motif:HMM:NF018531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HXY18078.1"
                     /translation="RLAIKKGGSTDRNYVNAKGKKGSYIQFASVFRREGQPCPRCGTP
                     IEKIRVAGRGTHICPHEQKAPK"
     gene            198..1112
                     /gene="holA"
                     /locus_tag="VEH48_01505"
     CDS             198..1112
                     /gene="holA"
                     /locus_tag="VEH48_01505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HXY18079.1"
                     /translation="MIISLTGNNSYLLKRRLDELVAAFVAEHGELALERIDAAEADVQ
                     AIIDAVQGLPFLASRKMVVVRDLSANKQAAEQVEQIISSNGKTTDLVFYEPLTDRRTV
                     FYKTLKQKTQFEEYDELPPRELPKWLVGEAKKSGGELSLADANYLVERVGPNQAMLAS
                     ELEKLIIYSPKIVRETIDLLTDPAPQSKIFDLLDAAFGGNKKRALKLYEEQRAQRVEP
                     QAIMAMLAWQLDLLALAKHAKSRTPSEVARDAGISPYPFTKAKNLAAKISKDELNKMV
                     NDALEIDLRAKTTALNLDEALKTYITTL"
     gene            complement(1159..1419)
                     /locus_tag="VEH48_01510"
     CDS             complement(1159..1419)
                     /locus_tag="VEH48_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18080.1"
                     /translation="MALLYGCGVFDHPILSTFAFTGIAAGGVLSFVTLPTNMTNAVFW
                     IGAVLGLISALAGMMGIALGLDHLLSKRRIARSNLRFSKQVA"
     gene            complement(1635..1979)
                     /gene="rpsT"
                     /locus_tag="VEH48_01515"
     CDS             complement(1635..1979)
                     /gene="rpsT"
                     /locus_tag="VEH48_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXY18081.1"
                     /translation="MPIIKSAKKRVKVAAKANARNERAKRAMRASLKAFAKALEGQKT
                     DQIAKTQQAAVKSIDQAAKKAVIHKNKAARYKARLAREAKAAGAKPSKSESKKAVTPV
                     KKPAAKKPSAKK"
     gene            2221..>3578
                     /locus_tag="VEH48_01520"
     CDS             2221..>3578
                     /locus_tag="VEH48_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18082.1"
                     /translation="MDAQAKQQIVERVKQANNVLVTVSNNPSVDQLAACIGLTLLLNR
                     MDKHGTAVFSGAVPSTIEFLQPEKTIETNTDSLRDFIISLDKNKADKLRYKVEDQVVR
                     IFITPYKTSLSEKDLVFSQGDINVDVVIALGVHDRNQIDAAITAHGRILHDATVISLN
                     AGQGKAPELGQINWQDPAASSLSEMLVSISEAFGPGLIDNQIATAFLTGIVAETQRFS
                     NPKTTPKTMTMAAQLMAAGANQQLIVSKLEPPSPPTGSSGPRMPAPPKPSSPKKPQDK
                     GTLNVSHDKNKPDSGEVEIAPGEIHIDDRGNLKTTEELKAELERLKKMRGEMTMMPEP
                     TPAPLPAEPAPAPPPPPTPAPPPAEPIPPAPPIPSDGGMTTPIVVSPEPKPHELLDPM
                     DHRPTFNAPFTADTQSEWIDPYSTVTIDPLSGNTGGPPGGDGFAGNERVVQPPPEPPK
                     TLL"
BASE COUNT          916 a          991 c          954 g          717 t
ORIGIN      
        1 cgcctagcca tcaaaaaagg cggcagcacc gaccgcaatt atgtgaacgc caagggtaaa
       61 aaagggagct atatccagtt cgccagtgtt ttccggcgcg aaggccagcc ttgcccgcgc
      121 tgcggcacac ccatagaaaa aatccgcgta gccggcagag gaactcatat ttgtccgcac
      181 gaacagaaag cacctaaatg ataatcagtt tgaccggcaa taattcgtat ttattgaagc
      241 gcaggctcga tgagttggtg gcggcgtttg tggcggagca cggcgagctg gcgctggagc
      301 ggatcgatgc tgccgaagcc gatgtccaag ccataattga cgctgtgcaa ggcctgccgt
      361 ttctggcatc acgcaagatg gtcgtggtgc gcgacctgtc ggctaacaag caagcagccg
      421 agcaggttga gcaaattatt agctcaaacg gaaagaccac tgaccttgta ttttatgagc
      481 ctttaacaga taggcgaacg gttttttata aaaccctgaa acaaaaaacg cagtttgaag
      541 aatatgacga gttgccgccg cgcgagctgc ccaaatggct ggtcggtgag gccaaaaagt
      601 ccggcggcga gcttagcctg gccgacgcaa attatttggt ggagcgggtc ggccccaacc
      661 aagcaatgct ggccagcgag ctggaaaaat tgataattta cagcccaaaa atcgtgcgtg
      721 aaaccattga tttgctaacc gacccggcgc cgcagagcaa gatttttgat cttttagacg
      781 cggcttttgg cggcaataaa aaacgcgcgc tgaaacttta tgaagaacag cgcgcccagc
      841 gcgtggaacc gcaggccatc atggccatgc tggcctggca gctggacttg ctggcgttgg
      901 ccaaacacgc caaaagccgc acgccatcag aggtcgcccg cgatgccggc atcagcccgt
      961 accccttcac caaagccaaa aacctggcgg ctaaaatcag caaagacgag cttaataaga
     1021 tggtcaacga tgccttagaa attgacctgc gcgctaaaac caccgcgctg aacctggacg
     1081 aagccttgaa aacctatata acaaccctat aactatgaag acggggtccg ctgcttgtgg
     1141 cagcgaaccc cgtgtccttc acgcgacctg cttcgagaac cgaaggttcg agcgcgcgat
     1201 gcgtcgcttg ctcagcaggt ggtcgagccc gagtgcgatc cccatcattc ccgctaacgc
     1261 ggagatcagc cccagaacgg cgccgatcca aaagaccgca ttggtcatgt ttgtggggag
     1321 ggtgacgaag ctgagcacgc cgccggcggc gatgccggtg aaggcaaacg tgctgagtat
     1381 ggggtggtcg aagacgccac atccgtagag cagcgccatg aaccacctcc acaggttcca
     1441 tgcgcccctt acgagcgcaa caacgagagc gagaaccccg gacaacaagc tcctaaacac
     1501 ttggtcccct ttcgggtggc tgacggcaaa ggacattctg gaggaggtac tgttcgctgg
     1561 cgaacacctc tgaaattcca gaacttgaaa attataccat aagtttaatt aaaatgcaac
     1621 caactagagt ggttttactt cttggcggaa ggttttttag ccgctggttt tttcacaggg
     1681 gtgacggctt tcttggactc agacttagac ggtttggcgc cggcggcttt ggcttcgcgg
     1741 gctagccgcg ctttgtagcg ggcggccttg ttcttatgaa taacagcctt tttggcggct
     1801 tggtcaatag acttgaccgc ggcttgctga gtcttagcaa tctgatctgt tttttggcct
     1861 tccagagctt tggcgaaagc cttcagagaa gcgcgcatag cacgcttggc gcgctcattt
     1921 cgggcattag ccttagcggc caccttcacc cgttttttag cagactttat aatcggcatc
     1981 gccaagctac tatacagatg acgacccctt ttgtcaaaga tgcgttttta cgcttgcgct
     2041 tagtatcggc ttatggtaat ctgaacccca gaagtcacct cacaaaaact tcacaaaaaa
     2101 taaaaatgaa cgaatgaaca aatgttcaat gttcaatgaa tggtcaatga agcaatgtag
     2161 caatgcattg tccattgtcc atttgtcgca tttgtccatt gcctccggag gaggcgtgta
     2221 atggacgctc aagccaaaca acagatagta gagcgcgtca aacaggccaa caatgtgttg
     2281 gttactgtca gtaataaccc gtcggtggac cagctggcgg cctgtatcgg gttgacgcta
     2341 ctgcttaacc gtatggataa gcacggcacg gcggtgtttt ctggcgcggt gcccagcacc
     2401 attgagtttt tgcagccgga aaagaccatc gagaccaata ccgacagtct gcgcgacttt
     2461 ataatcagcc tggataagaa caaggccgac aaactgcgct acaaggtaga agaccaggtg
     2521 gtgcgtatct ttattacacc ttataaaact tctttaagtg aaaaagacct ggttttcagc
     2581 caaggcgaca tcaacgttga cgtggtaatc gccctgggcg tgcatgaccg caaccagata
     2641 gacgccgcca tcactgctca cggccggatt ttgcacgacg ccacggtaat cagcctgaac
     2701 gccgggcagg gcaaagcgcc cgagctgggc cagatcaact ggcaggaccc ggctgccagc
     2761 agcttaagcg agatgctggt gagtatcagc gaggccttcg gtccgggttt gatagacaac
     2821 cagatcgcca ccgccttttt gacaggcatc gtggccgaga cccagcgctt tagcaatccc
     2881 aaaactaccc ctaagaccat gaccatggcc gcccagctga tggcggccgg agctaaccag
     2941 cagctgattg tcagcaagct ggagccgcca tcgccgccga ccggttcttc gggtccgcgc
     3001 atgccagcgc cgcccaaacc ctcatcgcca aaaaagccac aggacaaagg gactttaaac
     3061 gtatcccacg ataaaaataa gccggatagc ggtgaagttg agattgcccc cggcgaaatc
     3121 catatagacg acagaggtaa cctgaagacc acagaagaat taaaagccga acttgaacgc
     3181 ctgaaaaaaa tgcgcggcga aatgactatg atgccggagc ctactccagc gccactgccg
     3241 gcggaaccgg caccagcgcc gccaccacct ccgactcctg ctccgccgcc ggcagaaccg
     3301 attccgccgg cgccgccaat cccatcggac ggcggtatga cgaccccgat tgtggtctca
     3361 ccggaaccaa agccgcacga gctgcttgac cccatggatc atagaccgac ctttaacgcg
     3421 cccttcacag ccgataccca atcggagtgg attgaccctt acagcacagt gactattgac
     3481 cctctgagtg gtaacacggg tggaccgccc ggtggcgacg gcttcgcggg taatgaaaga
     3541 gttgtccagc cgccgccgga accgccaaaa acacttct
//
LOCUS       DASPTF010000047         4829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1656169, whole genome shotgun sequence.
ACCESSION   DASPTF010000047 DASPTF010000000
VERSION     DASPTF010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656169"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VEH48_01525"
     CDS             complement(<1..667)
                     /locus_tag="VEH48_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18083.1"
                     /translation="MWQPLSKTSDSQPDSRTIDLIALLVAITGILTISSQLFSLLHLR
                     RIHIASTDAHLTILAGLSLIYLATLLNRGKRNAWLVSLPIYVYLVARNARHFLIDFPH
                     VGHYHLAAWLNLAVPGLTLVLLAVSGRLFDVRSELRSFTTALRRSIVILLVAFVYGVT
                     GFTLMDEHDFHQEITLPSAAHYTVDQFGLTTSRQITASTKRAQIFTDSLAAISLGAVF
                     YSAL"
     gene            699..1418
                     /locus_tag="VEH48_01530"
     CDS             699..1418
                     /locus_tag="VEH48_01530"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HXY18084.1"
                     /translation="MVAKTKPRTTRPKHLLAIATLAALCFNNWLLGPWLNHRLFAHNG
                     SVSEFSAVDQPHYAIFRTLDVFSGLLFILLAILLAQYISLTDKWRRVLLWSLAILGAA
                     NFMDALFTLHCSETLDVGCRIPVIISWHRLQLPDHAYSSVIISISYLTLPLSAWFYAR
                     ARELRLLTALSALAVLAAVATFISAISEYLSDKSFTVRTSGAGQEAQMLLLGVWFICL
                     AWDITSGGAKNPSGEDKLPAA"
     gene            complement(1415..1891)
                     /locus_tag="VEH48_01535"
     CDS             complement(1415..1891)
                     /locus_tag="VEH48_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18085.1"
                     /translation="MDEGRSKQTSNLAYKLFLGFVGLVVVCGLSFYGGVHYQKSKQTD
                     ISTSSGQSVASGQNGFAGGYGSRFGGQRPNIGSVTAVSSTSITIQSSFSGSSMTFGIN
                     SSTQVTENGQTVAVSNIQTGDNALVRTSGSGSTTASQIILNPGFGGRTGGSTPGST"
     gene            complement(1898..2572)
                     /locus_tag="VEH48_01540"
     CDS             complement(1898..2572)
                     /locus_tag="VEH48_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXY18086.1"
                     /translation="MLRVRDLTKSFKSGDNVVKAVDSASFEVSDGHFVSVIGRSGSGK
                     STLLSLLGALDKPSSGSIEVDGQDVSRLHNRALTRYRCKKIGFVFQNYNLVPNLTAME
                     NVMLPMEFAGVGKSERKERAQKLLDQVGLDDVKQRRKPGRLSGGEQQRVAIARALANK
                     PNLILADEPTGNLDSQNGKMIFDLLHHLSKTQHTTIIVATHDLSIAGRTDKTFQLQDG
                     KLVAVR"
     gene            complement(2572..3996)
                     /locus_tag="VEH48_01545"
     CDS             complement(2572..3996)
                     /locus_tag="VEH48_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXY18087.1"
                     /translation="MNTFTRGVRNAFRNQIRTFSIVVILGLSIGLALAMLLAHQAVEQ
                     KINSVKSSVGNTITIAPAGFSAFSQANNALTTSQLSKVSSLAHVTKLTETLTDRLTTI
                     GLAQPTFNFGGNSSSSNSNNQTSLTSPVTFNTNNSGTGPRLFVSGGGSLPSNFSPPIT
                     ILGTSDPASVSGSSISVSSGKTISGSGDTNDALVSTTMASKNNLKVGSTFTAYGSNLT
                     VAGIFSASTEGADNDIVVSLPAEQRLSGQRGDVTSAVATVDSLDNLSATTTAVKNLLG
                     SSADVESAQDQANATVQPLQSIKSVSLYSLIGAVIAGAVIILLTMIMIVRERRREIGV
                     IKAIGSSNPKVMLQFMTEAITLTIMGAVIGIALGVVAADPITRTLVNNSSGSTSTASA
                     GFPGAAGGAIQRFAGAGGSTNFVSRGRGLGVLRSNISNIHAAVGWSIILYGLAAAVVI
                     AVMGSAVASWFIAKVRPAEVMRAE"
     gene            4107..4373
                     /locus_tag="VEH48_01550"
     CDS             4107..4373
                     /locus_tag="VEH48_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18088.1"
                     /translation="MVVAVHVIIALLSIAYSSYLLFSPSRKGFFMTYALVAATIASGT
                     YLAISTHTPILKACLAGLLYISVILVLVVNAQRRFAAGKVRKPQ"
     gene            4443..>4829
                     /locus_tag="VEH48_01555"
     CDS             4443..>4829
                     /locus_tag="VEH48_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HXY18089.1"
                     /translation="MGLKYRLIIAILVVAGLALTVVRYFQHHAIAVLDPKGPVAAQER
                     NLIVFTLLLVLIVVIPVYLMTVGIAWRYRAGNKKARYSPELDGNRAAEAAWWLIPTAI
                     ISVLAVVAWRSSHTLDPFRPLKSTNKQ"
BASE COUNT         1019 a         1292 c         1356 g         1162 t
ORIGIN      
        1 cgagggccga ataaaaaacc gctcccaggc tgattgccgc cagcgaatcg gtgaatatct
       61 gtgctcgttt ggtgctggct gtaatctggc ggctggtggt caggccgaat tggtcaaccg
      121 tataatgggc ggcacttggc agagtaatct cttgatggaa atcgtgttca tccatcagcg
      181 taaatccggt aacgccgtag acaaacgcca ccagcaggat gacaattgag cggcgcaggg
      241 cggtggtgaa gctgcgcagc tcactgcgga catcgaacag cctgccgctt acggccaata
      301 gcaccaatgt taagccggga accgccagat tcagccaggc cgccaggtgg taatggccga
      361 cgtgcggaaa atcaattagg aagtgacggg cattgcgggc taccagataa acataaatag
      421 gcagtgacac cagccaggcg ttgcgcttgc cacggttcag caaagtcgct agatagatca
      481 agcttaggcc ggccagaatg gtcagatggg catcggtgct ggcgatatgg atgcgccgca
      541 gatgcagcag cgagaacaac tgactgctga tggtcaagat gccggttatc gcaaccagaa
      601 gggcaatcaa gtcaatagtc cttgaatccg gctgggaatc agatgttttg ctgaggggct
      661 gccacataga tgcttctaat gtattatagc ttctagcgat ggtagcaaaa acaaaaccgc
      721 ggacaacccg gcccaaacat ttgctggcga tcgccacact ggcggccctt tgcttcaaca
      781 attggctgct tggcccctgg ctgaaccacc ggctgtttgc ccacaacggt tcagtcagcg
      841 aattcagcgc cgttgaccag ccgcactacg cgattttccg gactctagat gttttttccg
      901 ggctgctttt catcctgctg gcaatcttgc tggcgcagta tatatccctg actgataagt
      961 ggcggcgcgt actgctgtgg agcctggcga tcttaggcgc ggccaatttc atggatgcgc
     1021 tttttactct acactgctcg gagacgctgg acgttggctg ccgaatcccg gtgatcatat
     1081 cctggcaccg cctgcaacta cccgatcacg cctacagcag cgttatcata tctatcagct
     1141 atctcacctt accgttgtcg gcctggttct acgcccgggc gagggaattg cggctgctga
     1201 ccgctctcag tgctctggcg gtcctggccg ccgtagcgac atttatttca gcgataagcg
     1261 aatacctgag tgataagtct tttactgtcc gcacgtccgg cgccggccag gaagcgcaaa
     1321 tgctgctgct tggtgtgtgg ttcatctgtc tggcatggga tatcacttcc ggtggcgcta
     1381 agaatccctc aggagaggat aagttgcctg cggcttaagt tgagccgggg gttgagccgc
     1441 cggtacggcc gccgaaaccg ggatttaaga taatctggct ggcggtggtt gagccgctac
     1501 cgctggtgcg cacaagcgcg ttatcgccgg tctggatatt gctaacggcg actgtctgac
     1561 cgttctcagt gacttgagta gagctgttga tgccaaatgt catgctggaa ccgctaaagc
     1621 tgctttggat ggtgatactg gtggaggaaa ccgcggtcac actgccgatg ttcggcctct
     1681 ggccgccaaa acgactgcca taaccaccgg caaaaccatt ttggccgctg gcgactgact
     1741 ggccgctaga tgtagaaata tcggtttgtt tagatttttg atagtggact ccgccgtaaa
     1801 aactcagacc gcaaactacc accagaccta caaaccctaa aaacagcttg taggccagat
     1861 tgctggtttg tttagaccgg ccctcatcca tcggcggtta cctgaccgcg accaattttc
     1921 catcctgcag ctgaaaggtc ttatcggttc tgccggcgat tgacaaatca tgcgtggcca
     1981 cgatgatcgt agtgtgctgg gttttggata gatggtgcag cagatcaaag atcattttgc
     2041 cgttctggct gtccaggtta ccggtaggtt cgtccgccag gattaaattg ggtttattgg
     2101 ccagagcccg ggcgatggct acgcgctgct gctcaccgcc cgaaagccgt ccgggtttgc
     2161 gccgctgctt gacatcgtcc aggccgacct ggtccagcag cttttgcgcc cgctctttac
     2221 gctcgctttt accgacaccg gcgaattcca tcggcagcat gacattctcc atcgcggtca
     2281 gattaggaac gagattataa ttctggaata cgaagccgat ctttttacaa cgatacctgg
     2341 ttagcgcgcg gttgtgcagt ctggaaacgt cctgaccgtc aacttcaatg ctaccgctac
     2401 tgggtttgtc cagtgccccc agcagcgata agagggtgct cttgccactg ccggagcggc
     2461 cgatgaccga cacgaaatgg ccgtccgaaa cttcaaacga tgcgctatcg acagctttga
     2521 ctacgttgtc tcccgattta aaagatttgg ttagatctct gactcttaac attattcggc
     2581 tctcattact tccgccggtc tgactttggc gatgaaccac gacgctaccg cgctgcccat
     2641 gacggcgatg acgactgccg ccgccaagcc ataaagaatt atgctccagc cgacggcggc
     2701 gtggatgttg gatatgttag agcgcagcac gcccagcccg cggccgcggg acacgaagtt
     2761 ggtgctgccg ccggcgccgg caaagcgctg aatagcgccg ccagcggccc ccggaaagcc
     2821 ggccgaagca gttgaagtgc tccccgagct gttattgacc agggtgcggg taatcgggtc
     2881 ggccgctacc actcccagtg caatacctat cacagctccc atgatcgtca gggtgatggc
     2941 ttcggtcatg aactgcagca tgactttagg gttactggag ccgatagcct tgatgacacc
     3001 gatctcacgg cggcgctcgc ggactatcat tatcatcgtc agcaaaatta tgacggcgcc
     3061 ggcgatgacg gcgcctatca ggctgtaaag cgagacactc tttatactct gtagcggctg
     3121 gaccgtggcg ttggcctggt cctgggcgct ctcaacgtcg gcgcttgaac caagcaggtt
     3181 ttttacggct gtggtcgtcg ccgataaatt atccagggaa tccacggtgg cgacggcgct
     3241 ggtgacgtca cccctttggc cgctcaggcg ctgttcggcc ggcagggaca ccacgatgtc
     3301 gttatcggcg ccctcggtac tggccgagaa tattccagct actgtcaggt tgctgccgta
     3361 agccgtaaac gttgagccga cttttaggtt attcttgctg gccatggtag tgctgaccag
     3421 ggcgtcgttg gtatcgccgc tgccgctgat tgtcttgccg ctgctgacgc tgattgaact
     3481 gccgctgacg ctggcggggt cgctggtgcc aagtatggtg atcggtgggc tgaaattgct
     3541 tggcagactg ccgccgccgc tgacgaacag ccgcggcccg gtgccgctat tattagtgtt
     3601 aaaagttacc ggcgaagtca gactggtctg attattactg ttgctggacg aggaattgcc
     3661 gccgaagtta aaagtcggct gggccaaacc gatggtcgtc agcctatctg tcaaagtctc
     3721 ggtaagctta gtgacgtgag ccaaagaact gaccttgctc agctggctgg tggtcaaagc
     3781 gttattggcc tgggaaaaag cactgaaacc ggccggcgcg atagtaatgg tgttgccgac
     3841 cgaacttttt accgaattga ttttttgttc taccgcttga tgggctaaca gcatcgccag
     3901 ggccaaaccg atactcaagc ccaggatcac tacaatcgag aaggtgcgga tctgattcct
     3961 gaatgcgtta cggacgcctc tggtaaatgt gttcattagc taattttact gcttcctgac
     4021 ctcaattaaa tatttccctc cttagaaaac gcttaagaac ggctcaggtt gtttatgccg
     4081 gcataattgt tataatattt actgatatgg tagtcgctgt tcacgtaatc atcgccctgc
     4141 tgagcattgc ctacagctct tatctgttgt tttcgccgtc gcgcaaaggt ttttttatga
     4201 cctatgcttt ggtagccgcg actatcgctt cgggcaccta tctggctatc agtacccata
     4261 cgccgattct aaaggcctgt ctggccggcc tgctttatat aagtgttatc cttgttctgg
     4321 tggtcaatgc tcagcggcgg ttcgcagccg gtaaagttcg caagccgcaa tagtctccgt
     4381 cgcctaaaat tgttatagtg ttaggcataa gtgttatgtc tttgggggtt tgagcgacta
     4441 ctatggggct aaaataccgg ctgattatcg cgatactggt tgtagccggt ctggcgctga
     4501 cagttgtgcg ctatttccag catcacgcca tagccgtgct ggatcccaaa ggtccggtgg
     4561 ccgcccagga acgcaatttg atagtcttca cgctgctttt ggtgctgata gtggtcatac
     4621 cggtttatct gatgactgtt ggtatcgcct ggcgctaccg cgccggcaac aaaaaagccc
     4681 gctacagtcc tgagctggac ggcaaccgcg ccgccgaagc tgcctggtgg ctgataccga
     4741 cggcgattat cagtgtactg gcggtggtcg cctggcgcag ttcgcatacg ctggacccct
     4801 tcaggccgct gaagagcact aataaacag
//
LOCUS       DASPTF010000048         7722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1801053, whole genome shotgun sequence.
ACCESSION   DASPTF010000048 DASPTF010000000
VERSION     DASPTF010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7722)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801053"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2314
                     /locus_tag="VEH48_01560"
     CDS             <1..2314
                     /locus_tag="VEH48_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXY18090.1"
                     /translation="PLADDPDTKLLAWTTTPWTLPANAALAVDEDADYAYVKLADGDS
                     TLILAKKRLELLDLRQNEYKLVKTVKGKELVGLRYQPLYPLPEGKFNAQQAKNAWQVV
                     HDANVSLEDGTGILHSAPRYGEADLAMGQKYDLPLIESVDSSGQLIYGPKEALGKFFK
                     TADEPIIADLTRKGKIFAAETTEHTYPFCWRCDTPLMYFATDTWFVEVTKVKDRLLKT
                     AEQINWVPANIKTGRWGKWLEGARDWAISRNRYWGAPLPIWVNTEDENDYIVVESIAQ
                     LKELAGPDIKVDDLHRPFIDEITFQKDGKTYKRVEEVLDCWFESGSMPISQWHYPFEN
                     KEKAEQLLTADFITEAIDQTRLWFYVQHVISTILFDKPAFKQVIVTGFIMAADGQKLS
                     KRLKNYPPVEDVFNTEGADALRLSLLARTQATETADYMRFDRAGVSDMQRNVLATLLN
                     SFKFFETYAKLDKWTPPNSLQGEPLQKNNILDQWILARLDETITAATKSADAYKIAHA
                     IEPVITLIDDLSNWYIRRSRKRFWKSENDADKNQAYATLWYCLMRVCQLLAPWTPFLP
                     DHIWRQLRASEMPESVHLSDWPSVKEPDNTSLKLLEDMTLARKIVEHGLGLRMFSSDI
                     QQQVKIRQPLGGLTYTGRKLNEDFEKIIAEEVNVKEVKNSHPEDEILKVSVDKTITPE
                     LRAEGIARDLVRFVQSARKNAGFNVEDRIQLKITSENAEITEVVTNPELRKMIFAETL
                     ATGELEGQGDHSATVKLDGSDINISVQKAS"
     gene            2359..2676
                     /gene="rplU"
                     /locus_tag="VEH48_01565"
     CDS             2359..2676
                     /gene="rplU"
                     /locus_tag="VEH48_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXY18091.1"
                     /translation="MKKAVIQTGGKQYLVAEGEELEVELLDPAQKAVFDALLVIDGES
                     VSVGAPFVEGVKVSADITEQLVKAEKVIAIRYKAKKRVHKTRGHRQRHSRIKITKITG
                     VRG"
     gene            2693..3049
                     /locus_tag="VEH48_01570"
     CDS             2693..3049
                     /locus_tag="VEH48_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HXY18092.1"
                     /translation="MPVKSFKDLIVWQKSFDLAGKIYLKTKQLPGSEKFGLSSQLQRA
                     AISITSNIAEGYERNNRGEYVQFLGMARASSAELEAQLLLVEKIYNLDISSELNLLTE
                     VQKMLRALGTKLSPKP"
     gene            3227..>7722
                     /locus_tag="VEH48_01575"
     CDS             3227..>7722
                     /locus_tag="VEH48_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HXY18093.1"
                     /translation="MGSFVFVVLLTTVIISSLGPKTQAITASTYLNFQARLYTAAGAI
                     VPDGNYNLEFKIYNADSTTGSQGSCSGACLWMATLTGGNVVKVVNGYFSVSLGAVNTV
                     GSFSTINWDQQLYLTMRVGGTGTPSWDPEMQNSGHSIVLTAVPLAFRANQLAVAGTNE
                     QVLQFASSTFGQTTTISLPDPGATTANICYSTTGGAVNGTCAPASGGSGYVQLQGSTP
                     GTAQTGNFNISGGGAGTGIATTFYATNFDVATSNTAMNIGTTNAALITLGNTTNSSFT
                     FKAKSSSTAYQFQNSSGSALLNIDTSNINNTNLVSNSSFEAALANWNALNGGAASQSA
                     TAPYEGADVLKIVDGTGAGGGGATYTGAAPPLLANGSIYTLSAYVRTDFVGAFTTFEM
                     GHADNGTAPTLVPCLTAQTLTTGWARFSCSWTSGAITGNPFIYFKQTDTAVSRNIYVD
                     DVLLQTDANSQPLYRNAGVSLAGTIITSPVVLQGSNNETSALQVNNSIGTQIFDIDTT
                     DSNLVLNPGFEVNQANWALKGSATALTRDTSTSYAGIASGKVTTTATAGDGLRYTFPT
                     QLPASTYTISAYVKVSAADSTFNLGYNNGADVNCASITPAASASVPSTSGWTRFVCTT
                     ATSAVLNSVYITSGTTALTSLWADSVQVEAGSTATAYGAGNINLNAVIQSPLFVRNLN
                     NSTTALTLQDSSSNTLLNVDTLNDIINLGSTGTQALASTVNIANSSGNAAQTVNVGSN
                     ANAGSAVVIDAGTTAGKLDIGNSTTAHVIRIGAGGTSAQSVTAGSTSSTSTATIQAGV
                     SGGAETLQLQVASGGVIGIGTTASTSRTIDVGSTSAVIGASTVLIASTSDATTNQVVT
                     IGSNAANTSDVTTVQGGSGTIAGTGAAIKLQPNTAGDILIGNTAGTGIITVGSSSAGQ
                     TVNIANGAGAPAVNIGNISTSGVTVSIAGANANTTLNSISIANGNTGSGGATVSILSG
                     TGTAGTGTLNLGNNTRVTTIGLGNIAPFAARTISIGSTGAQTPGVVDTINIGTAAGTV
                     AGAETINIGSGTPTGSGTNLVTIGSIASGIANQTTIQGGNGATAVSIQAASGGTILLG
                     SSANNPVQIGTGAVAGTIQIGGTGTTGTITLGQSSATSGTVTINIGSNIGAGGTQAIN
                     IGNSSTGTGNITIGNTAAGNTILQGATSIQVASLTAVTANSQVLCRDTSTHNLTSCDS
                     GAGGKPFIQGGNSFGATAVLGTNDSNSLTFETNNATQATIAVGGATTFKNSANSNVAF
                     QVQNASGSQLFNVDTTTTNLVTNPSFEQNTTNWAAKVGTETLAQTASQQYIGNDALSV
                     TTAATIASGAKFSGYTLLANTTYTLSAYVKLASGGLSSNIVYSTGTICQGSSSCTVAS
                     NTITGVGTTFTSGMVGGLLTVTSGASAGTTETVTGFTSATSITVSSSLTFAAGASYSI
                     TYNIISVGREDNGSDLNCTNINIPAITSTAWTDLSCTFTTGGTIGTNFIYIAQNDATA
                     RTFY"
BASE COUNT         2002 a         2177 c         1917 g         1626 t
ORIGIN      
        1 cccgctggcc gatgaccccg acaccaaact gctggcctgg accacgacac cgtggaccct
       61 tcccgcgaac gcagccttag ccgtggacga ggatgctgat tatgcttacg tgaaactcgc
      121 ggacggcgat tctacgctga ttttggctaa aaaacgcctg gaactgctgg atctgcgcca
      181 aaacgaatac aagctggtca aaacggtcaa aggcaaagaa ctggtcggcc tgagatacca
      241 gccgctttac cctctgccgg agggcaaatt caacgctcag caggccaaaa acgcctggca
      301 agtggtacac gacgccaacg ttagcttaga ggacggcact ggcatcctgc actcggcgcc
      361 gcgctacggc gaagccgacc tggctatggg ccaaaagtac gatttacccc ttattgagag
      421 cgtagattcc agcggccagc tgatttacgg tccaaaggaa gccctgggta agttcttcaa
      481 aaccgccgac gagccgatta tcgccgactt gacccgcaag ggcaaaatct tcgccgccga
      541 gacaaccgag cacacctatc cgttctgctg gcgctgcgac acacccctga tgtatttcgc
      601 caccgacacc tggttcgtag aagttaccaa agtcaaggac cggctgctca aaacagccga
      661 acaaatcaac tgggtgccgg ctaacatcaa gaccggccgc tggggcaagt ggctggaagg
      721 cgcccgcgat tgggcgattt cacgcaaccg ctactggggc gcgcctctgc ccatctgggt
      781 caacacggag gacgaaaacg actacatcgt agtggagagc atcgcccagc ttaaggagtt
      841 agccgggccg gatatcaagg tagacgacct gcaccggcca ttcatagatg agattacctt
      901 ccaaaaagac ggcaaaacct acaaacgggt agaagaggta ctggattgct ggtttgaatc
      961 cggctccatg cccatctccc agtggcatta cccctttgaa aacaaagaaa aggccgagca
     1021 gctgctgacc gccgatttca taactgaggc catcgaccag acccgcctgt ggttttacgt
     1081 ccagcacgtt atttctacta ttttatttga taaaccggcg ttcaaacagg tcatcgtaac
     1141 aggctttatt atggccgccg acggccagaa actgtccaag cgcctaaaaa actacccgcc
     1201 ggtagaggat gtctttaaca ccgagggcgc cgatgccttg cggctatctt tactggctcg
     1261 cactcaggct accgagaccg ctgattatat gcgttttgac agggcagggg tgagcgatat
     1321 gcagcgcaat gtgctagcca ccctgctcaa ctccttcaaa ttctttgaga cttacgccaa
     1381 gctggacaaa tggactcctc ccaactccct acaaggggaa cccctgcaaa aaaacaacat
     1441 cttagaccag tggattctgg cccgtttgga cgaaactatc acagccgcca ccaagagtgc
     1501 cgacgcttac aagattgccc acgccataga accggtgatt acccttatag atgatctgtc
     1561 taactggtat atccgccgct cccgcaagcg cttctggaaa agcgagaacg acgccgacaa
     1621 gaaccaggct tacgctactc tgtggtactg cctgatgcgt gtctgccagc tgctggcacc
     1681 ctggacgccg tttttacctg atcacatatg gcgccagctg cgggcaagcg aaatgccgga
     1741 atcggtgcac ctcagcgact ggccatctgt taaagagccg gataatacta gccttaaact
     1801 gctagaagat atgacacttg ctcgaaaaat agtggaacat ggacttggat taagaatgtt
     1861 ctcaagcgac atacaacaac aggttaagat acgacaacct ctgggcggtt tgacctacac
     1921 aggtagaaag ctgaatgagg actttgaaaa aatcattgcg gaagaagtaa atgtcaaaga
     1981 agttaaaaac tctcatcctg aagatgaaat ccttaaagtt tctgtagata aaactataac
     2041 tccagagctg agggccgagg ggatcgccag agatctggta cggtttgtgc aaagtgcgcg
     2101 taagaacgcc ggttttaacg tagaagaccg tatccagctg aaaataactt cggaaaacgc
     2161 cgaaataaca gaggtagtta ctaaccccga gcttagaaaa atgatttttg ctgaaactct
     2221 ggccaccggt gagctggaag ggcagggcga ccactccgca accgtcaaac tggatggcag
     2281 tgacatcaac atctctgtcc aaaaggccag ctgacccttg tagaaatctt tctaatctgt
     2341 taagctctag tttgacttat gaaaaaggct gtaatacaaa ccggtgggaa gcagtacctc
     2401 gtggctgaag gcgaggagct ggaagttgag ctgctggacc cggcgcaaaa ggccgttttt
     2461 gacgccctgc tggtaattga tggcgaaagc gtatctgttg gcgcgccgtt cgtagagggc
     2521 gtgaaggtga gcgccgacat caccgaacag ctggtgaagg ccgaaaaggt catcgccatc
     2581 cgttataaag ccaaaaaacg cgtgcacaaa acccgcggcc accggcagcg ccacagccgc
     2641 attaaaatca ccaaaataac aggggtgagg ggctagggta tagggtcgag ttgtgcctgt
     2701 taagtctttc aaagacctga tagtttggca aaaatctttt gatctggcgg gaaaaattta
     2761 tctaaaaact aagcaattac ctggttccga aaaatttggt ttatccagcc aacttcaacg
     2821 ggcagcaatt tcaattacct cgaacatagc tgagggctat gagcgtaata acagagggga
     2881 atacgtacaa tttttgggca tggcgcgagc atcgtcagct gaattagaag ctcagctttt
     2941 actagttgag aaaatttata atttggacat ctcgtctgaa ttaaatcttc ttacagaggt
     3001 acaaaaaatg cttcgcgcct taggtactaa actatcccct aaaccctaaa ccctaaaccc
     3061 tatcccctaa ggtgtggata agtcacaccg tctgttgaca atctgcaaca gggaagcgta
     3121 acctgttaat aggcttacaa aaaccaaaaa gccaaaacaa aaataaaaaa tgttcgtcca
     3181 ctatagaacg cgtaggcgcg cgcccgcgcg cgtaaaagcc ctgcttatgg gctcattcgt
     3241 gtttgtggtg ctcttgacta ctgttatcat atcgagcctc ggaccgaaaa ctcaagctat
     3301 cacggccagc acttacttga attttcaggc ccgcctttat accgctgccg gcgccattgt
     3361 accggacggc aactataact tagagtttaa gatttataac gccgacagca cgactggctc
     3421 gcagggcagc tgtagcggcg cctgtttgtg gatggcaacc ctgacgggcg gcaacgtagt
     3481 taaggtagtt aacggctatt tctcggtcag ccttggtgcc gttaacaccg tcggttcatt
     3541 cagcacaatc aactgggacc agcagctgta cctgactatg cgcgtcggtg gcaccggcac
     3601 gcccagttgg gatccagaga tgcaaaacag cggacacagc attgtgctaa cggctgtgcc
     3661 gctggccttc agggctaatc agctggcggt ggccggcact aacgagcagg tcctgcagtt
     3721 cgccagcagc acttttggcc agacgacgac catcagcctg ccggatccag gcgccacgac
     3781 cgctaacatc tgttactcaa ctaccggcgg cgccgttaat ggcacctgcg cgccagccag
     3841 cggcggcagc ggctatgtgc agttgcaggg ctccacgcct ggcacagctc agaccggcaa
     3901 ctttaacatc agtggtggtg gtgccggcac gggcattgcc acgacttttt atgcgactaa
     3961 cttcgatgtc gcgacgtcta acacggctat gaacattggt acaaccaacg cggcgctgat
     4021 tacgcttggc aataccacca actccagctt tactttcaag gccaaatcta gcagtacagc
     4081 ctaccagttc caaaattcca gtggatcggc gctgctgaat attgatacct ccaacatcaa
     4141 taacactaat cttgtatcaa actcatcgtt cgaagcggcg ctggccaact ggaatgcctt
     4201 gaacggcggc gcggcgtcgc agtccgccac agcgccatat gaaggcgccg acgtcttgaa
     4261 gattgttgac ggtaccggcg ccggaggtgg cggtgccacc tacacagggg ccgcgccacc
     4321 actactggca aacggttcaa tttatacctt gagcgcctac gtcaggaccg attttgtcgg
     4381 cgctttcact acctttgaaa tgggccacgc cgataacggc acggcgccga cactagtgcc
     4441 gtgtttgacc gcacagacgc tcaccaccgg ctgggcacgt ttttcttgca gttggacctc
     4501 cggcgccatc accggtaatc cgtttatata ttttaagcaa accgatactg cggtcagccg
     4561 caacatctac gttgacgacg tgctcttgca gaccgatgcc aactcccagc ctctgtaccg
     4621 caacgccggc gtcagtctgg ccggtaccat aattaccagc ccggtggttt tgcagggttc
     4681 caacaacgag acaagtgctt tacaagttaa taactctatc ggtacacaaa tattcgatat
     4741 tgataccacg gactctaacc tggtgttgaa tcccggtttt gaagttaacc aggccaactg
     4801 ggcactcaag ggcagtgcga ccgcattgac cagggatact tcaaccagct acgccggcat
     4861 tgcctcgggc aaggtaacaa ctacagctac cgccggcgac ggcctgcgct atactttccc
     4921 gacacagctg ccggcctcaa cctataccat cagcgcctat gtcaaggtca gtgccgctga
     4981 ctcaaccttt aacctgggtt ataacaacgg ggcggatgtt aattgcgcct ctatcactcc
     5041 ggccgcttcg gcatctgtgc cgtcaaccag cggctggacg cgctttgtct gtaccaccgc
     5101 caccagcgcg gtgcttaact cggtatacat aacctcaggc actaccgctt taacaagcct
     5161 gtgggctgac tccgttcaag tagaagccgg gtctaccgct acggcctacg gtgccggcaa
     5221 catcaacctt aacgctgtta tccagtcacc actgtttgtc cgcaacctaa acaactccac
     5281 gaccgcccta acgctccaag atagcagcag caatacacta cttaatgtcg atacccttaa
     5341 cgatataatc aatcttggct caaccgggac ccaagctttg gccagcacgg ttaatattgc
     5401 taatagcagc ggcaatgcag ctcagactgt gaatgttggt tctaatgcca atgccggtag
     5461 cgccgtggta atcgacgccg gaaccaccgc aggtaagctt gatatcggca actcaaccac
     5521 agcccacgtc atccgcattg gcgccggagg cacgagcgct caatcagtta cagctggttc
     5581 taccagctcg acgagcaccg caaccataca agccggagtt agcggtggcg ccgagacact
     5641 acagctacag gtcgcgtcag gcggagttat cggtataggc acaacggcct caaccagcag
     5701 gacgatagac gtcggctcaa ccagtgcggt aattggcgcc tcgacagttc taattgctag
     5761 cacctccgac gccaccacta accaggtagt aaccatcggc tcaaacgcgg ctaatacatc
     5821 agatgtcact accgtccagg gcggttcagg tactattgcg ggcaccggcg cggctattaa
     5881 gcttcagccc aatactgccg gagatattct aattggcaac acagccggca cgggtattat
     5941 taccgttgga tcatcgagcg ccggccaaac tgtcaatatt gccaacggcg ctggtgcgcc
     6001 ggcggtcaac attggcaata taagcacctc tggcgtgaca gtaagtatag ccggcgcaaa
     6061 cgcgaatact acacttaact cgataagcat agctaatggt aataccggct ccggcggtgc
     6121 gacagttagc atattatcag ggactggaac cgctggaacg ggcacgctca atctaggcaa
     6181 caacaccaga gtaaccacca ttggcctggg taatatcgcc ccgtttgctg cccgcacaat
     6241 ctctatcggc tctaccggtg cccagacacc gggtgtcgta gacaccatta acattggtac
     6301 ggctgccggt acggtagccg gagccgaaac catcaatatc ggttccggta cgcctaccgg
     6361 ctcgggtact aacctggtga caattggctc aattgcttca ggcatagcca accaaaccac
     6421 catccaaggc ggcaacgggg caacagcggt gtctatccaa gccgcttccg gaggtacgat
     6481 cttactaggg tccagcgcga ataacccggt gcaaatcggt actggcgcag ttgccggcac
     6541 catccaaatt ggcggaacgg gaactacggg aacaatcacg cttggccaga gcagcgcaac
     6601 cagcggcacg gtaaccatca acatcggctc caacatcggc gccggcggca ctcaggcaat
     6661 taacatcggc aacagcagta ccggtaccgg caatatcact atcggtaata ccgctgccgg
     6721 caacactatt cttcaggggg caacatctat ccaggtagcc tcgctgacag cggttactgc
     6781 caacagccag gtcctgtgtc gtgataccag cacgcataac ttgacgtcct gcgacagcgg
     6841 tgccggcggc aaacccttta tccagggtgg caacagcttt ggggctacgg ctgttctggg
     6901 caccaatgac agcaacagtc tgacatttga gaccaataat gccacacaag caactattgc
     6961 ggtcggcggc gccacaacct ttaaaaactc cgctaactcc aacgttgcct tccaggtgca
     7021 gaatgcctcc ggcagccaac tgtttaatgt tgacaccacc accaccaatc tggtgactaa
     7081 ccccagtttt gaacaaaata ccactaactg ggccgctaaa gtcggtactg agaccctggc
     7141 tcaaacggcc tcccagcaat acattggtaa cgacgcattg tccgttacca ctgccgcgac
     7201 catagccagc ggcgctaagt ttagcggcta tactctgctg gccaatacca cctacacttt
     7261 aagtgcttat gttaaattag cttctggcgg attaagttct aacatcgttt attcaacagg
     7321 aacgatctgt cagggaagca gcagttgtac ggttgccagc aatactatta cgggagtagg
     7381 cacaacattt accagcggta tggttggcgg tctgcttacc gtcaccagtg gtgccagcgc
     7441 cggtacaacc gagaccgtta ccggttttac cagcgctacc agcatcactg tttccagtag
     7501 tttgaccttc gccgccggcg ccagctacag cataacctat aacatcatat ctgttggccg
     7561 cgaagataac ggaagcgacc taaactgtac caatattaac ataccggcca ttacttctac
     7621 ggcctggact gatttaagct gcacgtttac aaccggcggt accatcggaa ctaactttat
     7681 atatattgcg caaaatgatg ctaccgcccg cactttctat at
//
LOCUS       DASPTF010000049         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_74252, whole genome shotgun sequence.
ACCESSION   DASPTF010000049 DASPTF010000000
VERSION     DASPTF010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74252"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1886)
                     /locus_tag="VEH48_01580"
     CDS             complement(186..1886)
                     /locus_tag="VEH48_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18094.1"
                     /translation="MEDQPPQQMPASPPLVPVEAKPPEPKRPARRWRRFKAWYADHKE
                     WSIPATAVILLLIIAGVPWSRYHAAGLVLKKDFTVQISDSTANTPVSGALVSLGGSSG
                     TTNGSGKVTLRSISVGSHTLVVTKKYYQDRHASVLVPILAQKTTPDITFVATGRQVLI
                     KVTNLINGQLLGGVQIAVADTTAKTAADGTATVVLPANASSQKAQLSLDGYNDADVTV
                     KISGQKIEQNDFKLTPAGSVYFLSTRSGQLDVMKANLDGSGAKTVVAASGYEEEGNTV
                     LSQSPDDKYVALVSKRTASDAQPQLYVLAASDDQLLQADSGNAIFTIYGWIGDSLIYT
                     SQRQDLPAWQQGLNRLKSYDATTGKTTLLDQSAGSDATADTNESYTLVMLTGNDVVYG
                     KIWTSDSLVSVLDGRQSSLHVIGFDGQNHQQVATYPAADNVQFVPHGAAAVYISDELP
                     DLSSTSYYDYNVGGVPKVVSLTGSQLYDNDLSYLFSPDGSKTFWSQPRDGKLALILGD
                     STGANQKTIQNLSDYSAAGWYTDNYLLLSKNGSELYISDLKAGNPIKVTDFEPGTFAL
                     "
     gene            complement(1940..2731)
                     /locus_tag="VEH48_01585"
     CDS             complement(1940..2731)
                     /locus_tag="VEH48_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18095.1"
                     /translation="MPPEDEQQPLPEAAAPREHVGPDYHRKENRSHKALIVTVILVLL
                     IGGGTAAWYQKHRSDNQKQAKAIQQLQAQQQAVANQIDSKTKSYTSPNFYLTFSYPAD
                     WTISDNGNGLMSAVSPAIKLKNAIGQSVTGQIELLIRNTTNKLSEFSAGNATAVFDSQ
                     KITYTDPTPNQRAQTYLSFLQYASSTSGLDGIYITSDNGYTRGQAIPLVDVSQENPII
                     DIVFNKCANDVCSGKTTPLSIEASSWNDAGFSTPLVNMLKSLSIT"
     gene            complement(2765..>3125)
                     /locus_tag="VEH48_01590"
     CDS             complement(2765..>3125)
                     /locus_tag="VEH48_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18096.1"
                     /translation="TTASSSAPNQPAQSTSVIQTKTDPTLGQYLATSDGMPLYTYKLD
                     TSGVSNCTGSCLSNWPAYTATSSVGNLPANVSTLKRTDDGSIQYTYKGLPLYTFTSDS
                     AGNVTGDGIENFQVAKP"
BASE COUNT          575 a          795 c          946 g          809 t
ORIGIN      
        1 gtcatatcgg aatcgtagtc tcgaatccac tttttaacct tgtcagccac ctcgccagcc
       61 tcactaacta ggcctaaagc tcggtaataa acattattgg taaccggcag cgctgttgtg
      121 agcgcttttt tctggtactc atcaagcgtc ataaaacctc cctgacttca attataccct
      181 ttgggtcaga gagcaaaggt gccgggttca aaatcggtga cttttattgg attgccggct
      241 ttgaggtcgc tgatgtagag ttcgctgccg tttttgctca gcagtaagta gttgtcggtg
      301 taccagccgg cggcgctgta gtcgctgagg ttttggatgg ttttttggtt ggcgccagtg
      361 ctgtcgccca gtatcagagc cagcttgccg tcgcgcggct ggctccagaa tgtcttggag
      421 ccgtcaggtg agaacaagta gctgagatca ttatcgtaaa gctggctgcc ggtaaggctg
      481 accacttttg gcaccccacc gacattgtag tcgtagtagc tggtgctgct tagatcgggc
      541 agttcgtcag aaatataaac ggcggctgcg ccgtgcggaa cgaattgtac gttgtcagcg
      601 gctggatagg tggcgacttg ctggtggttc tggccgtcaa agccgataac gtgcaggctg
      661 gattgccggc cgtccaggac cgataccaag ctgtcgctgg tccagatctt gccgtaaacc
      721 acgtcgtttc cggttagcat gaccagggta tagctttcgt tggtgtcggc ggtggcgtcg
      781 ctgccggcgc tttggtccag caaagtggtt ttgccggtgg tagcatcata acttttaagc
      841 ctgttgaggc cctgctgcca ggccggcagg tcctggcgct gtgaagtata gatgagcgaa
      901 tcgccgatcc agccgtagat agtaaagatg gcattgccgg aatcggcctg cagcagctgg
      961 tcgtcactgg ccgccagcac gtacagctgc ggctgggcat cactggcggt gcgtttggag
     1021 accagagcga cgtatttgtc atccggcgac tgcgatagca cggtattgcc ctcctcttcg
     1081 taaccgctgg cggccactac ggtcttggcg ccgctgccgt ccaggttggc tttcataaca
     1141 tccagctggc cgctgcgcgt gctgagaaag taaacgctgc cggccggcgt cagcttaaag
     1201 tcgttctgct caattttctg gccgctgatt ttgaccgtta catcggcgtc gttataaccg
     1261 tccagactca gttgggcttt ttggctggac gcgttggccg gcagaaccac cgtcgctgta
     1321 ccgtcggcgg cagttttagc ggtggtatcg gccaccgcaa tctgcacgcc gcccagcagc
     1381 tggccgttaa tcaggttggt gactttgata agcacctggc ggccggtggc cacaaaagtt
     1441 atgtccggcg tggttttttg ggcgagtatc ggcaccagca cgctggcgtg ccggtcctgg
     1501 taatactttt tggtaactac caacgtatgg ctgccgacac tgatgctgcg cagcgtcact
     1561 ttgccgctgc cgttggtggt gccgctgctg ccgccaagac tgaccagcgc gccgctgacc
     1621 ggcgtattgg cggtggaatc acttatctga acggtgaagt ctttttttag caccaaccct
     1681 gcggcgtggt agcggctcca gggcacaccg gcgattatca acaataaaat gaccgcggtg
     1741 gcgggaattg accactcttt gtggtcggcg taccaggcct tgaagcggcg ccagcggcgg
     1801 gcaggccgtt tgggttcagg cggcttagcc tctaccggca ctaacggcgg gctggcaggc
     1861 atttgctggg gcggctggtc ttccatagtt ctaacttatg cttaaactat agagtgccgc
     1921 ccctgttttg ccaacttttt taggtgatgc taagcgattt gagcatattc accagcggcg
     1981 tagaaaaacc ggcgtcattc caactggacg cttcaatact caggggtgtg gtcttaccgg
     2041 agcagacgtc attggcgcac ttattaaaaa cgatgtctat tatcggattt tcctggctga
     2101 catccaccag ggggatggct tggccccggg tgtagccgtt atcgcttgta atgtagattc
     2161 cgtccaggcc gctggtggaa ctggcgtatt gcaagaaact caaataggtc tgggcgcgct
     2221 ggttcggcgt ggggtcggtg taagtgattt tttgcgagtc aaagaccgcc gtggcgttgc
     2281 cggcgctgaa ttcgctgagt ttgttggtgg tgttgcgaat tagtagttca atctgaccgg
     2341 tcacgctttg acctatggca tttttaagct ttatggccgg cgaaacagcc gacataagcc
     2401 cgttgccgtt gtcgctgatc gtccaatcgg ccgggtagct gaaagtcaga taaaaattgg
     2461 gggaggtgta gcttttggtt ttggaatcaa tctggtttgc gactgcttgt tgctgggctt
     2521 gcagctgttg gatggctttg gcttgttttt gattgtcgga gcgatgtttt tggtaccagg
     2581 ccgcggtgcc gcctcctatc agcagaacca gaatcacagt aacaatcagc gccttgtgcg
     2641 atctgttctc ttttctatga tagtccgggc cgacgtgctc gcgcggcgct gcggcctcgg
     2701 gcagaggttg ctgttcgtct tccgggggca tagcttaatt gtacccctaa tactgaactg
     2761 ccgcttatgg cttggcgacc tggaagttct ctatgccgtc gccggtcacg ttgccggctg
     2821 aatcgcttgt gaaggtgtac aacggcaggc ctttgtaagt gtattggata gagccatcat
     2881 cagtccgttt gagggtgctg acattggccg gcaagttgcc caccgaactg gtagccgtat
     2941 aggccggcca gttgctcaag cacgaaccag tgcagttgct gacgccgcta gtgtccagct
     3001 tataggtgta aagcggcata ccgtcagaag tcgccaagta ttgccccagt gttgggtcgg
     3061 ttttggtctg gatgacactc gtactctggg ccggctggtt gggcgcgctg ctgctggccg
     3121 tcgta
//
LOCUS       DASPTF010000050         5287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_224302, whole genome shotgun sequence.
ACCESSION   DASPTF010000050 DASPTF010000000
VERSION     DASPTF010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224302"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VEH48_01595"
     CDS             <1..731
                     /locus_tag="VEH48_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HXY18097.1"
                     /translation="EHNDVDTRFLHINPGKISNYHYVLWYKDERTILIKHEEYDYHWP
                     RFRIIDMPRWIYFSSISQHAMEYHDQLAAWLERHEPVKLAFQPGTFQIEAGVHRLKRI
                     YARTEILALNREEATAVSGGDHNDINDLMDKLHALGPRIVLISDGHAGAYASDGTSRY
                     KMPIYPDPKPPFERTGAGDAFTSTFVAAIARGADVEGALLWAPINAMSVVQQVGAQAG
                     LLNASEINHFLRKAPHSYHPERLR"
     gene            734..1789
                     /locus_tag="VEH48_01600"
     CDS             734..1789
                     /locus_tag="VEH48_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18098.1"
                     /translation="MSRAIAKLLSKPEELVSSALKKAEDINGNPSADVRLLADNYQLT
                     RFKIAQLGLDPNDTTGEELYEALKTKFTADSLQLDKALGLSGAAPIAEKIDKAVQLVV
                     TNGHLPQRWLLKHPTAKKLLRQLPPKKLSKQLGYRSLDSLLKRENIDEIWLAARQLES
                     RTWQSKMTKAVSHLDSAAFEARRLQVLALPDKFWQGAKASRHFISDFFIGAAAVWPSG
                     LESDSHVLAIALGLLRELEKLSGSDLSGSLGDLNPALRWWLDNSCLLSSHKDGPVSLN
                     IHDLAVDHLRGRSYQARSSQHAAQALLDKLARSYEYAPEKVAAALKGIGYNLTDDTNS
                     ARELAGAHLALEYAEAE"
     gene            1796..2371
                     /locus_tag="VEH48_01605"
     CDS             1796..2371
                     /locus_tag="VEH48_01605"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18099.1"
                     /translation="MATAKYQICVSGAARGESVEQGEQLAAAAGQALARAGHALLTGA
                     TTGLPEAAARAYKKAQGHPSLGISPASSKVEHVLKYRLPTDSYDVILYTGLHYTGRDS
                     LLINSSDGVVSVGGRLGTLHEFVIAMETRTPFGLLLGAGGVSTEIEKLSAVLPNAHKD
                     LVIIETDADQLVAKLTKLIDKLHAPYRKIYS"
     gene            2414..2941
                     /locus_tag="VEH48_01610"
     CDS             2414..2941
                     /locus_tag="VEH48_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18100.1"
                     /translation="MKKQQVGFAAVEALLIVIVAGVLGATGWYTWNSKNKTDKILAQA
                     AAISQQAANAEKTQQTGGIKYSISVPASLKKQLQADYAKFKDTCSIFGSGPVTFDVSK
                     QSGNFVQVDETCANTNDTAIYTLQNGQWVITTTSAGTYSCIDVDQYHITKQLVPTCYI
                     NNWDGSQTNRANTYP"
     gene            2990..3682
                     /locus_tag="VEH48_01615"
     CDS             2990..3682
                     /locus_tag="VEH48_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18101.1"
                     /translation="MKRSQKGFAVLESLLILVIVAMVAGTGWYTWHAKNRTDKILSEA
                     SNTTQTVKASNSTTTLRLAEGQITLNPRNIWKTASGGYYSVQSGICGQGVTVTPQPKC
                     VDYAMLIPSGETFTNPDQFRVDIAVFDRTDMSGAADWLKGIEGGLIDNPQITTLTING
                     KDAARWLAVNPGGDTVTYVTYGIVSSKYGVLIGANAFYGSYYSYKNTANVDYRTYLPK
                     IDTLVQTLKIAS"
     gene            3721..>5287
                     /gene="uvrB"
                     /locus_tag="VEH48_01620"
     CDS             3721..>5287
                     /gene="uvrB"
                     /locus_tag="VEH48_01620"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HXY18102.1"
                     /translation="MSKFKLKSDYQPTGDQPAAIAKLTEGLEKGEKNQVLLGVTGSGK
                     TFTMANLIANTQKTTLVLSHNKTLAAQLYNEFRQFFPDNAVHYFVSYFDYYQPEAYVP
                     RSDTYIEKDSDINEEIDRLRHAATSALLSRKDVVIVASVSCIYGIGSVEDYDNMAVRL
                     KKGERRLRDKLLRQLTDIQYRRNDLAFERGNFRVRGDVVDVFPAGEESAYRIEFFGDE
                     VERINQINPLTGEIEKILDLLSIFPGSHYVTPEEKLKAALGKIRQEADERAAWFKKQG
                     KLLEAQRLEQRVKFDLEMLEQTGFVKGIENYSRYLTNREEGEQPATLLDYFPDDFLML
                     VDESHMTIPQVRGMYHGDRSRKEILVEYGFRLPSALDNRPLTFSEFERHMNQTVYVSA
                     TPAEYELSRSPAPVQQVIRPTGLLDPKIEVRPIEGQIDDLIAEIRSTVEKGQRALVTT
                     LTKRMAEDLAEYLQELNLKVAYLHSEIDTLERTDILRDLRLGVYDVLVGINLLREGLD
                     LPEVSLVAILDADK"
BASE COUNT         1386 a         1447 c         1385 g         1069 t
ORIGIN      
        1 tggagcataa cgacgttgat acgcgcttcc tgcatatcaa tcctggcaaa atcagcaatt
       61 atcactacgt tctgtggtat aaggacgagc gcacgatttt aataaagcac gaagaatacg
      121 attatcactg gccgcgtttc cggatcattg atatgccgcg ctggatctat ttcagctcta
      181 tcagccaaca cgccatggag taccacgatc agctggcggc ctggttggag cgccacgaac
      241 cggtcaagct ggcctttcaa cccggcactt tccagattga agccggtgtt caccggctaa
      301 agcggatata tgcccggacg gaaattctgg cactcaaccg cgaagaagcg acagcggttt
      361 ccggcggcga tcacaatgat atcaacgatt tgatggataa attgcacgct ctgggcccgc
      421 ggatcgtgct tatcagtgac ggccacgccg gcgcctacgc ctcggacggt accagccgct
      481 acaagatgcc gatctatccc gatcccaaac caccgtttga gcgcaccggc gccggcgacg
      541 ctttcacctc cacgttcgta gcggccatcg cccggggcgc cgacgtggaa ggcgccctgc
      601 tgtgggcgcc aattaacgcc atgagcgttg tccagcaggt gggcgcccaa gccggcctgc
      661 tcaacgcctc ggaaattaac catttcctgc gcaaagcgcc tcattcttat catccggaaa
      721 ggctgcgctg aagatgtcca gagccattgc caaattgctg agcaaacccg aagagctggt
      781 cagcagcgcg cttaaaaaag ccgaagatat taacggtaat cctagcgctg acgtgcgatt
      841 actggctgac aattaccagt tgacgcgctt taagatcgct caacttggct tggatcccaa
      901 tgataccacc ggtgaagagc tatacgaagc cttgaaaacc aagttcacgg ccgactcgct
      961 gcagctggac aaagccttag gtttgagcgg ggcagcgccc atagccgaga aaattgataa
     1021 agccgtgcag ctggtcgtca ccaacggcca tttaccgcaa agatggttgt taaaacatcc
     1081 aacggctaaa aagctactgc gccagctgcc gcccaaaaaa cttagcaaac agctgggcta
     1141 ccgttcgctg gactcactgc tcaagcgcga aaacatagat gagatttggc tggccgccag
     1201 gcaactggaa tccaggacct ggcagtcgaa aatgaccaaa gctgtcagcc acctggattc
     1261 ggcagctttt gaagcccggc ggctgcaggt tctggcgctg ccggataagt tctggcaggg
     1321 agccaaggcc agcaggcatt ttattagtga ctttttcatc ggcgcggccg ccgtctggcc
     1381 gtccggcctg gaatcagact cgcatgtgct ggcaatagct ctggggctgc ttcgcgaatt
     1441 ggagaaactc agcggttcag acttgtcagg ttcattgggc gacctaaacc cggccttgcg
     1501 ctggtggctg gataacagct gcctattgtc cagccacaaa gacggcccgg tgtcgctgaa
     1561 catccatgat ttggcggttg accatctgcg cggcaggagc tatcaagcca gaagcagtca
     1621 gcacgccgcc caagccctgt tggacaagtt ggcccgaagc tacgagtacg cgccggaaaa
     1681 agtggcggcg gcactgaagg gcataggtta taatttgaca gatgacacta atagcgccag
     1741 ggaactggcc ggcgcgcact tagcgctgga atacgccgag gcggagtagg cttgggtggc
     1801 aactgctaaa tatcagattt gtgtgtccgg cgcggcccgc ggcgaaagcg tagagcaagg
     1861 tgagcagctg gccgccgccg ccggccaggc gctagcccgc gccggccatg cgctgcttac
     1921 cggcgccact accggcctgc cagaagccgc cgcccgggct tataaaaagg cccaaggcca
     1981 ccctagcctc ggcatcagcc cggcctccag caaagttgaa cacgttttaa aataccgcct
     2041 gccaaccgac agctacgatg taattcttta taccgggctg cactataccg gccgcgatag
     2101 cctactgata aactcatccg acggggtagt cagcgttggc ggccggctgg gcacattgca
     2161 tgagttcgtc atcgctatgg aaacacgtac accgttcggc ttgctcttgg gcgcaggggg
     2221 cgtcagcact gagattgaaa aactgtcggc agtgctgccc aacgcccaca aagacctggt
     2281 gattatagag accgatgccg accagctggt ggccaaactc accaaactga tcgacaagct
     2341 ccacgccccc taccgcaaaa tctattctta aagtcgttgt gcatttagaa gcgtcaggct
     2401 atattcaaag cttatgaaga aacagcaagt tggctttgca gccgtagagg ccttgttgat
     2461 agtcatagtg gccggcgtcc tgggcgcgac cggttggtac acctggaaca gcaagaataa
     2521 aaccgacaaa atcctcgccc aagccgcagc catcagccag caagcggcca acgccgaaaa
     2581 aacccagcag accggaggca taaaatattc aataagtgta ccggccagcc tgaaaaaaca
     2641 gctccaagct gactacgcca agttcaaaga cacctgctcc attttcggca gcggtcccgt
     2701 gacgttcgat gtcagcaaac aatctggcaa cttcgtgcaa gttgatgaaa cctgcgccaa
     2761 caccaacgac acggcaattt acaccttaca aaatggccag tgggttatta ctaccaccag
     2821 cgccggcaca tattcgtgca ttgatgttga ccagtatcac atcaccaagc agctggtacc
     2881 aacctgctac ataaataact gggatggcag ccaaaccaac cgcgccaaca cttacccata
     2941 aactgtattt cgcttgtgtt ttcatccacc taagcttata ttcagaaccg tgaaaaggtc
     3001 acaaaaaggc tttgcagttt tagaatcttt gctgatcttg gttattgtcg ccatggtagc
     3061 tggcactggc tggtacactt ggcacgctaa aaatcgaaca gacaaaatcc tttccgaagc
     3121 ctcgaacacc actcaaactg ttaaagcaag taacagcacc acaaccttaa ggctagcaga
     3181 aggtcaaatc accttaaatc ccagaaacat ctggaaaaca gcttccggtg gctactacag
     3241 cgttcaatca ggcatatgcg gtcagggggt gacagtaacg ccccaaccca aatgcgttga
     3301 ttacgcaatg ctcattccaa gcggtgaaac ttttactaat cctgatcagt ttcgtgttga
     3361 catagcagta tttgatcgta ccgacatgag tggcgctgca gactggttga agggtataga
     3421 aggcggacta atagataacc cacagattac taccctcacg attaacggca aggatgctgc
     3481 aagatggcta gcagtcaatc caggcggcga tacagtaacc tatgtcacat atggcatagt
     3541 ctcctcaaaa tatggggtac tcattggagc taatgctttc tatggcagtt actacagcta
     3601 taaaaatacc gcaaatgtcg attaccgtac ttatctacca aagatagaca ccttagttca
     3661 aaccctaaag attgcaagtt gagtgaagcc tctctagtta tcaattgcta caattaaggg
     3721 gtgagcaagt tcaagctgaa gagtgactat cagccgacgg gcgaccagcc ggcggcgatt
     3781 gcaaagttga ccgaaggcct ggaaaaaggc gagaaaaacc aggttctatt gggtgtgacc
     3841 ggcagcggta aaactttcac tatggccaac ttgatagcca atacccagaa gaccacgctg
     3901 gttctgagcc ataacaaaac tctggctgcc caactctaca acgaattccg gcaatttttc
     3961 ccggacaacg ccgtgcacta ctttgtcagc tactttgatt attaccagcc ggaagcttac
     4021 gttcccagaa gcgacaccta catagaaaaa gacagcgata taaatgagga gattgaccgg
     4081 ctgcgccacg ccgctaccag cgccctgctg tcgcgcaaag acgtagtgat cgtggccagc
     4141 gtgtcctgca tctacggcat cggctcggta gaagattacg acaacatggc agtcaggctt
     4201 aaaaagggcg aacgccggct gcgcgataaa ctgctgcgcc agctcacaga tatccaatac
     4261 cgccgtaacg acttggcctt tgaacgaggc aatttccggg taaggggaga cgtggttgat
     4321 gttttcccag ccggcgaaga aagcgcttac cgtattgaat ttttcggtga cgaggtagag
     4381 cgcatcaatc aaataaaccc gctgacaggc gagatagaaa aaatacttga cttgttaagt
     4441 atttttccag gcagccacta cgtgacgccg gaagagaagc tcaaagctgc cctgggtaaa
     4501 atccgccagg aagccgacga acgcgccgcc tggttcaaaa agcaaggtaa acttctggaa
     4561 gcccagcgct tggagcagag agtcaagttt gacctggaaa tgctggaaca gaccggcttc
     4621 gtgaagggca ttgaaaacta tagccgctat ctgactaatc gcgaagaagg cgaacagccg
     4681 gccacactgc tggactattt tccggacgac ttcttgatgc tggtagacga atcgcacatg
     4741 accatccccc aggtgcgcgg gatgtaccac ggcgaccgct cacgcaaaga aatcctggtg
     4801 gaatacggct tccgtctgcc tagcgccctg gacaaccggc cgctgacctt cagcgagttt
     4861 gaacgccaca tgaaccagac ggtttacgtc agcgccacgc cggcggaata cgaacttagt
     4921 cgcagcccgg cgccggtcca acaggtcatc cggccgaccg gcctactgga tcccaagatt
     4981 gaagtccggc cgatagaggg gcagatagat gacctgatcg ccgagatccg cagcaccgtg
     5041 gagaaaggcc aaagggcttt ggttactacg ctgaccaaac gcatggccga ggacttggcg
     5101 gagtatttgc aagagctcaa cctcaaagtc gcttatctgc acagcgagat tgatacgctg
     5161 gagcggacgg atatcctgcg cgatctgcgg ctgggggttt atgacgtgct ggtcggcatc
     5221 aacttgctgc gcgaaggctt ggatttgccg gaagtcagct tggtggccat tttggacgca
     5281 gacaaag
//
LOCUS       DASPTF010000051         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_2093816, whole genome shotgun sequence.
ACCESSION   DASPTF010000051 DASPTF010000000
VERSION     DASPTF010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093816"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1781)
                     /locus_tag="VEH48_01625"
     CDS             complement(<1..1781)
                     /locus_tag="VEH48_01625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXY18103.1"
                     /translation="MAKPSPGNRPRRPNSRYITTRSGKVLKINRSMGERWMAMKEGKT
                     RRKAERLRGLPKSRLKRFIWRLSPRRLASYWFSRDGGIMALKIVGIGILVLFVVAVGV
                     FAYFRKDLPNVTDISGSDLGGSISYYDRTGTVLLWQDYNGVKRVPVPGNAISQYVKDA
                     TIATEDRNFYSEHGFDLKGIARAAYVDIFHRGSTQGGSTITQQLVKLTQDFGQKRSIS
                     LKAKELILAIELERTYTKDQILTGYLNAAPYGSVDYGVQAASADYFHKSAKDLTLPEA
                     AMLAAIPQSPAYYSPYDASTFDRAAFIERYDYVLDSMQQTGKITKAQAAAAKKYDILA
                     EVQPQQSLYAGIQAPYFVLAAKDQLLTQFASQTSSAASKKVGGWKVITTLDMNLQNES
                     EKLVASNLSYVERYNADEEAQVGEDVQTGQIVSLVGGVDFNNPEYGQNNYAAGIPIPP
                     GSSFKPYDYSTLINDNNNVGAGSVLYDAVGPLPGYPCTNHSTQQNGGNCLQDYDFLSP
                     GPETLRYALGGSRNIPAVKAMLESQPGDTSSDRAPSVNNVINTATAMMDNTYDNDHGL
                     KTYNCYSDVKLTQPTQCYGASAIGDGA"
     gene            1934..>3083
                     /gene="tyrS"
                     /locus_tag="VEH48_01630"
     CDS             1934..>3083
                     /gene="tyrS"
                     /locus_tag="VEH48_01630"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HXY18104.1"
                     /translation="MKLSEDLAWRGLIKDKTFADAGWLDQPRKFYLGIDASTDSLTIG
                     NLAVIMLARRLLEAGWGTVLLAGGATSLVGDPGGKTEERELKSKEEIFKNVESIKPQI
                     EKLFAGKPHEMVNNLDWLGDLKYLDFLRDVGKHYPMADLMQREFVGTRMGEAGSGISY
                     AEFSYSLLQGYDYWWLFKNKGVELQIGASDQWGNILSGVALVRKKEGKEVHALSMPLV
                     VNKETGVKFGKSEAGAIWLDPVKTTPTQMFQFWVNTPDSEVEEYLKIFTLLSKEEIEQ
                     VMEQQNADLSKRTAQYLLADKVTSLVHGGPKGGIASDITKYLTSQVSITEASAEDIRM
                     IKEELPAVSIGSRSGIWDPSIVGALVAARLAPSNSEARRLLESGAIYIN"
BASE COUNT          743 a          759 c          861 g          720 t
ORIGIN      
        1 taagcgccgt caccgatggc cgaggcgccg tagcactggg tcggctgggt cagcttgacg
       61 tccgaataac agttgtaagt ctttagcccg tgatcattat cataagtgtt gtccatcatg
      121 gcggtagctg tgttgatgac attattcaca gacggcgcgc gatcgctgct ggtatcgccg
      181 ggctgggatt ccagcatggc tttaacggcc gggatattac gcgagccgcc cagcgcgtaa
      241 cgcaaggttt caggcccagg tgacaaaaag tcgtaatctt gtaaacagtt gccgccattc
      301 tgctgagtgg aatgattagt gcacggataa ccgggcaaag gccccacagc atcgtaaagg
      361 actgagccgg caccgacgtt attgttgtcg tttatcagcg tggaataatc atagggtttg
      421 aaacttgaac caggcgggat agggataccc gcggcgtagt tgttctggcc atattcgggg
      481 ttgttaaaat ccacgccgcc gaccagggag acaatctggc cggtctggac gtcttcacca
      541 acttgcgctt cctcgtcggc attataacgc tcaacgtagg acagattgga ggcaactagt
      601 ttctccgatt cgttctgcag attcatgtcc aaagtagtga tgaccttcca gccgccgacc
      661 ttcttgctgg cggcactact ggtttgtgag gcgaactggg tcagaagctg gtctttggcg
      721 gccagcacga aatacggcgc ctggataccg gcgtacaaac tctgctgcgg ctggacctca
      781 gccagaatgt catacttctt ggcggccgcg gcttgggctt tggttatttt gccggtctgc
      841 tgcatggaat ccagcacgta atcgtaacgt tcaataaagg ccgccctgtc aaaagttgag
      901 gcgtcgtacg gcgaataata ggccggcgac tgcgggatgg ccgccagcat cgccgcttcc
      961 ggcagtgtca ggtctttggc gcttttgtgg aaataatcgg ccgacgccgc ctgaacgcca
     1021 taatctacgc tgccgtacgg tgcggcgttc aaataaccgg tcagaatctg gtctttggtg
     1081 taagtgcgtt ccagctcaat ggccagaatc agctctttgg ctttcaggga aatactgcgt
     1141 ttctggccga agtcctgggt cagtttgacc agctgctggg ttatagtgga gcctccctga
     1201 gtactgcccc tgtgaaagat gtctacgtag gccgcgcggg cgataccttt gaggtcaaac
     1261 ccgtgctcgc tataaaagtt gcggtcttcg gtggcgatgg tagcgtcttt cacgtattgc
     1321 gagatagcgt tgcccggtac cggcacgcgc ttgacaccat tatagtcctg ccacaataga
     1381 acagtaccgg tccggtcgta ataactgatg ctgccgccca gatcgctgcc agaaatatct
     1441 gtaacgttag gcaaatcttt gcggaaataa gcgaatacgc ctacggctac cacgaagagc
     1501 accagaatac ctatgccgac aattttcaat gccatgatgc cgccgtcacg gctgaaccaa
     1561 taggaagcca ggcgtctggg gctaagccgc catataaagc gctttagacg cgatttaggc
     1621 aggccgcgca gccgttcagc tttgcgccgg gttttacctt ctttcatagc catccagcgc
     1681 tcacccatac tgcggtttat ctttagcact ttgccgctgc gcgtggtgat ataacggctg
     1741 ttcggccggc gtgggcggtt accagggctg ggtttggcca tcaactaaaa ttccatctcc
     1801 gtttaacaat cattatagca aaatctcccc agggcataag cttgataaaa cgaatcttta
     1861 acttccggcg tcccagccgt taatcctgcg ttatactaat accagtaagg aaaattttac
     1921 tgaaaagaag cttatgaaac tgagtgaaga cctggcttgg cgcggtttga taaaagataa
     1981 aacctttgcc gacgccggct ggctggacca gccgcgcaag ttctacctgg gcattgacgc
     2041 cagcactgat agcctgacta tcggcaactt agcggtcatc atgctggccc ggcggctgtt
     2101 ggaggccggc tggggaacgg tgctgctggc cggcggcgcg acaagtttgg tcggtgaccc
     2161 cggcggcaaa acggaagaac gcgagcttaa gagtaaggaa gagattttta agaacgtaga
     2221 gagcatcaaa ccgcagatag agaagttatt tgccggtaaa ccgcacgaga tggtcaataa
     2281 tcttgattgg ctgggtgatt taaagtactt ggattttttg cgcgatgtcg gcaaacacta
     2341 cccgatggcc gacctgatgc agcgggaatt cgttggtacc cgtatgggcg aagccggcag
     2401 cggtatcagc tacgccgagt tcagctactc tctgctgcaa ggctacgact attggtggct
     2461 atttaagaat aagggcgtag agctgcagat cggcgcttct gaccagtggg ggaacatcct
     2521 ttccggcgtg gcgctggtcc gtaaaaaaga aggcaaggaa gttcatgcgc tgagtatgcc
     2581 gctggtggta aataaagaaa ccggtgtcaa atttggcaag agcgaagcag gcgccatctg
     2641 gctggatccg gtcaaaacca cgcctaccca gatgttccaa ttctgggtta ataccccaga
     2701 ttctgaggta gaagagtacc tgaaaatctt cactttgctg tccaaagagg aaatagagca
     2761 ggttatggag caacaaaacg ctgacctatc aaaacgtaca gctcaatatc ttctggctga
     2821 taaagtcaca agtctcgtac atggagggcc taagggcggt attgcttcgg atattacgaa
     2881 atacttaaca agtcaagtat ctattactga agcgtcagca gaagatataa gaatgatcaa
     2941 agaagaactc ccagcagtca gtatcggcag caggagcggg atttgggatc cctcaatcgt
     3001 aggcgcattg gtcgctgcaa ggctagcacc gtctaatagt gaggcaagac ggctgcttga
     3061 gagcggcgca atttatatta acg
//
LOCUS       DASPTF010000052         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1372991, whole genome shotgun sequence.
ACCESSION   DASPTF010000052 DASPTF010000000
VERSION     DASPTF010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372991"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /gene="rpsB"
                     /locus_tag="VEH48_01635"
     CDS             complement(<1..534)
                     /gene="rpsB"
                     /locus_tag="VEH48_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HXY18105.1"
                     /translation="MAATEVDIKKLLEAGAHFGHQASRWHPRMAPYIHSKREGSHIID
                     LTKTVEALNKALAFLSETAASGKQILLVSTKRQAREKVKEVALATGMPYVNERWLGGM
                     LTNQNTIGSRIKRLKDLEARMASGELANRYSKLEVQRFQEEIDVMNFMYGGIKEMAAK
                     PGALFVVDVLTDDIAVKE"
     gene            676..978
                     /locus_tag="VEH48_01640"
     CDS             676..978
                     /locus_tag="VEH48_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18106.1"
                     /translation="MPETLDNELIEATRRRFRYHRPTVEHVQNDSDNPNDLIYFPYDL
                     KVAASDTLGILPGVELRLVDELAKAAKQGLNNWQDITERRKQRAAEIEHRAAWRLT"
     gene            1008..1337
                     /locus_tag="VEH48_01645"
     CDS             1008..1337
                     /locus_tag="VEH48_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HXY18107.1"
                     /translation="MKKDIHPKNYRPVVFQDSNDGSTFITRSTAATEETITLDGAEYP
                     LVKVHISSSSHPFFTGEERIVDVEGRVDKFKARQKAAKAAREARAAAKPKAKKSQKQT
                     QKLGAKS"
     gene            1413..2471
                     /gene="prfA"
                     /locus_tag="VEH48_01650"
     CDS             1413..2471
                     /gene="prfA"
                     /locus_tag="VEH48_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXY18108.1"
                     /translation="MDSQEQKLRAEYAQLQQELSHPDVFGRSNYPALAKRQGELAKII
                     ALFDERDGLSSHLEEAKKMASGPDNELAELARIEAAELEQKLSTLDSSLSTLLLPKDP
                     NDGRDCIVEIRAAAGGDEASLFAGDLYRMYSRWCEKHSYKTELISESPSEAGGFKEIV
                     FMVRGDQAYKMLKFEAGVHRVQRVPVTESQGRIHTSTITVAVLPEAEETDIKIDPAEL
                     KIDVFRSSGPGGQSVNTTDSAVRITHLSTGLVVTQQDEKSQIKNREKALSVLRARLLQ
                     AKIEEEQKQLSDARRRLIGSGDRSEKIRTYNFPQDRITDHRINYSRSNLPSALAGDID
                     DIIAKLHAADLALIRESS"
BASE COUNT          643 a          682 c          659 g          549 t
ORIGIN      
        1 ctctttgaca gcgatatcat ctgttaatac gtccaccacg aacagcgcgc cgggcttggc
       61 cgccatctcc ttgattccgc cgtacataaa gttcatgaca tctatctctt cctgaaatcg
      121 ctggacttcc agcttgctgt agcggttggc cagctcgccg gaggccatcc gggcttccag
      181 gtccttgagc cgtttaatgc gcgagccgat ggtgttctgg ttagtcagca tgccgcccag
      241 ccagcgctcg ttaacgtagg gcatgccagt cgccaaggcg acttctttaa ctttttccct
      301 ggcttgacgt ttagtgctga ccagtaaaat ctgcttgccg gatgcggcgg tctcgcttaa
      361 aaaagctagc gctttgttaa gcgcctccac ggttttggtc aaatcaatga tgtggctgcc
      421 ctcgcgcttg ctgtggatat agggcgccat gcgcgggtgc cagcggctgg cctggtgacc
      481 gaaatgagcg ccggcttcca gcaatttttt gatatctacc tcagttgctg ccattaaaat
      541 ccttttactt tgcgcggaac ttacctggta aaacctacac ggtgtacacc aagaggatta
      601 tcgctccacg ctgcttaata ttgacctgtt tattgtacct ctgttgtaag aaattttcaa
      661 ctgtataata gagatatgcc tgaaacactt gataacgaat taattgaagc aaccagaagg
      721 cgattccggt atcaccgacc aaccgtcgag cacgtacaga atgattccga taatccgaat
      781 gatttgatct atttccccta cgacctcaag gtggccgcca gtgatacctt aggcatattg
      841 ccgggtgtgg agttgaggtt agtggacgaa ctagccaagg ctgcaaagca aggtctaaac
      901 aactggcaag atatcacgga acgtcgtaaa cagcgagcag ccgaaattga gcatcgcgca
      961 gcttggcgat tgacctaaca gagtaaaaaa gttatactat ccgagttatg aaaaaggata
     1021 ttcatccaaa gaattaccgg ccggtggtgt tccaagacag caacgacggc tcgactttca
     1081 ttacccgctc caccgcggct acagaagaga ctattacgct tgatggtgct gagtaccctt
     1141 tggtgaaggt gcatatttcc agcagttcgc acccgttttt caccggcgag gagcgcatcg
     1201 tggacgttga gggccgggta gataagttca aagcccgcca gaaagccgcc aaagccgccc
     1261 gcgaggcccg cgccgccgcg aaacctaagg ctaaaaagtc acaaaaacag actcaaaaac
     1321 ttggcgccaa aagttagggg ctaggggtaa gggtcaggga gctaagtttt tatcccttta
     1381 ccctacacct tcaaccctat accctattaa ttatggattc acaggagcag aaattacggg
     1441 ccgagtacgc gcagctgcag caggagttgt cgcaccctga tgtttttggc cgcagcaatt
     1501 accccgccct ggccaagcgc cagggcgaac tggctaaaat catcgccctt tttgacgaac
     1561 gggatggatt atcatcgcat ttggaggagg ccaaaaaaat ggccagcggc ccagataatg
     1621 agttggccga actagccaga attgaagcgg cggagctgga gcaaaaactc tccactcttg
     1681 actcttcact ctccactctc ctgctgccca aagaccccaa tgatgggcgc gactgcatag
     1741 tggaaatccg ggcggccgcc ggcggcgacg aggccagcct gtttgccggc gatttatacc
     1801 gaatgtacag ccgctggtgc gaaaagcaca gctataaaac cgagcttatc agcgagagtc
     1861 cgtccgaagc cggcggcttc aaggaaatag tttttatggt gcgcggcgac caggcctaca
     1921 aaatgctgaa gtttgaagcc ggggtgcacc gcgtgcagcg ggtaccggtc accgaaagcc
     1981 aggggcgtat ccataccagt acaattacag tcgcagtcct gccggaagcc gaagagaccg
     2041 atattaagat tgacccggcg gagcttaaaa ttgatgtttt ccgctccagc ggaccgggcg
     2101 gccaaagcgt caataccacc gacagtgccg tgcgcatcac tcacctctca accggcctgg
     2161 tagttaccca gcaagatgaa aaaagccaaa ttaaaaaccg cgaaaaggcg ctgagcgtac
     2221 tgcgcgcccg cctgctgcag gccaaaatcg aggaggagca aaaacaattg tctgatgccc
     2281 gccgcagatt aatcggcagt ggcgaccgca gtgagaaaat ccgcacctac aactttccgc
     2341 aggaccgcat taccgaccac cgcatcaatt attcgcgctc caacttgcct agcgccctag
     2401 cgggtgatat cgacgacatc atagctaagc tgcacgccgc tgacctggcg ctgattaggg
     2461 aaagttcatg atggacagcg cggcattctt agataattgg actgagaagc tgcaacgtgc
     2521 cgggattggt acg
//
LOCUS       DASPTF010000053         3215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1952153, whole genome shotgun sequence.
ACCESSION   DASPTF010000053 DASPTF010000000
VERSION     DASPTF010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952153"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VEH48_01655"
     CDS             complement(<1..446)
                     /locus_tag="VEH48_01655"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HXY18109.1"
                     /translation="MADKKDEPKTDSWHESNYRHNGLLIAVVGALVLLLVFVLGIGIG
                     NWRHERVVSYGPGFNMVGVPGRHMGGGYFGADGGVLNGSNRASGTVTAVNGSSFTLAG
                     YGSTTNVQTNSSTQYEGGNSVKVNDTVTAFGTTSNGTLTATLVVINP"
     gene            complement(501..1388)
                     /locus_tag="VEH48_01660"
     CDS             complement(501..1388)
                     /locus_tag="VEH48_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18110.1"
                     /translation="MSERKSREARSDVRALAATDIGRLGIGLDSAETLDPIKRKRMEY
                     ALLIGPISRGLAEYLDTYQFDKPRSHISGASLLNLVGSTVAQKIVEQLQRDPSFPITS
                     KLAYLRANDFEKPLSNNIYPLGMTIITAADSSANALYDSAAANALTAQAAGASEGRTR
                     AEFIDIVKRSTRLPLARTFAALAWQQGLDEAIYRQALQQISSAGELESLLEYDPEKAE
                     VRPTKPIENWPDFLNHSSKGGSATIGDIKPADERIGCPVSFSPRLVVRYYEHMVDLME
                     YHQSWPEVLDSSLPRIDIF"
     gene            complement(1609..1962)
                     /locus_tag="VEH48_01665"
     CDS             complement(1609..1962)
                     /locus_tag="VEH48_01665"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXY18111.1"
                     /translation="MREQAPEPSETSWAFLMADRYSQELQLRRASEDEGNGAAIVAYV
                     ESGVFEGEPVDAVRLYSAMAASSDPEVRKAVVERLDKLHRLDEEGAQLLEMMLISDTD
                     PEVKRLAYIAWNQYG"
     gene            complement(1999..2247)
                     /locus_tag="VEH48_01670"
     CDS             complement(1999..2247)
                     /locus_tag="VEH48_01670"
                     /inference="COORDINATES: protein motif:HMM:NF025601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HXY18112.1"
                     /translation="MAKKKRQEEFKVKGEDLVDKVKQLIQEGNVRRLTIKDKTGKTII
                     EMPLTVGVVGTVVAPPLAAVGAIAALVTECTISVERES"
     gene            complement(2267..2524)
                     /locus_tag="VEH48_01675"
     CDS             complement(2267..2524)
                     /locus_tag="VEH48_01675"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HXY18113.1"
                     /translation="MPVFLGVHDFGKPITKEEMEGNWKRYQESAKKHGAEAWKVYFNL
                     EAGKAYCVTEAESADVVNTAHNEIDKPTKELIEVEKLKAEK"
     gene            complement(2611..>3215)
                     /locus_tag="VEH48_01680"
     CDS             complement(2611..>3215)
                     /locus_tag="VEH48_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18114.1"
                     /translation="GEQNPFAKYVEEIHAEERREREAKLAEAERTAGAERQKYADDSL
                     GTDPESLLGKEGADWCTKFLEYMAAKGYPGSQRFGPKDYPAGAVNLLRQIFFPPQPRY
                     TGYAIGLTLSIHGYQYPGPSYPPEAAKAVIMLSANNRLRTGDGGAVNTEECKVVIVKP
                     GVVVMTSETPTGFISTGPHIFYVEKPLEIVLAEYSMGRMS"
BASE COUNT          716 a          861 c          723 g          915 t
ORIGIN      
        1 gggttgatga ccacaagcgt ggctgtcagt gtgccgttgc tggtggtgcc aaaggccgtg
       61 acggtgtcgt taacctttac gctgttacca ccttcgtact gagttgagct attggtttgg
      121 acgttggtgg tagagccgta gccggctagc gtgaagctgg agccgttgac ggctgtcacc
      181 gtgccgctgg cccggttaga gccgttaagc acgccgccgt ccgcgccaaa atagccgccc
      241 cccatatggc gcccgggcac cccaaccata ttaaatccgg ggccgtaact tacgactctc
      301 tcatgccgcc agttgccaat gcctatgccc agcacaaaaa ccagtagtag caccaaagcc
      361 ccgaccacgg ctatcaaaag gccgttgtga cgataattac tctcgtgcca gctgtctgtc
      421 ttgggctcat cctttttatc tgccatagct aaattctcct ttctctgttt tcaatgtagc
      481 tcttaactct gataagaagc ttagaatata tcaatgcgcg gcagactgct atcaagcact
      541 tcgggccatg attgatggta ctccatgagg tccaccatgt gttcgtaata acgcacaact
      601 agcctggggc taaaagatac agggcagcca atacgctcat cggccggctt tatgtctcca
      661 atcgttgcgc tgcctccttt tgagctatga tttagaaaat ccggccagtt ttcaatcggt
      721 tttgtcggtc tgacttcagc cttctcgggg tcgtattcca gcaatgattc aagctccccg
      781 gccgaggaaa tttgctgcaa ggcttgtcta taaatagctt catccaatcc ttgttgccag
      841 gcgagcgcgg caaaagtacg cgccaaaggc agccgcgtgg agcgctttac gatatcgata
      901 aattcagccc gcgtccgccc ctcactcgca ccagccgcct gggccgttaa agcgtttgcg
      961 gcagccgaat catacaaggc gtttgccgat gagtctgcgg ccgtgatgat ggtcatacct
     1021 aacggataga tgttgttgga aagtggtttt tcgaagtcgt tagcacggag atacgccagt
     1081 tttgaagtaa taggaaaact cggatctctc tgtaattgtt cgacgatttt ttgtgcgacc
     1141 gtgctgccta ccagattgag gaggctggcg ccagaaatat gcgatctagg cttatcgaat
     1201 tggtatgtgt caaggtattc ggctaaacca cgacttatcg gaccaatcag caaggcgtat
     1261 tccatccttt tacgtttgat tggatctaag gtctctgcgc tgtcaaggcc gatgcccaga
     1321 cgacctatat ctgttgcagc tagtgcccga acatcagatc gtgcctccct ggatttacgc
     1381 tcactcatcg agcatatttt aacataaaat ctgataccaa gcaatatccc ttgtcttcgt
     1441 gaacccagtg gcattaataa attatcgtgg gcagctaacc ctggagtata aaaacaaaaa
     1501 ggagctaagt ttcagggctc ctctttgccg tagcttcccc gccgaagcgg tttgctgctg
     1561 gccacctcac gtggctattt ttattatagc accagtgtct cacccgggtt agccgtattg
     1621 gttccaagct atgtaagcta gtcgctttac ctccgggtcc gtgtcagata taagcatcat
     1681 ttctagcagc tgtgcaccct cttcatctaa gcggtgtaat ttgtccagtc gttcaactac
     1741 cgcttttctt acctccgggt ccgagcttgc cgccattgcg ctgtataagc gcactgcatc
     1801 cacaggctca ccttcaaaaa ctcctgattc cacataggct acaatagccg ctccattgcc
     1861 ttcatcttca ctggctcgtc tcaactgaag ctcctgcgaa taacgatccg ccattaaaaa
     1921 tgcccaactt gtctctgatg gttcaggggc ttgctctctc atgcacccat gatgcaataa
     1981 ctggttttga aaatcaactt atgattcgcg ctcgacactg atggtgcact cggtgactag
     2041 cgctgcgatg gctccgactg cagctagtgg tggagccaca accgtgccca caacacctac
     2101 cgtcagcggc atctcaatga tggttttacc agttttgtct ttaatggtca gccgccgcac
     2161 attgccttct tgaataagct gttttacttt atcgaccagg tcctcgccct taaccttgaa
     2221 ttcttcttgt ctcttcttct tggccatatt cttgttctac actaaattat ttttcggctt
     2281 tgagtttttc gacctctatc agttctttgg tcggtttatc tatttcgttg tgagcagtat
     2341 tcactacatc agccgactca gcttcggtca cgcaataagc tttaccggcc tcaaggttga
     2401 agtaaacttt ccaggcctcg gcgccatgtt tttttgctga ttcctggtag cgtttccaat
     2461 tgccctccat ctcttctttg gttatcggct tcccaaaatc gtgcacccct aaaaacaccg
     2521 gcataattgc tcccttttca ctttactttg cacttcttat tataccccga ctattactgg
     2581 gcctcgatca gttcgattta atatctgagc ctatgacatc ctgcccatag aatattccgc
     2641 taagacgatt tctagaggtt tttctacata aaaaatatga gggccagtac ttatgaaccc
     2701 agttggcgtt tcagaagtca taacgactac ccctggtttc acaatgacta ctttgcattc
     2761 ttcggtattt acggctccgc catcgccggt tcttaatcta ttgtttgcag atagcataat
     2821 aactgccttg gcagcttcag gcgggtaact gggtcctggg tattgatacc cgtgaatact
     2881 aagggtcagt ccgatagcgt atcccgtata acgcggctgc ggagggaaaa agatttgtct
     2941 tagcaggttg acagcgccgg cgggataatc tttcgggcca aaccgttgag agccgggata
     3001 accttttgcc gccatatact caagaaattt agtacaccaa tcagctccct ctttgccaag
     3061 caaagactcg gggtcagtac ccaaggaatc atctgcatat ttttggcgtt ctgcgcctgc
     3121 cgtacgttca gcttcggcca atttagcctc tcgctcgcgt cgctcttctg catgaatttc
     3181 ttcaacgtat tttgcaaagg gattttgctc acctg
//
LOCUS       DASPTF010000054         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1952271, whole genome shotgun sequence.
ACCESSION   DASPTF010000054 DASPTF010000000
VERSION     DASPTF010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952271"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VEH48_01685"
     CDS             complement(<1..1352)
                     /locus_tag="VEH48_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18115.1"
                     /translation="MARQVNKVCQSCQKVFKGRPNAKTCSDRCRKRLQRAKTAVENEA
                     ERVIVRVEKAADEIAGKVVPSFATEAGFVGDKAGAAVLPQAARVPQAAPWAIVPEVRG
                     RQPSQSDAALANLSDPVLDSVPLPAPADVPHFKPFGGLTDLFSRRQLLAGLALVVLVG
                     VALPFWLWPKSHPQNQPAPLLSSSTNLTGQTLSLNLNTIVANGKQLTLTGPLHLQPKT
                     DSTSDLSVQNSGGTNVLVVDTVNSRVGIGAAPASGNAALQVNGNILTNGELVSTGGGF
                     SLSDLGLSFNNKLVCSANGCVSSTSLPSFPAIDVANLSYLNKDQTISGVNVFSNSGNS
                     FTGNGAGLTSLDAGNISSGTLNDSRLSSNVALLNGTGPQTFTGNDQFSGTFLAQNSAD
                     STAAFQIQNTAGSSNLFVADTLNSRIGIGKVPALGLLDVNGNIFQNGHQVCDSSNNCG
                     "
     gene            complement(1771..1885)
                     /gene="rrf"
                     /locus_tag="VEH48_01690"
     rRNA            complement(1771..1885)
                     /gene="rrf"
                     /locus_tag="VEH48_01690"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2236..>2683)
                     /locus_tag="VEH48_01695"
     rRNA            complement(2236..>2683)
                     /locus_tag="VEH48_01695"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02540"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02540"
BASE COUNT          614 a          750 c          677 g          642 t
ORIGIN      
        1 taaccgcagt tattggacga atcgcagacc tggtgaccgt tttgaaagat attgccattg
       61 acatccagaa ggccaagcgc cgggactttg ccgatgccga tacggctatt gagagtgtcg
      121 gccacgaaca aattactgga accggcagtg ttttgtatct ggaacgccgc ggtggaatca
      181 gcagagtttt gcgccagaaa agtgccgctg aattgatcgt tcccggtaaa agtctgcgga
      241 cccgtgccgt ttagcagagc cacgttagac gacaaacggc tgtcgttcaa agtgccggaa
      301 gatatattgc cggcgtccaa gctagtcaga cctgcaccat tgcccgtaaa tgaattcccg
      361 ctgttactga agacgttcac gccgctaatc gtctggtcct tgttaagata tgacagattg
      421 gccacgtcta tggccggaaa actgggcaaa gatgttgaac taacacaacc gttggcgctg
      481 cacaccagct tgttgttgaa gctcagaccc agatcgctca gcgaaaagcc gccgccggtg
      541 gacaccagct cgccgttcgt caagatattg ccgttaacct gcagagcagc gttgccgctg
      601 gccggcgccg cgccgatacc gacccggctg ttaaccgtat cgaccaccag aacattggtg
      661 ccgccgctgt tctgcacgct cagatcagaa gtggaatcag ttttaggctg cagatgcaga
      721 gggccggtga gagtcagctg tttgccattg gccacaatcg tattcaaatt aaggctaagc
      781 gtctgaccgg tgaggttggt ggaacttgac aataacggtg ccggctggtt ttgaggatga
      841 cttttgggcc acagccaaaa cggcagcgct actccgacca gtactaccag cgccagcccg
      901 gccagcagct ggcggcgact gaacagatca gtcaggccgc cgaaaggctt aaaatgcggc
      961 acgtcagccg gcgccggtag cggcacgcta tcaagcacgg ggtcgctaag attggccagc
     1021 gccgcatcac tttgtgacgg ctgcctgcct cttacctccg gcacgatggc ccacggcgcg
     1081 gcctgcggca cccgcgcggc ctgcggcagc accgcggcac cggccttatc accgacaaag
     1141 ccggcctccg tagcaaagct gggcacaact tttccggcga tttcgtcagc tgccttttca
     1201 acccggacta tcacccgctc ggcttcgttc tccacggccg tcttggcgcg ctgcaggcgc
     1261 ttacggcagc ggtccgagca ggtcttggcg ttagggcggc ccttaaaaac cttttggcaa
     1321 gactggcaga ctttgttaac ttggcgcgcc atttttattt gattgtttat ttaagaactt
     1381 atgcttttaa gtctagtgat ttgccgtgac cgtgtcaaga agaaaaagcg ggacatttaa
     1441 ggtgtttttg tgccagacgg cagtgtcacg gttttatagg tgttaatttg acccgacaag
     1501 ttcgcaatat cgtataaaat tgttgcgcaa gccgcaattt agattaggtt tatccacatc
     1561 ccggtttctg atctacgcaa gcatttactt aactgacagg cccaggcctc attaaggtct
     1621 ccattaccct ctcaaagaca aaagcccccg tttccggggg cttttgtata atttggccac
     1681 gcgccatgca tgcgctggac tgtgcagact cttcgagagc actcgtcgca taattatata
     1741 aaaacacccc tttcggggtg cttttatata atttggcctc gtgctatttt cccagggcta
     1801 aaacccaagt attgtaaccg atgaagggct taactttggt gttcgggatg ggaaccagtg
     1861 tttccccttc tccatggaga ccaacctggc tattgcaccg cccgccctgt tgcctggcaa
     1921 agccagacat accggcggac tctgggcgca tcgcgacgtc agtaacgctc cgtcactcac
     1981 tcgcagtacc tttgtacagc tcgctcgttc ctcgctactt ccttgcgctg catcccagtg
     2041 caatacccag gacggtgtcc atatgatgtc attttggtca aaagtctctc acaaaaactt
     2101 ttgcactcgt gttgtaaagc aaacgacgta atcgtccaaa tatcacttta gatgattacc
     2161 aaaagctagt caagtatgca ctttcaccct cccaggacgc atgccttcgc gggatgcgac
     2221 gcatgtgttc tggacgtaaa aagtcaattc ctctattagt acacctcggc tgaacgcgtt
     2281 accgcgctta cacctagtgc ctatcaacct gatcatcttt cagggaggta tagggaaatc
     2341 taatctagag gttagtttcg cgcttaatat gctttcagcg cttatctatg ccgcactata
     2401 gctactgggc catgccgaag gttcgacaac ccatacacca gcggtgcgtt cgtctcggtc
     2461 ctctcgtact agagacaaat cctcgcaaat ttcctatcgt ccacagcgga tataatccga
     2521 actgtctcac gacgttctaa acccagctca cgtacctctt taacgggcga acagcccgac
     2581 ccttggaagg tgctccccct ccaggatgag atgagccgac atcgaggtgc caaacagcgc
     2641 cgtctatgtg aactattggg cgctataagc ctgttatccc cgg
//
LOCUS       DASPTF010000055        21199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1817941, whole genome shotgun sequence.
ACCESSION   DASPTF010000055 DASPTF010000000
VERSION     DASPTF010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 21199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21199)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21199
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817941"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..733
                     /locus_tag="VEH48_01700"
     CDS             119..733
                     /locus_tag="VEH48_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXY18116.1"
                     /translation="MDDPQLTPAEARVKPWFAIDGDKNLRIDYPLNKSSLVYDVGGYL
                     GEWAQPIYKRYGCRIEVFEPVASFANKIEKKFKNSRKVKVYNFGLGASDRKTKINLEG
                     ASSSTLKSTSVKETIQIVAADKIIKVTDRIDLMKINIEGGEYELLEHLIETGLIKNVA
                     DIQVQFHIFVREAPQKRQRLQSRLSKTHHLTYNYPWIWENWRRK"
     gene            733..2565
                     /locus_tag="VEH48_01705"
     CDS             733..2565
                     /locus_tag="VEH48_01705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXY18117.1"
                     /translation="MTKPLVSVILPVHSAQEFLNITVDSVLLQSYVNFELLIIDCASD
                     DQTKYIVNRYRDPRIKYSAIKSSQLKDGIATGIKLARGKYIALMGQTDISLPKRLEKE
                     VEYLERHSEAELVGTNYSVIDENSRIIKAQNRRQEKSLLNWLLSHPFAVGTLMARAKI
                     YNTEAKIYPEWWRVADRHPIAILPGNLYRLRQEAGKKLTGKDSIDDDYELWLRAAGGY
                     PKAVKYPSEPIKSLLASKARPIEMLDHVEHLLKSANQIWAKTELSPTQQQLLEAQINY
                     LAKQWADIKDRLEAPPTISVVMSIYNGQKFLKAAIQSILDQTFVDFEFIIIDDGSRDS
                     TVRIVNSFNDPRIRLISQANHGLIYSLNKGIDFARASLIARQDADDISLPQRLEKELS
                     ALLSLPKAGVVGGFWQYINSLNQRSIIMVTPTKDVDLRRSLYVENPFAHGATLIRKSA
                     LKASGGYGQKFVYVEDYDLWRRIGENWQFYQVPEVLYLYRISEGSISSSNSAIQADIS
                     SRIRRDQWQKPFLAKSLRSIINDGHYYKKLNSPLARQTYQKYLDQQHSITLKLFHRGA
                     FKTGLINLLAVALLNPKYVKSLLKPAAGGFLRRLGLMELRDDIY"
     gene            2552..3670
                     /locus_tag="VEH48_01710"
     CDS             2552..3670
                     /locus_tag="VEH48_01710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXY18118.1"
                     /translation="MTSIKKATTEIQALEKKLIRHRQLWQNGKLSAQGLLDLERQIEA
                     DEVTWSSLKLKLDRKPIVSVVMSALNAQRFIKASVTSILDQSLKDFEVIFIDDGSSDD
                     TAGLIQSFNDSRIRLIHQTNHGLVHSFNKGVRLARGEFIARMDADDISLPSRFEKQLA
                     WLSARPKRALVGTFFSRVGEDFNRPLDSVMFAPFKHIDLVRSFRLVNPFGHGSVMVRK
                     SAIDKTGGYRAEYEPAEDFDLWWRIAQEWEIGLIPEVLYLWRFHKSNISIRKSRTSNN
                     SALKSVNNVWAHLGGFKSPLRIVWDAHFYRQYNIPMASSVYKMYVKQQEILAAEFFQH
                     KRLLNAWSTTLALIWLKPEFLSKVSDLKKGIVDRRIEL"
     gene            3667..4878
                     /locus_tag="VEH48_01715"
     CDS             3667..4878
                     /locus_tag="VEH48_01715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXY18119.1"
                     /translation="MKILHAVQLYYPSTGGAQEVVRQLSERMVKAGHDVTVATTKIPG
                     RPSLVHNGVKIVEFDISATSHFGAGVVTGIKGDIKKYQEFLRKEKFDVVMTYAAQQWT
                     TDLMLPILDDIKAVKVFVPCGYSALYDPIYKGYFAEMPEHLRQFDATVYLSNNYRDIN
                     FAREHGLKNIHIIPNGADENEFNDPINPQIKMRLRNQYGIGGLTIMTIANYTSEKGHR
                     ELLYVYKRLPVSKATLVSAGSFTPGLGDYDLFEQQATRINLSRKFLGKRVIMVDGSNR
                     PLVRDLLKCADIFVFLSNIEASPLVLFEAAAAGVPFVTTAAGNSAEIAEWTGGGVIVK
                     TLQRENGRVAADLKSALWQVTRLARNPERRHALGAAGHKAWQNKYTWQKITKQYLDLY
                     EDLLKDKASKK"
     gene            4875..5762
                     /locus_tag="VEH48_01720"
     CDS             4875..5762
                     /locus_tag="VEH48_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HXY18120.1"
                     /translation="MIITKLEGGHSNQLFQYAAARRLSNHLGVELFIDKWWFENVVNV
                     DSPRFYELGGYNFPQRFINRSEFALVEDKPENLKAKLYKLTKGRAKPRIRHVRQRDNG
                     FNKEVLALSDNVYLDGWWQSEKYFKDIRSILLKEIEPKTKPNVKNAKWLNQIQNSNSV
                     SLHVRRGDYVENKLTNKFHGVVAPAYYQKALAYLAKKVGQKDFKLFVFSNDIRWCKEN
                     LKFDYPTIFIDGNNPGAEDMRLMKHCKHHVLANSSFSWWGAWLNQNPDKVVIAPKVWF
                     QDKQANRETDIVPSDWIRI"
     gene            5759..6682
                     /locus_tag="VEH48_01725"
     CDS             5759..6682
                     /locus_tag="VEH48_01725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXY18121.1"
                     /translation="MKSPAVSVVMAVYNGGDYLADAVESILNQTLDDFEFIIINDGSS
                     DKSLELLTGYAKKDKRIKLLSQKNQGLVASLNRGIKAAKGKYVARQDADDRSGPKRLE
                     KQVWFLEAHPKVVVVGSGISIMDDKSRIVHRHAVLLDDPELRQELLVRSPFAHGTVMF
                     RREAAVKAGLYDPGSWPAEDFDLWLRLSAYGQLANLDDSLYMYREHPSAISVKNARLQ
                     VEKLEAVRQKAWRERGRLLPKRKIDLSAYKNLNMGQLRIERIIDNNVMVSKLAWKHGQ
                     KNLALKNMAVLAGNKLVYKKAAGKLKRKIRN"
     gene            6683..7579
                     /locus_tag="VEH48_01730"
     CDS             6683..7579
                     /locus_tag="VEH48_01730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXY18122.1"
                     /translation="MTRPDISVVIPTYQREKELVNTMRNILSQSHKNLELIVLDQSRG
                     HKPETAKALRAIRDPRFRLIKIGPPSLPAARNFGLAAAKAPIVLFLDDDIKAVKNLVK
                     CHLEAHQKHPEVSAVGGRVLQDGFPVKKEVLRFDKHGVSHGVFTASQAGFTNAFPGGN
                     HSIRISDALALGGYDTRYYRIAFREESDMSLKMIRAGMKIYYEPKAEIYHLNTVGGGL
                     RHYDNLFDTLDFYRNDLFFVMHCVGLKNIPGALHAKFMEYCHIRPLSKGLKRSLYFVI
                     GFLTALRRLWFGRQIVAKETAG"
     gene            7576..8763
                     /locus_tag="VEH48_01735"
     CDS             7576..8763
                     /locus_tag="VEH48_01735"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXY18123.1"
                     /translation="MKIGIDARPLQHETQYRGIGKALEFFLLALPDCLEPDDSLVFYL
                     DSGLPRPKILDNFPKSRTVKIATTRLGRKRYFRSFLPSFRPLKPRAGDVDVILQYDAS
                     FGVPKNVPSVVTFLDLIPVLFRGQEKQRPVKGFRKGKDALARSLYWKKYLRVLELYKN
                     ASKIVAISASSRNDLLKHMTGINPDDVAVVHLGVNAAAQTGKPGAKIKNLAAQPYLLY
                     VGGIDIRKNVVGLLETFYQLKPRHPRLRLIMVGKEFELKERLEDLGWFTVLDSQPAYA
                     KDVIIPGFVSHDDLFYLYQHTEAFVFPSRYEGFGLPVLEAMSAGSPVIAYDNSSIPEV
                     AGDAALLVEDGKSLAPAVEKLLGDPALHRRLVKKGHRQVSKFSWHKTVTDTLKIMRQL
                     VDQ"
     gene            complement(8780..9802)
                     /gene="gmd"
                     /locus_tag="VEH48_01740"
     CDS             complement(8780..9802)
                     /gene="gmd"
                     /locus_tag="VEH48_01740"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXY18124.1"
                     /translation="MAKKALITGITGQDGSYLTELLLDKGYEVYGIIRRASTFNTQRI
                     DHVYQDPHAKQTRLHLIFGDLTDTSTVYNVLTQVMPDEVYNLGAQSHVRVSFDIPEYT
                     GNSVGLGAVRVLEAIHRLGLKTKYYQAGSSEMFGKVQQVPQTERTPFYPRSPYSVAKV
                     YAHWMTVNYRESYKMFACNGILFNHESPRRGETFVTRKITRAVAQIQAGLQNKLYMGN
                     LDAKRDWGYAKEYVEAMWLMMQQNKPDDYVIATGETHSVREFLEEAFKYVGLDYKKYV
                     EFDKRYLRPAEVDVLIGDASKAKKKLGWQPKTSFKELVKIMVDADVEELNRKLSGKFD
                     QTQEVV"
     gene            9840..10958
                     /locus_tag="VEH48_01745"
     CDS             9840..10958
                     /locus_tag="VEH48_01745"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXY18125.1"
                     /translation="MSRKIRVLFDANPLARQKTGVGFFTEGIIQALTQIPELELVGHY
                     FASRGPRPKLPKADNLSFTANPWVLGQFIKALRKVRIRLPWELLSWRKADVLFFPDFT
                     TWPSLFRAPKILVIHDLTFIDCPQYVRKRNLHFLRRYVPGDARRADAILATSRYSKDR
                     LIKSFGLPPEKIIVEPIPPPKPIAPKKRISLPAKFILFIGTIEPRKNIGGLVEAYRRL
                     PEKLRTNYALVIAGGQGWDSDDVTSHIKRLQADGCDVVMTGYVDNAARAALYGKASLV
                     ALPSFYEGFGMPVLEAMSYGVPLVVSDLPVLREMVGSAVIYFNPSSVDSIKDALVKIL
                     TDDKLRENQVKKFPGLLKRYSWQTMAADLYKEIGRLAG"
     gene            10968..12062
                     /locus_tag="VEH48_01750"
     CDS             10968..12062
                     /locus_tag="VEH48_01750"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXY18126.1"
                     /translation="MRRVVIDARESGTSTGRYVDKLVENLHSLKSGLEIIILTKSPRM
                     DFFKQTAPNFKTIRCDIREFSFAEQYGLVWKIYGIRNDLVHFTMTQQPVLYFGNKVTT
                     IQDLTTTRFRNPAKNWLVFSFKQLVYKWLVKRVAKKSAYVVVPSEYVKRDVAQYAKIP
                     SSKIVVTHEAADKISEPAEPILELDSKQFIVYVGRPQPHKNLERLVEAFLLLKQTDPG
                     LKLALAGKRDVLYERLEKKIKKRGIVDIVFTGFVSEGQLRWLYENCAAYVFPSLSEGF
                     GLPGLEAMIHGAPVVSSSATCLPEIYGQAAHYFDPLDIDDMAEKIRQVINSPELRGRL
                     VKKGLAQAAKYSWANTAEQTLKIYRKALSR"
     gene            12118..13086
                     /locus_tag="VEH48_01755"
     CDS             12118..13086
                     /locus_tag="VEH48_01755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXY18127.1"
                     /translation="MSKTPGRLLSVVAPAYNEGAGILNFHKSLTEVLDKSIAGPYEII
                     YVDDGSLDDTPAKLWSLARHDKSVRVVTLSRHFGKETATTAGIHQARGAAIITIDSDG
                     QHPVGLIPKFVARWHSGSKVVIGLRVSNHKEGLVKRYGSKLFYRLFNRFTNMKLIPGS
                     TDFRLIDTSIQTEFKRMTEHNRITRGLIDWLGYKRDYIEFRASPRQAGEAGYSFKKLL
                     KLAIDSFISLSLSPLYIAAYIGAFVLPASVLLGLFMIINLALGDPLGLHATGTAYVVV
                     FVLFLVGVLLVSQGIIGLYLSHIHTETQNRPLYVVDKDRSVGLDER"
     gene            13076..13489
                     /locus_tag="VEH48_01760"
     CDS             13076..13489
                     /locus_tag="VEH48_01760"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HXY18128.1"
                     /translation="MKDKFNSLSPASRRYLAVGISVYLFELLVIVVAQKLGASATLAV
                     GISFWLGLLVSFGLQKFFTFGDKRVHHRVLIPQIAAFSLLVLFNFGFTLLVTKLLSPP
                     VPAVLTRTLALGITTIWNFYLYKTRIFKGSDELVY"
     gene            13512..16121
                     /locus_tag="VEH48_01765"
     CDS             13512..16121
                     /locus_tag="VEH48_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HXY18129.1"
                     /translation="MALTPKKISAGKHRFYSRLAARYKTFVGLPAFAKGYIVVSLAIL
                     LASTIFWSLLSARIQTRNADQLVNTYMFQHPASLSSAIFPGVHTMLFKWPLFELIKVF
                     GVSSSTFVTFTVITVLATVALLAYIIYRIERRPLIFGTICLGLASVLLLVPTQPYAGG
                     LLPVNMAMIATRNLEYILYVAAVLILIRSPSLRSPRFWLAVVLMSLLTASDRLFLMLS
                     IGGALLALVVYALSNGWNLVSLTARWLLGGLAAGIGGVLILWGINKANITHVSAQTIG
                     PYGLVHGIHDLGLGVIFASLGLFTNFGANPASSATVLREIPGEARVHLISISGLTFVI
                     NVVILVIGLFAVWHVVAASLVRNKNLKAKLDTPYTLSLVLIWTSIAGIALFALSNHDY
                     AVDARYLTIALFAVFISLASYARQQQWRPEIVSLAGLFIILGILLALPTVVKAYNNDK
                     AALAPTDERNSLIVKVLKSRPVDELVGDYWRVMPTKLASGGKLNVVPLSGCTQFRSAL
                     TSQSWQPNLSKHSFAYLLTLDGSLTDYPNCTLDQVVTSYGRPNASVLIAGSLTKPKEL
                     LLFYDHGAHKSAPVSPTNPAATVLPITLDELPHTYCSVPTVMSVVAHEDDDLLFMNPD
                     LSHDLKAGHCVRTIYITAGDSGASEFYWLAREQGSEAAYANMLGIDDIWVQRIVKLTD
                     QEFIAVANPRGNANVSLIFFHLPDGNTKGQGFSSSNFQSLAKLESGAISVEQSVDGQS
                     SYTLTQLIAALTSLMHAYQPAEIRTQSGFTGSRYRDHSDHIAVGSLVKKAYSAYEAQQ
                     YEDQVAIPLEFYIGYPVHERPANVFGSDLAQKEATFLVYSKFDGGACQSLQQCLANPA
                     YNAYLTREYQNSY"
     gene            complement(16143..17369)
                     /gene="opgC"
                     /locus_tag="VEH48_01770"
     CDS             complement(16143..17369)
                     /gene="opgC"
                     /locus_tag="VEH48_01770"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HXY18130.1"
                     /translation="MKQLKERVVAIDYFRGICILVILLSHSASFNLPYGYLSGLGRLW
                     IGAAAFFFLLSGLTLGVVRGKTVVSDFKAVLHKSWTRARDLYLLYLITALGTILIIPF
                     ITSQAMFGLTGSAPTASGFSLLVQVATFHYAPGLANFLIYYSVYMLLAPLALYVLYRY
                     RKAWPVLLLPSVALYVLNTSYPFHLLSAGIYGTFATWQLYFVLGMILARFRVAIISWY
                     YGLRQWIVKATYRLVTLSAGVALVLSAALGFNLLPHLTSLTAQGWLPAKLLDVYVRLI
                     DHKPTLDLLLMNGRTGILRPLAAVLCLAAGYLLYQKHKSAILRYSGKFVTAMGRDALW
                     IFVAQSLLIPLMSLLPLRHNLITSTAMTAALIGSMWLITQRQRLLAWVRGYAGELQAA
                     FYQAKSTAMYRQQDSS"
     gene            complement(17483..17992)
                     /locus_tag="VEH48_01775"
     CDS             complement(17483..17992)
                     /locus_tag="VEH48_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HXY18131.1"
                     /translation="MADSVNKGDIEEAIMYAKKYIEDLLSFFGLNMDVYATTEDHEVI
                     ELNIPSTHLNGFLIGAHGDTMRALQYIISSALKQQQFSHVRVNVDVAEYKKRRAERVV
                     RDAEAWFKEVQASGQPKELVPMNPADRRAVHKAATDYGLETESIGEGRERHIVLKPTG
                     EAKPNAEKQ"
     gene            complement(17992..18918)
                     /gene="yidC"
                     /locus_tag="VEH48_01780"
     CDS             complement(17992..18918)
                     /gene="yidC"
                     /locus_tag="VEH48_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXY18132.1"
                     /translation="MLTTIIVQPIFNLLVLVYALIPGHNFGLAIIIFTIIIRLLMWPL
                     VKKQLHHAKAMRELAPELKKIKAAAKGDRQKESTMTMELYKEREINPFASLGIILIQL
                     PIIIGLYSSVRRIVNTPHEIISFSYPALHHLSWLKQLSANIHRFDDSLFGLVNLGRKA
                     LDSHGFYWPALAIVALSAVAQYYQAKQLMPKSKDSRGLRQILSEAGKGKTADQAEVNA
                     AVMRNTQFLIPGVVFLFGMSFPAALPLYWLTSSGAAYLQQARILRADASEADASVKVT
                     YSKLDEKQPKDDDKKAKISAKKRAARKNRRRR"
     gene            complement(18948..19286)
                     /gene="rnpA"
                     /locus_tag="VEH48_01785"
     CDS             complement(18948..19286)
                     /gene="rnpA"
                     /locus_tag="VEH48_01785"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXY18133.1"
                     /translation="MISRTHRFHGRASLRFVHQRGKTSRGPFFLVKVALNPRRTNYRV
                     AVVVSAKVHKSAVVRNRIRRRLYSAVRQQTISQPYDIVINVFDSKVADQPPKDLLRLV
                     KRQLSAAGVL"
     gene            complement(19319..19456)
                     /gene="rpmH"
                     /locus_tag="VEH48_01790"
     CDS             complement(19319..19456)
                     /gene="rpmH"
                     /locus_tag="VEH48_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXY18134.1"
                     /translation="MPKRTYQPKKKQRSREHGFFKRMSTKTGRSVLARRRAKGRERLS
                     H"
     gene            19711..21072
                     /gene="dnaA"
                     /locus_tag="VEH48_01795"
     CDS             19711..21072
                     /gene="dnaA"
                     /locus_tag="VEH48_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXY18135.1"
                     /translation="MQEGALWQAVLGEIELSVSRGNFVTWFKNTELVRSKDNVLVVGV
                     PNIFVKNQLERKFNDLVVEVLAKNGVKPERIEYKISSVNNSAPPRDDAVLLNSQTASS
                     SQPVVSGVTHAYRQGLNERYTFDNFVVGAGNELAYAACQAIVAKPGKKYNPLFIYGGV
                     GIGKTHLIQAVGNAILAARPNTRIVYASTERFVQEFVDAVRFKKNTSGFADFYRGADV
                     LIVDDVQFLAGKERVQEEFFHTFNTLHQANKQIIMSSDKPPKDIPTLEERLRSRFAWG
                     MTIDMQNPDFETRVAILQTKASGQQVELPQPVAEFLANRVQSNIRELEGSLNQVLAFC
                     EMRSVQPSLEIVAAMFEANKNRPKHFNARLIIERTAKHFHLSMEDILGPKRDKDIVVP
                     RQIAMYMLRSELHLSFPKIARELGRKDHTTAIHSVEKIEKELGYDGPIKQYVGELKER
                     LYA"
BASE COUNT         5392 a         5244 c         5321 g         5242 t
ORIGIN      
        1 ccggcatcgc cgacaaaaga tagtctgctc atagagtccg gcgaaaagcg gctggatagg
       61 ctttgcgcag ctctgacgat aaccgttgat acggctataa gaagtttcgg agcgaaacat
      121 ggatgaccct cagcttaccc cagccgaggc tcgagtcaag ccttggttcg caattgatgg
      181 cgacaagaat ttgcgcattg attacccact taataaaagc tctcttgtct acgatgtagg
      241 cggttattta ggagaatggg cacagccgat ctataaacgt tacggctgtc gaatcgaagt
      301 atttgaaccg gttgcaagct ttgcaaacaa aatcgaaaaa aaatttaaaa atagccgcaa
      361 ggtcaaagtc tataattttg gacttggggc aagcgaccgc aaaaccaaga tcaacctcga
      421 aggagcatca tcttcaacac ttaaaagcac ctccgtcaaa gaaactattc aaatcgtggc
      481 tgccgataaa ataattaaag ttacagacag gattgacttg atgaaaatta acattgaggg
      541 tggagaatat gaactcttgg agcatctcat tgaaacaggc cttataaaaa atgttgccga
      601 tatccaagtc cagttccata tctttgttag agaagcaccg caaaagcgtc aaagactgca
      661 atctagacta tccaaaactc atcatttgac atataactac ccttggatct gggaaaattg
      721 gagacgaaaa taatgacaaa gccgcttgtc tctgtaattt taccggttca cagcgcacag
      781 gaatttctga atattaccgt agatagtgtc ttattacagt catacgtcaa cttcgaatta
      841 ctgatcattg attgcgcttc tgatgaccaa actaaataca tagttaaccg ctatcgggat
      901 ccaagaataa agtactcagc cattaagagt agtcagctta aagatggaat agcgactgga
      961 ataaagctag ctaggggcaa gtatattgcc ttaatggggc aaaccgacat ttcgctaccc
     1021 aagcgcttag aaaaagaagt cgaatacctg gagcggcact ccgaggcaga actcgtcggt
     1081 accaactata gcgtcatcga cgaaaacagt cgtatcatta aagctcagaa cagaagacaa
     1141 gaaaaaagtt tgttaaattg gctactgtct catccttttg ctgtcggtac acttatggcc
     1201 agagcaaaaa tctataatac agaagccaag atataccccg agtggtggcg ggtagctgat
     1261 cgacacccga ttgctatact gcctggaaat ctttaccgat taaggcaaga agctggtaag
     1321 aagcttacag gcaaagacag tattgatgac gattatgaac tgtggcttag ggcggctggt
     1381 ggctatccga aagctgtaaa atacccaagc gaaccgataa aatctttgct ggcatccaaa
     1441 gctcggccaa ttgagatgct tgaccatgtt gaacatttgc taaagtcggc gaatcaaatt
     1501 tgggccaaaa ctgaacttag tccgacccag caacaactac ttgaagcgca aataaactat
     1561 cttgctaagc aatgggccga tattaaagac agactggagg ctccaccaac tatttctgtg
     1621 gtaatgagca tatataacgg ccaaaaattt ttaaaagctg ccatccaaag tattctggat
     1681 caaacttttg ttgatttcga atttattatt atagacgatg gcagtagaga ctcaacagtg
     1741 cgaatagtaa attcattcaa tgaccctcga ataagactaa taagccaagc taaccacgga
     1801 ttaatatact ctttgaataa aggcatagat tttgcgcgcg cttctttgat tgccaggcag
     1861 gatgctgatg acatttcatt gccccaaagg ctggaaaaag aactaagtgc gttattgtca
     1921 ctgcctaagg ccggagtggt tggcgggttc tggcaatata tcaatagtct taaccaaaga
     1981 agcattatta tggtcacccc taccaaagat gttgatcttc gaagaagtct gtacgttgaa
     2041 aatccgtttg ctcacggtgc aacgctgatt cgaaagagcg ctctgaaagc gtccggcggc
     2101 tatggtcaaa agtttgtgta tgtcgaggat tacgatcttt ggcggagaat tggcgaaaat
     2161 tggcagtttt accaggttcc ggaagtcctc tatttatatc ggattagcga aggaagtatc
     2221 tccagcagca atagcgctat acaagctgac atttcaagtc gtatcagaag agaccagtgg
     2281 caaaaaccgt tcttagctaa gtccctgagg tcgattatca atgacggaca ttattataag
     2341 aagctgaatt cgccgctggc taggcaaact taccaaaaat acttagatca acaacactca
     2401 atcactttaa aattatttca tcgcggcgct tttaagaccg gacttataaa cttattagca
     2461 gtcgctttgc ttaatcccaa atatgtaaag tccttgctta agccagccgc tggcggtttt
     2521 ctgcgccgtc taggattaat ggagctccgt gatgacatct attaaaaaag ccacgacaga
     2581 aatccaagcg ctggagaaaa aactgatcag acatcgacaa ttgtggcaaa atggtaaatt
     2641 atcggctcaa ggattactgg atttagaaag gcaaatagag gctgatgaag ttacctggtc
     2701 ttcactcaaa ctaaagttag acagaaaacc gatagtctcg gttgtgatga gcgcgctgaa
     2761 tgctcaaaga ttcattaagg cttccgtgac tagcatcctt gaccagtcct taaaagattt
     2821 tgaggtgatt tttatcgatg acggttcttc ggatgacact gccggactta tccaaagttt
     2881 taacgattcc cgtattagac tcatccatca gactaatcac ggccttgttc atagctttaa
     2941 caaaggggtg cggctagccc gtggtgaatt catagcccgc atggatgccg atgacatttc
     3001 cttgcccagc cgtttcgaaa agcaactggc gtggttatcg gcgcgtccaa agcgcgcgct
     3061 agttggcaca tttttcagcc gcgttggtga agacttcaat cgacctttag actcagtaat
     3121 gtttgctcct tttaagcata ttgatctggt tagaagtttt aggttagtta atccctttgg
     3181 gcatggtagc gtaatggtta gaaagtctgc aattgataag actggcggct accgggctga
     3241 gtatgagccg gctgaagatt tcgatctttg gtggcgcata gctcaagaat gggaaattgg
     3301 tttaataccg gaggttttat acttatggcg ctttcataaa tcgaatattt cgattcgtaa
     3361 aagccgtaca tcaaacaact cggctttaaa atcagtcaat aatgtttggg cacatcttgg
     3421 tggttttaaa tcgcccctta gaatagtctg ggatgcccac ttttaccggc agtacaatat
     3481 tcccatggca agttcagtat ataaaatgta tgtgaagcag caggaaattt tagctgctga
     3541 atttttccaa cataaacgtc tcttgaacgc ttggagcacc actttggcct taatttggct
     3601 aaagccagaa tttttgtcta aagtatcgga tcttaagaaa ggaattgttg atcggaggat
     3661 tgaactatga aaatcttaca cgctgtacag ctctattatc cttctactgg tggcgcacaa
     3721 gaagtagtcc gccagctcag tgagcgaatg gtcaaggccg gccatgacgt taccgtcgct
     3781 actactaaaa ttcccggccg tccgagcctt gttcataatg gcgttaaaat tgttgaattt
     3841 gatatctcag ccaccagcca tttcggggca ggcgttgtta ccggtattaa aggtgacatc
     3901 aaaaaatacc aagaattttt gcggaaagaa aaattcgatg ttgtgatgac ttatgctgcc
     3961 caacagtgga cgactgattt aatgctgcca atcctggatg atatcaaagc cgtaaaagtg
     4021 tttgttcctt gcggctattc agccttgtat gaccctatat acaaaggcta ctttgccgaa
     4081 atgcccgaac atttacgtca gttcgatgcc acagtatatc tctctaacaa ttaccgtgac
     4141 attaatttcg ctagagagca cggcctaaag aacattcaca taattcctaa tggtgctgat
     4201 gagaacgaat ttaacgaccc aatcaacccg caaattaaaa tgcgcttacg caaccagtat
     4261 ggtattggcg gcttaaccat aatgacgatc gccaattata ccagcgagaa agggcacagg
     4321 gagttattgt atgtttataa acgcctgcca gttagtaagg ccaccttagt tagtgccggt
     4381 agctttacac ccgggctcgg cgattacgac ttatttgagc aacaagcaac gcgtataaac
     4441 ttaagccgga agttcttggg caaacgtgta atcatggttg atggcagtaa tcggccgctt
     4501 gttagagact tattgaagtg tgctgatatc tttgtcttct tatcgaatat tgaggctagc
     4561 cctctcgtgc tttttgaggc tgcggcggcc ggtgtgccgt tcgtgacaac agccgccggt
     4621 aacagcgctg aaatcgctga atggactggt ggaggcgtga tcgttaaaac tctccaacgc
     4681 gagaatggac gagtcgcggc ggatctaaaa agtgctttat ggcaagttac gcgcctagcg
     4741 cgtaatcctg agcgtcgtca tgccttaggc gccgccggtc ataaagcctg gcagaataaa
     4801 tacacatggc agaaaataac taagcaatac ctggacttat atgaagatct actcaaggac
     4861 aaggcaagca aaaaatgatt atcactaagc tagaaggcgg ccacagcaac cagcttttcc
     4921 aatacgctgc agctcggcgt ctttcgaatc atttgggtgt tgagcttttc attgataaat
     4981 ggtggttcga gaatgtcgtc aatgtcgata gcccgcgttt ttatgaactc ggcggttaca
     5041 acttccccca gcggttcatc aaccgcagtg aattcgcttt agtagaagat aaacccgaaa
     5101 acttaaaagc caagctttac aagcttacca agggccgcgc caaacctagg attaggcatg
     5161 tccggcaaag agacaacggt tttaataaag aagtcttagc attgtctgat aatgtgtatt
     5221 tggacggttg gtggcagagc gaaaaatact tcaaagacat ccgttcgata ctattaaaag
     5281 aaatcgagcc taagactaag cccaatgtta aaaatgccaa atggctgaat caaattcaaa
     5341 atagtaattc tgtctcgctg cacgttcgcc gcggcgacta cgtggaaaat aagctgacta
     5401 ataaattcca cggcgttgtc gcgcctgcct attatcaaaa ggcccttgcc tatctggcta
     5461 aaaaagttgg tcaaaaagac ttcaagctat tcgtgttctc caatgatatc agatggtgta
     5521 aagagaatct aaaatttgat tatccgacaa tttttatcga cggcaataat cccggtgccg
     5581 aagatatgcg gctgatgaag cattgcaaac atcatgttct ggccaacagt agcttcagct
     5641 ggtggggtgc gtggttaaac caaaacccgg ataaagtcgt tatcgcaccc aaagtctggt
     5701 tccaggataa gcaagccaac cgcgagaccg atatcgtgcc cagtgattgg atacgcatat
     5761 gaagtcgcct gcagtcagtg tagtgatggc cgtctataac ggcggcgatt atctagcgga
     5821 tgctgttgaa agcattctga accaaacgct cgatgatttt gaattcatta tcatcaatga
     5881 tggttcgagc gataaaagtt tggaactttt gaccggctac gccaaaaagg acaaacgcat
     5941 aaaattgctg agccagaaaa accaagggct ggtagcgtct ttgaaccgcg gcatcaaagc
     6001 cgccaaaggc aagtacgttg cccggcagga cgccgatgac cgctcagggc ccaaacgtct
     6061 ggagaagcaa gtttggtttt tggaggcaca cccgaaagtc gtagttgttg gatcaggaat
     6121 aagcattatg gatgataaga gccgcattgt acataggcac gcggtgctgc tggacgaccc
     6181 ggaactgcgc caggaactgc tggtgcgctc gccgttcgcc cacggcacgg tgatgtttcg
     6241 gcgggaagcg gctgtcaaag ccggtttgta tgacccaggc agctggccgg ccgaggattt
     6301 cgatctttgg ctgcgtctta gcgcctacgg tcagttagct aatttggatg attcattata
     6361 tatgtaccgc gagcacccca gcgccatctc agtcaaaaat gcccggctgc aagtggaaaa
     6421 gttggaagct gtccgccaaa aagcctggcg agaacgcggt cggctactac ccaaacgcaa
     6481 aatcgacctg tcagcttaca agaacttaaa tatggggcag ctgcgtattg aacgcatcat
     6541 tgataataac gtcatggttt ctaagctggc ctggaaacac ggccaaaaaa acctggcttt
     6601 gaaaaacatg gccgtgctgg ccggcaacaa gctggtctac aaaaaagccg ctgggaaatt
     6661 aaagcgtaaa ataaggaatt gaatgactag gcccgacatt tccgtcgtta taccgaccta
     6721 ccagcgcgag aaggaactag tgaatacgat gcgcaatatt ttgagccagt cgcataagaa
     6781 cctagagttg atagtgttgg atcaaagccg cggccacaag ccggaaaccg cgaaggcttt
     6841 gcgcgctatc agagacccgc gcttccggct cattaaaatc ggaccgccat cgttgccggc
     6901 cgctcgcaat ttcggcctag ccgccgctaa agcgccgatc gtactttttt tggatgacga
     6961 tataaaggcc gttaaaaacc tggtgaaatg tcatttggaa gcccaccaaa aacacccgga
     7021 agtcagcgcc gtcggcgggc gcgttttgca agacggcttc cccgttaaaa aagaagtctt
     7081 gcgctttgat aagcacggcg tttcgcatgg tgtgttcact gcttcgcagg ccggcttcac
     7141 taacgctttt cccggcggca accactcaat aagaattagt gacgcgctgg cgcttggcgg
     7201 ttacgatacc cgttattacc gcattgcctt ccgcgaagaa agtgatatgt cactaaaaat
     7261 gattcgcgcc ggtatgaaga tttactatga accgaaggct gagatttacc atttaaacac
     7321 agtcggtggt ggtctaagac attatgataa tctcttcgat accctggact tttaccgcaa
     7381 tgacctgttc tttgtgatgc attgcgtcgg gttgaagaac atccccggcg cgctgcatgc
     7441 caagttcatg gaatactgcc acatccggcc actaagcaag ggactgaagc gctcgcttta
     7501 ttttgttatc ggttttttga ccgctctgcg gcgcctgtgg ttcggccggc aaattgtcgc
     7561 taaggagaca gcgggatgaa aatcggcatt gacgcccggc cgctgcagca cgagacccaa
     7621 taccggggca ttggtaaagc tttggaattt ttcctgctgg ccttaccgga ttgcttggag
     7681 cccgatgaca gcctggtctt ttacctggac agcggcttgc ccaggccgaa aatcctggac
     7741 aattttccca aaagccgcac cgtcaagatt gccacgacgc gtctggggcg caagcgttac
     7801 ttccgctcgt ttctgccctc attcaggccg ctgaagccgc gagccgggga cgtggacgtc
     7861 attttgcaat acgacgcctc gttcggtgtg cccaaaaatg tgcccagcgt ggtgactttt
     7921 ctggacctta tcccggtact gttccgcggc caggaaaaac agcggccggt caaaggcttc
     7981 cgcaaaggca aggatgcctt ggcgcgcagt ttatattgga aaaaatacct gcgggtatta
     8041 gagctttaca agaacgccag caagattgtc gcgatctcag catcatccag aaacgacctt
     8101 ctgaaacata tgactggcat aaaccctgac gatgtagcgg tagttcacct tggcgtcaac
     8161 gctgccgccc aaaccggtaa gcccggtgcc aaaataaaaa atcttgccgc tcagccgtat
     8221 ttactttatg taggcggcat cgatattaga aaaaatgtcg tcggcctgtt agaaaccttc
     8281 tatcagctta agcccaggca tccaaggctc cgactcatta tggtaggtaa ggaatttgaa
     8341 ctgaaagagc gcctggagga tttaggctgg ttcactgtcc ttgatagcca gccggcttat
     8401 gccaaagatg ttatcattcc cggatttgta tcgcatgacg acctttttta cctttaccag
     8461 cacactgagg cttttgtttt ccctagccgc tacgaaggct tcggcctgcc ggtgctggaa
     8521 gccatgagcg ccggaagtcc ggttatcgcc tacgacaatt cgtctatccc ggaggttgcc
     8581 ggcgatgcgg ccttgttggt tgaagacggc aagtccttgg caccggctgt tgaaaaactt
     8641 ttgggtgacc cggccctgca ccgccggcta gtcaagaaag gacaccgcca ggtttctaaa
     8701 ttttcctggc acaagactgt taccgacacg cttaagatta tgcgacagct ggttgatcaa
     8761 taggttttgc aaagaagttt cagactactt cttgggtctg atcaaacttg ccgctgagtt
     8821 ttctgttgag ttcttcaacg tcggcgtcga ccatgatttt cactagttcc ttgaaactgg
     8881 tcttgggttg ccagcctaat tttttcttgg ctttactggc atcgccaatc agcacatcca
     8941 cttccgccgg gcgcaaatag cgcttatcga actccacgta ttttttatag tccagaccga
     9001 cgtacttaaa agcctcttct aaaaactcac ggacagagtg tgtttcgccg gtggcgataa
     9061 cgtaatcatc gggcttattc tgctgcatca tcagccacat ggcttcgacg tattctttgg
     9121 catagcccca gtcgcgttta gcgtcaaggt tccccatata cagcttgttt tgtaacccgg
     9181 cttggatttg agcaactgcg cgggtaatct tgcgggtcac gaacgtctcg ccgcgccgtg
     9241 gactttcgtg gttaaacaga atcccattgc aggcgaacat tttgtagctc tcgcggtagt
     9301 tcacggtcat ccaatgagcg taaaccttgg ccactgagta cggtgagcgc gggtagaaag
     9361 gagttcgctc ggtttgtggg acctgctgca ctttgccgaa catctcgctt gagccagcct
     9421 gataatattt ggttttcaac cccagccggt ggatcgcttc caatacacgc acagcgccca
     9481 ggccaacgga attgccggta tattccggta tgtcaaagga tacccgcaca tggctttgcg
     9541 ccccgaggtt gtaaacttca tccggcataa cctgagttaa cacattgtaa acggtgctgg
     9601 tatcagtcag gtcgccgaaa atcagatgaa gtctggtttg cttagcgtga ggatcctgat
     9661 atacgtgatc aatccgctgt gtgttaaaag tgcttgccct ccggattatc ccataaactt
     9721 cgtagccttt atctagcagc agctccgtga gataactgcc atcctggccg gtgattccgg
     9781 taattaacgc ctttttcgcc atatgaattg agtcttttat atcctatcat gagtagacga
     9841 tgagcagaaa aatccgcgtg ctgtttgacg ctaacccgct ggcccggcag aaaaccggcg
     9901 tcggcttttt cacggagggc attattcagg ctttaaccca gataccggag ctggaactgg
     9961 tcggccatta ttttgccagc cgcgggccaa gacccaaact gcccaaggcc gataatctca
    10021 gctttactgc caacccttgg gtgcttgggc aattcataaa agccttacgg aaagtgcgta
    10081 tccgattgcc ttgggagctg ctctcttggc gcaaggcgga tgtgctgttc tttccggatt
    10141 tcaccacttg gccaagccta ttcagggctc ctaaaatcct agtcatccat gacttgacct
    10201 tcattgattg cccgcagtac gttcgcaaac ggaatttaca tttcttgcgc cgctatgtgc
    10261 cgggcgacgc ccggcgagcc gatgcaatcc tggctacctc ccgctacagt aaagaccggc
    10321 tgattaaaag ttttggcttg cctcctgaaa aaatcattgt ggagccgata ccgccgccaa
    10381 agccgattgc ccccaaaaaa cgcatcagcc tgcctgcgaa attcatcctg ttcatcggca
    10441 ccattgaacc gcgcaaaaat attggcgggt tggtcgaagc ataccggcgc ctgcccgaaa
    10501 aactgcgcac aaattacgcc cttgtaatcg ccggcggaca gggttgggat tcggacgatg
    10561 tcaccagcca cataaaacgg ctgcaggctg atggctgcga cgtcgttatg accggctatg
    10621 tagataatgc tgccagggct gctctgtatg gcaaggcttc gttggtagcg ctgccatctt
    10681 tctacgaggg ctttggaatg ccggtacttg aggccatgag ctacggtgta ccgctggtcg
    10741 tgagtgactt accggtctta agagagatgg ttggcagcgc cgttatttat ttcaatccca
    10801 gctcagtgga ttcaatcaaa gatgcattgg tgaaaatcct aactgatgat aagttacgag
    10861 aaaatcaagt taaaaagttt cccggcctgc ttaaacgata cagctggcaa acaatggcgg
    10921 ctgatctcta caaagagata ggaaggctgg ctgggtagtc taggtttatg agacgggtag
    10981 tgattgatgc gcgtgagtca gggaccagca ccgggcgtta cgtagataaa ctggttgaaa
    11041 atctgcacag cttgaaaagc gggttggaga ttattatttt gacgaaaagc ccgcggatgg
    11101 attttttcaa acagactgca ccaaacttta agactatccg ctgcgacata agagaattta
    11161 gtttcgctga gcagtatggc ttagtttgga aaatctacgg tatcagaaat gatttggtgc
    11221 actttaccat gacgcagcag ccagtactct actttggtaa taaggttacg accatccagg
    11281 accttacgac gaccagattc cgcaacccgg cgaaaaattg gctggtcttc agtttcaagc
    11341 aacttgtcta taagtggctt gtcaaaaggg tggccaaaaa atccgcttat gtcgtagtgc
    11401 ccagcgaata tgtaaaacgc gatgtcgcac aatatgccaa aatcccttct tcgaagattg
    11461 tcgtgactca cgaggccgca gacaagatta gcgaaccggc tgagccgatc ctggagcttg
    11521 atagcaagca gttcattgtg tacgtcggcc ggccgcagcc gcataaaaat ctggaaaggc
    11581 tggtcgaggc ctttctgctg cttaagcaga ctgaccccgg gctcaagctg gctttggccg
    11641 gaaaacgcga tgtcctttat gagcgcctgg agaaaaaaat taaaaaacgg ggaatcgttg
    11701 acatcgtatt cacgggtttt gtcagcgaag gccagctgcg ctggctgtac gaaaactgcg
    11761 cggcctacgt ctttcccagt ttgagcgaag gtttcgggct tccgggcctg gaggcgatga
    11821 tccacggcgc gccggtcgtc agcagcagcg caacctgttt gccggaaatt tacggccaag
    11881 ccgcccatta tttcgacccg ctggatattg atgatatggc cgagaaaatc cgccaagtta
    11941 taaatagtcc ggaactacgc ggacgactgg tcaaaaaggg cttggctcaa gccgccaaat
    12001 attcctgggc caatacggca gagcaaaccc tcaaaatata tagaaaagca cttagccgat
    12061 agccccgtta atcatgaacc gctgcctacc cttttgatat acttaggttt aagataaatg
    12121 tccaagacac caggtcgcct attgtctgtc gtggcgccag cctataacga gggcgccggc
    12181 attcttaatt tccataaatc actgaccgag gtcctggata aatcaatagc cggcccttac
    12241 gagataattt atgttgacga tggcagcctc gacgacacgc cggccaaact ctggagtttg
    12301 gcaagacatg ataaatcggt tcgcgtggtc accctgtcgc gccacttcgg caaagagacc
    12361 gccaccaccg ccggcatcca tcaggcgcgg ggcgcggcga ttataaccat tgattctgac
    12421 ggccagcacc ccgttgggct gattccaaaa tttgtagctc gctggcactc cggctctaag
    12481 gtggtaatcg gcctacgcgt ctccaaccat aaagaggggc ttgtgaagcg ctacggctcg
    12541 aagctcttct accgcctgtt caaccgcttc acgaacatga agctcatccc aggctcaacc
    12601 gatttcagat tgattgacac ctcaatccag acagaattta aacggatgac tgaacataac
    12661 cgtataaccc ggggcctgat tgattggttg ggctacaaac gggattacat agagttcaga
    12721 gccagtccgc ggcaagccgg tgaggccggt tattcgttca agaaactgct taagctggca
    12781 atcgacagtt ttatttcact tagtctgtcg ccgctataca tcgcggccta catcggggct
    12841 tttgtgctgc cggcctccgt cttgctcggc ttgttcatga taatcaatct ggcactgggg
    12901 gatccgttag ggctgcacgc taccggtacc gcttacgttg tagtattcgt cctgttcctg
    12961 gtgggagtac ttttggtatc ccaaggcatc atcggtttgt acctgtcgca tatccatacc
    13021 gagacccaaa ataggccgct ttatgtcgtt gataaagaca ggtcggtggg cttggatgaa
    13081 agataaattt aactccctgt cgccggccag ccgccgctac ttggccgtcg gcatcagtgt
    13141 ttacctcttc gagctactgg tcattgttgt cgcccagaaa ctgggagcca gcgccacttt
    13201 ggcagttggc atcagcttct ggctcggcct gttggtgtcc ttcggcctgc agaagttttt
    13261 tacgttcggc gacaaacgcg tgcaccaccg cgtattgata ccacaaatcg cagccttttc
    13321 cttgctggta ctatttaact tcgggttcac gctgcttgtg accaagctac tgtcgccgcc
    13381 cgtgccggca gtgttaacac ggacgttggc attgggcata accacaattt ggaactttta
    13441 tctgtacaaa actcgcattt ttaaaggcag cgatgaactt gtatactgag agaacccgca
    13501 aaaaaacaag aatggcactc actccaaaaa aaatatcggc tggcaaacat cgattttata
    13561 gccgcctcgc tgcacgttat aagacattcg tgggcttgcc ggccttcgcc aagggctaca
    13621 tagttgtttc gctggcgata ctgctggcca gcacgatatt ttggagcctt ttaagcgccc
    13681 gcatacaaac ccgcaatgcc gaccagttgg tcaacacata catgttccag catccagcca
    13741 gcctaagcag cgccatattc cccggcgtcc acaccatgct attcaagtgg ccgctgttcg
    13801 aactcataaa agtttttgga gtatccagct ctaccttcgt cactttcacg gtgataacgg
    13861 tcttggccac ggtggctctg ctggcctata ttatttaccg cattgagcgc cggccgctga
    13921 tttttgggac tatatgttta ggcttggcat cggttctgct actggtgcca actcaaccat
    13981 atgccggcgg tctgctcccc gtgaacatgg ctatgattgc cacgcggaat ctagaatata
    14041 tattatatgt agccgccgtt cttatcctca tccgctcgcc cagcctccgc agcccgcgtt
    14101 tttggctagc cgtagtcttg atgagcttat taacggctag cgaccggttg ttcttaatgc
    14161 ttagcatagg cggagcactt ttggcgctag tcgtttatgc cttgtccaac ggctggaatt
    14221 tggtcagttt gacggctcgc tggttgcttg gcggcttggc ggccggaatc ggcggagtcc
    14281 tcattttatg gggaattaac aaagcaaata tcacccatgt ttccgctcag actatcggcc
    14341 cctacgggtt ggtccacgga atccacgatc tcgggttagg ggttatattc gcgtctctgg
    14401 gtttatttac taattttggg gctaacccgg cctctagcgc caccgttttg cgcgagattc
    14461 ctggtgaggc tcgggtacat ctgatcagca tcagcgggct gacttttgtc atcaatgtag
    14521 tgatactggt catcggccta tttgctgtct ggcacgtggt tgccgcgagt ctcgtccgca
    14581 acaagaatct gaaggccaag ctggatacgc cttacaccct ttcactggtg cttatttgga
    14641 caagtatcgc cggaatagct ctctttgctc tcagcaatca tgattatgct gttgatgccc
    14701 gttatctgac catagccctg ttcgccgttt ttatatcatt ggccagctac gctcgccaac
    14761 aacagtggcg cccggaaatt gtcagtctgg cgggtctatt cattattctc ggtatcttgc
    14821 tggctttacc gaccgtagta aaagcttaca ataacgacaa agccgctttg gcgcctaccg
    14881 acgaacgtaa ctccttgatt gtaaaagtcc taaaaagccg cccggttgat gagctggtgg
    14941 gcgattactg gcgggttatg ccaacaaagc tggcaagcgg tggcaagctt aacgtagttc
    15001 cattgtctgg ttgcacgcag ttcagaagcg ctctaaccag ccagtcctgg caacctaacc
    15061 tcagcaaaca tagttttgcc tatctattaa ctctcgatgg cagtctcaca gattatccaa
    15121 actgcacact tgatcaggtg gtgaccagct acggccggcc taacgccagt gttttgatag
    15181 ccggaagctt aaccaagcca aaagaactgc ttctgtttta tgaccacggc gcccataaaa
    15241 gcgcgcctgt cagccccacc aatccggcgg ccaccgtact acccataacg cttgatgagc
    15301 tgccgcatac ctactgttcg gtgcccactg tcatgagcgt tgtcgcccac gaagacgacg
    15361 atttgctgtt tatgaacccg gacctgtcgc acgatctgaa ggccgggcac tgcgtcagaa
    15421 caatttacat aactgccggc gactccggtg ccagcgaatt ttactggctg gcccgcgagc
    15481 aaggttccga ggcggcctat gccaacatgc tgggaattga tgacatctgg gtgcaaagaa
    15541 ttgtcaaact gaccgaccag gagttcatag ctgtggccaa tcccaggggc aacgctaacg
    15601 tgtcgctgat tttctttcat ttgcccgacg gcaataccaa gggccaaggg ttcagctcct
    15661 caaacttcca gagcttggcc aaactcgagt caggagctat ctcggtggag cagtcggttg
    15721 atggccagtc cagttataca ttaacccagt tgatagcagc cttaacctcc ttgatgcatg
    15781 cctaccaacc cgctgaaatc aggacacagt ctggtttcac aggcagcaga taccgggatc
    15841 atagcgacca catagccgtt gggagtttgg tcaaaaaagc ttatagcgca tatgaggccc
    15901 agcagtatga agaccaagtt gccatacctc ttgagtttta tatcggctac cccgttcacg
    15961 agaggccggc taacgtgttc ggcagcgacc tggcccaaaa agaagctacg ttcctggtct
    16021 actccaaatt cgatggcgga gcctgccaat ccctgcaaca atgtctcgct aatccggctt
    16081 acaatgccta cttgacccgg gagtaccaaa attcgtactg attttagact tcaggaaaat
    16141 aactagctgc tatcttgctg gcggtacatg gcggtggatt tggcttggta aaaggctgct
    16201 tgcagctcgc cggcatatcc gcggacccaa gccagcaacc tttgtcgctg ggtaatcagc
    16261 cacattgaac cgatcagcgc ggctgtcata gctgtactgg taatgagatt atgccgcagc
    16321 ggcagcaatg acatcagcgg tatcaaaagc gactgggcca caaaaatcca gagggcgtca
    16381 cgtcccatag cagtcacgaa tttgccgctg tatcgtaaaa ttgctgattt gtgcttttgg
    16441 tacagcagat agcccgccgc caagcaaagc acagccgcca gtggccgcaa tatccccgtc
    16501 cggccgttca ttaataacag gtcaagcgtt ggtttatggt caatcagccg tacgtagacg
    16561 tccagcagct tggccggcaa ccagccctgg gcggtcaggc tggtcagatg cggtaagagg
    16621 ttgaacccca aagccgccga taacaccagc gccacaccgg cactcaaagt taccagccta
    16681 taagtggcct tgacaatcca ctggcgcagc ccgtagtacc agctgatgat ggccactctg
    16741 aaacgagcca gtatcattcc cagcacaaag tatagctgcc aggtagcgaa tgtgccgtag
    16801 ataccggctg acaacaggtg gaacggataa gaagtattca gcacgtacag agcaaccgaa
    16861 ggcaacaaca acaccggcca cgcctttcta tatctgtata agacgtagag agcgagcggc
    16921 gccaaaagca tatagaccga atagtaaatt aagaagttag ccagccccgg tgcgtaatgg
    16981 aaagtcgcca cctgcaccag taggctgaag ccgctggctg tcggtgccga acccgtcagg
    17041 ccgaacatgg cctgcgaggt gataaatggg ataatcaaga tagtccccag cgctgttatc
    17101 aggtaaagca ggtacaggtc gcgggcgcgg gtccaggatt tatgcaatac cgctttaaaa
    17161 tcggatacca cggtcttgcc tctgaccacc cccagcgtca agccgctgag caggaagaaa
    17221 aaggccgcgg ccccgatcca caagcggccc agaccagaca gataaccgta aggcaggttg
    17281 aagctggcgg agtgactaag cagaatcacc aggatacaga taccccggaa ataatcaatc
    17341 gctacgactc tttcttttag ctgtttcatt ttagaacctg tatttaggat tttagcataa
    17401 aagttattaa gtactgcaaa actgaaaaaa agctgatgtt attcagctct tttacttctt
    17461 gaatatttgg ccggttgggt ttttattgct tttcggcgtt gggcttcgct tcgcctgtcg
    17521 gtttgagcac gatgtggcgt tcgcgacctt cacctatcga ttctgtttcc agaccgtagt
    17581 cggtagccgc tttgtgcacc gcccggcggt cggccggatt catcggtacc agctccttag
    17641 gctggccgct ggcctgcacc tctttgaacc aggcttcggc gtcgcggact acccgctccg
    17701 cccggcgttt tttatattca gccacatcca cgttaacccg cacatggctg aactgctgct
    17761 gcttaagggc cgagctgatg atgtactgca gggcgcgcat ggtgtcgccg tgggcaccta
    17821 tcaaaaagcc gttgaggtgg gtgctgggaa tgttcagctc gatgacctcg tggtcttctg
    17881 tagtggcgta aacgtccata ttcaggccga agaaagacag tagatcttct atgtatttct
    17941 tggcatacat aatcgcctct tcgatgtctc ctttgttaac gctgtcagcc attatcttcg
    18001 cctccggttt ttgcgggcag cccgcttttt ggccgaaatt ttggctttct tatcgtcgtc
    18061 tttcggctgt ttttcgtcca gtttactgta cgtgaccttc accgaagcat cggcttcgga
    18121 ggcatcggcc cgcaggatgc gcgcttgctg caggtaggct gcgccgctgc tggtcagcca
    18181 atacagcggc aaggccgccg gaaagctcat gccaaataag aaaactactc cgggaatcag
    18241 gaactgggtg ttgcgcatca cggcggcatt gacttcagcc tggtcggcgg tcttgccctt
    18301 accggcctca ctcaggattt gccgcaggcc tcgcgaatcc ttgcttttgg gcatcagctg
    18361 cttggcttga tagtactggg ccacggcgct taaggctacg atggccagcg ccggccagta
    18421 aaagccgtgg gagtccagag ctttgcgccc caggttgacc aggccgaaca gggagtcgtc
    18481 aaaacggtgg atgttagccg acagttgctt aagccagctc aggtggtgca gcgccgggta
    18541 agaaaagctg atgatttcgt gcggagtgtt gacgatccgc cggacactgc tgtagagccc
    18601 gatgatgatc ggcagctgga tcagtatgat gcccagactg gcaaaagggt tgatttcgcg
    18661 ctctttgtaa agctccatgg tcatggtgga ttctttttgg cggtcgcctt tggcggcggc
    18721 cttgattttc ttgagctccg gcgccagctc gcgcatggcc ttggcgtggt gcagctgctt
    18781 cttgaccagc ggccacatca gcagacggat aattatggtg aagatgatga tcgccaggcc
    18841 aaaattgtgc cccggaatca gcgcataaac cagcaccagc aaattgaaaa tcggctgaac
    18901 gataatggtc gttaacatct tagatattcg ctctctctgg ggattaacta taacacgccg
    18961 gcggcgctaa gctgcctttt aaccagcctt agcaagtctt taggcggttg gtccgcgacc
    19021 ttgctgtcaa agacattgat gacgatgtcg tatggctggc tgatcgtctg ctggcgcacg
    19081 gcggagtaca gccgccggcg gatacggttg cgcaccactg ccgatttatg gactttagcg
    19141 ctgactacca ccgccacgcg gtagttggtc cgcctgggat tgagtgctac cttgaccaga
    19201 aaaaacggcc cgcggctggt tttaccccgc tgatgcacaa agcgcaggct ggcccgcccg
    19261 tgaaagcggt gagttctgga aatcatatta atagggtata gggttaaggg gatagggtct
    19321 agtgagacaa gcgctctcgg cctttggccc gccggcgggc cagcacgctg cgtccggtct
    19381 tagtggacat acgtttaaaa aacccgtgct cgcggctacg ttgttttttc ttgggctggt
    19441 acgtcctctt aggcatgtct acactttaac ataaagtgga attgacgaca gtaaaccaca
    19501 aaaacagccg gacttttcca cggattgctt atttttgtcc acaaaataac aggtgtggct
    19561 gttctctgtg cataactttt catcttgaaa cggattttgt ttaaggcccc gtagtatttt
    19621 tgtcaaagct gtggaaaagc ccgctgcggt tagctatagt tagtgctaga aactaatcct
    19681 cttggggaaa taaagcaagg tatccacgat atgcaagaag gggcgctctg gcaagccgta
    19741 ctcggcgaaa tcgaactgag tgtctccaga gggaattttg taacgtggtt taaaaacact
    19801 gaactggtgc gcagcaaaga caatgtgctg gtggtcggcg tgccgaatat tttcgtcaaa
    19861 aaccagttgg agcgcaagtt caacgatttg gtggtcgagg tgctggccaa aaacggtgtc
    19921 aagcctgagc gcatagaata caagattagc agtgttaata attccgcgcc gccgcgggac
    19981 gacgcggttt tgctcaattc ccaaaccgcc agttctagcc agccggtcgt cagcggcgtc
    20041 acccacgcct atcgccaagg cttaaatgag cgctatacct ttgacaactt tgtggtcgga
    20101 gccggcaatg agctggctta cgccgcctgt caggcgattg tcgccaagcc gggcaaaaag
    20161 tacaacccgc tattcatcta cggcggggtg ggtatcggca aaactcattt gatccaggcg
    20221 gttggtaacg ccatcctggc cgcccggcct aacacccgga tcgtttatgc ctctaccgaa
    20281 cgctttgtgc aggaattcgt agacgccgtg cgttttaaga agaacacctc gggtttcgcg
    20341 gatttttacc gcggggccga tgtgttgatt gtggacgatg tacagtttct agctggcaag
    20401 gaacgggtgc aggaagaatt tttccacact tttaacaccc tgcaccaggc taacaaacaa
    20461 atcatcatga gctctgacaa gccgcctaag gacatcccga ctttggagga gaggctacgt
    20521 tcgcgttttg cctgggggat gaccatagac atgcagaacc cggattttga gacccgggtg
    20581 gccatcctgc agaccaaggc ttccggccag caggtagagc tgccgcagcc ggtggctgaa
    20641 tttttggcca accgcgtcca aagcaatatc cgtgagctcg aaggcagcct gaaccaagtg
    20701 ctggctttct gtgagatgcg tagcgtccag ccgagtttag aaatcgtcgc ggcgatgttt
    20761 gaggccaaca aaaaccgccc caagcatttt aatgcccggc tgatcattga gcgcaccgcc
    20821 aagcatttcc acctttcaat ggaggatatc ttggggccta agcgcgacaa agacatcgtg
    20881 gtccccaggc agatcgctat gtatatgctt agaagcgagc tgcatctaag cttccctaaa
    20941 attgcccgtg agttggggcg caaagaccac accacggcta ttcattcggt agagaaaatt
    21001 gagaaagaac ttggttacga cggaccgatc aaacaatatg tcggcgagct gaaggagcgg
    21061 ctgtatgcct agggcctgtg gacagattgt ggatggcttg cgcacaggct gtgtcaaaag
    21121 cccagagtta tcagcacaat ccacagccgg cccaaaatac ttaacaagtc aagtattttt
    21181 tatgcgcagt ttgtgcgca
//
LOCUS       DASPTF010000056         5250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_963877, whole genome shotgun sequence.
ACCESSION   DASPTF010000056 DASPTF010000000
VERSION     DASPTF010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963877"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..864
                     /locus_tag="VEH48_01800"
     CDS             130..864
                     /locus_tag="VEH48_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18136.1"
                     /translation="MAGARNRPRQSSPLNLPSAFDLFKPSKEIVLKNIWIFGPLYAVP
                     VILWVHNWIWTPAASGGSWWEKSTGVSGNWGFSPIASWGYATVGFSIIWFLLAAVGGT
                     IAQFMITVAALQASEDKALDFRELWQVVKKIGWRLLGLYLLIALYVIVGFILLIIPGI
                     IMIRRYFLAPYVMIDKNCGVTEAMELSAAMSKPYSGSIYGIIGVTILIGLIGIVPFIG
                     SLASLIVGMLYVLAPALRYQQLKKLT"
     gene            complement(856..2049)
                     /locus_tag="VEH48_01805"
     CDS             complement(856..2049)
                     /locus_tag="VEH48_01805"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HXY18137.1"
                     /translation="MLDTATALIKDVGKKLGLPDDEVDQLIAPDAEHNFEIELSNRKK
                     YPAYRVQHSNKLGPYKGGIRFHPNVDFDEVRALATLMSFKTAVAGLPMGGGKGGVVVN
                     PKELTEAELEELSRKYVRGLHQYIGPDKDVPAPDVGTNAKVIDWMVDEFERLSGDKTK
                     AGFTGKSVEKGGSLGRDAATGRGGVIILREILNKSSISGSVSFAVQGWGNVGSYFALI
                     AEKEHPRWRLVAASDSSGGAYDGNGLSAKSVDEFKKSGGRLVDLKAGKAISNDELIEL
                     DVDVLVLAALEDAVTDKNAAGVKAKMILELANGPVSYEAYKSLSGRGVIIVPDILANA
                     GGVIVSYLEWLQNKNEEYWPEDKVNRELENHLVKATDAVYELSQKQKMSLTEAAFATA
                     IKRLS"
     gene            complement(2076..3326)
                     /locus_tag="VEH48_01810"
     CDS             complement(2076..3326)
                     /locus_tag="VEH48_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18138.1"
                     /translation="MTLATIWLVPALVPGDAFAGQIVPRSLSISSGVPGATGLSYTYT
                     FTQPTVTTIQSIKFVACTTATSTYSLSGSTLGSCTAPTGLNINAGSQVGTLAGNWQNT
                     TGFTRQSSNLNDCDENGNNNVLCITRTATSPNETATSKTLTWNTQTNPTTANSTFYIG
                     MYLYSDASFNSPTDSGTVASAVVQTLTVDAAVAEILQFCIGSTAVDDATTAVGSCSSV
                     SGTTVNIGTLDPTTINISPVNAGGGNNVNGVAVLRTNAINGSSVSYDAIQASSGTNHL
                     GALRITGASCNAGNVNTDQCINSAGTTQTTFTAGTEDFGMTIGGTNCGTVTANSYYTC
                     IYSSGSEHLVPSTNYVGATGSYGTTNGFAWQESGASTQIASAATNNTVADEALILKFA
                     ATPSISTPFGSYSVQVDFTTVATY"
     gene            complement(3483..3567)
                     /locus_tag="VEH48_01815"
     tRNA            complement(3483..3567)
                     /locus_tag="VEH48_01815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3531..3533),aa:Leu,seq:tag)
     gene            3715..4383
                     /locus_tag="VEH48_01820"
     CDS             3715..4383
                     /locus_tag="VEH48_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18139.1"
                     /translation="MTNPFEERLNRVTERTRQEQLDKERALTEAAERLRVEREARAQR
                     ARLLAEEISQAKALLLRFDTPQLEYSGNYEYKETHTIPATDWIHWRYKGTRVTEPHKK
                     SRGSWFNKGAEWAQETSYTFSGWEVTATGTEGNAEPYHGARGTLVVPSDNSPMHLSIQ
                     GLKNNHFSLDEAISIGKFNWSVAVNSGYGGWRPIDHEFSVDSFLENCAEKIAAYLATK
                     QGVS"
     gene            4380..5081
                     /locus_tag="VEH48_01825"
     CDS             4380..5081
                     /locus_tag="VEH48_01825"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXY18140.1"
                     /translation="MSESEPTDRLPLTVEHIKDHLLEKLSGIGDLDIVLFGSNARGNQ
                     ISAESDIDIWISGEPETENRASELLSQDSELPAPYDVVRLGDYAGRPELESAMRFVVG
                     STGVLIAGELPSYPEDFDAEKAAAVFSAAALRQAEELIDRADIIERSGGWSNGSFSRE
                     AARSLLYAQSARTGDWASVKKLDAPALAKKLEDVDIELGSLIRTSGWDSREAIAGIRK
                     IIETVAVSAAVSNEQ"
BASE COUNT         1252 a         1292 c         1386 g         1320 t
ORIGIN      
        1 atcggccgag gcggcactaa cggtcaacga cacattggtc gagctgtggt ctgtcctggt
       61 tatttgcatg agcccctatt atataatttt tagcgctaag tgataagtta ggcttataat
      121 tcataaaata tggcaggagc ccgcaatcga ccgcgccaaa gctcgccgct taatctgccg
      181 agcgcttttg acctctttaa acccagtaaa gaaatcgtcc tcaagaatat ttggattttt
      241 ggtccgctgt acgctgtgcc ggtaattcta tgggtgcata attggatctg gacgccggct
      301 gcctccgggg gcagctggtg ggaaaagagt accggtgttt ccggcaactg gggattcagt
      361 ccgatagctt cgtggggtta cgccactgtc ggtttttcca tcatatggtt cctactagcc
      421 gccgtcggcg gcaccatcgc ccagtttatg ataaccgtcg ccgctttgca ggcttctgaa
      481 gataaagctc tggactttcg tgagttatgg caagtggtca aaaaaatcgg ctggcgacta
      541 ctgggattgt acttgttaat cgctctttac gtaattgtcg ggttcatttt gttgatcata
      601 ccaggcataa ttatgatccg ccgctatttc ctggcgccgt acgtgatgat tgataaaaat
      661 tgtggtgtta cagaggcgat ggagctcagt gccgccatga gcaagcctta ttccggcagc
      721 atttacggta tcatcggtgt tacgattttg atcggcctta ttggcatagt gccatttatc
      781 ggcagcctgg catcactgat tgtcggcatg ctttacgtgc tggccccggc cctgcgctac
      841 caacagctca agaaattaac ttaaacgttt gatggcggtg gcgaaggcgg cttccgtgag
      901 agacattttt tgcttttgtg aaagttcgta aaccgcatcg gtggctttga ctaagtgatt
      961 ttccagttcg cggttgactt tatcttccgg ccagtactct tcgttcttgt tttgcagcca
     1021 ttccaggtag ctgacaatca cgccgccggc gttggccaaa atatccggca cgattattac
     1081 gccgcggccg cttaaagatt tataggcttc gtagctgacc ggcccgttgg ccagctccaa
     1141 aatcattttg gccttaacgc cggcggcatt cttatccgtt acggcgtctt ccagcgccgc
     1201 cagcaccagc acgtccacat ccaactctat taattcgtcg ttacttatcg ctttaccggc
     1261 ttttaagtca accaagcggc cgccgctttt cttgaattca tcaaccgact tggctgacag
     1321 tccattccca tcgtaggcgc cgccgctgga atcactagcg gctactagcc gccatctcgg
     1381 atgctccttc tcggcaatca aggcgaaata tgaaccaacg ttaccccagc cctgcacagc
     1441 aaagctgacc gaaccggaaa tacttgattt gttaagtatt tcccgcagaa taatcacgcc
     1501 gccgcggcca gtagccgcgt cgcgccccag actgccgcct ttttccacgc tcttgcctgt
     1561 aaagccggct ttggtcttgt cgccgctgag ccgctcaaat tcatcaacca tccaatcaat
     1621 aaccttggca ttggtgccga cgtccggtgc cggcacgtct ttgtccggcc cgatatactg
     1681 gtgcagcccg cggacatatt tgcgcgaaag ctcttccaac tcagcctcgg tcaactcttt
     1741 ggggttgacc accaccccgc ctttaccgcc gcccatcggc aagccggcaa ccgcggtttt
     1801 aaaactcatc agtgtcgcca gtgcccggac ttcatcaaaa tccacattgg ggtgaaaacg
     1861 tataccaccc ttatacggcc cgagcttatt gctgtgctgc actctgtaag ctgggtactt
     1921 tttgcggttt gacagctcta tttcaaaatt atgttcggcg tcgggggcta tcaattgatc
     1981 aacttcatca tctggcaaac ctagtttttt gccgacgtct ttaattagag cagtggcggt
     2041 gtctagcatt aatcctgcac tctattataa accgtctagt aggtagcaac ggtggtaaag
     2101 tctacttgga ccgagtacga gccgaatggc gtggagatag aaggcgtggc agcaaacttt
     2161 agaatgaggg cttcgtcggc aacggtgttg ttggtggctg cgctggcaat ctgggttgag
     2221 gcgccacttt cctgccaagc aaaaccattg gtcgtgccgt atgagccggt ggcgccgaca
     2281 tagttagtgg agggcaccag atgctcgctg ccgctggaat atatacaggt gtagtaactg
     2341 ttagctgtca ctgttccgca gtttgtgccg ccgatggtca tgccaaagtc ttcggtacca
     2401 gcggtaaaag tggtttgggt cgttcccgcc gagttgatac attgatcagt attaacatta
     2461 ccggcattgc acgaggcccc ggtaatgcgc agtgcgccta aatggtttgt cccgcttgag
     2521 gcttggattg cgtcataact taccgaggag ccgttgatag cattggttcg taataccgcc
     2581 accccgttta cattgttgcc gccgccagca ttgaccggag agatattgat ggtggtgggg
     2641 tccagggtgc cgatattgac cgtggtgccg ctaaccgaac tgcagctacc cacggcggtg
     2701 gtcgcgtcgt caacagccgt agaaccgatg cagaattgta aaatttctgc taccgcagcg
     2761 tcaactgtaa gagtttgaac aaccgcactg gcgaccgtgc cgctgtctgt tggagagtta
     2821 aaagaagcat ctgaatatag atacataccg atgtagaaag tactgttagc tgtcgtagga
     2881 ttagtctggg tattccaggt taaggttttg ctggtggcgg tctcgtttgg gctggtggct
     2941 gtgcgtgtga tacatagcac gttgttgttg ccgttctcat cacagtcatt aagattggaa
     3001 ctttgtctgg taaagccggt ggtattttgc cagtttccgg caagagtacc aacctgggag
     3061 ccggcattaa tattcagccc ggtgggcgcc gtacaactac ccaaagtgct tccgctcagg
     3121 ctgtaggtac tggttgccgt ggtacaggcg acaaatttta ttgactgaat agtggtaacg
     3181 gttggctgtg taaaggtata agtataagac aagccggtcg cgcctggaac ccccgaagaa
     3241 atgcttagcg agcgtgggac aatttgaccg gcgaacgcat caccgggcac aagagcaggt
     3301 accagccata tagtggcaag agtcataacc aagacaaata atggaacctt gtacaggtgt
     3361 ttctgccaga ccctaaaggc tcgaaacgat ttcatcgaag atttttgttt ttgttaacct
     3421 gactatttta tatcacgcct tcgttgcaag ctcaagaggt ttataagaag ttatccacac
     3481 cctggtgggt gagagaggac ttgaacctcc acgccttgcg gcaccaggtc ctaaacctga
     3541 cgtgtatacc aatttcacca ctcaccctta aaagaggtat tttaccttat tttgcggtaa
     3601 aagaccagtt gggcgtcacc gtaatttttg tcggttattg tgggagtttc cgttaaagac
     3661 tttacagtta gtagtgaaat attatataat taccatatca acttgaggcc ggttatgacc
     3721 aacccatttg aggaaagatt aaatcgagtc accgaacgaa cccgtcagga gcagcttgat
     3781 aaagagcggg cgcttacaga ggcggcggag cggctcaggg tagaaagaga ggccagggcg
     3841 caaagagcaa gattgctggc cgaagaaatc tctcaagcca aggcgctgct gctgcgtttt
     3901 gatactccgc aactagagta ctctgggaac tacgaataca aagaaactca taccattccc
     3961 gcaacagact ggatacactg gcgctacaag ggtactaggg ttaccgagcc acacaagaaa
     4021 tcacgaggca gctggttcaa taaaggggca gagtgggctc aggaaacaag ctacacattt
     4081 agcggctggg aggttactgc taccggcaca gaagggaacg ccgaaccata ccacggggca
     4141 agaggcacgc tagttgttcc ttcggacaac tccccaatgc atttaagcat ccagggttta
     4201 aaaaataatc atttttcgtt agacgaggcg atatcaattg gtaaatttaa ttggtccgtc
     4261 gctgttaata gcggctacgg aggctggcgg ccgatagacc acgagttcag cgtagatagt
     4321 tttttagaaa actgcgccga aaaaatcgct gcttacttag cgaccaaaca aggcgtctca
     4381 tgagcgaatc tgaacccaca gacaggctgc cgctaacggt ggagcacatc aaagaccatc
     4441 tgctagaaaa gctatccggc attggcgacc tagatattgt gctattcggc tcaaatgcgc
     4501 ggggaaatca gattagcgcg gagagtgata tagatatatg gattagcggc gagcccgaaa
     4561 cagaaaacag agcttccgag ctgttgtctc aagattcgga gctgccagcg ccatatgatg
     4621 ttgttaggct cggtgattac gccggccggc cggaactgga atccgcaatg agatttgtag
     4681 tcggctctac aggtgttctc atagccggcg agctacctag ttaccccgaa gattttgacg
     4741 ccgaaaaagc ggcagcggtt ttttccgccg ccgccctcag acaagcggaa gaactaatag
     4801 accgcgccga cattattgaa cgttcaggcg ggtggtcaaa cgggtcattt agccgtgaag
     4861 cagcccgctc cctgctctac gcccaaagtg ctagaaccgg cgattgggcg agcgtaaaaa
     4921 aacttgatgc gcctgcgctc gcgaagaaat tggaagatgt cgacattgaa ctggggtcac
     4981 tgattcgcac tagcgggtgg gacagccggg aagctatcgc gggcattaga aagatcatag
     5041 aaactgtggc cgtgtcggca gccgtgagta atgagcagta gtttttgctc ctaggctttg
     5101 cggtaaaaga ccagctggga gtcgccgtag tttttgtcag ttataagctg gcaattgtca
     5161 aaagctggag gcagttcgtg cccgggccag gagaccacta tgataccgcc gcttttggcg
     5221 tgtttggtga gtttacccag caattttagc
//
LOCUS       DASPTF010000057         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1826676, whole genome shotgun sequence.
ACCESSION   DASPTF010000057 DASPTF010000000
VERSION     DASPTF010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826676"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VEH48_01830"
     CDS             <1..242
                     /locus_tag="VEH48_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXY18141.1"
                     /translation="YLVSDGSGSPAIVAGSPADKAGLKEGDIITKVNSTAIDNTHSLT
                     GVLAQFNPGDQVTLTVIRGGKTMNIQATLGTAPTG"
     gene            367..1308
                     /locus_tag="VEH48_01835"
     CDS             367..1308
                     /locus_tag="VEH48_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18142.1"
                     /translation="MPGEEELDIGQNAESEHFRAEHQERWEDTADALNKARPGFKKTL
                     GLGKVTAEGVMRDEAAADDKAMTELMQEQPRNRPGSPYVEIDLSDPDIARKIQEFRDK
                     LQGELTTMKSIELRREAKGENRYRAPELNPFLRTTSQEFALQMLNILQRDGKLNFWDM
                     SVDLKKKDPTTPTFDRTTIIAFGTAAEWISQELGIEEKKVPESGAETPEAKVAQSPEL
                     SHEQAETPKAGYTLEYDVEDLKGGSLGSRSQLLPENLEEAQDTALKSVIDAEMNPATA
                     NQLVRNAKVKMPDGTVVPWQEFTDPANQRAREKTEAA"
     gene            complement(1340..>2111)
                     /locus_tag="VEH48_01840"
     CDS             complement(1340..>2111)
                     /locus_tag="VEH48_01840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HXY18143.1"
                     /translation="AGIGTARLDVLVLLEDCLRTSRAHILAHPETPIQGLPLKRLSAK
                     LARRARHEPLAYIRGFSEFYGRQFKIDRRVLQPRPESETMIELLKTLRLPINPRIADV
                     GAGSGALGITAMLEVPRAVTDLFEIDENALKVAELNVKKYRVSARCVFSDLLRASRDA
                     YDVILANLPYVPSYHHVNEAAGHEPRLAIYGGGDGLEIYRRLFNQLAEHYSPPQFVLT
                     ESLPNQHARLADIAAGAGYRETARRDFIQVFEIPKILD"
BASE COUNT          584 a          529 c          580 g          418 t
ORIGIN      
        1 cgtacctggt gtctgacggc agcggcagtc cggccatcgt agccggcagt ccggccgaca
       61 aggccggcct gaaagaaggc gatatcatca ccaaagtcaa cagtaccgcc atagataaca
      121 cgcacagtct taccggcgtg ctggcgcagt tcaatcccgg cgaccaagtc acgctgacgg
      181 tcatccgagg cggcaaaacc atgaacatcc aagctacgct tggtacagcg cccactggtt
      241 agttgtcgca ggtaattgca gatactcact tttagaacta acggagcagg actctagcta
      301 ttgacataac agatgtatgg atttacgctt aacccgtaaa cttaaaaaat aaataaggaa
      361 ataaaaatgc caggggaaga agagttagat atcggccaaa atgctgagag cgaacatttc
      421 agagccgagc accaagaacg ttgggaagac acggctgatg cactaaacaa ggcaagacct
      481 ggcttcaaga aaaccttggg gctaggtaag gtcacagccg aaggtgtcat gagggatgag
      541 gccgcagcag acgataaagc tatgacggaa ctcatgcaag agcagccgcg taaccgtcca
      601 gggagcccat atgttgaaat tgacctcagc gaccccgata tagctcgcaa aattcaggaa
      661 ttcagggata aactacaggg cgaattgacg accatgaaat ctattgaact tcgccgcgaa
      721 gcgaaggggg agaataggta tagagctcct gagcttaatc ctttcttgcg tactacttcc
      781 caggaatttg cccttcagat gctgaatatc ctccagcgag acggaaaact taatttctgg
      841 gatatgagtg ttgatcttaa gaagaaagat ccaactactc caacatttga taggactacc
      901 atcatagctt ttggcactgc ggctgaatgg ataagccagg aacttgggat agaagagaag
      961 aaagttcctg agtccggagc tgagactccc gaggcaaaag tagcccaatc tcccgagctt
     1021 tcccacgaac aagcggagac accaaaagct ggatatactc tagaatatga tgtggaagat
     1081 ctgaagggtg ggagtttagg ttcccgatca cagctgcttc cggaaaatct agaagaagcg
     1141 caggacaccg ctttaaaatc agtaattgat gctgagatga atccagcgac cgccaaccaa
     1201 ctggtgcgga atgccaaagt taagatgccg gacggaacag ttgtaccatg gcaggagttt
     1261 accgatcccg ccaaccaaag agctcgcgaa aaaactgaag ccgcgtaaat cccgataagc
     1321 gtaaaaacaa aatacttgac tagtcaagta tttttggaat ttcaaagact tggatgaaat
     1381 cgcggcgagc ggtttcacgg tagccggcac cggcggcgat gtcggccaga cgagcgtgct
     1441 gattaggcag agattcggtg agtacgaatt gtggcggtga atagtgttcg gccagctggt
     1501 taaatagccg gcggtagatt tccaggccat cgccgccgcc gtaaatcgcc agacgcggct
     1561 cgtggccggc ggcctcgttc acatggtgat agctgggcac gtagggcagg ttggctaaaa
     1621 tgacgtcgta agcgtcgcgg ctggcacgca gtagatcgct gaaaacacag cgggcactga
     1681 ctcggtattt cttaacattc aattcggcga cctttagcgc attttcatca atctcgaaca
     1741 ggtcggtgac ggcccgcggc acttccagca tggcggtaat acccagtgcg ccgctgccgg
     1801 ctccgacgtc agcgatccgc gggttgatag gcaggcgcag ggtctttaga agctcaatca
     1861 tggtttcgga ttcgggacgc ggctgcagca cgcgcctgtc aatcttgaac tggcggccgt
     1921 aaaactcgga gaagccgcgg atgtaagcca gcggctcgtg ccgcgcccga cgtgccagct
     1981 tagcgctcaa cctcttcaag ggcaaccctt gtataggggt ttccgggtgc gccaaaatat
     2041 gcgcgcggct ggtacgtagg caatcttcca gcaaaaccaa cacgtccagc cgcgccgtac
     2101 caatcccggc a
//
LOCUS       DASPTF010000058         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_521180, whole genome shotgun sequence.
ACCESSION   DASPTF010000058 DASPTF010000000
VERSION     DASPTF010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521180"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VEH48_01845"
     CDS             complement(<1..478)
                     /locus_tag="VEH48_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18144.1"
                     /translation="MDKPTLEDIVSLAKRRGFIYPSSEIYGGLAGVYDYGPLGVMLKH
                     NLENLWWSSFVSSWDDMYPMDAAILMNQKVWEASGHAGGFNDPFVECSNCHSRIREDH
                     LKERQEKGGLRVSRTIEKCPVCGKAGTLSEPKQFNMMFKTQIGTQASEEDASYLRPE"
     gene            complement(499..1260)
                     /locus_tag="VEH48_01850"
     CDS             complement(499..1260)
                     /locus_tag="VEH48_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HXY18145.1"
                     /translation="MEEKLEFAQGLARKAGQIILDYFKADQKISYKTDNTPVTVADKL
                     INSHVIERIAQTYPDHGVVGEEESTERTDQENVWICDPIDGTKAFTWGLPTAMFSLAL
                     VTEGRPVLGAAYDPFLDRMFTGIHGQGAFCNGEQLSVSKDNLQTGIMAATAEIEKFRT
                     ERYKYILDRGIRLAVFSGFVYKMCLVAEGRLVGHTEDNVGRHDIAAMDVIIQEAGGRV
                     TDFADKPLDYSRPFKGTVVSNGVVHDELLKIAQIK"
     gene            1397..1567
                     /locus_tag="VEH48_01855"
     CDS             1397..1567
                     /locus_tag="VEH48_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18146.1"
                     /translation="MRRQMNEELKQLELKNAEWYAQNPNASWFRRALHAFVHWLDRGG
                     TVNKYNFNDNGW"
     gene            1669..>2171
                     /locus_tag="VEH48_01860"
     CDS             1669..>2171
                     /locus_tag="VEH48_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18147.1"
                     /translation="MAAPGESPVYDTQDSTLILLNDLQSESISRRYEFEDHFRLGIQL
                     AKFYVASGFVPTFRHAAESHYKAAMQKAKRKPLEKARIHIFASHLKISDPAATEQDLI
                     AAYSALDEARQLALRKTPRAKQRALRDQLLVNVAALMQYLESRGIKPEQARQESSESV
                     GQVATGGV"
BASE COUNT          537 a          564 c          528 g          542 t
ORIGIN      
        1 tctccggccg caagtaactg gcatcttcct cgctggcctg cgtgccaatc tgggttttga
       61 acatcatgtt gaactgcttg ggctcgctta gggtgccggc tttaccacaa acaggacact
      121 tttcgatagt tcgagatact ctcaatccac ccttttcttg tctctctttg agatgatctt
      181 cacgaatacg cgagtggcag ttgctacact cgacaaaagg gtcgttaaag ccgccggcgt
      241 ggccggaagc ttcccagact ttttgattca tcaaaatggc ggcgtccatg gggtacatat
      301 cgtcccagga gctgacaaaa ctgctccacc acaagttttc caggttgtgt ttgagcatca
      361 cgcccagcgg gccgtaatcg taaacgccgg ccagcccgcc gtaaatctcg ctgctggggt
      421 aaataaaccc ccttcttttt gccagcgaga caatatcctc aagagtaggc ttatccatta
      481 gctaaaatta taccgttatt attttatttg ggctattttt agcagctcgt catgtacaac
      541 gccattagag acaaccgtgc ctttaaatgg ccgggaataa tctaagggtt tatcggcaaa
      601 atctgtcacc ctaccgccgg cctcttggat aataacatcc atagccgcaa tgtcatggcg
      661 accaacatta tcctcggtgt ggcctaccag ccgcccttcg gctaccaaac acatcttata
      721 aacaaaaccg ctgaacaccg ctagtctaat gcctcggtcc aaaatgtatt tatatcgttc
      781 tgtcctaaat ttttcaattt cggctgtagc ggccataatg ccagtctgga gattatcttt
      841 agaaacggaa agctgctcgc cattacagaa agcgccctgg ccgtggatgc cagtgaacat
      901 acgatctaaa aacgggtcgt aagcggcgcc cagcaccggc cggccttcgg taaccaaggc
      961 caaagaaaac atagccgtcg gcaggcccca agtgaaagct ttggtgccat ctatggggtc
     1021 acatatccaa acattttctt ggtctgttct ctcggtactc tcctcttcac caacaacacc
     1081 atgatccgga taagtttggg cgattctttc tataacgtga ctattgatta atttgtcggc
     1141 taccgttact ggagtgttat cggtcttgta agagatcttt tgatcggcct taaaatagtc
     1201 aaggataatc tgtccggctt ttcttgccag gccttgggca aactccagct tttcttccat
     1261 cttaactaca gattatatcc gaacgtgtgg cctaatcata gattagaaat cgctattgca
     1321 taagcgtaag gtttattgta atatatcggc tatccgacac cgcgagggag gagttatggt
     1381 ggagctgccg agcgaaatgc gcaggcagat gaacgaagag ctcaagcaac tcgagctcaa
     1441 gaacgccgag tggtacgcgc agaatcccaa tgcctcctgg ttccggcgag cattgcacgc
     1501 ctttgtgcac tggctcgacc gaggaggcac tgtcaacaag tataacttca acgacaacgg
     1561 ctggtgatcg gacttccggg tcgccgggga ggtgcgcggg ctgtcagccc cgccttcccg
     1621 gccccgggtt cttttttatt aaaggactgc tgtatacttg ggaatattgt ggccgcgcct
     1681 ggagaatctc ccgtttatga cactcaagat tcgacgctga ttctgttaaa cgacttgcaa
     1741 tcggaaagta ttagccgccg gtacgagttt gaggaccatt tccggctcgg tatccaactt
     1801 gcaaaattct atgttgccag cggctttgtg ccgacattcc gtcatgcggc agagtcgcat
     1861 tacaaagctg ctatgcagaa agctaagcgg aagcccctgg aaaaagcgcg aatccatatt
     1921 tttgccagcc acctgaagat atcagacccg gcggctaccg aacaggattt aattgcagct
     1981 tacagcgctc tggatgaagc ccgccagctg gcattgagaa agacgccgcg agccaagcag
     2041 cgcgcgctta gggatcagtt actagtcaac gtcgcggcac ttatgcaata tcttgagagc
     2101 cgaggaatta aaccggagca agcaagacag gaatcgagcg aatcggttgg acaagttgcg
     2161 actggcggcg t
//
LOCUS       DASPTF010000059         7049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1682955, whole genome shotgun sequence.
ACCESSION   DASPTF010000059 DASPTF010000000
VERSION     DASPTF010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7049)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682955"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..362)
                     /locus_tag="VEH48_01865"
     CDS             complement(174..362)
                     /locus_tag="VEH48_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18148.1"
                     /translation="MVKKWKDNKKMGGHPGGPVYGLGIIGSAVYYLHTTTGFWPSVVA
                     LLKALVWPAFLVYHLLAG"
     gene            429..1667
                     /locus_tag="VEH48_01870"
     CDS             429..1667
                     /locus_tag="VEH48_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18149.1"
                     /translation="MRYKSAGSQSGFSHFLLLLAVIAVVGAVGYAGWRVHNSSAGGQQ
                     GSKILVSHSSAEAKAVAAGKMLSNNKCQGTGKLMFTHLPMNQGDFGFLVPYGDVIGGH
                     VTPIDHQYFTPASYNSARDSYPVYAMADANIVDIQPRTTPGHGTDYRLIFAHSCTSLY
                     YYDLLTDLSGAAKVAYDKGGDINLPVKAGDRVGLIGGQTLDFALWDTSSHLTGFVNPS
                     SYDGEAWKIYVADPFPSYTPDLRAIMVARDPRTAQPIAGKIDYDIDGKLIGNWFLAGS
                     GGYHSPSNTAQDYWTGHLSIAPDVYDPSVFVISLGDFGGQALQFIATGNTPDPATVGV
                     NTGLVKYDLNKYGYDKGDGSRWDNMTMTLNPEPVAISGDEGCLLAQLTATRSLKAESF
                     PGTACSGISGFDSSAKTYTR"
     gene            complement(1689..2117)
                     /locus_tag="VEH48_01875"
     CDS             complement(1689..2117)
                     /locus_tag="VEH48_01875"
                     /inference="COORDINATES: protein motif:HMM:NF020947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HXY18150.1"
                     /translation="MDKKQDFGQKAVIVVNKELEQWEVLNTVAHISAYIGHKLEDKFG
                     TGENFVTKDGIDHPRNSQYAIIVLRAKPGQLPNLMAKVRESGLLYHGFIREMIDTTDD
                     AEITKILTKKNDDEIEYLGIGVFGPKAEVGSLTKNYQLWR"
     gene            2205..2651
                     /locus_tag="VEH48_01880"
     CDS             2205..2651
                     /locus_tag="VEH48_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18151.1"
                     /translation="MPGMMPGLPEAVTYDEYEELRDSGLPPSSIHVARPYTLRWRYSQ
                     PTRECFAHDLLSLIHEIKHLGRYSLVGARVRDFDRVKKVDLGISNSGIVFHLSPLPED
                     LDSIPYQSEIGYFVGEAGSSNSNEISDRKSEEVEVDEWLGSLALSR"
     gene            2700..3203
                     /locus_tag="VEH48_01885"
     CDS             2700..3203
                     /locus_tag="VEH48_01885"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXY18152.1"
                     /translation="MTVSHDSETPAHGQQVFSVCAFVHQKFEGVEKLFLPRRAKTKKF
                     WPDVYELPGGHVDWGETFVDALIREVKEEFGMTINVGDPFYVFTYDNPVKGSHSVEIV
                     YFATFIDPLDKITFDKDDHSEYKWLSEDELPQIYGIGGKNEDNPEVLAMKRGFELLNG
                     HGYIDFG"
     gene            complement(3222..3803)
                     /locus_tag="VEH48_01890"
     CDS             complement(3222..3803)
                     /locus_tag="VEH48_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXY18153.1"
                     /translation="MKAYLIPGAFEDVKSRGYRALLDTYKELGYQPEFVRIDWKYKTI
                     DDWVEQVKSEIPKENVQSSLLSGFSWGSMIALAVAAEHTNPKKLLLFSLSPYFAEDLP
                     HVKKPWFRWAGKNRISTFKNFYMNELADKIDCPTVIFIGSKEVSKYSDMRRRTREAHK
                     RIKGSRLVIIDGVKHDVADPKYVAAIKETLKNT"
     gene            complement(3915..4667)
                     /locus_tag="VEH48_01895"
     CDS             complement(3915..4667)
                     /locus_tag="VEH48_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18154.1"
                     /translation="MSVSQEIKSGYAAIAKASSDSDRVKAYAEFHRQLDGAKISVDDI
                     EGASRQSGTIFEATKAARDKKVRIKRGTKRLLRGSGIIVVGSYDGDILAADYHRDLEN
                     EDLVANKYPGYQKIAVRKIGQALLLGSVNAKSVVNQYHRLQEMLVESGLTETSAPPHH
                     LGYAGGHGLTVGASGMLPTDEVAAELSELLAPELVEGYRRRWVDWKHDEFAGAIDLAA
                     ADHVIHPTDAIKTIHELTAYIRSYHDHGLNPH"
     gene            4771..4962
                     /locus_tag="VEH48_01900"
     CDS             4771..4962
                     /locus_tag="VEH48_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18155.1"
                     /translation="MDIPKARDLYNGDINEAVVAVQNATGVKIDPEWWAANVGNEGTP
                     EEILDRFDKAYAEFVAAKK"
     gene            complement(5012..5389)
                     /locus_tag="VEH48_01905"
     CDS             complement(5012..5389)
                     /locus_tag="VEH48_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXY18156.1"
                     /translation="MKSIAGIVCYVKDTAKTAEFYERLGFTFTKREPDHISIRLNWFW
                     IDFHPQDKEDKPEFQKEANLDNKGAGQFLYISVDDVDGFYKGLLVKGLKPSSEPRDWP
                     WGNREFVIRDPDGYKLVFFTRLK"
     gene            complement(5398..6711)
                     /gene="aspS"
                     /locus_tag="VEH48_01910"
     CDS             complement(5398..6711)
                     /gene="aspS"
                     /locus_tag="VEH48_01910"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HXY18157.1"
                     /translation="MERVLSSEVAKHAGQAVKVQGWLHKKRLIGGINFIVLRDRGGLV
                     QIVLQDENEVKKLHGLHVGTVMSIEGQAVEEPRAPGGAEIHDPKITVDVPVADEPPIE
                     IDKPLSHKPENLDTLFDIRVLGLRSLQEQAIFKIQAAVEESIRKFLAENDFTQFNSPK
                     LLPGATEGGAEVFKLDYFGKEATLAQSAQFYKEIMVGVFERVFEINPTYRAEPSATTR
                     HMTEFITIDVEMGFINFQELLDLLSNLINAVVNDVWQKCEAELKLWGAKKPTLAEHFP
                     AITMAEIHKLYSQKTGEDTTKELDLIPSEERWICEYAAKEFGSEAVFVTEWPQADAKF
                     YHKLIDDKPELAERFDLLFRGVEIATGSLREHRYDKLVTQLKEKTGGTPDDPGFHYFL
                     QAFKYGMPPLGGFGMGLERLVEKIIGLSNVKEATLFPRDINRLSP"
     gene            complement(6730..>7049)
                     /locus_tag="VEH48_01915"
     CDS             complement(6730..>7049)
                     /locus_tag="VEH48_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXY18158.1"
                     /translation="LDVVGSLYLPDLLENLRRSGHVRDIMQPGAGYIDEIASLHDCLE
                     QFLKTGRQLLVVTNSFEETAGVITLEDVLAQITGRKFTNKLAEPAGLEADKAAKTPDQ
                     TDK"
BASE COUNT         1655 a         1816 c         1711 g         1867 t
ORIGIN      
        1 tagagctatt tgacgaatta tgaaagatgg cccaagcccc gccggcgata atcaccagca
       61 cgaccaggcc gactaacatt ttggcgttcc tctgcatcgc aaacctccta aattagtcct
      121 tccattgtag caagacacta gttcggcaga aaatacttaa caagttaagt attttagccg
      181 gctaacaggt gatagaccaa gaaagctggc cagaccaggg ccttcagcag agccaccacg
      241 ctgggccaaa agccggtggt ggtatgcagg taataaaccg ccgaaccgat tatgcccaag
      301 ccgtaaaccg ggccgccggg gtgtccgccc atcttcttat tatctttcca ttttttaacc
      361 atgattgcct ccttctccca ttgtagcgcc aaaatccgga atccatacct ataatgtaaa
      421 ataagcacat gcgttataaa tcagccggct ctcagtccgg tttcagccat tttctattgt
      481 tgctggcggt catcgccgtg gttggagcgg ttggctacgc tggttggcgg gtccataaca
      541 gtagcgccgg cggccagcag ggctccaaaa tcctagtcag ccacagctcg gccgaggcca
      601 aagccgtggc tgccggcaag atgttgagta acaacaagtg ccaaggtacg ggcaagttaa
      661 tgttcaccca cctgcccatg aaccaaggcg actttggctt cttagtccct tacggcgatg
      721 tcataggcgg acatgtcacg ccaattgacc accaatactt cacaccggcc agttacaatt
      781 cagcccgcga cagctacccg gtttacgcca tggccgatgc caacatagtt gacatccagc
      841 cgcgcaccac gccgggccac ggcactgact accggttgat attcgcccac agctgtacct
      901 cgctttatta ctacgacctg ctgactgacc tgagcggagc ggccaaggtc gcctatgaca
      961 agggcggcga catcaacctg ccggtcaagg ccggtgaccg agtgggtctg attggcggcc
     1021 agacgctgga cttcgccctg tgggatactt ccagccactt gactggcttc gtgaacccgt
     1081 ccagctacga tggcgaggct tggaaaatat acgttgcgga tcccttcccc agctacacac
     1141 cggatttgcg tgccatcatg gtggcccgcg acccgcgcac cgcccagccg attgccggaa
     1201 aaatcgatta cgacatcgac ggcaagctca tcggcaactg gtttctggcc ggcagcggcg
     1261 gctatcacag ccccagcaac accgcccagg actactggac cgggcatctg tctatcgcac
     1321 cagacgttta tgacccctcg gtgtttgtaa tctcgctggg cgacttcggc ggccaggcat
     1381 tacaatttat cgccaccggt aacacaccgg acccggccac cgtaggagtt aacacaggtt
     1441 tggtgaaata cgacctgaac aagtacggat acgacaaggg cgacggctcg cgctgggata
     1501 acatgaccat gaccctcaac cccgaaccgg tggccatcag cggtgatgaa ggctgcctgc
     1561 tggctcaact gacggcaacc cgtagcttaa aggccgagtc attccccggt acagcctgca
     1621 gcggtatcag cggctttgac tcttccgcca aaacctacac gagataaaaa tacttaacaa
     1681 gtcaagtatt atcgccagag ttggtagttc ttagtcagag agcctacttc tgctttcggg
     1741 ccaaaaacgc caattcccag atattcaatt tcgtcatcgt ttttcttggt caagattttg
     1801 gtgatttcgg cgtcgtcggt cgtgtctatc atttcgcgga tgaacccgtg gtaaagcaag
     1861 ccggattcgc ggacttttgc cataagattc ggcaactggc ccggtttggc ccgcagaaca
     1921 ataatggcat actgtgaatt gcgcgggtgg tctatcccgt ctttggtcac aaaattttcg
     1981 ccggtgccaa atttatcttc cagcttatgg ccaatatacg cggaaatatg ggcgacggta
     2041 ttcaatacct cccactgttc cagctctttg tttacgacta taaccgcctt ctgcccaaaa
     2101 tcctgttttt tatccatctg tttattttat ataccaagcc ctggagttca tactttttcc
     2161 ataagaattt tttatctaag catggttgtg gcataattct gcaaatgcca ggaatgatgc
     2221 ccggtttacc cgaggctgta acctatgatg agtacgagga attgcgcgac agcggtctgc
     2281 cgccttcttc tatacatgtg gctaggcctt atacgcttag gtggagatat tcccagccaa
     2341 cccgcgagtg cttcgcccac gatttactca gtttgattca tgaaattaag caccttggaa
     2401 gatatagcct ggtaggcgcc agggtccgtg acttcgatag agtgaaaaaa gttgatttgg
     2461 gtatcagcaa tagtgggatt gtttttcatc tctcaccgct acctgaggat ttagattcta
     2521 tcccgtatca gtctgagatt ggctatttcg ttggtgaagc tggttcatcg aattctaacg
     2581 agatctccga ccgaaagtca gaagaagttg aagtggatga gtggcttggc agtttggcct
     2641 tatctcgtta gcctagcgcc aaaccccggg ctttgaccct gaagcgtata atcaatttga
     2701 tgacagtctc gcatgacagc gaaacgccgg cgcacggcca acaggttttc agtgtctgtg
     2761 ccttcgtgca ccaaaaattt gagggcgtgg aaaagctttt cttgccacga cgggccaaaa
     2821 ccaagaaatt ttggccggat gtttacgagc tgcccggcgg ccacgtggat tggggcgaga
     2881 ctttcgtgga tgccctgatc cgcgaagtca aagaagaatt cgggatgacc atcaatgttg
     2941 gcgacccgtt ttatgttttt acttacgata atccagttaa aggctcccat tccgtggaga
     3001 ttgtctattt cgccactttt atcgacccgc tagataaaat cacttttgac aaagacgacc
     3061 actctgaata caaatggctg tccgaagacg agctgccgca aatctacggt atcggcggaa
     3121 aaaacgagga caacccggaa gtattagcca tgaaacgcgg ttttgaacta ttaaatggcc
     3181 acggctacat agacttcggc taaagaaaaa tacttgactt gttaagtatt ttttagggtc
     3241 tcttttatcg cggctacata tttggggtcg gcgacatcgt gcttcacccc gtcaataatg
     3301 actagtcggc tacccttaat ccgcttgtgc gcctcgcgtg tgcgccgtct catatcacta
     3361 tatttactga cttctttact accgataaaa attaccgttg ggcaatcaat tttgtctgcc
     3421 agttcattca tataaaaatt cttaaaggtg gatattcggt tcttgcccgc ccatctaaac
     3481 caaggttttt tgacatgtgg caggtcttcg gcaaaatacg gtgatagaga aaaaagcaaa
     3541 agcttttttg ggttggtgtg ctctgccgcg acagccagcg ctatcatcga accccaagag
     3601 aaaccgctca acagcgaact ttgcacgttt tctttaggga tttcagactt gacttgctcc
     3661 acccagtcat caatggtttt atatttccaa tcaattcgga caaattccgg ttgatagcca
     3721 agttctttgt aagtatccag cagcgctcga taacctcgtg attttacatc ttcgaatgca
     3781 cccggaatca aataggcttt catgggttta attgtagcgc caaaccccgt aatttaggtt
     3841 cttgagctag cgattgaaac tagatactga gagattttaa cctctttcgg atcttaaaat
     3901 gtattattca aaggttaatg agggttaagt ccatggtcat gatagctacg gatataggca
     3961 gttagttcat gtattgtctt tatggcatct gttggatgaa tcacatgatc ggccgcagct
     4021 aaatcgatgg cgccggcaaa ttcatcatgc ttccaatcta cccagcgacg cctatacccc
     4081 tcaactagtt ctggcgccag taactcactt aattcagccg cgacttcgtc ggttggtagc
     4141 atcccgctag ctccgacagt taacccgtga ccgcctgcat aacccagatg gtgcggtgga
     4201 gcacttgtct ctgtaaggcc ggattcaact agcatttctt gaagtctgtg atactggtta
     4261 acaacacttt ttgcgttaac gctgccaagc aaaagggcct gtcctatttt tctaacggca
     4321 attttttgat aacccgggta tttgttggct accaggtctt cattttccag atccctatgg
     4381 taatcggcag ccaatatgtc accgtcgtag ctccccacaa caatgatgcc actgccgcgg
     4441 agaagtcttt tcgtaccacg tttaatcctt acttttttat cacgggctgc tttagtagcc
     4501 tcaaagatag tgccgctttg tcggctggcg ccttcaatat catcaacgga tatttttgcc
     4561 ccatccaact gccgatggaa ctcggcatag gcctttactc tgtcgctatc gcttgaagct
     4621 ttagcaatag ccgcgtagcc agactttatc tcctgcgaaa cactcatatg gaatattata
     4681 ctatatttta tgttttattc aataagtaca cggtcattaa atcttaatta agcgttcgga
     4741 gcttaagttt ttgagcgtat aatttgactt atggacattc ctaaagcccg tgacttgtac
     4801 aatggtgaca tcaacgaagc ggttgtagcc gttcaaaatg ccaccggcgt aaaaatagat
     4861 ccggaatggt gggcagccaa tgttggcaac gaaggaactc cagaagagat tctggaccgt
     4921 ttcgacaaag cttatgcgga atttgtcgcc gccaaaaagt agcgccaaac cccggaattt
     4981 aggttctaaa aatacttgac ttgttaggta tttatttaag tctagtgaaa aagactagtt
     5041 tgtagccgtc ggggtcgcga atgacaaatt cccgattgcc ccaaggccaa tcgcgaggct
     5101 cgcttgaagg ctttagaccc tttacaagca accctttata aaatccgtca acatcatcta
     5161 cgctaatata tagaaattgg ccggcgcctt tattatcaag attggcctct ttttggaatt
     5221 ccggtttgtc ttctttgtct tgaggatgga agtctatcca aaaccaattc aaacgtatcg
     5281 atatatggtc cggctctcgt ttggtgaacg taaagcctaa tctttcgtaa aactccgccg
     5341 tcttagctgt gtctttaacg taacaaacaa tcccggcaat gcttttcatc ttcatagtta
     5401 tgggctcaac ctattaatat cacgggggaa gagggtggct tctttgacgt tggataggcc
     5461 gatgattttt tctaccaggc gctccaggcc catgccaaag ccgccgagcg gcggcatgcc
     5521 gtatttgaag gcctgcagaa agtagtggaa gcccggatcg tccggtgtgc cgccggtttt
     5581 ttctttgagc tgagtgacta atttgtcgta gcggtgctcg cgcaggctgc cggtggcgat
     5641 ttctacgccg cggaaaagta aatcaaagcg ctcggccagc tctggcttat catcaatcag
     5701 cttgtggtaa aacttggcgt ctgcctgggg ccattcggtg acaaacaccg cctctgagcc
     5761 gaactctttg gccgcgtact cgcatatcca gcgttcctcg gacgggatta aatccagttc
     5821 tttggtcgtg tcttcgccgg ttttttggct gtaaagtttg tggatctcgg ccatcgttat
     5881 agccgggaag tgctcagcca gcgtgggctt tttggcgccc cagagcttaa gctcggcttc
     5941 gcatttttgc cagacatcgt tcacaacggc gtttatcaaa ttacttaaca agtcaagtaa
     6001 ttcctgaaag ttaataaagc ccatctccac gtcaatcgtg atgaactcgg tcatgtggcg
     6061 agtggtggcc gacggctcgg cgcggtaagt cgggttgatt tcgaacaccc tttcaaacac
     6121 cccgaccatg atttccttat agaactgtgc gctttgggcc agcgtggctt ctttgccaaa
     6181 atagtcaagc ttgaagacct ctgccccgcc ctcggtggcg ccgggcaaca acttgggcga
     6241 attaaactgc gtaaaatcgt tttcggccaa aaacttgcgg atggattcct caaccgccgc
     6301 ctggattttg aaaatcgcct gctcttgcag gctgcgcagg ccaagcacgc ggatatcaaa
     6361 taaggtgtcc aggttttccg gtttgtggct gagcggtttg tcgatttcta tcggcggttc
     6421 gtccgcaacc ggcacgtcta cggtgatttt gggatcgtgg atttcggcgc cgccgggcgc
     6481 gcgcggctcc tcaactgcct gcccttctat gctcatcacg gtgccgacgt gcaggccgtg
     6541 cagttttttt acttcatttt cgtcctgcag gactatctgg accaggccgc cgcggtcgcg
     6601 cagcacaatg aagttgatac caccgatgag gcgttttttg tgcagccagc cttgcacctt
     6661 gaccgcttgg ccggcgtgct tagcaacctc gctggataaa actcgttcca tttgcggtta
     6721 ttttaacact tatttatctg tttggtcagg ggttttggcg gctttgtcgg cttccaggcc
     6781 ggccggttcg gcaagtttat tggtaaattt acggccggtg atttgggcca ggacatcttc
     6841 cagcgtgatg acgccggccg tctcttcaaa gctgttggtg actaccaaca gctggcggcc
     6901 ggtttttaaa aactgctcca ggcaatcgtg caggctggcg atctcgtcta tgtagccggc
     6961 gccgggctgc atgatgtcgc gcacgtggcc agagcggcgc agattttcca gcaggtcagg
     7021 cagatacagg ctgccgacga cgtccagct
//
LOCUS       DASPTF010000060         3110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_818512, whole genome shotgun sequence.
ACCESSION   DASPTF010000060 DASPTF010000000
VERSION     DASPTF010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818512"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..682)
                     /locus_tag="VEH48_01920"
     CDS             complement(83..682)
                     /locus_tag="VEH48_01920"
                     /inference="COORDINATES: protein motif:HMM:NF001368.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HXY18159.1"
                     /translation="MDKKSQVLVPTVIEQEGRYERAYDIYSRLLRDRVIFLGDEVNEH
                     TANLVVAQMLFLENQDPTKDIIFYINSPGGSVYHALGIYDTMKYVKSDIQTVGIGVNA
                     SAAAILLSSGTKGKRLLLPHATVMIHQPSSAAQRAKISDLEIDLKEGLRLKRLLNEIL
                     AKNTGQPVSKIEKDVDRDYWMTADEAKKYGLVDKVITHR"
     gene            complement(727..1545)
                     /locus_tag="VEH48_01925"
     CDS             complement(727..1545)
                     /locus_tag="VEH48_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18160.1"
                     /translation="MFDKHKERSLLKIAREQEAERLLAEQEYARYSATESHREERESE
                     YIQDVLNVLGRAFFLLQFGVDPAIVGNARLEEKYKYEELTTPVLSIEYANLKFNPGKY
                     GVTNIRDPISAKYLRSPFGGRQYRRNFSRAEREIVERYAPNVFQPSFEAVCLCSGCQD
                     WGELGWYKKQYGTLQILKPVIADYGQVAQNLELFNEGSSGLALDCGTLPVFGLDPTNV
                     RAAIGCPTLIESIKAENECRKPRAVKSSGKLATGSIVSAARGLRLRREGKRPLN"
     gene            complement(1625..2920)
                     /gene="tig"
                     /locus_tag="VEH48_01930"
     CDS             complement(1625..2920)
                     /gene="tig"
                     /locus_tag="VEH48_01930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXY18161.1"
                     /translation="MQITRTDNSSTNVSLSVSAAGAELEPIKRHVLGRFAGQVKVPGF
                     RAGHAPASVVEKHIDQRALLDEFLNHALNELYRRAVDHQKLRPVGQPKIEVKKFVPFT
                     DLEFSAETDVIGPIILPDYKKIKLAKPKVEISAQDVNEVLKSLRERMAERKPVERPAK
                     NGDEILIDFSGKDSDGQPVAGADGKDYPLILGSNSFIPGFEDNLIGVKAGETKQFTVK
                     FPKNYGVAALQNKDVTFEVDAKKVSELAQPKLDDALAAKAGPFKTLAELKADIKKQLK
                     IERQNQADRDYENQLVQTIADKTTVDIPESLLTEQVLAMEEEEKRNLTYRGQTWQEHL
                     KEEGISEQQHRERNKPVAAGRVKAGLALSEIAEQEGLKVTPEELEIRLQLLKGQYQDP
                     QMRAELDKPENRNDIANRLLTEKTLEKLTDYTTKQRVPK"
BASE COUNT          678 a          811 c          747 g          874 t
ORIGIN      
        1 gtacactact tcttaggtta tagaatatag aataaagagt taagaatcaa gggttcttag
       61 aaaaaagtgt tgtagaattg tactatctgt gcgtaatgac cttgtcgact aagccatact
      121 tcttggcctc gtcagcggtc atccagtagt cgcggtccac atctttttca attttagaaa
      181 ccggctggcc ggtgttcttg gccagaatct cattaagcag acgcttcagg cgtagacctt
      241 ctttgaggtc aatttccaga tcggaaatct tggcccgctg agcggcgctg gacggctggt
      301 gaatcattac tgtagcgtgc ggcaaaagta agcgcttgcc cttggtgccg gaactgagca
      361 aaatggccgc ggcgctggcg ttaactccta tgcccacggt ctggatgtcg gacttgacgt
      421 acttcatagt gtcgtaaatg ccaagggcgt ggtagacact gccgcctggg ctattgatat
      481 aaaagatgat gtctttggtc gggtcctggt tctctaaaaa caacatttgg gccaccacca
      541 ggttagcagt gtgttcgttg acctcgtcgc ccaaaaagat gacgcggtca cgcagcaagc
      601 ggctgtagat gtcgtaagcc cgctcatagc ggccttcctg ctcaatgacg gttggaacta
      661 aaacttgtga tttcttatcc atagactacc taattgtatg gaactagcac tctcgtgtca
      721 agagtgctaa tttaacggtc tctttccttc tcgtcggagt cgcaaacctc ttgccgctga
      781 gactatactg ccagtcgcaa gcttgccact acttttcact gccctgggtt tccggcactc
      841 gttctcggct ttgatagact ctatgagagt agggcatccg atggcagctc ttacattcgt
      901 cggatcaaga ccaaaaacag gtagtgtgcc acaatccaaa gctaacccgc tagacccttc
      961 gttgaatagc tccaagtttt gagccacttg gccatagtca gcaatcactg gttttaatat
     1021 ctgcaaggtc ccgtattgtt ttttatacca gccaagctca ccccaatctt gacagcccga
     1081 acaaaggcac actgcttcaa aacttggctg aaacacattt ggggcgtatc tttctacaat
     1141 ctctcgctct gcccgagaga aatttcttcg atactgcctg cctccaaacg ggctacgtag
     1201 gtattttgcg cttatcggat ccctgatgtt cgtaaccccg tatttaccgg gattaaattt
     1261 cagatttgcg tactctatgc taagaacggg cgttgtaagc tcttcgtatt tatatttttc
     1321 ttccagccgg gcgttcccaa cgatagccgg atcgacacca aattgcagaa ggaagaaagc
     1381 ccgaccaagg acattaagaa catcttggat atactcactc tctcgctctt ctcggtgtga
     1441 ttccgtagct gaatatctgg cgtactcctg ctcggctaaa agcctttccg cctcctgctc
     1501 gcgagctatt tttaggaggg atcgttcctt atgcttatcg aacatagata tgataataaa
     1561 accacgattc ttactagctg gcaagtataa catgcgtaaa tggaatactt gacttgttag
     1621 gtattcattt gggtacgcgt tgcttggtag tgtaatcggt gagtttttcg agggtttttt
     1681 cggtcaggag gcggttagca atatcgttac ggttctccgg cttgtccagt tcggcgcgca
     1741 tctgggggtc ctggtactgg ccctttagca gctgcagtcg tatttccagc tcttccggag
     1801 tgactttaag gccctcttgc tcggcaattt cactcagcgc caggccggct ttgacgcgcc
     1861 cggcagccac cggcttgttg cgttcgcggt gctgttgctc gcttatcccc tcttccttga
     1921 ggtgttcttg ccaagtctgg ccgcggtagg tcaggttacg cttctcttcc tcttccatgg
     1981 ctagcacttg ttccgtgagc aggctctcag gaatgtctac ggtggtctta tctgcaatgg
     2041 tctgcaccaa ctggttttca taatcacggt cggcttggtt ctggcgttca atcttcagct
     2101 gctttttgat gtcggccttg agctcagcca gggttttaaa cggcccggcc ttggcggcca
     2161 gcgcgtcgtc cagttttggc tgggccaatt cggaaacttt tttagcatcc acctcaaaag
     2221 tgacatcttt gttctgcagc gctgccacgc cgtaattttt ggggaatttt acggtgaact
     2281 gtttggtctc accagctttc acgccaatta aattatcttc aaaaccggga atgaatgaat
     2341 tactgcccaa aatcaacggg tagtctttgc cgtcagcacc ggccaccggt tggccgtccg
     2401 aatctttacc ggaaaaatct atcagaatct cgtcgccgtt ttttgccggc cgctccacgg
     2461 gtttgcgctc cgccatacgt tcgcgcagcg atttcaatac ctcgttcaca tcttgagcgc
     2521 tgatttcaac cttaggctta gccagcttga tcttcttata atctggcagg attatcgggc
     2581 caatgacgtc cgtctcagcg ctgaattcca aatcagtaaa gggtacgaat tttttgacct
     2641 ctattttggg ctggccgacc ggccgcagtt tctggtggtc caccgcccgg cggtacagct
     2701 cgttaagggc gtggttgagg aactcgtcca acagcgcccg ctgatcaatg tgcttttcaa
     2761 ccacagacgc cggggcgtgg ccggcgcgga agcccggcac cttgacctgg ccagcaaaac
     2821 gccccagcac gtggcgctta atgggctcca gctcggcccc tgcagcgctg acactcaacg
     2881 acacgttggt tgagctgtta tctgtcctgg taatttgcat gagcccctat tatataattt
     2941 ttagcccaaa gtgataagtt aggcttataa ttcataacta agatatggca agaactcgca
     3001 accgaccccg ccaaagctcg cctcttaatc tgccgagcgc tttcgacctc tttaaaccca
     3061 gtaaagaaat cgtcctcaag aatatttgga tttttggtcc gctgtacgca
//
LOCUS       DASPTF010000061         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1827777, whole genome shotgun sequence.
ACCESSION   DASPTF010000061 DASPTF010000000
VERSION     DASPTF010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827777"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VEH48_01935"
     CDS             <1..302
                     /locus_tag="VEH48_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18162.1"
                     /translation="VPPSAVDNARSAVENAIAGGAYDPAFPTPVAALNAQPMGPELHQ
                     DNSNIPTPSLPLPTNGTAPQSADPAPPAPPAVPPPLPMNSAAVAALPPAAGQSLF"
     gene            332..1042
                     /gene="truB"
                     /locus_tag="VEH48_01940"
     CDS             332..1042
                     /gene="truB"
                     /locus_tag="VEH48_01940"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXY18163.1"
                     /translation="MDGFLLIDKPAGWTSHDVVAKVRGIINAGTGQKIKVGHTGTLDP
                     AATGLMVLVLGGYTKRASEFSKLDKTYEAELTLGATSSTGDSEGKITPTTGFNKSKYS
                     TRSRKILSYITEVVKSFEGTIWQKPPAFSAVKINGRRAYKLARAGKTVEIEPRQVKIY
                     EIRDVKYNYPKLSFTTSVSSGTYIRTLAADIGAKLGTGAYLSVLRRTSVGKFNIKKAL
                     ALDSLDLGKTRRGLLNHL"
     gene            1065..1331
                     /gene="rpsO"
                     /locus_tag="VEH48_01945"
     CDS             1065..1331
                     /gene="rpsO"
                     /locus_tag="VEH48_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXY18164.1"
                     /translation="MITGEKKAKITSDLASHKTDTGSSAVQVGIMTERIAELTAHMKT
                     HKHDFAARRSLLQLVGKRKRLLKYIAEQDGEAYLALIKKLGIRR"
     gene            1479..>2738
                     /locus_tag="VEH48_01950"
     CDS             1479..>2738
                     /locus_tag="VEH48_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18165.1"
                     /translation="MAETINPFGKKIIKVSTDFCGRELSLEVGRVGFRTSASVLARYG
                     DTVVLGTVMVSPEPVTGMDYFPLSIDYEEKFYAAGKISGSRFIKREGRPSDEAILIGR
                     LIDRPIRPLWPKGYRHEVQGVATVLSLDPDFRPDSIAMIAMSAAFMLTGVPFEGPIAG
                     VRVGLVDGKPKAFASVEQLEQGELDLVVAGTKDAIMMVEAGAREVSEDVVAEALEFAH
                     KAIQPAITLQEELIKKIGVTLQEYQLILPGEHVQKRVNEWVEGKMGKALRAPYPERND
                     LVSDLRHKLHEYLIEELGEDEFAASKADYDEAFDMAIHKDVRAGILDENIRPDGRKFT
                     EIRPLSSEVGFLPRAHGSSLFTRGVTQALNVVTLAPLSYAQLVDTMEREGERRYLHHY
                     NAPGYTVGEVRRLSGPGRREIGHGYLAE"
BASE COUNT          675 a          752 c          756 g          555 t
ORIGIN      
        1 tagtgccgcc ctcggctgtg gacaatgccc gcagcgccgt agaaaatgcc attgccggcg
       61 gagcttatga tccggctttc ccgactccgg tcgccgccct aaacgcccag ccgatgggcc
      121 cggaactgca ccaagacaac agcaacattc ctacgcccag cctgccgctg ccgaccaacg
      181 gcacggcgcc gcaatccgct gaccccgcac cgccggcccc gccggccgta ccgccgccgc
      241 tgcccatgaa ctcggcggcc gtggccgcac taccgccagc cgccggccaa agcctgtttt
      301 agcgcctgaa aacagtttat aatcgcgcct gatggatggt tttttattga ttgataagcc
      361 ggctggctgg acatcgcacg acgtggtggc taaagtccgc ggaattatta atgccggaac
      421 cggacagaaa ataaaagttg gccataccgg cacattggat ccggcggcca ccggcttaat
      481 ggttctggtt ttaggcggat acaccaagcg ggccagtgaa ttcagcaagc tggataaaac
      541 ctacgaagct gaactgacat taggcgcaac aagttcaacc ggagactccg aagggaagat
      601 cacgccgact acaggcttca ataaatctaa atattctacg cgatcgcgta aaatattaag
      661 ttatataacg gaggtggtaa agtcatttga gggaacaatt tggcaaaaac cgccagcttt
      721 tagcgccgtt aaaattaatg gtcggcgggc ctacaaattg gcgagggcgg gcaaaacagt
      781 tgaaatagag ccgcgccagg tgaaaatcta cgaaataaga gatgtgaaat acaattatcc
      841 caagctgagc tttaccacct ctgttagcag cggcacctat atccgcacgc tggccgccga
      901 catcggcgcc aagctcggca ccggcgccta cttatccgtc ctccgccgca cctccgttgg
      961 caaattcaat attaaaaaag ctctggctct ggactcatta gatttaggta aaacccgacg
     1021 ggggttgcta aatcacctct gatttgctat aattgcgcgt aagaatgata actggtgaga
     1081 agaaggccaa aatcacctct gatttagcct cgcataagac tgataccggc tcgtctgccg
     1141 tgcaggtcgg cattatgacc gaacgcattg ctgagctaac cgcgcatatg aagacgcaca
     1201 agcacgattt tgccgcccgc cgctcgctgt tacagctagt cggtaagcgc aaacggcttt
     1261 tgaagtacat tgccgaacaa gatggtgaag cctacttagc attgattaaa aagctcggga
     1321 tcagacgtta gatcttagac gccggacgct agaaataatg tccaaaatct agaatctaaa
     1381 ttctagtaac attaataagt gattgggtga tagagcacag gtttccgcta cgtgcggacg
     1441 cttggacctt gtcacccaag aaaggcaaat taacagatat ggcagagacc ataaatccgt
     1501 ttggtaagaa aataattaaa gtgagcaccg acttttgcgg ccgggagctc agcttggagg
     1561 ttggccgcgt cggttttcgg accagcgcca gcgtactggc gcgctacggc gacaccgtgg
     1621 tgctgggtac ggttatggtc agtccggagc cggtgaccgg catggattat ttcccgctaa
     1681 gtattgatta cgaagagaag ttttacgccg ccggcaaaat cagcggcagc cgcttcatta
     1741 agcgcgaagg tcggccgtcg gacgaggcca ttttaatcgg ccggctgatt gaccgcccaa
     1801 tccgcccgct gtggcctaag ggctaccggc acgaagtgca aggcgtggcc acggttttga
     1861 gcctggaccc ggacttccgg cccgattcta tcgccatgat agccatgagc gccgccttta
     1921 tgctgaccgg tgtgccgttt gaaggcccga tagccggcgt acgcgtcggc ctggtagacg
     1981 gcaagcccaa agctttcgcc agcgttgagc agttagaaca gggtgagctt gacctagtgg
     2041 tcgccggcac taaagacgcc atcatgatgg tggaagccgg cgcccgagaa gtcagcgaag
     2101 acgtggtggc cgaggctctg gaattcgccc ataaagctat ccagccggct ataactctgc
     2161 aggaagaact aatcaaaaaa atcggcgtaa cgcttcagga ataccagctc atcttgcccg
     2221 gcgagcatgt ccaaaaacgg gtgaacgagt gggtagaggg taaaatgggc aaggccttgc
     2281 gcgcgcctta ccctgagcgc aacgatttag tgtccgacct gcgccacaag ctgcacgaat
     2341 atcttattga ggagctgggc gaagatgagt tcgcggcatc caaggccgat tacgacgaag
     2401 cctttgacat ggccattcat aaagacgtgc gtgccgggat tttggacgaa aacatccgcc
     2461 ccgacggccg taagttcacc gaaatccgcc cgctttcaag cgaagtcggc tttttgccgc
     2521 gggctcacgg ttccagcctg tttacccgcg gcgtgaccca ggctttgaac gttgttaccc
     2581 tggcgcctct aagctatgcg cagctggtag atactatgga acgcgagggc gagcgccgct
     2641 acttgcacca ctataacgcg ccaggttaca ccgtcggcga agtccggcgc cttagcggac
     2701 ccggtcggcg cgaaatcggc cacggctatc tggctgag
//
LOCUS       DASPTF010000062        14814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1960518, whole genome shotgun sequence.
ACCESSION   DASPTF010000062 DASPTF010000000
VERSION     DASPTF010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 14814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14814)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14814
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960518"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /gene="ruvB"
                     /locus_tag="VEH48_01955"
     CDS             complement(<1..1010)
                     /gene="ruvB"
                     /locus_tag="VEH48_01955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HXY18166.1"
                     /translation="MAVDRIINTSTDEPEEERLEQTLRPRDFKNYIGQERLKKNLKLA
                     IDAAKKRGEPIDHILLYGPPGLGKTTLAGVIANEMGAQIRVTSGPAIERAGDLASLLT
                     NLQDGDILFIDEIHRLHRVVEEVLYAAMEDFKLDIMLGKGPSARSLRLDLPKFTIIGA
                     TTRTGALAAPLRDRFGMIHRLEFYTPEEISRIINRSAKILEVPIDKKSADLLSGRARL
                     TPRIANRLLKRVRDYADINGNGVIDENLATQALALLEIDDLGLDPADRHLLAVIMDHH
                     NGGPVGVETLAALTSEEKTTIEDFYEPYLMQIGLLERTPRGRKVTTRAWQHLNKKPAS
                     KN"
     gene            1058..1600
                     /locus_tag="VEH48_01960"
     CDS             1058..1600
                     /locus_tag="VEH48_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXY18167.1"
                     /translation="MEALDLVDESDQVIGRVPIDQAHKEGKLHREVHVWLYTPKAELI
                     WQCRGASKPMFASLLDATVGGHVTSGQTYEEAAIRELAEETGVIADIKQLRFIKTIRS
                     RIVDPRNGFINNVFLCVYALRYEGGLKDLKIEEGEISHFELWPIKKLFSLTEKEKQKF
                     IPALLSERNLEIYKAIQKLV"
     gene            complement(1592..2455)
                     /locus_tag="VEH48_01965"
     CDS             complement(1592..2455)
                     /locus_tag="VEH48_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18168.1"
                     /translation="MTIDLPDRKDREAILRVHFAEKPVGKDVDLDSLAAKTAGSSGAD
                     LANIANEAAILAARHDRKVIDNDDVVAAFEKVAIGPERKSKIMSDKEKELTAYHEAGH
                     AVVGHVLPDSDMVHKVTIIPRGQTGGVTWFIPPEDKSYHSIVEFKDVLARMLGGRIAE
                     ELIYGADRVTTGAGSDLQKAAELARDMVVNQGMGKKLRDQVFHTDEGMMLERLVHERT
                     YSDETAKIIDDEVESLITEAANRARIVLKANMAKLEELKKVLLEKETVEANEVIKILK
                     GTHLPKTALLN"
     gene            complement(2452..3462)
                     /locus_tag="VEH48_01970"
     CDS             complement(2452..3462)
                     /locus_tag="VEH48_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXY18169.1"
                     /translation="MDRKQFRLGNDGDNRRRNNFKNFGFIALLVLLGLIFFAAFNQHS
                     QLKSVPFSQVISEANSGKIQQITVKGDALEITPKGQSKATETSNKEDGSSIYQQGLQQ
                     GKVVLVNEPTSSSSSTWINLLLGTLLPVLLIGGLLIFMFRSAQGQGNQALSFGKSRAR
                     LYGNEKDRINFKDVAGNEEAKQDLQEVVEFLKYPKKFEGVGAKIPKGVLLVGPPGTGK
                     TMLARAVAGEANAPFFSISGSEFVEMFVGVGASRVRDLFNKAKKNAPCIIFIDEIDAV
                     GRRRGSGMGGGHDEREQTLNQILVEMDGFEQGQNVIVLAATNRSDVLDPALLRPAVLT
                     AG"
     gene            complement(3489..4370)
                     /gene="tilS"
                     /locus_tag="VEH48_01975"
     CDS             complement(3489..4370)
                     /gene="tilS"
                     /locus_tag="VEH48_01975"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXY18170.1"
                     /translation="MKILIPSGKYVVAVSGGVDSMVLLDLLAKKRDLELVVAHFNHGI
                     RADSDKDEELVRRTAKKLGIEFVAGHGRLGARASEEQAREARYSFLNAVKRQHRAKTM
                     ITAHHQDDLIETALINVLRGSGRLGLTSMLDNPAILRPLLNTPKKDILAYAAEHKVVW
                     REDSSNLDPKYLRNYLRLNILPRLTDSQRQELLKNIESVAKSQTELNALIATISHNVI
                     SHKIIDRGKYLSLPSPLDYELMAYWLRKNVGGDFNRKTVERLALGMKTAKPGSNLEAS
                     RGVYLVIGAKGAQFSNR"
     gene            complement(4372..4899)
                     /locus_tag="VEH48_01980"
     CDS             complement(4372..4899)
                     /locus_tag="VEH48_01980"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HXY18171.1"
                     /translation="MEQEVAKNSKPAMDYNPAEQPRTATDKAFRISLYLKGLDGLLEC
                     LGGVLLLLINPDQINHWAARLTQGELSEDPHDFIANHILKTAHHLTGASLVFGAVYLL
                     SHGVVKLVLVVEVLRNHLWAYLGLIGVTSLFVIYQLYRLLEEFSLGLTLLTVFDLAII
                     YLTQKEYRQRTAKAA"
     gene            4965..5041
                     /locus_tag="VEH48_01985"
     tRNA            4965..5041
                     /locus_tag="VEH48_01985"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4999..5001,aa:Met,seq:cat)
     gene            5589..5843
                     /locus_tag="VEH48_01990"
     CDS             5589..5843
                     /locus_tag="VEH48_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXY18172.1"
                     /translation="MYKLFVGGLPFATTDDELKELFAAHGSVASATVVRDRDSGRSKG
                     FGFVEFENDDEGKAAETALNGTEFGGRTISVAMARPKEER"
     gene            complement(5930..6670)
                     /locus_tag="VEH48_01995"
     CDS             complement(5930..6670)
                     /locus_tag="VEH48_01995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXY18173.1"
                     /translation="MAETVKPEEVASYTRMAGEIYDLIYSSKDYESQAAKVAELIKQR
                     CKSGGDRVLEAACGTGSYMKHLRNQFSIDGFDLSAEQVATAKKKLPENHIVQADMLNF
                     DMGQTYDAVLCLFSSIGYLKTKGNLDKAVANMARHLKPGGVVIIEPWLKPEDYIEGNI
                     SMESASSNGKLSVDRMGYSRREGNISVVDLHHMVGTERGVQHFLEVHELAMYTEDEFR
                     DAFTEAGLTFEVDPEGLIGRGLYIGNKP"
     gene            6866..7639
                     /locus_tag="VEH48_02000"
     CDS             6866..7639
                     /locus_tag="VEH48_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18174.1"
                     /translation="MLVIANTPTFYYSSPMPDLLREEYVEQYPLIGARNWRYKQEVSA
                     AVADHITDNVRRQRIAGHQIAQLLRNPPIGVSYAGTTRYCHPMVEKEMDWFEADIEET
                     MRRYEEAVSSRPERTAILVNGWAYRYEFERLGGSEPLWITQAMQLPAPETTKRNIGQP
                     SANLDVSVSASGMKNRSVGLFAEVSFGTENEYIDYSDVVILGKVDPDTGMAQTPYHTL
                     SDNIFDREHHYRGCRPVDGTLFPANRAANFILGQLEEIS"
     gene            complement(7653..7727)
                     /locus_tag="VEH48_02005"
     tRNA            complement(7653..7727)
                     /locus_tag="VEH48_02005"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7693..7695),aa:Met,seq:cat)
     gene            complement(7814..8278)
                     /locus_tag="VEH48_02010"
     CDS             complement(7814..8278)
                     /locus_tag="VEH48_02010"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXY18175.1"
                     /translation="MIDKSKNFWNSPRFRQLSDVRNIGLYIFALIVLAITWSGVKTVQ
                     NNYQLQKQIAELKQQNNVLYLQNGNIALQNQYYQTDQYLELAARQNFGLAAPGETVLL
                     VSRAVAMKYVDPSLAAKSNSINTNNLAGGPTGVAKNLEDWRDFLLGRKLFSD"
     gene            complement(8283..8358)
                     /locus_tag="VEH48_02015"
     tRNA            complement(8283..8358)
                     /locus_tag="VEH48_02015"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8323..8325),aa:Ile,seq:cat)
     gene            complement(8386..9333)
                     /locus_tag="VEH48_02020"
     CDS             complement(8386..9333)
                     /locus_tag="VEH48_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HXY18176.1"
                     /translation="MSILDKLPKPFLVLAPMDDVTDTVFRQIVADCAKPDLFFTEFVN
                     VDGLQSPGREKLLHKLAFSSKEKPIFAQLWGLKPENFKKTAAEVKAMDFDGVDLNMGC
                     PVKTVIKNGACAALINNRELTKAIIMATREGVGNDFPISVKTRVGFTTVDMGWIEFLL
                     GLKILDLLSIHGRTAKQLSKVPANWDLVGQARQMRDTLCPATLIVGNGDVMSRHQALE
                     LAKKYQLDGVMAGRGIFDDPFLFAQKSPWANFTKEQKIALFRKHVELFVKTYKKRERR
                     LETLRKFCKVYISGFDGAKDLREQIMEAASAEELLAVLS"
     gene            complement(9347..9823)
                     /locus_tag="VEH48_02025"
     CDS             complement(9347..9823)
                     /locus_tag="VEH48_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18177.1"
                     /translation="MSVEAPGQDQLEDPVEAAKRLIELTRSVGAETTESGEGESPFKI
                     HDRAQAQLDASKREFDTGWDHDDNSASGRNPTQTLDKLRKRGIYPTLTADEVQAAEEQ
                     KQAELRRAAAANKPPVKPKRPQKSARTHPYLSPGIRKLPGFDNEDDPSNPRSSRPF"
     gene            complement(9844..10416)
                     /gene="ruvA"
                     /locus_tag="VEH48_02030"
     CDS             complement(9844..10416)
                     /gene="ruvA"
                     /locus_tag="VEH48_02030"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HXY18178.1"
                     /translation="MIAVLDGLVTEKLGDVVILECAGVGYGLSVSFEDFGGLETGKPA
                     KLYIYEHIRDNAHDLFGFRKLETKILFEQLLSVNGVGPKMALAILSVANSQQVRKAIA
                     GGDTKFISQANGVGKRVAERVIVDLKDKVGLTADENATDFLTTAANPNDEALQGLVAL
                     GYSVQDAAEALKKVDTKLPPADRIKQALKT"
     gene            complement(10421..10897)
                     /gene="ruvC"
                     /locus_tag="VEH48_02035"
     CDS             complement(10421..10897)
                     /gene="ruvC"
                     /locus_tag="VEH48_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXY18179.1"
                     /translation="MRVIGIDPGTGILGFGVIETGKNGKVSLVDGGVIRTPIKEDDAV
                     RLQTIFDELTDIITQTKPTEMAVEKLFFAQNITTAMTVAQARGVVLLCGRQAGLKIAE
                     YTPLQIKQALTGYGRAEKKQMQEMVRVILGLREVPQPDDCADALAAAVCHSMNVRQ"
     gene            complement(10894..11718)
                     /locus_tag="VEH48_02040"
     CDS             complement(10894..11718)
                     /locus_tag="VEH48_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HXY18180.1"
                     /translation="MSELEEYLDFAKSLAEEAGKIMLKYFRAGLLETEIKDDLSPVTL
                     ADTEINSMVISRVKEKYPGHGVLGEEESFNLGNKQLWIVDPIDGTYNFASGLAGSVFS
                     LAFTEDGVVKVGVIYDPLLKALFWAAAGQGAYENNRKLDVSSGVPKGKLHISSWLAGD
                     INGTIFKDADMEGQSVVAYNRHGKIFVENWPVAHAIALVAAGRLDGSVTSCKNPWDLA
                     AGGLITQEAGAKVTDVYSNPISIWSKDIKGMVAAPPATHDILMEVISPVIESATPL"
     gene            complement(11711..12442)
                     /locus_tag="VEH48_02045"
     CDS             complement(11711..12442)
                     /locus_tag="VEH48_02045"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXY18181.1"
                     /translation="MSGHSKWATIKRQKGANDAKRGALFTKLGNQIAIAARSGTDPAT
                     NSALAMVVEKAKKASMPNANIERSIQRAADKSVAQLEEIMYEGYGPGGVAVLVECATD
                     NRNRTLPEVRSAFSKTGGNMAEPGSVAFQFSHKGVIRVKGTGEDTQLQAIEAGAEDVV
                     EEPETGETVIYTAQNDLAKVRDSLKGQGAEVVDAELTYVPNNIVEISDPETARKIINL
                     MNALDDLDDVTNTYTNFDIAEGVNV"
     gene            complement(12443..13804)
                     /locus_tag="VEH48_02050"
     CDS             complement(12443..13804)
                     /locus_tag="VEH48_02050"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HXY18182.1"
                     /translation="MLLIAFCLLLLRPKKITALLLVVFLGLSLGLWRGSAYMAHVRDL
                     RSLALQQVTIQATATSDSIYGTKSQIQFTANDAQLISLQGEPLQKGKSLVGSFKLSGF
                     GEHMVYRGDRVQMSGKLYPTRGSNQATIAYAQLTRVAAGRNWLNDIGRRFSAGMQNAL
                     PEPVASFAMGLLIGQRSTMPQDVITMLTMIGLVHIVAVSGYNVTILARAAARLKLPSK
                     YQQLVLSLTLIGGFVLLTGFSASIVRAALVSLLTLWAWFYGRRIKAVVLICFAAALTS
                     LLNPFYVWGDPSWYLSFLAFFGVLVIAPVITQRLFSKQPKLLTSVLIETLSAEVMTLP
                     LIMMIFGQLSLIALLANLLIVPLVPWAMLLAAVAGAAGMLAAPLAGWFALPARLLLTY
                     MLDVVHILANIPSEFLHLSISLPVMLSGYGLVLLVVLAAHRHIKLKNAIITDKKPILA
                     SEA"
     gene            14013..14088
                     /locus_tag="VEH48_02055"
     tRNA            14013..14088
                     /locus_tag="VEH48_02055"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14046..14048,aa:Lys,seq:ttt)
     gene            complement(14097..14579)
                     /locus_tag="VEH48_02060"
     CDS             complement(14097..14579)
                     /locus_tag="VEH48_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18183.1"
                     /translation="MADAILIALIAAPLVLSFLLRSNAALGFLVLCTGFTAATYASGS
                     TQHWLAHLASSGQILSTNSVNVIMIAVPLLITLLLTRKHRPRSKFYWEIIPALATGGL
                     LALAVEPFLNDWTNLNLSSLQLWKQLQNQEAVIIGVGALASLLMIWLSGGKLHTKKHK
                     "
     gene            complement(14603..>14814)
                     /locus_tag="VEH48_02065"
     CDS             complement(14603..>14814)
                     /locus_tag="VEH48_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HXY18184.1"
                     /translation="FWMKDMKFPIDMIWISSSHKAVVVEEDVKPSTYPDRFVNPVNHP
                     AQYVLELQAHASTRLGINPGTPIKF"
BASE COUNT         3403 a         3917 c         3724 g         3770 t
ORIGIN      
        1 tggttcttgc tggccggttt cttattaaga tgctgccaag ctctggtggt gactttacgg
       61 ccgcgcggcg tacgttccaa caggccgatc tgcatcagat aaggctcgta aaagtcctct
      121 atggtggttt tctcttcgga agtcagcgcc gccagggtct ccacgccgac cggcccgcca
      181 ttgtgatgat ccatgatcac tgccagcaga tgccggtcgg cgggatccag ccccagatcg
      241 tcaatttcca gcagcgccag cgcctgggta gccaggttct catcaatcac accattaccg
      301 ttgatgtcag cgtagtcgcg cacccgtttc agcaggcggt tggcgatacg cggtgtcaac
      361 cgggcccggc cagacaacag atcagccgat tttttatcaa tcggaacttc cagaatttta
      421 gccgaacgat tgataatccg gctgatctcc tcgggcgtat aaaactccaa acggtggatc
      481 atgccgaaac ggtcgcgcag cggcgccgct aaggctccgg tgcgggtggt ggcgccgata
      541 atcgtgaact tgggcaaatc cagccgcaaa ctgcgcgcgc tgggcccttt gcccagcata
      601 atgtccagct taaagtcttc catggcggcg tacagcacct cttcaactac ccggtgcagg
      661 cggtgaatct catcaatgaa taaaatgtcg ccgtcctgga ggttggtcag aagcgaggct
      721 aaatcgccgg cccgctcaat tgccggtccg gacgttacac ggatctgggc tcccatctcg
      781 ttggcaatca ccccggccaa ggtggtttta cccaaccctg gcgggccgta gagtagaatg
      841 tgatcaatcg gctcgccgcg ctttttagcg gcgtcaatcg ccagctttaa attcttcttc
      901 agccgttcct ggccgatata atttttaaaa tcccgcggcc gcagcgtctg ttccagccgt
      961 tcttcttccg gttcatcggt actggtatta attatcctat caaccgccat attaacagtt
     1021 taacactacg tttgaggcgt tttgttatga ttaagcaatg gaggctctgg atttagttga
     1081 tgagtccgac caagtaatag gacgggtgcc gatcgaccaa gcccacaaag aaggcaagct
     1141 tcatcgcgaa gtgcacgttt ggctctacac gccaaaagcc gagctaattt ggcaatgccg
     1201 cggcgcgtct aagccaatgt tcgccagttt actggacgct accgttgggg gacacgtcac
     1261 ttccggccag acctacgaag aagcggcaat cagagaattg gcggaagaaa caggcgttat
     1321 cgccgatatc aaacaacttc ggttcatcaa aacaatccgt tctagaatag tcgacccgag
     1381 aaacggcttt attaataacg tatttctttg cgtctatgcc cttcgctacg agggcggact
     1441 taaggacctt aagattgaag agggcgaaat aagccatttt gaactctggc caatcaagaa
     1501 gctctttagc ctcacagaaa aagagaagca aaaattcata ccggccctgt tgagcgagcg
     1561 gaatttagaa atttataaag cgattcaaaa gctagtttag cagcgcggtt ttgggcaggt
     1621 gtgtgccctt gaggatttta ataacttcgt tagcttcaac ggtttctttc tcaagtagga
     1681 cttttttaag ttcttccagc ttggccatat tggctttaag cacaatccgc gcccggttgg
     1741 cggcctcggt aatcaggctt tctacctcat cgtcaatgat cttagcggtt tcgtcagaat
     1801 aagtgcgttc gtgcaccagc cgctccagca tcatcccctc atcggtatga aaaacctggt
     1861 cgcgcagttt tttgcccatc ccctggttga ccaccatgtc gcgcgccagc tcggcggcct
     1921 tttgcaggtc actgccggcg ccggtagtta cgcggtcggc accgtaaatt aattcctcag
     1981 cgatccggcc gcccagcatt cgcgccagca cgtccttaaa ctcaactata ctgtgataac
     2041 tcttatcttc gggcggaatg aaccaggtta caccgccggt ctggcctcgg gggatgatgg
     2101 tgactttgtg caccatgtcg ctgtccggca ggacgtgccc gactacagcg tgaccggctt
     2161 cgtggtaagc tgtcagctct ttctctttat cgctcatgat cttgctcttg cgttccgggc
     2221 cgatggcgac tttctcaaaa gccgccacga cgtcatcgtt atcaataact ttacggtcgt
     2281 gccgggcggc taaaatggcc gcttcgttgg caatattagc taaatcggcg ccggatgagc
     2341 cggccgtttt ggccgccaaa ctatccaaat ccacatcttt accgaccggt ttttcggcaa
     2401 aatgcaccct taagatagcc tcgcggtcct tgcggtcggg caggtcaatg gtcacccggc
     2461 ggtcaaaacg gccgggcgca gcagtgccgg gtccagcacg tcactgcgat tggtagccgc
     2521 cagcacgatg acgttctgcc cctgttcaaa accatccatc tctaccagta tctgattaag
     2581 cgtctgctcg cgctcgtcgt ggccgccgcc catgccggaa ccacggcgcc ggccgacagc
     2641 gtcaatctca tctataaaaa tgatgcacgg ggcgtttttc ttggccttgt tgaacaggtc
     2701 acgcacccgg ctggcaccga cgcccacaaa catttctacg aactccgagc cgctgatact
     2761 gaaaaacggc gcgttggctt cgccggccac cgcccgtgcc agcatggtct taccggtacc
     2821 cggcggaccc accagcagaa cccctttggg aatcttagcg ccgacgcctt caaacttctt
     2881 cggatacttc aaaaactcca ctacttcttg caaatcctgt ttagcttcct cgttgccggc
     2941 cacatcctta aaattgatac ggtctttttc gttgccgtac aaccgggcgc ggctcttacc
     3001 gaaacttaac gcctggttgc cttggccttg ggcgctcctg aacataaaaa tcagcaaacc
     3061 gcctataagt aacaccggca gcagggtgcc cagcagcaaa tttatccagg tgctgctact
     3121 gctgcttgtc ggttcattga ccagtaccac tttgccttgc tgtagcccct gttgataaat
     3181 gcttgagccg tcttctttgt tggaggtttc tgtcgcttta ctctggcctt tgggggtgat
     3241 ttccagcgcg tcgcccttaa cagttatttg ctgaatcttg ccgctgttag cttccgatat
     3301 cacttgcgaa aatggcaccg acttgagctg gctgtgctgg ttgaatgccg caaaaaaaat
     3361 caggcccaat aatactaaca aggcgatgaa gccgaagttc ttaaagttat tccgccggcg
     3421 gttatcgccg tcgttaccca gtctaaactg tttcctatcc attctctgat attataacag
     3481 gctgtttgtc atctgttgct aaactgggcg ccttttgctc cgattactaa ataaacacct
     3541 ctggaagcct ccagattgct gccgggtttg gcggttttca tccccaaagc cagccgctcg
     3601 acggtcttgc ggttaaaatc accacctacg ttttttctca gccagtaagc cattagctcg
     3661 tagtccagcg gactgggaag tgaaagatat tttcctctat ctataatctt gtgactgatt
     3721 acgttatgcg atatcgtcgc aattaacgca ttcagttcag tttggctttt tgcgacactt
     3781 tctatatttt ttaacaactc ctgcctctgt gaatctgtca gccggggcag gatgttcaat
     3841 cgcagataat tccggaggta tttgggatca aggttgctgc tgtcttcgcg ccagacaacc
     3901 ttatgctcgg cggcgtaagc caggatgtct ttttttggcg tattcaacag cggtcgtaaa
     3961 atcgccgggt tgtccagcat tgatgtcaaa cccaagcggc cgctgccacg caagacatta
     4021 atcagcgccg tctcaatcaa gtcgtcctgg tggtgggccg taatcatggt tttggccctg
     4081 tgctggcgct tgacagcgtt caggaagctg tagcgggcct ctcgggcctg ttcctcgctg
     4141 gcccgagccc ccagccggcc atggccggcc acaaactcaa tcccgagctt ttttgccgtc
     4201 cgcctgacca actcctcatc tttgtccgaa tcagctctta tgccatggtt aaaatgggct
     4261 acaacgagtt ccagatcccg ctttttggcc agcaggtcaa gcagcaccat agagtcaacg
     4321 ccaccgctta cagctactac gtatttcccg gatgggatta agattttcat cctaagcggc
     4381 cttggctgtc cgctggcgat attctttctg cgtcaggtat atgattgcca aatcaaaaac
     4441 ggtcagtagt gtcagaccca gcgagaattc ctcaagcagc cggtaaagct ggtagatcac
     4501 gaataaagag gttacgccaa tcagccccag atatgcccac agatgattac gcagtacctc
     4561 aaccactaaa accagtttta cgacgccgtg cgacaacaga tataccgcgc caaagaccaa
     4621 agaggcacca gtcaggtgat gggcagtttt taagatatgg ttagcaataa aatcgtgcgg
     4681 atcttccgac agctcgccct gggtcagccg ggccgcccag tggttaatct ggtcggggtt
     4741 gattagcagc agcaagacgc cgcccaaaca ctccagcagt ccatccagcc ctttcaggta
     4801 caggctgata cggaaagctt tgtcggtcgc cgtccgcggc tgctccgccg gattataatc
     4861 catggcgggc ttactattct ttgccacctc ttgctccata tgtcgagtat agcaaacata
     4921 aaccccgcat ctcatctgtt ttatgctata atcacatctg ttaagccagg gtagctcaga
     4981 tggttagagc gcaggactca taagcctgag gtcgcgagtt cgatcctcgc ccctggtacc
     5041 agtgttttat gtataaaacg acctctcgag ctgtttttta tttttattaa aaacggccct
     5101 cgcatctgga tgcagtacaa ggaagcggcg aagcacggac tgagcagtat ttaaatactg
     5161 tgaaggagta ctggagccac tgacgccgta atgcgccaga aacgctggcg gtgcggccgg
     5221 ctgcgccggc tggcaggcta gcgtgctgcg taggccgttt attcttcgtc taaaacttcg
     5281 tctatggcta gtaactgcgg atcctcggtc ggcagcagca cgtacgctaa atcggcggac
     5341 atttttaccc cttaattttt tgttttttga tctgtcgcct atcatcatag cgtaacgcta
     5401 acgtttgtca acaacttttt atgcttgttt ttttggcgac cgcgtgttta actaccgctg
     5461 ttaggatctt atcggttctt ggtcaagaga gaacgagtga acggcccatc tgggaagtgt
     5521 ccaaagttcg accgtagcca gactagtaag gggtcgttcc tattcaatta agcacaagga
     5581 aattttagat gtacaaatta tttgtaggcg gtctgccgtt tgctaccacc gacgatgagc
     5641 tcaaagagct gttcgccgcc catggcagcg tggcttccgc cactgtagtc cgcgaccgcg
     5701 acagcggccg gtctaagggt tttggtttcg tcgagttcga aaatgacgac gaaggcaaag
     5761 ccgcggagac tgccttaaac ggcaccgagt tcggtggccg gaccatctca gttgcgatgg
     5821 cccgccccaa agaagagcgc taaggtttag tcgattcgaa acaaaacacc ctcatcgcga
     5881 gggtgttttt ggttatcgct tggtagagca ggtgcccacg caatggaggt tagggtttat
     5941 taccaatata gagtccccta ccgatgagtc cttctggatc aacctcgaag gttaaaccag
     6001 cttcggtgaa cgcatctcta aattcatcct ccgtatacat cgctaattcg tgaacttcaa
     6061 gaaaatgctg aacgccccgc tcggttccta ccatatgatg caagtcgacg accgatatat
     6121 tgccttctcg gcggctgtac cccatgcgat caaccgatag tttaccgttc gagctggcag
     6181 attccattga gatgttgcct tctatataat cttctggttt caaccatggt tcaataataa
     6241 ccaccccacc gggctttaag tgcctagcca tattagcaac ggctttatct agatttccct
     6301 tggtttttaa gtagccgatt gagctaaaaa gacacaagac ggcgtcatag gtttggccca
     6361 tatcaaagtt gagcatgtct gcttggacaa tatggttttc gggcagtttt ttcttcgctg
     6421 tagccacttg ctctgccgac aaatcaaaac cgtctataga gaactgatta cgcagatgct
     6481 tcatataaga tcctgtgccg caagctgctt ctaagactct gtcgccgcca gatttgcagc
     6541 gttgctttat aagttcagcc accttagcgg cctgactttc ataatcttta ctagaataaa
     6601 tcagatcata aatctcacca gccatccggg tgtagcttgc tacctcttct ggttttactg
     6661 tttccgccat tgttacataa taacaaaaag aggctcttca acctctttaa aatgcttatg
     6721 cttggtaggc gtgatagagt cgaattggaa ccaaatcaaa cgagaactga taactatgtg
     6781 gcatatagtt aaccagatta ggaaagaaag ccctgttgca gtctaagcag tagggtttgc
     6841 acttcataag ccataccaat acaaaatgct tgtgattgca aatacgccta ctttttacta
     6901 ttcttcgcct atgccagatt tattaagaga agaatatgtg gagcagtacc ctcttatagg
     6961 tgccagaaat tggcgatata agcaagaggt ttctgcggct gtagcagacc acattactga
     7021 taatgtgcgc cgtcagagaa tagctggtca tcaaattgcc cagctacttc gtaatcctcc
     7081 gataggagtt agttacgctg gtaccacaag gtattgccat ccgatggtag aaaaagaaat
     7141 ggactggttt gaggctgata tagaagagac gatgcgcaga tacgaagaag cagtttctag
     7201 cagacctgaa cgcactgcta ttctagtgaa tggctgggca taccgatacg agtttgagag
     7261 gctcggaggt agtgagcctc tttggattac gcaagcaatg cagttaccag caccagagac
     7321 taccaaacgc aatattggcc aaccatcggc aaacctagat gtatcagtat cggcatctgg
     7381 aatgaagaat cgtagcgttg gtttattcgc tgaggtatcg tttggcactg aaaacgaata
     7441 tattgattat tcggatgtag ttattctagg gaaggttgac cccgatactg gtatggcgca
     7501 aacaccttac cacacgctaa gtgataatat ttttgataga gaacatcatt atcgtggctg
     7561 tcgtccagtg gacggaacat tattcccagc aaatcgggca gccaacttta ttctaggtca
     7621 gctcgaagaa ataagttaaa agtgccagaa cttggtagcg ggggcaggaa ttgcacctgc
     7681 gacctcatgg ttatgagcca tgcgagctac tactgctcca ccccgcggcg agcgccgtca
     7741 ggcgcgagcc accccgagcg ctgtcgaggg acatcagacc aattacatat tctaatctgt
     7801 tataaggtgt ttgtcaatcg ctgaagagtt tgcggcccag cagaaaatcg cgccaatctt
     7861 ccaggttttt ggcgacgcct gtcggcccgc cagctaaatt gttagtgttg attgagttgg
     7921 attttgctgc cagggaaggg tccacgtact tcatcgccac ggctctagac accagcagaa
     7981 ccgtttcgcc gggcgcggcc aggccaaagt tctggcgggc cgccagctcc aaatactggt
     8041 ctgtttgata atactggttc tgcagagcga tattgccgtt ttgcagatac agcacgttat
     8101 tctgttgttt gagttcggca atctgttttt gcagctgata attgttctgc actgtcttaa
     8161 cgccgctcca ggttatggcc agcacaatca acgcaaagat atacagcccg atgttgcgca
     8221 catcgctcaa ctgcctgaat cgtgggctat tccaaaaatt tttagattta tcaatcatat
     8281 tctggtgggg gcggttggaa tcgaaccaac taccaagcgg ttatgagccg cctgctctaa
     8341 ccgatgagct acgccccctc cgctaccaag tataacagat gagaatcagc ttaggacggc
     8401 tagcagctct tccgctgatg cggcttccat aatttgttcg cgcaggtctt tggcgccgtc
     8461 aaagccgctg atgtaaactt tgcagaattt gcgcagagtt tccaggcgcc gctcgcgttt
     8521 tttataagtt ttgacaaata gctctacgtg tttgcgaaaa agcgcgattt tctgttcttt
     8581 ggtaaagttt gcccacgggc tcttttgggc aaacaaaaac gggtcgtcaa agatgccgcg
     8641 gccggccatc acaccgtcca gctgatactt tttagccagt tccagggctt ggtggcggct
     8701 cataacgtcg ccgttgccaa caattaacgt agcggggcag agggtgtcgc gcatttgccg
     8761 ggcctgaccg accaaatccc aattagccgg aactttggat aattgcttgg ctgtgcggcc
     8821 gtgaatactt aacaagtcaa gtatttttag ccccagcaga aattcaatcc agcccatgtc
     8881 aaccgttgta aatcctaccc gcgttttgac gctgataggg aagtcattgc ctaccccttc
     8941 cctggtggcc ataataatcg ctttggtcag ttccctgttg tttatcaaag ccgcgcaggc
     9001 gccgtttttg atgacagttt tgactgggca gcccatgttc aaatctaccc catcgaagtc
     9061 catcgcctta acttcggcgg cggttttctt aaaattttcg ggcttcaggc cccacaactg
     9121 ggcaaaaatc ggcttttctt tggagctgaa ggccagtttg tgcagcagct tttccctgcc
     9181 cgggctctgc aagccatcta cgttcacgaa ctcggtaaaa aacagatcgg gcttagcgca
     9241 atcggcgaca atctggcgga aaaccgtgtc cgtgacatcg tccatcggcg ccaaaaccaa
     9301 aaacggcttt ggcaacttat ccaagatgct cattcgttat atcttactaa aatggacgag
     9361 aactccgagg gttactgggg tcgtcctcgt tatcaaaacc tggtaattta cggattcccg
     9421 gactcaggta tggatgtgtc cgggctgatt tttggggacg cttgggcttg actggaggct
     9481 tgtttgccgc tgccgcccgg cgtaattcag cttgtttttg ttcttctgcc gcctggactt
     9541 cgtcagccgt gagtgtcgga tatatacccc gctttctcag cttgtctagg gtttgcgtgg
     9601 gatttctgcc gctggcagaa ttgtcatcgt gatcccaccc ggtatcaaac tccctcttgg
     9661 atgcgtctag ctgtgcttgc gctctatcat ggatcttgaa ggggctttct ccttcgccgg
     9721 attctgtcgt ttctgcgcca acgcttctag ttagttcgat aagtctttta gccgcttcaa
     9781 ctggatcttc aagttgatct tgtccgggtg cttctactga catacttata ctttagcaaa
     9841 tgtttatgtc ttgagggctt gtttaatacg gtcggcgggc ggcagtttgg tgtcaacctt
     9901 tttgagggct tcggcggcgt cttgcacgga ataaccgagg gcgactaacc cttgcagggc
     9961 ttcgtcgttg gggttggcgg ccgtggtcag gaagtcggtg gcgttttcgt cggccgtcag
    10021 gccgacttta tctttcaaat ccacgatcac gcgctcagct acacgcttcc ctactccgtt
    10081 ggcttgtgag atgaatttgg tatcgccgcc ggcaatggct ttgcggactt gctgtgaatt
    10141 ggccacgctt aggatcgcca gcgccatttt tgggccgacg ccattgacgc tcaaaagctg
    10201 ttcaaacaga attttggttt ccagtttgcg gaagccgaag agatcgtgag cgttgtcgcg
    10261 gatatgttcg tagatgtaaa gtttggccgg cttgcctgtt tccaggccgc caaaatcctc
    10321 aaaactgaca cttagaccgt agcccacgcc ggcgcactca agaataacca cgtcgcccag
    10381 tttttcggtt actagcccgt ctaaaaccgc tatcatggcc ttattgtctc acattcatgc
    10441 tgtggcaaac cgcggcggcc aaggcgtcgg cgcagtcgtc gggctgcggc acttctctga
    10501 gccccagaat tacccggacc atctcctgca tctgtttttt ttctgcccgg ccgtaaccgg
    10561 tcagcgcctg ctttatttgc agcggcgtat attcggcgat tttgaggccg gcttggcggc
    10621 cgcacagcag taccactccc ctggcctgtg ccaccgtcat agcggtagtg atattttggg
    10681 cgaaaaacag tttttccacg gccatttcgg tcggcttggt ttgggtaatt atatctgtta
    10741 gctcatcaaa gatggtttgc agacgcacag catcatcttc tttgattggc gtgcgaatga
    10801 cgccaccatc aaccaaactg accttgccgt ttttgccggt ctctataacc ccaaacccca
    10861 aaatccccgt ccccggatca ataccgatga ctctcacaac ggtgtagcgc tttctatcac
    10921 tgggctaata acttccatga gtatatcgtg ggtggccgga ggcgcagcta ccataccttt
    10981 aatatccttg ctccagatgg atattggatt gctgtagaca tcagtgactt tcgcgccggc
    11041 ctcctgagta ataagaccac cggcagccaa gtcccaggga tttttacagc tggtaacact
    11101 accgtctaag cggccagcag cgaccagcgc gatggcatgg gcaaccggcc aattttcgac
    11161 aaaaatcttt ccatgtctgt tgtaggcgac tacggattgg ccctccatat cagcatcttt
    11221 gaagatagtg ccgtttatat cgccggccaa ccaagatgat atgtgcagct tgccttttgg
    11281 cacgccgctt gagacatcca gtttacgatt attctcataa gccccttgcc ccgcggcggc
    11341 ccagaataag gccttgagca ggggatcgta aatgactcca accttgacca ccccgtcttc
    11401 tgtgaaggcc aagctaaaga ccgatcctgc caatccgctg gcaaaattat aagtaccgtc
    11461 tatagggtcg actatccaaa gttgcttatt gccgaggttg aaactttctt cttcgcccaa
    11521 aacaccatgg ccgggatatt tttccttaac cctggatata accatcgaat tgatctcggt
    11581 atccgcgagc gttactggcg ataaatcgtc tttaatctcc gtttcgagta agcctgcgcg
    11641 gaagtatttg agcattattt taccggcctc ttcggctagc gacttggcaa aatccaaata
    11701 ttcttccaat tcagacattt acgccttctg ctatatcgaa gttggtgtag gtgttggtca
    11761 cgtcgtccaa atcgtctaac gcgttcataa ggttgatgat tttgcgggcg gtctcggggt
    11821 cgctgatttc tacgatgtta ttcgggacat atgtcagttc ggcatccacc acttccgcgc
    11881 cctgcccttt tagcgagtcc cggactttgg ccaaatcgtt ttgagcggta taaattacgg
    11941 tttcgccggt ctccggctct tctacaacat cctcggcgcc ggcttcgatg gcttgcagct
    12001 gtgtgtcttc accagttccc ttcactctta tcacgccctt gtggctgaac tggaaggcca
    12061 cgctgcctgg ctcggccatg ttgccgccgg ttttgctgaa ggccgagcgg acttccggta
    12121 atgtgcggtt gcggttatcg gtggcgcatt ctaccagcac agccacgcca cctggaccgt
    12181 agccttcgta cataatttcc tctagttgcg ctacggactt gtcggctgcg cgctggatgg
    12241 agcgctcaat gttggcattg ggcatgctgg ctttcttggc cttttcgacg accatagcca
    12301 gcgccgaatt ggtagccggg tcggtaccgc tgcgcgcggc aatggcaatc tgattaccca
    12361 acttggtgaa caacgcgccg cgcttggcat cgttggcgcc tttttgccgc ttgattgtcg
    12421 cccacttaga gtgaccagac atctatgcct cgcttgctaa aattggtttc ttatctgtta
    12481 ttatagcatt tttgagcttg atgtgccggt gggcggcaag gacgactaaa agcaccaagc
    12541 cgtagccgct taacatcacc ggcaggctga tggacagatg caggaactcc gatgggatat
    12601 tcgccagaat atggacgaca tccagcatat aggtcagcag caggcgggcc ggcagggcga
    12661 accagccggc cagcggtgcg gccagcatgc cggccgcacc ggcgaccgcc gccagcagca
    12721 tcgcccaagg cacaagcggc acaatcaata aattggccag caaggctatt agtgacagct
    12781 ggccgaaaat catcataatc agcggcaggg tcatcacttc ggccgagagc gtttcaatca
    12841 aaactgaagt taacagttta ggctgcttgc taaacagacg ctgggttatc accggcgcaa
    12901 tcaccaatac gccgaagaag gccaggaacg acagatacca gcttgggtcg ccccaaacgt
    12961 aaaacgggtt cagcagagag gttagcgcgg cagcgaaaca gattagaacc accgctttga
    13021 ttcgccggcc gtagaaccag gcccacaagg tgagcaaaga caccagagcc gcccggacga
    13081 ttgaggcact aaaaccagta agcagaacaa agccgccaat caaagtcagc gacagaacca
    13141 gttgctggta cttgctgggc agcttgagcc tggctgcggc ccgcgccaga atcgtcacgt
    13201 tgtagcctga aaccgccacg atatgcacta gaccaatcat ggtcaacata gtgattacgt
    13261 cttgcggcat ggtggagcgc tggccgataa gcaagcccat ggcaaaagat gctacgggtt
    13321 caggcaaggc attctgcatg ccggcagaaa aacggcggcc gatatcgttc agccagttac
    13381 ggccggcggc cacccgtgtc agctgggcgt aagcgatggt ggcttggttg gagccgcgcg
    13441 taggataaag tttgccgctc atctgcactc tgtcgcctct gtaaaccatg tgctcgccaa
    13501 agccgctgag tttgaatgac cccaccaatg acttgccttt ctgcaagggt tccccttgta
    13561 gggagatgag ctgggcgtca ttagctgtga actgtatctg cgattttgtg ccgtaaatgg
    13621 aatcgctggt ggccgtagcc tggatagtaa cttgttgcag ggccagactc cgcaaatccc
    13681 gcacgtgcgc catgtaagcg ctgccgcgcc acagccccaa gctgagacct aaaaaaacta
    13741 ccagcagtag agcggttatt tttttgggtc tcagcagcaa taagcaaaaa gcaataagca
    13801 acatgggcca agtcgagagg tcaaggctaa ctttacgcga caagcctaga ccggccaaaa
    13861 ctgccaaaca gccagctgta ataagagttg tgcgccgaaa cctccgcatg atccctaatc
    13921 atcccagaaa aaacccgata aattaattgc ttacggttga tttttgccgg aaaacaacag
    13981 ataatataag gtgttaattc gcctgtcaat acgggcccat agctcagttg gtagagcaga
    14041 ggccttttaa gcctcgtgtc gcgggttcga gacccgctgg gccctccagg ctttttttat
    14101 ttgtgttttt tggtatggag tttgccgcca gacagccaga tcatcagcaa actggccagc
    14161 gcgccgacgc cgataataac ggcttcttga ttctgcaatt gcttccatag ctgcagggag
    14221 ctgagattca agttggtcca atcgttcaga aacggctcca cggccagggc caaaagcccg
    14281 ccggtggcca gagctgggat aatttcccag taaaattttg agcgcggacg gtgttttctg
    14341 gtcaaaagta atgttataag cagcggcacg gctatcataa tcacgttgac cgagttggtg
    14401 cttaggattt gccccgagga cgccaagtgg gccagccagt gctgggtact gccgctggca
    14461 taagtggctg ccgtgaagcc agtgcaaagc accaaaaacc ccaacgcggc gttggagcgt
    14521 aggagaaagc tcaagaccaa aggcgcggca atcagcgcga taagaatcgc atcggccata
    14581 aggccatttt agcactaacg gtttagaact ttatgggtgt tcctgggtta atacccagcc
    14641 ttgtggaggc gtgggcttgg agctccagaa catactgggc cggatggtta acggggttga
    14701 cgaacctatc ggggtaagtt gaaggcttaa catcttcttc tacgactact gctttatgag
    14761 aggaggaaat ccaaatcatg tctataggga acttcatgtc tttcatccaa aact
//
LOCUS       DASPTF010000063         4186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1960682, whole genome shotgun sequence.
ACCESSION   DASPTF010000063 DASPTF010000000
VERSION     DASPTF010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960682"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VEH48_02070"
     CDS             <1..190
                     /locus_tag="VEH48_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18185.1"
                     /translation="ILGDSTGDNQKTVQNLSDYSAAGWYTNNYLLLSKNGSELYISDL
                     KGDTPIKITDYQPGTFAL"
     gene            complement(213..851)
                     /locus_tag="VEH48_02075"
     CDS             complement(213..851)
                     /locus_tag="VEH48_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HXY18186.1"
                     /translation="MDKKLRAAEKHLASRDKKLAPVIKAYNPCRIVPHSDHYGELVSS
                     IVGQQLSTRAGATIWRRVLDLFGGKMPTPRQLIEVEADKVRACGVSYPKIGYMKDLAR
                     HVLDGRLDLPHISTMPNDQLIEQLTAVKGIGEWSAHMFMIFGLGRLDVLPVGDLGVRK
                     AIMNLYGLKRLPEPAAIITLSNKNGWHPYESVAAWYLWQSLDNSPQKLTRHS"
     gene            912..1310
                     /locus_tag="VEH48_02080"
     CDS             912..1310
                     /locus_tag="VEH48_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18187.1"
                     /translation="MQPKKSPYIRDYKASEPQPAALPPDEQVKTEVVSNIPVQQQSAS
                     ANPISTVPVTPQNNDKDLDKILTDVNREVGKQWAEQDKKRLPLPVPQKKVDKKPAGGS
                     RSHILPIAIAIIIAIALAAAAVSAFKSGGA"
     gene            1314..1946
                     /locus_tag="VEH48_02085"
     CDS             1314..1946
                     /locus_tag="VEH48_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXY18188.1"
                     /translation="MKITKFVHSCLLVETPNQTVLIDPGNYSYESRLLRLAAMPNLDY
                     IVITHEHPDHFHPPFLQEICAKFPYATIITNEDLAGKLAAEKLRNPVSTGSQEGIEVF
                     PAAHEPLPLDLPSPAHIGVHIENFLTCPGDALDIKSRDVLALPLTAPWGSVKMALDSA
                     VKVKPKKVIPVHDWHWHKAAREAMYAMSKELLAPHGIEFIELENAVPVEL"
     gene            complement(1953..3266)
                     /locus_tag="VEH48_02090"
     CDS             complement(1953..3266)
                     /locus_tag="VEH48_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HXY18189.1"
                     /translation="MHVYFSGIGGSGIAPLALIAKQAGYQVSGSDGRPSQYLEYLKKH
                     GIADTHMGVDRDFIAAVHAKQPIDWFVYGSAQPMDFPDHPEFAFCQENHIKMSKRDEF
                     LSRIITDKKLKLIAVAGTHGKTTATAMAVWLFKQLGIPVSYSVGGKINFGETGQFDPA
                     SKYFVYEADEYDRNFLSFYPEIALIAGIDWDHPDIYPSREEYYAAFRQFLSQSRQNFI
                     WRGDAKKLAVPASDSVTLIDEQDPQIDKQVVLAGHVNRLDAWLVTTGLVKPLDKPAPE
                     LVAVLNKFPGLARRFEQLAPNLYTDYAHTPAKIRGALQMAREILTGGTLAGKVVVVYE
                     GLHNTRQHFIKDQLADLFDGVKQLYIVPSYLAREDPKLELLTPEKILDLLSNSTKGHA
                     AAAQLNDDLASKIRQHTADGDLVLCFSAGSGDSLDEWLRKTFAPR"
     gene            3351..3941
                     /locus_tag="VEH48_02095"
     CDS             3351..3941
                     /locus_tag="VEH48_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18190.1"
                     /translation="MRSVKKYLRKAWRAPLWAFAAAFLISSSVSVLALRHNNQTMIKL
                     RNALYAADKNNGDVNAALNNLRNYVYAHMNTNLSSGGNTIKPPIQLKYTYQRLVQAQQ
                     QQLDSQNSQLYTQAEDYCQAQDSTDYYGYYRVPCVENYLTNHGLRTADTSVPAGLYEF
                     DFLSPAWSPDLAGWSLAVSSLMLLLLIVKLMTVRFK"
     gene            complement(3989..>4186)
                     /locus_tag="VEH48_02100"
     CDS             complement(3989..>4186)
                     /locus_tag="VEH48_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18191.1"
                     /translation="RSQVAFWENLQDSGKVVQAIQQYIKQNPNGPNCSQSSSSSSTAP
                     SSTTPQSSGSTGTSSNTTSGQ"
BASE COUNT          986 a         1148 c         1115 g          937 t
ORIGIN      
        1 gatactgggc gacagcaccg gcgacaacca aaaaaccgtc caaaacctca gcgattacag
       61 cgccgccggc tggtacacca ataactatct gctgctgagc aaaaacggca gcgagctcta
      121 tatcagcgac ttaaaaggtg atacgcccat taaaattacc gactatcagc ccggcacctt
      181 cgctctatag aaatacttga ctagtcaagt atttagctgt ggcgcgtcag cttttgcggg
      241 gaattgtcca ggctttgcca gaggtaccag gcggcaaccg attcgtacgg gtgccagccg
      301 tttttgttag acaatgtgat gattgccgcc ggttccggca gccgcttaag cccgtacaag
      361 ttcatgatgg cttttctgac gcccagatcg ccgactggca aaacatccag ccggcccagg
      421 ccaaaaatca tgaacatatg ggcgctccat tcaccgatac ctttgacggc cgtcagctgc
      481 tcaatcagct ggtcgttagg catggtagag atgtgcggca gatccagccg gccatccagc
      541 acgtgccggg ccaggtcttt catgtagccg attttggggt agctgacgcc gcaggcccgc
      601 actttgtcag cctccacctc tataagctgg cgcggcgtgg gcatcttgcc gccgaataaa
      661 tccaaaaccc gccgccagat agtggcgccg gccctggtgc tgagctgctg gccgacgatg
      721 ctgctgacca gctcgccgta gtggtcactg tgcggcacta tccggcaggg gttgtaagct
      781 tttatgaccg gcgccagttt tttgtcgcgg ctagccaaat gcttttccgc cgctcgcaac
      841 tttttatcca tgttgaatat taaagcagac tgcggtctga tacatcaggc ttatgctaaa
      901 atacctctgt aatgcagcct aaaaaaagcc cttacatccg cgattataag gcgtcggaac
      961 cccagccagc cgcactgccg cccgacgagc aggtaaaaac cgaggtagtc agcaatattc
     1021 cggtacagca acagtcggca agcgccaatc ccataagcac tgttccagtt actccccaga
     1081 acaatgacaa agatctggac aaaatcctca ccgatgtcaa ccgcgaggtc ggcaagcaat
     1141 gggccgagca ggacaagaag agactgccgc tgccggtgcc gcaaaaaaaa gttgataaaa
     1201 aacccgcggg cggcagcaga agccatattt tacctatcgc tatcgccata atcatagcca
     1261 tcgccttggc cgccgccgcg gtctcggcct ttaagtccgg tggagcttaa ggcgtgaaaa
     1321 tcactaagtt tgtccactcc tgcctactgg tggagacacc caaccaaact gttctgattg
     1381 atccgggcaa ctattcttac gaaagccgcc tgctgcggct ggcagcaatg ccaaacctgg
     1441 actatatcgt catcacccat gagcatcccg accacttcca cccgccattt ttgcaggaga
     1501 tctgcgccaa attcccgtac gccaccatca tcactaatga agacttggcc gggaaactgg
     1561 ccgccgaaaa gctgcgcaac cctgtcagca ccggcagcca ggaggggatt gaagttttcc
     1621 cagcggccca cgaaccgctg ccattagatt tgcccagtcc ggcgcatatc ggcgtgcata
     1681 tagaaaactt tttgacctgc ccgggcgacg ctttggatat aaaaagtcgt gatgtcttag
     1741 ccttgccgtt aacggcaccg tggggcagcg tgaaaatggc gctggactca gctgtaaaag
     1801 ttaaacccaa aaaggtcatt cccgttcacg actggcattg gcacaaagcg gcgcgtgaag
     1861 ccatgtacgc catgagcaaa gaattgctgg cgccgcacgg cattgaattt atagaactgg
     1921 aaaacgccgt cccggtcgaa ctttaaacta ggctagcgcg gtgcgaacgt cttgcggagc
     1981 cattcgtcca gggaatcgcc gctgccggcg ctgaaacata gaaccaggtc gccgtcggca
     2041 gtgtgctggc ggattttact ggccagatca tcgttgagct gcgctgccgc ggcgtggcct
     2101 tttgtcgaat tacttaacaa gtcaagtatt ttctcagggg tcagaagttc taatttgggg
     2161 tcttctcggg ccaggtagct tggcacgatg taaagctgct taacgccgtc aaacaaatcc
     2221 gccagctggt cttttatgaa gtgctggcgg gtattgtgca gaccctcata aaccacaacg
     2281 actttccctg caagggttcc ccctgtaagg atttcgcggg ccatctgcag ggccccgcgg
     2341 attttggccg gcgtgtgcgc gtaatcggtg tataagtttg gcgccagttg ttcaaaccgc
     2401 cgcgccaggc cggggaactt attgagcacc gccaccagtt ccggcgccgg cttgtccagc
     2461 ggcttaacta aaccggtggt gaccaaccag gcgtccagcc ggttgacgtg tccggccaga
     2521 acgacttgtt tgtctatttg cgggtcttgc tcgtcaatca gcgtcactga gtcggaagcc
     2581 ggaaccgcca gttttttggc gtcgccgcgc cagatgaaat tctgccggct ttggctcaag
     2641 aactgcctga aagcggcgta atattcctcg cgcgacggat aaatatccgg gtggtcccaa
     2701 tctatgccgg caatcagcgc gatttccgga taaaaactca aaaaattgcg gtcgtactca
     2761 tcggcttcgt aaacgaagta tttgctggca gggtcaaact ggccggtttc gccaaaattg
     2821 atcttaccgc cgacggaata gctgacgggg atacctaatt gtttgaacag ccacaccgcc
     2881 atggccgtag cggtggtttt gccgtgcgtg ccggcgacgg ctatcagctt gagctttttg
     2941 tcggtgataa tccggctcaa aaactcgtcg cgcttggaca tcttgatgtg gttctcttgg
     3001 caaaaggcga attccgggtg gtcgggaaaa tccatcggct gggcagagcc atagacaaac
     3061 caatcaatcg gctgtttggc gtgtacggcg gcgatgaaat ctcggtcaac acccatgtgc
     3121 gtatcggcga taccgtgctt tttcaagtat tcaaggtatt ggctgggccg gccgtcagaa
     3181 ccgctgactt ggtagccggc ctgcttggct attaacgcca gcggtgcaat acctgaaccg
     3241 ccgatgccgg aaaaatacac atgcatatta atagggtata gggtttaggg ggtagggtct
     3301 agggtaatca attttgacga tcagggctgc gcatgacaac ataagaggta atgcgcagcg
     3361 ttaaaaaata tctgagaaag gcctggcggg caccgctgtg ggctttcgcg gcggcattct
     3421 taatcagttc ttcggtatct gtactagcat tgcggcacaa caaccagacg atgatcaaac
     3481 tacgcaacgc cctttatgcc gctgataaaa ataacggtga tgtcaacgct gcgctaaaca
     3541 atctgcgcaa ttatgtttac gcccacatga acaccaacct atcaagcggc ggcaacacca
     3601 tcaaaccacc tatccagctg aaatacacct accagcggct ggtccaggcc caacagcagc
     3661 agctggacag ccagaactct cagctctaca cccaggccga agattactgt caggcccagg
     3721 actccaccga ttattacggt tattaccgtg tgccgtgcgt ggaaaattat ctgacgaatc
     3781 atggcctgag gacggccgac accagtgtgc cggctggact ttacgaattt gattttctaa
     3841 gccccgcctg gtcgccggac ctggccggct ggagcctagc ggtaagctcg ctgatgctat
     3901 tgttactgat cgtcaagctt atgactgtcc ggttcaaata acaataaaac ccctcgggtg
     3961 aggggtttta tccggactgt ttgtttgact actgaccgct ggtagtgttg gaactggtac
     4021 cggtggagcc gctactctga ggtgtagtag aagaaggcgc agtgctggag ctactgctgg
     4081 actggctgca gttaggtcca ttcgggttct gcttgatgta ttgctgaata gcctgtacaa
     4141 ccttgccgct gtcttgcagg ttctcccaga aagccacttg cgaacg
//
LOCUS       DASPTF010000064         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_2108301, whole genome shotgun sequence.
ACCESSION   DASPTF010000064 DASPTF010000000
VERSION     DASPTF010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108301"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VEH48_02105"
     CDS             <1..744
                     /locus_tag="VEH48_02105"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18192.1"
                     /translation="KAGFDEFALFELGKGHNKIHPTAGREKVPAEFQMLSLVFASRRK
                     SAKANGAPFYQARAILDYLARELGITLEYRPIVTEEPYPVVKPFDHTRSAQIWEAKTN
                     TPLGMVGEYKQSVIGNLKLPQYSAGFEIGIDQLLQVAPPHDAYRPLNRYPEVGQDFCL
                     RAPADLDYAELTELIVKSLTELTKPHGYEFWWRPLDIFQKAGDKTRKQITWRIILFHP
                     ERTLTTQETNKLLDELAARAKKELKAERI"
     gene            745..1359
                     /locus_tag="VEH48_02110"
     CDS             745..1359
                     /locus_tag="VEH48_02110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXY18193.1"
                     /translation="MQKPEVSGEKVKSGLRRAMWLFMAMLFLVTGLGVGVYAFWEGTH
                     QNNTSSTTTPNTQSQVPACTNDPTLQIAGSSASGGKLAGTKLVNFTPIAHIASLSCID
                     AKIGTGTATVQSTSTITANYTGAVAATGKIFQSSLDNNGQPFSTQLSGVIPGWQEGLL
                     GMRAGGERRLLVPAALAYGANPPAGSGIPANADLVFDITVLAVQ"
     gene            1480..1728
                     /locus_tag="VEH48_02115"
     CDS             1480..1728
                     /locus_tag="VEH48_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HXY18194.1"
                     /translation="MTKNITIFTTNTCSYCGMVKRYLDMKGMSYDVVNLDEHPERQAE
                     VYDMSGALTVPITVVTKHDDSKEVVVGYNLSRLAPAVA"
     gene            1738..>2214
                     /locus_tag="VEH48_02120"
     CDS             1738..>2214
                     /locus_tag="VEH48_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXY18195.1"
                     /translation="MHDLVIVGAGPAALSAALYTSREDIDTLLFEKGAIGGLAAVTDW
                     IDNYPGFAKGIAGLELSDAMRAQAERFGAKIELGEVSKITKDGDLIKLDTTSGEIKAK
                     AVLIATGSDYRKIGVPGEQEYYARGVHYCATCDGAFYRDKRLVVVGGGNSAVQESMF"
BASE COUNT          568 a          617 c          585 g          444 t
ORIGIN      
        1 aaagccggct ttgacgagtt cgctcttttt gaactcggca aaggccacaa taaaattcat
       61 ccgaccgccg ggcgcgagaa agtcccggcg gaattccaaa tgctctcgct agtatttgca
      121 tccagacgaa aatcagccaa agccaacggc gcgcctttct atcaagcccg cgccatactg
      181 gactatctgg ccagggaact gggcatcact ttagaatacc gtccgatcgt gaccgaagag
      241 ccttacccgg tggtcaaacc atttgaccat acgcgctcgg cccagatctg ggaagctaag
      301 actaatacgc cgctgggcat ggtcggcgag tacaaacagt cggttatcgg caatctcaaa
      361 ctgccccaat actcagccgg ctttgaaatc ggcatagacc agcttctgca agtggccccg
      421 ccgcacgatg cctaccggcc gctcaaccgc tacccggaag tggggcagga tttctgcttg
      481 cgggccccag cagaccttga ctacgccgag ctgaccgaat taatcgtcaa aagcctaaca
      541 gagcttacta aaccgcacgg ttatgaattc tggtggcggc cactggatat cttccaaaaa
      601 gccggcgaca aaacgcgcaa gcagatcact tggcgtataa ttctattcca tccggagcgt
      661 acgctgacca cgcaggaaac caataaactg ctggacgagc tggcggcccg ggccaaaaaa
      721 gaactgaaag cggagaggat atagatgcaa aaaccagaag tcagcggcga aaaagttaaa
      781 agcggcttac gccgggcgat gtggctgttc atggccatgt tgtttctggt caccggcctg
      841 ggcgtgggag tctatgcctt ttgggaggga actcatcaga ataatacctc ttcaaccacc
      901 actcccaata cgcaatcgca ggtgccggct tgtacgaatg acccaactct gcaaattgcc
      961 ggctcttctg ccagcggcgg taagctggcc gggacaaaac ttgttaattt tacgcctatc
     1021 gcgcatatag cttcacttag ctgtattgac gctaagatcg gaactggtac cgcaacggtg
     1081 cagtccacaa gcacaattac agctaactac accggcgcgg tggcggctac cggtaagatt
     1141 ttccaaagct cgcttgataa caatggacaa ccgttcagca cccagcttag cggcgtcatt
     1201 cctggctggc aagaaggtct tctgggtatg cgtgccggcg gcgaacggcg gttgttagta
     1261 cccgcggcgc tggcttacgg cgccaacccc ccggccggtt cgggcattcc cgccaacgcc
     1321 gacctggtgt ttgacataac cgttttggcc gtccagtagg gttaaaaatg gtgacaaaag
     1381 cacaatatgt agcggtattg acttagcccc aagcactagc tatattaata aggcgaaagt
     1441 cattaacaaa caaaaacgtc ccaggagggg attcggacaa tgaccaaaaa tattactatc
     1501 ttcaccacca acacctgcag ttactgcggc atggtcaaaa gatatctgga catgaagggc
     1561 atgagctatg acgtcgtcaa cctcgacgaa caccccgaac gccaggccga ggtctatgac
     1621 atgagcggcg ccctgaccgt gccaatcaca gtggtcacca agcacgacga ctccaaagaa
     1681 gtggtcgttg gatacaatct ttcacgctta gcccccgccg tagcgtaaac tagttacatg
     1741 cacgacctgg taatagttgg cgccggtccc gcggcgctct ctgcggcgct ttatacctca
     1801 cgcgaggata tagacacgct tttatttgaa aaaggcgcca ttggcggcct ggccgcggtt
     1861 accgactgga tagataatta tccgggattc gccaaaggca tcgcaggttt ggaactatct
     1921 gatgccatgc gtgcccaggc cgagcgcttc ggcgccaaga ttgaactggg agaggtcagc
     1981 aagataacca aagacggcga cctgataaaa ttggatacca caagcggtga aatcaaagcc
     2041 aaggccgttt tgatagccac cggcagcgac taccgcaaga ttggcgtacc cggcgagcag
     2101 gaatactacg cccgcggcgt ccactactgc gccacctgcg acggcgcttt ttatcgtgat
     2161 aagcggctgg tagtggtcgg tggcggcaat tcggcggtcc aggaaagcat gttc
//
LOCUS       DASPTF010000065         5452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1829819, whole genome shotgun sequence.
ACCESSION   DASPTF010000065 DASPTF010000000
VERSION     DASPTF010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829819"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..656
                     /locus_tag="VEH48_02125"
     CDS             357..656
                     /locus_tag="VEH48_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18196.1"
                     /translation="MLDTFITSRVRRKIIVVYAKYPDFKTHVRGLAKLIKEDAGNIQR
                     ELKRLEKIGFLISERQSNTKIYYTNKQFPIFKELQSIVLKSQRAAANKRSGTPQL"
     gene            832..1044
                     /gene="rpsU"
                     /locus_tag="VEH48_02130"
     CDS             832..1044
                     /gene="rpsU"
                     /locus_tag="VEH48_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXY18197.1"
                     /translation="MIQVTRKDAKESTENLLRRFGRKVQQSGVLAEAKQNQYFEKPIS
                     KRDRRTKAILRRQRKAAKIKKLKLEQ"
     gene            1047..1502
                     /locus_tag="VEH48_02135"
     CDS             1047..1502
                     /locus_tag="VEH48_02135"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HXY18198.1"
                     /translation="MAIQQQIDSDIKTAMLAGDKTKTETLRTVKSALLNEAIAAGARE
                     SGLSDEQAQKILAKEAKKRQEAADLYKQGGSPERAEAELAEKAVIDGYLPEQASEADI
                     KAAVEAEIGKLDNPGPQNMGQVIGAVRVQLGAGADGAVIARLVKEALAK"
     gene            1499..2035
                     /locus_tag="VEH48_02140"
     CDS             1499..2035
                     /locus_tag="VEH48_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HXY18199.1"
                     /translation="MIILLGTPGSGKTTQTRMLADYLGCPWFSMGELIRRDITGQHRQ
                     DMLAGKIIDDKVTLDIVDKNLAKIDTSKECVFEGNPRSVPQARWWVQQEKAGRFKIKA
                     VLHLVVDPSVAETRMLKRGRLDDHDENVIETRFKEYYRSIAPILEYLKEDGISIHEID
                     ANSTVEEIAQKIHESLGV"
     gene            2032..2778
                     /gene="map"
                     /locus_tag="VEH48_02145"
     CDS             2032..2778
                     /gene="map"
                     /locus_tag="VEH48_02145"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXY18200.1"
                     /translation="MSVKTPEEIETMREGGRRLARILNGLAAEVKPGVRPTDLSATAR
                     EQIKKAGMQPVVLGYEGFPDVLCVSVNECIVHGVPGKEPFRHGDVVKVDLTLGYKGLI
                     LDSAVTVVAGNSDDPDVKRLINGTKEALAVGIAAIRGDGTRVGDISAAVQDALNKCKL
                     GIIRDLVGHGLGYEIHEEPNIPNYGVAGTGPVLHAGSTIAIEPMASLGDWQIGMLKNG
                     GVIMRDGSLGAHFEHTVLVTENGAEILTQP"
     gene            2830..3444
                     /locus_tag="VEH48_02150"
     CDS             2830..3444
                     /locus_tag="VEH48_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXY18201.1"
                     /translation="MPSRNVLKQDVAGGYYHIYNRGVDKRLIFLERKDYAVFLNLLKR
                     YLSIEAHKDNKGVIYPHFYNQLELLSFCLMPNHLHLLIYQDEPGTMRKLMSGVFTSYS
                     KYFNKTYKRRGPLFESRYKASLISDQIYLEHISRYIHLNPSEWRQYSYSSLPYFLGRL
                     KAEWVRPAKVADLFDSKADYLKFMEDYEGHRQMLEQTKRELANY"
     gene            3569..4102
                     /locus_tag="VEH48_02155"
     CDS             3569..4102
                     /locus_tag="VEH48_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18202.1"
                     /translation="MSEKLSVQEQSTPIAEDLEREDWLKRYREARSATIASKFHENWR
                     RTRLQEDGSYTPRIKETTDKDWIAAHGSNQVDIANTDYFGLPRDWQEENVAAARVIYD
                     LVDNFRTRGMVVGMFENPAPAGTRVHEEWLGRNQWAAGGELDKPFVELPADEQAKDVE
                     QVQIGRREFGYQERRAA"
     gene            4182..4559
                     /locus_tag="VEH48_02160"
     CDS             4182..4559
                     /locus_tag="VEH48_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18203.1"
                     /translation="MSTIEEQTPEKNPLPIYDNGLSPDDRERATKLVNGYIHHAVGKQ
                     PYHEIVQEDLHDPLVAAVRVGLEAHGRHAAKTYEKAATKHYKAHKGEYQVQAVKDIQA
                     AGGGTSFGTERFAAEHGTSVSQK"
     gene            4736..>5452
                     /gene="ftsA"
                     /locus_tag="VEH48_02165"
     CDS             4736..>5452
                     /gene="ftsA"
                     /locus_tag="VEH48_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXY18204.1"
                     /translation="MSEEKLSYYVGLDIGTTAVRCVVGELTPEASTPSIIGFSSSDNT
                     GMRKGNVAHAEEVTQAIINAVQEAERMSGREIKSATVNVNGAHVQGINSRGVIAVSSA
                     DRQITNDDRLRVEEAATVVQLPANKEIIQIFAKNYRLDGQDNIKDPLGMHGVRLEVDT
                     HILLAGTPALKSLDQVLQNAEVRPNHHSVTSLAAAEAVLDRKQKESGVAVIDIGAATT
                     NLIVIEDGEVEHIAVIPMGGT"
BASE COUNT         1553 a         1341 c         1404 g         1154 t
ORIGIN      
        1 gactacccca gaaacggccg taaagccatt gccgaatcta ccgaaattgg cgttccccag
       61 ggagcggaaa cactggctat agaaaccgca ctcgcagaca ttgagcaggt gttggaatct
      121 ttagaacctg ccgctaccga agaagagcta gccgcttaag ctaccgtttt ttcttttagc
      181 cttttggaaa accgtcccgg agtttttgtt actttggcgg tccaaagtaa cagattattt
      241 tgcactttgt aaaaagtgct ggttggcggc tcaaaagtat gaaagttcct ttacaaaaaa
      301 tgtagttgtg ttttatactt agctttgacg ttgttctaat aacaatagag gtgtttatgc
      361 ttgatacgtt catcacgtcc agggtgcggc gcaagattat cgtggtctat gccaagtacc
      421 ccgatttcaa gacccacgtc cgtgggctgg ctaagttaat caaagaagac gccggcaaca
      481 tccagcgcga gctaaagcgc ctggagaaaa tcggcttttt aatcagcgag cgccaaagca
      541 acaccaaaat ttattacacc aacaagcagt ttcctatctt taaggagctg cagagcatag
      601 tgttgaaatc gcaacgggca gccgctaaca agcgctccgg caccccccaa ctctagcaag
      661 taaattgatc taattaacct aatttctaat taacctaagt aatttctaaa tctctaaacc
      721 ccaaacagat tgggtgattt attaattggc gcttaattag tgcaattagg tcaattagag
      781 caattagaaa ttgcttggca tttaacctct gttagagtaa aatatgccaa gatgatacaa
      841 gttaccagga aagacgccaa ggagtctacc gaaaatctgc tgcggcgctt tggccgcaaa
      901 gtccagcagt ccggcgtgct ggccgaggcc aaacagaacc agtattttga gaaacccatc
      961 agcaagcgcg accggcggac caaggctatt ctgcgccgcc agcgcaaagc cgccaaaatc
     1021 aagaaactca agctggagca gtagccgtgg ctattcagca gcagatagac agcgacatca
     1081 aaacagcgat gttggcgggc gataaaacca agaccgagac cctgcgcacg gttaagagcg
     1141 cgttgctgaa cgaggctatc gccgccggag cccgcgaaag cggcctgtct gacgagcaag
     1201 cccagaaaat cctggccaaa gaagccaaaa aacgccaaga agcggctgat ttatataaac
     1261 aaggcggctc gccggagcgg gcagaggccg aactggccga gaaggccgtg atagacggct
     1321 atttgccgga acaagcctct gaagccgaca tcaaggccgc cgtagaggct gagattggca
     1381 aactggacaa ccccgggcca caaaacatgg gtcaggtcat cggcgctgtg cgtgtccagc
     1441 ttggagccgg cgccgatggt gcagttatcg cccgcctggt taaagaagcc cttgccaaat
     1501 gattatattg ttaggtacgc cgggctccgg taagaccact cagacccgaa tgctggctga
     1561 ttatctgggc tgcccttggt tctccatggg agagcttatc cgccgggaca tcacaggtca
     1621 gcaccgccag gatatgctag ccggcaaaat aatcgacgat aaagtgacat tggacatcgt
     1681 tgataagaac ctggcaaaaa tcgatacctc caaagaatgc gtgtttgaag gcaatccgcg
     1741 cagcgtgccc caagcccggt ggtgggtcca gcaggaaaaa gccggccgct tcaaaataaa
     1801 agccgtcctg catctggtag tcgacccgtc tgtggccgaa acgcggatgc tcaagcgcgg
     1861 ccgcttggac gaccatgacg agaatgttat cgaaacccgt ttcaaggaat attaccgctc
     1921 aatcgcccca atcctagagt acctaaaaga ggacggcata tctattcatg agattgatgc
     1981 caacagtaca gttgaagaga tcgcccaaaa aatccacgaa agcctgggag tatgagcgtt
     2041 aagacacccg aagagattga aaccatgcgc gagggcggcc ggcgcctggc gcggattttg
     2101 aacggcctgg cggccgaggt caaacccggc gtgcggccaa ctgatctttc ggccacggcc
     2161 cgcgaacaaa tcaaaaaagc cggcatgcag ccggtggtcc tgggctatga gggcttcccg
     2221 gatgtgctct gcgtatctgt taacgaatgc attgtgcacg gcgtgccggg caaagaaccc
     2281 tttcgccatg gcgacgtggt caaagtggac ttaacgcttg gctataaagg tttgattttg
     2341 gatagcgccg tcaccgtggt ggcgggtaac agcgatgacc cggacgtcaa acgcctgata
     2401 aacggcacca aagaggccct ggccgtcggc atcgccgcta tccgcggcga cggcacccgg
     2461 gtcggcgaca tcagcgccgc cgtccaagac gcgttaaata agtgtaaact cggcatcatc
     2521 cgcgacctgg tcggccacgg cctgggctac gagatccacg aggagccgaa catccccaac
     2581 tacggcgtgg ccggcaccgg cccggtattg cacgccggca gcaccatcgc cattgagccg
     2641 atggcatcgc ttggcgactg gcagatcggc atgctcaaaa acggcggtgt catcatgcgc
     2701 gacggcagcc tgggcgccca cttcgaacac accgtcctgg tcaccgaaaa cggcgccgaa
     2761 atcctcaccc agccataact gcggaagaac gtcctcggaa aataagaatt tgtggtgtaa
     2821 tataaattta tgccaagccg aaacgtcctt aagcaggatg tggcgggtgg gtattaccac
     2881 atttataatc gaggtgttga taaacgcctg attttccttg aacgaaaaga ctatgctgta
     2941 ttcctcaact tattaaaacg atatctttca attgaggcgc ataaagacaa taaaggcgtc
     3001 atatatcctc atttttacaa tcaattagag ttattaagct tttgtttaat gcctaatcac
     3061 ttacaccttt taatttatca agacgagccg ggcaccatgc gcaaactaat gagtggagtt
     3121 tttaccagtt acagcaaata cttcaataaa acatataaac gccgcggtcc gctattcgaa
     3181 agccgataca aagcatcatt aatttctgat caaatatatc ttgagcatat atctcgctac
     3241 atacatctta atccgagcga gtggcgacaa tattcttata gcagcttgcc gtattttttg
     3301 ggcagactca aggccgaatg ggtaaggccc gcgaaggttg cagatttatt tgactccaag
     3361 gctgactatt tgaagtttat ggaagactac gagggacatc gtcaaatgct agagcaaacc
     3421 aagcgcgaac ttgccaacta ttgatctgcg gaggacgttc ttccgcaagc ccagagatac
     3481 ttgcggaaga acgtcctccg caaagcacaa ggggcttgca tttttagcat aagtatgata
     3541 gtattgcact cgtaatccta aaataaaaat gtccgaaaaa ttatcggtcc aagaacaatc
     3601 gacgccaata gccgaagatc ttgaacggga agattggtta aagcgctatc gggaagcccg
     3661 gtctgcaaca atagccagca agtttcatga aaattggcgt agaactagac ttcaagaaga
     3721 tggttcatat actccacgga ttaaagaaac aacagataaa gactggattg ccgcccacgg
     3781 cagcaaccaa gtcgacatag ccaatacaga ctattttggc ttacccagag actggcaaga
     3841 ggaaaatgtc gccgccgcca gggttattta tgacctagtt gataatttta gaacccgcgg
     3901 catggtcgta gggatgtttg aaaatcctgc gcccgcagga acgcgagtgc acgaagagtg
     3961 gctaggccgc aatcaatggg ctgccggtgg agagttggat aagccgtttg ttgaactccc
     4021 ggctgacgaa caagcaaaag atgtcgagca agttcaaatc ggccgccgag aattcggtta
     4081 tcaagagcga cgcgccgcct aagttgactt tttagcataa gtgtgataca ctttattcaa
     4141 gctcacaaaa acaaagagcc gcaagaaata aggaaaatat aatgagcact atagaagaac
     4201 aaactccaga aaaaaatccg ttaccgattt atgataacgg tttatcgccg gacgatagag
     4261 aaagggccac taagctagta aacggctaca tccatcatgc tgttggtaaa cagccttatc
     4321 atgagattgt ccaagaagat ttgcacgatc cactggtcgc agcagttcgt gtcggtttag
     4381 aagcgcatgg gcgtcatgca gctaagacat acgagaaagc tgccactaaa cactacaagg
     4441 cccataaagg agagtatcaa gtccaagccg ttaaagatat acaggcggcg ggcggtggca
     4501 cttcttttgg tactgaaaga ttcgccgctg aacacgggac gtctgttagc caaaagtaaa
     4561 ctccaaaatt gcactactca accgccctga agggcggttt tttcattgat gtattagata
     4621 ttgttaattc attgaaaatt gtaaattgct aattgataat tgctcacttg ctaagaagtg
     4681 agcgttagcg gtatagtggg gtagataagt tatacttata aaataaatat ttagcatgag
     4741 cgaagaaaaa ctaagttatt atgtagggct ggatatcggt accacggccg tgcgctgtgt
     4801 ggttggcgag ctgacaccag aggccagcac ccccagtatc atcggctttt cttcgtccga
     4861 taacaccggt atgcgcaagg gtaacgtggc ccacgccgaa gaagttacgc aggcgattat
     4921 caacgccgtg caggaggccg aaaggatgag cggccgggaa atcaaatccg ccactgtgaa
     4981 cgtcaacggc gcgcacgtgc aaggcatcaa cagccggggc gtgattgccg ttagtagtgc
     5041 cgacaggcag atcaccaacg acgaccgcct gcgggtggaa gaagccgcga cggtggtgca
     5101 gctaccggcc aacaaagaaa ttatccaaat ttttgccaag aattaccggc tggatggcca
     5161 ggacaatatt aaagatccac taggcatgca tggtgtgcgg ctggaagtgg acacgcatat
     5221 tttgctagcc ggcacgccgg cgctcaaaag ccttgatcag gtgctgcaaa acgccgaggt
     5281 gcggcccaac caccacagcg tgaccagcct agccgccgcc gaggcggtac tggaccgcaa
     5341 gcaaaaggag tccggcgtag cggtaattga catcggcgcg gccaccacca acctgatagt
     5401 cattgaggac ggggaagtcg agcatatcgc cgtcatcccc atgggaggta cg
//
LOCUS       DASPTF010000066         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1392798, whole genome shotgun sequence.
ACCESSION   DASPTF010000066 DASPTF010000000
VERSION     DASPTF010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392798"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VEH48_02170"
     CDS             complement(<1..337)
                     /locus_tag="VEH48_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18205.1"
                     /translation="MPRYLRNLRLPLNSEQPQILHIDLNSCFATVEQQARPTLRGRPV
                     GVTNRLTKNACVVAASYEAKALGVKVGMTFSEAKLLAPELIMVETDPPKYHYVYKKLI
                     KIMRSYSPNI"
     gene            complement(294..605)
                     /locus_tag="VEH48_02175"
     CDS             complement(294..605)
                     /locus_tag="VEH48_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18206.1"
                     /translation="MENVNKINKEVSIVAFYFKNSARRLRCFPKRMEYDGKRVDFTET
                     GMVHPTRQGQRMIHIFDMTDGSADYRLEFDAQALSWKLVAITDVADASLSAKLAVAPQ
                     F"
     gene            complement(1067..2563)
                     /locus_tag="VEH48_02180"
     CDS             complement(1067..2563)
                     /locus_tag="VEH48_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18207.1"
                     /translation="MHERHHHHLNEQHHPAIRIPLANRAIVSELLGREIHHTPASSNA
                     EQYPHKHKWDVLFAVMMKARYGHAEDAVREILDLFAGQRDDGLVPSPQFVENLRDIDL
                     ERETFTDRDASEYGQPPIEALAALKVYEGLMIEDKQDQGEEFLLNIYSKLAASYTYFA
                     RDRQHADPRIKLIFNTHPSETGRDSDPTFDFFKVRLPTFGENTPHLIQEANRVTNFFS
                     MIALNEMNRQDGWETQKIRERFEVYDVMFNCMYAENLNHMATLAKELAAIAVREGDET
                     AYLKLSEDAEHWQQLASTVEQQILDLMWFENTNPKNPVKGLFLALKPEGPIEEVSISN
                     LFPLILPNLRQEQLESILFLLDNYFCPKDIVELPIPSVPPINPITNRPNRKYDPYNGE
                     RDTLWRGDTWMNTNWYLVKRGLAMQASRADLQHSPDLIEKCNHYISFISERSYKTVVG
                     VGSWEHYGPEDARPHRTDKSPNFAWSGLAYWLYEDFLAADRDSLALAA"
     gene            complement(2595..2669)
                     /locus_tag="VEH48_02185"
     tRNA            complement(2595..2669)
                     /locus_tag="VEH48_02185"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2634..2636),aa:Glu,seq:ctc)
     gene            complement(2712..>3367)
                     /locus_tag="VEH48_02190"
     CDS             complement(2712..>3367)
                     /locus_tag="VEH48_02190"
                     /inference="COORDINATES: protein motif:HMM:NF037564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HXY18208.1"
                     /translation="IVHVGGSPDALSEVESGGFRNLDEFAYGSYFTRISARDAPHNVY
                     TSFAELDKLNQAKGYTSSSFTSWPRKADKKLATPTAGTIDFAISSPDYYAHYDYDAKT
                     NSYLRSEGGSPHLDLVSATDTTGVQLDPKIVIAIVVPQSLGVLDASGAYYTDYADSGS
                     GAAYIFQDGGVTVGQWSKGGTTDQIKFTDDAGNPIKLNTGQTWITLVGSDGEVSYKP"
BASE COUNT          692 a          877 c          937 g          861 t
ORIGIN      
        1 cgatattggg cgagtaagac ctcatgattt taatcagttt tttgtagacg tagtggtact
       61 tgggcgggtc ggtctcaacc atgattagct ccggggccaa caacttagcc tcggaaaaag
      121 tcatgcccac tttgaccccc agggccttgg cctcgtagct ggcggccacc acgcaggcgt
      181 ttttggtcag gcggttggtg acacctaccg gccggccgcg caatgttggc ctggcctgct
      241 gctccacagt ggcaaagcag ctgttcaggt caatgtgcag gatctgcggc tgctcagaat
      301 tgaggggcaa ccgcaagttt cgcagatagc gaggcatcag ctacatccgt tatggcgacc
      361 agtttccagc ttagcgcctg ggcgtcaaac tccaggcgat agtcggcgct gccgtcggtc
      421 atgtcaaaga tatggatcat ccgctggcct tgcctggtcg ggtgcaccat gccggtctcg
      481 gtgaaatcca cccgcttgcc gtcgtactcc atacgcttgg ggaagcagcg cagccgccgg
      541 gcgctgttct tgaagtagaa tgctacgatg ctaacttctt tattgatttt gttaacgttt
      601 tccatttttt taactcctcc gcaactcacg ccgtccgccc tgctgcctgg cagagccagg
      661 catgccggcg gcctcttggc gcatcacggt gtcagaagct ccgttgctca ctcggtgtac
      721 gtccctgtac acctcgctct gctactcgct tcttccttgt gctgcatcca agcgtgaagt
      781 tgctcatatt tagtctatta aggttataga aagagggcca gccatctgtt aatttgatgt
      841 tggcgaacgg gtttttcgac cgagttgccg ggtaaaaact tctgaccgtt ctcaaaagtc
      901 tcgtaaccgg aaacttattg tgctttgttg agtcggcagt gactgtggtt taccgcccga
      961 gtagtagtct gcgaggagag accttacgga atcctcacac ggctaagccc tagtttacgc
     1021 caagccgctt aagccccgca atattgattt atatgaattt tgctttttaa gctgctagcg
     1081 ccaaggaatc tctatcggcg gccaggaaat cctcgtagag ccaatacgcc agcccgctcc
     1141 aggcaaaatt gggcgattta tccgtacggt gcgggcgggc atcttctggg ccgtagtgct
     1201 cccaagagcc gacaccgaca acggttttat aggaacgctc gctgatgaag gatatgtagt
     1261 gattgcattt ctcaattaag tctggactgt gctgtaaatc cgcgcgggaa gcctgcatgg
     1321 ccaagccccg ctttaccagg taccagttag tgttcatcca ggtatcgccg cgccacagcg
     1381 tgtcccgctc gccgttgtat ggatcatatt tacggttagg cctattagtt atggggttaa
     1441 tgggtggaac actggggatc ggcagctcaa caatatcttt gggacaaaag taattatcta
     1501 gcaggaacaa tatggattcc agctgctctt ggcgcaggtt tggcaagata agcggaaaaa
     1561 ggttgctgat actgacttct tcaatcgggc cttccggttt aagcgctaag aagagccctt
     1621 tgaccgggtt tttggggttg gtattctcaa accacataag gtccaggatc tgctgctcca
     1681 cggtcgaggc caactgttgc cagtgctcgg cgtcctcgga aagtttcagg taggctgttt
     1741 catcaccttc ccggacggcg atggccgcca actctttggc cagggtcgcc atatggttaa
     1801 ggttctcggc gtacatacag ttaaacatca catcgtagac ctcaaagcgc tcgcggattt
     1861 tttgagtctc ccagccgtcc tggcggttca tctcgttcag cgcgatcatg ctaaagaagt
     1921 tagtgacgcg gttggcttcc tgtataagat gtggcgtatt ttcgccgaaa gtcggcagcc
     1981 ggaccttgaa aaaatcgaac gtcgggtcag agtcccggcc ggtctcgctg ggatgggtat
     2041 taaagatcag ctttatgcgg gggtcggcgt gctgcctgtc cctggcgaag tatgtatatg
     2101 acgctgccag tttagagtag atattgagta aaaattcctc tccttgatct tgtttgtcct
     2161 caatcataag accttcgtaa acttttaacg ccgctaaggc ttcaatcggc ggctgcccat
     2221 attcactggc gtccctgtcc gtgaatgtct cgcgctccag gtctatatcg cgcaagtttt
     2281 ctacgaactg tgggcttggt accagaccat cgtccctttg gccagcaaat aggtccagga
     2341 tttctcttac cgcgtcttcg gcatgcccgt aacgggcttt catcataacc gcaaacagga
     2401 cgtcccattt gtgtttatgg gggtactgct cggcattgga agaagccggc gtatggtgga
     2461 tctccctgcc cagaagttcg ctgactatcg ctcggttggc cagcggtatt ctgattgctg
     2521 ggtggtgctg ctcattcaga tgatggtgat ggcgttcgtg cataaaataa aaaagcctgt
     2581 cgcgtgaggc ttaatggtga tcccaagggg agtcgaaccc ctgttgctgg gatgagaacc
     2641 cagtgtcctg accactagac gatgggacca cgaatagacg cgataatttt agcagaacag
     2701 gggtggatag tctatggctt gtagctgact tcaccgtcag agccgacaag ggtaatccaa
     2761 gtctggccgg tgttcaactt gatagggttg ccggcgtcat cggtaaattt gatctggtcg
     2821 gtagtgccgc ccttgctcca ctgcccgacc gtcacgccgc cgtcctgaaa gatgtaagcc
     2881 gcgccgctgc cgctgtcggc gtaatccgtg taataagcgc cgctggcgtc caggactccc
     2941 agcgattgcg gtactactat ggcgatgact atcttagggt ccagctgcac gcccgtcgta
     3001 tccgttgcgc tgacaaggtc aaggtgcggc gacccgccct cactgcgcag atagctgttg
     3061 gtcttggcgt cataatcgta atgggcgtag taatcggggc tggaaatcgc aaaatcgatg
     3121 gttccggcgg tcggagtcgc cagcttctta tcggctttgc gcggccagct ggtgaaactg
     3181 ctggatgtgt agcccttggc ctggttgagc ttgtccagct cggcaaaact ggtgtaaacg
     3241 ttatgtggcg cgtcacgggc gctgatacgt gtgaaatagc tgccataggc gaattcgtcc
     3301 agattcctga aaccaccgct ttcaacttcg ctgagtgcgt ccggactgcc gccgacgtgg
     3361 acgatgc
//
LOCUS       DASPTF010000067         4997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_687740, whole genome shotgun sequence.
ACCESSION   DASPTF010000067 DASPTF010000000
VERSION     DASPTF010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687740"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VEH48_02195"
     CDS             <1..644
                     /locus_tag="VEH48_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HXY18209.1"
                     /translation="GKFEQFEKQFLAHLKSEPAKLARLHIMPTCGTRYYRFQKGKWMQ
                     KYAEDFTPAQKKKIIKALSEGIDRFGYREPKTWGDIIEDRGSQITFSALGQDIVAKLG
                     EEGVRLKEAWDPDVKKKKKLRDYVANLIPEFEVRIGGGTSIDVTKPGIDKAYGMQKLL
                     DLLSISKKDIFFIGDRLEEGGNDYPVKAMGVDCMAIDHWQETALAIEAILQVT"
     gene            complement(630..1790)
                     /locus_tag="VEH48_02200"
     CDS             complement(630..1790)
                     /locus_tag="VEH48_02200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXY18210.1"
                     /translation="MSNKQALVDFESTNSKPRIVIVCDWLIGGGAERVVEQLHKMYPE
                     APIYASYCTNEWRQKLDGKVITGYLQHWPFSRLRKFLPPLRALWFSHLNFDGYDLVIS
                     SSGAEAKGIKTQRKTENGKRQTIHICYCHAPTHYYWSRYDDYLKNPGFGFFDPLARLG
                     LRMLVGPMRRWDYKAAQRPDYLLANSTYTKEQIKKYYGRDSVVVHPPVDIERFASKAE
                     EPRRGFITAGRQTPYKRIDLAVAACTKLNLPLVVLGSGPEHRRLKAMAGKTITFLRGK
                     SDEEVARYFQTSLAFIFPGVDDFGIVAVEALAAGTPVIAYKAGGALDYIQPGKTGEFF
                     NEPDAKSLSTVLQKFNPEKYNHTQIQKSADQFSAANFRSNFQQFVKKCLSNL"
     gene            complement(1795..3240)
                     /locus_tag="VEH48_02205"
     CDS             complement(1795..3240)
                     /locus_tag="VEH48_02205"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXY18211.1"
                     /translation="MKSNGSLAYGLFLIIGDFIALLAAFSGGYILRVSVSHRPISENI
                     HASTYLEIFLILLPFWIMLFGLLGLYSSSIYEKRFTEAARLLIGSFIGLLSVTSLAYF
                     DRRPIFPAHLVPVYGFILAFLFLLIFRNGARALRAKLFDYGIGITNILIVGNTRVARE
                     LVESLANYRTSGYRIVGIVGAKEHTTEKYPHLRIFKTFKEAMAKLKTDDIHSIVQTEL
                     YAAGEANNEVLEFAQAHHIAYRFIPGNSELFVGNIEVELFRSSVPVIAVHQTALIGWG
                     RIAKRLTDLIFGTVFLIIALPFMLLVAILIKVFDFRGPLLHKDLRFTRFGNTASIYKF
                     RSMKQAYSGLTPEEAFTKMGRPELIKRYRQNGDFLADDPRLSRVGRYLRRTSLDELPQ
                     LVNIVNGDISLVGPRALQTAELEKYTNKDLILSVKSGLTGLAQISGRREISFEERRRL
                     DMYYVQNWSIWMDFMIIIKTFRVLFSRIGAR"
     gene            3299..4660
                     /locus_tag="VEH48_02210"
     CDS             3299..4660
                     /locus_tag="VEH48_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXY18212.1"
                     /translation="MKQRVPNLYIQILRWGIGGILVIAPFYAALSIWLASGLHHLDFF
                     KIWKEVALAILGLILLGFVFTHKQYAKLIWRKKLLLLIVAYTALLLIDGFYVIFRSEV
                     SEKAVIYGWLIDIRPVAIFAVALLTFTISRQTNWPPFPWKKLLLIPALIAITFGFLQM
                     TVLPKDILRHIGYGPSTTLSYQTVDNQPNLVRVQSTLRGPDPFGAYIGIILVALVALF
                     IEDKAPKKRLAWGAFFLLSLIVLYGTYSRGAELGLLASLVVLLAIEKRRAFRKHLLVV
                     VGVGVIVLAAGIFAAARGNYLAQNLLFHSSNRSTSAQSSNAQRVQAYKNAVKDIAHHP
                     LGSGVGSAGPASRRNSKGPVKIAENYFLQIGQEVGLLGMAMFIAISIMVAFELWQRRQ
                     ATLAKILLSSLAGITIINMVMHGWADDTLAYVWWGLAGLACAPAILKANKSSNEKTQQ
                     KPS"
     gene            4632..>4997
                     /locus_tag="VEH48_02215"
     CDS             4632..>4997
                     /locus_tag="VEH48_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HXY18213.1"
                     /translation="MKKPNKNRRKLLIQFVEYMVSGGVYFWVGYIILDYLYYAKHWSL
                     WWSTIISNVIGWSVNFILQRFWVFNNKSLKGHQTEVTSRYIFITLVDFVLNYFILYGL
                     KLVGITPAIGQFISSAFFTG"
BASE COUNT         1260 a         1204 c         1290 g         1243 t
ORIGIN      
        1 gcggtaagtt tgaacagttt gagaagcagt tcctcgccca tctaaaatcc gaaccagcca
       61 aattggccag gctgcatatc atgccgacct gcggcacccg ctactaccgc ttccaaaaag
      121 ggaagtggat gcagaaatat gccgaggatt tcacgccggc gcaaaagaaa aagattatca
      181 aggcactgag cgaaggcatt gaccgctttg gttaccgcga gccaaaaacc tggggcgata
      241 tcatagaaga ccgcggcagc cagataactt tttcggcgtt aggacaggac atcgtagcca
      301 agctgggcga ggaaggcgtg cggcttaagg aagcgtggga tccagacgtt aagaaaaaga
      361 aaaaactacg cgattatgtc gcaaatctta tcccggagtt tgaagtaaga atcggcggcg
      421 gcacgtccat agacgtcact aaaccgggaa tcgacaaagc ctacgggatg cagaaattac
      481 ttgacttgtt aagtatttct aaaaaggata ttttttttat cggcgaccgt ctggaagagg
      541 gcggcaatga ctacccggtc aaagccatgg gcgtagactg catggccatc gaccactggc
      601 aagaaaccgc cctggccatc gaagccattc tacaggttac ttaagcactt tttcacaaat
      661 tgttggaagt ttgagcggaa gttggcggcg gaaaattggt cagctgattt ctggatttga
      721 gtatggttat atttttccgg attgaatttt tgtaggaccg tggaaaggct cttggcatcc
      781 ggctcgttaa aaaattcgcc agttttgccg ggctggatat agtccagggc gccgccggct
      841 ttgtaggcga tgaccggtgt gccggcggct aaagcctcca ccgccacgat gccgaaatcg
      901 tcaacgccgg ggaagataaa agccagtgaa gtttgaaagt atcgagcgac ttcctcgtcg
      961 cttttgccgc gtaggaaggt aattgttttg ccggccatgg cttttagtcg gcggtgttct
     1021 ggtccactac ccagcacgac taacggcagg tttagctttg tgcaagccgc tactgctagg
     1081 tcgatacgtt tatacggcgt ctggcggcca gcggttataa aaccgcgacg cggttcttcg
     1141 gcctttgaag cgaacctttc aatgtcaacg ggaggatgga caaccaccga atcccgaccg
     1201 tagtactttt ttatctgttc tttagtgtaa gttgagttag cgagtaaata atcggggcgc
     1261 tgagcggctt tgtaatccca gcggcgcatc gggccgacca gcatccttaa accaagtctg
     1321 gccagcggat caaaaaagcc gaagcccggg tttttaagat agtcgtcgta gcggctccag
     1381 taatagtgcg tcggcgcgtg gcagtaacaa atatggatcg tttgccgttt gccgttttcc
     1441 gttttccgtt gggttttgat gcctttggct tcggcgccgc tggaagaaat gactaaatca
     1501 tatccgtcga aattcaagtg tgaaaaccac aaggcccgca acggcggcaa aaatttgcgc
     1561 aagcgggaaa acggccagtg ctgcaggtaa ccggtaataa ctttgccgtc caatttttgc
     1621 cgccattcgt tagtgcagta agacgcataa atcggcgcct ctggatacat tttatgcagc
     1681 tgctcaacta cccgctcggc gccgccgcct atcagccaat cgcaaacgat tactattctc
     1741 ggctttgaat tcgtactttc aaaatccact aaagcctgct tattactcac cgggttatct
     1801 ggctccaatg cggctgaata agactctgaa ggtttttatg atgatcataa aatccatcca
     1861 gatactccag ttctgtacgt agtacatgtc cagcctgcgg cgttcctcaa agcttatttc
     1921 gcgccgaccg gatatttgcg ccaaaccagt caatccggat tttacagaca gaatcaggtc
     1981 tttgttggta tatttttcca gctcggccgt ttgcaaagcc cgtggcccga ccaggctgat
     2041 gtcgccattt acgatgttga ccagttgtgg cagttcatcc aaacttgttc ggcgcaagta
     2101 tctgccgaca cggcttagac gcggatcgtc ggccaaaaaa tcgccatttt ggcggtagcg
     2161 ctttataagc tccggtcgcc ccatcttagt aaaagcctct tccggcgtga gaccgctgta
     2221 ggcctgtttc atactgcgga atttataaat actggctgta ttaccgaagc gtgtgaagcg
     2281 cagatctttg tgcagcagcg gtccgcgaaa atcaaaaact ttaatgagta tcgcaaccag
     2341 cagcataaag ggcaaagcaa tgatcaggaa aacagtaccg aaaattaagt cggtcagacg
     2401 cttagcgatc cgaccccagc cgatcagcgc tgtctggtgt acggcgatca ccggcacgga
     2461 agagcggaac agctctacct ctatgttacc gacgaacagt tcggaattgc cggggatgaa
     2521 gcggtaagcg atatggtggg cttgggcaaa ttccagcact tcgttattgg cttcgccagc
     2581 ggcataaagc tcagtttgaa cgatgctatg gatatcgtct gtcttcagtt tcgccatagc
     2641 ctctttgaaa gttttgaata tccgcaaatg cggatatttt tctgtggtat gctctttagc
     2701 gccgactata cccacaattc tgtaaccgga tgtccggtaa ttggccagtg attcaaccaa
     2761 ctcacgggcc acccgggtat tgccaacaat cagaatattg gtgatgccga tgccgtaatc
     2821 gaagagtttg gcgcgcaaag cccgggcgcc attgcgaaag atcagcaaaa acaagaaagc
     2881 caggataaag ccgtaaaccg gtaccaaatg ggcggggaaa atcggccggc ggtcaaaata
     2941 ggccaggctg gtgaccgata acaggccgat gaacgaaccg atcagcaagc gggcggcttc
     3001 ggtaaaacgc ttctcgtaaa tggagctgct atacaggcca agcagcccaa agagcatgat
     3061 ccaaaacggc agcaaaatta gaaatatttc aagataggtg ctggcatgga tgttctcgct
     3121 tatggggcgg tggcttacag aaacccgcaa gatgtacccg ccgctaaaag ctgccaacag
     3181 cgcgatgaag tccccgatta ttaaaaataa gccgtaggcc agggaaccat tacttttcat
     3241 aaagacacat tcactatatc atcttattat atgcttgatg ggcgataacg aggggtcagt
     3301 gaaacaaaga gtcccaaatc tgtacatcca aatattacgc tggggtatcg gcggtatttt
     3361 ggtgatagcg ccgttttacg cggctctatc aatctggctg gccagcggac tacaccactt
     3421 ggatttcttt aagatatgga aagaagtcgc actggccatt ctgggcctta tacttctggg
     3481 ttttgttttt acccataaac aatacgccaa attaatctgg cgtaaaaaac ttttactgct
     3541 gattgtcgct tatactgcgc tgttgttaat cgacggattt tatgtaatct tcaggagtga
     3601 agtatcggaa aaggcggtga tctacggctg gctaattgac atccggccgg tagcgatttt
     3661 tgcagtggcg ctactgacat tcactatcag tcgccagact aactggccgc cgttcccctg
     3721 gaagaagctg ctgctgatac cggccctgat agccataact tttggctttc ttcagatgac
     3781 agttttgcca aaagatattt tgcggcacat cggttacggg cccagtacga cgttgtctta
     3841 ccaaaccgtg gataatcaac ccaacttagt gcgtgtgcag tcaactttgc gcggcccgga
     3901 tccgttcggg gcgtacatag gaataatact ggtcgcgctg gttgcccttt ttatagagga
     3961 taaggcgcca aagaaaagat tggcttgggg ggcctttttt ctgttgtcgt taatagtcct
     4021 gtatgggacg tattcacggg gagcagaact tggcctgctc gcatcgctgg tggtgttgct
     4081 agccattgaa aaaaggcggg cttttagaaa acacctgctg gttgtggttg gggttggagt
     4141 gatagtattg gccgccggta tatttgccgc ggcccgcggt aattaccttg cccagaactt
     4201 gctgttccat tccagtaacc gctcaacttc cgcgcaaagc tctaacgccc aaagggtcca
     4261 agcctataaa aacgcggtta aagatatagc tcatcacccg cttggatcgg gtgtgggcag
     4321 cgccggtccg gcttctcgcc gcaacagcaa aggaccggta aaaattgccg agaattattt
     4381 tttgcaaatc ggccaggagg ttggcctttt ggggatggcg atgtttatcg ccattagcat
     4441 catggtcgcc tttgaacttt ggcagcggcg acaggctacg ctggctaaaa ttttgctatc
     4501 cagcttagcc ggtattacga tcatcaacat ggtcatgcac ggttgggccg atgacacact
     4561 cgcctatgtc tggtggggtt tggccggcct ggcttgcgcg cctgctatac taaaagcgaa
     4621 caaaagctca aatgaaaaaa cccaacaaaa accgtcgtaa gctgctcatc caattcgttg
     4681 aatacatggt tagcggcggc gtatattttt gggtcggcta cataatcctg gattaccttt
     4741 attatgccaa gcactggagc ctgtggtggt cgaccattat cagcaatgtg atcggctgga
     4801 gcgtcaactt catcctgcaa aggttctggg tgttcaataa caaatcactc aaaggccacc
     4861 agaccgaggt caccagccgc tacatcttca taacgctggt cgatttcgta ctgaactatt
     4921 ttattttgta cggccttaaa ctggtcggta tcacaccggc catcggccag tttatcagtt
     4981 cggccttttt cactggc
//
LOCUS       DASPTF010000068         2909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_106740, whole genome shotgun sequence.
ACCESSION   DASPTF010000068 DASPTF010000000
VERSION     DASPTF010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106740"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..951)
                     /gene="thyA"
                     /locus_tag="VEH48_02220"
     CDS             complement(34..951)
                     /gene="thyA"
                     /locus_tag="VEH48_02220"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: protein motif:HMM:NF002496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HXY18214.1"
                     /translation="MRQYLELLQDIKDNGVKKSDRTGVGTISVFGRQLRFDLSQGFPA
                     VTTKKLFTRGVVHELIWFLQGSSNIKYLVDNNVHIWDEWPYKHHLMEQGKKIPNTSSE
                     EWKSGIKDFTEKIKNDNAFAKKWGELGPVYGYQWRSWPTPDGKHIDQIEKATEAIKNN
                     PDSRRIIVSAWNVADIDEMAKAGLPPCHLLFQFYVAEGKLSCQMYQRSADTFLGVPFN
                     IASYSLLTMMVAQVTGLKVGDFVHTFGDTHIYLNHLDQVDEQLSRKPRPLPKMKINPK
                     VKNISDFKFEDFELVNYDPYPAIYAPIAV"
     gene            1042..1551
                     /locus_tag="VEH48_02225"
     CDS             1042..1551
                     /locus_tag="VEH48_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18215.1"
                     /translation="MELLNKTEQWLANLYRGAPKLSSGSKKSIVGIWPIVALIFGVLQ
                     LLAVIELWHLGHEVTRVANLVNSFGAYYATNVAVHLNVFYWLSMIVVAADGIILLAAY
                     PALRRQQKSGWNLLFYASLLNLVYGVFSAFNNYGGVASLILQVIVSAIVLYFLFQIRD
                     QYSSVKAPR"
     gene            1672..>2909
                     /locus_tag="VEH48_02230"
     CDS             1672..>2909
                     /locus_tag="VEH48_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXY18216.1"
                     /translation="MDDFSAELKKAGFAGEIDNSEESRQFYSHDASMFEIIPELIVAP
                     AGAKDVERLVKLVAERKRSQPGLSLTARSAGTDMSGAAINDSIIVDFTKHFTKIEAVS
                     EHAAQSQPGVFYRDFEVQTLKHKALLPPYPASRDLCTIGGMVNNNSGGEKSLEFGKTE
                     DFVTRLDVVLADGKSYVLKALNKAELDAKRKQSGFEGEIYRKMFDLLDGNYDEIKLAK
                     PHVTKNSTGYNLWDVWDRQSGIFDLTKLLVGAQGTLGLVTDSHFKLVPARPHSGLLVM
                     FLRDIDALGDIIPKVLESKPATFESFDDATLKLAIRFMPSFLKMLGWKKFIHLLFSLI
                     PEGFQLLRGMPKLVLMVEFNGQTEDEVRQKIKALHRQLNQHRARYEINGFEEDATEGK
                     SEKFWIMRRQSFNLLRSKVK"
BASE COUNT          704 a          742 c          736 g          727 t
ORIGIN      
        1 cgtggcaacg ataatagaga ccataacccg cgcctaaacc gctatcggag cataaattgc
       61 cggataaggg tcgtaattaa caagttcaaa atcctcaaac ttaaaatcag aaatgttttt
      121 aactttgggg ttgattttca ttttgggaag tggacgcggc ttgcgcgata gttgttcatc
      181 aacttggtcc aagtggttaa ggtaaatatg ggtatcgcca aacgtatgga cgaaatcgcc
      241 aactttcaat ccggtaacct gggcgaccat catggtcagt agtgaatatg acgcgatgtt
      301 gaaaggtacg cccaaaaaag tatcggcgct gcgttggtac atttggcagc tgagcttacc
      361 ttcggccacg taaaattgga acaataggtg gcatggcggc aggccggcct tggccatttc
      421 gtcaatgtca gcaacgttcc aagccgaaac gatgatccgg cgcgaatccg ggttattctt
      481 gatagcttca gtggcttttt caatctggtc gatatgcttg ccgtcaggtg tcggccagct
      541 acgccactga taaccgtaga ccggaccgag ttcaccccat tttttggcaa aagcgttatc
      601 gtttttaatt ttttcggtaa agtccttaat gccgcttttc cattcttcgc tactggtgtt
      661 cggtattttc ttaccttgtt ccatcaggtg atgtttgtat ggccactcgt cccagatgtg
      721 aacgttgtta tctactaagt acttaatgtt gctgctgcct tgcaggaacc agattaactc
      781 gtgaacaacg ccacgggtaa atagtttttt ggtggtgacc gccgggaaac cctgactaag
      841 gtcaaacctt aattgccgac caaaaacgct aatggtcccg actccagtac ggtcgctttt
      901 cttgacgccg ttgtccttga tatcctgcaa taactctaag tactgtctca tagccttcta
      961 atattaacat gtgccatctg gcgtatttgc tctcaaccaa ttcttggatt atgcttaaag
     1021 gtataaactg aaggagagtc catggagtta ttgaataaga ccgagcagtg gctggccaac
     1081 ctttacaggg gcgcgccaaa acttagttct ggctccaaaa aatcaatcgt cggtatctgg
     1141 ccgatcgtcg ctttgatatt cggagtactg cagctactgg cggttatcga gctctggcat
     1201 ctcggacacg aggtcacccg ggtcgccaat ctggtaaatt ccttcggagc ttattacgcc
     1261 actaacgtag cggtccatct gaacgtcttt tactggttga gcatgatcgt ggtcgccgcg
     1321 gacggcatca tcctgctggc ggcttacccg gcgcttagga gacagcaaaa atccggctgg
     1381 aacctgttat tttacgcttc tctactgaat ctggtttatg gtgttttctc ggctttcaac
     1441 aattacggcg gtgtggcttc gctgattctg caggtaattg tcagtgccat agtgctgtat
     1501 tttctctttc agatccgcga ccaatactcc agcgtcaaag ctccacgcta ataacttgcg
     1561 caccggcccc tcaggggtta agcttaagca tgatggatcc tgttagagct aagtatttta
     1621 tcaaagataa aatctgccgg ttcgctggca gcgagccgga gctctaacag gatggacgac
     1681 ttcagcgccg agcttaagaa agccggattt gccggcgaga tagataattc agaagaatcg
     1741 cgtcagttct acagccatga cgccagtatg tttgaaatca tcccggagct gatagtcgcg
     1801 ccagcaggcg ccaaagacgt cgagcggctg gtgaagctgg tagctgagcg taagcgctcc
     1861 cagcctggcc tgtcgctgac cgcccgcagc gccggcaccg acatgagcgg cgcggccatc
     1921 aatgattcaa taatcgttga tttcactaag cattttacga agatagaagc cgtcagtgag
     1981 cacgccgccc agtcccagcc cggagtgttt taccgcgact ttgaagtaca gaccctcaag
     2041 cacaaagcgc tgctgccgcc ctacccggcc agccgcgatc tgtgcaccat tggcggtatg
     2101 gtcaataaca attctggtgg cgaaaaaagc ctggaattcg gcaagaccga agacttcgtc
     2161 acccgcttgg acgtcgtgct ggcggacggc aagtcctacg tcctcaaagc cctcaataag
     2221 gccgagcttg atgccaaacg caaacagagc ggctttgagg gcgaaatcta ccgcaaaatg
     2281 tttgacctgc tggatggcaa ttatgacgag atcaagctgg ccaagccgca cgtcaccaaa
     2341 aactccaccg gttataatct ctgggacgtc tgggaccgcc aaagcggtat ttttgacttg
     2401 accaaactgc tggtcggtgc ccagggcacg ctgggcctgg tgaccgacag ccactttaaa
     2461 ctggtaccgg cccggccgca ttccggcctg ctggtaatgt ttttgcgcga catcgacgct
     2521 ctgggtgata tcatccccaa ggttttggaa tccaagccgg cgacgtttga aagttttgac
     2581 gacgccacac tcaagttggc catccgcttc atgcccagtt tcttaaagat gctgggctgg
     2641 aaaaaattca tccacctgct ttttagcttg ataccggaag gcttccagct gctgcgcggt
     2701 atgcccaagc tggttttaat ggttgaattc aacggtcaga ccgaagatga ggtccgccag
     2761 aagatcaaag ccctgcaccg ccagctcaat cagcaccggg cgcgctacga gatcaacggc
     2821 tttgaagagg atgctaccga aggcaaaagc gagaaattct ggataatgcg ccgccagagt
     2881 tttaacttgc tgcgctccaa agtcaagga
//
LOCUS       DASPTF010000069         4566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_536174, whole genome shotgun sequence.
ACCESSION   DASPTF010000069 DASPTF010000000
VERSION     DASPTF010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536174"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VEH48_02235"
     CDS             <1..424
                     /locus_tag="VEH48_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18217.1"
                     /translation="PKKIKPKLFSVTEKSHDILYRANTVFPFTLFPNTVTLDREKLTF
                     ATRYFFRSAKITSVPVRDILSVEANVGLLFGSVHTASRYFITNPYTVNYLWRGDAVNL
                     QRLLQGYIIAHEQQIDCSSIERNQLVAMLEDLGKGASD"
     gene            complement(576..1700)
                     /locus_tag="VEH48_02240"
     CDS             complement(576..1700)
                     /locus_tag="VEH48_02240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXY18218.1"
                     /translation="MIKKFRRSRLGKKANKQARKVKDLVPAGVAEKAAEYNPLAPDPE
                     PTVVTEVPQITSESIAKHREQVLSGARKYIYPLQHSKHRIIIVTVSIVAAAVIGLLTY
                     CSIGLYRLYQYNTFIYRITQVVPFPIAKVGSHYVDYENYLFELRHYVHYYQSQLQLDF
                     SGEGKQQLISFRQQALNDAINGAYIKILAAQNHVSVSGKEVNDRITEVREQNRLGDNN
                     KVFDDVLRNYWGWSENDFKRALKDEILSEKVVAKLDTATTARAAAALAQLKAGGDFQA
                     LAKQVSDALDKASGGDYGFGITKSNPNVPPQVVEVLFQLKAGQTSGVIDAGQTLEIVQ
                     VVNTDGTTVTARHISFNLQDISVFIKPLKTKYPTHTYVHF"
     gene            complement(1693..2286)
                     /locus_tag="VEH48_02245"
     CDS             complement(1693..2286)
                     /locus_tag="VEH48_02245"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXY18219.1"
                     /translation="MSEKHGDQDEPQPAEAPAKKRHFKEQFEDEEALLVFRKHPIVMR
                     KGLIIAMLAILAGTIPALIKPTYGYFLGGLAGGFALGVILIFPSWVRWYFSIYIMTDK
                     RFIQQTRSLLQVNVVDIGLEQIQMINYQISGLQETLLNFGTIVVQTYVGDLVIRDVHR
                     PARIQKKMVHILRDLGIHPTQRPYSGQDSEGKIISDD"
     gene            2358..2433
                     /locus_tag="VEH48_02250"
     tRNA            2358..2433
                     /locus_tag="VEH48_02250"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2391..2393,aa:Arg,seq:cct)
     gene            2451..2789
                     /locus_tag="VEH48_02255"
     CDS             2451..2789
                     /locus_tag="VEH48_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HXY18220.1"
                     /translation="MENILIVTTTTNDIPGYEVVEVYGEVFGLIVRSRNMFSNIGASL
                     RTVFGGEVGGYTKLLIDTREQAIGRLREAAAAKGGNAVLAMRFDTSEIGGIMNEVAAY
                     GTAVKIRPKK"
     gene            complement(2793..3341)
                     /locus_tag="VEH48_02260"
     CDS             complement(2793..3341)
                     /locus_tag="VEH48_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXY18221.1"
                     /translation="MERTLVLLKPDAVQRRLVGDIISRFEKAGLELVATKMVRPDEEL
                     AHKHYPTSRRDFIEGMGNKTLGDYKLSGLNPVDDFGSDDPYKIGLQVQKWLVDFLTSG
                     PVMALVIEGRGAIAQVRKLAGNTIPVLAEKGTIRGDHSDDSPSKANAEKRAIKNLVHA
                     SGDKAEADFEINLWFTPDELKA"
     gene            3442..4014
                     /locus_tag="VEH48_02265"
     CDS             3442..4014
                     /locus_tag="VEH48_02265"
                     /inference="COORDINATES: protein motif:HMM:NF044910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18222.1"
                     /translation="MDKKNSTSAITDNILRILAVSGVAATALVAPNLLIALDKPLRKY
                     LGRLDERARQRELSRVINYMRSQGLIKDDYRHGLRITAKGRRRMQRVNFAKLRVPKPE
                     QWDQRWRLVFFDIPEERKTGRDALIRKLKQLDFYQLQRSVWVHPFPCRPIIEKVADAY
                     QVDKFVTYVETTYIDNQAELAKIFHTILSS"
     gene            4089..>4566
                     /locus_tag="VEH48_02270"
     CDS             4089..>4566
                     /locus_tag="VEH48_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXY18223.1"
                     /translation="MLYEKAVEQFLEYLELEQNRSAKTIQNYSHYLTRLSDFAGEIKL
                     SDIDPELIRKWRLWLNRLGTNTSDELGKTTQNYHLIALRSFLKYCAKRGYDAMAADKV
                     ELARTRRPQVTFLTPEELERLAAQPNTAAQNGLRDRAIIELLFSSGLRVSELVGLDR"
BASE COUNT         1050 a         1175 c         1223 g         1118 t
ORIGIN      
        1 gcccaaaaaa attaagccga agctgttctc ggtgaccgaa aaatcccatg acatacttta
       61 tagagccaac accgtcttcc ccttcacgct tttccccaac accgtcaccc tggaccggga
      121 aaaactgacc tttgccaccc gttatttttt ccgatcggcc aagattacca gcgtgccggt
      181 cagggacatc ctgagcgtag aggccaacgt cggcctgctc ttcggctcgg ttcataccgc
      241 ctcacgctac ttcattacca acccttatac tgtcaactat ctatggcgcg gcgatgccgt
      301 gaatctgcaa aggctgctgc agggttacat tattgcccac gaacagcaga ttgactgctc
      361 aagcattgag agaaaccaat tagtggccat gctggaagac ctcggcaaag gcgcctccga
      421 ttaaaacact tgactagtca agtattttag aaattgacct aattgaccta atttctaatt
      481 tatctaagca atgcctaagt accaaatccc aagctgtttg ggcatttaga tttgggattt
      541 atttagtaat tagtactatt gggtcaatta ggaatttaga agtggacgta ggtgtgggtc
      601 gggtatttgg ttttcagcgg tttgataaag actgagatat cctgcaggtt gaaagagatg
      661 tggcgggcag tgacagtcgt gccgtcggta ttgaccactt ggacgatttc caaggtctgg
      721 ccggcatcaa tcacccctga agtctggccg gctttaagct gaaacagcac ctctaccacc
      781 tgcggcggca cattgggatt ggatttggtg atgccaaagc cgtaatcgcc gccgctggct
      841 ttgtccagcg cgtcggaaac ctgcttagcc agcgcttgga aatcgccgcc ggctttcagc
      901 tgggccagcg cggcggcggc gcgggcggtg gtggcggtat ccagtttggc tacgaccttc
      961 tcggacaaga tctcatcttt tagggcccgc ttgaaatcgt tttccgacca gccccaataa
     1021 ttgcgcagga cgtcatcaaa cactttgtta ttgtcgccca ggcggttttg ctcgcggact
     1081 tcggtgatac ggtcatttac ttctttacca gagacgctga cgtggttttg cgccgccagg
     1141 atcttgatgt aggcgccgtt gatagcgtcg tttaaggcct gctgacggaa acttattagc
     1201 tgctgtttgc cttcgccgga aaaatccagc tgcagctggc tttggtaata atggacgtag
     1261 tggcgcagtt caaacaggta gttttcgtag tccacgtagt gactgccgac cttggcgatc
     1321 gggaacggca ccacctgggt gatacggtaa atgaaggtgt tgtactgata aagccggtac
     1381 aggccgatgg aacaataagt cagcaaaccg atgaccgccg ccgccacgat actgacggtg
     1441 acgatgatga tgcggtgctt agagtgctgc agcgggtaga tgtatttgcg ggcgccggac
     1501 agtacctgct cgcggtgctt agcgatgctt tccgaggtga tttgcggcac ttcggtcaca
     1561 accgtcggct cggggtccgg cgccagcgga ttatactcgg cggctttttc agccacgccg
     1621 gccggtacca ggtctttgac cttccgggct tgcttattgg cttttttgcc gagtctgctg
     1681 cgtctaaatt tcttaatcat cagatatgat tttgccttcg ctgtcctggc cagagtatgg
     1741 ccgctgagtg ggatggatgc ccaaatccct cagtatgtgc accatctttt tttggatacg
     1801 ggccggacgg tgcacgtcgc ggatcaccaa atcgccaacg taggtctgta caactattgt
     1861 accaaagttg agcagcgtct cctgcaggcc ggagatctgg tagttgatca tctgtatctg
     1921 ctccaggccg atatccacca cattgacttg aagcaggctg cgggtctgct ggataaaacg
     1981 cttgtcggtc atgatgtaga tactgaagta ccagcgcacc caggagggga aaatcagaat
     2041 gacgcccaag gcgaagccgc cggccagacc gcccagaaaa tagccgtaag tcggtttgat
     2101 cagcgccggg atggtgccgg ccaatatagc cagcatggcg atgatcaggc ctttgcgcat
     2161 gacaatcggg tgcttgcgga acaccagcag agcctcctcg tcctcaaact gctccttaaa
     2221 gtggcgcttt ttggccggag cctcagcggg ctggggttcg tcttggtccc cgtgcttctc
     2281 gctcatgctc acattatata gggagtagcg agtagtgggt agggagtagt gctcataggt
     2341 gttaaaattg gttcagaggc ccggtagctc agcggataga gcgccgccgt cctaaggcgg
     2401 gcgtcgcagg ttcgaatcct gcccgggtca ccactaataa aggaggtttt atggaaaata
     2461 ttttaatagt aacgaccacc accaacgaca ttccggggta cgaggtggtg gaggtttacg
     2521 gtgaggtttt cggactgatt gtccgcagcc gcaacatgtt tagcaacatc ggcgcttccc
     2581 tgcgcaccgt ctttggtggt gaagtcggcg gctataccaa actgctgatt gatacgcgcg
     2641 agcaggctat cgggcggttg cgtgaagcgg ccgccgccaa aggcggcaac gccgttttgg
     2701 ccatgcgctt tgatacctcg gaaatcggcg gcatcatgaa cgaagtcgca gcttacggca
     2761 ccgccgtcaa aatccgcccc aagaaataat ttttaagctt tcagttcgtc gggggtaaac
     2821 cacaggttaa tttcaaagtc agcttcggct ttgtcgccag aggcgtgtac caggttttta
     2881 atcgcccgtt tctcagcatt ggctttggag ggcgaatcgt cggaatggtc acctctgata
     2941 gtgcctttct ccgctaaaac cggtatggtg ttgccggcca gtttgcgtac ctgggcaatc
     3001 gcacccctac cttcaatgac caacgccatc accggcccgg aagtcagaaa atccaccagc
     3061 cacttttgga cttgcaggcc gattttatag gggtcgtcac taccaaaatc gtctaccggg
     3121 ttcagcccgc tgagtttgta atcgcccagt gtcttgttgc ccatgccctc aataaaatcg
     3181 cgacggctgg ttggatagtg cttgtgggcc agttcttcgt cgggcctgac cattttggtt
     3241 gccaccaatt ccaggccggc tttttcaaaa cggctaatga tatcgccaac caaccggcgc
     3301 tgcaccgcgt ccggcttcaa taaaaccagt gttctttcca ttactgcgac cattataaca
     3361 gatgttatca tcaaaataaa ttgaacacaa gctttattca atcaaatttt aattttttac
     3421 gcgatcgcgt aaaatgtttt tatggacaaa aagaattcta cgtcagcgat aacggacaat
     3481 atacttagga tcttagctgt tagcggagtg gcggccaccg ctctggtcgc tccaaattta
     3541 cttatcgcac tagataagcc gttgcgtaaa tatctgggcc ggctagatga gcgcgctagg
     3601 caacgcgagt tgagcagagt aattaactat atgcgctctc agggattaat taaggatgat
     3661 taccgccacg gtttacgaat caccgccaaa ggccgccgcc ggatgcagcg tgtcaacttt
     3721 gccaaactgc gcgtgcccaa gccggagcaa tgggatcagc gctggcgctt agtgtttttt
     3781 gacatcccag aggaacgaaa aaccggtcgc gacgcgctga ttcgcaaact taaacaactg
     3841 gatttttatc agctgcagcg cagcgtttgg gttcatccct ttccctgccg gccaatcatt
     3901 gagaaagtgg ccgatgccta tcaagtggat aaattcgtaa cctatgttga aacgacttac
     3961 attgacaacc aagccgaact ggctaaaatc ttccacacga ttttaagctc gtaaaacttt
     4021 tgcgcgatcg cgtaaaagtt ttacgagatt gtacgacgtg gggttttggc gtttgctaca
     4081 ataaagttat gctttatgaa aaagcagtag agcaattttt ggaatatctg gagctggaac
     4141 agaaccgcag cgccaaaacc attcaaaatt acagccatta cctgacccgt ttgagcgact
     4201 tcgccggtga gatcaaactg tccgatattg atcccgagct gatccgcaag tggcggctgt
     4261 ggctcaaccg gttgggcacc aacacttcgg acgagctggg caagactacc cagaactacc
     4321 atttgattgc cctgcgcagt ttcctaaaat attgtgcgaa gcggggttat gacgcgatgg
     4381 cggctgataa ggtcgagttg gcgcggacgc gccggccgca ggtgaccttt ttgacgccgg
     4441 aagagctaga gcggctggcg gcccagccca acaccgccgc ccagaacggc ttgcgcgacc
     4501 gcgccatcat agagctgcta ttcagcagcg gcctgcgggt cagcgagctg gtcggtctgg
     4561 accgcg
//
LOCUS       DASPTF010000070         4758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1972685, whole genome shotgun sequence.
ACCESSION   DASPTF010000070 DASPTF010000000
VERSION     DASPTF010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1972685"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VEH48_02275"
     CDS             complement(<1..1232)
                     /locus_tag="VEH48_02275"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HXY18224.1"
                     /translation="MKEIAKKTVAAVLGFQIRRLYKKNDFKAVGVVGSIGKTSTKLAI
                     ATVLDKKFKAQHQEGNYNHIVTAPLVFFGEKTPSLFNPFAWLGVFWRNEKHLRRPYPY
                     EIVVLELGTDGPGQIAEFQKYLKLEVAVVTSIVPEHMEFFGTLDEVAAEELSVSKLSG
                     LVLANADLCDKKYLDSLPDILTYAIRTNADYRLVNDKNIAVLSSGQTQLARFDSSNKT
                     LPELYSMLAAAAVAVKFGLEPEEIKNGLHAISPAPGRLQRLAGINSSVILDDTYNASP
                     AAVKLALSSLYQMESPQKIAILGNMNELGPSSEEEHRKIGEFCDPKHLSMVLTLGPDA
                     NKYLAPAAETRGCKVITFDNPYTVGDYLKPVIKPGAIVLAKGSQNGVFAEEALKVLLA
                     NPKDQTKLVRQSPAWLKI"
     gene            complement(1256..3019)
                     /locus_tag="VEH48_02280"
     CDS             complement(1256..3019)
                     /locus_tag="VEH48_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18225.1"
                     /translation="MEPLPGQSFNPTQPTPEPAASEQPAPPAPSVMPGSMTAYPNPPS
                     PNPTILFQSDQPAAPDEPKGFFGRLRRKAVVIPVLAVLLIGGGGAAAYFGYYMPNKPA
                     NIWASALANTGKGYDALSKYAVSQVGSKNKGIKLSGSFRTTGSFASDGSIQGSNDGTN
                     GEFTGSVSAVGVKVSFDTRLVRSSVGTPDVYFKIDGLQGLGTLLGGSDSPYASVLNGI
                     NGKWYFIDHTLFDQYTGSATGGLQITSSDVSSILNAVGNAGDQNVFTKDPTKMAFTVS
                     QNVGKEKQDGRSVYHFKVSVNKQNFKSFVTSLCSNLENSSLKKFFANDSQNVASAIGC
                     DNVTSVVNSLNTTRTADVWVDLHTKLVHKIRFTDKNNSTNYFEIGQDYQGGSSYPFSI
                     GFHDKSGSDVSSGSLGITVNTQTNSVTVNGSVQDGNNFTGSLNLTVAPNNSAVNVQAP
                     AGAENIIQLLNDAGFSNLLGGGSSVNDTKRMTDINALQGQVEAYNTNTGYYPTLADLN
                     NASWRSQNLQGLDPSALQDPAGNSQTLAASPAAHVYAYQPTPAGCNNTTIDCSGYTLA
                     ATLDDGTPYVKTGSDLTTLTN"
     gene            complement(3044..3736)
                     /locus_tag="VEH48_02285"
     CDS             complement(3044..3736)
                     /locus_tag="VEH48_02285"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXY18226.1"
                     /translation="MRLNVFLAKSGVASRRGADVLIKAGRITVNGKVGRLNDDVSESD
                     EVRIGQQIIKLRQSRYIILNKPAGYITTLKDPQNRPKVTDLVKVAERVVPVGRLDYDT
                     TGALLLTNDGQLAHRLMHPSYKVDKTYEATVRRNVTPKILNMLSIGIKLEDGQTAPAK
                     AKKLSESKVELTIHEGRNHQVKRMLEAVGLPMRRLHRSKYGPLTIEGLKPGQWRDLTA
                     KEVELLKRTDSV"
     gene            complement(3872..3948)
                     /locus_tag="VEH48_02290"
     tRNA            complement(3872..3948)
                     /locus_tag="VEH48_02290"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3912..3914),aa:Ala,seq:ggc)
     gene            complement(4076..4152)
                     /locus_tag="VEH48_02295"
     tRNA            complement(4076..4152)
                     /locus_tag="VEH48_02295"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4116..4118),aa:Ala,seq:cgc)
     gene            complement(4361..>4758)
                     /locus_tag="VEH48_02300"
     CDS             complement(4361..>4758)
                     /locus_tag="VEH48_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HXY18227.1"
                     /translation="LPRATLDELMEVEGVGEIVAESILAWFADPDNHNLLKKFEKLGV
                     RPAHHSHSGGPLAGKNFVITGTLETMSREEAADKIRELGGTFQTSVGNDTDYLVVGAN
                     VGENKLTKARELGTQQLTEKELLKLLDIK"
BASE COUNT         1013 a         1208 c         1257 g         1280 t
ORIGIN      
        1 ttaattttta accaggccgg gctctgacgt accagtttag tctggtcttt cgggttggcc
       61 aacagcactt taagggcttc ctcggcaaac acgccgtttt gcgaaccctt cgccaggact
      121 atcgctcctg gtttgatgac cggttttaaa taatcaccga ctgtgtaggg gttatcaaag
      181 gtgatgactt tgcagcctct ggtttccgcg gctggcgcta agtatttatt agcgtccggg
      241 ccaagagtca gcaccatgct tagatgcttg gggtcgcaaa attcgccgat tttacggtgt
      301 tcttcttccg atgatgggcc taattcattc atgttgccaa gtatcgcgat tttttgcggc
      361 gattccattt ggtaaaggct gctaagcgcc agcttgaccg ctgccggact ggcattatag
      421 gtatcatcaa gtatgaccga actattgatt ccggccaatc tttgtagccg tccgggcgcc
      481 gggcttatgg catgcaggcc atttttgatt tcttcgggct ccaaaccgaa cttgaccgct
      541 acggccgccg ccgccagcat actataaagc tccggcaaag ttttgttgga ggaatcaaac
      601 cgggccaatt gggtttgacc gctgctgagt actgctatgt ttttgtcgtt gacaagcctg
      661 tagtcggcat ttgtgcgtat ggcgtaggtc aaaatatccg gcaggctgtc caaatatttt
      721 ttatcgcaaa ggtcggcatt ggccagcacc agcccggaca atttactgac cgagagctct
      781 tctgccgcga cttcgtcaag cgtcccgaaa aattccatgt gctcgggtac aatagatgtc
      841 actacggcta cttccagctt caggtatttc tgaaactctg ctatctggcc cgggccgtcg
      901 gtaccaagct ccagaacgac tatttcataa gggtagggcc gacgcagatg cttctcgttc
      961 cgccaaaata cacctagcca ggcaaatggg ttgaaaaggc tcggcgtttt ttcgccaaaa
     1021 aaaacaaggg gcgcggtaac gatgtggttg tagttgccct cctgatgctg ggctttaaat
     1081 tttttatcca acacagtagc gattgccaat ttggtgctgg ttttacctat actgccaacc
     1141 actccaaccg ctttaaagtc attcttctta tacagccttc ttatctggaa gcctagcact
     1201 gcggcaaccg tctttttggc tatttctttc atcgattaaa gtatagcaag ctgggttagt
     1261 tggtgagggt cgtgaggtcg ctgccggttt tgacataagg agtgccatcg tccagtgtcg
     1321 ccgcaagcgt gtacccgctg caatcgatgg tcgtattatt acagcctgca ggtgtcggtt
     1381 gataggcgta gacgtgagcc gccggggaag ccgcgagggt ttgcgagttg ccggccgggt
     1441 cttgcaaggc gctgggatcc aagccctgca ggttttgcga tcgccaggag gcattattca
     1501 ggtcagctaa agtagggtag tacccggtgt ttgtgttata agcttcaacc tggccttgca
     1561 gggcattaat gtctgtcata cgtttagtgt cgttgacaga gctgccaccg cccagaagat
     1621 tcgagaaacc ggcgtcattc aacaactgga taatgttttc agctccggcg ggcgcctgta
     1681 catttaccgc gctgttgttg ggtgcgactg tcaggttaag cgacccggtg aaattattgc
     1741 cgtcctggac ggagccgttg accgtaaccg aattggtctg agtgttgacg gtgatgccta
     1801 ggctgccgct ggaaacgtcg ctgccggatt tgtcatggaa accgatgctg aacggatagc
     1861 tactgccccc ttggtaatcc tggccgatct caaagtaatt ggtgctgttg tttttatcgg
     1921 taaagcggat cttgtggaca agtttggtat ggaggtccac ccaaacatcc gcagtcctgg
     1981 ttgtattgag gctgttgacc acactggtaa cattgtcaca gccaatggcg ctggcgacgt
     2041 tttgggaatc gttagcgaag aatttcttga ggctgctgtt ttcaagattg gaacacaaag
     2101 atgttacaaa gctcttaaag ttctgtttgt taacggagac tttgaagtga taaacgctcc
     2161 ggccgtcctg tttttccttg ccgacgtttt gcgaaaccgt gaaggccatc ttggtcgggt
     2221 ctttggtaaa gacattttgg tcaccggcgt taccgacggc attaaggatg gaactgacat
     2281 ccgaagaagt gatttgcaga ccacccgtgg cactgcccgt atattggtca aagagcgtgt
     2341 ggtcaatgaa ataccatttg ccattgatgc cgttcagcac cgacgcgtaa ggcgagtccg
     2401 acccgcccaa aagcgtgccc aggccttgca agccgtcaat cttgaaataa acatccggtg
     2461 tgccgacgct tgatctaacc agtcgggtat caaaactgac cttgacgccg actgccgaaa
     2521 cgctgccggt aaactcgcca ttggtgccgt cgttgctgcc ctgtatgctg ccgtcagaag
     2581 cgaaagagcc ggtggtccgg aaagagccgc tgagcttgat acccttgttt ttggaaccga
     2641 cttggctgac ggcgtatttg gataaggcgt cgtaaccctt gccggtattg gccaaagccg
     2701 atgcccagat attggccggc ttatttggca tatagtagcc gaagtaggcg gccgcgccgc
     2761 ctccgccaat caataagact gccagaaccg gaataactac cgctttacgt cgcaggcggc
     2821 caaaaaatcc cttaggttca tccggagccg ccggctggtc gctctggaac agtatcgttg
     2881 gattaggtga cgggggattt ggatacgctg tcatcgaacc gggcatgaca gaaggcgcgg
     2941 gcggcgcggg ttgttcactc gccgctggtt cgggagtcgg ttgtgtgggg ttgaatgatt
     3001 ggccgggaag cggttccata actgtgattt tgcttaagct taactataca gagtctgttc
     3061 gcttaagcaa ctctacttct ttggctgtca ggtcccgcca ttggccgggt tttaagcctt
     3121 ctatggttag cgggccgtat tttgagcggt gcaaccggcg catcggcagg ccgacggctt
     3181 ctaacatccg cttgacttga tggttccggc cttcgtggat ggttaactct actttgctct
     3241 ctgataattt tttggctttg gccggcgcgg tttggccatc ctcaagtttg atgccaatac
     3301 ttaacatgtt aagtattttt ggcgtgacgt tcctccgcac tgtcgcttca taggtcttgt
     3361 ccaccttata gctgggatgc atcaggcggt gcgccagttg gccgtcattg gttagcaaca
     3421 gcgcgccggt ggtgtcgtaa tccaaccgcc cgaccggcac cacccgctcc gcgaccttta
     3481 ccagatccgt aactttgggg cggttttgcg ggtccttaag agtagttata taaccggccg
     3541 gtttatttaa gatgatgtag cgactttgtc gcaattttat aatctgttgg ccaattctta
     3601 cttcatctga ttctgatacg tcgtcgttaa gccgccccac cttgccattg actgttattc
     3661 tgcccgcttt aatcaagacg tccgcgccgc gccgcgaagc cactccggat ttagccaaaa
     3721 aaacattaag cctcatctac ttattgtaca taatcagggt ccccgccgtg cggagctgca
     3781 cggtggggga aagcagcaac aatgcaagac cccgcccaat tcgtgccact tgattgcgcg
     3841 aattgaggcg gggttgatat tagttgatgc gtggtggagc ataagggatt cgaacccttg
     3901 acctcttcca tgccatggaa gcgcgctagc caactgcgcc aatgcccctc gtcgcaactt
     3961 acgcacctgc ctcgttttgc cagtctagca aaagcttcgg cttagcgcta tgttgctcct
     4021 ctcaaatttc caaacggtga taagaccgcc gctttgaaaa tttgataaag aacagtggtg
     4081 gagatgcggg gagtcgaacc ccggacctct gcaatgcgaa tgcagcgctc tagccaactg
     4141 agctacatcc ccgcgtctcc gaacggctaa attatagcat tttaaggtgt taaaaagcaa
     4201 aaacccgccg tttggcggat tgttgcttac ttcgcgcagc ccacccgggg cccgggcgat
     4261 tttattttaa taatttttat ctttctcttc gcatccggct ctagcgagca atagccggaa
     4321 gggcccggtg cgcgaagctg ccctaattac acgcttacgc ttactttatg tcaagcaact
     4381 tgagtaattc cttctctgtg agctgctggg taccgagttc tctggctttg gttagcttat
     4441 tctcgccaac gttggcacct actactaaat agtctgtatc gttgccgact gaagtctgaa
     4501 atgtcccacc aagttcacgg attttatcgg cagcctcttc gcggctcata gtctctagtg
     4561 ttccagtaat cacaaagttt ttaccggcca gcgggccgcc gctgtggcta tgatgagccg
     4621 gcctaacgcc aagcttttca aatttcttca gtaaattatg gttgtcgggg tcggcgaacc
     4681 aagccagtat ggattcggcg actatttctc ctacgccttc tacctccatc aattcatcaa
     4741 gggtagcgcg tgggagtt
//
LOCUS       DASPTF010000071        23486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_696725, whole genome shotgun sequence.
ACCESSION   DASPTF010000071 DASPTF010000000
VERSION     DASPTF010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 23486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23486)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23486
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696725"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VEH48_02305"
     CDS             complement(<1..213)
                     /locus_tag="VEH48_02305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HXY18228.1"
                     /translation="MLVPAFNEEKVVERSLQSIWNNTYENIEIIVISDGSTDGTAAAV
                     QRFINSRTRVYRQTVPEIVRTKNGLRR"
     gene            complement(380..1003)
                     /locus_tag="VEH48_02310"
     CDS             complement(380..1003)
                     /locus_tag="VEH48_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HXY18229.1"
                     /translation="MYPRINLMLKKSLSGLKSLKASLLIGLAALLVGLAVLANSLYST
                     WRAQQSAVKPVKQAFANTQVISGQPTLSGTPTHISIPSVGIDLQVIPGYFNAKNNSWT
                     LSLSDAQYATVTAPANNKKGLTFIYGHYRLHVFYTLPKVQPGALAIVTTDNGHTFTYT
                     FRDSVITSPNDTSLFSYQGKPILVLQTCTGLWYQNRQLFTFDLTKAA"
     gene            complement(1090..1251)
                     /locus_tag="VEH48_02315"
     CDS             complement(1090..1251)
                     /locus_tag="VEH48_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18230.1"
                     /translation="MTTVAGATTIAALPNTGGSRSVISYVALASVVFGTAVLISTAVR
                     FVAKRHYGA"
     gene            complement(1479..3239)
                     /locus_tag="VEH48_02320"
     CDS             complement(1479..3239)
                     /locus_tag="VEH48_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HXY18231.1"
                     /translation="MQYWPKIKKFFPQIHSLVRRHPLLAGLVLAVLLVAVLFWVARQA
                     AAPSRTEPAGPAVNLVTGQDWSHFTGANQDDKGVHITGLGRAIVNQDGTPNQTNPPVN
                     VRGPHLILKGDFSVQASIQQIPKASAASLYLYGAVPIIYDEWRYQTPQLCLKLSQKNL
                     ALSLWNGSGDQPVDQKSWNNPIGPTANIQVNVIKGNLEIWLNSRYIDSIPGHGIFSGG
                     QLWFGADESLDSPAWLLRSLSAAPLAGGQVQVIASPQLKIATSDPASLRSLARSNPRQ
                     IPIGAAIANYALFSDPAYRQLVATQFSMLTPENELKAQFTEPQPNVYTFTDADNLVDF
                     ALANGMTVHGHNLVFSEANPQWMQSAPLDQRQQIMVDHITTEVKHFGSKINEWDVVDE
                     PLNDNDDDNGNSSDLRHNIWFAAMGENYINIAFQTAHAANPGAKLYINEYGLEADGLR
                     WDEFLALIKRLQSQGTPIDGVGFQAHVYEPGDEVDQATLTEHMKILADMGLLVRVSEM
                     DVYGDSPSHQAQQYAGVLQACLDSPNCTSFNTWGVTDRYGSTTQYHAYPLELGNDLIW
                     DAGLKPKPAYRDLQNILNER"
     gene            3371..5158
                     /locus_tag="VEH48_02325"
     CDS             3371..5158
                     /locus_tag="VEH48_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXY18232.1"
                     /translation="MQLIRKFKFVAAAIAFSASTFMIPLQARAASATTPINTGAKVSF
                     TFDDGLTSAYTNAAPTLAKYGYTGTDFVITGCVGMTTTPNTCHADTDRSYMTWAQIQA
                     LQNTYHWEIGSHTVDHACLASSAAQDADDCSAPYGGLTIAQVDAELANSKAALAAEGI
                     NATDMATPYGDYNQSVLAEIAKYYASQRGFADTGYNATPYNDYIIRDQIATGGVSLAT
                     LEGYVNQAITNKQWLVLTFHDIQANASSNPDDYQYSTANLGALAAYIKSKSVPVVNIN
                     NGLISGTNLFTDYTFDNGLANGWTTDNATYVKKDTGNHGSYPSPTNSISMTATTKDVH
                     LFSPKVAVDSSSTYVLQSFLNLTKINTGYNMGYYVDEYDANGNWISGQYKQGVSFAWP
                     QTTGFEYKPTSSNVKSAALQVIVPANSEIQAYVDNFQWINENNPTTPPPVQTNLVANG
                     TFDAGIASGWTTDGAPYIAADNGNNGSPANPVNSIKLTSTTKNIHLFSPKVAVSSTNS
                     YNLSAYLNIKQISGTASEIGFYMDEYDANGNWISGKYITGDHVLGANTISFNYTPSSA
                     NVKQASLQVIVVANSNIVAYLDNVVWVQN"
     gene            5214..5288
                     /locus_tag="VEH48_02330"
     tRNA            5214..5288
                     /locus_tag="VEH48_02330"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5247..5249,aa:Gly,seq:tcc)
     gene            5438..5965
                     /locus_tag="VEH48_02335"
     CDS             5438..5965
                     /locus_tag="VEH48_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18233.1"
                     /translation="MAEFIWFYGASGAGKASIINKIADGEFDLPGVRLAVVCRESLEF
                     GRRQRIDLLERELGKYAVSSDAVLIKGQGVDLGKLRLPYRIHEAYPDLRQQVVFVYAK
                     PDLLPQRIEFRRDNYWPSPKHDFIKETESQIANVAKLCDDLAIKPLLIDNSGKRPQIK
                     TSHDLAGFYQEAKAS"
     gene            complement(5962..6750)
                     /locus_tag="VEH48_02340"
     CDS             complement(5962..6750)
                     /locus_tag="VEH48_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HXY18234.1"
                     /translation="MRLSHKLSIVTAVASVSLAGLIPAAALAAGSAGLGLSPSGGSFA
                     VGSTHSLAIYENGTDVNVVTANLSYDASSLQFLGASCGGAFPGAVQESGGGGAVALSC
                     YVSPGSPAVSGSQEVGVVSFKALAGGGSTAVSFASSSIVASTDSTFNAWNGNTAGGSY
                     SFYTPAPIANSTAPATPAPQVQAATKAAKKTAPKVSKTATYITPATTKGHLSAWIYVP
                     LGLIAAVAGIAYVFRKELAKNQKRIKRTAKNLRKTTWLKAFQRS"
     gene            complement(6753..8363)
                     /locus_tag="VEH48_02345"
     CDS             complement(6753..8363)
                     /locus_tag="VEH48_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXY18235.1"
                     /translation="MKLNELMNKFKRGTSLAAAAAFATTILATNVATVAAAPVLPTPR
                     ISFTFDDGLTSADANAAPILAKYGFAGTDFVISGCVGMSTTPNTCHANNDASYMTWAQ
                     INDLKNTYHWEIGSHTVDHACLASNATADPDDCSAPYGGLTPAQVNSELTTSALTIGS
                     NVGVTPTDFATPYGDWTPPVLAQIAAVYASHRGFADSIDQNGDGVIDHGNTYPYNDYL
                     LYDYQVQGDTAGGGVSVAQVESMINQTITNNQWLILTMHDILPNASTNPADYQYSTTN
                     LDAICAYIKSLNIPVVTINQGLVTNTGNLLPNSSFDTALSSNTTDTTSWSTDDPTDIH
                     QDTGGNGNYPSPANSVVLNGTTKDIHLFSPQVPIVGTKTYVLKNYLNVTNMTVAAGHE
                     IDFYIDEYDASGNYLQTKFMKSEVGDSAELNGAWVEELAFTYTPTNATVAKARLQLVV
                     TANSGAKAYLDNSQWFAEDGSTTGGTGSGSAVAGDVNGDGMVNALDLSILLSHWNSSG
                     ATLATGDLNGDGTVNALDLSILLTNWGK"
     gene            8526..8602
                     /locus_tag="VEH48_02350"
     tRNA            8526..8602
                     /locus_tag="VEH48_02350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8560..8562,aa:Arg,seq:tct)
     gene            8722..9273
                     /locus_tag="VEH48_02355"
     CDS             8722..9273
                     /locus_tag="VEH48_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18236.1"
                     /translation="MQLSDKLHTFVPVFDWSWWQIFLLTFFIDWLAIHIILYLEPLGI
                     GKPFGKSKRQHFRSNTYGDIFLPIAVASSIVVARGLNVQDVWYTSRWWNIAVVVFGYM
                     LVIALDVQSDFTLRQSLTASKLWHTLIAFPIMFYLGVITLIPLISVHKPLWAFILAIF
                     GYLGWLFFVIWDFRKPPDFRFAA"
     gene            9439..9660
                     /locus_tag="VEH48_02360"
     CDS             9439..9660
                     /locus_tag="VEH48_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18237.1"
                     /translation="MRGEGLENAPSEADELAYIASEAGKLIRKLFRDQPVSRTPVEEV
                     VHFAPGSEQLVIPAYASPPPLNRQETVIH"
     gene            10265..10340
                     /locus_tag="VEH48_02365"
     tRNA            10265..10340
                     /locus_tag="VEH48_02365"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10298..10300,aa:His,seq:gtg)
     gene            10350..10886
                     /locus_tag="VEH48_02370"
     CDS             10350..10886
                     /locus_tag="VEH48_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HXY18238.1"
                     /translation="MTSNLNPFSLPIGRGGQRPRTDDEYNKFVLQTVQPGLAGLMDGS
                     VSTLAPIFAAAFATHKPHVVLLIGISAAVGAGISMAFAEALSDTGELTGRGRPLRRGI
                     IIGGMTFLGGIFHTLPFLISNIHTALTFAYLIVGIELTIIAYIRYRYFEMKFWLSVLQ
                     VIVGGGLVFLAGILIGSA"
     gene            complement(10889..11488)
                     /locus_tag="VEH48_02375"
     CDS             complement(10889..11488)
                     /locus_tag="VEH48_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18239.1"
                     /translation="MADILWRTKILRGENQRKFTHITVGAFAAFWPWLISWQSIQLIA
                     LAMTVVVLINRPFQILHIRGISKKDRETYGDVLLAPAILMCALITTNKIFFALAVLNM
                     ALADGIAAIVGINFGRHWRYKVFHQAKTVIGSMAFWFVSLCIFAVGLLFLHDSLNFIQ
                     YTVLLLALPPVLMVVENVAGLGLDNIFVPLAALIALQIL"
     gene            complement(11589..11664)
                     /locus_tag="VEH48_02380"
     tRNA            complement(11589..11664)
                     /locus_tag="VEH48_02380"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11629..11631),aa:Pro,seq:cgg)
     gene            11915..12721
                     /locus_tag="VEH48_02385"
     CDS             11915..12721
                     /locus_tag="VEH48_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18240.1"
                     /translation="MPFSAALKESGRGALKIIKDTLRVVRQHPEITFYPYAAVFFISI
                     TYPLVSATVFASWYRRIFSDTSGLVPGHARAIIGLVVFSAFYTALVTAYFTCATSVSV
                     IAKLDSLEVPRFYGLLQVARHFFRVTRFALISVFFFPIGIYAQRKKLPSGWIGVLGSS
                     LTLHMAQVAPAVLATNKPLGATVRNSVDSLGRFWREGLVLKITMYLAAFVVVVLPKLI
                     QHGFFHGYTASKIGWAVSIEIAASGLVGFKVLNSIFTAVMYHEARSETKV"
     gene            12734..12811
                     /locus_tag="VEH48_02390"
     tRNA            12734..12811
                     /locus_tag="VEH48_02390"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12769..12771,aa:Pro,seq:tgg)
     gene            complement(12842..14356)
                     /gene="rny"
                     /locus_tag="VEH48_02395"
     CDS             complement(12842..14356)
                     /gene="rny"
                     /locus_tag="VEH48_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HXY18241.1"
                     /translation="MIVVTIVLAIAGLLVGGGGATVVNRRLGKGQADKAKKELQKAKK
                     EAEETIKKARDEADKRVEEARKDEQKRRGEIKDLEQRLLERQEALDKKLDELDKRSEG
                     LRKAEGEVEALKNEIRDIRTKQQEKLEKIAKLTKTQASEKLMEMTERDIKDDLIGLVA
                     KLQNEAKDEAEEQAAVVLTAAMERMASDVTAERTVSAVKLEDEEMKGRIIGKEGRNIQ
                     ALQRATGVDILVDETPGMIILSSFDPVRREVARQAMEMLLKDGRVHPGRIEEVVEKAQ
                     KQVQKDVVRAGEDAAREVGVIGIPKEILQLLGELKYRTSYGQNVLKHSTEIAQLAGVL
                     ADELGADVKTAKYAGLVHDIGKALSHKVEGKHHHISGEMLRKYGAPEEVALAAEQHHD
                     DMEATTVEAMIIRVCDAISAARPGARNISAEGFAERMKELENTALGFDGIEKAYAISA
                     GREVRVFVKPKSVDDLQAIRLARDIATKIESTMQYPGTIKVNVIRETRAIEFAK"
     gene            complement(14537..14929)
                     /gene="tsaB"
                     /locus_tag="VEH48_02400"
     CDS             complement(14537..14929)
                     /gene="tsaB"
                     /locus_tag="VEH48_02400"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXY18242.1"
                     /translation="MIILTLRTDKPEAEVGVFDGQKQLAYEKWLAHLRLAETLNHQIE
                     KILNKSSISAKELQGIVCYKGPGSFTGLRIGLSVANVLAYSYNIPIVATGGKNWVSQG
                     IQQLQAGRNQRLALPEYGRPATITKPHK"
     gene            complement(14926..15423)
                     /gene="tsaE"
                     /locus_tag="VEH48_02405"
     CDS             complement(14926..15423)
                     /gene="tsaE"
                     /locus_tag="VEH48_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXY18243.1"
                     /translation="MSTDMIWQLRSTASADTERLGEVLGSLLKPPAVIELWSDLGGGK
                     TTFVRGLARGFGSHDRVSSPTFTLNKIYKAEDSEIHHFDFYRLSDPGVVSDQLEESLQ
                     NDRVITVVEWSDIVRGVLPAARLVIEFQPVQDNPDERNITIHYPQSLRAIITELESRL
                     AESEP"
     gene            complement(15347..16636)
                     /locus_tag="VEH48_02410"
     CDS             complement(15347..16636)
                     /locus_tag="VEH48_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HXY18244.1"
                     /translation="MLFGLLFLARPLADAAAPPAMTVPQTLAISEIKITGDEFVVIQN
                     NTTAAIDVTGYWLYEFNNVNPQAAGASSSTQQLPAAKLDSGQALLLSDIAQNTCGAEL
                     AGKLSLSLGDSGGSLEILGSANGLIATVPADFVSWSSGASGQIPNVPSSSKDPKGVYY
                     RTAGGWQLADLDPLVPCQLDISAGGVVNTAVAKAAGAVPSVVVTAGSNLPGDDSGLVA
                     PQLSEILPNPAAPQTDANDEFIELYNPNSKPFDLGNFTLQVGLATTHKYTFPDNTFVQ
                     PQQFAVFYSSDTGLSLSNSGGQVELLDPAGNVVEQSDVYSTAKDNYAWIYADGKWQWT
                     TKPTPGAKNIVIAPAAAKRSSGSSGKKAVLAATTGSNSGGPGGGTLATAPSSLHPAIL
                     AGIGSLALLYALYEYRHDLAAALYRFRRYRAARRGAG"
     gene            16741..17262
                     /locus_tag="VEH48_02415"
     CDS             16741..17262
                     /locus_tag="VEH48_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18245.1"
                     /translation="MLRLSGSLRNRPVLSMRSGGPVATAVEPVINPHNLKILGWWCKA
                     PGGKELVLLADNVREIMPNGLAVNDDDALSAPEDLVRYKDILEIRFQLIDKPVKTKRQ
                     KLGKVSDFSYNDGLFVQKLYVARSLVKVFAAEDVLLIDRTQILEVTDHYILVRDSEVK
                     ATEEELAGALATA"
     gene            complement(17338..17937)
                     /locus_tag="VEH48_02420"
     CDS             complement(17338..17937)
                     /locus_tag="VEH48_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HXY18246.1"
                     /translation="MKVTALKAQIKNPQRVSIFVDEKYSFSLSLDELAKYRIKQGDDL
                     SAADEKKFKKLSADGKLRARALEWLLNRPHSEREFRDYLYRKKAGPEQTKIFVKEFSR
                     KRYLDNAKFAAWFVELQVRRGKSDRAIRSELFAKGINRELADKVLHQAETDEGVRLKA
                     IIAKKAKLPRYQKDPVKLKQYLMQQGFSYQLIKDTLKNT"
     gene            complement(17940..18983)
                     /locus_tag="VEH48_02425"
     CDS             complement(17940..18983)
                     /locus_tag="VEH48_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXY18247.1"
                     /translation="MRLHRLPLKSTLIFVCSLAVLVLVYVVGSEVGEPFTTKAAGNNQ
                     LISTNQPYVSLDLRVAGTAIYQGGPIKELTKLSPAAGVSRRYFSFAVPADNLTEYGLM
                     TTPASPAPAGGYPVVILIHGYIQPGAYSTKKAYLPDMEFYSQHGFVVLKPDLRGQGLS
                     IHAGQPEGAYYSMAYNTDIMSLIASVKKTTGLNGGDINLWGHSMGAYIALRAAVLSPD
                     IRSVILLSTPLDYPQDMYGDYRPRSDYANPVALKIRKAVLKQYGTPLNNPTFWNYTSP
                     MNFLIETHAYIQIHVGAKDKIVPPKFSSDLNLALNQLQLPHSYYVYPSGNHGLLSVRQ
                     QIYARSLPVLQRP"
     gene            complement(18986..19441)
                     /locus_tag="VEH48_02430"
     CDS             complement(18986..19441)
                     /locus_tag="VEH48_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18248.1"
                     /translation="MTGLNHAATGVLVAVIVKQPAIALPAAFLSHFVSDAIPHFDHKD
                     LIPKPNLYHVVVAIDLLLTAAFLTAMVMILKVAGWLIVACGLLAIAPDLMWLPVIIKA
                     RNVSFKGEGIMAKLKRFHKFVQWSEGPWGAYVEIFWLVLMLALILRIGR"
     gene            complement(19461..20504)
                     /gene="recA"
                     /locus_tag="VEH48_02435"
     CDS             complement(19461..20504)
                     /gene="recA"
                     /locus_tag="VEH48_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXY18249.1"
                     /translation="MGVAMADKTKIKKDSAPTAAGSGKAQALQLAVDQIEKQFGKGSI
                     MRLGEAHKIDIETIPSGSISLDLALGGGIPKGRIIEIYGPESSGKTTVCLHAVAEVQK
                     AGGTAAYVDAEHALDPAYAKRLGVNIDNLLISQPDSGEQALEVVEALVRSNAVDIVVV
                     DSVAALVPQAEIEGDMGDAQMGLQARLMSQAMRKLTGVISRTKCTVVFVNQLRMKIGV
                     MFGNPETTTGGNALKFYASVRMDIRRLSQIKSGDAVIGNHVRVKVVKNKVAPPFREAE
                     FDIMYNQGISYEGDVIDLAAVHGVVDKNGAWYEYGGQKIAQGREAAKQYLKDNPKVLD
                     EIAKKTLTAAQAE"
     gene            complement(20818..22401)
                     /locus_tag="VEH48_02440"
     CDS             complement(20818..22401)
                     /locus_tag="VEH48_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXY18250.1"
                     /translation="MRFFKRFAQSQEAFILPTILSFIVVAIILAAALVEVIDTNLSIV
                     NNNVQSQQAFNIAEAGINYYLWHLSHNPTDFKDGQSTPTTPDPTLGYGPYVHNYIDSN
                     AVNEGTFTLWIKPQGGGSTVATVQSTGQVKNSKYVRTIQAQIGEPSFASYGVLGDSAL
                     WFGNDETADGPIGSNQGIRMDGPNTDTVSSANTTYVPSTALGGDGHTSEPGVWCSSSV
                     TSPVNCNTRSKTNWLFPVSAVDFNQVNTSLCSMKKIAFASYSSTASLANQANACTQTP
                     STRTAAYLPQRSSSFSLTKGYLINLNTNGTYDLYNVNGENDRAATYSSALTTQLVASG
                     IVVPSSGVIFAEDNVWIRSNPTFHGRVTIGSGRLASSNNTDIVISDDLLYSTKNGQDA
                     IGLVAEGDVLVAPYAPPASGSFNYEIDAAILSENGNVEYPVYYRTASSRCTHGWVNSN
                     QQLIFYGSVSTRQTWTWNWLVGSACGDAANDPVNGWVSGIEHTVTSYDYNMLYSPPPS
                     YPLTAGFNILSWREVLTKP"
     gene            complement(22398..23015)
                     /locus_tag="VEH48_02445"
     CDS             complement(22398..23015)
                     /locus_tag="VEH48_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18251.1"
                     /translation="MSRLKEMRRTSRRAGFTIVELTIAITIAFLVSLTAYSIFSTLLN
                     QYFGLQADGSEFTNLASQSQRIANVLRGLTDIVSESANDLTVYAYFSPNDTYVSEIHY
                     YLNANSTALYADVTPMTANPPIGTPITGQKVTYTIIDNYYKLPGLNLFNYLDSSFNVL
                     SLPISDENSIKGIQINLAVPADFPTQSGNQEISLQISLRNRKTNL"
     gene            complement(23002..>23486)
                     /locus_tag="VEH48_02450"
     CDS             complement(23002..>23486)
                     /locus_tag="VEH48_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18252.1"
                     /translation="QYYLIAAVPYGGSNSLAPITVPTYDPTNPPTTTYSYAGTSYLQE
                     VRLMLSTNANLPRIFSLSPYDVSLSGGTINNFQFTVSGQKLSCGTSGSGCSSSVRFVQ
                     GGNTYTATCTGASAGVQLNCSVNLTGIGTGNAQLVVGTSSGSVSLPFSPQMGGLSVTP
                     "
BASE COUNT         5283 a         6019 c         6356 g         5828 t
ORIGIN      
        1 ccgcctcagg ccgtttttgg tacgcacaat ttccggtacg gtttggcggt agacacgcgt
       61 gcggctattg ataaagcgct ggacggctgc ggcggtgcca tcggtggagc cgtcgctgat
      121 gacaatgatc tcaatattct cataggtgtt gttccagatg gattgtagac tgcgctccac
      181 gactttctcc tcgttgaaag ccggtaccaa cacgctgacc agcgggagat gcgagtcaag
      241 cgaccgttgt ctgggtttgc gatgcctaaa agctttgata tcgtagatat tgccgccaac
      301 cagataaagc gcgaagtgca ccatattaat catgccgatg atgacaaaca ggtagaataa
      361 gatacttaaa ataatcatct taagccgcct tggttaggtc aaaagtgaaa agctggcggt
      421 tctggtacca caggccggta caggtttgta gcaccaaaat cggcttgccc tggtaggaaa
      481 acaggctggt gtcattcggc gaggttatca cgctgtccct gaaagtatag gtaaaggtgt
      541 ggccgttatc ggtagtgacg atagcgagcg ctccgggctg gactttaggc agggtataga
      601 atacgtgcaa ccggtagtgg ccatagataa aagtcaggcc ctttttgttg ttagctggtg
      661 ccgttacagt ggcgtactgg gcgtcgctaa ggctaagtgt ccagctgttg tttttggcat
      721 tgaaataacc gggtattacc tgcagatcga tgcccacgct ggggatgctg atatgcgtcg
      781 gcgtgccgga taaagtcggc tggccgctga taacttgcgt gttggcaaaa gcctgtttaa
      841 ccggtttgac ggcgctttgc tgagcccgcc aggtggaata caggctgttg gccagtacgg
      901 ccaagccgac tagcagggct gccagtccga ttaataaact ggcttttagt gatttgagtc
      961 cggaaagtga tttcttaagc ataagattta tcctcgggta cataaaaagg ttggggcctt
     1021 tttttgccgg tctcttgttc aaagatctgc tcaccacctc tcctccaacc ttaatatgct
     1081 gcttgccgtt taggcgccgt aatggcgctt ggcgacaaag cgaaccgctg tgctgatcag
     1141 tacagccgtg ccaaacacaa cgctggctaa ggctacgtaa cttatgacgc tacggctgcc
     1201 gccggtattc ggcaaggcag caatggtagt agcgcccgca acagttgtca ctgcgccagc
     1261 tcctaaacca ccgacatagt ccatgctgca ctccttctta gttatttacc agcgaacctg
     1321 gataccagcg aaccagcccc ttggctccca ccccgggtaa tctgctatgt cccagatcca
     1381 actgggtcta atagtacact acttatgaca gtttgtaaag actattttat aaaaacatta
     1441 gcgtaataaa ttacccctgt aaattctcgc caaagcgctt atctttcgtt taatatattt
     1501 tgcaaatcgc ggtaagccgg cttgggtttg aggccggcgt cccaaatcag gtcattgccc
     1561 agctccagcg gataggcatg gtactgggtg gttgagccat aacggtcggt gaccccccac
     1621 gtattaaagg aagtgcagtt cggcgaatcc agacaggcct gcaatacccc ggcgtattgc
     1681 tgggcctggt gggacggact atcaccgtaa acgtccattt ccgagacgcg caccagcagg
     1741 cccatgtcag ctaggatttt catatgctcg gtcaaagtgg cttggtcgac ttcgtcaccc
     1801 ggctcgtaga catgggcctg gaagccgacg ccgtcaatcg gcgtgccttg gctctgcagc
     1861 cgtttgatga gcgctaaaaa ctcgtcccag cgcaggccgt cggcttccag gccatattca
     1921 ttaatataaa gcttggcacc agggttggcg gcgtgcgctg tctgaaaggc aatattgata
     1981 tagttttcgc ccatagccgc aaaccagata ttgtggcgca ggtcactgct gttgccattg
     2041 tcatcgtcat tatcattcag cggttcgtca accacgtccc actcgttgat tttgctgccg
     2101 aagtgtttta cctcggtggt gatatgatca accataatct gttgccgctg gtccaacggc
     2161 gcgctttgca tccactgcgg gttggcttca ctgaaaacca ggttgtggcc gtgtacggtc
     2221 atgccgttgg ccagtgcgaa atcaaccagg ttgtcggcgt cggtaaaggt gtaaacgttg
     2281 ggctggggtt cggtaaattg ggccttgagc tcgttctcgg gcgtaagcat actgaactga
     2341 gtagccacta gctgacgata agccgggtca ctgaacagag catagttggc tatggccgcg
     2401 ccgataggaa tttgtcgggg gttggaccgg gccagactgc gcagactggc aggatcactg
     2461 gtggcgattt taagctgggg actggctata acctgtacct ggccgccagc tagcggcgcc
     2521 gcactcaacg accgcagcag ccatgccggg ctgtccagag attcgtcggc accgaaccaa
     2581 agctggccgc cagagaatat gccgtggcca gggatgctat ctatatatcg gctgttgagc
     2641 cagatctcta agttaccttt aataacgttg acttggatat tggctgttgg tccaatcgga
     2701 ttattccaac ttttttggtc taccggctgg tcgccgctac cgttccaaag gcttagagcc
     2761 agattttttt ggctaagctt caggcacagc tgcggggttt gataacgcca ctcatcataa
     2821 atgatgggca cggcaccgta taagtagagg gaggccgcgc tggctttggg aatctgttgg
     2881 atgctggctt ggacgctaaa atcgcccttg agtataagat gtggaccgcg gacattgacc
     2941 ggcgggttgg tttggttcgg cgtgccatcc tgattaacaa tcgcccggcc cagaccagtt
     3001 atatgtacgc ccttgtcgtc ctggttcgcc ccggtaaaat gcgaccagtc ctggccggta
     3061 actagattga cggccgggcc ggctggttca gttcttgagg gagcagcagc ctggcgggcg
     3121 acccaaaaca acacagcgac cagcaagacg gctaaaacca agccggcgag cagcgggtgg
     3181 cgccgaacca gcgagtggat ttgcggaaaa aatttcttaa tctttggcca atattgcata
     3241 aaaagctaca atttactgtt taaaaatgct attgattttt taacaattat attatagtat
     3301 aagagctgaa ggtgggcaag cataaacaaa aaccctaaac taatttaagg agttagaaag
     3361 gttttaagat atgcaactaa ttcgcaaatt caagtttgtg gctgccgcca tagcgtttag
     3421 cgcctcaact tttatgattc cactgcaggc ccgagctgca tctgcgacca cacctatcaa
     3481 taccggcgcc aaggtttctt tcacctttga cgacggcctg accagcgctt ataccaacgc
     3541 cgcgccgaca ctcgctaaat acggctatac cggtaccgat tttgtaatta ccggctgtgt
     3601 ggggatgaca accacgccca acacctgcca cgccgatacc gaccgcagct acatgacctg
     3661 ggcccagatc caggctcttc agaacactta ccactgggag atcggctcgc ataccgtcga
     3721 tcacgcctgc ctggccagca gtgccgctca agacgcggat gactgttcag ctccatatgg
     3781 cggcttgacg attgctcaag tagacgccga actggctaac agcaaagccg ccttggccgc
     3841 cgaaggcatc aatgctaccg atatggctac accttacggc gattacaacc aatcggttct
     3901 ggccgaaatc gccaagtatt acgcttctca gcgcggcttc gccgacaccg gctacaacgc
     3961 taccccttac aacgattaca tcatccgtga ccagatagcc accggcggtg tttcgctggc
     4021 caccttggag ggttatgtca accaggccat caccaacaaa cagtggctgg tattgacctt
     4081 ccacgatatc caggccaatg ccagttctaa ccccgacgac taccagtaca gcaccgccaa
     4141 tctgggcgcc ctggccgctt acatcaaaag caaaagcgtg ccggtggtga atatcaacaa
     4201 cggtctgatt agcggcacaa acctctttac tgattacacc tttgataacg gcttggccaa
     4261 tggctggacc actgataacg ccacctatgt gaagaaagat accggcaacc acggcagcta
     4321 ccccagcccg accaattcaa tctctatgac cgctacaacc aaagacgtgc acctgttctc
     4381 gccaaaagtc gccgttgatt ctagcagcac ctatgtgctg cagagtttct tgaacctgac
     4441 caagattaac accggttaca acatgggcta ttacgtagac gaatacgatg ccaacggcaa
     4501 ctggatttcc ggtcagtaca aacagggcgt cagtttcgcc tggccgcaga ctaccggttt
     4561 tgaatacaag cctacctcgt ccaacgtcaa gagtgccgct ctgcaggtga ttgtgccggc
     4621 gaactccgag attcaggctt acgtcgataa tttccagtgg atcaatgaga ataatcccac
     4681 cactcctccg ccggttcaaa ctaacctggt agccaatggc acgtttgacg ccggcatcgc
     4741 cagcggttgg acaactgacg gcgctccgta catcgctgca gacaacggca ataacggcag
     4801 cccggccaac ccagttaact ctatcaagct gacttccacg actaaaaaca tccatctgtt
     4861 ctctcccaag gtagctgtta gttccactaa ctcttacaac ttgagtgcat acttgaatat
     4921 caagcaaata agcggtacgg cctcggaaat cggcttctat atggatgagt acgacgccaa
     4981 cggcaactgg atttccggta aatacatcac tggcgaccat gtgctgggag ccaataccat
     5041 cagctttaac tacacaccca gctcagccaa cgtcaaacag gccagcctgc aggtcattgt
     5101 ggttgccaac tccaatatag tggcttacct ggacaacgtc gtctgggtcc agaactaaag
     5161 tcagccgaca cctgtagttt gctataatct tatctgttac atccagtaac agtgcgggtg
     5221 tggtataacg gctattactc cagttttcca aactggttac gggggttcga ctccccccac
     5281 ccgcaccacg catcgactaa ttacagccaa actagaaaaa cgcgctggtc aagtagcgcg
     5341 tttttggttt ggcctggtat tgccgccgct tttccccgcc gcgcggctcc gcacaccagg
     5401 gattccaatt caatccgaat atgtaacaat tgtatatgtg gctgaattta tttggtttta
     5461 tggggcgagc ggcgcgggca aagcttctat tatcaataaa attgcggatg gagaatttga
     5521 tttacctgga gtcagactgg ccgtcgtctg ccgtgaaagc ctggaatttg gtcgccggca
     5581 gaggattgac ttactagaga gagaacttgg taaatatgcc gtctcgagcg atgccgtgtt
     5641 aatcaaaggc cagggtgttg atctcggcaa attaagactg ccctaccgta tccacgaagc
     5701 ctatccggac ttgaggcagc aagtagtttt tgtatatgcc aagccagatt tgttgccgca
     5761 gcgcattgag ttccgccggg ataactattg gccgagccca aagcatgact ttataaagga
     5821 aaccgaatct caaatagcca atgttgctaa attatgtgac gatcttgcta ttaaaccctt
     5881 actaattgat aactcaggca aacggccgca aataaaaacc agccatgatc tggctggttt
     5941 ttatcaagaa gctaaagcct cttaggagcg ttggaaggct ttaagccagg tggttttgcg
     6001 caggttctta gcggttcgct taatgcgttt ctggtttttg gctagttctt tgcgaaagac
     6061 gtaggcgatg ccggctaccg cggcaatcaa gccgagcggc acgtaaatcc aggccgaaag
     6121 atgaccctta gtagtggctg gcgtgatgta ggtagcggtc ttagatactt taggggcggt
     6181 ttttttggcg gccttggtgg cggcttggac ctggggagca ggtgttgccg gcgctgtact
     6241 gttagcaatc ggtgcaggag tgtagaaact gtagcttcca cctgccgtat tgccgttcca
     6301 ggcattaaaa gtactgtcgg tgctggcaac tatagagctg ctagcgaaac taactgcggt
     6361 cgatccaccg ccggcaagcg ctttaaagct tacaaccccg acttcttggc tgccgctgac
     6421 cgccggtgat cccggagaca cgtagcagct caaggcgaca gcgccgccgc cgccggactc
     6481 ttgtacagct ccgggaaacg cgccgccaca ggaagcacca agaaactgca agcttgacgc
     6541 atcatagctc aagttcgcag taaccacgtt tacatcagtg ccgttttcgt aaatcgccaa
     6601 gctgtgtgtg ctgccaacgg caaagctgcc gccgctgggc gataaaccta agccggctga
     6661 tcccgcggcc agtgccgcag ccggaattaa acccgccaag cttactgacg cgacggccgt
     6721 aacaatactt aatttatgac tcaatctcat agttatttac cccaattagt tagcaatatt
     6781 gatagatcca gcgcgttcac cgtaccgtca ccgttaagat cgccggtggc aagcgtagcg
     6841 ccgctactat tccagtgact tagcaatata cttaagtcca gagcattgac cataccgtcg
     6901 ccgttaacat caccggctac ggcagacccg gagcccgtgc cgccggtagt agagccatct
     6961 tcggcaaacc actgggaatt atccagatat gccttagcgc cggaattagc ggtcaccacc
     7021 agttgcaggc gggctttggc aaccgtggcg ttggtcggtg tgtaagtaaa ggccagttcc
     7081 tccacccagg cgccgtttag ctcagcgctg tcgccaacct ccgacttcat gaacttggtc
     7141 tgcagatagt tgccgctagc gtcgtattca tcgatgtaga agtctatctc gtggccggcg
     7201 gcgactgtca tgttagtgac gttaaggtag ttcttaagca cgtaggtctt ggtgcctact
     7261 atcggcactt gcggcgaaaa cagatggata tctttggtgg taccgttaag tacaacggag
     7321 ttggccgggc tggggtaatt gccgttgccg ccggtatcct ggtggatgtc tgtcgggtcg
     7381 tcggtgctcc aagaggtggt atcggtagtg ttgctactga gtgcggtgtc aaaactggag
     7441 ttcggcagca ggttgccggt gttagttaca agaccctggt tgattgtcac aactgggata
     7501 ttaagacttt taatataagc gcaaatcgcg tccaggttgg tagtgctgta ttggtagtca
     7561 gccgggttgg tgctggcgtt gggcaagatg tcgtgcattg tcaatatcaa ccactgattg
     7621 ttcgttatcg tctggttgat catgctttca acctgtgcga ccgaaacgcc gcccccggca
     7681 gtgtcgcctt ggacttggta gtcgtatagc aagtaatcgt tatatggata ggtgttgccg
     7741 tggtcaatta cgccgtcacc gttttggtcg atactgtcgg caaagccgcg gtgcgaggcg
     7801 taaaccgcgg cgatttgggc cagcaccggc ggcgtccagt cgccataagg cgtcgcaaaa
     7861 tcggtcggcg tgacgccgac attggagccg atggtcaagg cgctggtggt cagttcactg
     7921 ttcacctggg cgggcgtcag gccgccatag ggagctgaac agtcatccgg gtctgcagtg
     7981 gcattgctgg ctaagcaagc gtgatctacg gtatgcgagc cgatttccca gtggtaagtg
     8041 ttttttaggt cgttaatctg cgcccaggtc atgtagctgg catcattgtt ggcgtggcag
     8101 gtattagggg tggtgctcat acctacacag ccgctaatca caaaatcggt gccggcaaag
     8161 ccgtacttgg ccagaatcgg cgcggcgttt gcgtcggcgc tggttaaacc gtcgtcaaat
     8221 gtgaatgata tacgcggcgt tggtaaaacc ggggcggcgg ccacagtcgc cacattagtt
     8281 gccaaaatgg tggtggcaaa agccgcggcg gcggctaggc tggtacctcg cttgaactta
     8341 ttcattaatt catttagttt catactgttt ttgctggctc cgataagtta gtaaaaaata
     8401 tgcgcaggga cttttctatc gccccctttg gagctggatt atagtaaaga tttgttattc
     8461 atgcaagcat atgcattaat aattatttta aaaggctgcc gccatgctat aattacccct
     8521 gttcagcccc ggtagctcaa cggattagag cgagtggctt ctatccacta ggttgcaggt
     8581 tcgaatcctg cccggggtac catttgaaag ttgaaagagg tcgaaagcag cactatttcg
     8641 aactgttatt ttgacttcgg aggtgctaga agttgttcga atctctttgg ggtcatagta
     8701 gctaggttta tactttagaa aatgcaactc tcagataaat tgcacacttt tgttccagtc
     8761 ttcgattgga gctggtggca gattttttta ctaaccttct ttattgattg gttggcaatc
     8821 cacataatcc tatatcttga acccttaggt ataggcaaac cattcggtaa aagtaaaagg
     8881 cagcatttcc gctcaaatac ttacggtgac atattcctac ccatcgcagt tgcttcgagc
     8941 atcgttgtag ctagaggctt gaatgtacaa gatgtctggt atacatcaag gtggtggaat
     9001 atcgcagttg ttgtttttgg atatatgctt gtgattgccc ttgatgtgca aagtgatttc
     9061 acgctacggc agtctttaac tgctagtaag ctttggcata ccttaattgc ctttccgata
     9121 atgttttatc tgggagtaat aactctaatc cctttaatta gtgttcacaa gccattatgg
     9181 gcatttatct tagctatatt cggttatctt ggctggttgt tttttgtcat ttgggatttt
     9241 agaaagccgc cagattttag gtttgcggcg tagttaatca atagtcaaaa cacagttttg
     9301 ctcgttccac ctctttagct atactgacaa taatcccgta atacttcgtt cggagtatgt
     9361 ctagaacggg gtaccaactt ttcagatgta tttttgagtt ctggtttttg tcttcattgg
     9421 tttatactaa cagtaagtat gcggggtgag gggttagaga atgctcctag tgaggctgat
     9481 gagctggctt atatcgccag tgaagcgggc aagttgatac gcaagctatt tagggatcag
     9541 cccgtcagca ggacgccggt ggaagaagtt gttcatttcg ctccgggcag cgagcagctg
     9601 gtaataccgg cttatgcttc cccgccacct cttaatcgtc aagaaacggt aatccactag
     9661 cctctccccc tgtttagtgc tataatcacc tctgttacgg cgaatgtagc tcagctggtt
     9721 agagcggcgg gttgtggagc tgttcttgat ttactatctg gtctacctct gttaaaatat
     9781 aaaggtgcca gtattcaatt gtcgggtttg tggacgggaa aaatatatta agcctagcca
     9841 cgcaatgagc ggttacggca aatattgttc acagaaatgt aacgccgaat cccagaaaaa
     9901 cggacagctg tttgcatgtg acatttgcgg taaacaaacc tacagaagct tgaaggacca
     9961 gaaacgatca aaaagtggca agtttttctg tagcaaatcc tgtcaaacta tttggcgtaa
    10021 cctgacatac gtcggtgagg gacacgcgaa ttggaaaggt ggtacatatt cgtatagaga
    10081 tgccctgcgc cgtgccaaaa ttccagctac ctgcgcaagg tgcaagagta aagataccag
    10141 aaccctaact gcccaccaca aagatagaaa ccgacagaat aattcaattt ctaacttgat
    10201 ttggctgtgc cataactgtc attatctagt acatcactac aagagtgagt caaagaattt
    10261 tatggtgcct gtagcttagt ggttaaagcg ccagtttgtg gaactggaaa tcgagagttc
    10321 aaatctcttc aggcacccca cgataatcaa tgactagcaa tcttaaccct ttcagcctgc
    10381 cgatcgggcg gggcggccag cgcccgcgga ccgatgacga atacaataag ttcgtcctgc
    10441 agacggtcca gcctggtctg gccgggttga tggacggctc ggtatccacg ctggcgccga
    10501 tttttgccgc cgcttttgcc acgcacaaac cgcacgtggt gttgttgatt ggtatttcgg
    10561 cagctgtggg cgccggcatc agcatggctt ttgctgaggc cctgagtgac accggcgagt
    10621 taaccgggcg cggccggccg ctgcgccgcg gcatcattat cggcggcatg accttcctgg
    10681 gcggcatttt tcacactctg cccttcctga tttccaacat tcacaccgct ttaacctttg
    10741 cctatctgat cgttggtatc gagctgacaa taatcgcgta catccgctac cgttactttg
    10801 agatgaagtt ctggctgtcg gtgctgcagg tgattgtcgg cggcggtttg gtttttctag
    10861 cgggaattct gatcggcagt gcctaaactc acagtatctg cagggctatc agggcggcca
    10921 gcgggacaaa gatgttgtcc aagcccagcc ccgcgacgtt ctccaccacc atcagaaccg
    10981 gcggcagagc cagcagcagc acggtgtatt ggatgaagtt caagctgtcg tgtaaaaaca
    11041 gcaggccgac ggcgaaaatg cacagcgaca cgaaccaaaa ggccattgag ccaatgaccg
    11101 ttttagcctg gtgaaaaacc ttgtatcgcc aatgacggcc gaagttaata ccgacaatcg
    11161 ccgctatacc gtccgccagc gccatgttca gaacagccag ggcgaaaaat atcttattgg
    11221 tggttattaa cgcgcacatt aaaatcgccg gagccaacag tacatcgcca taggtctcgc
    11281 ggtctttctt gctgataccg cggatatgca ggatctgaaa gggccggtta ataagcacga
    11341 cgaccgtcat agccagggcg atcagctgga ttgactgcca actgatcagc cacggccaaa
    11401 aggccgcgaa agcgccgacc gttatgtgcg tgaatttgcg ctggttttct cccctgagga
    11461 ttttagtcct ccataaaata tcagccatga ttaccaagat aaaaacgcct agcagacata
    11521 caataagcgc aagcattatc caaattgtac cccatttttt gattctggat aaaggatgtg
    11581 ctcaattctg gtcggagtgg gcggacttga accgccggcc tcagcgtccc gaacgctgcg
    11641 cgctaccaac tgcgccacac tccgtcacaa cagaggtaat tatagcagat tgtgttaagc
    11701 gagcccctgg ttttgccgaa catccaggag ccctgattag atgttgtgca aaataggcgc
    11761 ccttgggcgc gaaagtataa atcaactatt tggagattca ttcttgagcg agcctgtttt
    11821 gcactaacgg ctactcacgg cgacgcagcg ttcggcaaaa gataggggca agcgttattt
    11881 gccgattacg ctttaagtct gatataaaac tagcatgccg tttagcgctg cgctgaaaga
    11941 atcgggccgg ggcgctctaa aaatcatcaa agacactctg cgtgtcgtta ggcagcaccc
    12001 ggaaatcact ttttacccct acgcggcggt atttttcatt tccatcacct acccactggt
    12061 cagcgcgacg gtttttgcca gctggtaccg gcggattttt tcggatacct ccggcctggt
    12121 gcccggtcac gccagggcga ttatcggact ggtggttttc tcggcttttt atacggcctt
    12181 ggttacggct tactttactt gcgcaacttc ggtaagtgtg attgctaaat tagacagtct
    12241 tgaggtcccg cggttttacg gtttactaca ggtagcaagg cattttttca gagtcacccg
    12301 ctttgcgcta atttcggttt tcttttttcc tattggcatt tacgcccagc gcaaaaaact
    12361 gcctagcggc tggataggcg tcttgggcag ttcgttgacg ctgcacatgg cccaagtggc
    12421 gccggcggtt ttagctacca acaaaccgct gggggcaacg gtgcgcaact cggttgatag
    12481 cctcggccgc ttttggcgcg aggggctggt gctaaaaata actatgtact tagccgcctt
    12541 cgtggttgtt gtcctgccaa aattaatcca gcacggcttt tttcatggct acacagccag
    12601 caaaatcggt tgggccgtca gtattgagat cgctgcctcc ggcctggtcg gcttcaaggt
    12661 tctaaacagt atcttcacgg ccgtgatgta ccatgaagcg agaagtgaaa ctaaggtata
    12721 attaacctgt cagcggggag tagcgtagcc tggctagcgc accgcgtttg ggacgcgggg
    12781 gtcgcaggtt caaatcctgt ctccccgacc aagtaaaaat acttgactag tcaagtattt
    12841 tttatttggc aaactcgatg gcgcgggttt cgcggatgac gttaactttg atggtacccg
    12901 gatattgcat ggtcgattca attttagtgg cgatatcgcg cgccaggcgg atggcttgga
    12961 ggtcgtcaac cgatttgggt ttgacaaaga ctctcacctc gcggccggcg ctgatggcgt
    13021 aggccttctc gatgccgtca aaaccgagcg cggtgttttc taactctttc atgcgctcgg
    13081 caaaaccctc ggcgctgatg ttgcgggcgc ccggccgggc ggcgctgatg gcgtcgcaga
    13141 cccggataat cattgcttct acggttgtgg cttccatgtc gtcgtggtgc tgttcggcgg
    13201 ccagcgcgac ctcttccgga gcgccgtatt tgcgcagcat ctcgccggaa atatggtggt
    13261 gcttaccttc gaccttatgg ctgagggctt tgccgatgtc gtggaccagc ccggcgtatt
    13321 tggcggtctt gacgtcggcg ccgagttcat cggccagcac gccagccagc tgggcgattt
    13381 ccgtggagtg cttaagcacg ttctggccgt agctagtacg gtattttagc tcgcccagaa
    13441 gctgcagaat ctcttttgga ataccaatga caccaacctc gcgggcggcg tcttcaccgg
    13501 cacgcacaac gtccttttgc acctgttttt gagccttttc aacgacttct tcaatccggc
    13561 cggggtgaac gcggccgtct ttgagaagca tttccatggc ctggcgggcc acctcgcggc
    13621 gcaccgggtc aaagctggag agtatgatca tgcccggtgt ttcgtccacc agaatatcca
    13681 cgccggtggc ccgctgcagc gcttggatgt tgcggccttc cttaccgatg atgcggcctt
    13741 tcatctcttc gtcttccagc ttgacagccg aaacggtgcg ctctgccgta acatcgctgg
    13801 ccatacgctc catggccgcg gttaacacca ccgccgcctg ctcctcagcc tcgtccttag
    13861 cctcgttctg cagtttggca accaagccga tcaaatcgtc tttgatgtcg cgctcggtca
    13921 tctccatcaa cttctcactg gcctgggttt tggtcagctt agcgattttt tccagttttt
    13981 cctgctgctt agtgcggatg tcgcggatct cgttcttaag cgcctcaacc tcgccttcgg
    14041 ccttgcgcag gccttcggag cgcttatcaa gctcatccag ctttttgtcc agggcttcct
    14101 ggcgctccag cagccgctgt tccaggtcct tgatttcgcc gcggcgtttc tgttcgtctt
    14161 tacgggcttc ttccacgcgc ttgtcggctt cgtcgcgggc ctttttaatg gtttcctcgg
    14221 cctctttctt ggctttttgc agttctttct tagctttatc ggcctggcct ttgcctaacc
    14281 gacggttaac tacagtggcg ccaccgccac caactagaag gccggctatt gccagcacaa
    14341 tagtaactac tatcatttct tacccctttc ttggaggtag ctggaccgtc ggggttaaag
    14401 gctcactatc acttcattag ctttgaactt aaccggcgat cagaatgcta tcaaatattt
    14461 tgtgtcccaa aacggatctc actaaaagga aaatcctgaa tctaatatac gccacgcggc
    14521 ggcggcttgg caacggctat ttgtggggtt tggtgattgt cgccggccga ccgtattctg
    14581 gcaaagccag ccgttgattc cggccagctt gtagttgttg gatgccttgg ctgacccagt
    14641 ttttgccgcc agtcgcgacg attggaatgt tatacgaata ggctaggacg ttagcgacgc
    14701 ttaggccgat acgcaaaccg gtgaaactgc ccggcccttt gtagcagact atgccctgaa
    14761 gctctttagc cgaaatactt gacttgttaa gtattttttc tatctgatgg ttaagggttt
    14821 cggcgagtct caggtgggcc agccattttt cataagccag ctgtttctgg ccgtcgaaaa
    14881 cgcccacctc ggcttcgggt ttatcagtcc gtaaggttaa gataatcatg gttcggactc
    14941 tgccagccgg ctttccagct cggtaattat tgcccgcagt gattgcggat aatggatggt
    15001 tatattgcgt tcgtcggggt tgtcctgaac gggctggaat tcaatgacta gacgtgctgc
    15061 gggtagcacg ccgcggacga tgtcgctcca ctccacaact gtgatcacac ggtcgttttg
    15121 caaagattct tccagctggt cggacacaac acccggatca ctcagccggt aaaaatcaaa
    15181 gtggtggatc tctgaatctt cggctttata aatcttattc aacgtgaagg tcgggctgga
    15241 aacccggtca tggctaccaa agccgcgcgc caggccgcgt acaaaagtcg ttttgccgcc
    15301 gcccaaatca gaccagagtt ctataactgc cggcggtttc agtaagctac ccagcacctc
    15361 gccgagccgc tcggtatcgg cggaagcggt agagcgcagc tgccagatca tgtcggtact
    15421 catacagggc atatagtaag gccaggctgc ctatgccggc aagtatcgcc gggtgcaggc
    15481 tgcttggcgc ggtcgccaga gtgccgccgc ccgggccgcc gctgttgctc ccggtagtgg
    15541 cagccagaac cgcctttttg ccagagctgc cgctggacct tttggcggcg gctggcgcga
    15601 ttacgatatt cttggcgccg ggcgtcggct tggttgtcca ttgccatttg ccgtcggcgt
    15661 agatccaggc gtaattatct ttggccgtgc tatagacatc cgattgctcg acgacgttgc
    15721 cggccggatc caaaagctcg acctggccgc cgctgttgct caggctcaag ccggtatcgg
    15781 acgaataaaa caccgcgaat tgctgcggct ggacgaaagt gttatccgga aaagtgtatt
    15841 tgtgagtagt cgcaagacca acttgcagcg tgaaattccc caagtcaaac ggtttactgt
    15901 taggattgta aagctcaatg aattcgtcat tggcgtcggt ctggggtgcc gccggattag
    15961 gtaaaatctc tgaaagctgc ggcgccacca ggccgctgtc gtcacccggc aggttgctgc
    16021 cagccgtcac cacaacgctc ggcaccgcgc cggcagcttt ggctacagcc gtatttacaa
    16081 ccccgccggc actaatatcc agttggcaag gcaccagagg gtcaaggtcc gccagctgcc
    16141 agccgccggc cgtgcggtaa tacacgccct tgggatcttt gctgctactt gggacgtttg
    16201 ggatctggcc gctggctccg gagctccaag atacaaaatc ggcgggaacg gtggcgatca
    16261 gaccattggc actgcctaaa atttccaatg aaccgccgga atcgcccaaa cttaggctga
    16321 gtttgccggc cagttcagcg ccgcaagtat tctgagcaat gtcgcttaaa agcaaggcct
    16381 gcccgctgtc caatttagcg gcaggcaatt gctgggtgga actgctggcg ccggcagcct
    16441 gcggattgac attgttgaac tcatacagcc agtagccggt cacatcgatg gcggcagtgg
    16501 tgttgttttg gatgaccacg aactcatcac cggtgatttt gatttccgag attgccaaag
    16561 tttgcggtac ggtcatagct ggcggtgcgg cggcgtcggc caatggccgg gctaaaaata
    16621 acagtccgaa aagcataaga aatattacta tgcaacattt cttcatacaa tcaattatca
    16681 ggaaactctc agccaatgca agcagtagcg gattaatctg acaaaagcta tactaaaaat
    16741 atgttgaggt tgagtggcag tttgcggaac cggccggtct tgagcatgcg ctccggcggc
    16801 ccggtcgcca cggctgtgga accggtcatc aacccgcaca atctgaagat tttgggctgg
    16861 tggtgcaaag ctccaggtgg caaagagctg gtccttctgg ctgataacgt acgagagatc
    16921 atgcccaacg gcctggcggt caatgacgac gacgcgctga gcgctccgga agatctggtg
    16981 cgctataaag acatactgga gatccgcttc cagctgatag acaaaccggt caaaaccaag
    17041 cgccaaaaac tcggcaaggt cagtgatttc agctataacg acgggctgtt tgtgcagaag
    17101 ctgtatgtag cgcgcagcct ggtcaaggtt tttgccgccg aggatgtttt gttgattgac
    17161 cgtacgcaaa ttttggaagt taccgaccac tacatcttag tgcgcgacag cgaggtcaaa
    17221 gccacggagg aagagctggc cggcgcgctg gccaccgcct aatctcgctc acccatctgt
    17281 cagatgggct cgctcttcac tttgcactct aaactctcca ctaaattact tgactagtca
    17341 agtatttttc agagtgtctt ttatcagctg gtagctgaag ccctgctgca tcaggtactg
    17401 tttgagtttc acggggtctt tttggtagcg cggcagtttg gctttttttg cgataatagc
    17461 ttttaatcgt actccttcat cggtctctgc ctggtgtaaa accttgtccg ccagttccct
    17521 atttatgcct ttggcaaaaa gctcagagcg gatggcgcgg tcgcttttgc cgcgccggac
    17581 ctgcaattcg acgaaccagg cggcgaattt ggcgttgtcc aagtatctct tccggctaaa
    17641 ttctttcaca aaaatctttg tttgctcggg tccggctttt ttccggtata aataatcgcg
    17701 gaattcacgc tccgaatgcg gccggttcag cagccattcc agcgcccggg cccgcagttt
    17761 gccatcggcg gaaagctttt tgaatttttt ttcgtcggcg gcactgagat catcgccctg
    17821 ttttatccgg tacttggcca gctcgtccag actcaaagag aagctgtatt tttcatctac
    17881 gaagatgctg actcgctggg ggtttttgat ctgggccttg agggcggtaa ctttcatggt
    17941 tacggtcttt gcagtaccgg cagcgagcgg gcgtaaattt gctggcggac acttagcaat
    18001 ccgtggttgc cgcttgggta tacataataa gagtgcggca gctgcagctg gttgagcgcc
    18061 aggttgaggt cagacgagaa cttgggcggc acgattttgt ccttagcgcc gacgtggatt
    18121 tggatatatg cgtgggtctc aatcaaaaaa ttcatcggcg aggtgtagtt ccaaaaggtc
    18181 gggttgttga gcggtgtgcc gtattgcttg agcaccgctt tgcggatttt tagcgccacc
    18241 gggttggcat aatcggagcg cggcctgtag tcgccgtaca tgtcctgcgg gtaatccagc
    18301 ggcgtggata gcaaaattac gctgcggata tccggcgaca gtactgccgc ccgcagagct
    18361 atgtaagccc ccatgctatg gccccagaga ttgatatcgc cgccattgag accggtggtt
    18421 tttttgactg aagcgatcag actcatgata tcggtattgt aggccattga gtaataggcg
    18481 ccttccggct ggccggcgtg gatggacagg ccctggccgc gtaggtccgg cttgagcact
    18541 acaaaaccgt gctggctgta aaactccatg tccggcaaat aggccttttt ggtggaatat
    18601 gcccccggct ggatgtagcc atgaatcagg ataacaaccg ggtagccgcc ggccggcgcc
    18661 gggctggcgg gcgtagtcat cagtccgtac tcggtgaggt tgtcggccgg cacggcaaag
    18721 ctgaagtagc ggcggctgac gccggctgcc ggacttaact tggtaagctc tttgattggg
    18781 ccgccttgat aaatggccgt gccggccacc cgcaggtcca aactgacata cggctgattg
    18841 gtagaaatca gctgattgtt gccggcagcc ttggtggtaa acggctcgcc gacctcgctg
    18901 cctacaacat aaaccaacac caatactgcc agcgagcaaa caaaaatgag cgtagatttt
    18961 aacggcagtc ggtggagtct catacttagc ggcctatcct aagtataagt gccaacatca
    19021 acaccaacca gaaaatttca acataagctc cccatggccc ttccgaccac tgcacaaact
    19081 tgtggaatct tttaagctta gccattatac cttcgccttt gaaactgaca ttgcgcgctt
    19141 taatgataac cggcagccac attaaatcag gagcgatagc caacagcccg caggcaacaa
    19201 tcagccagcc ggcaactttt agaatcatca ccatcgcagt taagaatgcg gctgtcaaaa
    19261 gtaagtctat tgctacgact acgtggtaaa gattcggttt tggtattaga tctttatggt
    19321 cgaagtgtgg aatcgcatct gatacgaaat gcgataaaaa agccgctggc aaagcgattg
    19381 ctggttgctt gactatcaca gccaccaaca caccagtcgc ggcgtggttt aacccggtca
    19441 tgacattatt ttacaagaag ctattcggct tgggcggcgg tgagggtctt tttggcgatt
    19501 tcgtccaaaa ctttgggatt gtctttgagg tattgcttgg cggcttcgcg gccttgggcg
    19561 attttttggc cgccgtattc gtaccaggcg ccgtttttgt ctaccacgcc atggactgcc
    19621 gccaggtcaa tgacgtcgcc ctcatagctg ataccttggt tgtacatgat gtcaaactca
    19681 gcttcacgga atggcggcgc cactttgttt ttgacgactt tgacccgcac gtgattgccg
    19741 atgacggcat cgccggattt tatctggcta agacgccgga tatccatgcg tacgctggcg
    19801 tagaatttca gagcgttgcc gccggtggtg gtttccgggt tgccgaacat gacgccgatt
    19861 ttcatgcgca actggttgac aaaaaccacc gtgcacttgg tgcggctgat gacgccggtg
    19921 agcttgcgca tggcctggct catcaaccgg gcctgcaggc ccatttgggc gtcgcccata
    19981 tcgccctcta tctcagcctg cggtaccaag gcggcgacgg aatctaccac cacgatatcc
    20041 acagcgttgg agcgtaccaa ggcttctacc acttccaggg cctgttcgcc gctgtccggc
    20101 tggctgatca aaagattatc aatgttgacg cccaggcgtt tggcgtaagc cggatccagg
    20161 gcgtgctcgg catccacgta agcggcagtg ccgccggctt tttggacctc ggccacggcg
    20221 tgcagacaaa ccgtggtttt gccggaactt tccggcccgt aaatttcaat gatacggccc
    20281 ttgggtatgc cgccgcccag cgccaggtcc aggctgatgg agccgctggg gatggtctca
    20341 atatctattt tgtgggcctc gcccaggcgc ataattgagc ctttgccaaa ctgcttttct
    20401 atctggtcaa ccgccaactg cagggcctgg gccttgccgc tgccggcggc tgttggcgca
    20461 ctatctttct tgattttggt cttatcggcc atcgcgactc ccatctagta ttctcctcta
    20521 ttatacaaga gactataccg cccggccgga tgtttgaaaa ttgctgctgc ttaaaaactt
    20581 aaaattccct gaaaattact tgactagtta agtaattcag tggaaagtga agagtggaga
    20641 gcgaaacccc tcacttttat ccaggctcga ggcgaacctg agtagatgtt gtttaaaaga
    20701 ggtgccctgg ggcgtgaaag tatgaatcaa ctatttggag attcattctt gagcgagcct
    20761 gttttaaaca acagctacat cgagtgagcc gaatgtctgg gaaaagtcag gggtgagcta
    20821 aggtttggtc aggacttcgc gccaggacag gatattgaag ccggcagtca gcggatagct
    20881 gggtggcggc gaatacagca tattgtaatc gtaactggtg acggtgtgct caataccgga
    20941 gacccaaccg ttaaccgggt cgttggcggc gtcgccgcag gcactaccta ccagccagtt
    21001 ccaggtccag gtctgacggg tagaaaccga accgtaaaat atcagctgct ggttggagtt
    21061 gacccagccg tgggtacaac gagagctggc tgtgcggtaa taaaccggat attcaacatt
    21121 gccgttttcg gacaatattg ccgcgtcaat ctcatagttg aaactgccgc tggccggcgg
    21181 cgcgtacggt gcaaccagca catcgccctc agccaccaga ccaattgcat cctggccgtt
    21241 cttggtggag taaagcaggt catcggagat aacgatgtcg gtattgttgc tgctggccag
    21301 gcggccggag ccgatagtca cccggccatg gaaggtcggg ttggagcgaa tccaaacgtt
    21361 atcctcggca aaaatcacgc cgctggaggg aacgacaatg ccgctggcca ccagctgagt
    21421 agtcagagcg ctggaatagg tcgcggcccg gtcattctcg ccattgacgt tatagagatc
    21481 gtaagtgccg ttggtattca gattgatcag atagcctttg gtcagactga aactggaact
    21541 gcgctgcggc agataggccg ctgtgcgggt gctgggagtt tgggtgcaag cgttggcttg
    21601 gttggccaga ctggcggtgg agctatagga ggcaaaagct atctttttca ttgaacacag
    21661 gctggtattg acctggttaa aatcaacggc gctgactggg aacagccagt tggttttgga
    21721 gcgggtgttg cagttgaccg gcgaggttac actgctgctg caccaaacac caggttcgga
    21781 agtatggcca tcgccgccca gggcggtcga gggtacgtaa gtggtgttgg cgctggatac
    21841 agtatcggtg ttcggcccgt ccattcttat accctgattg gagccgatcg ggccgtcggc
    21901 cgtctcgtcg ttgccgaacc acagcgccga atcgcccagc acaccataac tggcgaacga
    21961 cggctcgcca atctgagcct ggatggtacg gacgtatttg ctgtttttaa cctggccggt
    22021 agactgtacc gtggccacgg tggagcctcc gccctggggc ttaatccaaa gcgtgaaagt
    22081 gccctcatta acggcattac tgtcaatgta gttatgcacg tacgggccat atcccagcgt
    22141 cgggtcgggt gtagtcggcg tagattggcc gtctttaaaa tccgtcgggt tatggcttag
    22201 atgccacaga taataattga tgccggcttc agcgatgttg aatgcctgct ggctctggac
    22261 attgttgtta actatcgaga gattggtgtc aatcacttct accagagccg ccgcgaggat
    22321 gatcgccacc acgatgaaac tcagtattgt cggcaaaatg aaggcttcct gcgactgggc
    22381 gaaacgttta aaaaatctca taggttagtc ttccggttac gcaaactgat ctgcagcgat
    22441 atctcctggt tgccggattg ggtgggaaaa tcggccggca ccgccaggtt aatctggatg
    22501 cctttgatgc tgttctcgtc gctgatcggc aggctcagga cgttaaatga agaatcaaga
    22561 tagttgaaca ggttcaggcc gggcagtttg taatagttat caatgatagt ataggtcact
    22621 ttttggccgg tgataggcgt accgatgggc ggattggcgg tcatcggcgt gacatcggca
    22681 tacagcgcgg tggagttggc attgaggtag tagtggattt ctgatacata ggtgtcgttg
    22741 ggcgagaagt aagcataaac cgtcaggtcg ttagcggact cggagacgat atcggtcaga
    22801 ccgcgcagaa cattggcgat acgctgggat tgggaagcca gattggtgaa ttccgagccg
    22861 tcggcctgta agccaaagta ctggttaaga agggtgctga aaatgctgta ggccgtcagc
    22921 gacactaaaa aggctatggt tatggcgatg gtcagctcca caatcgtgaa accggcccgg
    22981 cgggacgttc gccgcatctc tttaaggcgt gacactcagg cctcccatct gcgggctgaa
    23041 cggcaagctc acactgccgc tgctggtgcc gacgaccagt tgggcattac cggtaccaat
    23101 acccgtaagg tttaccgaac agttaagttg gaccccggcg ctggcaccgg tgcaggtcgc
    23161 cgtgtaggtg ttgccgcctt gaacaaaccg tacactgctg ctgcagccgg aaccgctggt
    23221 accgcaagat aacttctggc cgctgacggt gaactggaaa ttgttgatag tgccaccgga
    23281 caggctgaca tcgtacgggc taaggctgaa aatccgcggc agattagcgt tagtgctgag
    23341 catcaaacgt acctcttgca gatagctggt gccagcgtag ctgtaggtgg tggtcggcgg
    23401 gttagtcgga tcatatgtcg gcaccgttat tggcgccagt gagttactgc cgccataagg
    23461 taccgccgct atcaagtaat actggg
//
LOCUS       DASPTF010000072         6246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_111716, whole genome shotgun sequence.
ACCESSION   DASPTF010000072 DASPTF010000000
VERSION     DASPTF010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111716"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VEH48_02455"
     CDS             <1..635
                     /locus_tag="VEH48_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXY18253.1"
                     /translation="TPPPVPPPSTATVPPPPVSQPATPTLPPGVKREDCELLEPDEEI
                     VMVVHRHPIGIIMIYIEALVGVGAVVALLILLSPSVLQNLSGQAYTLMMGIIILAIGL
                     VAMILLLATYIYRQSRLVVSDKSLIQVIQRGLFIRKISRLSMSNVQDVQADHEGFLAT
                     IFNFGTLTVETAGEEDNFIFPLCPTPDTYADRVLEARQRYVHSEEGKQGL"
     gene            complement(936..1244)
                     /locus_tag="VEH48_02460"
     CDS             complement(936..1244)
                     /locus_tag="VEH48_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18254.1"
                     /translation="MNKSPNPGFKAILPWLRKHQKQMTSHAVFAAVLLVLLAYVFMVW
                     RIGQLATANPGPDQLSSSSSGQTPTVDKSAIDQIQKLEQSNTNVHALFDNARNNPFQE
                     "
     gene            complement(1246..1917)
                     /locus_tag="VEH48_02465"
     CDS             complement(1246..1917)
                     /locus_tag="VEH48_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18255.1"
                     /translation="MKFLNFKLDSQHLRFVLLGLLGLAGVVFIGIMFLGLSMLSAKSQ
                     KMVNLKVQSSAVDNQLNSLALAKKQIKQYGYFKTVASEVIPNDKDQAAAVLQINQFAS
                     QAGFLLQTITFPASNLGGSSSASGQVSAQTASPASLLSQALPVSGINGLYSVQLTITP
                     QTDVNLPADEQVTYSKLLDFLSLIENNQRTAQISQIVIKPVYNGDGSFRAFDFTLEIN
                     IFIRP"
     gene            complement(1917..2513)
                     /locus_tag="VEH48_02470"
     CDS             complement(1917..2513)
                     /locus_tag="VEH48_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18256.1"
                     /translation="MINLLPPERAAQIRYGRINSLLRRWLAAAGIAILILVVIIASGL
                     VYTNQQSKGLLADIAATNQELSDQNLKQIQADAATISGDVKVINQVLNKEVRVSDLMT
                     AIGQYMPAGAILSSLTLGSVTGAIDLTASTVDYSSAAQIAVNLSDPASQLFSKVDIVS
                     ISCQNSHPGTSYACAAVYKALFSKAAASNFVNPVKGGS"
     gene            complement(2510..3580)
                     /gene="pilM"
                     /locus_tag="VEH48_02475"
     CDS             complement(2510..3580)
                     /gene="pilM"
                     /locus_tag="VEH48_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HXY18257.1"
                     /translation="MNPLSFYRDQALFGLDIGHSSLKVMQLDSRAGKTPRVLGYGVAP
                     YLPKAIKAGIITDHKALHDSLFQLLDKDLVGEIDTRRVACTIPTALTFSRSMKLPPMA
                     RSGLAEAVNLEAEQYIPIPIANLYLDYEISHEDAQGIELLMVATPRNIIDSHVAFLEK
                     VGLDPVALEPTMNSAGRLFALTDSSHTQPSILIDFGSVAVDVAVFDKTMFVNTTVQGG
                     GDDITKLIAQQLKVTPAKAQDLKNKYGLGSSPKQKEIKQAIKPLLDNLVHEIQKIVRY
                     YNDHQTQGPRKIAQIVAIGGGANIPGLNDFLSGELHLPARKLEPWKAIDFGDLVMPSE
                     IDRLMYTTVAGAASLNPKEIFA"
     gene            complement(3699..4151)
                     /locus_tag="VEH48_02480"
     CDS             complement(3699..4151)
                     /locus_tag="VEH48_02480"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXY18258.1"
                     /translation="MLSFKKLRNQGFTIVELLIVIVVIGILATLVITTYNGVQSKARD
                     TKRKTDINALQGQVEAYNTNAGFYPTLADMNDSAWRSTNMKGLDPSALNDPQNPNPTS
                     TLCATTTAHCYVYAVTPSGCDNSTTDCTSYTLSASLEDGTTYSKTGSD"
     gene            complement(4278..5534)
                     /locus_tag="VEH48_02485"
     CDS             complement(4278..5534)
                     /locus_tag="VEH48_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXY18259.1"
                     /translation="MKSDNSNTDTRQLPQFSYTTMVGSKEITGQIAAPTREAAITSLH
                     QQNIKPLSVHEVKAKKGLLAKSFGRGKVKKRDLVIFTRELSTMVSAGVPLPRALGTLG
                     DQAENKYFQEVVRGIDHQIEGGAPLSDAMAKYPNVFSEVYVNMVAAGEAGGILDDILK
                     RLATQVEKDETIRKKIRAAMAYPVVIMTITVIAFFGIMIFILPKIAKIIKDLAGPNAQ
                     LPVYTRVLMHISNFMQHNFIIIFIVLAVLIYSFKHYISTEKGKYRWHGFLLRMPIFGP
                     VVAKIVVARFSRTFASLMGSGVSILEALDVTGKAVGNKVIQKELAEIALNVKNGQPLG
                     KQLMSAKFFPPIVGQMMSVGEETGKIDQVLVKVADFYEEEVDTVIDSLASIIEPVMII
                     VLGAIVGVIAASVMGPIANLSKQISG"
     gene            complement(5524..>6246)
                     /locus_tag="VEH48_02490"
     CDS             complement(5524..>6246)
                     /locus_tag="VEH48_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXY18260.1"
                     /translation="VQVNPVAGMTFANGLRALLRQDPNIIMVGEIRDNETANLAVQAA
                     LTGHLVFSTLHTNNAATCLPRLLDMGIEPFLIASTVRAIVAQRLVRRLVPEAMEAFTP
                     DEPTIKRIETAFGISGAGGMKQIHELEMQAINGNIGQFGPSGKTAEAMEPSTSEQTIN
                     RLWRIGGSSGTSSFHGRIGIYEVLTLSPQIQKMIIGSSSSDDIQNQAIQEGMITMQTD
                     GFIKALRGQTTLEEVMRVTSEK"
BASE COUNT         1308 a         1591 c         1804 g         1543 t
ORIGIN      
        1 tcacaccccc gccggtgcct ccacctagta ctgctactgt accaccaccg ccggtctcac
       61 agccggctac ccctacctta ccgccgggag tcaagcggga agactgcgag ctgctggagc
      121 ctgacgaaga gatagtgatg gtcgtccacc gccatcctat cggcatcatc atgatttata
      181 ttgaggccct ggtcggtgtc ggcgcggttg tggcactgct gatcctgtta tcgcccagcg
      241 tgctgcaaaa cctgtcaggc caagcctaca ctctgatgat gggcatcatt attctggcca
      301 tcggtctggt ggcgatgatc ctgttgctgg cgacttacat ttaccgtcag agccgtctgg
      361 ttgtcagcga caaaagcctg atccaagtta tccaaagagg gctgtttatc cgcaagatct
      421 caaggttatc aatgagcaat gtccaggacg tccaggccga ccacgaaggc tttttggcga
      481 cgatttttaa ctttggcacg ctgaccgttg aaaccgccgg cgaggaggac aactttattt
      541 ttccgctgtg tccgacaccg gatacttatg ccgaccgagt cttggaagcg cggcagcgtt
      601 acgtgcacag cgaagaaggc aagcagggtc tatagtcaaa atacttaaca agtcaagcaa
      661 tttagggttg tagggttata gggtgaaagg gttatagggt atcagctgca ccccttcaac
      721 cctacggcac tctcaccctg cttattcttg atgagcaaga cccctaactt ttatccagac
      781 tcgaggcgaa cccgagtaga tgttgtttaa aagaggcgtc ctggggcgca aaagtatgaa
      841 tcaactattt ggagattcat tcttgagcga gcctgtttta aacaacagct acatcgagtg
      901 agccgaatgt ctgggaaaag ttaggggttg agcggctatt cttggaaggg gttgttgcgg
      961 gcattatcaa acaaagcgtg gacattggta ttgctctgct ccagtttctg gatctggtca
     1021 attgcggatt tgtctacggt gggtgtttgg ccgctgctgc ttgaggagag ttgatctggt
     1081 ccggggttgg ctgtggccaa ctggccgatg cgccagacca tgaaaacata ggccaacagt
     1141 accagcagca ccgcggcaaa aacggcatgg gatgtcatct gtttttggtg cttgcgcagc
     1201 cacggcagga tcgctttgaa acccggattg gggcttttgt tcatcttacg gccttataaa
     1261 tatattaatt tccagagtga agtcaaaggc ccggaacgag ccgtcgccat tataaaccgg
     1321 cttgatgacg atttgactga tttgggcggt gcgctgattg ttttctataa ggctgagaaa
     1381 gtccagcagc ttactataag tcacctgttc gtcagccggc agattgacgt cggtctgcgg
     1441 cgtgatggtc aactggacgc tgtaaagccc gttgatgccg ctgaccggca gggcttgact
     1501 gagcagcgaa gcgggcgagg cagtttgggc gctgacctgg ccgctggcgg aagagctacc
     1561 cccaagatta ctggcgggga aagtgatagt ctgcagtaaa aaaccggctt ggctggcaaa
     1621 ctggttgatc tgcagaacgg ccgccgcctg atctttatca ttagggatca cttcgctggc
     1681 gacagtcttg aagtatccgt attgcttgat ctgtttttta gccagcgcca agctgtttaa
     1741 ctggttgtct acggccgagc tctgcacttt aaggttgacc attttctggc tcttggcgct
     1801 gagcattgac aatcccaaaa acatgatgcc aatgaaaacg actccggcca ggccgagcag
     1861 tcccagcagg acgaacctca ggtgctggct gtccagtttg aagttcagga acttcactag
     1921 ctgccgcctt tcaccggatt gacgaaattg ctggcggcgg ccttgctgaa tagggccttg
     1981 taaactgccg cgcaggcgta agacgtacct ggatggctat tctggcagct gatactgacg
     2041 atgtccactt tggaaaagag ctggctggcc ggatcgctga ggttgacggc gatctgggcg
     2101 gctgaggaat aatcaaccgt gctggcggtc aggtcgatgg cgccggtgac actgcccagt
     2161 gtcagcgaac ttaagatagc gccagctggc atgtattggc cgatggctgt catcagatcg
     2221 gaaacccgga cctctttatt aagcacttgg ttaatgactt tcacatcgcc gctgatagtc
     2281 gcggcgtcgg cttgtatctg cttaaggttc tggtcgctta actcctggtt tgtcgccgct
     2341 atgtcggcca acaagccttt ggactgctgg ttggtataca ccaggccgct agcgattatg
     2401 accactagta taaggatagc tatgccggct gcagccagcc agcgccgcaa caggctgttg
     2461 atacggccgt agcggatctg ggcggctcgt tccggtggca acaggttgat catgcgaaaa
     2521 tctccttggg gttcaaactg gcggcaccgg cgacagtggt gtacattaag cggtcgattt
     2581 cgctgggcat gaccagatcg ccgaaatcaa tagccttcca gggttctaat ttgcgcgccg
     2641 gcaggtggag ttcaccggag agaaaatcgt tcagcccagg gatattggcg ccgccgccga
     2701 tagcgacaat ctgggctatt ttacgtggtc cctgggtctg gtggtcgtta taatagcgga
     2761 cgatcttctg gatttcgtgc accagattat ccagcaaagg cttgattgcc tgcttgatct
     2821 ccttctgttt ggggctggaa ccgagtccgt acttgttctt gaggtcctgg gctttggcag
     2881 gcgtgacttt aagctgctgg gcaatcagtt tagtgatgtc gtcaccgccg ccttggacgg
     2941 tggtgttgac gaacattgtc ttatcaaaga ctgcgacatc gacggccacc gatccgaagt
     3001 caattaaaat tgacggctgg gtgtgcgacg aatcggtcag ggcgaacagc cggccggcgg
     3061 aattcatggt cggctccagc gctaccgggt ccaggccgac tttttccaga aatgccacat
     3121 ggctgtcaat gatgttcctg ggcgtggcga ccatcagcag ctctataccc tgcgcgtctt
     3181 cgtgggagat ctcatagtcc aggtagagat tagctatagg gatcggtata tattgttcgg
     3241 cttccagatt tacggcttcg gccagaccgg agcgcgccat cggcggcaac ttcatcgacc
     3301 ggctgaaagt cagcgccgtc ggaatggtgc aggccaccct ccgggtgtca atctcgccca
     3361 ccaggtcttt atccagcagc tggaacaagc tgtcgtgcag ggctttatga tcagtgatga
     3421 tgccggcctt gatagccttg ggaagataag gggctacgcc gtagcccagg acccgggggg
     3481 ttttacccgc ccggctgtcc agctgcatga ctttcaagct ggagtggccg atgtccaggc
     3541 caaacagcgc ctgatcgcgg tagaaactga gcggattcat ctgttgatat cggttatcct
     3601 tacctgcaaa aagtatagca ttaaagggtt acaaagcctt tcttaagtaa gaaaaaaccc
     3661 ggagcttaag ccgggttttt tctttagctt gggctggatc agtcgctgcc ggtcttggaa
     3721 taggtagtgc cgtcttccag tgacgcgctc agcgtgtaac tggtgcagtc ggtggttgag
     3781 ttgtcgcagc ctgacggggt taccgcgtag acgtagcagt gagccgtagt ggtggcgcac
     3841 agcgtagacg tcgggttcgg gttctgcgga tcgttcagcg ccgaaggatc gagtcctttc
     3901 atgttagtac tgcgccaagc ggagtcattc atgtcggcca gcgtcgggta aaagccggcg
     3961 ttggtgttgt aggcctccac ctgtccctgg agagcgttaa tgtcggtctt gcgcttggtg
     4021 tcgcgggcct tggactggac accgttgtag gtcgtaatga ccagcgtggc cagaataccg
     4081 atcaccacga ttacgatcag aagttccacg atggtgaaac cctggttcct taacttttta
     4141 aatgagagca tattgattcc cacccttctt aggttaatgt atttctttta tttggcaaac
     4201 gagcgaacgt tccatgagca tgattgtata aacacaagca tcatcttgtc aagcagaggt
     4261 gtggacagat agatgaatta accgctgatt tgcttactca agttggcaat cgggcccatg
     4321 actgaagcgg cgatcactcc gacgatagcg cccagtacga taatcattac cggttcaatg
     4381 atgctggcca gactgtcaat gacggtatcc acctcttctt catagaagtc ggctaccttg
     4441 accagtacct ggtcaatctt gcctgtttcc tcgcccacgg acatcatctg gccgacgatt
     4501 ggagggaaga acttggcgct catcagctgc ttgcccagcg gctgtccgtt tttgacgtta
     4561 agggcgattt cggccagctc tttctggata accttgttgc ccacggcttt gccggtgacg
     4621 tccaaggcct ccaggatgct gacgccggaa cccatcagcg aagcaaaggt ccgcgaaaaa
     4681 cgagccacca cgattttagc caccaccggt ccgaaaatcg gcatgcgcag caaaaagccg
     4741 tgccagcggt acttaccctt ttcggtgctg atgtagtgct tgaaactgta tatcaggacg
     4801 gccagaacta taaaaatgat tatgaagttg tgctgcatga agttgctgat atgcatcagt
     4861 acgcgggtgt aaaccggcag ctgggcgttg gggccggcca gatctttgat gatcttggcg
     4921 attttgggca ggatgaagat catgatgcca aaaaaagcga tgaccgtaat agtcattatg
     4981 actaccggat aggccatggc cgcccggatt tttttgcgga tggtttcgtc tttttccacc
     5041 tgggtggcca gacgtttcag gatatcgtct aagataccgc cggcttctcc ggctgccacc
     5101 atgttcacgt aaacctcact gaagacattg ggatacttgg ccatggcgtc ggatagcggc
     5161 gcgccgcctt ctatctggtg gtcaataccg cgcaccacct cctggaaata tttgttctcg
     5221 gcttggtcgc ccagggtgcc gagcgcccgc ggcaggggta cgccggcgct gaccatggtg
     5281 gacagttcgc gggtgaaaat caccaggtca cgttttttaa ctttgcccct gccaaacgat
     5341 ttagccaaca ggcctttctt ggctttgact tcgtggacgc tcagcggctt gatgttctgc
     5401 tggtgcaggg aggtgatagc ggcctcgcgg gtcggcgcgg cgatctggcc ggtgatttcc
     5461 ttactgccga ccatagtggt atagctgaac tgcggcagct gtctggtgtc ggtattacta
     5521 ttgtcacttt tcactggtaa ccctcatgac ttcttccaat gtggtctggc cgcgcagggc
     5581 tttgataaag ccgtcggtct gcatggttat catgccttcc tggatggcct ggttctggat
     5641 gtcgtcactg ctgctgctgc cgataatcat tttttggatt tgcgggctga gcgtcaaaac
     5701 ttcatagatg ccgatacggc cgtgaaaaga gctggttccg ctgctgccgc cgattcgcca
     5761 cagccggttg atagtctgct cgctggtaga aggttccatg gcctcggcgg tcttgccgct
     5821 ggggccgaat tggccgatgt tgccattgat ggcttgcatt tccagctcat gaatctgttt
     5881 catgccgccg gcgccgctga taccaaaggc tgtctctatg cgtttgatag tcggctcgtc
     5941 gggcgtgaaa gcttccatgg cctccggtac cagccgccgc accaggcgct gggcgactat
     6001 cgcccggacg gtactggcga tcaggaaagg ctcaatgccc atgtccagaa gccgcggcag
     6061 gcaggtagcg gcattattgg tatgtagggt ggaaaatacc agatgaccgg tcagtgccgc
     6121 ctggaccgct aggttagcgg tctcgttgtc gcggatctcg ccgaccatga tgatgttggg
     6181 gtcctggcgc agcagcgccc gcaggccgtt ggcgaaagtc atgccggcca ccggattgac
     6241 ttggac
//
LOCUS       DASPTF010000073         4891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1845430, whole genome shotgun sequence.
ACCESSION   DASPTF010000073 DASPTF010000000
VERSION     DASPTF010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845430"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..794
                     /locus_tag="VEH48_02495"
     CDS             102..794
                     /locus_tag="VEH48_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXY18261.1"
                     /translation="MDKRFLSILGAIIIIFVGIFVFSQHSNNNSSSSSNSSQQPTSHI
                     EGQGQAGVTLVEYGDYECPICGLYYPVVKQVETQFSSQIYFQFRNLPLTSIHKNAFAG
                     ARAAEAAGLQGKYWQMHDALYDNQDPNGTSGWVASDNPLSFFTTFAQQIGLNVNQFKT
                     DYNSDKVNNAINADLAAFAKTGQEQATPTFFLDGKYISNLDLSDSATGQPSVQKFAQV
                     INAEIAAKKKNT"
     gene            1006..2370
                     /locus_tag="VEH48_02500"
     CDS             1006..2370
                     /locus_tag="VEH48_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HXY18262.1"
                     /translation="MAKKHPLEEFTKSEVDALDATLVKTARAAENAEIKTVKKTREYW
                     NVLGPGLTTGASDDDPSGIATYSQTGAQYGFQLLWLAPLTFPLMSVVQEMCARIGIVT
                     GRGLAGNIRAHFSKKVLYACTLLLFAANSFNIGADLGAMAKGIQLLRPGLDFSVLVIG
                     FALLCVLLQIFLPYARYARYLKWLALILLAYVVSAIMAHLHWGEVLHQTYSPSIKWSK
                     DQLLLICAILGTTISPYLFFWQTSQEVEEQIVEGHTTIASRQGAHPKEIRSMRVDVWS
                     GMFLSQIVMFFIIAACGALLFGNGITNITSAAQAAEALRPFAGNLTYWLFAIGIIGTG
                     LLAIPVLAGSSSYATAESFKWREGLYRKFRQAHAFYGILILSVFIGLGINFLNIDPIK
                     ALIYSAVANGVVAPLVLALIVILSSNHGIMGDWVNKRYVTWLGWGVTGLMAVAGLAAI
                     YSLF"
     gene            2544..4304
                     /locus_tag="VEH48_02505"
     CDS             2544..4304
                     /locus_tag="VEH48_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXY18263.1"
                     /translation="MDTVFSGLSLIIVVAALMALVMRLINQPLIIGHILTGIIVGPAV
                     LHIAKAPGTLALFSDLGIALLLFIIGLGLNPRVIKEVGRTASIVGGSQVLVITVLGFL
                     AAKFFGLGNNASMFVGFSLAISSTIIILKLISDKKEQGRLYGKIAVSVSLVQDIIAIA
                     LVVFTSASSHQSIAYGSLLSLLIKALVAGWVIYAVSFRILPRFHHVIAESQEFLFLFA
                     IAWGLGSAALFQKIGLSTEIGALLAGICLAPLPSAPEIGARLRPLRDFFLVVFFIALG
                     SKLTLSSIGAHLGLIIAGVVIAAILKPLVAMSVLGFLGYTKRTSFKAAISLAQVSEFS
                     IVLIIIALNKGLINSDIVSAIVFMTLISIAASTYMITFSDQLFGVFENYLGMFERRKA
                     QSELKPEASNELVLFGYQRGGHEFINLFKKLKKKYVVIDYDPEVIDVIEERKLNYLYG
                     DATDVELLEEAGVEHAKLIVSTIPDHQTNMVLLNYLKTKNPQAIVIIHADDPYEAAKL
                     YEAGASYVILPHYLGSEKVSEFIAKSGLSKSTFRKQRVRHLDYLEKHYGALEKLNEIN
                     ERKLGRSIVKRVTSLSKAKA"
     gene            complement(4319..>4891)
                     /locus_tag="VEH48_02510"
     CDS             complement(4319..>4891)
                     /locus_tag="VEH48_02510"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HXY18264.1"
                     /translation="GYTGLETAMIKKSNLNFAIAVFLILLAVSLRLLPHPANFAPVAA
                     VAIFGGAVLPRRLAVWVPFVAMALSDLVIGFYNIMPVTWGCYVLIALASSYWLRKPGF
                     LRGISLTLSSSLFFFIVTNFAVWLWSSMYAHTWSGLAQCYTMALPFFRNTALSDLFYT
                     ATLFAVYVLATKAGVRLLAVGAKSAGVKNT"
BASE COUNT         1152 a         1358 c         1231 g         1150 t
ORIGIN      
        1 taaaacgaaa agtgcagcca tcataagttt aattttacct taagtaccga gaaacccgcg
       61 gcgtggtatg attagaatat aaaggagtct caagtcaaga tatggataag cggtttttat
      121 caatcctagg ggcgattatc atcatctttg tcggtatttt cgtcttcagc cagcactcta
      181 ataataattc cagcagctct tctaacagtt cccagcaacc caccagccac attgaaggcc
      241 aaggccaagc cggtgtaact ctggtggagt atggcgatta cgaatgtccg atttgcggcc
      301 tgtattaccc cgtggtgaag caggtggaga cacagttcag cagccagatt tacttccagt
      361 tccgcaatct gccgctgacc agcatccaca aaaacgcctt tgccggcgcc cgggccgccg
      421 aggccgccgg actgcagggc aaatattggc agatgcacga cgcgctctac gataaccagg
      481 atcctaacgg gaccagcggc tgggtagcca gcgataatcc gctttccttc ttcacgactt
      541 ttgcccagca aatcggcctg aacgtcaatc aatttaaaac tgactacaac agcgacaagg
      601 ttaacaacgc catcaacgcc gacctggcgg cctttgccaa aaccggccag gaacaggcca
      661 cgccaacctt tttcctggac ggcaaatata tttccaacct ggatctctcc gatagcgcca
      721 ccggccagcc aagcgtccaa aaatttgctc aggtcataaa cgccgaaatc gccgccaaaa
      781 agaaaaatac ttaacaagtt aagtatttta tcaattgacc tgattgacct aatttctaat
      841 taacctaagt aatgactaaa ttactaaatc ccaagctgtt tgggtattga gtattaatta
      901 ttaggattta attagtcatt tagattaatt agtgcaatta ggaatttatt gtgtttatcc
      961 ctggttggct ataccataag tgctaaagcg atcccgcaaa aagccatggc aaaaaaacac
     1021 ccccttgaag aattcaccaa aagcgaagtt gatgcgcttg atgcgacttt ggtaaaaacc
     1081 gcccgcgcgg ccgagaatgc cgaaataaaa accgtcaaaa aaacccgtga gtattggaac
     1141 gtcttagggc cgggtttgac caccggcgcc tccgacgacg acccctccgg catcgcgact
     1201 tattctcaga ccggcgccca gtacggtttc cagctactgt ggctggcgcc gcttacgttc
     1261 cctctaatgt cggtagtgca ggagatgtgc gcccgtatcg gcatcgtgac cggccgcggc
     1321 ctagccggta acatccgcgc ccatttttcc aaaaaagtcc tctatgcctg caccctgctg
     1381 ctatttgccg ccaactcttt caacatcggt gccgacctgg gagcgatggc caaggggatc
     1441 cagttgctaa ggcccggctt ggatttcagc gttctggtga ttggtttcgc tctgctgtgc
     1501 gtgctgctgc aaattttcct gccctatgcc cggtatgccc gttacctcaa atggctggcg
     1561 ctgattctgc tggcctacgt tgtatcggca atcatggccc acctgcattg gggtgaggta
     1621 ctgcaccaga cctactcgcc atctatcaaa tggtcaaaag accagctgtt gctgatctgc
     1681 gccatcctgg gtacgacaat ttcgccgtat ctattctttt ggcagacctc acaagaagtt
     1741 gaagaacaga ttgtggaagg ccataccacc atagcctcgc gccaaggtgc ccaccctaaa
     1801 gagatccgct ccatgcgcgt tgacgtctgg agcggcatgt tcctttcgca aattgtgatg
     1861 ttctttatca tcgcggcctg cggcgcgttg ttgttcggca acggtatcac caacatcaca
     1921 tcggcagctc aggccgccga ggcgctgcga ccgttcgccg gcaacctgac ctactggttg
     1981 tttgctatcg gcatcatcgg taccggcctc ctggccatcc cggtgctggc cggttccagc
     2041 agctacgcca ccgccgaaag ttttaaatgg cgcgagggct tgtatcgcaa attccgccag
     2101 gctcacgctt tttacggaat cctgatactt tcggtattca tcggcttggg tatcaatttt
     2161 ctgaacattg accctatcaa agccttgata tattcggctg tggccaatgg tgtggtggcg
     2221 ccgctggtgc tggctttgat cgtcatcctc agcagcaacc atggcatcat gggtgactgg
     2281 gtcaacaaac gttatgtaac ttggctgggc tggggagtga ccgggctgat ggccgtcgcc
     2341 ggcttggccg ctatctactc actcttttaa agcgcctacg ctatcgctcc ggcttataag
     2401 acaccccttc ggggtttccc atcgcgccgg gcaaaaagtg cgatttttgc tcgggctgcc
     2461 cttcccgagg aaattacttg acttatcaag tattttcatc tctttaagct tgaggatttt
     2521 cagggtagaa ttaagtttga tagatggata ctgttttttc cggattgagc ctgataattg
     2581 tcgtcgccgc gctaatggca ctggtaatgc ggctgattaa ccagccgctg ataatcggcc
     2641 acatcttaac cggtatcatc gtcggcccgg cggtgctgca cattgccaaa gcgccgggta
     2701 ccctggcgct gttttccgac ctgggcatcg ccctgctgtt gttcatcatc ggcctgggtt
     2761 tgaatccgcg ggtcatcaaa gaggtcggac ggacagcgtc aatcgttggc ggcagccagg
     2821 tgctggtcat aactgtattg ggctttttgg cggctaaatt cttcggcttg ggcaacaacg
     2881 ccagcatgtt tgttggtttc agcctggcca tctccagcac cataatcatc ttgaagctca
     2941 tcagcgacaa gaaagagcaa gggcggctgt acggcaagat cgccgtcagc gtttcgctgg
     3001 tgcaggacat tatcgccatc gctttggtgg tgttcacctc ggccagcagc caccaatcaa
     3061 tcgcttatgg ctcgctgtta tcactgctta tcaaggctct ggttgccggc tgggtgattt
     3121 acgccgtcag tttccggatc ctgccgcgct tccaccacgt tatcgccgaa agccaggagt
     3181 tcttattctt gttcgccatc gcctggggtc tgggcagcgc cgcgcttttc caaaaaatcg
     3241 gcttgtctac ggagatcggc gcgctgctgg ccggcatctg tctggcgccg ctgccgtcag
     3301 cgccggaaat cggcgcccga ctgcggccgc tgcgcgattt tttcctggtt gttttcttta
     3361 tcgccttggg ctctaaactg acgttatcaa gcattggcgc acacttgggg ctgattatcg
     3421 ccggcgtggt catcgccgct atcctaaaac cgctagtggc catgagcgtg ctggggtttt
     3481 tgggctacac caaacgcacc agcttcaagg ccgccatatc gctggctcag gtcagcgaat
     3541 tctcgatcgt gctgatcatc atcgccctca acaaaggcct gatcaatagc gacatcgtct
     3601 cggccatcgt cttcatgacg ctgatcagca tcgccgcctc cacttatatg attaccttca
     3661 gcgaccagct attcggcgtt tttgaaaact acctggggat gtttgaacgc cgcaaagccc
     3721 aaagcgagct taagcctgag gccagcaacg agctggtgct gttcggctac cagcgcggcg
     3781 gtcacgagtt catcaacctg tttaaaaaac tcaagaaaaa atacgtggtg atagattacg
     3841 acccggaggt catagatgtt attgaggaac gcaagctcaa ttatctgtac ggcgacgcca
     3901 ccgacgtgga gctgctggaa gaggccggcg tggagcacgc caagctgata gtttcaacca
     3961 ttcccgacca ccagaccaat atggtgctac tgaattatct caagaccaag aacccccagg
     4021 cgattgttat catccacgcc gacgaccctt acgaggcagc caaactgtac gaggccggcg
     4081 ccagctacgt gatcctgccg cactacctgg gcagcgaaaa agtcagcgag ttcatagcca
     4141 aatcaggcct aagcaaatct actttccgca aacagcgcgt gcgccatctg gactatctag
     4201 agaaacacta cggcgctttg gaaaaactca acgagatcaa cgaacgcaaa ctcggccgct
     4261 ctatcgtcaa gcgtgtgact tctttgagca aagccaaggc ttaaaaatac ttgactagtc
     4321 aagtattttt aacgccagcc gacttggcgc ccacagccag taacctcacc cctgccttag
     4381 tcgccagcac gtaaacggca aacaaagtgg ctgtgtaaaa caggtcgctc agcgcggtgt
     4441 tacggaaaaa tggcagggcc atggtgtagc actgcgccag gccgctccag gtgtgggcat
     4501 acatgctgct ccacagccag acagcgaagt tggtgacaat aaagaaaaac agggacgaag
     4561 acagtgtcag tgaaattccg cgtaaaaagc ccggtttcct taaccagtag ctgctggcca
     4621 gggcaatcag cacgtaacag ccccaagtaa ccggcatgat gttgtaaaag ccgataacca
     4681 ggtcggagag cgccatcgcc acaaaaggta cccagaccgc cagccggcga ggcagcaccg
     4741 ccccgccgaa aatagctacc gccgccaccg gtgcgaagtt ggccggatgg ggcaacagcc
     4801 tgaggctgac tgccagcagt atcaggaaaa cggctatagc gaagttcagg ttggactttt
     4861 ttatcattgc agtttccaat ccagtatatc c
//
LOCUS       DASPTF010000074         3360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1127571, whole genome shotgun sequence.
ACCESSION   DASPTF010000074 DASPTF010000000
VERSION     DASPTF010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127571"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VEH48_02515"
     CDS             <1..223
                     /locus_tag="VEH48_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18265.1"
                     /translation="KFGWLELFKAVDPESVKDLWTPTPKVVSDVIAECFELNIATARV
                     LLSELPPSLREKPSFACLNDFLQQPELQP"
     gene            complement(264..557)
                     /locus_tag="VEH48_02520"
     CDS             complement(264..557)
                     /locus_tag="VEH48_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18266.1"
                     /translation="MTSVIIVGLLAGILFSFGLPHLVKGMLGKKFMTPLGQNRSAVEN
                     VMWGWAFWVVAVLLWHTAPMAAHPRATFVSAAVGVLAGGLVMAMMNPGPGAKK"
     gene            complement(634..1170)
                     /locus_tag="VEH48_02525"
     CDS             complement(634..1170)
                     /locus_tag="VEH48_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18267.1"
                     /translation="MKSKNQTGFTPVEWVLLLVIVGILAGTGLYVYRANSNTNDTLNL
                     AAGTQVSTKGATAAPSAALKKNTSDAITSGNTAALEGYMAGSVNVVIAGSEKTGAETA
                     AKAVSDLSYLNSGTAPWNFSLSTATLNSYAKGSYKQYFGANTIVGQSANGYTVSFGVN
                     GNGKIDTVFMSASTDQLQ"
     gene            1253..>3360
                     /locus_tag="VEH48_02530"
     CDS             1253..>3360
                     /locus_tag="VEH48_02530"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXY18268.1"
                     /translation="MFAKIARFSVRFRWPIIIFWIAVVPIVSSAFPNISDVTQNSTQD
                     FLPKNSPTDTAAKLETAFQKKNTASSAVLVASRGGGKLTGQDAAALQKLVDNVKKTKE
                     ITEVDDLGVSADGQAHEYLVGVSGAAFGNGAFKIVDDLREAIHNTKMPTGLKANLTGD
                     LAASVDQENANNSGRNKTEIYSVVLILVLLLLVFRSVLAPIVTLVPAGLALAISQPVI
                     AESTKAGVKVGFITQIMLIVLLLGAGTDYGLFLVFRVREEMRSKGLNAKQAVVESLTH
                     VGESITFSALTVIAALLCLMLAVFGLYSGLGPALAIGLTIMLLIALTFLPALLSVLGR
                     AVFWPTKTKQREVKIGLWGRLADRAIERPVVVLMLGVIVFGGLGSGLVGYKSAGFGTA
                     SAPAGSDSAAGQQVLERHFPAANNNPQLVIFRFKSSIWNNPQPIVKAQAELSASSVFK
                     AISGPLNANGFNLTVQQLQQIHDSGSPAESALSQFISKDGKTVQFYALLSAGNTGSAE
                     AEQAVPVVRAAVQKVAGDIGAEQNSLYSEDSVAYDINQTTNNDLKRIIPVVLLVIAAL
                     LAILLRSLVAPWYLIATVGLGYLASLGLSMYVFVHILGHGGLNFVLPFLMFVFGMALG
                     QDYNILVMSRIREEAHHSRSLFAAITKAVGITGTTVTSAGLILAGTFSIFGIVGGGSE
                     QGEQIGFGVAFAILLDTFFVR"
BASE COUNT          766 a          895 c          888 g          811 t
ORIGIN      
        1 caagtttggc tggttagagt tatttaaagc tgtagacccg gaaagtgtta aagacttatg
       61 gacacctact cccaaagtag tctcggacgt gattgcggag tgcttcgagc tgaatatagc
      121 tactgcgaga gtactactca gcgaactccc gcctagtctc cgcgagaaac caagctttgc
      181 gtgcctaaac gattttcttc agcagccaga gctccagccc tgactccaac tgcataatcg
      241 gccttgacca ctaaagaata ggcttatttt ttggcgccgg ggccaggatt catcatggcc
      301 atgaccagcc cgcctgctag cacgccgacg gctgccgaga cgaacgtcgc gcgcgggtgc
      361 gcggccatgg gcgcggtgtg ccacagcagt acggccacga cccaaaaggc ccagccccac
      421 atgacgtttt ctaccgccga gcggttctgg cccagcggcg tcatgaattt tttcccaagc
      481 attcccttca ccaaatgcgg caaaccgaaa ctaaacagta ttccggccag caatcccacg
      541 attattacgc tagtcatatc ccctccttaa ttaataattc aagcgtatca ccacttatgg
      601 ttattgcaaa ccgagagcac ttttaacagg ggtttattgc agttggtcgg tgctggcgga
      661 catgaacacg gtgtcaatct tgccgttgcc attgacgccg aaggataccg tgtagccgtt
      721 ggccgactgg ccgacaatgg tgttggcacc aaaatactgc ttgtaagacc ctttggcgta
      781 agagttaagt gtggccgtgc tcaaagaaaa gttccacggc gcggtgccgg aatttaggta
      841 actcaggtcg ctgaccgcct tagcggcggt ttcggcgccg gttttttccg agccggcaat
      901 cactacgtta accgaaccag ccatataacc ttccaaagcc gccgtattac cggaagtaat
      961 ggcgtctgag gtgtttttct taagagccgc cgaaggagcc gcggtggcgc ctttggttga
     1021 tacctgcgta ccggccgcca aattcaatgt gtcattggtg ttggaattgg ctcggtaaac
     1081 ataaagaccg gtgccggcca aaatgcccac gatcactaaa agaagaaccc actctactgg
     1141 tgtaaagccc gtttgatttt tagatttcat atcccaccct ttaattatgc tataagcata
     1201 accttaaatc gggtcgcagg caactgccag cctctggtaa aataaaagta agatgtttgc
     1261 taagattgcc cgcttttcgg tcaggttccg ctggcccatt attatctttt ggattgccgt
     1321 ggtgccgatt gtatcgtcag cctttccgaa catcagtgat gttacccaaa acagcaccca
     1381 ggattttttg cccaaaaaca gccccacgga cacggccgcc aagctggaaa cagccttcca
     1441 aaagaaaaat accgccagca gcgcggtgct tgtcgccagc cgcggcggcg gcaagctgac
     1501 cggacaagac gccgccgccc tgcaaaagtt agttgataat gtcaaaaaga ccaaagaaat
     1561 aaccgaggtc gatgatttgg gggtgagcgc tgacggccag gcccacgagt acctggtggg
     1621 tgtatctggt gcggcctttg gcaacggggc ttttaaaatc gttgatgacc tgcgtgaggc
     1681 gattcataac accaaaatgc ctaccgggct aaaagctaat ttaaccggtg atttggcggc
     1741 cagcgttgac caggagaacg ccaacaattc cggtcgcaac aaaaccgaga tctattccgt
     1801 agttttgatt ctggtcttgt tattgctggt tttcaggtcg gtattggcgc cgattgtcac
     1861 attggttccg gccggactgg cgctggctat atcccagccg gtaattgctg aatctaccaa
     1921 agccggtgtc aaagtcggct ttattacgca aatcatgctg atagttctgc ttttgggcgc
     1981 gggtacggac tacggtttat tccttgtatt tcgggtccgg gaagagatga gaagcaaagg
     2041 cctgaacgcc aagcaagcgg tcgttgaatc cctgactcat gtcggcgagt ccataacctt
     2101 ttccgccctt accgtcatag cggcgttgct gtgcctgatg ctggcggtct ttgggcttta
     2161 tagcggctta ggtccggcgc tggctatcgg cctgacaatc atgctgctta ttgccctgac
     2221 ttttttacca gccttgctat cggtattggg ccgggcggtc ttttggccga ccaagactaa
     2281 acagcgtgaa gtaaaaatcg gattgtgggg acggctggcg gacagggcga ttgaacggcc
     2341 ggtagtggtt cttatgcttg gcgtgattgt ctttggcggg ctgggatcgg gcttggtcgg
     2401 ctataaatcc gctggctttg gcacagccag cgcgccggcc ggcagcgact cagccgccgg
     2461 ccagcaagtc cttgagcggc attttccggc ggccaataac aacccgcagt tggttatctt
     2521 ccgatttaaa tcttccatat ggaataaccc ccagccgata gtcaaagccc aagcggaatt
     2581 atccgcttcc agcgtcttca aagcgattag cggaccactt aacgccaacg gctttaatct
     2641 tacggtacag caacttcaac agatacacga cagcggctca cccgccgaat cggccctgag
     2701 ccaatttatc agcaaggacg gcaagacagt gcagttctac gccttactta gtgctggcaa
     2761 caccggcagt gccgaagccg aacaggctgt gccagtagtc cgtgccgcgg tgcaaaaggt
     2821 cgcaggagac attggcgccg agcaaaacag cctttacagc gaagactcgg tcgcctacga
     2881 catcaaccag actaccaata atgacttaaa gcggatcatt cccgtagtgc tgctggtgat
     2941 cgccgctttg ctggcgatac tgctgcgcag cctggtcgcg ccgtggtatt taatagcgac
     3001 cgtcggcctg ggttacttgg cctcgcttgg gctatcaatg tacgttttcg tgcatatact
     3061 cggccatggc ggcttaaact tcgtcctgcc attcttgatg ttcgtgttcg gtatggccct
     3121 cggacaagac tataacattc tggttatgag ccgtatccgc gaggaagccc atcatagcag
     3181 atcactgttc gccgctatca ccaaagctgt tggcattacc ggcacgactg tcacatcagc
     3241 cggtttgatt ttggccggga cattttcaat ctttgggatc gttggcggcg gcagcgaaca
     3301 aggcgaacaa attggctttg gcgtggcttt tgccatcctg cttgatactt tctttgtgcg
//
LOCUS       DASPTF010000075         5836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1129010, whole genome shotgun sequence.
ACCESSION   DASPTF010000075 DASPTF010000000
VERSION     DASPTF010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129010"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VEH48_02535"
     CDS             <1..1054
                     /locus_tag="VEH48_02535"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18269.1"
                     /translation="EAKLLAPELIMVETDPPKYHYVYKKLIKIMRSYSPNIGMKSIDE
                     GVIDFHGTREVINTRPLTVIGQEIKDRLRAEVGSWMKCNIGIAPNRFLAKTAASLHKP
                     DGLDVITHKNLHHTLATMQLTDLTGIAERNQARLNAAGIYTPLQFLDAPSELLVRKVF
                     CSICGEDWHKRLRGWEIDDVEYATKTVGRQFVMDDYNASDEKVRNRLAHLCESTGLKL
                     RYNGFCARGVSLHIQYKSGDAWHSRRAFKSSFYTTPEIFRRALLLFNQQPRLDRIVML
                     SVSCYLLEPSSANQLSILEEVNKERFLTEAVDELNWRYGNYTVTFANSLGASQQVKQK
                     IPFGSTRYFELLCNRT"
     gene            1145..2395
                     /locus_tag="VEH48_02540"
     CDS             1145..2395
                     /locus_tag="VEH48_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXY18270.1"
                     /translation="MGLFKRKSNGSHGRKSRSGLAVSEKVLKDERQSSDFILGAIEDG
                     VLLVSRDNLIQLINPGASTISGWPAEEAVGLDFHNVLKLVNQKGVPLIAENHPFSKAL
                     ASGVAVRDSRAWLATRGGKVVPISLIVSPVLDENDRPTGNVVGVFRDITKEQAEEAQR
                     SDFVSTASHEMRTPLAAIEGYLSLAANPKIATMDNNARKYIDKAAAATKHLGVLFQDL
                     LTSSRAEDGRLASYPAVVEIGETVQQVVDDGRFKAKDKGLELHFIVSADKDVSGGRVV
                     RPLYYTYADPNRIREVLQNLIDNAIKYTTSGGIAIRLTGDQGVVQLQVSDSGPGIPPE
                     DIPHLFQKFYRVDSSMTRTVGGTGLGLFISRKIVELYNGRIWVESQLGKGSTFFINLP
                     RLTTEQALATQKRQAALVSPLDIQ"
     gene            2478..3272
                     /locus_tag="VEH48_02545"
     CDS             2478..3272
                     /locus_tag="VEH48_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXY18271.1"
                     /translation="MAKVLLVEDDQNLREIFEMRLKAEGYETVVAGDGEEALTVAVKE
                     KPDLIIADVMMPRLSGFEMLETLRAAPDMAATKVVMMTALGQAEDQARGEKLGVVKYL
                     VKSQVTLEDFVRVVREVLQPDQKPSQQPDSSINNKKESENSMPDDSNAPTNPAPDAPA
                     GPMPDANPISGAPAMPDAPADSGQMSTAQEQSAVNNQMSDFASSAPVTPPADTGAPAA
                     EPDEPAAPADTGSVPVSPPPAADEPAAPAGGDSGTPPAPADSGSGL"
     gene            3351..4685
                     /gene="der"
                     /locus_tag="VEH48_02550"
     CDS             3351..4685
                     /gene="der"
                     /locus_tag="VEH48_02550"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HXY18272.1"
                     /translation="MSKRIPIVAIVGRANVGKSSLFNTLIGRREAIVAKEAGTTRDNV
                     TAMASYGGKDFWLVDTAGLKAAEDDFELGIQQQIIEAAASADVIIVVVEADVPPTAAD
                     RQGATMALKSLKPVVLVINKIDKNKNVSLSDWQRLGIKTVVATSVSQKTGLEELLDAI
                     SDRLPKASIKTAKDRLRLALLGRPNVGKSSLFNTLATKQQALVHSSAGTTRDVNRAGV
                     RFMGKEIEIMDTAGIRRSGKIARGVEQFSVLRALAAIEEADICLVLMDVGELNVQLDQ
                     KIAGLVVDAGKGLILVVSKWDSLEKDSFSRDVLAAQIADTFVFVPWAPLVFTSAVTGQ
                     NVAKIFELALRIEENRSIKFTTPELNKWLRAAVDQHPPAGLKNRQPKLNYIVQEDRTG
                     LPSFKIFGAHTRFLHWSYKRYLEKSFRKQWPLTGTPLKFWFIEKEGRRVRGS"
     gene            4746..5381
                     /gene="pth"
                     /locus_tag="VEH48_02555"
     CDS             4746..5381
                     /gene="pth"
                     /locus_tag="VEH48_02555"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXY18273.1"
                     /translation="MALFQKKPQISSSVPLYTIGSNKTVLIIGLGNPGKEYEGTRHNV
                     GFAILDEFAAKNDFPAWIAKKDLRSHITSANLGENRVVLCKPAIFVNNSGEAAQAVQH
                     FYRVYNQQTLVVYDELAIPFGGLRTRLGGTDAGHNGVKSLIAHIGDDFGRLRIGIGSE
                     TAQKADAADFVLSKFSKKEQGNLPLIIREANALITEYVFGGQLPHDTRNVL"
BASE COUNT         1499 a         1596 c         1602 g         1139 t
ORIGIN      
        1 cgaggctaag ttgttggccc cggagctaat catggttgag accgacccgc ccaagtacca
       61 ctacgtctac aaaaaactga ttaaaatcat gaggtcttac tcgcccaata tcggcatgaa
      121 gagcattgac gagggtgtga ttgattttca cggcacgcgc gaggtaataa acacccggcc
      181 gctgacggtc atcggccagg aaatcaagga ccgcctgcgg gctgaagtcg gtagctggat
      241 gaagtgcaat atcggcatcg ctcccaaccg ctttttggcc aagaccgctg cctccttgca
      301 caaaccggac ggcctggacg ttatcaccca taaaaatctg caccatacgc tagccactat
      361 gcagttaaca gatctgaccg gcatcgccga gcgcaaccag gcacggctga acgccgccgg
      421 catttatacg ccgctgcagt ttctggatgc gcccagcgag ctgctggtgc gcaaagtttt
      481 ttgcagcatt tgcggcgaag actggcataa gcggctgcgc ggctgggaaa tagacgatgt
      541 ggaatacgct accaaaaccg tcggccggca gttcgtgatg gacgattaca acgcctccga
      601 tgaaaaagtt cgcaaccgct tggcgcacct ctgcgaaagc accggcttaa agctgcgcta
      661 caacggtttt tgcgcccgcg gcgtcagtct tcacatccag tacaaaagcg gcgatgcctg
      721 gcacagccgc agggccttta aaagcagctt ttataccacg ccggagattt ttcgccgggc
      781 gctgttgcta ttcaaccagc agccgcggct ggaccgcata gtgatgctta gcgtcagctg
      841 ctatttgctg gaaccgtcca gcgctaacca gctgagcatt ttggaagagg tcaataaaga
      901 aaggttttta actgaggccg tggatgagct gaactggcgc tatggcaatt acaccgtaac
      961 ttttgccaac agcctgggcg ccagccaaca ggtcaaacaa aaaatcccgt tcggctcgac
     1021 acgctacttt gaactcctat gcaacaggac ttagttgaaa gataggctaa aataacagat
     1081 aatgtgaagc gttagcgaaa tagcctaaaa cagatgaccg acgaaagcca ggtgggaatt
     1141 atcagtggga ttatttaaac gcaagtccaa cggctcgcac ggccgcaaaa gccgcagcgg
     1201 cctggcggtc agcgaaaagg tcttaaaaga cgagcgccaa agttcggact tcatactggg
     1261 cgccatagag gacggcgtac tactggtcag ccgcgacaac ctgatacaac tgattaatcc
     1321 cggagccagc acgatcagcg gctggccggc tgaagaggcc gttggcctgg attttcacaa
     1381 cgtgctgaag ctagtcaatc aaaagggagt gccgctgata gcggagaacc acccctttag
     1441 caaagcccta gccagcggcg ttgcggtgcg cgacagccgg gcttggctgg ctaccagagg
     1501 cggtaaggtc gtaccgattt ccctgatcgt ttcaccggtg ctggacgaga atgaccgccc
     1561 aaccggcaac gtcgtcggcg tttttcgcga tatcaccaaa gagcaggccg aagaagccca
     1621 acgctccgac tttgtcagca ccgccagcca tgagatgcgt acaccgctgg cagccataga
     1681 gggctacctg tcgctggccg ccaaccccaa gatcgccact atggacaaca atgcccgtaa
     1741 gtacattgat aaggccgcgg ctgctaccaa gcacctgggc gtactgttcc aggaccttct
     1801 gaccagttcg cgcgcggaag atggccggct ggccagctac ccggcagtgg tggaaatcgg
     1861 cgaaacagtg cagcaggtgg tggatgatgg ccggtttaaa gctaaagaca aaggactgga
     1921 gctgcatttc atagtcagcg ctgataaaga cgtcagcggc ggccgggtgg ttaggccgct
     1981 atactacact tacgccgacc ccaatcgcat ccgtgaagtt ttgcagaacc ttatagataa
     2041 cgccatcaaa tatacgacca gcggcggtat cgccatccgc ctgaccggcg accagggtgt
     2101 agtgcagctg caggtctccg acagcggccc gggcatcccg cctgaagaca taccgcacct
     2161 gttccaaaaa ttctaccggg tggacagctc gatgaccaga acagtcggcg gtaccggctt
     2221 gggcctattc atcagtcgta aaatcgtcga gctctataac ggccgtatct gggtggaaag
     2281 ccagctgggc aaaggcagca ccttttttat caacttaccg aggctgacga ctgaacaggc
     2341 cctagcgacg cagaaacgcc aggcagcttt ggtcagtcca ctggatatac agtaggggtt
     2401 acaaaagtga attcatgcta caataaactc aaacataagc gttaacagat gagcaagaag
     2461 ggatagacaa agcgggcatg gccaaggtat tgctggtaga ggacgatcag aacctgcgcg
     2521 aaattttcga gatgcgcctg aaagccgagg gctacgagac ggtggtcgcc ggtgacggtg
     2581 aagaagccct aacggtcgcc gtcaaagaga agccggatct gattatcgcc gacgtcatga
     2641 tgcctagatt gtccggcttt gagatgctgg agaccctgcg cgcggccccg gatatggccg
     2701 ccaccaaagt ggttatgatg accgccctgg ggcaggccga agatcaggct cgcggagaaa
     2761 agctgggcgt ggtaaagtat ttagtgaagt cccaggtcac cctggaagat ttcgtccgtg
     2821 tggtacgcga ggtcttgcag ccggaccaaa aaccaagcca gcagccagac tcctcaataa
     2881 ataataagaa agaaagcgag aattccatgc cagacgattc taacgcacca actaatccgg
     2941 cgcccgatgc tccggccggc ccgatgccag acgccaaccc tatcagcggc gcgccagcca
     3001 tgcctgatgc tcctgccgac agcggccaaa tgagcaccgc tcaggagcaa agcgccgtta
     3061 acaaccaaat gagcgatttc gcgtccagcg caccagttac accgccggca gataccggcg
     3121 caccggcagc tgaacccgac gaaccagccg cgccagccga caccggcagc gttccggtat
     3181 caccaccgcc cgcggcggac gaaccggccg cgccagccgg cggcgacagc ggcactccgc
     3241 ccgctcccgc cgacagcggc agcggtctgt aaagacaaag acccagaaaa actgcgccca
     3301 ggtgtttgac aggtgcgcag ttttttaatt ttacaagtat aataacacct atgagtaagc
     3361 gtattccaat cgtggctatt gttgggcggg ccaacgtcgg caaatccagc ttgtttaaca
     3421 cgctcatcgg ccggcgtgaa gcgattgtcg ccaaagaagc cggcactacc cgcgataacg
     3481 ttaccgccat ggccagttac ggcggtaagg acttctggct ggtagacacc gccggcctga
     3541 aagcggccga ggacgatttt gagctgggta tccagcagca aatcattgaa gcggcggcca
     3601 gtgccgacgt cataatagtg gtggttgaag ccgacgtgcc gccgaccgcc gccgaccgcc
     3661 aaggcgccac tatggctctg aaaagcctca aaccggtagt gctggtcatc aacaaaattg
     3721 ataagaataa gaatgtttcg ctgagcgact ggcagaggct gggcataaaa accgttgtcg
     3781 ccaccagtgt cagtcaaaag accggcttgg aagagttgct ggatgccata agcgaccggc
     3841 ttcccaaagc cagcatcaag acagctaaag accgcttaag gctggctctg ctgggccggc
     3901 cgaatgtcgg caaatcttcg ctttttaaca ctttagccac caagcagcag gcgctggtgc
     3961 actccagcgc cggcaccacc cgcgatgtca accgggccgg cgtgcgtttt atgggcaaag
     4021 agatagagat catggatacg gccggtatcc gccgcagcgg caaaatcgcc cgcggcgtgg
     4081 agcagttcag cgttctgcgg gcgctggcgg ccatagagga agctgatatc tgtctggttt
     4141 tgatggacgt tggcgagctg aacgtccagc tggaccagaa aatcgccggc ctggtggttg
     4201 atgccggcaa gggattgatc cttgtggtca gcaaatggga cagcctggaa aaagacagtt
     4261 tttcgcgcga cgttttggct gcccagatag ccgatacttt cgtttttgtt ccgtgggcgc
     4321 cgctggtgtt caccagcgcc gtgaccggcc aaaacgtcgc taaaatcttt gagttagctc
     4381 ttcggataga agagaaccgc tctataaaat ttacaacacc cgagcttaat aaatggctgc
     4441 gcgccgccgt tgaccagcat cctccggccg ggctcaaaaa ccgccagccc aaacttaact
     4501 acatagttca ggaagacagg acaggtttgc caagcttcaa aatcttcggc gcccatacca
     4561 gatttctgca ctggagctat aaacgctacc tggagaagtc atttcgcaaa caatggccac
     4621 tgaccggcac accgttaaag ttctggttta tcgaaaaaga gggtcgtagg gtcagagggt
     4681 cataggtata caggtgcttc tacaacccta taaccctaaa accctattac ccttataata
     4741 cttttatggc gctttttcag aaaaaacccc agatcagcag cagcgttccg ctttatacca
     4801 ttggctcaaa caagactgta ttgatcattg gactaggcaa tccgggcaaa gaatatgagg
     4861 gtacaaggca taacgtcggt tttgcaatac tggacgaatt cgctgccaag aatgattttc
     4921 cggcctggat cgctaagaag gacttgagga gccatattac gtcagccaac ctgggcgaga
     4981 accgggtggt tttgtgcaaa ccggcgattt ttgttaacaa cagcggtgag gccgcccagg
     5041 cggtgcagca cttttaccgg gtctataacc agcaaacctt ggtggtatac gatgaactgg
     5101 ccatcccatt cggcggtttg cgcacccgcc tgggcggcac ggacgccggc cacaacggcg
     5161 taaaatcact tattgcccac attggcgatg attttggccg cctgcgcatc ggcatcggct
     5221 ccgaaacggc ccaaaaggcc gacgccgccg atttcgtgtt gtctaaattc agtaaaaaag
     5281 agcaggggaa tctcccactt attatccgag aagccaacgc tctgataact gaatacgtct
     5341 tcggcggcca gctgccccac gacacccgca acgtgcttta aaaacgcaaa gagctccagc
     5401 gggggtgggc accgttggag ctctttgcgg gctcgagacc tgctcaagcc caaaaaacac
     5461 ccttcaaccc accttgagcg aagctgcgaa gacggctaaa taagcaacgt gcgttcgcgc
     5521 cggccgccag aggcggccat ctctcgtgag agagactcgc cgacccactt gagtcgggca
     5581 agagttaaag gggttagttt gtttaagaac ccagctctga cgtatactat caagtagtaa
     5641 aacgtttagg gctgtaaggc tcagaagtgg agggtaacaa cttccgaggc caaaaagctc
     5701 ttaaacaatc taacaaatgt tagaaattga tatctaagac tctagagtcc aagataccta
     5761 ttgggggtat aaggtactta aaaatccgct gaagcggaac ttacgagttg caattttagc
     5821 aaataaatgg cttggt
//
LOCUS       DASPTF010000076        10072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_557736, whole genome shotgun sequence.
ACCESSION   DASPTF010000076 DASPTF010000000
VERSION     DASPTF010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10072)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10072
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557736"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VEH48_02560"
     CDS             complement(<1..616)
                     /locus_tag="VEH48_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXY18274.1"
                     /translation="MSEDKKPQKSQFALIEEGLVELWQKDKTFEKSVARREGEERFNF
                     NDGPPFANGLPHFGHSLVTGIKDSILRYKTMRGLYVPRRNGWDCHGLPVEYAIEKQFG
                     ISGKKQILELGLEKFNTACRDSIFTYKADWEAFLNRYGRWSDYEHSYATVDRDYTESV
                     WWILSQINQKGLLYRGLKSVPYCPRCATPLSNFELNEGYRDNVPD"
     gene            complement(790..1104)
                     /locus_tag="VEH48_02565"
     CDS             complement(790..1104)
                     /locus_tag="VEH48_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18275.1"
                     /translation="MRVVALYHPQSDHGGLVTDYAAEFEHYKGKTLDLISLESTEGAD
                     LAELYAVARYPAILVMSDDGSLQRLWQGTPLPLMDELSYYAQPPQQPISRFGRRVVAL
                     PA"
     gene            1181..1843
                     /locus_tag="VEH48_02570"
     CDS             1181..1843
                     /locus_tag="VEH48_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18276.1"
                     /translation="MVQTVESQSEKQSEDQSLLLRQLLLPSAGQVALDLLVAILLLLL
                     FNIRPLWHYLNFTVLGLNQSDSGGVFAQAPGWLQAPLKAATTGRLPQIAFWAFAGTGL
                     YLVAWFAKNAVINIYNDIVASRFTRPLTYRAEQFWSSVLMQKLFFGGGLAVLAGYGYM
                     LVRLLGVLSQTCHSALANFSLLDGSLKVTTSIIGLAVLLRVFMILLHVVIRSWSNINQ
                     GL"
     gene            complement(1879..4029)
                     /locus_tag="VEH48_02575"
     CDS             complement(1879..4029)
                     /locus_tag="VEH48_02575"
                     /inference="COORDINATES: protein motif:HMM:NF028151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HXY18277.1"
                     /translation="MAKKHMPPPETKQNTTDSKSSKKGLLDPSRFSRNQLITIAVIFA
                     AIGGYVLYRSFASVYLVTSLQAEQMTLPASQWDYAYADSTASGGKAVALLSGGTLTGT
                     VSFPSSVTSLTVVAKGDQCNGAPTMIVKVDGTTILNSQAVTSTSWADYSANVSLATGS
                     HTLSIRFNNDYYNNSCDRNLYVDVTNFYGPVVSTASLVTSLQAENMTLPPSKYASIYS
                     DSTASGGKAVALDEGGSLGTLTGTVSFPSSVTSLTVVAKGDQCSGAPTMNVVLDSTTV
                     MNQLPVSATGWTSYSATLANAASSGSHSLSIAFTNDYNNSSCDRNLYVDVTNFYGSGI
                     VPASPAPTVSISPSPASVTAGSASTLTWSSANASSCTASGAWSGSQPTSGSSSTGALN
                     QNSTYTLTCTGSGGSASASATISVSAAASGGGGGSASGPTGGVWSWNAATATLDPNSA
                     SKISAFQSYAVGAGVYFQAHTAWINAPAGTPAYSIPMVESFPAVTVPIPLGTKPGNTN
                     DGELTVLDLSTGINYDFGNATYNSTSGKITGAFGIGAVQPGSLWESPGSASAAAMSVR
                     PLLIFPSDIASGVIAHPLAFSIPAGPGGGVTATPVYPARSGVSPEPGSGLPLGTWLRL
                     DPTINVPSLGLNAFETMILVALQKYGMFNRDAGSTLTIYGTDQVNQGGNAADWGAVGV
                     SLPLTNNGIGYASQFSSGFVAQMAHLQVLLPPSH"
     gene            4128..4316
                     /locus_tag="VEH48_02580"
     CDS             4128..4316
                     /locus_tag="VEH48_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18278.1"
                     /translation="MERFPEILVGVAVVSGVVYLLEQKSQTGIDYAVKWTSIILMIVS
                     LLGAIAIFLIDQKRKTRP"
     gene            complement(4524..7412)
                     /locus_tag="VEH48_02585"
     CDS             complement(4524..7412)
                     /locus_tag="VEH48_02585"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HXY18279.1"
                     /translation="MAQATKLTVRRLFTKPDSKAYEQLKWHKQDSLITNPATGKAVFE
                     QKGVEFPEGWSLNAVNIVAQKYFSGTPGTKTRESSLKHLVDRVADTITRAGLQEGYFD
                     SQAEAEDYREELKYILATQRAAFNSPVWFNIGVLERAQQASACFILGVEDTMESILNW
                     YTEEGMIFKGGSGSGINLSSLRSSKEKLGDSAGRSSGPVSFMRGADSSAGSIKSGGKT
                     RRAAKMVILNVGHPDVEEFIWCKAIEERKARVLSEAGYDMDLDGRDSFSVQYQNANNS
                     VRVTDDFMRAVENDGDWELRAVTTGQAIETVKARDVWRQIAEAAWECADPGLQFDTTI
                     NRWHTAPLAGRINASNPCSEYMHLDNSACNLASINLLKYLRDDGSFDVESFKHTVEII
                     FTGQEILVGYSQYPTKSIAKNARAYRELGLGYANLGALLMAQGLPYDSEEGRAQAAAV
                     TALMTGHAYATSAKLARKVGPFAGYSQDRAAMNKVLKMHRDEVKKIDAGLVSEELLSA
                     AASAWDEAVELAELHGVRNSQASVLAPTGTIAFFMDCDTTGIEPDLGLVKFKKLVGGG
                     SMTIVNQTVPRALKSLGYKPAQIEDIVSYIDSEKTIVGAPHLKDEHLPVFACSMGDNA
                     IHYLGHVKMMGAVQPFISGAISKTINMPEDVTVEDVEQIHMDSWKLGLKAVAIYRDNC
                     KVAQPLSMAKKEGTETPAVAATVPAAQEQPVAVGGETVVAAGSAPKNAVRHELRRIRN
                     AKNFKFTVAGTRGYFIVGEYDDGSPGELFVSIAKQGSTLRGVMDAFAVAVSHGLQYGV
                     PLKKFIRSFSHTSFAPAGITDDPELRTASSLIDYIFRRLGKTYLSFDEQLEVGLANID
                     DIPPGQTSLLAPAEAEGQEASKEQMEQAIEAVAEAEAAITEAVTTIAASTAPTPQITA
                     QAETPASAPKPLPVQDPSAPICDNCGNQTVRAGSCYVCTSCGSTTGCS"
     gene            complement(7580..8071)
                     /gene="nrdR"
                     /locus_tag="VEH48_02590"
     CDS             complement(7580..8071)
                     /gene="nrdR"
                     /locus_tag="VEH48_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXY18280.1"
                     /translation="MKCSHCGSTDNKVIESRDVADGEAVRRRRQCLKCGNRFTTYERL
                     ERPMLVVIKRDGSRQLFSREKLMAGLQHATEKTAVTHMQLEAFVADLEHELYSRGEPE
                     IASVDIGELVMERLVNLNEVAYVRFASVYRKFRDISSFERELSKIRKSKSAGKSSAKK
                     PSK"
     gene            complement(8211..9611)
                     /gene="ftsZ"
                     /locus_tag="VEH48_02595"
     CDS             complement(8211..9611)
                     /gene="ftsZ"
                     /locus_tag="VEH48_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXY18281.1"
                     /translation="MPKVDPAIETFARIKVVGVGGAGGAAINRMVDAGVTGVDFVAIN
                     TDAQALHHNRAGNKVHIGKDATKGLGAGADPVKGQQAAEESRPEIEQAIKGADMVFVT
                     IGAGGGTGSGAGHIVAELAKDSGALVVGFATKPFAFEGEKRRQNAEVAIDKMRKNVDT
                     MITIPNDKLLTTIDRRTPLLEAFKVADDVLRQGVQGISDLITVHGLINLDFADVKAVM
                     ADAGSALMGIGRASGENRAIEAATQAISSPLLEVAIDGARGILFNVIGGNDMSMHEIN
                     TVAETITAAADSEANVIFGATINPDLEGEIIVTVVATGFDDSYYASRDAKAAELLRRH
                     TQESLARTSQVDDEAMKDLDMELKEPVETTAEDFHSDDTPNIWALPSDDVNGVPQDEE
                     EEEDELDKPSFLRRLTGRAKKDDNERQAAKAPADDSADEDDQDADDKKPEDDKDQPDE
                     AEPDKSDDAKSKKSKN"
     gene            complement(9744..>10072)
                     /locus_tag="VEH48_02600"
     CDS             complement(9744..>10072)
                     /locus_tag="VEH48_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18282.1"
                     /translation="KELKRIHRSRRLPGGVVLVGGTAKLPGVAEFARDVLELPARVGK
                     WGHINKVVDGLDEERFAPCVGLMLLDMLLGPPAGGGHFNEAEPGLLESVNESFSGLLR
                     RLKRRK"
BASE COUNT         1967 a         2769 c         2938 g         2398 t
ORIGIN      
        1 ggtcggggac gttatctcgg tagccctcgt tgagctcaaa gttagacagc ggcgtggcac
       61 agcgcgggca gtacggcacc gacttcaaac cccgataaag caagcctttt tggttgattt
      121 ggctcaaaat ccaccaaacg ctctcggtgt agtccctgtc tactgtggcg tagctgtgct
      181 cataatcgct ccagcggccg taacggttta agaaagcctc ccaatcggct ttataagtga
      241 agatggaatc gcggcaagcg gtgttgaact tttccagacc aagctccaga atctgttttt
      301 tgccggatat accgaactgt ttttcgatgg cgtactccac cggcaggccg tggcagtccc
      361 agccgttgcg gcgcggcaca tacagcccgc gcatggtttt ataccttaaa attgagtctt
      421 tgatgccggt gaccagcgag tggccgaaat gcggcaggcc gttggcgaac ggcggtccgt
      481 cgttgaaatt gaagcgctcc tcgccctcgc gccgggcgac acttttttca aaggttttat
      541 ctttttgcca aagctcaacc aggccctctt caatcagggc aaactgcgat ttttgcggct
      601 ttttatcctc actcattact aaaactatac cccagatagc acccgagagc caccattgaa
      661 ttgacctaat tgacctaatt tctaattgat ctaagcaatg actaagtacc aattatccaa
      721 acagcttggg tcgtttggta tttaggactt atttagtaat tagagcaatt ataaataatt
      781 aggaattttt taagctggaa gagcgactac tctgcgcccg aaacggctga taggctgctg
      841 gggcggttgg gcgtaataag acagttcgtc catcagcggc agtggtgtgc cctgccatag
      901 acgctgcagg ctgccgtcgt cgctcatcac caggatggcg gggtagcgcg caacggcgta
      961 aagctcggcc agatcggcgc cttcggttga ttccaggctg atcaagtcca gcgttttgcc
     1021 tttgtagtgt tcaaattcag cggcgtaatc ggtaaccaaa ccgccgtggt cgctttgagg
     1081 atgatataaa gccacgacac gcatataaat ccatactagc tcaagtatgg cgatgacgcc
     1141 aggcaaattt agtataaaat agtcatgagc gtctaaaaat atggtccaaa ccgtagaatc
     1201 tcagagcgaa aagcagagcg aagaccagtc actgctgttg cggcagttgc tgctgccgtc
     1261 ggccggccag gtggcgctgg acctgctggt ggcgattctg ctgcttctgt tattcaatat
     1321 ccgcccgctg tggcactatt taaactttac ggttttaggg ttgaaccaga gcgatagcgg
     1381 cggcgttttt gcccaggcgc cgggctggct gcaggcgccg ctgaaggccg caaccaccgg
     1441 ccggctgccg caaatcgcct tttgggcttt tgccggtacc ggcctttatc tggtcgcctg
     1501 gttcgccaaa aacgctgtta tcaacatcta taatgatatc gttgccagcc ggtttacccg
     1561 gccgctgacg taccgcgccg agcagttttg gtcgtcggtt ttaatgcaaa agttgttttt
     1621 tggcggcggt ctggcagttt tagcaggtta cggctatatg ctggtgcggc tgttgggcgt
     1681 tttgagccag acttgccatt cggcgctggc caactttagc ctgttggacg gcagtctgaa
     1741 agtgactacc tcaattatcg ggctggccgt actgctacgg gtatttatga ttctgctgca
     1801 cgtcgttatc cggtcctggt caaatatcaa ccagggtcta taagaagcta ccgtacttct
     1861 ttctttagag ttaaaagtct agtggctagg ggggagcaga acttgtaagt gggccatctg
     1921 agcgacgaag cccgaggaga actggctggc gtagccaatg ccgttgttcg taagcggcaa
     1981 gctaacacca acagcgcccc agtcggcggc gttgccgcct tggttcacct ggtcggtacc
     2041 gtagatggtc aaggtgctgc cggcatcacg gttaaacatg ccatactttt ggagagcaac
     2101 aagaatcatc gtctcgaagg catttagacc aagacttggc acgttgatgg ttggatcgag
     2161 acgcagccag gtgccaagcg gcagaccgct gccgggttcg ggacttacac cggaccgggc
     2221 cggatacaca ggtgtcgcag ttacccctcc gccagggccg gctggaatgg aaaatgctag
     2281 aggatgggca attacgcctg aggcgatatc ggaggggaag atgagcagag gccggacgct
     2341 catcgccgcc gcgctggcac ttcctggtga ctcccataat gaacctggct gaacggcccc
     2401 tattccgaag gcgcctgtga tttttccgct tgttgagttg taggtagcgt tgccgaagtc
     2461 gtagttaatt ccggtcgaga gatcaagcac tgttagttca ccgtcgttgg tgttacccgg
     2521 tttcgtccca agtggaatgg gtaccgtaac cgccgggaaa ctttcgacca tcggaattga
     2581 gtaagctgga gtgccggcgg gcgcgttaat ccaagcggtg tgcgcctgga agtagacacc
     2641 cgcccctacc gcatagcttt ggaaggcaga gatcttggag gccgagttcg ggtcaagggt
     2701 agcggtggca gcattccaag accatacgcc gccggtaggt cccgaggcgc tgccaccacc
     2761 gccaccggag gcggcggcgg aaacactgat ggtggcactg gcgctggcgc tgccgccgct
     2821 accggtgcaa gtaagtgtgt aggtactgtt ttggttcaag gcgcccgtag aactggagcc
     2881 ggaagtcggc tggctgccgc tccaggcgcc ggaagccgtg caagagctgg cattagcgct
     2941 gctccaggtc agagttgaag cactgccggc ggtaaccgac gccggtgagg gcgatattga
     3001 cacggtgggt gcgggtgatg ccggcacaat gcccgagcca taaaaattag taacatccac
     3061 ataaaggttg cggtcgcagg atgaattatt gtagtcattg gtaaaggcga tggagaggct
     3121 gtgactgccg gagcttgcgg cattggcaag agtggcgctg tagctggtcc agccggtagc
     3181 ggataccggc agctggttca taaccgttgt gctgtcaagc actacgttca tggtcggcgc
     3241 gccgctgcac tggtcgcctt tggccacgac tgtcaatgag gtaacgctgg aggggaaact
     3301 caccgtgccg gtcagagtgc caagactgcc tccctcatcc agagctacgg ccttgccgcc
     3361 ggaggcggtg ctatcgctat aaatggaagc gtacttagat gggggcaagg tcatattttc
     3421 ggcctggaga gaagttacca aggaagcggt tgatacgacg ggaccgtaaa aattagtgac
     3481 atcaacgtat aagttgcggt cgcaagagtt gttgtagtag tcgttattaa atctgatgct
     3541 aagcgtgtgg ctgccggtgg ccagcgaaac attggcgcta tagtcagccc agctggtact
     3601 ggtgactgcc tgactattaa gaatggttgt gccatcgacc ttgacgatca tggtcggcgc
     3661 gccgttgcac tggtcgcctt tggccacgac tgtcaatgag gtaacgctgg aggggaaact
     3721 caccgtgccg gtcagagtgc cgccgctcag cagagccact gccttgccgc cggaggcggt
     3781 gctatcggcg taggcatagt cccattgtga ggccggcagg gtcatttgtt cggcctggag
     3841 agaagttacc agatagacag acgcaaaact gcggtacagc acgtagccgc cgatggcggc
     3901 gaagatcaca gcgatagtta tcaattgatt gcgcgaaaag cgtgacgggt ctagtaagcc
     3961 ctttttggag cttttgctgt cagttgtgtt ctgcttggtt tctggcggtg gcatgtgttt
     4021 cttggccaag actctaccct tttaatataa ccttgagcac cattgttgag cagccgccgc
     4081 gccctgtcaa gcctatttta ctgctctaca gagtgtatac ttggcttatg gagcgatttc
     4141 cggaaatttt ggttggcgtg gcggttgtca gcggcgtggt ttacctgctg gagcaaaaat
     4201 cccagacggg tattgattat gctgtcaagt ggacttcaat catcctaatg atcgtttctc
     4261 tgctgggagc cattgccata ttccttattg accagaaacg caaaacccgc ccttagccaa
     4321 gccactaccc aagaacccca aaatacttaa catgtcaatt attccctgca tgtgtaagcg
     4381 agctccaact ttcagccagg cctgaccttg cccaagcgga gccgggccgg tcggctgtgg
     4441 agatgcgata atttactttc gcagctcaga cagccgaccg tccgcgcgtg agcggtctgg
     4501 atgaaagttt ggagcgagcg gttttaactg cagcctgtcg tgctaccgca ggaagtacac
     4561 acgtaacatg agccggcacg cacggtttgg ttgccacagt tgtcgcaaat tggcgccgaa
     4621 gggtcttgaa ccggtagcgg ttttggagct gaagctggcg tttcggcctg ggcggtaatt
     4681 tgcggcgtgg gtgcggtgct ggcggcaatc gtggtgactg cctcagtgat ggcggcctcg
     4741 gcctcggcca cggcttctat ggcttgttcc atctgttctt tggaggcttc ttggccttcg
     4801 gcctcggccg gggcaagcaa gctggtctgg ccagggggga tgtcgtctat attggccaaa
     4861 ccgacttcca gctgttcgtc aaagctcaga taggttttgc ccaggcgacg gaaaatgtag
     4921 tctattaacg agctggcggt gcgcagttcc gggtcgtcag tgatgccggc cggcgcgaag
     4981 ctggtgtggc tgaagctgcg gatgaatttt ttgagaggca cgccatactg caggccatgg
     5041 ctgacagcga cagcgaaagc gtccatgacg ccacgcaggg ttgagccctg cttagcgata
     5101 ctgacgaaca gctcgcctgg gctgccgtcg tcgtattcgc cgacgatgaa gtagccgcgg
     5161 gtgccggcga ccgtgaactt gaagttctta gcgttacgga tgcgcctaag ctcgtggcgc
     5221 acggcgttct tgggcgccga accagcggcc acgacggttt ccccaccgac cgcgaccggc
     5281 tgttcttggg cggccggtac ggttgcggcc acagccggag tttcagtacc ctctttcttg
     5341 gccatggaca gcggttgggc gaccttgcag ttgtcgcggt aaatggccac ggctttaagg
     5401 ccgagtttcc aggaatccat gtggatttgc tccacatctt caaccgtcac gtcttcgggc
     5461 atgttgatgg ttttagaaat ggcaccggaa atgaacggct ggacggctcc catcatcttg
     5521 acgtggccca ggtagtggat ggcattatcg cccatgctgc aggcaaacac cggcaggtgt
     5581 tcgtctttaa ggtgcggcgc gccgacgatg gtcttttcgc tgtctatgta gctgactatg
     5641 tcttctatct gcgccggttt gtagcccagg ctcttgagtg cccgcggcac agtctgatta
     5701 acaatggtca tggaaccgcc gccgaccagc tttttgaatt tgaccaggcc gaggtccggc
     5761 tcaatgccgg tggtgtcgca gtccataaag aaagcgatgg tgccggttgg agccaggacc
     5821 gaggcctggc tgttgcgtac gccgtgaagc tcggccagct ccaccgcctc gtcccaggcg
     5881 ctggcggcgg cagaaagcag ctcttcgctg accaggccgg cgtcaatttt cttgacctcg
     5941 tcgcggtgca ttttcaggac tttgttcatg gccgcgcggt cctggctgta gccggcgaac
     6001 ggcccgactt tacgggcaag tttggcgctg gtggcgtagg cgtggccggt cattagtgcc
     6061 gtaacggctg ccgcctgggc gcgcccctct tcggaatcgt acggcagacc ctgggccatc
     6121 agcagcgcgc ccaggttggc gtaacccaaa cccagttccc ggtaagcccg ggcgtttttg
     6181 gcgatgcttt tggtggggta ttggctgtag ccaaccagga tttcctggcc ggtaaagatg
     6241 atctccacgg tgtgtttgaa gctctcgacg tcaaagctgc catcatctct caaatacttc
     6301 agcaggttga tggaggctaa gttgcaggcc gagttgtcca ggtgcatgta ctcactgcag
     6361 gggttggaag cgttgatacg gccggccagc ggcgcggtgt gccagcggtt gattgtggtg
     6421 tcaaactgca ggccggggtc ggcgcactcc cacgccgcct cggcaatctg ccgccagacg
     6481 tcgcgggcct tgacggtctc aatcgcctgg ccggtggtaa ccgcccttag ttcccagtcg
     6541 ccgtcatttt ccacggcacg cataaagtcg tcagtcacgc gcaccgaatt gttggcgttc
     6601 tggtactgca cggagaagct gtcgcggccg tctaagtcca tgtcgtagcc ggcctcgctc
     6661 agcacccggg ccttgcgctc ctcaatggct ttgcaccaga taaattcctc cacgtccggg
     6721 tggccaacat tcaaaatgac cattttggcc gcccggcggg tcttgccgcc gcttttgatg
     6781 ctgccggcgc tagagtcggc gccacgcata aagctaaccg gaccggagga ccggccggcg
     6841 ctgtcgccaa gtttctcctt ggatgagcgc agactggaaa gattaatgcc gctgcccgag
     6901 ccacccttaa agatcatgcc ctcctcggtg taccagttca agatggactc catagtgtct
     6961 tccacgccca gaataaagca ggcgctggcc tgctgggcgc gttccagcac accgatatta
     7021 aaccacaccg ggctgttaaa ggccgctcgc tgggtcgcca ggatgtattt gagttcttct
     7081 cggtaatctt cggcttcggc ctggctatca aagtagcctt cctgcaggcc ggcacgggta
     7141 atcgtgtccg ccaccctgtc aactaaatgc ttgagcgaac tttcgcgggt cttggtgccc
     7201 ggcgtgccgc tgaaatattt ctgggccaca atattgacgg cgttcaggct ccagccttct
     7261 ggaaattcca cgcccttttg ttcaaaaacc gctttgccgg tcgccggatt ggtgattaac
     7321 gagtcctgct tatgccattt aagctgctca taagccttac tgtccggctt ggtgaacagc
     7381 cggcggaccg ttagtttggt tgcttgagcc atagttttta ccctctctta cttgttaaat
     7441 ttttattgtt tttatcttga gctagctcct acccaggctc tcacagcggg tatgagcgct
     7501 tgctccctca gatgcttttg atattaaacc aaaacgtcac ccaaggcaac aagtctcggc
     7561 cagccttact taagattttt tattttgacg gcttcttagc agaggatttc ccggcgcttt
     7621 tgcttttgcg aattttggaa agttcgcgct caaagctact aatgtcgcgg aacttgcggt
     7681 aaacgctggc aaagcgcacg taggctacct cgttgaggtt gaccaggcgt tccatgacca
     7741 gttcgccgat gtccaccgag gcgatttccg gctcgccgcg gctgtaaagt tcgtgctcta
     7801 aatcggccac gaaagcttcc agctgcatgt gcgtcaccgc ggtcttttcg gtggcgtgct
     7861 gcaggccggc catcagcttt tcgcggctga acagctggcg gctgccatcg cgcttgatca
     7921 ccactaacat cgggcgctcc aggcgctcgt aggtcgtgaa gcggttgccg catttcaggc
     7981 actggcggcg gcggcggacg gcctcaccgt cggcgacgtc gcgcgattct atgaccttgt
     8041 tatcggttga accgcaatga ctgcatttca tgtttttacc tctgttaatg aactgtcggt
     8101 taatgtgcta tcactatatg tagtgttcgt gcaagctata ttagcgctat gagtggggcc
     8161 gtgcaagccc ttttttgtag tattaataac aaaactccta ccactgaact ctagtttttt
     8221 gacttcttag atttggcgtc atcggacttg tccggttcgg cctcgtccgg ctggtctttg
     8281 tcgtcttcag gtttcttgtc atcagcgtcc tggtcatctt cgtcagccga atcgtcagcc
     8341 ggcgctttag cagcctggcg ctcattgtcg tcctttttgg cgcggccggt cagccggcgc
     8401 aagaacgacg gtttgtccag ctcgtcttct tcctcttcct cgtcctgcgg cacgccgttg
     8461 acgtcgtcgc tcggcaatgc ccagatattg ggcgtatcgt cgctgtgaaa atcttcggca
     8521 gtggtctcta ccggctcttt gagttccatg tctaggtctt tcattgcctc atcgtccacc
     8581 tggctggtac gcgccaggct ttcctgggtg tggcggcgca gcagctcggc ggctttggcg
     8641 tcgcggctgg cataatagct gtcgtcaaag ccggtagcta ccacggtaac gataatttca
     8701 ccttccagat cgggattgat ggtggcaccg aagataacgt tggcctcaga gtcggctgcc
     8761 gccgtgatag tctcggccac ggtgttgatc tcgtgcatgc tcatgtcgtt accgccgatg
     8821 acgttgaaaa gaatgccgcg ggcgccgtca atcgcaactt ccagcaacgg tgagctgata
     8881 gcctgggtgg cggcctcaat cgcccggttc tcgccgctgg cccggccgat gcccatcagg
     8941 gcggagccgg cgtcggccat cacggccttg acgtcagcga agtccaagtt aattagcccg
     9001 tggacggtta tcagatcgga aatgccctgc acgccctggc gcagcacgtc gtcggctact
     9061 ttgaaggcct ccagcagcgg tgtgcgccgg tcaatcgtgg tcagcagttt gtcgttgggg
     9121 atggtaatca tggtgtcgac gtttttacgc atcttatcaa tcgccacctc ggcgttttgc
     9181 cggcgctttt cgccctcaaa agcgaacggc ttggtggcga aaccgacgac cagcgcaccg
     9241 gagtctttgg ctagctcggc cacgatgtgg ccggcgccgc tgccggtgcc gccgccggcg
     9301 ccgatggtca caaagaccat gtctgcgcct ttgatggcct gctcaatctc cggccgcgat
     9361 tcctcggccg cctgctggcc cttgaccgga tcggcgccgg cgcccaaacc tttagtggca
     9421 tccttaccga tgtgcacttt gttcccggcc ctgttgtggt gcagggcctg ggcgtcggta
     9481 ttaatagcta cgaaatcaac gccggtaaca ccggcgtcca ccatgcggtt aatggcggcg
     9541 ccgccggcgc cgccgacccc tactacctta atcctggcaa aagtttcaat ggctgggtca
     9601 actttgggca tgctgtttta ttctccgctt aaagctcaca aaatttgtat tcattattat
     9661 actgcagccg gcaaggcgaa tgcaatgaac aagcaccggc cggcaggcgc gccggccatg
     9721 aataaatatt tagcactgac ggcttattta cgtcgcttta accgccgcag cagtccgctg
     9781 aatgattcat taacgctttc cagcaaaccc ggctcggctt cgttaaagtg cccgccgccg
     9841 gccggcggac ccagcagcat gtccagcagc atcaggccga cacacggcgc gaagcgttcc
     9901 tcgtccaaac catccactac tttattaatg tgcccccatt tgccgacgcg cgccggcagc
     9961 tccagcacat cgcgggcgaa ttcggccacg cccggcagtt tggcggtacc gccgaccagc
    10021 accacgccgc ccggcagccg gcgcgagcga tggatgcgct tcagctcttt gt
//
LOCUS       DASPTF010000077         3883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1982514, whole genome shotgun sequence.
ACCESSION   DASPTF010000077 DASPTF010000000
VERSION     DASPTF010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982514"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1427)
                     /gene="cysS"
                     /locus_tag="VEH48_02605"
     CDS             complement(48..1427)
                     /gene="cysS"
                     /locus_tag="VEH48_02605"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXY18283.1"
                     /translation="MIRLYNTLGRKIQEFRPLEPDKVKIYTCGPTVYDYQHIGNYSGY
                     IYWDILVRLFKEQGYAVQRVMNITDVGHLVSDADEGEDKLEKSAQREGKTARQVADFY
                     TEDFYKGIDSLGLLKPDQYAKATDFIPQQLEIVQKLLDKGFAYQTEQAIYFDVSKLSD
                     YGKLTGQKLSDKEVGARAEVVTDKAKHHPQDFALWFFTVERFAEHEMRWDSPWGSGFP
                     GWHLECSAIIHAVLGEPIDIHTGGVDHIGTHHTNEIAQTEAAFDKSLADLWLHNNHMM
                     VDGQKISKSLRNGYTIDDLAKKGFSATDFKLLVLQSHYRSQMNFKWEALEAAQANLAD
                     LHAWADQTYQLGVNALSSEQEEALIKIVKGSLAEDLNTTVALASVNGITLKGRPSRPL
                     LRQLDELLGLGLSERQDITSEQKQVIEQREQARRAGNWPQADELRQSLKRQRLEINDT
                     DAGPVWSRA"
     gene            complement(1424..2434)
                     /locus_tag="VEH48_02610"
     CDS             complement(1424..2434)
                     /locus_tag="VEH48_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HXY18284.1"
                     /translation="MVEYPEAKKIAELVAQAGSILVIQADNPDADSLASALALEHILE
                     EAGKQITLYCGVDLPSYLSYLPGSDRVSSSLPNNFDLSIIVDTSSIALLEQLEKQGAK
                     GWLAAKPCIILDHHASQPTIDFASVVCNHPAVATAEIIYELALQLDWPLNLDAKKLLA
                     MAILSDSLGLVSEATTYRSVEIIAELVKAGVKLSEIENLRRESMRRDPELIHYKGRLL
                     ERVEFHGAIAIVTIPWEEIEKFSPLYNPPMLVLDDMRLAKGTAVAVAFKLYNDGKITA
                     KIRCNYGSAIADKLAEHFGGGGHPYAAGFKLTDGRSYEEIKNETIRTASELLETPAKA
                     DA"
     gene            complement(2466..3071)
                     /gene="recR"
                     /locus_tag="VEH48_02615"
     CDS             complement(2466..3071)
                     /gene="recR"
                     /locus_tag="VEH48_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXY18285.1"
                     /translation="MTVIPKPLQDVIEAFGLLPGVGPRTAERYAYYLLRNRPEKALAL
                     AESLTRLHAGVSYCPVTFALIERGQKVSPLYSDPKRNKQVVAVVADPFDIVALEKTNS
                     FRGTYHVLGGLVSPIDGIGPEQLHINELLKRIDKDKVKELILATNASVEGESTALLIS
                     QQIGKRPVKITRLARGLPVGVDLEYADQITLTRALEGRQKI"
     gene            complement(3088..3381)
                     /locus_tag="VEH48_02620"
     CDS             complement(3088..3381)
                     /locus_tag="VEH48_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXY18286.1"
                     /translation="MSKFDQAKLLMKARKLQKELQKEIITIETGDGAVKVEISGEQKI
                     KRIQIDPEAVDLDDIRQLEMWLEEAVKEAIVRSQQLAAEKMQPMMGALGQLGL"
     gene            complement(3581..>3883)
                     /locus_tag="VEH48_02625"
     CDS             complement(3581..>3883)
                     /locus_tag="VEH48_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18287.1"
                     /translation="ARRTMLGILAIIVFSIIAAAINNNVLLLALGLPGLAVLVGGGLR
                     YLYAEWRSVFPRNPIPLGFAWALIIAVVGLQMAYGLRYSLIAWPHAPATKQTYVLK"
BASE COUNT          835 a         1038 c         1002 g         1008 t
ORIGIN      
        1 aataaggttg cccccactga agacctaggt ataacaacag cttgatttta agcccgcgac
       61 caaaccggcc cggcatccgt atcattgatt tctaatctct gccttttaag actctgccgt
      121 aattcatccg cctgaggcca attgccagcc cttctggcct gttcacgttg ctcaataact
      181 tgtttctgct cagatgttat gtcttgcctt tccgataaac ccaaacccaa gagttcatca
      241 agttgcctca aaagaggtcg tgatgggcga ccttttaagg tgatgccgtt taccgatgcc
      301 agagcaacgg tcgtgttcag gtcctccgcc aaagaacctt taactatttt aattagtgct
      361 tcctcttgct ctgacgacaa tgcgtttacg ccgagctggt aggtttgatc tgcccatgcg
      421 tgtaaatcgg ctaaattggc ttgggctgct tcaagagcct cccacttaaa attcatctga
      481 ctacgatagt ggctttgcag aaccagtagc ttaaagtctg tagctgaaaa accctttttc
      541 gccagatcat cgatagtgta gccattacgg agggacttgc tgattttctg accgtcaacc
      601 atcatgtggt tattgtgcag ccataagtcg gccaatgatt tgtcaaaagc agcctcagtc
      661 tgggctatct cattagtatg gtgggtgccg atgtgatcga caccgccggt atgaatgtca
      721 atcggctcgc ccaagacagc atggattatt gccgaacact ccagatgcca gccgggaaaa
      781 ccgctgcccc acgggctatc ccagcgcatt tcatgttcgg caaaacgttc aacggtaaag
      841 aaccatagag caaaatcttg tggatgatgt ttggccttgt cggttaccac ttcggctcgg
      901 gcgccaactt ctttatctga cagcttttgt ccggttagct ttccgtaatc gctgagttta
      961 gagacatcaa aataaatcgc ttgctccgtt tggtatgcga atcccttatc cagcagtttt
     1021 tggacaattt ccagctgctg cgggataaaa tccgtagctt tagcgtactg gtcaggcttc
     1081 agcagtccaa ggctgtctat gcctttgtaa aaatcttcgg tataaaaatc ggctacctgg
     1141 cgagcggttt tcccttctcg ttgggcgctt ttttccagtt tgtcttcacc ttcgtcggca
     1201 tcgctcacta ggtggccaac gtcagttatg ttcatgacgc gctggacggc atatccctgc
     1261 tctttaaata gccggaccag tatatcccaa taaatatagc cgctatagtt gccgatgtgc
     1321 tggtaatcgt aaacggttgg accacaggta tagattttga ctttgtcagg ttccagaggg
     1381 cggaattcct gaattttacg ccccaaagtg ttgtaaagcc tgatcacgcg tcagcctttg
     1441 ctggagtttc caatagctcg gaagcggttc tgatagtttc gttcttgatt tcttcgtaac
     1501 tgcggccgtc ggtcagcttg aaaccggcgg cataagggtg gccgccgccg ccgaagtgct
     1561 cggccagctt atcggcgatg gcgctgccgt aattgcagcg gattttggca gtgattttgc
     1621 cgtcgttata gagcttgaaa gctaccgcca cggctgtgcc tttggccagg cgcatgtcgt
     1681 ccagcaccag catcggcgga ttatacaagg ggctgaactt ttcaatttct tcccagggga
     1741 tggtcacaat agctatggcg ccatggaact ccactcgctc cagcaagcgg cctttgtagt
     1801 ggatgagctc cgggtcgcgg cgcatggact cgcggcgcag attctcaatt tccgatagtt
     1861 tgacaccggc tttcaccagc tcggcgatta tttccacact acggtaggtc gtggcttcgc
     1921 tgaccagccc caggctgtcg ctcaagatgg ccatggccag caattttttc gcgtccagat
     1981 tgagtggcca gtccagctga agagccagct cgtaaataat ctcggcggtg gcgacggctg
     2041 gatgattgca gacaaccgaa gcgaaatcaa tggtcggctg gctggcgtgg tggtccagga
     2101 taatgcacgg tttggccgcc agccagccct tggcgccctg tttttccagc tgttccagca
     2161 aggcgatact gctggtatcc acgatgatgc tgagatcaaa attgttaggc aggctgctac
     2221 tgacgcggtc agagcccggt aaataactga gataactcgg cagatcaacg ccgcagtaaa
     2281 gtgttatctg cttgccggct tcttctaaaa tatgctccag cgccaaggcg ctggccaggc
     2341 tatccgcgtc cgggttgtcg gcctgaataa ccaatataga accagcctgg gctaccagct
     2401 cggcgatttt cttggcttcg ggatattcaa ccatcagcct tattttaaca gtttaggccg
     2461 ctgatttata tcttctgacg gccttccagc gctctggtga gagttatctg gtcggcatac
     2521 tccaaatcca cgcccaccgg caagccgcgc gccagacgag tgattttgac cggccgcttg
     2581 ccgatttgct ggctgatcag cagcgcggtg ctctcgcctt ccacgctggc gttagtggct
     2641 aaaatcagct ctttgacttt gtctttatca attcgtttaa gcagttcatt gatgtgcaat
     2701 tgctccgggc cgatgccgtc tatcggcgag accagcccgc ccaaaacgtg gtatgtaccc
     2761 ctgaaactat tggtcttttc caaggcgacg atatcaaacg ggtcggctac caccgccact
     2821 acttgcttgt tgcgtttagg atcactataa agcgggctta ccttttggcc gcgctcaatc
     2881 agggcgaaag tgaccgggca gtaggacacg cctgcgtgca gcctggtcag agattcggcc
     2941 agcgctaaag ctttttccgg ccggttacgc agcagataat aggcgtagcg ttcggcggtg
     3001 cgcggcccga cgccgggcag cagcccgaaa gcctcaatta catcttgcag cggtttgggt
     3061 attacggtca tagacccctt aaaagtttta cagtccgagc tggcccagcg cgcccatcat
     3121 cggctgcatt ttttcggccg ccagctgctg gctacgtaca atggcctctt tgactgcctc
     3181 ttccaaccac atctccagct gtcggatgtc gtctaaatct accgcttcag ggtctatttg
     3241 gatacgcttg attttctgct ctccgcttat ttccactttg acggcaccgt ctccggtctc
     3301 aatggttatg atctctttct gcagctcttt ttgcagcttg cgggctttca tcaagagctt
     3361 ggcttggtca aacttactca tattaactcc tctatattcc ttatatctta tcacaacctt
     3421 ctcgcttgga tgcgatacaa ggaagcaatg agtagcgcaa tgagacgtat taaaatacgt
     3481 tgaatgagca gcggaattgc tgacgccgta acgcgccaag agctttagcc gtgcagccgg
     3541 ctcggccggg tggcaggcta aagcggcgtg aaggttgtgg ttattttagc acgtatgtct
     3601 gtttggtggc tggggcgtgc ggccaggcga tcaaactgta gcgcaatccg taagccatct
     3661 gcaggccgac caccgcgatt atcagggccc aggcaaagcc caacggtatg ggattgcgcg
     3721 ggaagacact gcgccactcg gcgtacagat agcgcaggcc gccgcccacc agaaccgcca
     3781 ggcccggcag gccgagcgcc agcagcagga cgttattgtt gattgcggcg gcgatgatac
     3841 taaagacaat gatggccaag ataccgagca tcgtacggcg ggc
//
LOCUS       DASPTF010000078        13685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_713674, whole genome shotgun sequence.
ACCESSION   DASPTF010000078 DASPTF010000000
VERSION     DASPTF010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 13685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13685)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13685
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713674"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VEH48_02630"
     CDS             <1..270
                     /locus_tag="VEH48_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18288.1"
                     /translation="RPKDVRDKTSAGLQVMTAFAVHPNYPSAIDYDAIPGAWAAGLQA
                     SIPGREAWTDVPILSWSRTDREVSLTPHWDGNRDPDYAVPRVREL"
     gene            complement(436..1935)
                     /locus_tag="VEH48_02635"
     CDS             complement(436..1935)
                     /locus_tag="VEH48_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXY18289.1"
                     /translation="MFKFLAKLYQKPLDALDGLLDGLTSYKLVLYYLLVLVAWAIVVS
                     FRKSVPFSWHAIGLSAILLLAVCRASGELFSRVLNIARNFESDYITALILALIMSPAT
                     TGKGYAVLVGAGFAAIASKYLLTINRRHVFNPAAFGAVVAGIFFHQYASWWVGTSALV
                     PLVLVGGVLIMRKMKRFLMVIVFFLVYGFFLHQQFDGAAPVHTIWLAATGTGILFFAY
                     VMLLEPQTSPDKFRNYFLYSVLVGIFYGWTRLGISPEESLLIGNVLAYALERGKLLEL
                     RLERVQKEAAGLYSFSFSHQGTFDYKPGQFMEWTLPLRHSDSRGNRRYLTLSSSPTEN
                     LLSFTVRLPQKASHFKQALAKFKPGDKILASHLGGSFVLPSDKRQKLAFVAGGIGVTP
                     FRSIAKYMIDKKDERDATLLYCANGAAEFAFQDLFSGAKNLGLAAHYIDTSTAPISAD
                     IIANAVPDFKQRLFYISGPYGFVKSVRQALLSLGIGPTNIKSDYFPGYG"
     gene            complement(1983..3164)
                     /locus_tag="VEH48_02640"
     CDS             complement(1983..3164)
                     /locus_tag="VEH48_02640"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXY18290.1"
                     /translation="MESTKRKVLVLGGGFGGIKAALELAGHRRYSVALLSDHPDFRYY
                     PTLYRTATGGSRQASSIPLAEIFRDKEVRLIDDRVKKLDRDQKTVSGASGKSYKYDIL
                     IIALGVVTNFFGIRGLDKYAFGIKTNEEARRLRNHLHQQLADDKKPDLNYVIIGGGPT
                     GVELAGALPGYLRHIMHRHGISQRAVHVDLVEAAPRLVQRMPKTYSRAIERQLRKQGV
                     KLYLGQSVQAETADALMVSGHSIASHTVIWTAGVTNHPFLKAGGFDLTDHGKALVDRY
                     LSASPDVYVIGDNADTPYSGMAQTALYDGRFVAANLKRMAEGRLPKPYRSKTPIYVTP
                     VGPRWAAVLWHGLHLYGWLGWALRGAADFLGYHDYEPWWSAGKHWMAEARKEEECPIC
                     T"
     gene            3277..4731
                     /locus_tag="VEH48_02645"
     CDS             3277..4731
                     /locus_tag="VEH48_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXY18291.1"
                     /translation="MARETRELSKHSDNKWVILALLAMAQFMVVVDVTVVNVALPSMA
                     RLLHFSASNLQWVITAYTLTFGGFLLLGGRASDLFGRRRIFVGSVFFFALMSFLCGMA
                     ATDTQVIIARAAQGLAGAIMSPAALSIVISEFKEGKERNTALGVWAGVAAGGSAVGLL
                     LGGLLTQYLSWRWNFFVNVPIGLGVVYASIKLLPHHRGEEHHNVKLDLPGAVLATGGL
                     MSLVYGLSKAPADGWHSATVWGFIATSVVLLIAFVFNERRTDQPLMPLSMFRIRNVSG
                     GNATALAIACTLFSMFFFMTLYVQTVLGYGPAKSGVSFLPVTFVIGATSAFVSKMVNK
                     VGYKPFLVAGPVVLSIGLYTISTLMKVGGNYWHNVFPGLVLIALGMGFSFISMTLAAT
                     TGIPRTLSGIASGVLNTSQQVGGAIGLAILSAVYASTLRVDTTRGVALNYAKIHGFRD
                     GLHVGVGLAVTAAIVAFVVVHNEKSSSQLSSTSA"
     gene            complement(4721..5134)
                     /locus_tag="VEH48_02650"
     CDS             complement(4721..5134)
                     /locus_tag="VEH48_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HXY18292.1"
                     /translation="MYDNLRALLKKSGASLTKPRKTVFDLLRDQEPQSMRVLAQRAAG
                     KVDRATVYRTVELFERLGIVHRLNIGWKYKIELSDVFHSHHHHFYCTNCNKTFPLPAN
                     SMLEAMIDSTVAKEGFAPRGHQLEIFGLCSSCSRR"
     gene            5205..5930
                     /locus_tag="VEH48_02655"
     CDS             5205..5930
                     /locus_tag="VEH48_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HXY18293.1"
                     /translation="MIVFLLTVAASIAALAGGYTALRSRKWLNWALALTAGLVIGLVF
                     FDLMPEIFDIAVRQNLDTAWPMVALVSGFLLFHVFEKFVPIHQASEEQYAAHRHPRLG
                     TARAVALSGHSFMDGLSIGVAFQVSSAVGAAVALAVIGHRFADGFDTTTFMVFHHNKL
                     VHIKRWLAVVVLMPIFGGLLSLVYDFPETVLALYLGFFAGFILYIAASNILPQAHSEE
                     FSRKSILLTVFGTVFMFVVTRFV"
     gene            5947..6582
                     /locus_tag="VEH48_02660"
     CDS             5947..6582
                     /locus_tag="VEH48_02660"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXY18294.1"
                     /translation="MMKIKIALLVVVVAAAGFFVYRHQNTAEQVTQKAAATAKAATAK
                     ADAAKAAAEELASHCAGNGAGQEVIVSISKQHLWACDSTKVAYDSAVITGMEMYPSEL
                     TPVGTYKIYDKLTDQTLTGSDFTGHWSDYVYYWMPFLQNQYGTYGFHDLTQTVNGTTV
                     GRADSDFGNIDINAPYTAAKHGSHGCVEMPLAAAAWLYNWAPIGTTVSVQS"
     gene            complement(6579..7304)
                     /locus_tag="VEH48_02665"
     CDS             complement(6579..7304)
                     /locus_tag="VEH48_02665"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HXY18295.1"
                     /translation="MESIDLARLTNSLKQHTAVHAAWLEGSRGEGTNDEFSDMDIWVD
                     VDDGREEEVYEHIESFLRKIGELDVDYAANIAHPLLRHRTYHIAGTNPFERLEINMQS
                     HSRNFRFTEGVHNIKVLFDKDSTIKMERLNKEALEKELASRKRFLLEKIEFGELWVEK
                     EIRRRKFLEALGMYNHFFAESIVELCRIKYSPYKISHGLKHIYTDLPQDIVRKIESIH
                     QITSIEDIKEKITEVKDLAKELF"
     gene            7356..8414
                     /locus_tag="VEH48_02670"
     CDS             7356..8414
                     /locus_tag="VEH48_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXY18296.1"
                     /translation="MIVAVIVLAVLLVVMVGLLVWQNLRPKKQSDSALLLKADMTELT
                     KAMGQLQQGLQKQLTEQLGTSNRQMAAQFQASTKIIKDVTEKLTELDRTNKSVGDVAA
                     ELKTLQNVLQNPKQRGVLGEYYLDQILKNLLPPGTYELQYKMGEGLTVDAVVKLDDKL
                     LPIDSKFSLENYNRLLDAKPEERPALERAFKEDLKRRIDETAKYIRPNKGTLDQALMF
                     IPSEAIYYDLLANKVGAAGVSGRNLMQYAAKDKKVTIVGPSTLSAMLQVIVQGLRSIE
                     IQKDTDKIRKNIEQLSKHLVAHNGYMQKLGASLGTTVGHYNATYKELGKIDKDVVKIA
                     DTEPSVEPILIDRPNLDD"
     gene            complement(8416..8934)
                     /locus_tag="VEH48_02675"
     CDS             complement(8416..8934)
                     /locus_tag="VEH48_02675"
                     /inference="COORDINATES: protein motif:HMM:NF027223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase
                     domain-containing protein"
                     /protein_id="HXY18297.1"
                     /translation="MSIRVHRPPEIQNSDGPGIFLAGPIQGAPNWQADAVNHFEDLLK
                     NEATVNIFNPRASSLDHTKEDQILWEKKYLLHARRYGAIMFWFAAQDHSIKDYPVGRA
                     YAQTSRIELGRAIGWKDYAYLIRLGIGIDPDYTGGNEDYIKSCAKEHKIPVYDSLARL
                     CTHVVELCQTEM"
     gene            complement(8973..10295)
                     /locus_tag="VEH48_02680"
     CDS             complement(8973..10295)
                     /locus_tag="VEH48_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HXY18298.1"
                     /translation="MAEEQTRLLQEAEKVLKHNDRGKWVMPAANLYPHQWLWDSCFIA
                     IGLRHLNVERAQTELKSLLRGQWSNGMLPNIIFSGAKQYRRDRELWRSWVSPYSPSQV
                     ATSGITQPPMLAEAVLQIGQKLKLPERRSWYQQMYPALLHYHEWLYNERDPHHEGLIV
                     LLHPYEGGLDDSPPWISELRKHNMPLWLTAAEKARLGSLIQFFRRDTQHTTLAQRMSN
                     IEAAAYWAAMHRLRRKAYNSEAILSRSLFSLEDLAFNCIFIRANDCLKKIAADIKQEL
                     PPALLKNMANTESALEQLWDESSGQYFSRSFVSHKLIEESSVATLLPLYAGSISKERA
                     ARLVELLQDKKWFASNWPVPSVPRNSPYFEPCRYWQGPAWINTDWLIIKGLENYGFSK
                     EAASLRQKSLDLVAKSGFYEYFNPLTAEPAGAQNFSWTAALIIDLLKS"
     gene            10367..11392
                     /locus_tag="VEH48_02685"
     CDS             10367..11392
                     /locus_tag="VEH48_02685"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXY18299.1"
                     /translation="MEYKNSEIAKAAKQLKDKLAKLDDKRAILRAPELAALYERLKTL
                     PAGKPRADFGKQVNALRNELEQLIRDSLPTTRHPLPPIDVTAPFDANVKPEDRPDLLP
                     AKSGSIHPINTELKIILDIFGRMGFEVVESREIDDDYHMFGSLNFPPGHPARDEYDTF
                     VTTEGLIAPAHTSIMQNRVLKTRKPPIRAVIPGRVFRNEDLDARHEHTFYQLEGVYVD
                     EGINAGHLVATIQAWLSNYFERDVNIKIQPFYFPFTEPSFEFAASCPFCAGKGCSVCS
                     HTGWIELGGCGMIHPNVLKEGGVDAKKYTGFAWGFGIERLVMLKYNIEDVRLFESANL
                     KFLRQFE"
     gene            11389..11964
                     /locus_tag="VEH48_02690"
     CDS             11389..11964
                     /locus_tag="VEH48_02690"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXY18300.1"
                     /translation="MKNAILIHGWNTKEEYYDPVRPTASNDHWFPWLTKQLVLKDINT
                     VSVEMPDGYYPEYHIWKRELERYDISSKTTLVGHSCGGGFLVRWLSENDVKVGKVILV
                     APWLGLDTQGEPFDKSFFDFEIDPKIAEKTAGLSILISDDDYRDVLESVEILRKKLDG
                     VNIKEFHGKGHFVLSSLGGPEFPELLEEILK"
     gene            11961..>13685
                     /gene="pheT"
                     /locus_tag="VEH48_02695"
     CDS             11961..>13685
                     /gene="pheT"
                     /locus_tag="VEH48_02695"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXY18301.1"
                     /translation="MKVSLNWVKEFTSVDLPVDELVEKIGAQLGAVEEVEEIGKKYQG
                     ILVVKVVLAEKHPNADRLTFCLVDDGKANKKVKRNKDGLIEIVCGAPNVKTGQLAVWI
                     PPGATVPATYDKEPFVIEAREIRGKTSHGMLASLKELALGDSHNGIVVLDMGKPGDSF
                     AKTLWLDDYIIDIENKMFTHRPDCFGMLGVAREIAGIQHKAFQSPDWYRENASLPTDG
                     RKNVLKITVKNDVPKLVPRFCAVAIKDVKVEPSPAWMQSFLARVGVRPINNIVDITNL
                     VMLETAQPIHAYDYDKLKTGVLGVRLSKKGEQLKLLGGKTIKLDDGAVVITDGKQPVG
                     LGGVMGGAGTEVDENTKNIVLEVANFDMNLTRRTAMTYGLFTDAAVRFTKGQSPDQNM
                     AVLCRAVNHALQFAGGRQASAVVDDFAKQETRNKKQEIVVGAEFVNSRLGLNLSGAEI
                     KKLLENVEFKVELSGGSLKITPPFWRMDIAIPEDIVEEVGRLYGYDKLPVVLPPRDLS
                     PVQPNPRVVFKDRLRDILSRAGANEVLTYSFVHGSLMQKVGQDPKDAYRIANALSPDV
                     QYYRLSLTP"
BASE COUNT         3310 a         3554 c         3589 g         3232 t
ORIGIN      
        1 cgtcccaagg acgtgcgcga caagacgtct gccggcctgc aggtcatgac ggcgttcgcg
       61 gttcatccga actacccgtc ggccatcgac tatgacgcca tccccggcgc ttgggcggct
      121 ggccttcagg ccagcattcc cggccgcgag gcttggacgg acgtgccgat cctctcctgg
      181 agcagaacgg accgcgaggt cagcctgacc ccccactggg acggcaaccg cgatccggac
      241 tacgccgttc cgagggtcag ggagttgtag tattgaggac ttgcgcctct tggagtttgg
      301 agggccttgg gatttttccc ttggccctcc agttccgctc atcttgggca cgaacgctcg
      361 cgataaaacc gctagagcac agagtttcgt gcccgttttc atatccgtaa actgtcaaaa
      421 aactaggtgg atattctagc cgtagccggg gaagtaatct gacttgatat tggtcgggcc
      481 gatccccaag ctcagcagtg cctggcgcac gcttttgacg aagccgtaag ggccggagat
      541 gtaaaataac cgctgcttga agtccggcac ggcattggcg atgatgtcgg cgctgatcgg
      601 cgccgtcgag gtgtcgatgt agtgcgcggc cagaccgaga tttttagccc cgctgaataa
      661 gtcctggaaa gcgaactcgg cggcgccgtt ggcgcagtac agcagggtcg catcgcgctc
      721 atctttctta tcaatcatgt acttggctat gctcctgaaa ggcgtgacac cgatgccgcc
      781 ggccacgaat gccaattttt gccgcttgtc ggacggcagg acgaacgatc cgcccaagtg
      841 cgaagccaaa attttatctc ctggcttgaa ctttgccagt gcctgtttaa aatggctggc
      901 tttttgcggc aagcgtacag taaaagatag caggttttca gtcggtgacg aagacagggt
      961 caaatatcgc cggttaccgc ggctgtcgct atgacgcagc ggcagcgtcc actccataaa
     1021 ttggccgggc ttgtaatcga acgtaccttg gtgggaaaaa ctgaagctat aaaggccggc
     1081 ggcctctttt tgtacccttt ccaggcgcag ctccaggagc ttgccccgct ccagcgcata
     1141 agctagcacg ttacctatca acaacgattc ctcggggctg atccctagcc tagtccagcc
     1201 ataaaagatg cccactaaaa ccgaataaag aaaataatta cgaaacttgt cgggtgacgt
     1261 ttgtggctcc agcagcatca cgtaagcaaa gaaaagtatg cctgtaccgg tggcagccaa
     1321 ccatatggtg tgtaccggcg cggcgccatc aaattgctgg tgcaagaaaa acccgtaaac
     1381 caggaaaaat acaataacca ttaaaaaacg cttcatcttg cgcattatca atacgccgcc
     1441 aaccaacaca aggggtacca gggcagaggt gccgacccac caactggcgt attggtggaa
     1501 aaatattccg gccaccaccg cgccgaaggc cgccgggtta aaaacgtgcc ggcggttgat
     1561 ggtcagaaga tacttggaag caatggccgc aaagccggcg ccaaccaaaa cggcatatcc
     1621 cttgccggtg gtggccgggc tcattatcag ggctagaatc aaagcggtga tgtagtccga
     1681 ttcaaaattg cgcgcgatat ttaatacccg cgaaaaaagt tcgccgctgg cgcggcaaac
     1741 agctagcaat aaaatcgccg acaggccgat ggcgtgccag ctgaacggca cgctttttct
     1801 gaagctgacc acgatagccc aagccaccag caccagcagg tagtaaagga ccaacttata
     1861 agaagtcagg ccgtccagca gaccgtccaa cgcgtctagc ggcttttggt agagtttggc
     1921 gaggaatttg aacatatcag gccattaaag cttagcaagc tgagttttag ctaagaaaca
     1981 gctcaagtgc aaatcgggca ttcttcctct ttgcgggctt cggccatcca gtgtttgccg
     2041 gcgctccacc acggctcgta atcgtggtag ccaaggaaat cggccgcgcc gcgcagagcc
     2101 cagcccagcc aaccgtaaag atgcaggccg tgccacagca ccgccgccca gcgtggcccg
     2161 acgggggtga cgtaaatagg ggttttggag cggtacggct tgggcagccg gccttcggcc
     2221 atgcgcttaa ggttagcggc cacgaaacgg ccatcataaa gcgccgtctg ggccatgccg
     2281 gaataaggtg tatcggcgtt gtcaccaatc acgtagacgt ccgggctggc gcttaagtag
     2341 cggtctacca gcgctttgcc gtggtcggta aggtcaaagc cgccggcttt caaaaacggg
     2401 tggttggtaa cgccggcggt ccaaattacg gtgtggctgg cgatggagtg gccgctgacc
     2461 atcagcgcgt cagcggtctc ggcctgcacg ctctggccaa gataaagctt caccccctgt
     2521 ttgcgcagct gccgctcaat ggcccgcgaa taggtctttg gcatgcgctg caccaaccgc
     2581 ggcgcggctt ctaccagatc aacgtgcacg gctcgctggc taatgccgtg gcggtgcatg
     2641 atatgacgca aatatccggg cagcgcgccg gccagctcca caccggtggg cccgccgccg
     2701 attatcacgt agttcaagtc aggctttttg tcgtcggcta actgctggtg caagtggtta
     2761 cgcagccgac gggcttcttc attagtctta atgccaaaag cgtatttgtc caaacctctg
     2821 atgccgaaaa aattcgtcac cacacccaga gcgattatca gaatgtcgta cttgtaagat
     2881 ttgccggacg cgccgctgac tgttttttgg tcgcggtcca gctttttgac cctgtcgtca
     2941 atcagccgca cttctttgtc cctgaaaatt tcggccagcg ggattgaaga agcctggcgg
     3001 ctgccgccgg ttgccgtacg gtacaaagtt gggtaatagc ggaaatccgg gtggtcggaa
     3061 agcagcgcta cgctgtagcg ccgatgcccg gccagctcca atgcggcttt gataccgccg
     3121 aacccgccac ccagaaccaa aactttccgt ttggttgatt ccatttatgc ttattttagc
     3181 ggccggccgg caaaaatgcg aactttggtt tggccatacc gcccggtttt gttaacatta
     3241 caggacacgc aatttataaa aagagggagg tccaacttgg cgcgagaaac aagagaatta
     3301 tcaaaacact ctgataacaa atgggtgata ttggccctgc tggcgatggc gcagttcatg
     3361 gtggtggttg atgttacagt tgttaacgtc gccttgccgt caatggcccg actgctgcat
     3421 ttttccgcca gtaacttgca gtgggtaatt accgcttata ccttaacctt tggcggtttc
     3481 ctgcttttgg gtggccgcgc gtctgatctt tttggccgcc gaaggatttt tgtcggctcg
     3541 gtatttttct ttgcgctgat gtcattcctt tgcggcatgg ccgccaccga cacccaggtg
     3601 attattgcgc gggctgccca gggcttggcc ggagcaatca tgtcgccagc ggctctatct
     3661 atcgtgatta gcgaatttaa agagggtaaa gaacgcaaca ctgctttagg tgtgtgggcc
     3721 ggagttgctg ccggaggctc ggcggtcggg cttctcttgg ggggtcttct gactcaatac
     3781 ctgagctggc gctggaactt ctttgttaac gttccgattg gtcttggggt ggtctatgct
     3841 tctatcaaac ttctgccgca ccaccgtgga gaggagcatc acaatgtaaa gttggattta
     3901 ccaggcgccg tattagccac cggcggttta atgagtttgg tatacggatt gtcaaaggcg
     3961 ccggctgacg gttggcattc cgcaacagtg tggggtttca ttgccacttc ggtcgttctg
     4021 ctgattgcat ttgtcttcaa cgaacgacgc acagaccagc ccttgatgcc gcttagcatg
     4081 ttccgtatcc gtaatgtctc cggcggtaac gccacggcgc tggccatcgc ctgtactttg
     4141 ttctcaatgt tctttttcat gacactctat gtccaaacgg tactcggtta tggaccagct
     4201 aaatccggcg tgtcattctt gccggtcacc tttgtcattg gcgcaacctc cgctttcgtg
     4261 tcaaaaatgg tcaacaaggt tggctacaaa ccgttcctgg tcgccgggcc ggtcgtgctg
     4321 agtatcggac tatataccat tagtaccctg atgaaagtcg gcggcaatta ctggcacaac
     4381 gtcttccccg ggctggtatt gattgccctg ggaatgggct tttcgttcat ttccatgacc
     4441 ttggccgcga ccacagggat acccaggacg ctttccggca tcgcgtcggg cgtcttgaac
     4501 accagccagc aggttggtgg agcaatcgga ctagctatct tgtcggcggt ttacgcctca
     4561 acgcttagag ttgatacaac ccgcggagta gccttgaatt acgcgaagat tcacggtttc
     4621 cgcgatggtc ttcatgtagg agttggactg gcggttaccg ccgcgattgt agctttcgtg
     4681 gtcgtacata acgaaaaatc aagctcccag ctatcttcca ctagcgccta gagcaactgc
     4741 tgcacaggcc aaaaatttcc aattggtggc cgcgcggcgc aaaaccttct ttggcgaccg
     4801 tactgtctat catggcttcc agcatagaat tagcgggcaa ggggaatgtc ttattgcaat
     4861 tggtgcagta aaagtggtgg tggtggctgt ggaagacatc gctaagttca atcttatatt
     4921 tccagccgat gttcagccgg tggacgatac ccaggcgttc aaacagctcc actgtgcggt
     4981 aaacggtggc acggtctacc ttgccggcgg cccgctgggc cagtacccgc atactctgcg
     5041 gctcctggtc gcgcagcaga tcaaaaacgg tcttgcgcgg cttggttaag ctggcgccac
     5101 tctttttcag caatgcccgg agattatcgt acatttctaa cgcaatttta aagaaattgc
     5161 gacaaatttg caaataatag caaaacagaa atagaatcaa ccaaatgatc gtgttccttt
     5221 tgactgtggc ggcttcgatt gccgcgctgg ccggcggcta cacggcattg cgcagccgta
     5281 aatggctaaa ttgggcattg gcgctgaccg ccggtctggt aattggcctg gtgttttttg
     5341 acttgatgcc ggagattttt gacattgccg tccgccaaaa cctcgacacc gcttggccga
     5401 tggtggcgct ggtttccggc ttcctgctat tccatgtctt tgagaaattc gtgcccattc
     5461 accaggccag tgaagagcag tatgccgcgc accgccaccc gcgactgggc actgcccggg
     5521 ccgttgcatt atctggccac agtttcatgg acggtttgag tatcggcgtg gcattccaag
     5581 tcagttcggc tgtcggtgcg gccgtggctc tggcggtaat cggccaccgg tttgccgatg
     5641 gctttgatac cacgactttc atggtttttc atcacaataa gttggtgcac atcaaaagat
     5701 ggctggcagt cgtagtcctg atgccgatct ttggcggcct gctaagcctg gtatatgatt
     5761 tcccggaaac ggtactggcg ctttatcttg gtttttttgc cggctttatt ctttatatcg
     5821 ccgccagcaa cattctgccg caggcccaca gcgaggaatt ttcgcgcaaa agtatactgc
     5881 tgacagtttt tggtacggtc tttatgtttg tcgtcacccg atttgtctag gtataattag
     5941 gctgcgatga tgaaaataaa aatcgccttg cttgtagttg tagtcgccgc tgccggcttt
     6001 tttgtctatc gccaccaaaa tacggccgaa caagtaacgc aaaaagccgc cgccacggcc
     6061 aaagccgcca cggccaaggc cgatgccgcc aaggccgcgg ccgaagagtt agccagccac
     6121 tgcgccggca atggtgcggg acaagaagtt atcgttagca tcagcaaaca gcatctatgg
     6181 gcctgtgaca gtactaaggt tgcatatgac agcgctgtaa tcaccgggat ggagatgtat
     6241 ccttcggagc tcacgccggt gggaacttac aagatatacg ataaattaac cgatcagaca
     6301 ctcaccggtt cggattttac cggccactgg agtgattatg tctattactg gatgccgttt
     6361 ttgcaaaacc aatacggcac ttacggcttc cacgacctga cacaaacagt taatggcacg
     6421 accgtcggcc gggctgacag cgattttggc aacatcgaca tcaatgcgcc gtataccgcg
     6481 gctaaacacg gcagccacgg ctgcgtagaa atgccgctag ccgccgccgc ctggctctac
     6541 aactgggctc ccatcggcac aacagtctct gtccaaagct aaaaaagttc tttggctaaa
     6601 tctttaacct cggtaatctt ttctttaata tcttcgatag acgttatttg gtgaatcgat
     6661 tcaatctttc ttactatatc ctggggtaaa tctgtataaa tatgtttaag tccgtgggag
     6721 attttataag gggagtattt aatcctgcat aactcaacaa tcgattctgc aaaaaaatgg
     6781 ttgtacatac ccagtgcttc tagaaatttc cgtcgtctaa tttctttttc gacccatagc
     6841 tctccgaatt cgatcttttc tagcagaaat cgttttctgg atgcaagctc tttctcaaga
     6901 gcttctttat taaggcgttc catttttata gtcgagtctt tatcgaaaag cacctttatg
     6961 ttgtgaactc cttcagtaaa cctaaaatta cgggaatgtg attgcatatt aatctcaagc
     7021 ctttcaaaag gatttgtgcc agcaatatga taagtcctgt gtctcagtag cggatgagca
     7081 atgttagctg cataatccac atcgagttcg ccaatcttcc tcaagaagct ttcaatatgc
     7141 tcataaactt cttcttcgcg gccgtcgtca acgtcgaccc atatgtccat gtccgagaat
     7201 tcatcatttg ttccttcacc tcttgaaccc tccagccagg ccgcatgaac cgccgtgtgt
     7261 tgtttcaaag agtttgttaa tctagccaag tctatagatt ccacactaag attgtacacc
     7321 gatgaccata attcgcttta aggtacaata aagatatgat tgtggcggtg attgttttgg
     7381 cggttttact ggtggtaatg gtcggtttgc tagtttggca gaacttgcga ccgaaaaagc
     7441 aatcggattc agcgctgctg cttaaggccg atatgaccga actgactaag gccatgggcc
     7501 agctgcagca aggtcttcaa aaacaactca ctgagcaatt gggcacctcc aacagacaaa
     7561 tggccgccca gttccaggcc agcaccaaaa tcatcaagga tgttacagaa aaattgaccg
     7621 agctggaccg cactaacaaa agtgtcggcg atgtggcggc cgaactcaaa accctgcaaa
     7681 acgtgctgca aaatcccaaa cagcgcggcg tactgggcga gtattatctt gaccaaatcc
     7741 tcaaaaatct actgccgcca ggcacttacg aactgcagta caagatgggc gagggcctga
     7801 cggttgatgc ggtcgtcaaa cttgatgaca aactgctgcc aatagacagc aaattcagtt
     7861 tggaaaacta caaccgcctg ctggacgcca agccggaaga gcggccggcg ttggagcggg
     7921 ccttcaagga agacctaaaa cgccgcattg acgaaaccgc caaatacatc cggccaaaca
     7981 agggcacact ggaccaggca ttgatgttca ttcccagcga ggcgatttat tacgacctgc
     8041 tggccaataa agtcggcgcc gccggcgtgt ccggccgcaa cttgatgcag tacgccgcca
     8101 aagataaaaa ggtcacgatt gtcgggccaa gcacgctttc cgccatgctg caggttatcg
     8161 tccagggcct gcgcagcata gaaatccaaa aagacaccga caaaatccgc aaaaacattg
     8221 agcagctcag caaacacctg gtcgcccaca acggctacat gcaaaagctc ggcgcctcac
     8281 tcggcacaac ggtcggtcat tacaatgcga cttataaaga actgggcaaa attgataaag
     8341 acgtggttaa aatcgccgat accgaaccgt ccgtcgagcc aattcttatc gatcggccga
     8401 accttgacga ctaagctaca tttccgtttg gcataattcg accacgtgag tacataaacg
     8461 cgccaaacta tcataaacgg ggattttgtg ttccttagcg caacttttta tatagtcttc
     8521 attacctcct gtgtagtcag ggtcaatacc gatcccaagc ctaattaaat atgcgtagtc
     8581 tttccaacca atagctcgcc caagctcaat tcttgaagtt tgagcatagg ctctgcccac
     8641 cgggtaatct ttgattgaat gatcttgggc ggcaaaccaa aacataatag ctccgtatct
     8701 tcgagcatgg agaaggtact tcttttccca caatatctga tcttcttttg tatgatccag
     8761 gctgctcgct cttgggttaa aaatgttcac agtggcttca ttttttagta aatcctcaaa
     8821 atgatttact gcgtcagctt gccaatttgg agcgccttgt atcggtccgg ctaaaaatat
     8881 acctggacca tcactgttct gaatctctgg gggccgatga actctaattg acataaaata
     8941 agcaaatcat cccggcttaa cttatgcaag cactaggatt ttaagaggtc gatgatgagg
     9001 gcggcagtcc aggagaagtt ttgggcgccg gccggctcgg ccgtcaaggg gttgaagtac
     9061 tcgtaaaagc cgcttttagc tactaagtcc aagctctttt ggcgcaaaga cgcggcttct
     9121 ttgctgaaac cataattttc tagcccttta atgatcagcc agtcggtgtt tatccaagcc
     9181 ggaccttgcc agtagcggca aggctcaaag taaggggaat ttcgcggcac gctgggcacc
     9241 ggccaattgc tggcgaacca ttttttatct tgcaaaagtt ccaccaagcg ggccgcgcgt
     9301 tccttactga tacttccggc gtacagcggc agcagcgtgg ccacagatga ttcttctatc
     9361 agtttatggc tgacaaaact gcggctgaaa tattgcccgc tggattcatc ccaaagctgt
     9421 tccagcgccg attctgtgtt ggccatgttt tttaataacg ccggcggaag ttcttgcttg
     9481 atatcagcgg cgattttctt taagcaatcg ttggctcgga tgaaaataca gttgaatgcc
     9541 aggtcttcca ggctgaaaag cgagcgcgat aagatagcct cggaattata agctttgcgg
     9601 cgcaggcggt gcatcgccgc ccagtaagca gcggcttcga tattgctcat gcgctgggcg
     9661 agtgtggtgt gctgggtgtc gcggcggaag aactgtatga gtgatccaag ccgggccttc
     9721 tcggcggcgg ttaaccacag aggcatattg tgtttacgaa gttcgcttat ccagggcggg
     9781 gaatcgtcca agccgccttc atagggatga agtaaaacta tcaagccttc gtggtgcggg
     9841 tcgcgttcgt tatacagcca ttcgtggtag tgcagcaggg ccggatacat ctgttggtac
     9901 cagctgcggc gttccggcaa cttcaacttt tggcctatct gcagcacggc ttcggccagc
     9961 attggcggct gggtgatgcc gctggtggcg acttggctcg gcgaatacgg gctgacccag
    10021 ctgcgccata gctcgcggtc ccgccggtat tgtttggcgc cagaaaaaat gatgttgggc
    10081 agcatgccat tgctccactg gccgcgcagc aggctcttta gttcggtttg cgcccgctct
    10141 acgttcaaat gccgcaggcc gatggcaata aaacaactgt cccataacca ctggtgagga
    10201 tataaattgg ctgccggcat aacccacttg ccgcggtcat tgtgctttaa gactttttcc
    10261 gcctcttgca atagcctggt ttgctcctca gccataagct taagtatata acaagggtat
    10321 aagggatagg gcataggggt tagtgttgat atactaaata gcgaccatgg agtacaagaa
    10381 ctcagagatt gctaaagctg ccaagcagct gaaagataag ttagccaagc ttgatgacaa
    10441 acgggcgatt ctgcgggcgc cggagctggc ggctttgtac gagcggctca aaactctgcc
    10501 agccggcaag ccgcgcgccg acttcggtaa gcaagtcaac gcgctgcgca atgaactaga
    10561 acaattaatc cgcgactcac tacccaccac ccgccaccct ctacccccta ttgacgtcac
    10621 ggcgccgttt gatgccaatg tcaaacccga agaccggccg gatctgctgc cggccaaaag
    10681 tggcagcatc cacccgatta acacagaact caagatcatt ctagacattt ttggccgaat
    10741 gggttttgaa gtggttgaat cacgcgagat agacgacgat taccatatgt tcggcagctt
    10801 gaacttccca cccgggcacc cggcgcgcga cgaatacgac acttttgtaa ccacggaagg
    10861 gctgatcgcg ccagcgcaca ccagcatcat gcaaaaccgg gtgctaaaaa cccgcaaacc
    10921 gcccatccgc gccgtaattc ccggccgagt tttccgcaat gaagacttgg acgcccgtca
    10981 cgaacacact ttttaccagc tggagggcgt ttacgttgat gaaggcatca acgccggcca
    11041 cttggttgcc accatccagg cctggcttag caattacttt gagcgagacg tgaacatcaa
    11101 aatccagcct ttctacttcc cattcaccga acccagtttt gaattcgccg ccagctgccc
    11161 gttctgcgcc ggcaagggct gcagcgtttg cagccacacc ggctggatag aacttggcgg
    11221 ctgcggcatg atccacccca acgtcttgaa agaaggcggc gtggacgcca aaaaatacac
    11281 cggctttgcc tggggcttcg gcattgagcg cctagtgatg ctcaaataca acattgaaga
    11341 tgtccgtctc tttgaatccg ccaacctaaa attcctgagg caattcgaat gaaaaatgcg
    11401 attctaatcc acggctggaa taccaaagaa gaatattacg acccagttcg tccaacagct
    11461 agtaatgatc attggttccc ttggctgaca aaacaactgg tgctaaaaga catcaatacg
    11521 gtgtcggttg aaatgccaga tggctactac cccgaatacc atatttggaa acgcgaactg
    11581 gagcgttatg acatatcgag caagacaacg ttagtaggcc atagttgtgg cggcggattt
    11641 ttggtgaggt ggttaagcga aaacgacgtt aaagtcggta aagttatatt agttgccccc
    11701 tggcttgggc tagataccca gggcgagcca tttgataaaa gtttttttga ttttgaaatc
    11761 gatccaaaaa ttgctgaaaa aacggccggt ttgagcattc taatatctga tgatgactac
    11821 agggacgtgc ttgaaagtgt cgaaatattg cgtaaaaaat tagacggggt gaacattaaa
    11881 gagtttcacg gcaagggaca ctttgtgctc agttctctcg gcggcccaga atttcctgaa
    11941 cttctcgagg agatcctaaa atgaaggtaa gcttgaattg ggttaaggaa tttacctcgg
    12001 ttgatttgcc ggtggatgag ctggtggaaa aaattggcgc gcaattgggc gccgttgagg
    12061 aagtagaaga aatcggcaaa aaataccagg gcattttggt ggtcaaagtc gtcttggcgg
    12121 aaaaacaccc gaatgccgac agactaacct tttgtttagt agatgatggc aaagccaaca
    12181 aaaaggtcaa acgcaataag gatggcctaa tagaaattgt ttgcggcgcg ccgaatgtaa
    12241 agaccggcca gctggcagtt tggatcccac ctggagcgac cgtacctgca acgtatgata
    12301 aagagccatt tgttattgaa gctcgggaga taagaggtaa gaccagccat gggatgctag
    12361 ccagccttaa ggaattggca ttgggcgata gtcacaacgg aatcgtagtg cttgacatgg
    12421 gtaaaccggg cgacagcttt gccaagacgc tctggctgga cgactacatc attgatatag
    12481 aaaataaaat gtttactcac cggccggatt gtttcggcat gcttggagtg gcccgcgaga
    12541 tagccggtat ccagcacaaa gctttccaaa gcccggactg gtaccgtgaa aacgccagct
    12601 tgccgaccga cgggcgcaaa aacgtcttga aaataacagt caaaaatgac gttccgaaac
    12661 tggtgccgcg tttttgcgcc gtagccatca aagacgtcaa ggtggaaccc agtccagctt
    12721 ggatgcagag cttcttggcg cgcgtcggcg tgcggccgat taataacatc gttgatatca
    12781 ctaatttggt gatgctggag accgcccagc cgatccatgc gtacgattat gacaagctca
    12841 agaccggcgt cctgggcgtc cgtttatcca agaagggcga gcaacttaag ctgctgggtg
    12901 gcaaaaccat taaattggac gacggtgcag tggtgataac cgacggcaag cagcccgtcg
    12961 gcctgggcgg cgtcatgggc ggcgccggca ccgaagttga tgaaaacacc aaaaacatcg
    13021 tcctggaagt agcaaatttt gacatgaacc tgacccgccg cacggctatg acctatggct
    13081 tatttacaga cgcagccgtc cgcttcacca aaggccaatc gcctgatcag aatatggcgg
    13141 tgctgtgccg ggcggtgaat catgccctgc aatttgccgg cggccgccag gctagcgcgg
    13201 tcgtagacga cttcgcgaaa caagaaacaa gaaacaagaa acaagagatt gttgttggtg
    13261 cagaattcgt gaacagccgg ttaggcctga atttgagcgg cgccgaaatc aaaaaactac
    13321 tggagaacgt tgaatttaaa gttgaattga gcggtggctc gctcaaaatt actccgccat
    13381 tctggcgcat ggacattgcc attccggaag atatcgttga agaagtcggc cgcctttacg
    13441 gctacgataa actgccggta gtcctgccgc cgcgcgacct gtcgcctgtc cagcctaacc
    13501 cgagagtggt tttcaaagac cgtttgcgtg atattttaag tcgtgctggc gccaacgaag
    13561 tgctgaccta cagctttgtg cacggcagct taatgcaaaa agtcggccag gacccgaagg
    13621 acgcctaccg gatcgccaac gccctcagcc ccgatgtgca gtattatcgc ctcagcctca
    13681 cgccc
//
LOCUS       DASPTF010000079         5800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_561618, whole genome shotgun sequence.
ACCESSION   DASPTF010000079 DASPTF010000000
VERSION     DASPTF010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561618"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VEH48_02700"
     CDS             complement(<1..430)
                     /locus_tag="VEH48_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G1 family glutamic endopeptidase"
                     /protein_id="HXY18302.1"
                     /translation="MYARIRTNVKGVAAKLRYFFVICVLLGMLITTLFTPVVSAQAAA
                     VKPASIGSITAGPGRRFLNSNASILRSSLAKRFTNNASGSNSPTIYYNWSGYAATSTS
                     AFNEVQSTFVQPAVTCPVPGAWTLFWVGFDGFTNNTVEQAG"
     gene            complement(606..1049)
                     /locus_tag="VEH48_02705"
     CDS             complement(606..1049)
                     /locus_tag="VEH48_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXY18303.1"
                     /translation="MDQAQAYQSQKPQKIVIVEDTVDLAEIYKTRMEALGYQCIVAND
                     GISALYLIQKELPDLVLLDLMVPNIAGDQILARMRASEWGKKIPVVIISNLNEADAPP
                     GIRNLGIAGYAVKANLSYDQLDQLVNSLLRPGAQNENPEQNQNPQ"
     gene            1170..1961
                     /locus_tag="VEH48_02710"
     CDS             1170..1961
                     /locus_tag="VEH48_02710"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXY18304.1"
                     /translation="MKNIQELLDLKDKAAIVTGGAKGIGYGISYRLAEAGAKVMIADL
                     DEAVAQGATQELTNKGWSAEAFRADVSSEQDVQQMINACKEKFGSLDILVNNAGIYPS
                     VPLAQMTEDEFEKVMHVNLRSVFLTTKYAAEVMKQQGGGKIINITSIDALHPSMAGLA
                     HYDASKHGVWGFTKNSALELAEHKIWVNAVAPGGIATPGSAAASQGASAEQMAAQTEA
                     FMAKIPMHRMGEPDEIGMVVLFLASDMSSYMTGEQIVVDGGALLS"
     gene            complement(1958..4330)
                     /gene="ppsA"
                     /locus_tag="VEH48_02715"
     CDS             complement(1958..4330)
                     /gene="ppsA"
                     /locus_tag="VEH48_02715"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HXY18305.1"
                     /translation="MYKHVKFFKDISLADVPEVGGKNASLGEMYKYLAPKGINLPNGF
                     ATTAKSYYYFLDQAGIRDEIENILQGLDVKNVKDLEKRGARIRSLIVRARLPKDFEEE
                     IRQGYRELSQACGSRNLVVAIRSSATAEDLPNASFAGQQATYLNIKGERHVLRAAKEC
                     ISSLFTDRAIVYRVENNFAHMKVALSVGVQQMVAVRSKAAGIMFTIDTESGFRNAVIV
                     SSIYGLGENIVQGRVSPDEFTVFKPTLAILKRKLGTKKEKMIPISRSRTKNVAVSKPD
                     QACYSISDEQVKTLAKWAMEIEKHYGKPMDIEWALDQDDGKLYIVQARSETVQSRKNM
                     NVIEEYRLTDSGTPVVKGTSVGNKIGKGQVSKIMSTRDINLFKAGDVLVTEMTDPDWV
                     PIMKIASAIVTDKGGRTCHAAIVSRELGIPCVVGTGNATQKIADKSFVTVSCTEGDEG
                     YVYPGDLKFEIKRTDIKNLKRPKTRIMMNIGSPDHAFAYSMIPNDGVGLAREEFIIDS
                     HIKIHPLALLRFNEVTDKKARAQISDLTAGYSDKAQYFIDKLAEGIGIIGAAFYPNDV
                     IIRFSDFKTNEYAGLIGGAQFEPKEDNPMIGWRGASRYYSDGYRDAFELECKAFKKAR
                     NELGLTNIKAMVPFCRDLTEAKKVLDIMEKHGLKRGENGFETYVMIEIPSNVILAEQF
                     AELFDGFSIGSNDLTQLTLGVDRDNHTVAHIYDENNAAVEELIRRVIKVAKAKHVKIG
                     LCGQAPSDYPQFARFLVEAGIDSISLSPDTVVRTTIDVKKTEDKLRRARS"
     gene            complement(4426..>5800)
                     /locus_tag="VEH48_02720"
     CDS             complement(4426..>5800)
                     /locus_tag="VEH48_02720"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HXY18306.1"
                     /translation="TYGEATVAEYAFTLLLALQRKLPEVLEAEKSQFSSDELIGHDLE
                     GKVFGVIGTGHIGQKALKIADGFSMKTIAFDSYPKNELQDEYHFQYVGLDELLEQADI
                     VSLHVPYLPSTHHLMNRDRLSKMKNGAILINTARGELVDTNALTELLDSGQLGGAAID
                     VIEGEALMNYREETALLRSDTLPEDVLRHSVEISVLEKMPNVIISPHNAFNTVEAIHR
                     INQTTAKNIIDFWYGNVPNEVTAAKKPFGKLLVARHAESEWNASGKWTGITDVHLSEK
                     GFRESAMFGQALKKLDIPINVAYCSEQIRTRETLEGMLDAAQQFDVDIVTNSGLNERD
                     YGEYTGKNKWEMKELIGEEAFNNLRRGWDVPIPGGETLKMVYERVLPFYTGTVLPLLR
                     QGKNVLIVAHGNSIRALVKYLESISDEDVCKLEMSFGEIIIYNVSEDGRRAGSSVVKI
                     DTTPPDA"
BASE COUNT         1234 a         1501 c         1572 g         1493 t
ORIGIN      
        1 taccagcttg ctcaacggtg ttattagtga aaccgtcaaa accgacccag aacagcgtcc
       61 aggctcccgg caccggacag gtgacggccg gctggacaaa agttgattgt acctcgttaa
      121 atgcgctggt tgacgttgcc gcgtaaccgg accagttata atatatcgtc ggactattgg
      181 aaccgctggc attgttagta aagcgtttag ccagcgaaga acgcagtatt gaagcattgg
      241 agttaagaaa tcgccggccc ggaccagctg ttatggaccc gatggaagcc ggtttaacag
      301 cggccgcctg ggcggacaca acaggggtaa ataaagtggt tatcagcatg cccaatagta
      361 cgcatatcac aaaaaaataa cgcagtttgg ctgcaacgcc cttaacatta gtccgaattc
      421 ttgcgtacat cctcaaaatc ctttgtctac aatcactaat agatcattaa ttacggttga
      481 tattatacac tagtttatgc attactgtca atagtatact atcgtatagt aattcaaaga
      541 gttgtggctg aaatggcttt caatatttat tgtaagaaaa tcgagaatta agagctaagc
      601 ccgttttact gggggttttg attttgctcc ggattttcat tttgggcgcc gggcctcagc
      661 aggctgttca ccagctggtc gagttggtcg tatgataagt tagccttgac ggcgtagccg
      721 gctatgccca aattgcggat ccctggcggc gcgtcggctt cgttcagatt ggaaataatc
      781 actaccggta tctttttgcc ccactcactt gcccgcatgc gcgccaaaat ctggtcgccg
      841 gcaatattcg gtaccatcag gtccagcagc accaaatccg gcagctcttt ttgaatgagg
      901 taaagcgccg agatgccgtc gttggccacg atgcactggt atccgagtgc ctccatcctg
      961 gttttgtaga tttcggccaa atctaccgta tcttcaacaa tcacgatttt ttgcggcttc
     1021 tggctttgat acgcttgtgc ttggtccata gtgttgattt tagcgcacaa cggccgtctt
     1081 ttctagcagc ggactttgat ttatcaagaa gttggatgat tcggggcgcg ccgcggcaaa
     1141 taccgagtag aataaaagta ggagaaaata tgaaaaacat acaagaatta ttggatttaa
     1201 aagataaggc cgcgatagtg accggcggcg ccaaaggcat tggctacggc ataagctacc
     1261 gtttggccga ggccggggcc aaagttatga ttgccgatct ggacgaagcg gttgcccaag
     1321 gggccacgca agaactgacg aacaaaggct ggtctgccga agccttcagg gccgacgttt
     1381 ccagcgagca agacgtccaa cagatgatta acgcctgcaa agaaaaattt ggctcgctgg
     1441 acatcctggt gaacaacgcc ggtatttatc catccgttcc gctggcacag atgacagagg
     1501 acgagttcga gaaagtcatg cacgtcaacc tgcgcagcgt attcctgact accaaatacg
     1561 ccgctgaagt tatgaaacag cagggcggcg gcaagatcat caacatcact tcaattgacg
     1621 ccctgcaccc ctccatggca ggtctggccc actacgatgc ttccaagcac ggcgtctggg
     1681 gctttaccaa gaattcggcg ctggagctag ccgaacataa gatctgggtc aacgccgtcg
     1741 ccccgggcgg tatcgccacg cccggctcgg cggctgccag ccaaggtgct tcggctgagc
     1801 agatggcggc ccaaacagag gcatttatgg ccaaaatccc gatgcaccgc atgggcgagc
     1861 ctgacgaaat cggcatggta gttctgttct tagccagcga catgtccagc tacatgacag
     1921 gcgaacagat agtggttgac ggcggagctt tgctgtctta agatcgggcc ctgcgcagtt
     1981 tatcttcggt ttttttaacg tcgatggtgg ttctgacgac ggtgtccggg gagagggaaa
     2041 tgctgtctat gccggcttca accaggaaac gcgcgaactg gggatagtct gacggcgcct
     2101 ggccacagag gccgatcttt acgtgtttgg ctttggcgac tttgattacc cgccggatga
     2161 gctcctcgac cgccgcgtta ttttcgtcat aaatatgagc cacggtgtgg ttgtcccggt
     2221 caactcccaa agtcagctgg gtcaggtcat tggagccgat agaaaagccg tcaaatagct
     2281 cggcgaactg ctctgccaaa atgacgtttg acgggatctc aatcatgacg taagtctcaa
     2341 agccgttttc gccgcgcttc agtccatgct tttccatgat gtccagaact tttttagcct
     2401 cggtcagatc gcggcagaac ggcaccatgg ctttgatgtt tgtcaggccc agttcgttgc
     2461 gggctttttt gaaggctttg cattccagct cgaaggcgtc gcggtagcca tcggaatagt
     2521 agcggctggc gccccgccag ccgatcatcg gattgtcttc tttgggctca aactgggcgc
     2581 cgccgataag accggcgtat tcattggttt taaagtcgct gaagcggatg atgacgtcgt
     2641 tggggtaaaa agccgcgcca atgatgccga tgccctccgc cagcttatca ataaagtact
     2701 gggctttgtc gctgtagccg gccgtcaggt cggaaatctg cgcccgggct tttttatctg
     2761 ttacttcgtt gaagcgcagc agtgccagcg ggtgaatttt gatgtgcgaa tcaatgatga
     2821 actcttctcg ggccagcccg acgccgtcgt tgggaatcat tgaataggcg aaggcgtggt
     2881 ccggcgagcc gatgttcatc ataatccttg ttttgggccg cttgaggttt ttgatatctg
     2941 ttcgtttgat ctcaaacttc agatcgccgg ggtaaacata accttcatcg ccttcggtgc
     3001 agctgacagt aacgaaactt ttgtccgcga ttttttgcgt ggcgttgccg gtaccaacta
     3061 cgcaggggat accgagctcg cgcgaaacga tggcggcgtg acaggtacgg ccgcccttgt
     3121 cggtgacaat ggcgctggct atcttcatga tcggcaccca atccgggtcg gtcatctccg
     3181 ttaccagcac atcgccagct ttgaacagat tgatgtccct ggtgctcatg attttgctga
     3241 cctggccctt gccaatctta ttaccgacgc tggtaccctt gacaacagga gttccgctat
     3301 cggtcagccg gtattcctct atgacgttca tgttcttgcg cgactgcacg gtttcggagc
     3361 gggcctgcac gatgtacagc ttgccatcgt cctggtcaag cgcccactca atgtccatgg
     3421 gtttgccgta gtgcttctca atctccatgg cccacttggc cagggtcttt acctgttcat
     3481 cgctgataga gtaacatgcc tggtcaggtt tggaaacagc gacgtttttg gtccggctgc
     3541 gggagatcgg gatcattttt tctttcttgg tgccaagttt ccgcttaagg atcgccagcg
     3601 tcggcttaaa gactgtgaac tcatcgggac tgacgcgccc ctggacgata ttttcgccca
     3661 gcccatagat tgagctgacg atcaccgcgt ttctaaagcc cgattcggta tctatggtga
     3721 acatgatacc ggcggccttt gagcgcaccg ccaccatctg ctggacgccg acggacagcg
     3781 cgactttcat gtgcgcgaag ttattttcca cgcggtagac tatcgcgcgg tcggtaaaaa
     3841 gcgatgagat acattctttg gcggcccgca ggacgtgtct ttcgccctta atgttcagat
     3901 acgtcgcctg ctggccggcg aaggaagcgt ttggcaggtc ctcggccgtg gccgatgaac
     3961 ggatggctac caccaggttg cggctaccgc aggcctggct tagctcgcgg tagccttggc
     4021 ggatctcctc ttcaaaatct ttcggcagtc tggccctgac aatcagcgag cggatccggg
     4081 cgccgcgttt ttccagatct tttacgtttt tgacatctag gccctgcagg atgttttcaa
     4141 tttcatcgcg gatgcccgcc tggtccagga aataataata acttttggcc gtggtggcga
     4201 aaccgtttgg caggttgata cccttgggcg ccaggtattt atacatttcg ccgagcgaag
     4261 cgtttttgcc gccgacctct ggaacatcgg ccagcgatat gtctttgaaa aactttacgt
     4321 gtttatacat cttgatttac ccgcttctat ggctttttat ttgattgccc ttcagattga
     4381 attataacac tttttaccgt tagcggtttt atcgaccctc ttttattaag catcaggcgg
     4441 cgtagtatct atttttacga ccgatgaacc ggcgcgccgg ccgtcctctg agacgttgta
     4501 aatgatgatc tcgccaaaag acatttccag cttacaaaca tcttcatcgc tgatagattc
     4561 caggtatttt acgagcgccc ttatgctgtt accgtgagcg acgattaaaa cgtttttgcc
     4621 ctggcgcagc agcggcagga cggtcccggt gtaaaatggc aggacgcgtt cgtaaaccat
     4681 cttcagggtc tcgccgccgg ggatcggcac gtcccagccg cggcggaggt tattaaaagc
     4741 ctcttcgccg ataagttctt tcatctccca cttatttttg ccggtgtatt cgccgtaatc
     4801 ccgttcgttc aagccgctgt tggtcacgat gtctacgtca aattgctggg ccgcgtccag
     4861 catgccttcc agcgtctcgc gggtgcggat ctgttctgag cagtaggcga cattgatagg
     4921 gatgtccagt tttttaaggg cctgaccgaa catcgccgat tccctgaagc ccttttcact
     4981 cagatggaca tcggtgatgc cggtccattt gccgctggcg ttccactccg attcggcgtg
     5041 acgggccact aataattttc cgaagggctt tttggcggca gtaacttcgt taggtacatt
     5101 tccataccag aaatcaatga tatttttcgc ggttgtttgg ttgatgcggt ggatggcctc
     5161 gacggtgttg aaagcgttgt gggggctgat tatgacgttc ggcatttttt ccagcaccga
     5221 tatctcgaca ctgtggcgca agacgtcttc gggcagagtg tcgctgcgca gaagagctgt
     5281 ctcttcgcgg tagttcatca gggcttcacc ctcgatgaca tcgatggcgg ctccgccgag
     5341 ctggccgctg tccagcagct cggtcagtgc gttggtatcg accagctcgc cgcgggcggt
     5401 attgatcaag atggccccgt tcttcatctt actgagccgg tcgcggttca tcaggtgatg
     5461 ggtcgagggc aggtagggca cgtgcaagga cactatatcg gcttgctcca gcagttcgtc
     5521 tagcccgaca tactggaagt gatattcgtc ttgaagttcg ttctttggat agctgtcgaa
     5581 ggcgatggtt ttcattgaga agccgtcggc gattttcaga gccttctgcc cgatgtggcc
     5641 ggtgcctatc acgccgaaga ccttgccctc caggtcgtga ccgatcaact catcagatga
     5701 gaattggctt ttttcggctt caagcacttc gggcagttta cgctgcaggg ccagcagcag
     5761 cgtaaaggcg tactcggcga ccgtggcctc gccgtaggtc
//
LOCUS       DASPTF010000080         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1573080, whole genome shotgun sequence.
ACCESSION   DASPTF010000080 DASPTF010000000
VERSION     DASPTF010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573080"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VEH48_02725"
     CDS             complement(<1..412)
                     /locus_tag="VEH48_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HXY18307.1"
                     /translation="MTFYFYDLETSGFSPREARIMQFAGQRTDMNLKPVEKPDNILVK
                     MTPDVLPQPDAVLVHGITPQQTIAEGISEAEFAKYLTSQVFTPDTIAIGFNNIRFDDE
                     FVRFTFWRNFMDAYEWAWKDSRSRWDLLDVARMTR"
     gene            complement(414..1688)
                     /gene="obgE"
                     /locus_tag="VEH48_02730"
     CDS             complement(414..1688)
                     /gene="obgE"
                     /locus_tag="VEH48_02730"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXY18308.1"
                     /translation="MFVDRVRVRVEAGNGGDGALSFRHEKFRARGGPDGGDGGHGGSV
                     ILRASRNQNTLAAFRYKKLLKAEDGQPGGKRKRHGKSGADLEVAVPVGTAVSVDEKIL
                     ADLTTDGQEVTIARGGKGGFGNAHFVSSTRQAPQVTEKGEKGQQLEAGFELKMIADVG
                     IVGLPNAGKSTLLSVISNARPEIADYPFTTLTPNLGVVDIDKKHSLLFADIPGLIEGA
                     SQGKGLGDEFLRHVERTSVLVHLIDAYSEDVAEAYKTIMNELAAYKVDLSNRPQVVAL
                     TKIDGLDKKQLGKAYKTLKSTVPRSTATAAISANSGEGVAALLRTLRKLVEKNRKAAG
                     GKAEKAPLPVIGLKEDDDAWQIRQEKDTFIITGRKIERFAARTRFGDYHAEQRLRDIM
                     YKMGIIRELERRGIEPGQTISIGQPEIGSLNY"
     gene            complement(1730..2890)
                     /locus_tag="VEH48_02735"
     CDS             complement(1730..2890)
                     /locus_tag="VEH48_02735"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HXY18309.1"
                     /translation="MHLIIRKPNLSFLKRKSTRGLEAIMALPDLTGRINKLDKKSRRR
                     VIRWGIIGGNCLLLLVIAVFLLVNRSTSQTVRSSTADSAVGTASSVSDPLDQLSSDQI
                     ALQAAQMTELPELTMVRNRADSTAILLSLVPNDSTTLAKPQVVSTAEKSRYDIIHYTV
                     AAGDSVGSLATRFGVSANAIRWSNGLSDDTLNAGAVLLIPPAEGIVYQVKNGDTVATI
                     VNKYQANLDTFITVNDAENGITTGELVWIPNGIQPVLVTALNLQSISAGSFSGAHRFG
                     SCSTGINNGYDCGWCTWWAAFRRSQIGNPIPPNWGDAYTWAAAAAEMGHVVSFTPVVG
                     AIIWFPYDHVGFVESVNSDGSITISEMNQEGWDVVDYRTIPAGQVGNYKYIY"
BASE COUNT          584 a          815 c          820 g          684 t
ORIGIN      
        1 cccgggtcat tcgtgccacg tccagcagat cccagcggct gcgactgtct ttccaggccc
       61 attcgtaggc gtccatgaag ttacgccaga acgtgaagcg cacgaactcg tcgtcaaaac
      121 ggatgttatt gaaaccgata gcgatcgtat ccggcgtaaa tacttgacta gtcaagtatt
      181 ttgcaaattc agcttcgcta atgccttcag ctatggtctg ctgcggcgtg atgccgtgga
      241 ccagcacggc gtccggctgc ggcagaacgt ccggcgtcat ctttaccaaa atgttatccg
      301 gcttctccac cggcttgagg ttcatgtccg tccgctggcc ggcaaactgc ataattcgcg
      361 cctcgcgcgg actgaagccg ctagtctcca ggtcgtagaa ataaaacgtc atatcaatag
      421 tttaggctac cgatttccgg ctggccaatg cttatggttt ggcctggttc tatgccccgg
      481 cgctcaagtt cgcggatgat gcccattttg tacatgatat cgcgcaggcg ctgctcggcg
      541 tgataatcgc caaagcgggt gcgggcggca aagcgctcga ttttgcggcc ggtaattata
      601 aaggtgtctt tttcctgccg gatttgccag gcgtcatcat cttctttgag cccaattacc
      661 ggcagcggtg ccttttcagc tttgccgccc gcggccttcc ggttcttttc caccagtttc
      721 cgcagagtcc gcagcagcgc cgccaccccc tcgccgctat tggctgatat ggctgcggtg
      781 gcggtgctgc gcggaaccgt gctctttaac gttttataag ctttgcccaa ttgctttttg
      841 tccaggccgt ctattttagt cagggccacc acctgcgggc gattactaag gtcaactttg
      901 taagccgcca gttcgttcat aatggttttg taagcttcgg caacatcttc gctataggcg
      961 tctataaggt gcaccagcac gctcgtgcgc tccacgtggc gcaggaattc atcgcccaga
     1021 cccttgccct ggctggcgcc ttctatcagg cctgggatat cagcgaacaa caggctgtgt
     1081 tttttatcta tgtctaccac gcctaaattc ggcgtcagcg tagtgaacgg gtaatcggca
     1141 atctccggcc tggcgttgct gataacggac aaaagagtgg atttgccggc gttgggcaaa
     1201 ccgacgatgc caacgtcggc gatcatctta agctcaaagc cggcttccag ctgctggcct
     1261 ttttcgcctt tttcggtcac ctgcggcgcc tggcgagtgg agctgacaaa gtgggcattg
     1321 ccaaaaccgc ctttgccgcc gcgggcaata gtcacttcct gaccatcggt tgttaggtca
     1381 gccaaaatct tctcgtccac cgaaaccgcc gtgccgacag gcacggccac ttccaaatcg
     1441 gcgccgcttt taccgtggcg cttgcgtttg ccgcccggtt ggccatcttc ggccttgagc
     1501 agttttttat aacggaaagc ggccaaggta ttctggttgc ggctggcccg caaaattacg
     1561 ctgccgccgt ggccgccgtc accgccgtcc gggccgccgc gggcgcggaa tttctcgtgg
     1621 cggaaactca aagcgccatc accgccgttg ccggcctcaa ctcttacccg cactctatct
     1681 acaaacatac ataagatagg gtatagggtt tagggactag ggtaaagggt taatagatgt
     1741 atttgtagtt gccgacctgg ccggcaggaa tagtgcggta atccaccacg tcccagcctt
     1801 cctggttcat ctccgatata gtgatgctgc cgtcagagtt gacactttcc acaaagccga
     1861 cgtggtcgta agggaaccag atgatcgcgc caacaacggg cgtaaatgat acgacgtgcc
     1921 ccatctctgc agcggcggcg gcccaagtgt aagcgtcgcc ccagttgggt ggtatcgggt
     1981 taccgatttg tgagcggcgg aaagccgccc accaggtgca ccagccgcag tcgtagccgt
     2041 tgttgatacc ggttgagcac gagccaaaac ggtgcgcgcc gctgaaactg ccagcggaaa
     2101 tcgactggag attcagggcg gtcaccaaca ctggttgaat gccgttggga atccagacca
     2161 attcaccggt tgtaatgccg ttttcggcgt cattgacggt tatgaacgta tccagattgg
     2221 cctggtactt attgacgatg gtggccacgg tgtcgccgtt tttgacttgg taaacgatgc
     2281 cctcggccgg cggtatcaat agaacggcgc cggcattcaa ggtgtcgtcg ctcaagccgt
     2341 tagaccagcg gatggcgttg gcgctgacac cgaacctggt cgccaggctg ccaacgctgt
     2401 caccggcagc aacggtgtag tggatgatgt catagcgcga tttttcggcg gtggagacca
     2461 cttgtggttt ggccagagtc gtggaatcgt tcggtaccag cgacaacaag atggctgtag
     2521 agtcggcgcg gttgcggacc atagtcagtt ctggaagttc agtcatctgg gcggcctgca
     2581 gagcaatttg gtcggaagac agctggtcca gcggatcgct gaccgagctg gcggtgccca
     2641 ccgcgctatc ggcggtgctg ctgcgcaccg tctggctggt tgagcggtta accagcaaaa
     2701 aaaccgcgat gaccagcaat agtagacaat taccgccgat aatcccccag cggattaccc
     2761 ggcggcggga tttcttgtcc agtttattga ttctgccggt cagatccggc agagccatga
     2821 tagcttccag gccgcgcgtg cttttacgct taaggaacga caggttgggt ttacgaatga
     2881 ttaaatgcac tccttaaaat aac
//
LOCUS       DASPTF010000081         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_849912, whole genome shotgun sequence.
ACCESSION   DASPTF010000081 DASPTF010000000
VERSION     DASPTF010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849912"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VEH48_02740"
     CDS             complement(<1..1118)
                     /locus_tag="VEH48_02740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXY18310.1"
                     /translation="MVKSAEKLSVGFLFDDTLDSSDGVAQYVKTLGAWLSSQGHQVCY
                     LVGETKIKSWADGRVYSLAKNQRVVFNGNRLSIPLPARRDRIKQVLRENKFDVLHVQV
                     PYSPFMAKKVIDAADASTAVVGTFHILPANGLARAGSRALRLLYGKSQRRFDAMLSVS
                     APAAEFARRVYGIRSQVLPNVVDINRFQKTQERRSGKPTIVFLGRLVERKGCRQLLKA
                     FKQLNSRLPGARLIIAGDGPQRRSLENYVRRNGLCQQVKFLGFIDEEAKPGLLAGADI
                     ACFPSLYGEAFGIVLIEAMAAGSGVVLGGDNSGYRSVLGDQPQLLIDPNDTEVFARRL
                     ESLLSDRARIEGLHSWQQAEVRQYDINAVGKRLVDIYR"
     gene            complement(1060..2322)
                     /locus_tag="VEH48_02745"
     CDS             complement(1060..2322)
                     /locus_tag="VEH48_02745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXY18311.1"
                     /translation="MKIALVCPYNMLDRPGGVPQVVMHLHEGLKKKGHVVKVITQRPS
                     SFEGQAPEDYILFGITRTFKISGFGTEGNWGMPADGEEIAKILEQEQFDVINFHEPWL
                     PMLAWQMLKHSKAAHVGTFHANLVDTAAGKSWTSSIFTPYGRPLLKKMHLFTATSPAA
                     SGMLISRANMKSPHEKSLIENLKYIPCGVELSVYKPVKKRLPLNGPNTKTIVYVGRLE
                     KRKGVDWLLPAFAELQKEMPNVHLIIAGSGIRANRLREYVNMENIKNVAFPGYVTDEE
                     KRRLLCNADLACFPSSYGEGFGIVLLEAMAVGTPLVAGSNLGYKNVMTGHGRIGLVDP
                     EATADFANRLAVMLSDNDLRKLMVSWELADVKKYDYPKIVAQYEAAFKEAIILKNKSA
                     RSKSSAGNNNGKKRRKIIRRLFVRRHAG"
     gene            complement(2342..>2775)
                     /locus_tag="VEH48_02750"
     CDS             complement(2342..>2775)
                     /locus_tag="VEH48_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18312.1"
                     /translation="VAVVIRYIGLNKPVIKLLTNTLPNSKHRFIEVDAIEFDSSLDFE
                     VLLWQANDVRTHWLTTSEFLRLDNCRYPVKSPVVHIISAGDHYINNIKVEQHMRQIFS
                     SYKQFSAASKAHVPSVIADKKAMAVMVPAGLRRLLGKKGRS"
BASE COUNT          616 a          767 c          731 g          661 t
ORIGIN      
        1 tggcggtaga tatcaaccag tcgtttaccg acggcattta tgtcgtactg ccggacttcg
       61 gcttgctgcc agctgtgcag accctcaatc cgcgcccggt cgctgagcag tgactccagc
      121 cgccgggcaa aaacctcagt atcgttgggg tctataagca gctgcggctg gtcgccgagc
      181 acgctgcggt agccggaatt atcgccgccc agcaccacgc cggacccggc ggccatcgct
      241 tcaattagaa ctataccgaa agcctcaccg taaagtgatg gaaagcaggc gatatcggcg
      301 ccggccagca gaccgggttt agcctcttcg tcaatgaagc ccaggaattt tacctgttgg
      361 cacaggccat tgcgacgtac gtagttttcc aggctgcggc gctgcggacc gtcgccggca
      421 attatcaacc gcgcgccggg caggcgcgaa ttaagctgct tgaaggcttt taataactga
      481 cggcagccct tgcgttccac caagcggccc aaaaacacta tcgttggctt gccggaacgc
      541 cgctcttgag ttttttggaa gcgattaata tcaaccacgt taggcaaaac ctggctgcga
      601 atgccgtaaa cccggcgcgc gaattccgcg gccggcgcgc tgacgcttaa catggcgtca
      661 aaacgccgct ggctcttgcc ataaagcagt ctcaaagctc gggagcccgc acgggcaaga
      721 ccattggctg gcaaaatatg gaaagtcccg accactgccg tggaagcatc ggccgcgtca
      781 atgaccttct tagccatgaa cggcgaataa ggcacctgca cgtgcaaaac atcaaattta
      841 ttctcgcgca aaacctgctt tattcggtcc ctgcgggcgg gcaggggtat tgacaagcgg
      901 ttgccgttaa aaaccacccg ctggtttttg gccaggctgt agaccctacc gtcggcccaa
      961 ctcttgattt tggtctcgcc gactagatag caaacttggt gaccttgcga acttaaccaa
     1021 gctcccagcg ttttaacgta ttgggcgacg ccgtcgctgc tatccagcgt gtcgtcgaac
     1081 aaaaagccga cggataattt ttctgcgctt tttaccattg ttgtttcccg cagatgactt
     1141 tgatctggct gatttattct tcaaaatgat cgcctctttg aaggcggcct catactgcgc
     1201 gacaatcttc ggataatcgt actttttaac gtcggccagc tcccatgaca ccattagttt
     1261 tcttaaatcg ttgtccgaaa gcatcacggc cagacggttg gcgaaatcgg cagtggcctc
     1321 ggggtcgacc aggccgatgc ggccatggcc agtcatgacg ttcttgtagc ccagattgct
     1381 gcccgccacc agcggcgtac cgacggccat cgcctccagc agaactatac caaagccttc
     1441 tccgtaagat gacgggaaac aggccagatc ggcattgcac agcagccggc gcttttcttc
     1501 atccgtcaca tagccgggga aagccacatt tttgatgttt tccatgttca catattcacg
     1561 taagcggttg gcgcgtatgc ccgaaccggc aattatcaga tggacattgg gcatctcttt
     1621 ttgcagctcg gcgaaggctg gcagcaacca atccactcct ttgcgctttt ccagccggcc
     1681 cacatagaca atggttttgg tgttcgggcc gttcagcggc agacgctttt ttaccggttt
     1741 gtaaacgctc aactccacgc cacaggggat atatttgaga ttctctatca aggacttttc
     1801 gtgaggtgat ttcatgttcg cccggctgat cagcatgcct gaggcagccg gcgaggtagc
     1861 ggtaaatagg tgcatcttct ttagtagcgg ccgtccgtat ggcgtaaaaa tggagctggt
     1921 ccaggattta ccggcagcgg tatcgacgag attggcgtgg aaagtcccca cgtgggcggc
     1981 cttggagtgt ttcaacatct gccaggcgag catcggcagc cacggttcat gaaaattgat
     2041 aacgtcaaac tgctcctgtt ccagtatttt ggcgatctct tcgccgtcgg ccggcatacc
     2101 ccaattgccc tcggtgccga aaccactgat cttaaaagtc cgggtgatac cgaataatat
     2161 gtagtcttcc ggcgcttggc cttcaaagct ggagggccgc tgggttatga ccttcactac
     2221 gtgacccttc ttcttcaggc cctcgtgcag gtgcatgacc acttgcggca cgccgccggg
     2281 cctatccagc atgttgtacg gacaaactaa ggcgatcttc attacttaac catcattata
     2341 cttaagaccg gccttttttg cccagcagcc ggcgcagtcc cgcaggcacc atgacggcca
     2401 tagctttctt atcggcgata acacttggca catgggcctt tgacgcggcg gaaaattgct
     2461 tgtaagagga gaatatctgc cgcatgtgct gttcaacctt gatgttattt atatagtgat
     2521 cgccggccga aatgatgtgc accactggcg attttacagg gtagcggcag ttatccaaac
     2581 gcagaaattc gctggtagtc agccaatgtg tacggacgtc gttggcctgc cagagcagga
     2641 cctcaaagtc cagactgctg tcaaattcta tggcgtcaac ttctatgaag cggtgttttg
     2701 aattgggcaa agtattggta agcagtttta taacgggctt attgagtccg atgtaacgga
     2761 tgactacagc gaccg
//
LOCUS       DASPTF010000082         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_131554, whole genome shotgun sequence.
ACCESSION   DASPTF010000082 DASPTF010000000
VERSION     DASPTF010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131554"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VEH48_02755"
     CDS             complement(<1..721)
                     /locus_tag="VEH48_02755"
                     /inference="COORDINATES: protein motif:HMM:NF019880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HXY18313.1"
                     /translation="MDAVAEIKGRLNIEDVVGQYVQLKRAGRNFKGLSPWTNEKTPSF
                     IVSPEKQIWHDFSSGKGGDIFGFVMEMEGLDFRGALEQLARQAGVDLEQFQGRSSGNS
                     KLKSRVHEALELATRFYQKHLIANPAALNYLKSRRFSRQTLLDWQLGYAPESGQAVTN
                     FLTKRGFSKDEIKRAGLAVDRRGQSVDMFRGRIMIPLSDSRGAVVGFTARLLKDEPDA
                     PKYINTPQTIVYDKSRNVFGLH"
     gene            788..1423
                     /locus_tag="VEH48_02760"
     CDS             788..1423
                     /locus_tag="VEH48_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18314.1"
                     /translation="MPPQTPSQPVSSPSPNPSYDFILRDPQAPRRRFSLPGGSGAMKI
                     LFGLLGVLVIVIIFAVVAGGGGPSNAQQLVAVAGRAQEIARVSSLLQQQSNDPNTQYL
                     AATAQIALTSEQYRITGYLKAHKVKVSPAQLAAYQNKNTDSQLITAKQNNDLEETFDA
                     YLKSSLEAYQASLQTASKGASKTVLPILQDANASTQVLLAAPEITSLSTNS"
     gene            complement(1581..1898)
                     /locus_tag="VEH48_02765"
     CDS             complement(1581..1898)
                     /locus_tag="VEH48_02765"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HXY18315.1"
                     /translation="MNHKQVEKYLLSMPEAKLDYPFGEEVAVYKANDKMFALVREKKM
                     PVQLSLKCDPLLAQTLREKYETVMPGYHLNKKHWNTILLTGQLSWDEVKDLINHSYQL
                     VVS"
     gene            complement(1898..2347)
                     /locus_tag="VEH48_02770"
     CDS             complement(1898..2347)
                     /locus_tag="VEH48_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18316.1"
                     /translation="MERVETPAASAAIELDTIQNRRNKARELYRWTEEVADYAREVAS
                     EGSWSDRAFGPTLEGGWESLDYDFVGTNFVPITDGVSVAIREIAREVGGQPRHELLMQ
                     SDEGAEIEVLARIDGPHIEIDGVGKPEFHEVSAIKGLLDYIAGHETR"
     gene            complement(2394..2804)
                     /gene="msrB"
                     /locus_tag="VEH48_02775"
     CDS             complement(2394..2804)
                     /gene="msrB"
                     /locus_tag="VEH48_02775"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXY18317.1"
                     /translation="MAKQFTDEELKKKLTPEQYHILREKGTEAPFTGKLLHNKEDGTY
                     VCPVCGNELFSSSTKFDSGTGWPSFYDVASTGSVKLVEDNSGGMRRVEVLCANCGSHL
                     GHVFNDAKGQPTGMRFCINSASLKFMSAKDSKKS"
     gene            complement(2808..3140)
                     /locus_tag="VEH48_02780"
     CDS             complement(2808..3140)
                     /locus_tag="VEH48_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HXY18318.1"
                     /translation="MSKQIRGFMDFVRTQGVVGLAVGLAIGTQVTATTKSIADGFINP
                     IVGFILGSTTALSADSWHLSLGHRNMVIGWGAILSSLITLVAVAAVIYFVVKGLGLDK
                     LDKQKEEA"
     gene            complement(3164..>3451)
                     /locus_tag="VEH48_02785"
     CDS             complement(3164..>3451)
                     /locus_tag="VEH48_02785"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HXY18319.1"
                     /translation="STLHSPLSTMEQDERSLARKISEYPEVVAKATAELMPHHICTYL
                     YELAQAFNHFYEKNRVIDDPRQDLRLRLVEAYANVLKRGLGLLNIAAPEKV"
BASE COUNT          736 a          954 c          875 g          886 t
ORIGIN      
        1 ggtggaggcc gaagacgttg cggcttttgt cgtaaacgat agtctggggc gtgttgatgt
       61 atttgggtgc atccggctcg tctttcagca ggcgggcggt aaagcctacg accgcgccgc
      121 ggctatcgct taggggaatc ataatccggc cgcggaacat atcgactgat tggccgcggc
      181 ggtctacggc caggccggcg cgctttatct catccttgct gaagccccgt ttggtcaaaa
      241 aattagtgac ggcctggccg gactccggcg cgtagcccag ctgccaatcc agtaaagtct
      301 gccggctgaa tctgcggctt ttgaggtagt tgagcgccgc tggattggca atcaggtgtt
      361 tttggtaaaa ccttgtcgcc agttccaatg cttcgtgcac ccggcttttg agcttactgt
      421 tgccgctgct ccggccttgg aactgctcca agtccacgcc ggcctggcgg gccagctgtt
      481 ccagcgcgcc gcggaaatcc aggccttcca tctccattac gaaaccgaaa atatcgccgc
      541 ccttgccgga tgagaagtcg tgccatattt gtttttccgg gctgacaatg aagctcggcg
      601 tcttttcgtt ggtccagggg ctgaggcctt taaaattgcg gccggcgcgc ttaagctgga
      661 cgtactgccc gaccacgtct tcaatattca accggccctt aatctccgca accgcatcca
      721 tatctttaag tataaggctt gtcagttaga ggttacagca taagcgttat cttgctaaaa
      781 tggtcttatg cctccgcaga ctcctagcca gcctgtatca agcccttcgc caaacccgag
      841 ttacgatttt atattgcgcg atccacaggc gcccaggcgg cgctttagcc tgcccggcgg
      901 ttccggcgcc atgaaaatct tgttcggcct gctgggcgtt ttagtgatcg ttattatttt
      961 tgctgtggta gccggtgggg gcggcccaag caatgcccag cagctagtgg ccgtggccgg
     1021 ccgggcgcag gaaatcgccc gggtgagcag cctgttgcag cagcagtcaa atgaccccaa
     1081 tacccagtac ctggcggcca cggcccagat cgccctgacc agcgagcagt accggatcac
     1141 cggctattta aaggcgcaca aggtcaaagt ttcgcccgcc cagctggcgg cctaccaaaa
     1201 caaaaatacc gacagccagc tgataaccgc caagcagaac aacgacctgg aagaaacttt
     1261 tgacgcttat cttaaaagta gcctagaggc ctatcaggcc agcctgcaga cggcctccaa
     1321 gggcgccagc aaaaccgtcc tgcccatttt gcaagatgca aacgccagca ctcaggtact
     1381 gctggcggca ccggagatta cctcactcag taccaatagc taaatcgtag ctcagccgga
     1441 tcaagtcttt gatctcttcc caactcagct ggccggttaa tgcggcttcg tttcactccg
     1501 ccttatgagg acaaaccaac ggtttttcct catcgcgccg agcaaaaagt gtgatttttg
     1561 ctcgggctgc tctttttcct ttagctaacg accaattggt aggaatgatt tatcaaatct
     1621 ttcacttcat cccaagaaag ttgacctgtt aaaaggatgg tattccaatg ctttttgttg
     1681 aggtggtagc cgggcatgac ggtctcgtat ttttcgcgca aagtttgagc caaaagcgga
     1741 tcgcatttta gactgagctg cactggcatt tttttctcac gcaccagggc gaacatctta
     1801 tcgttagctt tataaaccgc gacctcttcg ccgaacggat aatccagctt ggcttccggc
     1861 atactcagca gatatttttc gacctgtttg tggttcacta tctagtctcg tggccagcaa
     1921 tatagtctaa taatccttta attgcactga cttcatgaaa ttctggcttg cccactccat
     1981 ctatttctat atgtggacca tcaattctag ctagtacttc tatctcggca ccctcgtcag
     2041 actgcatcaa gagttcatgt ctgggttggc ccccaacttc acgagctatt tccctaatag
     2101 caactgagac cccatccgta atgggaacaa aatttgtgcc cacgaagtcg taatcaagtg
     2161 attcccatcc tccctctaag gttggaccaa atgcccggtc ggaccaggaa ccttcgcttg
     2221 caacctctct tgcatagtcg gcgacttcct cagtccacct atatagttct ctagctttat
     2281 tccggcgatt ttggatagta tctagttcta tagctgccga tgctgccggg gtttctactc
     2341 tttccatggc tatatcatat agtgtgctgg ttaagatact caagcagtag cgtttacgat
     2401 tttttggaat ctttggcgga catgaacttc aggctggccg agttaatgca gaacctcatg
     2461 ccggtcggct ggcccttggc gtcgttgaat acgtgcccta aatggctgcc acagttggca
     2521 caaagaacct ctacccggcg catcccgccg ctgttatcct ctactaattt gacggaaccg
     2581 gtactggcaa catcgtagaa actgggccag ccggtgccgg aatcgaactt ggtgctgctg
     2641 gagaatagct cgttgccgca gaccgggcag acgtaagtgc catcttcttt gttatgcaag
     2701 agcttgccgg taaatggcgc ttcggtgccc ttttctcgca agatgtggta ctgctccggc
     2761 gtcagctttt tcttcagctc ttcgtccgta aactgcttgg ccacctttta ggcttcctct
     2821 ttctgcttat ccagtttgtc taagcccagt cccttgacta caaaatagat gacggccgcc
     2881 acggctacca gagtgattag agacgacaat atcgcccccc agccgatgac catgttcctg
     2941 tgccccagcg aaaggtgcca gctatccgct gatagggcgg tggtgctgcc gaggataaaa
     3001 ccgactattg ggttgataaa accgtcggcg atgcttttag tggtggccgt aacctgcgta
     3061 ccgatggcca gaccgacggc caggccgacc acaccttggg tgcggacaaa atccataaaa
     3121 cctctgattt gtttgctcac gttgacctcc tatttggctg ttattatacc ttttctggcg
     3181 ccgcgatatt cagcaatccc agcccgcgct tgaggacgtt ggcataagct tctaccagcc
     3241 ggagccttag gtcttggcgc gggtcgtcaa taacccggtt tttttcgtaa aaatggttga
     3301 acgcctgggc cagctcgtat aaataggtgc agatatggtg cggcatcagc tcggccgtgg
     3361 ctttggcgac cacctccggg tattcgctga ttttacgtgc taagctgcgc tcatcctgct
     3421 ccatggtgga gagtggagag tggagagtgg a
//
LOCUS       DASPTF010000083         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_132694, whole genome shotgun sequence.
ACCESSION   DASPTF010000083 DASPTF010000000
VERSION     DASPTF010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132694"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VEH48_02790"
     CDS             <1..614
                     /locus_tag="VEH48_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXY18320.1"
                     /translation="YSGTAIFTKTEPKQVINDLPAELAKKYNFADERNRNAANEGRVI
                     AAEFDAFWLVDVYTVNAVDDLSRVELRHKHWDPAFLAYVKGLEKKKPVIFCGDLNVAH
                     TKDDLARPKENVGKRGFTNEEREGFQNYIDAGFVDTFRLFHKGNGFYTWWTHWANARE
                     RNVGWRIDYFLVSAKLKSKVKSAAIHPEIMGSDHCPVSIELDL"
     gene            611..1174
                     /locus_tag="VEH48_02795"
     CDS             611..1174
                     /locus_tag="VEH48_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18321.1"
                     /translation="MKTVAIIYGWTGGSWSTRHFRDELQKAGFKVISNREKANIIIAH
                     SAGCYRVPDKTSAQLILLIGVAYWPEKRLRSRAMNRFINGLKEDRDRGVLYIFHRFMW
                     QLFYAIIRPGDIVISLRNHKSLDFLEKLKDKRIILIRNSRDEYCSPNIARSITKYSNI
                     KFVELLGLHDDYYTNPKPYIDLLLKAL"
     gene            1182..1913
                     /locus_tag="VEH48_02800"
     CDS             1182..1913
                     /locus_tag="VEH48_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXY18322.1"
                     /translation="MARGRKFAVFDIDGTLIRWQLYHALADTLARQGHIKPDTYQAMK
                     DARMEWKRRTGGGFKSYELQVIEVYEAVLKTLTFKQLEEGIKSVFEEYKDQTYTYTRD
                     LIARLKKQNYKLFAISGSQIEIVAKIADYYGFDDYVGTVYQQKGDGFSGDKTIGSLNK
                     DQTLKTLAKKHGCGFKGSVAVGDSHSDIVMLKLVQLPIAFNPEDSLFKSARRYGWKIV
                     LERKNMVYELEKKDGRYELVKTNAG"
     gene            1876..>2279
                     /locus_tag="VEH48_02805"
     CDS             1876..>2279
                     /locus_tag="VEH48_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18323.1"
                     /translation="MEDTNWLRQTPDKPLFPDLLWSRPENKRQRGKLLIVGGNSHGFA
                     APASAFAAAGTAGIGAARVLLPDATQKTIGTGFTDAVFAPSTPSGSFSRQALAELLEN
                     AGWADGVLLAGDFGRNSETAILLESFLDKYKG"
BASE COUNT          636 a          542 c          585 g          516 t
ORIGIN      
        1 gctattccgg cacggccatc tttaccaaga ccgaacccaa gcaagtcatc aacgacttac
       61 cggcagagtt agccaaaaaa tacaatttcg ctgatgagcg caaccgcaac gccgccaacg
      121 agggcagggt aatcgccgcc gaatttgatg cattctggct ggtcgatgtt tacaccgtaa
      181 acgccgttga cgacctaagc cgggtagagc tccggcataa acactgggat ccggcatttt
      241 tggcctacgt gaaggggtta gagaagaaaa aaccggtgat tttttgcggc gacctgaacg
      301 tagcccacac caaagatgac ctggcgcgac ccaaagagaa cgtcggcaag cgcggtttta
      361 ccaacgaaga gcgcgagggt tttcagaact acatcgacgc cggttttgtg gacactttcc
      421 gcctgttcca caaaggcaat ggcttttaca cctggtggac gcattgggct aacgcccgcg
      481 agcgtaacgt cggctggcgg attgattatt ttctagtctc cgccaagctc aaatcaaagg
      541 taaaatccgc cgccatccac ccggaaatca tgggctccga ccactgccct gtgagcattg
      601 aacttgattt atgaaaacgg ttgccattat atatggttgg actggcggca gttggagcac
      661 gcggcatttt agagacgaat tacaaaaggc aggcttcaaa gttatttcaa atcgagaaaa
      721 ggccaatata attattgccc actctgcagg ctgttaccga gttcctgata agactagcgc
      781 gcaattaatt ttgttaattg gagtggcata ttggccggaa aaacgattaa gatccagagc
      841 catgaataga tttattaatg gtctaaaaga ggatagggat cgaggagtac tctacatatt
      901 tcatagattc atgtggcaat tgttttatgc aatcatacgc cctggggaca tcgttatttc
      961 actacgtaat cataaaagcc ttgatttttt agaaaagttg aaagataaac ggataatact
     1021 cataagaaac agtcgagacg aatactgcag tccgaatatc gccaggagta ttactaagta
     1081 ctcaaacata aaattcgtgg aacttcttgg acttcatgac gattactaca ccaaccctaa
     1141 accatatata gatttactcc tcaaagctct ataattagcg tatggcaagg gggagaaaat
     1201 tcgccgtttt tgacatagac ggcacattga tcagatggca gttgtatcat gcgctggccg
     1261 acacgctggc aaggcagggg catatcaagc ctgacactta tcaggcgatg aaagacgcgc
     1321 gcatggaatg gaaacgccgg accggcggcg gctttaaaag ctatgaactc caggttattg
     1381 aggtttacga agcggtgctc aagaccctga cgttcaaaca gctagaggag ggcataaaat
     1441 ccgtgtttga ggagtacaaa gaccagacct acacttatac ccgcgattta atcgcaagac
     1501 tgaaaaagca gaattataaa ctgttcgcta tttctggctc ccagattgag attgtcgcca
     1561 aaatcgccga ttattacggc tttgacgatt acgtcggtac ggtctaccag caaaaaggag
     1621 atgggtttag cggtgataag accatcggtt cgctcaataa agatcaaact ctgaagactt
     1681 tggcaaagaa acatggctgc ggcttcaagg ggagcgtggc cgttggcgac agccacagcg
     1741 acatcgtgat gctcaaattg gtgcagctgc cgattgcttt caatcccgaa gattcgctct
     1801 ttaagagtgc ccggcgatat ggatggaaaa ttgttctgga acgcaaaaat atggtctatg
     1861 agctagagaa aaaagatgga agatacgaac tggttaagac aaacgccgga taaaccgtta
     1921 tttccggact tgctgtggag ccggccggag aacaagcgcc agcgcggcaa gctgctgatt
     1981 gtcggcggca attcgcacgg ttttgccgcg ccggccagcg cctttgctgc ggcgggcaca
     2041 gccggcatcg gcgctgctcg ggtgcttctg ccggatgcca cccaaaaaac aatcggtacc
     2101 ggctttaccg atgccgtttt cgcacctagc acacccagcg gcagtttcag ccgccaggcg
     2161 ctggccgaac tgctggaaaa cgccgggtgg gccgatggcg tactgctggc cggcgacttt
     2221 ggccgcaatt ccgaaaccgc catcctgctg gaatctttct tagacaaata caaggggca
//
LOCUS       DASPTF010000084         4333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_272613, whole genome shotgun sequence.
ACCESSION   DASPTF010000084 DASPTF010000000
VERSION     DASPTF010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272613"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VEH48_02810"
     CDS             complement(<1..660)
                     /locus_tag="VEH48_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18324.1"
                     /translation="MTVLAVLVGFLILVGAGCGFAYFYLRTVFREQKNYERGLKMVPV
                     LIHLPPPSDDTESKGRDTRDVVDENISRAQTLYAILSSTFQKGLRHKFFGQRHVALEI
                     VANHGAVHFYAAVPVPLLSVVEQAIVSAYPTARLEEVPEHNIFSGVGRITGTVGGELQ
                     LKESFAYPIATYQEIKRDTMQSLLNALSGLTIEDGAGIQILLRPADGGWRKIATTEAS
                     KK"
     gene            complement(657..1085)
                     /locus_tag="VEH48_02815"
     CDS             complement(657..1085)
                     /locus_tag="VEH48_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18325.1"
                     /translation="MDPSSGEKSGPQFEAPQTAEGLEQRHDKAMERAPDGESAIGKKT
                     PKQLNTAVLQLPTDIPTAAPVAIPSDQPAGRPPAAEPPQTAARDSHQIEKQWVDKAKK
                     IIAQTKDDPKTQKHQMSQVKAEYIQKRFNKTIPTEGTAGP"
     gene            complement(1127..1474)
                     /locus_tag="VEH48_02820"
     CDS             complement(1127..1474)
                     /locus_tag="VEH48_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18326.1"
                     /translation="MNSNRHRARVPAALAAGCFLYLAAIMSFTSPVKNISWAIAFFIG
                     LAAFLVSLGYTAVYLQAGAMQPRYRLRIGIITVFILVLLMFRSAQSLSWIDGLVLLII
                     TLGLLFYSSHRAS"
     gene            1935..2462
                     /locus_tag="VEH48_02825"
     CDS             1935..2462
                     /locus_tag="VEH48_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXY18327.1"
                     /translation="MAAENDELLDLVNEHDVVIGTVPRGQKPSSDQPVFLRAAELFIQ
                     NSRGQLWVPRRQKHKQIAPGGLDYSASGHIGAGESYTEGLNREVKEELNLQIDPKQLK
                     FVHKFPPTSAEKMFFRSVYLYYSDDVPRFNPEDFSEYYWLTPQELLSKLENGEPAKRS
                     LQETVRYLIDHPVGS"
     gene            3137..>4333
                     /locus_tag="VEH48_02830"
     CDS             3137..>4333
                     /locus_tag="VEH48_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HXY18328.1"
                     /translation="MKESVRSIVDYQGTGGMAVEVECHITNGLPAVIIIGYASKAVDE
                     AKERLRASFANSTIQFPKKRVTINLSPADIPKDSTSLDLAMAVAVLARSQQIKTEALA
                     GYIFLGELSLDGSLKPVRGLIGRILAAKSLGFSHFYIPRQNLEQALLIPDITIKAAPS
                     LRDVYLDLSASLPLKAISSGRGRLKAEPTDVEALEFAEISGQPIAKRALEIAAAGHHN
                     ILLHGPPGTGKTMLARAMCGILPPLDHSEVLEVTHLHSLASGGFSQIITARPFRAPHH
                     TASATALVGGGSKPKPGEATLAHRGVLFLDEISEFSHDCLEALRQPLEDGSVSIARVR
                     DSATYPADFMLVATQNPCPCGYYGTDKPCQCSAAAIAKYQKKVSGPILDRIDLHLPVQ
                     GVDHKRL"
BASE COUNT         1040 a         1189 c         1127 g          977 t
ORIGIN      
        1 tttcttgctg gcctcggtgg tggctatctt gcgccagccg ccatcggccg ggcgcagcag
       61 gatctgaata cctgcgccgt cttcgatggt caggccggac agggcattaa gcagcgactg
      121 catggtgtcg cgcttgatct cttgataagt ggcgattggg taggcaaagc tctctttgag
      181 ctgcagctcg ccgccgacgg taccggtgat gcggccgacg ccgctgaaga tgttatgttc
      241 cggtacttct tccagccggg cggtcggata agcgctgaca attgcctgct ccaccacgct
      301 cagcagcggt accggtacag cggcataaaa gtgtaccgcg ccgtgattgg cgacgatttc
      361 caaagctacg tggcgctggc cgaagaactt atggcgcagc cccttttgga aggtgctgga
      421 caggatggca taaagcgtct gcgcccgcga gatgttctcg tccaccacgt cgcgagtatc
      481 gcgccccttg gactcggtgt catcgctggg cggcggcagg tggatcaaca ccggcaccat
      541 cttcaaaccg cgctcgtagt tcttctgttc gcggaaaacc gtccgcaggt aaaaataggc
      601 aaaaccgcag ccggcgccta ccaaaatcaa gaagcccacc aaaacggcta agacggtcat
      661 gggccggctg taccttccgt ggggatggtc ttattgaagc gtttttggat gtactcggcc
      721 ttgacctggc tcatctggtg tttctgggtt ttggggtcgt ctttggtctg ggcaatgatt
      781 tttttagcct tatccaccca ttgtttttct atctggtgcg agtcgcgggc ggcggtttgc
      841 ggcggctcgg cagccggcgg gcggccggcg ggctggtcag acgggatagc taccggcgcg
      901 gccgtcggaa tgtctgttgg cagctgcaaa acagcggtgt taagctgctt gggcgtcttc
      961 ttcccaatag ccgactcgcc atccggagcc cgctccatgg ccttatcgtg ccgctgctct
     1021 aagccttcgg cagtctgcgg cgcctcaaac tgaggcccgc tcttttcccc gctacttgga
     1081 tccatagctt tattatacta aagtttgatt gtccgtggtg cgacttttag gacgccctgt
     1141 gactactgta gaagagtaat cctaaggtga tgataagcaa taccagccca tcaatccagc
     1201 tcaggctttg ggcgcttctg aacatcagca gcaccaggat aaaaacggtg attataccga
     1261 tacgcaagcg gtagcgcggc tgcatagcgc cggcctgcag ataaaccgca gtgtagccca
     1321 aactgaccaa aaacgccgcc aaaccgataa aaaaggcgat ggcccagcta atgtttttga
     1381 ccgggctagt aaagctcatt atggccgcca gatataaaaa acaaccagca gccaaagctg
     1441 ctggtactcg ggcacggtgg cggtttgagt tcacgccatc cattatagaa cgaggaggct
     1501 tagcgggtaa atgcgctcaa tataaattac ttaatagcgc ttaccatcgc taagccattg
     1561 actgaccgct ctctatgcga gagcgacggc taaaacggtc tccactaacc gctagatata
     1621 agttattttt tgtttttaac ttatgctgac catactagca aaaaatagtt aaaacgtcaa
     1681 catcttatac atatattttt tacaataata cagcttaaaa ttacaactta tccacagatt
     1741 agttagcttc taatatgttg tattttaatg aaagttgtaa ataatatgtt ctttctttgt
     1801 tcgtttttta gtgttatggg ccagcacaaa ttcggctcat tcaccgtctt gcttcaagtc
     1861 caaatattaa tcatggttcc gacacaaaaa cgacgtaatt tcgccccttt ttttgttaaa
     1921 attaaagcaa agctatggcc gctgaaaacg acgaactttt ggacttagtc aatgagcacg
     1981 atgtggtcat cgggaccgtg ccgcgcggcc aaaagcccag cagcgaccag ccggtttttt
     2041 tacgagccgc cgaacttttt atacaaaata gccgcggcca actttgggtg ccgcgccggc
     2101 aaaaacacaa acaaattgcg cccgggggcc ttgactacag cgccagcgga cacattgggg
     2161 ccggcgaatc atacaccgaa ggcctaaata gagaagttaa agaggaatta aacctgcaaa
     2221 ttgaccccaa acagcttaaa ttcgtacata aattccctcc cacgagcgcc gaaaagatgt
     2281 tttttagatc agtttatctt tattactccg atgatgtgcc ccggtttaac cccgaagatt
     2341 ttagcgagta ctactggtta actcctcagg agcttcttag taagctggaa aacggcgaac
     2401 cggctaaacg cagtctgcaa gaaacagtgc gttatttaat tgaccacccg gtaggctcgt
     2461 agggtctacg ccggcaagcc cagccagcca aaacgctgat tttgtccgat tagccgccgc
     2521 cagaccgcgg cgcccgtgtt tgatccaagc caaagcgcgg cggggctggc caaaagccgg
     2581 tctaagccag ctatttttgc cgcgggtcgg cagcattgcg ggctgaagcg ccagccatcg
     2641 agctagaagt tatgcacagg tttgttgaaa aaaccataaa aatcttgttg acaggctttt
     2701 cagtagttta taagatgagg atggcacttg aataaaaaaa gtgctcaaaa caaaaactga
     2761 tctgtaacaa ccgcttctcg caagcggttg ttccatccaa aaccaactta taacaaaaac
     2821 cagcaaacac atctccaaac ccaaccaaaa ctcacaaaac gcctgcccaa aacgcaggcg
     2881 ttttgtttta taaacgccaa aaatcgtttt atgaaatgcc tcaacaagcg cttttaataa
     2941 ggcgagggac tttctgttgg ggccgcgagc acctcgtatt gaaaatacat gggtttgcct
     3001 agtgcaaccc gagtgcgtta cgcacccggc gtgtaacggg tattttcagt acgaggtgcg
     3061 ggaggcccct gctaaagttt ttaaacacta gcaattaatt ttttggggtt gaggtgcgat
     3121 aattcagctt aatctcatga aagagtcagt caggagtatc gtcgactacc agggcaccgg
     3181 cggcatggcc gtggaggtag aatgccatat caccaacggt ctgccggcgg tcattatcat
     3241 cggttatgcc tccaaagccg tggatgaggc caaggagcgg ctgcgcgcca gttttgccaa
     3301 cagcaccatc cagttcccta aaaaacgggt gactatcaac ctgtcgccgg ctgacatccc
     3361 caaagattcc accagcctgg acctggccat ggccgtggcc gtgctggcgc gcagccaaca
     3421 gatcaaaacc gaggctttgg ccggctatat tttcctgggc gagctcagcc tggacggcag
     3481 cttaaagccg gtacgcggcc tgatcggccg gatattggcg gccaagagcc tgggcttcag
     3541 ccacttttat atcccgcgcc agaatctgga acaggcgctg ttgataccgg atataaccat
     3601 caaggcagcg cccagcctgc gcgacgtcta cctggacctg agcgccagcc tgccactgaa
     3661 agctattagc agcggccgcg gccggctcaa agccgagcca acagatgtag aagccctgga
     3721 atttgctgag atttccggcc agccaatcgc caaacgggcg ctggaaatcg ccgccgccgg
     3781 gcatcacaac atcttactcc acggcccgcc gggcaccggc aagaccatgc tggcgcgggc
     3841 catgtgcggc atcctgccac cgctggacca ttcagaggtg ttagaggtta cccatttgca
     3901 cagcctggcc agtggcggat tcagccagat catcactgcc cgccccttcc gcgcgccgca
     3961 tcataccgcc agcgctactg ccctggtggg tggtggttcc aaacctaaac ccggtgaggc
     4021 cacactggcc caccgtggcg tactttttct tgacgagata tcggaattca gccacgattg
     4081 cctggaagcc ctgcgccaac cgctggaaga tggcagcgtc agcattgccc gggtgcgtga
     4141 cagcgctacc tacccggccg attttatgct ggtggccacc cagaatccct gcccttgcgg
     4201 ctactacggc accgataaac cttgccagtg ctcggccgcg gccatcgcca agtatcaaaa
     4261 gaaggtatcg gggccgattc tggaccgcat agacctgcac ctgccggtcc agggtgtgga
     4321 ccataagcgc ctg
//
LOCUS       DASPTF010000085        12985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_273109, whole genome shotgun sequence.
ACCESSION   DASPTF010000085 DASPTF010000000
VERSION     DASPTF010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 12985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12985)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12985
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273109"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..447)
                     /locus_tag="VEH48_02835"
     CDS             complement(64..447)
                     /locus_tag="VEH48_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18329.1"
                     /translation="MRRRIIIIIALVVAAFVVAVALFFFGDEHAVSSMSIKQVTPDQL
                     AAAMKEDHFYSDYRQNTLLIRGQVASVIGSLLTFKTSSSYQAQCSLAISSLNIHKGDN
                     VTVISEGGTAVRQPSGVLLKDCVIP"
     gene            1016..1639
                     /locus_tag="VEH48_02840"
     CDS             1016..1639
                     /locus_tag="VEH48_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HXY18330.1"
                     /translation="MFVFIDDAGDAGFKLDKGSSSYFVIACVIFDDNLEAEETALRIK
                     KLRRSLGWRDEHEFKFNKTSKKVRLAFLEEIKGCNFRVRAIVADKSIIRSHELRNNKE
                     SFYNYMIKEVLSKSDGFIKDARIRLDGHEDRAYKKAATTYFRQFANPRGKVIKNMRFV
                     NSKSDSLIQLADMVAGAILRTTQKDKTDNQNYLKVINKRLEDLWHFK"
     gene            1847..2563
                     /locus_tag="VEH48_02845"
     CDS             1847..2563
                     /locus_tag="VEH48_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18331.1"
                     /translation="MPNETPPDGTTEGLGNATTNESSTQIPVTDGSENLYPDRIEDPE
                     KARAMAEAADPRMENALYWKNEKTEAQENADQLKEVLDTQSYDGDEKVFGKALKKVHP
                     DRGYAPTWGNVQSVESKQREIAESSAAREAQARAWADEDAEKAGDIWDIAYGESGAEE
                     KRIHNIDKARVMAEAADFWMSVAVRENKPELKEWAAQHAYDAGKLFDQAGNLRAYRKL
                     VEKKEAVEEERRRLSAGRKY"
     gene            complement(2560..3960)
                     /locus_tag="VEH48_02850"
     CDS             complement(2560..3960)
                     /locus_tag="VEH48_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18332.1"
                     /translation="MLGLNHTLKIKMPTQELVPDMSEITASSEQELNPDISQYELAFK
                     ARQELVNQTLQALKMTHYGQKFATVESIGSYSEFVTTFPLVLPSRAAEDPKQFAAGDT
                     DKLIKLYSGGTTGTQKEFYFSDEERVNASGLGEWGRIILAAEKPLVLYGEWGGIDAQV
                     AKETTQALRPDVGVYEFKETHDAIEAIAEHDAIFMEMGVMSYKTFHYQLMEQLAARPE
                     LKQQLSGKKIMLELNSEPVTAKMLEEWYADLATIFEDEVYITVSYTQNDTGLIGLYDY
                     VPGEAEEDVKYLVTDKRLVEVLDEDSNLVVGQEGDVVITTLRQEGSVFFRYDSSDRGV
                     LTSGENGELYLSGIHRKAESGMISLWGRKVSVADFYQELETQVSFPFQMEVRPQLSED
                     NSVMELVFAIYCQSDDEGIADLKEHFWQLAEKMIAAEDDTTLAQGDVPLDVDVVIRIK
                     RDTKSNFEKSWKLIKN"
     gene            complement(4034..4110)
                     /locus_tag="VEH48_02855"
     tRNA            complement(4034..4110)
                     /locus_tag="VEH48_02855"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4074..4076),aa:Gly,seq:gcc)
     gene            complement(4296..5834)
                     /locus_tag="VEH48_02860"
     CDS             complement(4296..5834)
                     /locus_tag="VEH48_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18333.1"
                     /translation="MKYKYDFKKGITKARTKLAIAVSSLALIGGGVGLSLTLFGTAHA
                     ISPSLWEINAPEAITFVCGGGDYNHTLNTVSEDSSGSFSGTGTYNPDNTYTWNINGNV
                     SGNNLTFTIVYTGTNAGYTLDGIGTIAPDGSITGTVDNNCQTFSMPAGTATPFCYPVT
                     TSKGLLTAAQIGGDVTGDLNASGCDIGVYYNGSDTGNVSGASIHDANKYGVFVDGISG
                     NLSVKVTNSHVYNVGNHTSGVYSPNGVQTGIDIYYYGLNTSGKVSADINHDLVDNYQK
                     GGIVINGQNAKGKVDKNTVNGAGPVDYIAQNGIQIGFGATGEITSNKVTGNAYTGTNF
                     ASSGGILLFGGLGDPLVTNVKVKDNKLVNNDVGIFMVNYNAGGTGPATTPTKDMAVDN
                     WISDSTVTNVSGLCNNDPSCGGGFIGYQAGINDVGNKDFACNNNISGTGYADQGNYNY
                     IPTPPVFTQTGPNRAVVRHIDAGDTFPTTDFSTCHNDHFFNEHYNNQFFKFYHNRFGH
                     WYFH"
     gene            5957..6032
                     /locus_tag="VEH48_02865"
     tRNA            5957..6032
                     /locus_tag="VEH48_02865"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5990..5992,aa:Phe,seq:gaa)
     gene            complement(6171..6932)
                     /locus_tag="VEH48_02870"
     CDS             complement(6171..6932)
                     /locus_tag="VEH48_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HXY18334.1"
                     /translation="MIVLLVVLVLVLLIALAGIRIVNQYQRGVVLRLGKLRPDIKEPG
                     LNFIIPIVDQMRRVNLRIITLPIESQKIITKDNVSIDVAAVAYYKVSDPIKSVVEIEN
                     VLDATYQIAQTTVRNVVGQSSLDEVLSATAAINEKIKKILDTATAKWGIYVSTVELKD
                     IQLPDSMQRAMARQAEAEREKRAKIIAAEGEKLSATGLGEAADIISKHPIALQLRNLQ
                     TLVEIADEKNSTVVFPAQFMSTVEDIKNFLAREKK"
     gene            complement(6951..7027)
                     /locus_tag="VEH48_02875"
     tRNA            complement(6951..7027)
                     /locus_tag="VEH48_02875"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6991..6993),aa:Val,seq:tac)
     gene            complement(7029..7115)
                     /locus_tag="VEH48_02880"
     tRNA            complement(7029..7115)
                     /locus_tag="VEH48_02880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7079..7081),aa:Leu,seq:gag)
     gene            complement(7168..8967)
                     /locus_tag="VEH48_02885"
     CDS             complement(7168..8967)
                     /locus_tag="VEH48_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HXY18335.1"
                     /translation="MKSSIPKLDDDTKRRWRRRFNRRRRGAIQLGQQADASIEKLIIR
                     RFDRLVSVRRFVLLWVGLFVLLILISVAQLRNLSAYYQVLKPVPGGLYNEGLVGSFTN
                     ANPIYATGAADTAVSRLVFSGLFKYNDDNQLVGDLANGWTLSANQTDYVVHLKKNIFW
                     QDGRPFTADDVVFTYHTIQNANAQSSLISSWKDITVTKQSTYSVNFSLPNPLSSFPYA
                     LTNGILPAHLLKSVPPEQLRSAAFNTAPVGTGPFELKFINVTGNTTADRQQRIALAAY
                     DRYYAGAPKLDGFSIITFSDDNHLIAAFKQKQLNAITGLESVPAELAGDSSVQTYTTP
                     LTSAVMAFFNNSRPLLSDANVRKALEESVDRAQLESLFDTPVKPVDGPLLRNQLGYDP
                     SIVEASYNFAAASQTLDQAGWTVGQGGIRYKNGQPLSFVLKSQDTPQYTRVAEFLQRQ
                     WAKLGVKVTAQYYDADDLQSIVIANHDYDALLYGINIGVDPDVFAYWDSSQASVSSQG
                     HLNLSEYKSAAADQALEAGRTRSDPAVRVLKYKAFLTAWVQDVPALALYQPNLLYITH
                     GPVFNYQRKADNGELDQFYNVNQWEVRQQKQDI"
     gene            complement(8980..9195)
                     /gene="secG"
                     /locus_tag="VEH48_02890"
     CDS             complement(8980..9195)
                     /gene="secG"
                     /locus_tag="VEH48_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXY18336.1"
                     /translation="MTIWNYITIVSVIILTILILVQTRGASLGAGLGGAGEVNTVRRG
                     SDKTLHQLTIIMVFIFAASIIINIVAK"
     gene            complement(9192..9572)
                     /locus_tag="VEH48_02895"
     CDS             complement(9192..9572)
                     /locus_tag="VEH48_02895"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXY18337.1"
                     /translation="MKFLLRWLVSSLGLWIAEAILGSSRLSVGGSWKTVLAAGFFLAL
                     VNMFVKPFLVFLSIPAILVSLGLFMLVVNGFLILAASWLYHPLYVKDFGVAIIAGLIV
                     GLVNFLVTRVLDDTIKPISRRSRA"
     gene            complement(9630..11069)
                     /locus_tag="VEH48_02900"
     CDS             complement(9630..11069)
                     /locus_tag="VEH48_02900"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HXY18338.1"
                     /translation="MNKRAESKLKNLPTSPGVYFYKDNSGEIIYIGKAANLRNRVRQY
                     FQKSRARDPKTDALVTEITDLDWTELENEADALFLEAELVRRYQPRFNILLRDDKSLQ
                     YVRIDYGSDYPTVAMVRRPLDDGARYFGPYISGFAVKKALRYLRRAFPYAISRPAGQK
                     RASLHYHLGLDPGLEEGRTSLADYRANLRKLMQYLRGRRVALIREIERDMKKAAKAKN
                     FEEAARLRNQLFALRQLSRQILFSDHELQDASKDQALVGLAELLGLPAPPRRIEGFDV
                     SHLQGTDTVASMVVFLNGVPEKTAYRKFKMRLPGNDDFAHINEALSRRLSETNRQKWG
                     LPDLFLIDGGKGQLAAALKARDAADLTTPMIGLAKREEEIIMKLPAAKSFTILKLPEQ
                     SPLVKLLQRIRDESHRFAISYHSTIKRRRGTASLLDEIPGVGPATRKKLIRRFGSAKG
                     VMDASVAELQELLGVQTGQKIAAYLKTLR"
     gene            11442..>12985
                     /locus_tag="VEH48_02905"
     CDS             11442..>12985
                     /locus_tag="VEH48_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXY18339.1"
                     /translation="MSTTRKTLSIYWQHTLRYRWLFAAGTSGAAAAVIVQDIVPPFIV
                     AKAFNTLQSVYSSGGQLKFSELSGYLIAYLIFMLGGVILWRAQVICVWLYEMKVLRDI
                     ANRVFDFLQHQSYRFHANHFAGALVTQANRFINSYESLMDSFIWDITSGATTLVASII
                     FLLFVSKVYSLILLAISLVYLSIIYHRARGQIPYNRRESARQSDQTAALADVITNVGT
                     VSTFAAEKREYQRFAKVTEAKYSASHALMVYGMKTEIMSHFMTASINITALVTGVLAI
                     TTFHARVSVLVLAVSYTQGITGRLWQFGRVVRNINKALGDAAEMTEMLQQEPEIKEPA
                     SPKKLRIENGSINFDHVEFGYADGQGQVVLPNLSLSVPGGKKVGLVGYSGGGKTTISK
                     LVLRLMDIQKGSLTIDGTDIREVALADLRTKIAYVPQEPLLFHRTISENIGYAKPDAS
                     MAEIVQAAKMAYAHEFIKDLPDGYDTLVGERGIKLSGGQRQRIAIARAMLKNAPILVL
                     DEATSA"
BASE COUNT         3310 a         3152 c         3236 g         3287 t
ORIGIN      
        1 aagttaaagc gctttaactt aaaaatctcg ctcgccttcg ggcgagattt tttgttcaaa
       61 aactcagggg atgacgcagt ctttcagcag tactccactc ggttgcctga cggctgttcc
      121 gccctcagaa atgacggtga cattgtcgcc tttgtgaata tttaggctgc tgatagccag
      181 actgcactga gcttggtatg aagagcttgt tttaaaagtc agcaaagagc cgatgacaga
      241 tgcgacctgg ccgcggataa gcagtgtatt ctgccgatag tcgctgtaga aatgatcttc
      301 tttcatagcg gccgccagct ggtccggagt cacctgctta atgctcattg agctcacggc
      361 gtgttcatcg ccaaaaaaga aaagcgcgac ggcgacaaca aacgccgcaa ccactaaagc
      421 gataattata attattcttc tgcgcaccat tttaattata cccgcagccg taattttaac
      481 gcggcaagca ggcggatcgg gagttacatt aaggcgggtc cgctgtatga ttaagtaagt
      541 attaaattaa gcgtggagtg tcaatgagta tctggaattg gctattcgtt aatcagccga
      601 catgggtgtc aaatttatcc aatttaacat ctataacggt tttcggagcg gtcattggcc
      661 tgtataagcg tttcaactgc aaccaaccga ggtgctggcg tattggccac cataaggtcg
      721 agggaacgac ttatagaacc tgctcaaagc atacgacttt agaatttcat acagccctac
      781 aaaagaagca tgaaaaagaa cggcccgaac agcataaatt cttgaaccag cacaaaaaat
      841 ctgaggcttt atagtactga tacttggtgc acgatagttg acgcattaca aacatcagca
      901 attgctgccg ttaatgatta taaactaaaa catttggcag agacatttaa cctatcgcca
      961 gatttaatag gttagctgtt tgtgccaaat aagcagataa aggataattc aaaatatgtt
     1021 tgttttcatt gatgacgctg gtgatgctgg ctttaagcta gataaaggct caagtagcta
     1081 ttttgttata gcctgcgtta tctttgatga taaccttgaa gctgaagaga ccgcccttcg
     1141 cattaagaag ttaaggcgct cacttggctg gcgtgatgaa cacgaattta agtttaataa
     1201 gaccagcaaa aaggtcagat tagctttcct tgaagaaatt aagggctgca atttccgagt
     1261 tagagctatt gttgccgata agtctatcat ccgtagccat gaattaagga acaataaaga
     1321 aagcttctac aactatatga ttaaagaagt tttatcaaag agtgatggct ttataaagga
     1381 tgctcgcatt aggcttgatg ggcacgaaga tagagcctat aagaaagcgg ctactactta
     1441 ctttcggcag tttgctaatc caagaggcaa agtcattaag aatatgcggt ttgttaattc
     1501 caaaagcgac agtcttatac aactagctga tatggtcgca ggagctattt tgcgaactac
     1561 tcaaaaagat aaaactgaca atcagaacta cttaaaagtt ataaataaaa gattagaaga
     1621 cctctggcat ttcaaataaa aaaacccgcc tcctattctg gttgtcccag acacgcacac
     1681 catacggtga cagttcggaa agcgagtcaa ttgtgcttac agtataacga aaataagctc
     1741 actcgtcaat cataagctat ataaagaatg ttaagtagtg ttgacttctt tatcgctaat
     1801 gctaatattt aagcagacat taaaagacaa aaactagaaa gcaaaaatgc ccaacgaaac
     1861 accgccagat gggacaacag aaggattagg taacgcaact accaacgaaa gttcaacaca
     1921 aattccagtt acggatggaa gcgaaaatct ataccctgat agaattgaag accctgaaaa
     1981 agcacgagca atggctgaag ctgcagaccc tcgtatggaa aacgcacttt actggaaaaa
     2041 tgaaaagaca gaggcacagg aaaatgccga ccagctgaaa gaggtgctag atacacagag
     2101 ttatgatggt gatgaaaaag tttttggcaa agctctgaaa aaagtacacc cagatagagg
     2161 ttatgcaccg acttggggca atgttcaaag tgttgagagt aagcaacgtg aaatagccga
     2221 atcctctgct gctagagaag cacaggctag agcgtgggct gatgaagatg ctgaaaaggc
     2281 tggtgatatt tgggatatag cttatggcga atcgggtgcg gaagaaaaaa ggatacataa
     2341 catagacaaa gctagggtaa tggccgaagc cgcagatttt tggatgagtg ttgctgtaag
     2401 agaaaacaag ccggaactga aagaatgggc tgcacaacac gcatatgatg ctggaaagct
     2461 attcgaccaa gctggtaacc taagagcata caggaaactt gttgagaaaa aagaagcagt
     2521 ggaagaggaa agaagacgac taagtgctgg aagaaagtac tagttcttaa tcagtttcca
     2581 gcttttctca aaattgcttt ttgtatctct ttttattctt atcactacat caacatctaa
     2641 cggaacgtca ccctgagcta aggttgtatc gtcttctgcg gctatcattt tttctgctag
     2701 ctgccaaaaa tgctccttca aatccgctat cccctcatcg tcactttggc aatatatagc
     2761 gaaaaccagc tccatcacgc tattatcttc ggaaagttgt ggacgaactt ccatctggaa
     2821 tgggaagcta acctgagttt ctaactcttg ataaaagtca gcaacagaga ctttgcgacc
     2881 ccatagcgaa atcataccgc tttcggcttt acgatgaatg ccagataaat atagctcgcc
     2941 attttcgccc gatgttaaaa cacctctatc actactgtca taacggaaga aaacactccc
     3001 ttcttgacga agggtagtta tgactacatc tccttcttgc ccgaccacaa ggttactgtc
     3061 ttcatctaaa acctctacca gccgtttatc agtaacaagg tatttaacat cctcttcagc
     3121 ttcacctggt acataatcat acagaccaat cagccccgta tcattttgtg tatacgaaac
     3181 agttatatat acctcatcct caaaaatagt tgctaaatcg gcataccatt cttcaagcat
     3241 cttagctgtc acaggctcag aattaagttc cagcataatc tttttgcctg atagctgttg
     3301 cttcaactct ggtctagctg caagctgttc cattagctga taatgaaaag tcttataact
     3361 cataacgccc atttccataa aaatggcgtc gtgttctgca atagcttcaa tggcatcgtg
     3421 tgtttccttg aactcataaa caccaacatc aggtctaagt gcttgtgtcg tttctttggc
     3481 cacctgtgcg tctatacctc cccattcacc gtaaagaacc aatggttttt cagctgccaa
     3541 tataatcctg ccccattcgc caagcccaga agcatttacc cgctcttcgt ctgagaagta
     3601 aaactccttt tgagtgccag tagttccgcc agaataaagt tttatgagct tatctgtatc
     3661 acctgccgca aattgcttag gatcttctgc tgctcggcta ggcaatacta gaggaaaggt
     3721 ggtgacaaat tctgagtaag aaccaatgct ctctactgtc gcaaactttt gaccataatg
     3781 tgtcatcttt aaggcttgaa gcgtttgatt tactaattct tgcctggcct taaaagctaa
     3841 ttcatattgg cttatatcag gatttagttc ttgttctgaa ctagctgtta tctcggacat
     3901 atctggtaca agttcttgtg ttggcatttt tatttttagt gtatggttca atcctagcac
     3961 aaaagttcca agaagatcct gaagggtccc aaatgtttga aagcatactg tcagaaaaac
     4021 atttgtgtgt caatggtgcg ggtaacgaga ctctaactcg tgacctcttc cttggcaagg
     4081 aagcgctcta aacaactgag ctacacccgc tcgaatattg aaggacaacc tacggttttc
     4141 cttcaacgcg cctttggaat aaatccaatc cgccaaagcg gaccggcgct actacctttc
     4201 cctgaaacag ataaggacag ggtaaattgt agcaaaaacc cctgttaaaa gcaaagctat
     4261 ggtctattta tggaatggtt ggttgttgcg ggtttttagt ggaaatacca gtggccgaac
     4321 cggttgtggt agaacttgaa gaactggttg ttgtagtgct cgttaaagaa atggtcgttg
     4381 tggcaggtgc tgaaatcggt tgtcggaaaa gtatcgccgg cgtcaatgtg ccgcacaacg
     4441 gctctattgg gaccggtttg cgtgaatact ggtggggtag gaatgtagtt gtagtttcct
     4501 tggtcggcgt agccagtgcc ggagatgttg ttgttgcaag caaagtcctt gttgccgacg
     4561 tcattgattc cagcttgata gccaataaat ccacctccac aactcggatc gttgttacag
     4621 agcccgctaa cattagttac cgtgctgtcg gaaatccagt tatctacagc catatccttt
     4681 gtgggagtcg ttgcgggacc agtgccgcca gcgttgtaat tcaccatgaa tattccgacg
     4741 tcgttgttga caagtttgtt atctttaact ttaacgttag tgaccagcgg atcgcccaag
     4801 ccaccgaata gtaaaatgcc gcctgacgaa gcgaagttgg taccagtgta ggcgttgccg
     4861 gtaactttat tggatgtaat ctcaccggtg gcgccaaagc cgatttggat gccgttttgg
     4921 gcaatgtaat ctacggggcc ggcgccgtta acagtattct tatctacctt gcccttagcg
     4981 ttttggccat tgatgactat gccgcctttt tgatagttat caactaggtc atggttgatg
     5041 tccgcgctaa ctttaccgct ggtgttgagg ccgtagtaat agatgtcaat acctgtttga
     5101 acaccgttcg gcgagtagac tcccgacgtg tggttaccga cattgtaaac atggctgttg
     5161 gtcaccttaa ccgataagtt gccgctgatg ccgtctacaa acaccccgta tttattagcg
     5221 tcgtgaatcg aagctccgct gacatttccc gtatcagaac cattatagta cactccgatg
     5281 tcgcagccgc tggcgttaag atcaccagtt acatcacccc cgatttgtgc ggcagttaac
     5341 agtcctttcg aggtagttac aggatagcaa aatggagtcg ctgttcccgc aggcatcgag
     5401 aaggtttggc aattgttgtc aacggtccct gtgatcgagc cgtcgggagc aatcgtacca
     5461 ataccgtcta gtgtatagcc cgcgttagtg ccggtataga caatggtgaa agttaaatta
     5521 ttaccgctga cattgccgtt gatgttccat gtgtatgtgt tgtctggatt gtatgtgcct
     5581 gtcccagaga aactgccgga cgaatcctct gaaaccgtat taagcgtatg gttatagtcc
     5641 ccgcctccgc aaacaaaagt gatagcttct ggagcgttaa tctcccagag gctcggcgag
     5701 atagcatgcg ctgtcccgaa tagcgtcagc gataagccaa cacctccgcc aattagcgca
     5761 agagatgaaa cagctatcgc cagcttggtt cttgcctttg taatcccttt tttaaaatcg
     5821 tatttatatt tcatgatgga cactccttgc ccaccctttt tgaattgtac ttggagtata
     5881 gcacaataat attgacaaat aaaaaactgc ggctgctttg ctctttagcg tatctgttgt
     5941 aaaatacctc tgttatggcg ctttagctca gttggtagag cagaggcctg aagagccttg
     6001 tgtccccagt tcgagtctgg gaggcgccac cagaattgag cacgacctct tatttagaaa
     6061 tggcaaccag agctaatatt acggttgccg tttcttcatt tacggtcggc tcaattcttt
     6121 accccattgc ctctgagcca ggcaatgggg atcccaaata atcaattact ctacttcttt
     6181 tcgcgggcca ggaagttttt gatgtcttct acagtactca tgaactgggc tggaaagaca
     6241 acggtactgt tcttctcatc ggcaatctcc accaaagtct gcaagtttct aagctgcagg
     6301 gcgatcgggt gtttggagat gatgtcggcg gcttcgccca gcccggtggc gctcaatttc
     6361 tcgccctcgg cggcgataat cttggcccgc ttttcgcgct cggcctcggc ctgcctggcc
     6421 atggcccgct gcatgctgtc cggcagctga atatctttaa gctcaaccgt gctgacgtat
     6481 attccccact tggcggtggc ggtgtctaaa atctttttga ttttttcatt aatggctgcc
     6541 gtggcgctta acacctcgtc cagcgagctt tggccgacca cgttacgaac ggtcgtttgg
     6601 gcgatctggt aggtggcgtc cagcacattt tcaatttcca ccaccgattt tatggggtcg
     6661 ctaaccttgt agtaagccac cgccgccaca tctatagaaa cgttgtcttt ggtgatgatt
     6721 ttttggcttt caatcggcag ggtgatgatg cgcagattaa cgcggcgcat ctggtcgact
     6781 atcgggatga tgaaatttaa acccggctct ttgatgtccg ggcgcaattt gcccaatctt
     6841 agcactactc ctctttggta ctggttgact atccggattc cggccagggc gattagcaac
     6901 accagaacca gcacgactaa taagacgatc atgttgacct cctgatttta tggtgggatc
     6961 ggagggactt gaacccccga cacccgcagt gtaaatgcgg tgctctagcc agctgagcta
     7021 cgatcccttg gtgggcggaa gaggacttga acctccacag gatttctccc actaccacct
     7081 caaggtagcg cgtctaccgg ttccgccatc cgcccagaac agcgtaatta tacattaact
     7141 acaggtgtaa gtacaggggt gggtaaccta gatgtcttgt ttttgctggc ggacttccca
     7201 ctggttgaca ttgtaaaact ggtccagttc gccgttatcg gccttgcgct gataattgaa
     7261 taccgggccg tgggttatgt agagcaagtt aggttgataa agcgccaggg ctggaacatc
     7321 ctgaacccag gcagtcaaaa aggctttgta tttgagaact ctcacggctg ggtccgagcg
     7381 cgtgcggccg gcctccagcg cttggtcggc ggcggcggat ttatactcgc tcaagttcag
     7441 gtgcccttgc gagctgacgc tggcctgcga gctgtcccag taggcgaaaa cgtcggggtc
     7501 cacgccgatg ttgataccgt aaagcagggc gtcgtagtcg tggttggcta tgacgatgct
     7561 ttgcagatcg tcggcgtcgt aatactgcgc cgtaactttc acccccagtt tggcccactg
     7621 gcgctgtaga aactcagcca cccgggtgta ctgcggagta tcctgtgatt tcagcacgaa
     7681 gctcagcggc tggccgtttt tatagcggat accaccctgt cccaccgtcc agccggcttg
     7741 gtccagtgtt tgggaggccg ccgcgaagtt gtagctggcc tcgacgattg acgggtcgta
     7801 acccagctgg tttctgagca gcgggccgtc gaccggcttg accggtgtat caaacaaact
     7861 ttcaagttgc gcccgatcca cactttcctc cagagctttg cgaacgttag catcgcttag
     7921 caacggacgg gagttgttaa agaacgccat gacggcgctg gtcagcggcg tagtataagt
     7981 ttgcacgctg ctgtcgccgg ctaattcggc cggcacggac tccaggccgg taatggcatt
     8041 gagctgtttt tgcttaaaag cggctatgag gtggttatcg tcagaaaagg tgataatgct
     8101 aaaaccgtca agtttgggcg ccccggcgta gtaacggtcg taggccgcca gcgcgatacg
     8161 ctgctgacgg tcggcagtgg tgttaccggt gacattgatg aacttaagct caaacggtcc
     8221 ggtccctacc ggcgcggtgt taaaagcggc gctgcgcaat tgctccggcg gcaccgactt
     8281 taacagatga gccggtaaga tcccgttggt cagagcgtag gggaaagagc tcaaaggatt
     8341 tggcagagag aagttgacac tgtaggtgct ttgcttggtg accgtgatat ctttccaact
     8401 ggaaatgagc gaagattggg cgttggcatt ttggatggtg tggtaagtga agactacgtc
     8461 gtcggcggta aacggccggc catcctgcca gaaaatgttc tttttaagat gcaccacgta
     8521 gtctgtctgg ttggcgctga gcgtccagcc gttggccagg tcgccgacca actggttgtc
     8581 gtcgttatat ttaaagagcc cggagaacac cagccggctg acggccgtgt cggcggcacc
     8641 ggtggcgtaa ataggattgg cgttggtaaa gctgccgact aagccttcat tgtacaggcc
     8701 gcccggcacc ggctttaaaa cctggtaata tgccgatagg ttgcgcagct gggcgacgct
     8761 gatcaagatc agcagtacga acaggccgac ccaaagcagc acaaaccgcc ggacagagac
     8821 caagcggtca aagcgtctga taatcagttt ttctatgctg gcgtcggcct gctggcccag
     8881 ctggattgct ccgcggcgac ggcggttaaa acggcggcgc cagcgacgtt tggtatcatc
     8941 atccaattta ggaatagatg acttcacgca agctccggat tatttggcaa cgatgttgat
     9001 aatgatactg gcggcgaaga tgaagaccat gatgatagtc agctggtgca gggttttgtc
     9061 ggagccgcga cggacggtgt tgacttcgcc ggcgccgcct aaaccggcgc ccaagctggc
     9121 gccgcgggtc tgcaccaaaa tcaaaatcgt taaaataatc acgctgacga tggttatgta
     9181 attccaaatg gtcatgctct gctccgccgg ctgatgggct taattgtatc gtctaagaca
     9241 cgcgtcacta agaagtttac cagcccgaca atcaaaccgg caattatagc cacaccgaag
     9301 tctttaacgt aaagcgggtg gtacagccag ctggcggcca ggattaaaaa gccgttgact
     9361 accagcatga acaagccaag actgaccaaa atcgccggga tggacaggaa tactaggaac
     9421 ggcttgacaa acatgttgac cagcgccaag aagaaaccgg cggccagcac cgtcttccag
     9481 ctgccgccga cactcaggcg cgaactgccc aagatggcct cggctatcca tagccccagc
     9541 gaggacacca gccagcgcaa taaaaacttc attatcaccc gctgcaccct tacattgtac
     9601 caagaatcgc caccctgggg cgagcggctt tagcgcagcg ttttcagata tgcggcgatt
     9661 ttttggccgg tttggacgcc cagcagctct tgcagttcgg ctactgacgc gtccatgacg
     9721 cctttggccg agccaaagcg tcggatgagc tttttgcggg tcgccgggcc aacgccgggg
     9781 atttcgtcca gcaggctggc cgtgccgcgg cggcgcttga tggtactgtg gtaactgata
     9841 gcaaaacggt gtgattcgtc gcggatacgc tgcagcagct tgaccaacgg cgactgctcg
     9901 ggcagcttaa ggattgtgaa gcttttagcg gccggtaatt tcatgatgat ttcctcttcg
     9961 cgcttggcca ggccgatcat aggggtagtc agatcggcag cgtcgcgggc tttcagggcg
    10021 gccgccagct ggcctttgcc gccatctatc agaaacaaat ctggtaggcc ccatttttga
    10081 cgattggttt cagatagccg gcggctaagc gcttcgttga tgtgggcaaa atcgtcgtta
    10141 ccgggcagac gcattttgaa tttacggtaa gcggtctttt ccggcacgcc gtttagaaac
    10201 accaccatgc tggcaacggt atcggtgcct tgcaggtgcg aaacgtcaaa gccctctata
    10261 cggcgcggcg gcgccggtag accgagcagt tcggctaagc cgaccagtgc ctgatctttg
    10321 gacgcgtctt gcaattcatg gtcagaaaac aggatctgcc gcgataactg ccgcaacgcg
    10381 aacagctggt tgcgcagccg tgccgcctcc tcaaaatttt tagctttggc ggcctttttc
    10441 atgtcgcgct caatttcgcg gatcaaggcc acccgccggc ctcgcagata ttgcatcaac
    10501 ttgcgcaagt tggcacggta atcggccaaa ctggtccggc cttcttccaa accggggtcc
    10561 aaacccagat gataatgcag gctggcgcgt ttttggccgg ccggccggct gatggcatag
    10621 ggaaaagccc gccgcagata acgcagggct tttttgacgg cgaaaccgct gatgtacggg
    10681 ccaaaatagc gcgcgccgtc atccaacgga cgccggacca tggctactgt cggatagtcg
    10741 ctgccgtagt caatccgtac gtactgcaaa ctcttatcat cgcgcagcag aatgttaaag
    10801 cgcggctggt aacgccgtac cagctcggct tccaaaaaca gggcgtcggc ttcattctcc
    10861 agctcggtcc agtctaaatc ggtgatctcg gttaccaggg catcggtttt cgggtcgcgg
    10921 gcccgacttt tttgaaaata ctgacgcacc cggttgcgca aattcgccgc cttgccgatg
    10981 taaataatct cgccagagtt atctttataa aaatatacgc ccggcgaggt tggcaagttc
    11041 ttaagtttgg actccgcccg tttattcata catgaataat tataggttat gcatctgggt
    11101 gcagtgcaag aaagaagcga gcactggaac gagctgtatt taacatacgg tgagtgaagc
    11161 gcgcagtttc tgacgccgca atgcgccaga agcgcccggc aggtttcggc tatgccgaaa
    11221 gcaagccggg cgcggtgcat aagctataaa aatttgtcat cagatcggca taagtgttat
    11281 aattgccaat cttagtaatt aacagggaga aaggagcagc catcatgaag aaaaagacac
    11341 agaaaacagt taatacagct gctccggcag cctgttaact ctctcagata aacatcatgt
    11401 ttgaatttaa ttaaggtaaa cgcggccagg agaccgcgac catgtccacg accagaaaaa
    11461 ccctatcaat ttactggcag catactcttc ggtatcgctg gctttttgcc gccggaacgt
    11521 ctggcgcggc ggctgctgta attgtccagg acattgtccc gccgtttatt gtcgctaaag
    11581 cctttaacac cctgcaatcc gtctacagca gcggcggtca actaaaattt tccgaactgt
    11641 cgggatatct tattgcctat cttattttta tgcttggcgg tgtgatcttg tggcgcgcac
    11701 aggtgatttg tgtctggctt tatgaaatga aagttttgcg cgacatcgct aaccgggttt
    11761 ttgattttct gcagcaccag agctaccgct ttcacgccaa ccactttgcc ggcgctttgg
    11821 taacccaggc caaccgtttc atcaactctt acgagtcgct gatggacagt tttatttggg
    11881 atatcaccag cggcgctaca acactagtcg cttcaattat ttttctttta ttcgtatcca
    11941 aggtttactc tctgatttta ctggctattt ctttagtcta cttgagcata atctaccacc
    12001 gggcccgtgg ccagataccg tataaccgtc gcgagtcggc ccgccaaagt gaccaaaccg
    12061 cggccctggc cgacgtaatc actaacgttg gcacggtttc cacctttgcg gctgaaaaac
    12121 gcgaatacca gcgcttcgca aaagttaccg aagctaaata cagcgcctct cacgccctga
    12181 tggtgtacgg gatgaaaacc gagattatga gccacttcat gaccgcgtcc attaacatta
    12241 ccgccttagt tacaggagtt ttggctatta cgaccttcca tgccagagtc agcgttttgg
    12301 tcttggctgt ttcgtatacc cagggcatca ccggccgctt gtggcagttt ggtcgcgttg
    12361 tgcgcaacat caataaagcc ctgggcgacg ccgcagaaat gacggagatg ctgcagcagg
    12421 aaccggaaat taaagaaccc gcttcgccta agaaactgcg gattgaaaac ggctcaatca
    12481 attttgacca cgtggagttc ggctacgccg acggccaggg acaagtcgta ctgccaaact
    12541 taagtttaag tgtaccgggc ggcaaaaaag ttggtttagt cgggtattcc ggtggtggca
    12601 aaactaccat ctccaagctg gtcctgagat taatggacat ccaaaaaggc agcttgacga
    12661 ttgatgggac cgacatccgt gaagtcgcct tggcggatct gcgcacaaaa atcgcttacg
    12721 tgccccagga acccctgctt tttcaccgga caatcagcga aaatatcggc tacgctaaac
    12781 cggacgccag catggcggaa atagtccagg ccgccaagat ggcctatgcc cacgaattca
    12841 tcaaagacct acccgatggt tacgacactt tggttggcga gcgcggtatt aagctttccg
    12901 gcggccaacg ccagcggatc gccatcgccc gggcgatgct gaaaaacgcg ccgatcttag
    12961 tgctggatga agccaccagc gcctt
//
LOCUS       DASPTF010000086        10400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_1006172, whole genome shotgun sequence.
ACCESSION   DASPTF010000086 DASPTF010000000
VERSION     DASPTF010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10400)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10400
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006172"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VEH48_02910"
     CDS             <1..854
                     /locus_tag="VEH48_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18340.1"
                     /translation="EKAILTKLKMAAHNDLAVIKDVEKALGQHLESVGKVARVSDVAA
                     KLTPSDSKVDQARLSFLAQLSPAITVVEDSDNFFHSLALAAEHSEKAINQEVDKIIEA
                     VNKIGEPTDINTLAKRLGAKDAKNVEALASISKNLAQLHGRWGLIKWPMVNPKNIRDK
                     IYVILHDAKKPMHFSEIAAAIKKSDFKRKDVTTQAIHNELIKDKRFVLIGRGIYALRE
                     WGYKKGTVADVIAEVLRKEGGPLHRDEIVRRVLKSRQVKETTILLNLQGKPQFKRVAK
                     ATYTLAE"
     gene            962..3616
                     /locus_tag="VEH48_02915"
     CDS             962..3616
                     /locus_tag="VEH48_02915"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HXY18341.1"
                     /translation="MALFARAKGARAAHEHCLLMLEIPRTNDKKELAAEQMLAALHGI
                     LRSKRDLKLSGTMQEHFSLEIASIGQRLRFYIWTPKHLQAFVEGQIYAQYPTVQIYEQ
                     DEDYAGRNLSQSVIHSGELVLADNETLPIKTFPSFEVDPLAAITATLAKLDGQDEEIW
                     IQIIARPVSDDWHRKGARMVERIKRGGGLFGGGAGGSTLSYASQVFGALSKPPETGVG
                     GESKAPELSERDKTRVTAIQEKGTKLGYQVKIRLLYAGHDQYTARLRMQALVGAFKQF
                     NTTNLNGFEAKKATFNRDVQLEYKERFFIDKGFILNIEELASIFHLPHTTVETPNIVW
                     ANTKTAEPPSNVPTLATSTDKDISLFGVTNFRGDNTVFGILRGDRGRHVYILGQTGTG
                     KSGALELFTLSDIYYDQGFAVIDPHGDYAQHVLKFIPERRIDDVVYFNPADTDFPIGF
                     NPLEISDPTLKGHISSELVGVLKRLFAESWGPRLEYILRYTLLALLDYPESTMLDITR
                     MLTDKKFRAGVITHIDDPVVRNFWVTEFAAWNDKFATEAVAPVLNKVGAFTANPMIRN
                     IIGQPKSTFNLRQIMDQGKILIVNLSRGLMGEDNAGILGAMMVTKIQLAAMGRADMPE
                     AERRPFYLYVDEFQNFATESFATILSEARKYALNLTVANQYILQMEEPVRNAVFGNVG
                     TIVCFRVSPDDAPFLVKYFEPQFEPGDLIQQHNRYFVITMTIDGEKAPAFSAKTLNLP
                     EPPADATRRIVELSRERYTQDRATVDAFIRKNSGLEPPATPSQPAPQPERVKAQVQLN
                     ELAKQESQHQSNSGTKPPADTGSFVSGFGGSGEEPRRKRTRRGGRKHKERQQQAQQAA
                     SEAAKKPDTPVKPGQKPVSSDEYVIHLR"
     gene            complement(3623..4294)
                     /locus_tag="VEH48_02920"
     CDS             complement(3623..4294)
                     /locus_tag="VEH48_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18342.1"
                     /translation="MDSKLRIARLGNQDGKWTRSQISAGLQKFYEVYGRYPTSREIDA
                     FEYLPSARSIQRKYGGLISIRKELIPESHADYTKGAYRSAKAKESWHRAAKYEEEFYN
                     FLLEHFDPVAVHEHKIMRPGNIASDFYIYTSESDGVVIDLFYAQDLFSLSSIVSIKLK
                     RYVGLPYRIIFVLVGNQTITAQRIQKLMLNRISSLPKNISIDTEANFKSATIFAIKRN
                     SKYSI"
     gene            complement(4909..5526)
                     /locus_tag="VEH48_02925"
     CDS             complement(4909..5526)
                     /locus_tag="VEH48_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXY18343.1"
                     /translation="MEKTNLDEVTNLFTELVFPFYEVNRDMIAPLSERRRENDAEHSW
                     ALGLIACALAERIDTSLDVGKIAQLAAVHDLVEIGAGDTSVWDESMKATKEEREKQAL
                     IELKRKFSTFPWLVNNLEEYETRLTPEAKFVSAVDKYIAVVTRLLDARAGNRVYINEV
                     PLTVKQYIAGIKAARHKAHAHQGVGEMYEEVYSIFLSHPEWFVEE"
     gene            complement(5587..6468)
                     /locus_tag="VEH48_02930"
     CDS             complement(5587..6468)
                     /locus_tag="VEH48_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18344.1"
                     /translation="MSARIAKSDQPRRSAARDKLAPPAKYYRSGQLSDKAASPFKSQA
                     KPPKAARRKVLFGLADIIVVTALVVGLAYSLLVKPDPEILANSSAYHPSSVYKQAAIK
                     QFQLLKNRNKVSLDETGIVQSLERQFPEIAGASVELPLFGETPILHLDIAPPSFFLAS
                     AGKSYIVDQQGKVVAQSASLPEIKNLTEVIDQSGFTVIIGSQTLSASDVNFINTVAAE
                     VNYAKIPIASLTLPSLAQEMDLRTTDCGYYVKFFLGGDPLLQSGQFLAARYNFDQSGS
                     QPSQYLDVRVPGKIYYK"
     gene            complement(6465..7685)
                     /locus_tag="VEH48_02935"
     CDS             complement(6465..7685)
                     /locus_tag="VEH48_02935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXY18345.1"
                     /translation="MSANPAKTPRHKPAPVIILTGGGSGGHITPLLSLAHELKAQRPS
                     CKIIYIGLKGEKVEGLQNQYQVFDQVYHIAAGKFRRYYGQSFLAHLGDVKTQALNSRD
                     FFRVIKGLHSAEKIVKAVKPDVVFSKGGYVAVPVGLAAKLNAVPIVTHDSDSVPGLAN
                     RLAGRWAVVHATGMPVEYYNYGRSKTVYTGVPVDKRVRPLSTSLQRSYKKKLGIPADS
                     LVLLAAGGGQGAQQINDLMLAAAPKLLAQFPKLYILHFAGIKLAGQMGVERVRELYHR
                     LLSDSKRVQVLDFSGEFYKYSGAADLIITRAGATVMSEFAVQGKACIVIPSAVLAGGH
                     QLKNSQVLAAQKAAVVLPADVTAGRLLKLTSNLLKSPAKRKLLAKNLGSTAQPQAARK
                     LAALIIEVAEKSLP"
     gene            complement(7570..8865)
                     /locus_tag="VEH48_02940"
     CDS             complement(7570..8865)
                     /locus_tag="VEH48_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HXY18346.1"
                     /translation="MDRRPRHSPSRALMNAQADHRTAVGLLGRKHKPDYWILILASLL
                     VTVGLVVVYSISPGLAASQNISQNYYITKQLIDVGLGIGIFALAAYIPLGNWHKLGRP
                     LAIAALIGCLVVMITPLNAVYPAHRWIRLGSFSFQIAELIKLSLIIWLAGFLSRQWRA
                     GKLADFNSTLKPLLFLILGLGIVVAKLQSDFGSAAVMVAIIILMAYVAGIPLKKTAII
                     LSVIVALLSILIISTPYRRERITAFLHPQTNCQGSGYQACQDLISVGSGGVLGLGLGY
                     SVQAYGYTPEASNDSIFAIIAEEVGFVGSMIIVVIYGVFISRLKRVIEHSADQFNRLV
                     TVGVLAWLSTQMIINIGAMVGLLPLKGITLPLISQGGTSLVFLAAALGLVFQISRYTS
                     YDVSEPSQNSASQAGAGNYPNRWGVRRPHHAPLVARPRT"
     gene            complement(8870..9907)
                     /gene="mraY"
                     /locus_tag="VEH48_02945"
     CDS             complement(8870..9907)
                     /gene="mraY"
                     /locus_tag="VEH48_02945"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXY18347.1"
                     /translation="MSSLQLVVNSDNIEQIFTFGFLSFVTAMVLTPLYTTAAFSGKWW
                     KRPRTKAITGEKAKVFQKLHAAKHQRHIPTMAGIIFILATALVTLAGNLSRSQTWLPL
                     AAMVGAGGIGLLDDIINIRGVGGGIAGMRVKVKALLLIAIALIGGWWFYNKLGVSTIH
                     IPFFDTWHIGWWIIPLFILVVFTTANAVNMTDGLDGLAGGLCTIAFGAYAVISLIERK
                     YGLAGFCMTVAGALLSYTWFNIYPARFFMGDVGSFALGTALAVVAMLTDTVLLLPVIG
                     AVFVAEGGSVALQILSKKLRGGKKIFLSSPIHHHFEAIGWPETKVTMRFWVIGEVAAF
                     VGVILFILGGY"
     gene            complement(9957..>10400)
                     /locus_tag="VEH48_02950"
     CDS             complement(9957..>10400)
                     /locus_tag="VEH48_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXY18348.1"
                     /translation="GGTYYQPNLVAQTISADGKVTATAPKVVRTNVVSAKIAPELEGL
                     MEYVVQGHLADGFTYLKFPANYMVGGKTGTAQIADPAGGYYSDKFNGTYVGFVGGDKP
                     QYVIIVRANTPGISGYAGSVAAQPIFASLAHMLIDNFNVTPTSGS"
BASE COUNT         2562 a         2716 c         2663 g         2459 t
ORIGIN      
        1 tggaaaaagc cattttgacc aagctcaaga tggccgctca caatgactta gctgtaatca
       61 aagacgttga gaaagctttg ggccagcact tagagtctgt cggtaaagtt gcccgcgtca
      121 gcgatgtagc cgccaagttg actccctccg attccaaagt ggaccaagcc cgtttatcat
      181 ttttggccca attgagccca gctatcaccg tggtggaaga cagcgataat tttttccact
      241 ccttggcctt ggctgccgaa cactccgaga aagccatcaa ccaggaagtc gataagatta
      301 tcgaggccgt caacaaaatc ggcgaaccta cagacatcaa cactctggct aagagactcg
      361 gcgccaaaga cgccaaaaat gtcgaagccc tggccagcat ttcaaagaac ctggcgcagc
      421 tgcacggccg ctggggcctg ataaaatggc cgatggtcaa ccccaagaat atccgcgaca
      481 agatctatgt catactgcac gacgccaaaa agccgatgca cttcagcgaa atcgccgcgg
      541 ctatcaaaaa aagtgatttc aagcgcaaag atgtgaccac ccaagccatc cacaacgagt
      601 taatcaaaga caagcgattc gttttaatcg gacgggggat ttacgccctg cgcgaatggg
      661 gctacaaaaa gggcactgta gccgatgtta tcgccgaggt actgcgcaaa gagggcggtc
      721 cactgcaccg cgacgagatt gtccgccggg tgctcaaaag ccgccaagtc aaagagacta
      781 ccattttgct taatttgcag ggtaaaccgc agttcaagcg ggtcgcaaaa gccacttata
      841 ccctagccga ataaggtcag atttgtagtc gtgttgggct aaagggttat agaaactaca
      901 cacctacacc cctataaccc tatgcttaaa gatgtataat agataagagg tatacctctt
      961 tatggcgtta tttgctagag caaaaggtgc aagggcggcg catgagcatt gtctgctaat
     1021 gctggaaatt ccgcgcacca acgataaaaa agaactggcc gccgaacaga tgctggcggc
     1081 tctgcatggt attctgcgct ctaagcgcga cctcaagctt tccggcacga tgcaagaaca
     1141 cttcagcctg gagatcgcct ccatcggtca gcggctacgt ttttacattt ggacgcctaa
     1201 gcacttgcag gcttttgtgg aaggccaaat ttacgcccaa taccccacgg tacagattta
     1261 tgagcaggat gaagattatg ccggccgcaa cttaagccag agcgtcatcc acagcggcga
     1321 gctggtactg gccgataacg agaccttacc tatcaagact tttcccagtt ttgaggtcga
     1381 cccgctggcg gccatcaccg cgaccctggc caaattggac ggccaggacg aggaaatttg
     1441 gattcagatt atcgcccggc cggtatcgga cgactggcac cgcaaaggcg cgcggatggt
     1501 ggaacgtatc aaacgcggcg gcggcttgtt cggcggcgga gcagggggca gtaccctcag
     1561 ctatgccagc caggtattcg gtgctctttc caaaccgccg gaaaccggcg tggggggcga
     1621 gagtaaggca ccggaacttt cggaacgcga taaaacgcgg gttacagcca tacaagagaa
     1681 ggggactaag ctgggttacc aggttaagat ccgtctgctt tacgccggcc acgaccagta
     1741 caccgcccga ctgcgcatgc aggcgctggt cggcgctttc aagcagttca atactaccaa
     1801 cttaaacggt tttgaggcca aaaaagccac ctttaaccgc gatgtccaac tggagtacaa
     1861 ggagcgcttt ttcattgata agggttttat cctgaacatt gaggaattgg ctagtatttt
     1921 ccatctgccg cacaccactg tggagacgcc caatatcgtc tgggccaaca ccaagaccgc
     1981 cgagccgccg tctaacgtgc cgaccctggc aacttccacg gacaaagata tcagtctgtt
     2041 cggcgttact aactttcgcg gagataacac cgtctttggc atactgcgcg gtgaccgcgg
     2101 ccggcatgtc tacattttgg gccagaccgg caccggcaaa tcgggcgcct tggagctatt
     2161 cacgctgtca gacatctatt atgaccaggg cttcgcagtc atcgatccgc acggggatta
     2221 tgcccagcac gtcctgaaat tcattcccga gcgccggata gatgacgtgg tttacttcaa
     2281 cccggccgat accgactttc ctatcggctt taacccgctg gaaatttccg acccgacact
     2341 caaaggccac atcagctccg agctagtcgg agtgctgaaa cgtttgttcg ctgaaagctg
     2401 gggaccgcga ttggaatata ttctgcgcta caccttactg gcactgctgg attatccgga
     2461 atccactatg ctggatatca ctcgcatgct gaccgacaaa aaattccggg ccggtgtaat
     2521 cacgcacata gacgacccgg tggtgcgcaa cttttgggtg actgaattcg ccgcctggaa
     2581 cgataagttc gccactgaag ccgtggcgcc agttttgaac aaggttggcg catttaccgc
     2641 taatccgatg atcagaaaca tcattggcca gcccaagagt acctttaacc tacgccagat
     2701 catggaccag ggtaagatcc tgatagtcaa tcttagccgc gggctgatgg gtgaagacaa
     2761 cgccggtatt ttgggcgcca tgatggtcac aaaaatccag ctggcggcca tgggccgggc
     2821 cgatatgcct gaagccgagc gccggccgtt ttatctttac gtggacgaat ttcagaactt
     2881 cgccaccgag agctttgcca cgattttgag cgaggctcgc aaatacgcct tgaatctgac
     2941 tgtcgccaac caatacattc tgcagatgga ggaaccggtc cgcaacgctg tttttggcaa
     3001 tgtcggcaca atcgtttgtt tcagggtaag ccccgatgac gcgccgttcc tggttaaata
     3061 ttttgagccg cagtttgagc ctggtgacct tatacaacag cacaaccgct attttgtcat
     3121 cactatgacc attgacggcg aaaaagcacc tgcgttctcg gccaaaaccc tgaacctgcc
     3181 cgagccgccg gctgatgcca ccaggcggat tgtggaactt tcgcgtgagc gctacaccca
     3241 agaccgtgca accgtagacg cctttatccg gaagaattcc ggcctagagc cgccggctac
     3301 tcccagccaa ccggctccgc agccggagcg cgttaaagcc caggtgcagc tgaatgaatt
     3361 agccaagcaa gaatcacagc accaatctaa ctccggtact aagccgccgg ccgataccgg
     3421 tagttttgtt tccgggtttg gcggtagcgg cgaggaaccc agacgcaagc gcacccgccg
     3481 cggcggacgc aagcacaagg aacgccagca gcaagcccag caggcagctt cagaggccgc
     3541 taaaaagcct gatacgcccg tgaagcctgg tcagaagccc gtatccagtg atgagtacgt
     3601 catccacctg cgctaaacct atctagatcg agtacttaga gttcctcttt atcgcaaata
     3661 tcgttgccga tttgaagttg gcttcagtat caatggatat gttttttggt aatgagctta
     3721 ttctattcaa cattagtttc tgaattcgtt gggcggttat tgtttgattg cctaccagaa
     3781 caaatattat tctgtacggc aggccaacgt accgcttcag cttgattgag actatgctcg
     3841 aaagtgagaa taaatcttgg gcataaaata agtcgatgac aaccccgtcg ctctcagaag
     3901 tatatatgta gaagtcgcta gcgatattgc ctggtctcat aatcttgtgt tcgtgcactg
     3961 caacggggtc aaagtgttca agtagaaagt tataaaattc ttcttcgtat ttcgcggctc
     4021 gatgccatga ttctttagcc ttagcgcttc gatacgctcc ttttgtgtaa tctgcatggc
     4081 tttccggtat taattcttta cgtatagata tcaagccacc atatttacgt tggattgatc
     4141 gtgcacttgg taaatactcg aatgcgtcga tttcgcggga agtggggtat cttccataaa
     4201 cttcgtaaaa cttctgtaat ccggcggata tttgcgatcg cgtccatttg ccatcctgat
     4261 taccaagtct ggcaattctc aacttgctat ccataaatca ataatatcat ttaattaaag
     4321 aacattttgc gaacacgatt agccgcaggc tggttgggtg atagtcatga ctgactgtcg
     4381 cagagcattg agttagtgga gcatatataa taattaaata aaaaatgctg gcagcagaaa
     4441 accgtgtcag ccagaggcaa agggcaatat gttagtagag atatacatga tgtcgtacaa
     4501 tgtacctttc acgacgtccc gtttgttctt tggagttgat aacttatacc tcccctaacg
     4561 ggggagtttt aagcgctggc gtataaatga caagtttgcc cgtactgctc cacacaatta
     4621 tccatttatt aatgctttat ctgccttatc ccgtggtgtc gtaaaatttc cgtgaaaagt
     4681 gcccaagtta tccacagcca tatttgacta tttgactttt cacactttcg tattttcgta
     4741 tagtttcccc tacttttcct gtcaattatt tttcatttga cttttgacta aatagtatcg
     4801 gaatatactt ttccctgcac caaattaatt tttacatcta ctgaacaaat tacgaactag
     4861 agtggggcat tagaccacct tcgcgttgta gctaacttct ttacggtatt attcttcgac
     4921 aaaccattct ggatgtgaaa gaaatatgga gtagacttct tcatacatct cacctacccc
     4981 ctgatgtgcg tgggccttat gccgcgccgc tttaatacct gcaatatact gtttcacggt
     5041 taggggaact tcatttatat aaacccggtt gccagcccgg gcatcaagga gtcgtgtaac
     5101 aacagctatg tatttatcta ctgccgagac aaatttggct tcgggcgtaa gtctagtctc
     5161 atattcttcg agattattaa ccaaccaagg gaaggtgctg aactttctct taagctcaat
     5221 tagcgcttgt ttttctcttt cctctttggt ggctttcatg gattcatccc atacagaggt
     5281 atcccccgcg ccaatttcta ccaaatcgtg cacagctgct aactgggcta ttttcccaac
     5341 atccaagctg gtgtctatcc gttcagctag agcacaagca atcaacccga gcgcccagct
     5401 atgttcggca tcattctctc ggcggcgttc agataaagga gctatcatgt ctctgttaac
     5461 ctcataaaat ggaaatacta gctcggtaaa taggtttgtc acttcatcaa gattcgtttt
     5521 ttccatttct aaagaatatc aatttaaaac aaatatataa aatttacaac ggtttggttc
     5581 cctgctctac ttataataga ttttgccggg gacgcggacg tccaggtatt gtgatggttg
     5641 gctgccggac tggtcaaagt tgtagcgggc ggccaggaat tggccggatt gcaatagcgg
     5701 gtcgccgcca aggaaaaact tgacatagta gccgcagtcg gtagtacgca gatccatctc
     5761 ctgtgccagc gaaggcaaag ttaaagaggc tatcggtatt ttggcgtaat tgacttcagc
     5821 cgccacagta tttataaagt ttacgtcgct agcgctaaga gtctggctgc cgattatcac
     5881 cgtaaagccg ctttggtcaa tgacctccgt caagttctta atctccggca agctggcaga
     5941 ttgggccact accttgccct gctggtctac aatgtacgat ttaccggcgc tggccagaaa
     6001 aaaactcggc ggcgcgatgt ccaaatgcaa aatcggcgtc tcaccgaaaa gcggtaactc
     6061 cactgaggca ccggcgatct cgggaaactg cctctccaga ctttggacga tgccggtctc
     6121 atccagtgag actttattac ggttttttag cagttggaac tgctttatgg ctgcctgctt
     6181 atataccgat gacgggtggt aggcgctgct gttggctagg atttccgggt caggtttgac
     6241 cagcaggcta taggccagcc ctaccactag agcggtaacg acaattatat cggccaggcc
     6301 gaacagaact ttacgcctag cggcttttgg cggcttggct tgggatttga atgggctggc
     6361 ggctttgtcg ctgagctggc cggagcgata gtatttggcc ggcggcgcta gtttatctcg
     6421 agcggctgag cgtcgcggct ggtcagattt tgctattctg gcgctcaagg caaacttttc
     6481 tccgcgactt caataattag agcggctagt ttcctggcgg cttggggctg ggcggttgaa
     6541 ccaagatttt tcgccaacaa tttacgcttg gcgggcgatt ttaacagatt tgaggtcagt
     6601 ttgagtaagc gcccggcggt cacatctgcc ggcaacacca cggcggcttt ttgcgcggct
     6661 aggacctggg aatttttgag ctgatgaccg ccggccaaga cggcgctggg tatgacgatg
     6721 caggccttgc cctgaaccgc gaattcgctc atgacggtag cgccggcacg ggtgataatc
     6781 aggtcggccg cgccgctgta cttataaaac tcgccgctga agtccaggac ctggactctt
     6841 ttactgtcgc tcagaagacg atgataaagt tccctcaccc gttccacgcc catttgtccg
     6901 gccagtttga tgccggcaaa atgcaggatg tacagttttg ggaactgggc aagcagtttt
     6961 ggcgcggcgg ccagcattaa atcgttgatc tgctgggcgc cctggccgcc gccagcggct
     7021 aataacacca ggctgtcagc tggtatgcca agttttttct tgtaagaccg ctgcagactc
     7081 gtgctgagtg gccggacgcg tttgtccacc ggtacgccgg tatagactgt cttgctgcgg
     7141 ccgtagttat aatactctac cggcataccg gtggcgtgca ccaccgccca gcggccagcc
     7201 agccggttgg ccagcccagg tacgctgtca gagtcgtgcg tcacgatagg cacggcattt
     7261 aacttggcgg ccaggccaac cggcaccgcc acgtaaccgc ctttagaaaa cactacgtcc
     7321 ggtttaacag ccttaacgat tttttcagcg ctgtgcaggc cctttatgac cctgaagaaa
     7381 tcacggctgt ttagcgcctg ggtcttaacg tcgcctaaat gcgccaaaaa gctttggcca
     7441 tagtaacggc gaaacttacc ggcggcgata tgataaacct ggtcaaatac ctggtactgg
     7501 ttttgtaagc cttcaacttt ttcgcctttc agtccaatgt aaatgatctt gcatgagggt
     7561 cgttgggcct taagttcgtg ggcgagcgac aagaggggcg tgatgtggcc gcctgacccc
     7621 ccaccggtta ggataattac cggcgccggc ttgtgacgcg gagttttggc tgggttcgct
     7681 gacatcataa ctagtatagc gtgaaatctg gaaaactaag cccagcgcgg cagccaagaa
     7741 taccagactg gtacccccct gactaatcag cggcaaggtt atgcctttga gcggcagtag
     7801 gccgaccata gcgccgatgt tgataatcat ctgggtgctt agccaggcca gcacgccaac
     7861 ggtcaccagg cgattgaact ggtcggcact gtgttctatc acccgcttga ggcgcgagat
     7921 aaaaacaccg taaataacta cgataatcat tgagcccaca aaaccgactt cctcggcgat
     7981 tatcgcgaag atggagtcgt tggaggcctc aggcgtatag ccgtaggcct ggacgctgta
     8041 gcccagaccc aggcccagta caccgccgga acccacagaa atcaaatcct gacaagcctg
     8101 gtagccgctt ccctgacagt tagtctgggg gtgtaaaaag gccgtgatgc gttcgcgccg
     8161 gtacggcgta gagatgatta aaatagataa cagggccaca atgacactaa gaataatcgc
     8221 ggtctttttc agcgggatgc cggccacata agccatcaat atgattatgg cgaccatgac
     8281 agcggccgag ccgaaatcgc tctgcagctt ggcgaccacg ataccaaggc ccaatattaa
     8341 gaacaatagc ggcttaagcg tagaattaaa atcggccagt ttccctgccc gccattgacg
     8401 acttaagaaa ccggccagcc agatgataag cgatagctta atcagttcgg caatctggaa
     8461 cgaaaaactg cccaggcgga tccagcgatg agccggatat acggcgttta aaggtgttat
     8521 catcaccact aaacagccga ttaatgcggc gatggccagc ggtctgccca gtttatgcca
     8581 attgccaaga ggtatataag ccgccagcgc gaagattccg atgcccaaac cgacatctat
     8641 cagctgcttg gttatgtagt agttttggct gatgttctgg ctggcggcca gtccggggct
     8701 gatagaatag actaccacca ggccgacggt caccaacagc gacgctaaaa tcagtatcca
     8761 gtaatcgggc ttgtgcttac ggccaagcag ccccacagct gtgcggtgat cagcctgggc
     8821 gttcatcaaa gcccggctgg gactgtgacg cggccggcgg tccataaagc taataacccc
     8881 cgaggatgaa taggattacg ccgacaaatg cggcaacttc gccgattacc caaaaacgca
     8941 tggtgacctt ggtctccggc cagccgatgg cttcaaaatg atggtggata ggagaagaca
     9001 gaaagatttt cttacctccc cggagttttt tgctaagtat ctgtagggct accgagccgc
     9061 cttcggccac gaataccgcg ccgattaccg gcagcagcag caccgtatcg gtcagcatgg
     9121 ccaccaccgc cagagccgtg cctagagcga acgaaccgac atcgcccatg aaaaagcgcg
     9181 ccggatagat attaaaccag gtgtaactaa gcagcgctcc ggcaacagtc atgcaaaaac
     9241 cggccaggcc gtactttctt tcaatcagag atatcacggc gtaggcgccg aaagcgatgg
     9301 tacacaagcc gccggccaga ccatccagac cgtcggtcat attgacggcg ttggccgtag
     9361 tgaagactac caatataaac agcggaatta tccaccagcc gatgtgccaa gtatcaaaaa
     9421 acgggatatg gatggtgctg acgcccagtt tgttatagaa ccaccagccg ccaatcaagg
     9481 cgatggcgat caacagcagg gctttgactt tgacccgcat cccggcgata ccgccgccga
     9541 cgccgcggat attgattatg tcgtcaagca agccgatgcc acccgccccg accatggccg
     9601 ccagcggcag ccaggtttgc gagcggctga gattgcctgc caaagttact agagctgtcg
     9661 ccaggataaa aatgatgccg gccatggtcg gtatgtgccg ctggtgcttg gcggcgtgca
     9721 gtttttggaa aactttagcc ttttccccgg ttatcgcttt ggttctagga cgtttccacc
     9781 atttacccga aaaagcggcc gtggtatata gcggcgtcag caccatggcg gtaacaaagc
     9841 tcaggaaacc aaaagtaaaa atttgttcaa tattgtcgga attgactact agctgcaagc
     9901 tgctcatttt gtatttttca tttggctggt aagcattata ccacttttgc ggtcagtcaa
     9961 gagccgctcg tgggtgtgac attaaagtta tcaatcagca tgtgcgccag gctggcgaaa
    10021 atcggctggg cggcgacact gccggcatag ccgctgatgc cgggcgtgtt agcccgcacg
    10081 ataatcacat actgcggctt gtcgccgccg acgaagccga cataagtgcc gttgaacttg
    10141 tcgctgtagt agccgccggc cgggtcggcg atctgagcgg taccggtttt accgccgacc
    10201 atgtaattgg ccgggaattt caagtacgta aagccgtcgg ccaggtgtcc ttggacgaca
    10261 tattccatca ggccttccag ctccggggcg attttggcag ataccacgtt ggtcctgacc
    10321 actttgggcg cggtggctgt gactttgccg tcggcgctga tggtctgggc taccaaattc
    10381 ggctgataat aagtaccgcc
//
LOCUS       DASPTF010000087         8879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosopolaris sp. isolate SMAG_U12270
            k141_2154450, whole genome shotgun sequence.
ACCESSION   DASPTF010000087 DASPTF010000000
VERSION     DASPTF010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945186
            Sequence Read Archive: SRR7004338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosopolaris sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosopolaris sp.
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae; Candidatus Nitrosopolaris.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8879)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 01/09/2024 03:45:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 599
            CDSs (total)                 :: 562
            Genes (coding)               :: 562
            CDSs (with protein)          :: 562
            Genes (RNA)                  :: 37
            rRNAs                        :: 1, 1 (5S, 23S)
            complete rRNAs               :: 1 (5S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 35
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Candidatus Nitrosopolaris sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2154450"
                     /isolate="SMAG_U12270"
                     /isolation_source="soil"
                     /db_xref="taxon:3069383"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VEH48_02955"
     CDS             <1..518
                     /locus_tag="VEH48_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18349.1"
                     /translation="EPFLSMTVNTSSVNLGNLSTSTTATGIATFDVTAYVDSGYTVQT
                     VSQPPSYTSGSSSHTLTAMSLGGSVVGTEQYGINLVVNTSPASFGSNPVPEPSSAFAN
                     GAAATGYNTPNEYAYNAGDVIACAGTSGSCTGSGWGDTTFTISYIANISLVTPAGNYS
                     AIQDLVVVATY"
     gene            724..2010
                     /locus_tag="VEH48_02960"
     CDS             724..2010
                     /locus_tag="VEH48_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18350.1"
                     /translation="MWLVPFVVPNITDAQQLTLRSLTMSSGVPSNSGVNNTYTFGFTT
                     GSSATGTLQGMKLVACQTAVQTYGFASSASTTGCVAPTGLTFASAVWVSQTGILPTGS
                     PVNFAVDGTGGGFCTPAANVLCAKRSSATTQTASLAITIKFSTITNPSLAGGNCSANG
                     CSFYIGIYTYQTSYASPIDSGTVAGAVVQTLQVTAVVAEILQFCVGSTSVDDATTAVG
                     NASSCSGTTVDLGTLNPTAINYPITNGTSPNNVNGVAILRTNAINGSSVNYDSIQDTD
                     TGSGQHGDLKIQGTNCVGGVTPNPNACITSKASQGVFTAGTSDFGMTIGGTNCGTETA
                     GGYYTCSYSGGSEHLVPQTGYIGWASNYCGEATCNAHNGFKWDDTGTSDTLAVAASNN
                     VVADEALILQFAATPSITNTFGPYSVKVDFTALPTF"
     gene            2086..3102
                     /locus_tag="VEH48_02965"
     CDS             2086..3102
                     /locus_tag="VEH48_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18351.1"
                     /translation="MLIFIGLWLLNMNSPRTAGALELQNRSVQLDNYTPGAATNYHFQ
                     FFVPSTNNIGSIVYEFCTNSAIFAESCTAPAGLDVTAANITGQTGNTGFSIDNADTTV
                     NQLVITRPSFAGSIITTTYDFSGITDSLTAGQTIFVRVSTHASIDGSGATNDSGGVAY
                     VTGASLNVAAQVPPFLRLCVGLTVAPDCSTASGDSLDLGDLSTNHASAGQSQFATGTN
                     AVSGYVIYALGTTMTSGNDVIPALTTPTPSFPGNDQFGINLRANSNPASGQDPSGAGT
                     GSPTPDYDINDQFVFNNGDTIASSSLPTDYNRMTVSYLVNINHAQNIGIYSTTITYVA
                     TVTF"
     gene            3177..4148
                     /locus_tag="VEH48_02970"
     CDS             3177..4148
                     /locus_tag="VEH48_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18352.1"
                     /translation="MQTFKRLLTAVASLLLLLSSMPLVSAQATASGSGLSISPTLFEY
                     TLTPGQSANLSITLKDATVGAIDAQAVVNDFVADGTTGNPKILTQPGASSPNSIKNFV
                     YQLSNVPLNPGQQKIVTVGIHIPDGTAPGAYYGVIRYKAVPTGAAAPAPGQVALTASV
                     GTIVLITVPGNLRTLVKLNSLHIYHGTQDGHFFLNKPDHIGVEIQNLGNGFIRPFGTV
                     EVQNMFHREIDSFQFNNPKQLGNVLPNSTRIFTNAVSGISQPGRYTVSASVAYTSGGN
                     VLTIKKTFWYIPTWLALTIAAILLILILLTIRLYVRYRRDARHSYRR"
     gene            4188..5063
                     /locus_tag="VEH48_02975"
     CDS             4188..5063
                     /locus_tag="VEH48_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HXY18353.1"
                     /translation="MRFSIRPAIVLCITAATLPAILMPAAAYAVTQQSGSVGIQATLP
                     GPAPNQAPTIDIPSNGQSFVNLPIKVSGLCQSGLLVEVYKNNVFTGSVVCAGGSYSLQ
                     IDLFSGRNDLVARQYDALDQASPDSNTVSVTYNTSFAGNAPQVLITTQYAKRGADPGS
                     VLTWPLSISGGNAPYAVSIDWGDKTAFELLSLAKPGDFNAVHTYNAAGTYNITVKATD
                     ANGNTAFLQLVGVGNGPITQTTKAANQNVIIEKEIIWWPFILLVLLAITGFWLGRKHQ
                     LDTIRSRLRRGQRPF"
     gene            5109..6194
                     /gene="mnmA"
                     /locus_tag="VEH48_02980"
     CDS             5109..6194
                     /gene="mnmA"
                     /locus_tag="VEH48_02980"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXY18354.1"
                     /translation="MKTVFVGLSGGVDSSVSAALLKQQGYKVVGVYMKNWSRDLPGMP
                     CPWLDDYQDAKRVAVHLGIDFKMYDFEKDYQHKVVDYMLDEFKAGRTPNPDIMCNQEV
                     KFKLFLNTALEDGADLIATGHYAKIGDGGKGIGYSLLRAKDENKDQTYFLYRISQEAL
                     KHTIFPLGNVTKPQVRELARKYKLVTADKKESMGICFVGKVPIKDFLQQFIKTRPGKI
                     IDQAGREVGTHDGAIFYTIGQRHGLEVGGGLPYYVTGKDMKKNEVYVTTDLQDEKLWS
                     REVRLTDMHWINKIGDMGKGIGDRKLQVRLRHRAKLIDVKMLDLLSNSKAKAELAEEV
                     RALTPGQSAVFYSGTECLGGGIVVQAL"
     gene            complement(6117..6527)
                     /locus_tag="VEH48_02985"
     CDS             complement(6117..6527)
                     /locus_tag="VEH48_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18355.1"
                     /translation="MSLRAEDMLEVQGVAADYDRVSQEGAKRAELLRLAGVVIEVATS
                     NFFVETFRPGYPLGVKVAEAVHSVHSDSMDEVMELKKMAEEARVLIARLASREQNISD
                     SAAATRDQSLKRLDDDAAAQAFGAGVEDGRLAGS"
     gene            6590..8500
                     /locus_tag="VEH48_02990"
     CDS             6590..8500
                     /locus_tag="VEH48_02990"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF002436.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXY18356.1"
                     /translation="MTAQEIRKKYLEFMAARGHKIIPRASLVPQDDPTTLFTGSGMQP
                     LVPYLLGEPHPDGKRLADSQTCFRAEDMDEVGDNRHTTFFEMLGNWSLGDYFKKEQIP
                     WFFEFLTEVIGLEPAKLYVTCFIGDEDKGVHKDTESSEIWQRLFNSKNIEAKTGDIGS
                     QENGYDRGMKPGERIFYYDASKNWWSREGEPGHMREGEPGGPDSEVFYDFGTPHDEKF
                     GKYCHPNCDCGRFLEIGNSVFMTYKKIGDKFEHLPEPNVDFGGGLERIAAAAQHDPDI
                     FKISLIWPVIEGLEDLSGKKYEEHQHAMRVIADHLRAAVFLAVDGVVPSNKTQGYVLR
                     RLLRRAIRYALELGIEQDLCDKVADIIVQLYKPDFPEVEQREKDVLEAFAREEKLFRQ
                     TLRSGLKELDKILMLDEMQPKIRYNQVLTGEILFKLYDTYGFPIELSKEEAKKKDIEI
                     AKNADEEFKKLMEEQRQRSQTASQGMFKGGLADHSEQVIKYHTATHLMYAALKQVLGD
                     HVVQRGSNITAERTRFDFSHPEKVTPEQIAEVEKIVNAKITEDLPVTWKEEDTKEAFA
                     KGASGAFGDKYGDKVKVYTIGDPAKPFSREICGGPHMEHTGQIGEGGKTFKIVKEESS
                     SAGIRRIKAVLE"
     gene            8656..>8879
                     /locus_tag="VEH48_02995"
     CDS             8656..>8879
                     /locus_tag="VEH48_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXY18357.1"
                     /translation="MLLCAGVILALWTFRAVADDIHVTAKVPANPVTGPAVITNPVNG
                     QHFSSIPITISGTCPSDGSGGYVKIYRNDV"
BASE COUNT         2275 a         2401 c         2167 g         2036 t
ORIGIN      
        1 gcgagccctt tttatcgatg acggtcaaca cctcctcggt caatctgggc aaccttagca
       61 cttccacaac cgcaactggt atcgcgactt ttgacgtgac agcctacgta gacagcggct
      121 ataccgtcca aaccgtcagc cagccgccga gttataccag cggcagtagt tctcatacct
      181 tgaccgctat gagcctgggt ggttcggtgg tcggcaccga gcaatacggc attaacctgg
      241 ttgttaatac ctcgccggct tcgttcggct ctaacccggt accggaacca agcagtgctt
      301 tcgccaacgg cgctgcggcc accggctaca acacgcccaa tgaatacgct tacaatgccg
      361 gtgacgtcat cgcctgtgcc ggcaccagcg gcagctgcac tggcagcggc tggggcgata
      421 cgaccttcac tatttcttat atcgccaaca tttctttggt gacgccggct ggaaactaca
      481 gcgcaatcca agacctggta gttgtcgcga cttactaagg tatagaaaga cagctaaatc
      541 cggccttgcg ccgtaatagc gctcatgcta taaataagca atagaaactg taggagttta
      601 acaattaaca caaacaactc cggccgcttt gagcggctgg cctctcgcta tctagataaa
      661 ttgtcatacc gcaggcatct ttataaaatt cctgcttttt tcgtaaccgt tattctggct
      721 actatgtggc tggtaccctt tgtggtgccc aatataaccg acgctcaaca attaactctc
      781 cggtcgctta ctatgtccag cggcgtgcca agcaacagcg gtgttaataa cacctatacc
      841 tttggattta ctaccggctc ttccgcaacc gggactctgc aaggcatgaa acttgtcgcc
      901 tgccaaacgg cagtccagac ttatggcttt gcctcttcgg ccagcactac tggttgcgtg
      961 gcgccaactg gtttgacttt cgccagcgcc gtttgggtta gccaaaccgg tattctccct
     1021 actggctctc cggtcaactt tgccgttgat ggcaccggcg gcggtttctg cacgccggcc
     1081 gctaatgtct tatgcgccaa acgatccagc gccaccaccc agaccgccag cttggcgata
     1141 accattaaat ttagcaccat aacaaaccca tccttagcag ggggcaactg cagtgctaac
     1201 ggctgtagtt tctatatcgg tatatacacc taccagacat cgtacgcctc gccgattgac
     1261 tccggtaccg ttgctggtgc ggtagtccag accttgcaag tcaccgccgt cgtagctgaa
     1321 atcttgcagt tctgtgtagg ctcaacatca gttgacgatg ctaccacagc tgtaggtaat
     1381 gccagcagct gctcaggcac tacagttgac ctaggtactc taaatccgac cgccattaac
     1441 tacccaatta ctaacggtac cagccctaat aacgtaaatg gcgtagccat tttgcgaacc
     1501 aatgccataa atggctcgtc agtaaactat gattcaatac aagatactga tactgggagt
     1561 gggcagcacg gtgaccttaa aatccaaggg actaactgcg taggaggtgt gacaccaaac
     1621 cccaacgctt gtattacgtc caaagcttcc caaggggtat ttaccgcagg aacttcagac
     1681 tttggtatga ctataggcgg cactaactgc ggcacagaaa ccgcgggcgg ctactatacc
     1741 tgctcttact ctggcggtag cgaacatctt gtaccgcaaa ctggatacat cggttgggcg
     1801 agtaactatt gtggcgaagc aacctgtaat gcacacaatg gttttaaatg ggacgacaca
     1861 ggcacatcag atacccttgc cgtcgcagca tctaacaatg ttgtagccga tgaggcgcta
     1921 attctacaat tcgccgctac cccatcgatt accaatacct ttggccctta ctctgtaaaa
     1981 gttgacttta cggccctccc cactttttaa aagtgcatcg gcccggtacc gatgagaaat
     2041 ttcattaaat cccgacttgg aacaagtcct atatattttt tagcggtgct gatttttatc
     2101 ggcctgtggc tgcttaatat gaactccccg cggactgctg gcgccttgga gctacagaac
     2161 cgctccgtgc agctggacaa ttacaccccc ggtgccgcta ccaattatca ttttcagttt
     2221 tttgtgccca gcactaataa catcggttca atcgtttatg aattctgtac caactcggcg
     2281 atatttgctg aaagttgcac agcacccgct ggcttggacg tcacggcggc gaacatcacc
     2341 ggccaaaccg gcaataccgg ctttagcatt gataatgccg atactacggt taatcagttg
     2401 gtaataacca ggccttcctt tgccggctct atcattacca cgacatacga ttttagcggc
     2461 attaccgact cattaactgc cggacagacg atttttgtaa gagtttcaac ccacgccagc
     2521 attgacggca gcggcgccac caacgacagc ggcggtgtgg cctatgttac cggggcttca
     2581 ctgaacgtag cggcccaggt gccgccgttt ttgcggctgt gtgttggcct gacggtagcg
     2641 ccggactgtt ccacggccag cggcgacagc ctggacctgg gcgacctctc aaccaatcac
     2701 gccagcgccg gccaatcgca gtttgctacc ggcaccaatg ccgtcagcgg ctatgtgatt
     2761 tacgcgctgg ggactactat gacatccggt aacgatgtga ttccagctct gacaacgccc
     2821 acacccagtt ttcccggtaa cgaccagttc ggtatcaatt tgcgggccaa ttcaaacccg
     2881 gcctcaggcc aggacccatc cggcgccggt acgggtagcc cgacgcctga ttatgacatt
     2941 aatgaccagt tcgtttttaa caatggcgat accattgcca gttcttcctt gccaaccgac
     3001 tacaaccgca tgacggtttc gtatctggta aacatcaacc atgcgcaaaa tatcggcata
     3061 tacagcacca ccatcaccta cgtggcaacg gtaactttct aaaatgatgc tattcaaaga
     3121 gctgacacct ttgttatact tatcgcgaca gctaaatgaa cattaccaga gggtaaatgc
     3181 aaacttttaa gaggttatta acagccgttg caagcctgct gttgctattg tcttctatgc
     3241 ctttggtcag cgcccaggcg accgccagcg gtagcggcct aagcatctca ccgaccctat
     3301 ttgaatacac tctgacaccc ggtcaatcgg ctaatttgag cattactctc aaagacgcca
     3361 cagtcggggc cattgacgcc caagctgtag tgaacgattt cgtggctgat ggcactaccg
     3421 gcaaccccaa aattttgacc caacccggtg cttccagtcc caacagcatc aaaaattttg
     3481 tctaccagct cagtaacgtg ccgcttaacc cgggtcagca aaaaatcgtc accgttggta
     3541 tccatattcc agacggtacg gcacccggtg cgtattatgg agtaatccgt tataaagcag
     3601 taccgaccgg cgccgcggcg ccggctccgg gccaggtagc cttaaccgcc agcgtcggca
     3661 ccattgttct aataaccgtc cctggtaatt tacgtacgct cgtgaaactc aacagccttc
     3721 acatttatca cggcacccag gacggtcact ttttcctaaa caagcccgac catatcggcg
     3781 ttgagattca aaatctaggc aacggcttca tccggccgtt cggtacagta gaagtccaga
     3841 acatgttcca tagagaaatt gattccttcc agtttaacaa tcccaaacag ttgggcaacg
     3901 tcctgcctaa cagtacacgt atctttacta acgctgtttc cggcatcagc cagcccggcc
     3961 gctacacagt tagcgccagt gtggcctaca ccagcggcgg caacgtcttg accattaaaa
     4021 agactttttg gtacatcccg acatggttag cactgaccat agccgctatc ctgttaatcc
     4081 taatcttgtt aactatccgc ctatatgttc gctaccggcg tgacgccaga cactcttatc
     4141 gaagatagta gggtcataag gtgggcattg ccaaaaccac ggctaaaatg agattctcta
     4201 tccgcccagc catagtccta tgcataacgg cagcgacgtt gccagcaatc ctgatgcccg
     4261 cggccgctta cgctgtcaca caacaatccg gctcggtggg catccaggct acactgcctg
     4321 ggccggctcc taatcaggcg ccgaccatag acattcccag caacggccag agtttcgtaa
     4381 acctaccgat aaaagtctcg ggtctttgcc aaagcggtct gctggttgag gtttacaaga
     4441 acaatgtctt tactggttcg gtggtttgcg ccggcggcag ctactctttg caaattgatc
     4501 tttttagcgg ccgcaatgac ttggtggcta ggcagtatga cgcgcttgac caagccagtc
     4561 ccgactccaa caccgtcagt gtcacgtaca atacgtcctt tgccggcaat gcaccgcaag
     4621 tactgataac tacgcaatat gccaagcgcg gagcggatcc gggcagtgtt ctcacctggc
     4681 cgctgtccat aagcggcggc aacgcgccgt acgccgttag cattgattgg ggtgacaaaa
     4741 cggcgtttga acttctaagc ttggccaagc cgggcgattt taatgccgtc catacttaca
     4801 acgccgccgg cacctataac atcacggtca aagcgaccga cgccaacggc aacaccgcct
     4861 ttttacagtt ggtcggcgtc ggcaatggtc ccataaccca gaccaccaaa gccgccaacc
     4921 agaatgtcat catagagaag gaaattatct ggtggccatt tatcctgctg gtactgctgg
     4981 caataacggg tttctggctg gggcgcaaac accagctaga caccattaga agccgcctcc
     5041 gaagaggcca acgcccgttc taataccctc ttaccctaca cccctacaac cctttataat
     5101 aatttcagat gaaaactgtg tttgtcggtt tgtccggcgg ggttgatagc tccgtcagcg
     5161 ccgctttgct caagcagcaa ggctacaagg tcgttggtgt ctacatgaag aactggagcc
     5221 gtgacctgcc gggcatgccg tgcccgtggc tggacgacta ccaggacgct aagcgcgtgg
     5281 ccgtacatct gggcattgat tttaaaatgt acgactttga gaaagattac cagcacaaag
     5341 tcgtggacta catgctggat gaattcaagg ccggacgcac gcccaacccg gacatcatgt
     5401 gcaaccagga agtcaagttc aaactctttt tgaatacggc gctggaggac ggcgctgatt
     5461 tgattgccac cggccattac gcgaagatag gggatggggg taaggggata gggtatagtt
     5521 tgctgcgcgc gaaagatgaa aacaaggacc agacttattt tctttatcgg atttcgcaag
     5581 aagcgttgaa gcatacgatt tttccgttgg gtaatgttac taagccgcaa gtcagggaat
     5641 tagcaagaaa atacaagctg gtcacggctg ataaaaaaga aagcatgggc atctgtttcg
     5701 tcggcaaggt gccgatcaaa gacttcctac aacagttcat caaaaccagg cccggtaaaa
     5761 ttattgatca ggcgggcagg gaagtcggca cgcacgacgg ggcgattttc tacaccatcg
     5821 gccagcgcca cggcttggag gtcggcggcg gcttgccata ttacgtcacc ggcaaagata
     5881 tgaagaagaa tgaagtttac gtaacaactg acttgcaaga cgaaaaactc tggagcaggg
     5941 aagtcagatt aacagatatg cattggataa acaaaatagg ggatatgggc aaggggatag
     6001 gggatagaaa gcttcaagtt cggttgcgac acagggcaaa acttatagat gtaaaaatgc
     6061 ttgacttgtt aagtaattct aaagccaagg ccgaacttgc ggaagaagtc cgggccctaa
     6121 ctcccggcca gtctgccgtc ttctactccg gcaccgaatg cctgggcggc ggcatcgtcg
     6181 tccaagcgct ttagtgattg gtccctggta gctgcagcac tatctgatat gttttgttcg
     6241 cgactagcta gcctggcaat aaggacacga gcctcttcgg ccatcttctt taattccatg
     6301 acttcgtcca tagagtcgga atgtacagaa tgaaccgcct cagcaacttt taccccgagg
     6361 ggatagccgg gtctgaaagt ctctacgaaa aaattgcttg ttgctacttc gatgacaacg
     6421 ccggctagtc ttaataattc ggctcgtttg gcgccctctt ggctgactct gtcgtaatca
     6481 gctgcaacgc cttggacttc cagcatatct tctgcccgta aactcattat agatactata
     6541 ttaccattct tgtgaaagtc aacactacat atctgttaca ataactttta tgacggcgca
     6601 ggaaatacgc aaaaagtatt tggagttcat ggccgcgcgc gggcacaaaa taatcccgcg
     6661 ggcttctttg gtgccccagg acgacccaac cactctgttc accggcagcg gcatgcagcc
     6721 gctggtgccg tatctgcttg gcgaacctca tcctgacggc aaacggttgg cggatagcca
     6781 gacctgcttc cgcgccgagg acatggacga ggtcggcgac aaccggcata ccacattttt
     6841 tgaaatgcta ggcaactgga gcctgggcga ctactttaaa aaagaacaga ttccctggtt
     6901 ctttgaattt ttaacagagg tcataggcct tgaaccagcc aagctgtacg taacctgttt
     6961 tatcggcgat gaagacaaag gcgtgcacaa agataccgag tcctcggaaa tttggcagag
     7021 gctttttaac tctaaaaata ttgaagccaa aaccggcgac atcggcagtc aggaaaacgg
     7081 ttatgaccgc gggatgaaac ccggtgagcg gattttctat tacgacgctt ctaagaactg
     7141 gtggagccgc gagggcgagc ccggccatat gcgcgaaggc gaaccaggcg gcccggacag
     7201 cgaagtcttt tacgacttcg gtacaccgca tgatgaaaag ttcggcaaat actgccatcc
     7261 taactgcgac tgcggccgct ttttagagat tggcaacagc gtgttcatga cctacaagaa
     7321 aatcggcgat aagtttgagc acctgcccga acccaacgtg gatttcggcg gcggcctgga
     7381 gcggatcgcc gcggccgccc agcatgaccc cgatatcttt aaaatcagcc taatctggcc
     7441 agttatagag ggtctggagg atttgagcgg caaaaaatac gaagagcatc aacacgccat
     7501 gcgggtgatt gccgatcact tgcgggccgc ggtttttctg gcggttgacg gcgttgtgcc
     7561 aagcaacaaa acccagggat atgtgctgcg ccgtctgctg cggcgggcta tccgctacgc
     7621 gcttgagctg gggatagagc aagatttatg cgacaaagtc gcagatataa ttgtacaact
     7681 ttacaaaccc gactttccgg aagttgagca gcgcgaaaaa gacgtgctgg aggctttcgc
     7741 ccgcgaggaa aaactcttcc gccaaaccct ccgtagcggg ctgaaagaac tagataaaat
     7801 tttgatgctc gatgagatgc aaccaaaaat aagatataac caagtactta ctggcgaaat
     7861 tttgtttaag ctttatgata cgtatggttt tccgatagaa cttagtaaag aggaagctaa
     7921 aaagaaagac attgaaattg ccaaaaatgc ggacgaagaa tttaaaaaac ttatggaaga
     7981 gcagaggcag cggagtcaga cagcaagcca gggcatgttc aagggcggtc tggcggatca
     8041 ttcggaacaa gtaatcaagt accacaccgc cacgcacttg atgtacgcgg cgctgaaaca
     8101 agtcctgggc gaccacgtgg tccagcgcgg cagcaacatc acagctgagc gtacccgttt
     8161 tgattttagc catccggaaa aagttacacc ggaacagatc gccgaagtag aaaaaatcgt
     8221 caatgccaaa attaccgaag atttaccggt aacctggaaa gaagaagata ccaaagaagc
     8281 ttttgccaaa ggcgcttccg gagcattcgg cgacaaatac ggcgacaaag ttaaggtcta
     8341 caccatcggc gacccggcca agccgttcag ccgcgaaatc tgcggtggcc cgcacatgga
     8401 gcacaccggc caaataggcg agggcggcaa gacgttcaaa atcgtcaaag aagaatcaag
     8461 ttctgcggga atccgccgca tcaaagcagt actagagtaa aaacccttta catcgctgcc
     8521 taaaagcgct agtgtttagg tgtccttctg caaaaataaa aatcggcaaa aatggtccgt
     8581 agaaaacctg tggcaaagcg ctattaccga tcccgccgaa accggttgtt cataccgtat
     8641 ccggcgatac tttttatgct gctctgcgcc ggtgtgatac ttgctctttg gacgttcagg
     8701 gccgtggcgg acgatatcca cgtgaccgcc aaagttcccg ctaaccctgt taccggtcca
     8761 gcggtgatta ccaatcccgt gaacggccag cacttttctt ctattccaat cactatctcc
     8821 ggcacctgcc cgtcagacgg ttccggcggc tacgttaaaa tttatcgcaa cgatgtttt
//
